Restriction map for
pF1KA0408
(Circular) (Six-base) MAPSORT of: pF1KA0408.seq Check: 3254 from: 1 to: 5954
pF1KA0408 5954 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2060 2060
Size: 5954
AccI GT'mk_AC
Cuts at: 2992 2992
Size: 5954
Acc65I G'GTAC_C
Cuts at: 2970 2970
Size: 5954
AclI AA'CG_TT
Cuts at: 5794 5794
Size: 5954
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 1702 1972 2242 3779 4211 4958 5226 500
Size: 1202 270 270 1537 432 747 268 1228
Fragments arranged by size:
1537 1228 1202 747 432 270 270 268
AfeI AGC'GCT
Cuts at: 2311 2311
Size: 5954
AflIII A'CryG_T
Cuts at: 3306 4410 5387 3306
Size: 1104 977 3873
Fragments arranged by size:
3873 1104 977
AgeI A'CCGG_T
Cuts at: 4342 4342
Size: 5954
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1639 2281 2776 1639
Size: 642 495 4817
Fragments arranged by size:
4817 642 495
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1760 1792 1760
Size: 32 5922
AlwNI CAG_nnn'CTG
Cuts at: 175 330 692 2052 3259 4826 175
Size: 155 362 1360 1207 1567 1303
Fragments arranged by size:
1567 1360 1303 1207 362 155
ApaI G_GGCC'C
Cuts at: 1060 2435 2519 1060
Size: 1375 84 4495
Fragments arranged by size:
4495 1375 84
ApaLI G'TGCA_C
Cuts at: 4724 4724
Size: 5954
ApoI r'AATT_y
Cuts at: 2958 5307 5563 2958
Size: 2349 256 3349
Fragments arranged by size:
3349 2349 256
AscI GG'CGCG_CC
Cuts at: 2230 2230
Size: 5954
AseI AT'TA_AT
Cuts at: 20 20
Size: 5954
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5954
AvaI C'yCGr_G
Cuts at: 535 548 577 2512 2782 2974 535
Size: 13 29 1935 270 192 3515
Fragments arranged by size:
3515 1935 270 192 29 13
BamHI G'GATC_C
Cuts at: 1328 2979 1328
Size: 1651 4303
BanI G'GyrC_C
Cuts at: 432 2180 2508 2787 2812 2970 3384 3632 3667
Size: 1748 328 279 25 158 414 248 35
Cuts at: 3667 432
Size: 2719
Fragments arranged by size:
2719 1748 414 328 279 248 158 35
25
BanII G_rGCy'C
Cuts at: 225 884 1060 1778 1868 2138 2252 2435 2519
Size: 659 176 718 90 270 114 183 84
Cuts at: 2519 2825 2968 3998 225
Size: 306 143 1030 2181
Fragments arranged by size:
2181 1030 718 659 306 270 183 176
143 114 90 84
BbeI G_GCGC'C
Cuts at: 436 2512 2816 3388 3636 436
Size: 2076 304 572 248 2754
Fragments arranged by size:
2754 2076 572 304 248
BbsI GAAGACnn'nnnn_
Cuts at: 2072 2913 2072
Size: 841 5113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1252 1286 1902 1936 2574 2608 1252
Size: 34 616 34 638 34 4598
Fragments arranged by size:
4598 638 616 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3852 4613 5954 3852
Size: 761 1341 3852
Fragments arranged by size:
3852 1341 761
BclI T'GATC_A
Cuts at: 3474 3474
Size: 5954
BglI GCCn_nnn'nGGC
Cuts at: 178 418 178
Size: 240 5714
BglII A'GATC_T
Cuts at: 1677 3469 5506 1677
Size: 1792 2037 2125
Fragments arranged by size:
2125 2037 1792
BlpI GC'TnA_GC
Cuts at: 716 2251 3066 716
Size: 1535 815 3604
Fragments arranged by size:
3604 1535 815
Bme1580I G_kGCm'C
Cuts at: 569 1060 2435 2519 2792 3579 3672 4728 569
Size: 491 1375 84 273 787 93 1056 1795
Fragments arranged by size:
1795 1375 1056 787 491 273 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 280 3166 3247 3582 5726 280
Size: 2886 81 335 2144 508
Fragments arranged by size:
2886 2144 508 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 765 2469 4321 765
Size: 1704 1852 2398
Fragments arranged by size:
2398 1852 1704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1438 1762 1951 2125 1438
Size: 324 189 174 5267
Fragments arranged by size:
5267 324 189 174
Bpu10I CC'TnA_GC
Cuts at: 1879 1879
Size: 5954
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1189 1414 1852 3130 4009 4501 4799 883
Size: 306 225 438 1278 879 492 298 2038
Fragments arranged by size:
2038 1278 879 492 438 306 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 1003 5658 1003
Size: 4655 1299
BsaAI yAC'GTr
Cuts at: 2646 3307 2646
Size: 661 5293
BsaBI GATnn'nnATC
Cuts at: 6 3468 5505 6
Size: 3462 2037 455
Fragments arranged by size:
3462 2037 455
BsaHI Gr'CG_yC
Cuts at: 424 433 2363 2509 2813 3385 3633 4335 5831
Size: 9 1930 146 304 572 248 702 1496
Cuts at: 5831 424
Size: 547
Fragments arranged by size:
1930 1496 702 572 547 304 248 146
9
BsaWI w'CCGG_w
Cuts at: 808 3143 3664 4342 4616 4763 5174 808
Size: 2335 521 678 274 147 411 1588
Fragments arranged by size:
2335 1588 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 1760 1790 544
Size: 30 1186 30 4708
Fragments arranged by size:
4708 1186 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 410 553 623 641 644 756 1183 1279 1678
Size: 143 70 18 3 112 427 96 399
Cuts at: 1678 2122 2230 2473 2695 2833 410
Size: 444 108 243 222 138 3531
Fragments arranged by size:
3531 444 427 399 243 222 143 138
112 108 96 70 18 3
BseYI C'CCAG_C
Cuts at: 1061 1846 3379 4137 4714 5210 1061
Size: 785 1533 758 577 496 1805
Fragments arranged by size:
1805 1533 785 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 5954
BsiEI CG_ry'CG
Cuts at: 104 929 2541 2598 2778 2808 3496 3542 4750
Size: 825 1612 57 180 30 688 46 1208
Cuts at: 4750 104
Size: 1308
Fragments arranged by size:
1612 1308 1208 825 688 180 57 46
30
BsiHKAI G_wGCw'C
Cuts at: 225 884 1778 1868 2138 2252 2620 2711 2968
Size: 659 894 90 270 114 368 91 257
Cuts at: 2968 3746 3936 4728 225
Size: 778 190 792 1451
Fragments arranged by size:
1451 894 792 778 659 368 270 257
190 114 91 90
BsiWI C'GTAC_G
Cuts at: 708 708
Size: 5954
BsmBI CGTCTCn'nnnn_
Cuts at: 2065 2065
Size: 5954
Bsp1286I G_dGCh'C
Cuts at: 225 569 884 1060 1778 1868 2138 2252 2435
Size: 344 315 176 718 90 270 114 183
Cuts at: 2435 2519 2620 2711 2792 2825 2968 3579 3672
Size: 84 101 91 81 33 143 611 93
Cuts at: 3672 3746 3936 3998 4728 225
Size: 74 190 62 730 1451
Fragments arranged by size:
1451 730 718 611 344 315 270 190
183 176 143 114 101 93 91 90
84 81 74 62 33
BspEI T'CCGG_A
Cuts at: 808 5174 808
Size: 4366 1588
BspHI T'CATG_A
Cuts at: 2009 5130 2009
Size: 3121 2833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2060 3004 3218 3520 3901 2060
Size: 944 214 302 381 4113
Fragments arranged by size:
4113 944 381 302 214
BsrBI CCG'CTC
Cuts at: 50 448 594 909 1088 2007 2564 4246 4300
Size: 398 146 315 179 919 557 1682 54
Cuts at: 4300 5784 5949 50
Size: 1484 165 55
Fragments arranged by size:
1682 1484 919 557 398 315 179 165
146 55 54
BsrDI GCAATG_nn'
Cuts at: 3866 3866
Size: 5954
BsrFI r'CCGG_y
Cuts at: 295 634 793 2597 3952 4133 4342 295
Size: 339 159 1804 1355 181 209 1907
Fragments arranged by size:
1907 1804 1355 339 209 181 159
BssHII G'CGCG_C
Cuts at: 2174 2230 2635 2174
Size: 56 405 5493
Fragments arranged by size:
5493 405 56
BssSI C'ACGA_G
Cuts at: 1297 1771 2613 2710 4225 4583 1297
Size: 474 842 97 1515 358 2668
Fragments arranged by size:
2668 1515 842 474 358 97
BstAPI GCAn_nnn'nTGC
Cuts at: 330 1290 2052 2715 3331 330
Size: 960 762 663 616 2953
Fragments arranged by size:
2953 960 762 663 616
BstBI TT'CG_AA
Cuts at: 4315 4315
Size: 5954
BstXI CCAn_nnnn'nTGG
Cuts at: 1908 1908
Size: 5954
BstYI r'GATC_y
Cuts at: 1 1328 1677 1789 1803 2979 3446 3469 3804
Size: 1327 349 112 14 1176 467 23 335
Cuts at: 3804 4050 5062 5148 5160 5506 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1327 1176 1012 467 449 349 346 335
246 112 86 23 14 12
Bsu36I CC'TnA_GG
Cuts at: 1972 1972
Size: 5954
BtgI C'CryG_G
Cuts at: 929 2340 2808 929
Size: 1411 468 4075
Fragments arranged by size:
4075 1411 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1994 2589 3904 4085 4248 1994
Size: 595 1315 181 163 3700
Fragments arranged by size:
3700 1315 595 181 163
BtsI GCAGTG_nn'
Cuts at: 3197 3197
Size: 5954
ClaI AT'CG_AT
Cuts at: 1217 1217
Size: 5954
DraI TTT'AAA
Cuts at: 2913 2953 2913
Size: 40 5914
DrdI GACnn_nn'nnGTC
Cuts at: 3660 4518 3660
Size: 858 5096
EaeI y'GGCC_r
Cuts at: 272 503 926 1016 1736 1997 2096 2163 2648
Size: 231 423 90 720 261 99 67 485
Cuts at: 2648 3539 3713 4104 4131 5476 272
Size: 891 174 391 27 1345 750
Fragments arranged by size:
1345 891 750 720 485 423 391 261
231 174 99 90 67 27
EagI C'GGCC_G
Cuts at: 926 3539 926
Size: 2613 3341
EarI CTCTTCn'nnn_
Cuts at: 774 1602 3977 4187 774
Size: 828 2375 210 2541
Fragments arranged by size:
2541 2375 828 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 416 1017 1066 1972 2146 4162 4472 4618 5764
Size: 601 49 906 174 2016 310 146 1146
Cuts at: 5764 416
Size: 606
Fragments arranged by size:
2016 1146 906 606 601 310 174 146
49
EcoICRI GAG'CTC
Cuts at: 223 882 1776 1866 2136 2250 2966 223
Size: 659 894 90 270 114 716 3211
Fragments arranged by size:
3211 894 716 659 270 114 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 1438 1702 1762 1951 1972 2125 2242 3779
Size: 938 264 60 189 21 153 117 1537
Cuts at: 3779 4211 4958 5226 500
Size: 432 747 268 1228
Fragments arranged by size:
1537 1228 938 747 432 268 264 189
153 117 60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1420 2212 1420
Size: 792 5162
EcoO109I rG'GnC_Cy
Cuts at: 746 1056 1057 1425 1840 2205 2431 2516 2658
Size: 310 1 368 415 365 226 85 142
Cuts at: 2658 3093 746
Size: 435 3607
Fragments arranged by size:
3607 435 415 368 365 310 226 142
85 1
EcoRI G'AATT_C
Cuts at: 2958 2958
Size: 5954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3973 3973
Size: 5954
FspI TGC'GCA
Cuts at: 1411 2467 3735 1411
Size: 1056 1268 3630
Fragments arranged by size:
3630 1268 1056
HaeII r_GCGC'y
Cuts at: 134 436 668 1114 2313 2512 2580 2816 3388
Size: 302 232 446 1199 199 68 236 572
Cuts at: 3388 3636 4658 5637 134
Size: 248 1022 979 451
Fragments arranged by size:
1199 1022 979 572 451 446 302 248
236 232 199 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 544 576 2308 2340 2386 2418 2548
Size: 32 261 32 1732 32 46 32 130
Cuts at: 2548 2580 3796 3826 3828 3858 251
Size: 32 1216 30 2 30 2347
Fragments arranged by size:
2347 1732 1216 261 130 46 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1279 2993 1279
Size: 1714 4240
HindIII A'AGCT_T
Cuts at: 1559 1559
Size: 5954
KasI G'GCGC_C
Cuts at: 432 2508 2812 3384 3632 432
Size: 2076 304 572 248 2754
Fragments arranged by size:
2754 2076 572 304 248
KpnI G_GTAC'C
Cuts at: 2974 2974
Size: 5954
MluI A'CGCG_T
Cuts at: 5387 5387
Size: 5954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1283 2015 2324 4625 4809 1283
Size: 732 309 2301 184 2428
Fragments arranged by size:
2428 2301 732 309 184
MscI TGG'CCA
Cuts at: 1738 2650 3715 1738
Size: 912 1065 3977
Fragments arranged by size:
3977 1065 912
MslI CAynn'nnrTG
Cuts at: 2644 2830 3943 4070 2644
Size: 186 1113 127 4528
Fragments arranged by size:
4528 1113 186 127
MspA1I CmG'CkG
Cuts at: 166 330 734 836 919 931 1692 2058 2255
Size: 164 404 102 83 12 761 366 197
Cuts at: 2255 2271 2342 2477 2810 3065 3379 3739 4752
Size: 16 71 135 333 255 314 360 1013
Cuts at: 4752 4997 166
Size: 245 1123
Fragments arranged by size:
1123 1013 761 404 366 360 333 314
255 245 197 164 135 102 83 71
16 12
NaeI GCC'GGC
Cuts at: 297 636 4135 297
Size: 339 3499 2116
Fragments arranged by size:
3499 2116 339
NarI GG'CG_CC
Cuts at: 433 2509 2813 3385 3633 433
Size: 2076 304 572 248 2754
Fragments arranged by size:
2754 2076 572 304 248
NgoMIV G'CCGG_C
Cuts at: 295 634 4133 295
Size: 339 3499 2116
Fragments arranged by size:
3499 2116 339
NotI GC'GGCC_GC
Cuts at: 926 926
Size: 5954
NspI r_CATG'y
Cuts at: 1101 2307 3007 3507 4414 1101
Size: 1206 700 500 907 2641
Fragments arranged by size:
2641 1206 907 700 500
PasI CC'CwG_GG
Cuts at: 686 686
Size: 5954
PciI A'CATG_T
Cuts at: 4410 4410
Size: 5954
PflMI CCAn_nnn'nTGG
Cuts at: 468 692 1302 468
Size: 224 610 5120
Fragments arranged by size:
5120 610 224
PfoI T'CCnGG_A
Cuts at: 450 2702 450
Size: 2252 3702
PmeI GTTT'AAAC
Cuts at: 2953 2953
Size: 5954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 234 266 1760 1792 5093 5125 234
Size: 32 1494 32 3301 32 1063
Fragments arranged by size:
3301 1494 1063 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1425 2658 1425
Size: 1233 4721
PspOMI G'GGCC_C
Cuts at: 1056 2431 2515 1056
Size: 1375 84 4495
Fragments arranged by size:
4495 1375 84
PspXI vC'TCGA_Gb
Cuts at: 2782 2782
Size: 5954
PstI C_TGCA'G
Cuts at: 171 335 875 1286 1337 1697 2057 2213 2240
Size: 164 540 411 51 360 360 156 27
Cuts at: 2240 2276 2765 3001 3686 171
Size: 36 489 236 685 2439
Fragments arranged by size:
2439 685 540 489 411 360 360 236
164 156 51 36 27
PvuI CG_AT'CG
Cuts at: 104 2541 104
Size: 2437 3517
PvuII CAG'CTG
Cuts at: 330 1692 2058 2271 3379 3739 330
Size: 1362 366 213 1108 360 2545
Fragments arranged by size:
2545 1362 1108 366 360 213
RsrII CG'GwC_CG
Cuts at: 529 4149 529
Size: 3620 2334
SacI G_AGCT'C
Cuts at: 225 884 1778 1868 2138 2252 2968 225
Size: 659 894 90 270 114 716 3211
Fragments arranged by size:
3211 894 716 659 270 114 90
SacII CC_GC'GG
Cuts at: 932 2343 2811 932
Size: 1411 468 4075
Fragments arranged by size:
4075 1411 468
SalI G'TCGA_C
Cuts at: 2991 2991
Size: 5954
SapI GCTCTTCn'nnn_
Cuts at: 3977 4187 3977
Size: 210 5744
SbfI CC_TGCA'GG
Cuts at: 2213 3001 2213
Size: 788 5166
ScaI AGT'ACT
Cuts at: 5471 5471
Size: 5954
SfcI C'TryA_G
Cuts at: 33 167 331 871 1282 1333 1693 2053 2209
Size: 134 164 540 411 51 360 360 156
Cuts at: 2209 2236 2272 2761 2997 3682 4675 4866 33
Size: 27 36 489 236 685 993 191 1121
Fragments arranged by size:
1121 993 685 540 489 411 360 360
236 191 164 156 134 51 36 27
SfoI GGC'GCC
Cuts at: 434 2510 2814 3386 3634 434
Size: 2076 304 572 248 2754
Fragments arranged by size:
2754 2076 572 304 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5954
SmaI CCC'GGG
Cuts at: 2514 2976 2514
Size: 462 5492
SmlI C'TyrA_G
Cuts at: 898 1204 1429 1867 2782 3109 4024 4516 4778
Size: 306 225 438 915 327 915 492 262
Cuts at: 4778 898
Size: 2074
Fragments arranged by size:
2074 915 915 492 438 327 306 262
225
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5954
SphI G_CATG'C
Cuts at: 1101 3007 3507 1101
Size: 1906 500 3548
Fragments arranged by size:
3548 1906 500
SrfI GCCC'GGGC
Cuts at: 2514 2514
Size: 5954
SspI AAT'ATT
Cuts at: 2909 2909
Size: 5954
StuI AGG'CCT
Cuts at: 1949 2746 1949
Size: 797 5157
StyI C'CwwG_G
Cuts at: 3088 3442 3088
Size: 354 5600
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 255 2215 3537 3670 4129 4338 255
Size: 1960 1322 133 459 209 1871
Fragments arranged by size:
1960 1871 1322 459 209 133
TatI w'GTAC_w
Cuts at: 1784 2144 2150 2943 5469 1784
Size: 360 6 793 2526 2269
Fragments arranged by size:
2526 2269 793 360 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5471 5503 5471
Size: 32 5922
Tth111I GACn'n_nGTC
Cuts at: 3751 3751
Size: 5954
XbaI T'CTAG_A
Cuts at: 2985 2985
Size: 5954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 469 3449 469
Size: 2980 2974
XhoI C'TCGA_G
Cuts at: 2782 2782
Size: 5954
XmaI C'CCGG_G
Cuts at: 2512 2974 2512
Size: 462 5492
XmnI GAAnn'nnTTC
Cuts at: 2746 2746
Size: 5954
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AvrII BaeI BbvCI BfrBI
BmgBI BmtI BsmI BsrGI BstEII BstZ17I CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NruI NsiI PacI PmlI PshAI PsiI PsrI SanDI
SexAI SfiI SgrAI SnaBI SwaI ZraI