Restriction map for
pF1KA0391
(Circular) (Six-base) MAPSORT of: pF1KA0391.seq Check: 8837 from: 1 to: 4814
pF1KA0391 4814 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1852 1852
Size: 4814
Acc65I G'GTAC_C
Cuts at: 1830 1830
Size: 4814
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 704 2639 3071 3818 4086 428
Size: 276 1935 432 747 268 1156
Fragments arranged by size:
1935 1156 747 432 276 268
AflII C'TTAA_G
Cuts at: 487 1162 1231 487
Size: 675 69 4070
Fragments arranged by size:
4070 675 69
AflIII A'CryG_T
Cuts at: 2166 3270 4247 2166
Size: 1104 977 2733
Fragments arranged by size:
2733 1104 977
AgeI A'CCGG_T
Cuts at: 3202 3202
Size: 4814
AhdI GACnn_n'nnGTC
Cuts at: 1381 1381
Size: 4814
AleI CACnn'nnGTG
Cuts at: 1702 1702
Size: 4814
AlwNI CAG_nnn'CTG
Cuts at: 2119 3686 2119
Size: 1567 3247
ApaLI G'TGCA_C
Cuts at: 3584 3584
Size: 4814
ApoI r'AATT_y
Cuts at: 396 465 900 1818 4167 4423 396
Size: 69 435 918 2349 256 787
Fragments arranged by size:
2349 918 787 435 256 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 1834 1834
Size: 4814
AvrII C'CTAG_G
Cuts at: 1386 1386
Size: 4814
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 423 1830 2244 2492 2527 423
Size: 1407 414 248 35 2710
Fragments arranged by size:
2710 1407 414 248 35
BanII G_rGCy'C
Cuts at: 160 870 1828 2858 160
Size: 710 958 1030 2116
Fragments arranged by size:
2116 1030 958 710
BbeI G_GCGC'C
Cuts at: 2248 2496 2248
Size: 248 4566
BbsI GAAGACnn'nnnn_
Cuts at: 742 1751 742
Size: 1009 3805
BciVI GTATCCnnnnn_n'
Cuts at: 868 1058 2712 3473 4814 868
Size: 190 1654 761 1341 868
Fragments arranged by size:
1654 1341 868 761 190
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4814
BglII A'GATC_T
Cuts at: 2329 4366 2329
Size: 2037 2777
BlpI GC'TnA_GC
Cuts at: 1926 1926
Size: 4814
Bme1580I G_kGCm'C
Cuts at: 185 2439 2532 3588 185
Size: 2254 93 1056 1411
Fragments arranged by size:
2254 1411 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1413 2026 2107 2442 4586 1413
Size: 613 81 335 2144 1641
Fragments arranged by size:
2144 1641 613 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 4814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1736 389
Size: 1347 3467
Bpu10I CC'TnA_GC
Cuts at: 377 377
Size: 4814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1990 2869 3361 3659 1990
Size: 879 492 298 3145
Fragments arranged by size:
3145 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1294 1547 4518 1294
Size: 253 2971 1590
Fragments arranged by size:
2971 1590 253
BsaAI yAC'GTr
Cuts at: 2167 2167
Size: 4814
BsaBI GATnn'nnATC
Cuts at: 6 2328 4365 6
Size: 2322 2037 455
Fragments arranged by size:
2322 2037 455
BsaHI Gr'CG_yC
Cuts at: 2245 2493 3195 4691 2245
Size: 248 702 1496 2368
Fragments arranged by size:
2368 1496 702 248
BsaWI w'CCGG_w
Cuts at: 511 2003 2524 3202 3476 3623 4034 511
Size: 1492 521 678 274 147 411 1291
Fragments arranged by size:
1492 1291 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 246 276 246
Size: 30 4784
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 487 487
Size: 4814
BseYI C'CCAG_C
Cuts at: 2239 2997 3574 4070 2239
Size: 758 577 496 2983
Fragments arranged by size:
2983 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 1504
Size: 4814
BsiEI CG_ry'CG
Cuts at: 104 2356 2402 3610 104
Size: 2252 46 1208 1308
Fragments arranged by size:
2252 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 870 1828 2606 2796 3588 870
Size: 958 778 190 792 2096
Fragments arranged by size:
2096 958 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1353 1353
Size: 4814
Bsp1286I G_dGCh'C
Cuts at: 160 185 870 1828 2439 2532 2606 2796 2858
Size: 25 685 958 611 93 74 190 62
Cuts at: 2858 3588 160
Size: 730 1386
Fragments arranged by size:
1386 958 730 685 611 190 93 74
62 25
BspEI T'CCGG_A
Cuts at: 4034 4034
Size: 4814
BspHI T'CATG_A
Cuts at: 924 1175 3990 924
Size: 251 2815 1748
Fragments arranged by size:
2815 1748 251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1529 1864 2078 2380 2761 1529
Size: 335 214 302 381 3582
Fragments arranged by size:
3582 381 335 302 214
BsrBI CCG'CTC
Cuts at: 50 3106 3160 4644 4809 50
Size: 3056 54 1484 165 55
Fragments arranged by size:
3056 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2726 2726
Size: 4814
BsrFI r'CCGG_y
Cuts at: 2812 2993 3202 2812
Size: 181 209 4424
Fragments arranged by size:
4424 209 181
BssSI C'ACGA_G
Cuts at: 1722 3085 3443 1722
Size: 1363 358 3093
Fragments arranged by size:
3093 1363 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2191 2191
Size: 4814
BstBI TT'CG_AA
Cuts at: 132 527 3175 132
Size: 395 2648 1771
Fragments arranged by size:
2648 1771 395
BstEII G'GTnAC_C
Cuts at: 1198 1198
Size: 4814
BstXI CCAn_nnnn'nTGG
Cuts at: 1784 1784
Size: 4814
BstYI r'GATC_y
Cuts at: 1 1839 2306 2329 2664 2910 3922 4008 4020
Size: 1838 467 23 335 246 1012 86 12
Cuts at: 4020 4366 1
Size: 346 449
Fragments arranged by size:
1838 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1223 1223
Size: 4814
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2764 2945 3108 2764
Size: 181 163 4470
Fragments arranged by size:
4470 181 163
BtsI GCAGTG_nn'
Cuts at: 1516 1533 2057 1516
Size: 17 524 4273
Fragments arranged by size:
4273 524 17
DraI TTT'AAA
Cuts at: 500 953 1813 500
Size: 453 860 3501
Fragments arranged by size:
3501 860 453
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3378 2520
Size: 858 3956
EaeI y'GGCC_r
Cuts at: 1095 2399 2573 2964 2991 4336 1095
Size: 1304 174 391 27 1345 1573
Fragments arranged by size:
1573 1345 1304 391 174 27
EagI C'GGCC_G
Cuts at: 2399 2399
Size: 4814
EarI CTCTTCn'nnn_
Cuts at: 148 1436 2837 3047 148
Size: 1288 1401 210 1915
Fragments arranged by size:
1915 1401 1288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3022 3332 3478 4624 3022
Size: 310 146 1146 3212
Fragments arranged by size:
3212 1146 310 146
EcoICRI GAG'CTC
Cuts at: 868 1826 868
Size: 958 3856
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 428 704 1736 2639 3071 3818 4086 389
Size: 39 276 1032 903 432 747 268 1117
Fragments arranged by size:
1117 1032 903 747 432 276 268 39
EcoO109I rG'GnC_Cy
Cuts at: 1953 1953
Size: 4814
EcoRI G'AATT_C
Cuts at: 396 465 1818 396
Size: 69 1353 3392
Fragments arranged by size:
3392 1353 69
EcoRV GAT'ATC
Cuts at: 307 307
Size: 4814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4814
FspI TGC'GCA
Cuts at: 2595 2595
Size: 4814
HaeII r_GCGC'y
Cuts at: 2248 2496 3518 4497 2248
Size: 248 1022 979 2565
Fragments arranged by size:
2565 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 1206 1238 1367 1399 1468 1500 2656
Size: 32 790 32 129 32 69 32 1156
Cuts at: 2656 2686 2688 2718 384
Size: 30 2 30 2480
Fragments arranged by size:
2480 1156 790 129 69 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1853 1853
Size: 4814
HindIII A'AGCT_T
Cuts at: 135 145 956 135
Size: 10 811 3993
Fragments arranged by size:
3993 811 10
KasI G'GCGC_C
Cuts at: 2244 2492 2244
Size: 248 4566
KpnI G_GTAC'C
Cuts at: 1834 1834
Size: 4814
MfeI C'AATT_G
Cuts at: 1640 1640
Size: 4814
MluI A'CGCG_T
Cuts at: 4247 4247
Size: 4814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 514 923 3485 3669 514
Size: 409 2562 184 1659
Fragments arranged by size:
2562 1659 409 184
MscI TGG'CCA
Cuts at: 1097 2575 1097
Size: 1478 3336
MslI CAynn'nnrTG
Cuts at: 188 881 1702 2803 2930 188
Size: 693 821 1101 127 2072
Fragments arranged by size:
2072 1101 821 693 127
MspA1I CmG'CkG
Cuts at: 215 1044 1137 1463 1635 1925 2239 2599 3612
Size: 829 93 326 172 290 314 360 1013
Cuts at: 3612 3857 215
Size: 245 1172
Fragments arranged by size:
1172 1013 829 360 326 314 290 245
172 93
NaeI GCC'GGC
Cuts at: 2995 2995
Size: 4814
NarI GG'CG_CC
Cuts at: 2245 2493 2245
Size: 248 4566
NdeI CA'TA_TG
Cuts at: 671 1737 671
Size: 1066 3748
NgoMIV G'CCGG_C
Cuts at: 2993 2993
Size: 4814
NspI r_CATG'y
Cuts at: 1404 1867 2367 3274 1404
Size: 463 500 907 2944
Fragments arranged by size:
2944 907 500 463
PasI CC'CwG_GG
Cuts at: 178 178
Size: 4814
PciI A'CATG_T
Cuts at: 3270 3270
Size: 4814
PflMI CCAn_nnn'nTGG
Cuts at: 166 166
Size: 4814
PfoI T'CCnGG_A
Cuts at: 1525 1656 1525
Size: 131 4683
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 4782
PsiI TTA'TAA
Cuts at: 826 826
Size: 4814
PstI C_TGCA'G
Cuts at: 1540 1861 2546 1540
Size: 321 685 3808
Fragments arranged by size:
3808 685 321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4814
PvuII CAG'CTG
Cuts at: 1044 1137 1463 1635 2239 2599 1044
Size: 93 326 172 604 360 3259
Fragments arranged by size:
3259 604 360 326 172 93
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 870 1828 870
Size: 958 3856
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4814
SapI GCTCTTCn'nnn_
Cuts at: 148 2837 3047 148
Size: 2689 210 1915
Fragments arranged by size:
2689 1915 210
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 4331 4331
Size: 4814
SfcI C'TryA_G
Cuts at: 33 569 1536 1857 2542 3535 3726 33
Size: 536 967 321 685 993 191 1121
Fragments arranged by size:
1121 993 967 685 536 321 191
SfoI GGC'GCC
Cuts at: 2246 2494 2246
Size: 248 4566
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SmaI CCC'GGG
Cuts at: 1836 1836
Size: 4814
SmlI C'TyrA_G
Cuts at: 487 1162 1231 1969 2884 3376 3638 487
Size: 675 69 738 915 492 262 1663
Fragments arranged by size:
1663 915 738 675 492 262 69
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
StuI AGG'CCT
Cuts at: 1576 1576
Size: 4814
StyI C'CwwG_G
Cuts at: 165 296 419 1386 1948 2302 165
Size: 131 123 967 562 354 2677
Fragments arranged by size:
2677 967 562 354 131 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2397 2530 2989 3198 2397
Size: 133 459 209 4013
Fragments arranged by size:
4013 459 209 133
TatI w'GTAC_w
Cuts at: 1205 4329 1205
Size: 3124 1690
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4331 4363 4331
Size: 32 4782
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2309
Size: 4814
XmaI C'CCGG_G
Cuts at: 1834 1834
Size: 4814
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I BtgI ClaI CspCI DraIII EcoNI
FseI FspAI HpaI NcoI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI