Restriction map for
pF1KA0384
(Circular) (Six-base) MAPSORT of: pF1KA0384.seq Check: 5731 from: 1 to: 5930
pF1KA0384 5930 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 130 864 2968 130
Size: 734 2104 3092
Fragments arranged by size:
3092 2104 734
Acc65I G'GTAC_C
Cuts at: 2946 2946
Size: 5930
AclI AA'CG_TT
Cuts at: 5770 5770
Size: 5930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1535 3755 4187 4934 5202 1535
Size: 2220 432 747 268 2263
Fragments arranged by size:
2263 2220 747 432 268
AflII C'TTAA_G
Cuts at: 2122 2122
Size: 5930
AflIII A'CryG_T
Cuts at: 234 3282 4386 5363 234
Size: 3048 1104 977 801
Fragments arranged by size:
3048 1104 977 801
AgeI A'CCGG_T
Cuts at: 4318 4318
Size: 5930
AhdI GACnn_n'nnGTC
Cuts at: 641 2201 641
Size: 1560 4370
AlwNI CAG_nnn'CTG
Cuts at: 3235 4802 3235
Size: 1567 4363
ApaI G_GGCC'C
Cuts at: 884 884
Size: 5930
ApaLI G'TGCA_C
Cuts at: 1378 4700 1378
Size: 3322 2608
ApoI r'AATT_y
Cuts at: 343 2412 2768 2934 5283 5539 343
Size: 2069 356 166 2349 256 734
Fragments arranged by size:
2349 2069 734 356 256 166
AseI AT'TA_AT
Cuts at: 20 2344 20
Size: 2324 3606
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5930
AvaI C'yCGr_G
Cuts at: 188 803 2066 2950 188
Size: 615 1263 884 3168
Fragments arranged by size:
3168 1263 884 615
AvrII C'CTAG_G
Cuts at: 715 715
Size: 5930
BamHI G'GATC_C
Cuts at: 453 2019 2955 453
Size: 1566 936 3428
Fragments arranged by size:
3428 1566 936
BanI G'GyrC_C
Cuts at: 209 855 1055 1092 2946 3360 3608 3643 209
Size: 646 200 37 1854 414 248 35 2496
Fragments arranged by size:
2496 1854 646 414 248 200 37 35
BanII G_rGCy'C
Cuts at: 884 1119 1401 2081 2944 3974 884
Size: 235 282 680 863 1030 2840
Fragments arranged by size:
2840 1030 863 680 282 235
BbeI G_GCGC'C
Cuts at: 213 1059 3364 3612 213
Size: 846 2305 248 2531
Fragments arranged by size:
2531 2305 846 248
BbsI GAAGACnn'nnnn_
Cuts at: 550 1079 1315 1655 550
Size: 529 236 340 4825
Fragments arranged by size:
4825 529 340 236
BbvCI CC'TCA_GC
Cuts at: 2157 2157
Size: 5930
BciVI GTATCCnnnnn_n'
Cuts at: 3828 4589 5930 3828
Size: 761 1341 3828
Fragments arranged by size:
3828 1341 761
BclI T'GATC_A
Cuts at: 1610 2528 3450 1610
Size: 918 922 4090
Fragments arranged by size:
4090 922 918
BglI GCCn_nnn'nGGC
Cuts at: 948 948
Size: 5930
BglII A'GATC_T
Cuts at: 3445 5482 3445
Size: 2037 3893
BlpI GC'TnA_GC
Cuts at: 3042 3042
Size: 5930
Bme1580I G_kGCm'C
Cuts at: 858 884 1382 3555 3648 4704 858
Size: 26 498 2173 93 1056 2084
Fragments arranged by size:
2173 2084 1056 498 93 26
BmgBI CAC'GTC
Cuts at: 216 1317 1423 216
Size: 1101 106 4723
Fragments arranged by size:
4723 1101 106
BmrI ACTGGGnnnn_n'
Cuts at: 165 1040 1122 1333 1949 3142 3223 3558 5702
Size: 875 82 211 616 1193 81 335 2144
Cuts at: 5702 165
Size: 393
Fragments arranged by size:
2144 1193 875 616 393 335 211 82
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1754 2010 2081 2153 4297 1754
Size: 256 71 72 2144 3387
Fragments arranged by size:
3387 2144 256 72 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1980 2183 2321 217
Size: 1763 203 138 3826
Fragments arranged by size:
3826 1763 203 138
Bpu10I CC'TnA_GC
Cuts at: 2157 2157
Size: 5930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 1387 3106 3985 4477 4775 1312
Size: 75 1719 879 492 298 2467
Fragments arranged by size:
2467 1719 879 492 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 249 430 481 514 2834 5634 249
Size: 181 51 33 2320 2800 545
Fragments arranged by size:
2800 2320 545 181 51 33
BsaAI yAC'GTr
Cuts at: 3283 3283
Size: 5930
BsaBI GATnn'nnATC
Cuts at: 6 767 923 2110 3444 5481 6
Size: 761 156 1187 1334 2037 455
Fragments arranged by size:
2037 1334 1187 761 455 156
BsaHI Gr'CG_yC
Cuts at: 210 1056 3361 3609 4311 5807 210
Size: 846 2305 248 702 1496 333
Fragments arranged by size:
2305 1496 846 702 333 248
BsaWI w'CCGG_w
Cuts at: 1525 2729 3119 3640 4318 4592 4739 5150 1525
Size: 1204 390 521 678 274 147 411 2305
Fragments arranged by size:
2305 1204 678 521 411 390 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 463 835 1102 1743 2394 2852 214
Size: 249 372 267 641 651 458 3292
Fragments arranged by size:
3292 651 641 458 372 267 249
BseYI C'CCAG_C
Cuts at: 835 1127 2185 3355 4113 4690 5186 835
Size: 292 1058 1170 758 577 496 1579
Fragments arranged by size:
1579 1170 1058 758 577 496 292
BsiEI CG_ry'CG
Cuts at: 104 134 309 2866 3472 3518 4726 104
Size: 30 175 2557 606 46 1208 1308
Fragments arranged by size:
2557 1308 1208 606 175 46 30
BsiHKAI G_wGCw'C
Cuts at: 1382 1401 1688 2081 2307 2944 3722 3912 4704
Size: 19 287 393 226 637 778 190 792
Cuts at: 4704 1382
Size: 2608
Fragments arranged by size:
2608 792 778 637 393 287 226 190
19
BsmBI CGTCTCn'nnnn_
Cuts at: 222 222
Size: 5930
Bsp1286I G_dGCh'C
Cuts at: 858 884 1119 1382 1401 1688 2081 2307 2944
Size: 26 235 263 19 287 393 226 637
Cuts at: 2944 3555 3648 3722 3912 3974 4704 858
Size: 611 93 74 190 62 730 2084
Fragments arranged by size:
2084 730 637 611 393 287 263 235
226 190 93 74 62 26 19
BspEI T'CCGG_A
Cuts at: 5150 5150
Size: 5930
BspHI T'CATG_A
Cuts at: 1061 5106 1061
Size: 4045 1885
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1283 2980 3194 3496 3877 1283
Size: 1697 214 302 381 3336
Fragments arranged by size:
3336 1697 381 302 214
BsrBI CCG'CTC
Cuts at: 50 825 1635 2216 2549 4222 4276 5760 5925
Size: 775 810 581 333 1673 54 1484 165
Cuts at: 5925 50
Size: 55
Fragments arranged by size:
1673 1484 810 775 581 333 165 55
54
BsrDI GCAATG_nn'
Cuts at: 672 1302 3842 672
Size: 630 2540 2760
Fragments arranged by size:
2760 2540 630
BsrFI r'CCGG_y
Cuts at: 293 308 3928 4109 4318 293
Size: 15 3620 181 209 1905
Fragments arranged by size:
3620 1905 209 181 15
BssSI C'ACGA_G
Cuts at: 1496 4201 4559 1496
Size: 2705 358 2867
Fragments arranged by size:
2867 2705 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1591 3307 1591
Size: 1716 4214
BstBI TT'CG_AA
Cuts at: 1488 4291 1488
Size: 2803 3127
BstEII G'GTnAC_C
Cuts at: 807 807
Size: 5930
BstXI CCAn_nnnn'nTGG
Cuts at: 144 144
Size: 5930
BstYI r'GATC_y
Cuts at: 1 453 918 1886 2019 2871 2955 3422 3445
Size: 452 465 968 133 852 84 467 23
Cuts at: 3445 3780 4026 5038 5124 5136 5482 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 968 852 467 465 452 449 346
335 246 133 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 458 1708 458
Size: 1250 4680
BtgI C'CryG_G
Cuts at: 107 524 107
Size: 417 5513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 335 910 3880 4061 4224 335
Size: 575 2970 181 163 2041
Fragments arranged by size:
2970 2041 575 181 163
BtsI GCAGTG_nn'
Cuts at: 701 786 1016 1585 3173 701
Size: 85 230 569 1588 3458
Fragments arranged by size:
3458 1588 569 230 85
ClaI AT'CG_AT
Cuts at: 2862 2862
Size: 5930
DraI TTT'AAA
Cuts at: 2929 2929
Size: 5930
DrdI GACnn_nn'nnGTC
Cuts at: 3636 4494 3636
Size: 858 5072
EaeI y'GGCC_r
Cuts at: 272 306 3515 3689 4080 4107 5452 272
Size: 34 3209 174 391 27 1345 750
Fragments arranged by size:
3209 1345 750 391 174 34 27
EagI C'GGCC_G
Cuts at: 306 3515 306
Size: 3209 2721
EarI CTCTTCn'nnn_
Cuts at: 3953 4163 3953
Size: 210 5720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4138 4448 4594 5740 4138
Size: 310 146 1146 4328
Fragments arranged by size:
4328 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1399 2079 2942 1399
Size: 680 863 4387
Fragments arranged by size:
4387 863 680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1535 1980 2183 2321 3755 4187 4934 5202
Size: 1318 445 203 138 1434 432 747 268
Cuts at: 5202 217
Size: 945
Fragments arranged by size:
1434 1318 945 747 445 432 268 203
138
EcoO109I rG'GnC_Cy
Cuts at: 673 881 1177 3069 673
Size: 208 296 1892 3534
Fragments arranged by size:
3534 1892 296 208
EcoRI G'AATT_C
Cuts at: 2934 2934
Size: 5930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1399 3949 1399
Size: 2550 3380
FspI TGC'GCA
Cuts at: 1168 3711 1168
Size: 2543 3387
HaeII r_GCGC'y
Cuts at: 196 213 1059 2193 3364 3612 4634 5613 196
Size: 17 846 1134 1171 248 1022 979 513
Fragments arranged by size:
1171 1134 1022 979 846 513 248 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 595 627 659 1081 1113 1602 1634
Size: 32 451 32 32 422 32 489 32
Cuts at: 1634 1663 1695 2187 2219 3772 3802 3804 3834
Size: 29 32 492 32 1553 30 2 30
Cuts at: 3834 112
Size: 2208
Fragments arranged by size:
2208 1553 492 489 451 422 32 32
32 32 32 32 32 30 30 29
2
HincII GTy'rAC
Cuts at: 131 2969 131
Size: 2838 3092
HindIII A'AGCT_T
Cuts at: 1152 1828 2491 1152
Size: 676 663 4591
Fragments arranged by size:
4591 676 663
KasI G'GCGC_C
Cuts at: 209 1055 3360 3608 209
Size: 846 2305 248 2531
Fragments arranged by size:
2531 2305 846 248
KpnI G_GTAC'C
Cuts at: 2950 2950
Size: 5930
MluI A'CGCG_T
Cuts at: 234 5363 234
Size: 5129 801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1006 4601 4785 1006
Size: 3595 184 2151
Fragments arranged by size:
3595 2151 184
MscI TGG'CCA
Cuts at: 274 3691 274
Size: 3417 2513
MslI CAynn'nnrTG
Cuts at: 2699 3919 4046 2699
Size: 1220 127 4583
Fragments arranged by size:
4583 1220 127
MspA1I CmG'CkG
Cuts at: 228 1267 1699 2161 3041 3355 3715 4728 4973
Size: 1039 432 462 880 314 360 1013 245
Cuts at: 4973 228
Size: 1185
Fragments arranged by size:
1185 1039 1013 880 462 432 360 314
245
NaeI GCC'GGC
Cuts at: 295 4111 295
Size: 3816 2114
NarI GG'CG_CC
Cuts at: 210 1056 3361 3609 210
Size: 846 2305 248 2531
Fragments arranged by size:
2531 2305 846 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5930
NdeI CA'TA_TG
Cuts at: 2700 2700
Size: 5930
NgoMIV G'CCGG_C
Cuts at: 293 4109 293
Size: 3816 2114
NspI r_CATG'y
Cuts at: 1594 1951 2359 2983 3483 4390 1594
Size: 357 408 624 500 907 3134
Fragments arranged by size:
3134 907 624 500 408 357
PciI A'CATG_T
Cuts at: 4386 4386
Size: 5930
PflMI CCAn_nnn'nTGG
Cuts at: 639 639
Size: 5930
PmeI GTTT'AAAC
Cuts at: 2929 2929
Size: 5930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5069 5101 5069
Size: 32 5898
PpuMI rG'GwC_Cy
Cuts at: 673 673
Size: 5930
PshAI GACnn'nnGTC
Cuts at: 526 1049 526
Size: 523 5407
PspOMI G'GGCC_C
Cuts at: 880 880
Size: 5930
PspXI vC'TCGA_Gb
Cuts at: 2066 2066
Size: 5930
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 712 744 712
Size: 32 5898
PstI C_TGCA'G
Cuts at: 2977 3662 2977
Size: 685 5245
PvuI CG_AT'CG
Cuts at: 104 2866 104
Size: 2762 3168
PvuII CAG'CTG
Cuts at: 228 1267 1699 2161 3355 3715 228
Size: 1039 432 462 1194 360 2443
Fragments arranged by size:
2443 1194 1039 462 432 360
RsrII CG'GwC_CG
Cuts at: 4125 4125
Size: 5930
SacI G_AGCT'C
Cuts at: 1401 2081 2944 1401
Size: 680 863 4387
Fragments arranged by size:
4387 863 680
SalI G'TCGA_C
Cuts at: 129 2967 129
Size: 2838 3092
SapI GCTCTTCn'nnn_
Cuts at: 3953 4163 3953
Size: 210 5720
SbfI CC_TGCA'GG
Cuts at: 2977 2977
Size: 5930
ScaI AGT'ACT
Cuts at: 1343 2712 5447 1343
Size: 1369 2735 1826
Fragments arranged by size:
2735 1826 1369
SexAI A'CCwGG_T
Cuts at: 675 675
Size: 5930
SfcI C'TryA_G
Cuts at: 33 473 2012 2973 3658 4651 4842 33
Size: 440 1539 961 685 993 191 1121
Fragments arranged by size:
1539 1121 993 961 685 440 191
SfoI GGC'GCC
Cuts at: 211 1057 3362 3610 211
Size: 846 2305 248 2531
Fragments arranged by size:
2531 2305 846 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5930
SmaI CCC'GGG
Cuts at: 190 805 2952 190
Size: 615 2147 3168
Fragments arranged by size:
3168 2147 615
SmlI C'TyrA_G
Cuts at: 1327 1402 2066 2122 3085 4000 4492 4754 1327
Size: 75 664 56 963 915 492 262 2503
Fragments arranged by size:
2503 963 915 664 492 262 75 56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5930
SphI G_CATG'C
Cuts at: 1594 2983 3483 1594
Size: 1389 500 4041
Fragments arranged by size:
4041 1389 500
StyI C'CwwG_G
Cuts at: 107 715 1383 3064 3418 107
Size: 608 668 1681 354 2619
Fragments arranged by size:
2619 1681 668 608 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3513 3646 4105 4314 3513
Size: 133 459 209 5129
Fragments arranged by size:
5129 459 209 133
TatI w'GTAC_w
Cuts at: 564 1341 2710 5445 564
Size: 777 1369 2735 1049
Fragments arranged by size:
2735 1369 1049 777
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5447 5479 5447
Size: 32 5898
Tth111I GACn'n_nGTC
Cuts at: 677 2428 3727 677
Size: 1751 1299 2880
Fragments arranged by size:
2880 1751 1299
XbaI T'CTAG_A
Cuts at: 2961 2961
Size: 5930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 267 760 1190 3425 267
Size: 493 430 2235 2772
Fragments arranged by size:
2772 2235 493 430
XhoI C'TCGA_G
Cuts at: 2066 2066
Size: 5930
XmaI C'CCGG_G
Cuts at: 188 803 2950 188
Size: 615 2147 3168
Fragments arranged by size:
3168 2147 615
XmnI GAAnn'nnTTC
Cuts at: 2768 2768
Size: 5930
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI AscI BaeI
BcgI BfrBI BmtI BsaXI BsgI BsiWI BsmI BsrGI
BssHII BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PsiI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI