Restriction map for
pF1KA0382
(Circular) (Six-base) MAPSORT of: pF1KA0382.seq Check: 4611 from: 1 to: 7745
pF1KA0382 7745 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 807 3836 807
Size: 3029 4716
AccI GT'mk_AC
Cuts at: 124 4783 124
Size: 4659 3086
Acc65I G'GTAC_C
Cuts at: 4761 4761
Size: 7745
AclI AA'CG_TT
Cuts at: 7585 7585
Size: 7745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 2102 2545 2905 3124 5570 6002 6749 7017
Size: 1497 443 360 219 2446 432 747 268
Cuts at: 7017 605
Size: 1333
Fragments arranged by size:
2446 1497 1333 747 443 432 360 268
219
AfeI AGC'GCT
Cuts at: 4543 4543
Size: 7745
AflIII A'CryG_T
Cuts at: 1657 3224 3329 5097 6201 7178 1657
Size: 1567 105 1768 1104 977 2224
Fragments arranged by size:
2224 1768 1567 1104 977 105
AgeI A'CCGG_T
Cuts at: 6133 6133
Size: 7745
AlwNI CAG_nnn'CTG
Cuts at: 883 961 2080 2194 3460 3622 3894 5050 6617
Size: 78 1119 114 1266 162 272 1156 1567
Cuts at: 6617 883
Size: 2011
Fragments arranged by size:
2011 1567 1266 1156 1119 272 162 114
78
ApaLI G'TGCA_C
Cuts at: 6515 6515
Size: 7745
ApoI r'AATT_y
Cuts at: 728 1058 1797 2570 2597 3141 3870 4525 4749
Size: 330 739 773 27 544 729 655 224
Cuts at: 4749 7098 7354 728
Size: 2349 256 1119
Fragments arranged by size:
2349 1119 773 739 729 655 544 330
256 224 27
AscI GG'CGCG_CC
Cuts at: 1978 1978
Size: 7745
AseI AT'TA_AT
Cuts at: 20 2483 20
Size: 2463 5282
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7745
AvaI C'yCGr_G
Cuts at: 4765 4765
Size: 7745
BamHI G'GATC_C
Cuts at: 4770 4770
Size: 7745
BanI G'GyrC_C
Cuts at: 4137 4761 5175 5423 5458 4137
Size: 624 414 248 35 6424
Fragments arranged by size:
6424 624 414 248 35
BanII G_rGCy'C
Cuts at: 1472 1732 2511 3137 4759 5789 1472
Size: 260 779 626 1622 1030 3428
Fragments arranged by size:
3428 1622 1030 779 626 260
BbeI G_GCGC'C
Cuts at: 5179 5427 5179
Size: 248 7497
BbsI GAAGACnn'nnnn_
Cuts at: 271 375 1467 2099 2869 3245 3941 271
Size: 104 1092 632 770 376 696 4075
Fragments arranged by size:
4075 1092 770 696 632 376 104
BbvCI CC'TCA_GC
Cuts at: 848 848
Size: 7745
BciVI GTATCCnnnnn_n'
Cuts at: 2077 2568 5643 6404 7745 2077
Size: 491 3075 761 1341 2077
Fragments arranged by size:
3075 2077 1341 761 491
BclI T'GATC_A
Cuts at: 2535 2798 5265 2535
Size: 263 2467 5015
Fragments arranged by size:
5015 2467 263
BglI GCCn_nnn'nGGC
Cuts at: 421 421
Size: 7745
BglII A'GATC_T
Cuts at: 1446 3014 5260 7297 1446
Size: 1568 2246 2037 1894
Fragments arranged by size:
2246 2037 1894 1568
BlpI GC'TnA_GC
Cuts at: 4857 4857
Size: 7745
Bme1580I G_kGCm'C
Cuts at: 5370 5463 6519 5370
Size: 93 1056 6596
Fragments arranged by size:
6596 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 982 1007 2452 4369 4957 5038 5373 7517 982
Size: 25 1445 1917 588 81 335 2144 1210
Fragments arranged by size:
2144 1917 1445 1210 588 335 81 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6112 6112
Size: 7745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 511 665 897 983 2216 3081 446
Size: 65 154 232 86 1233 865 5110
Fragments arranged by size:
5110 1233 865 232 154 86 65
Bpu10I CC'TnA_GC
Cuts at: 848 887 1466 1706 1988 3136 848
Size: 39 579 240 282 1148 5457
Fragments arranged by size:
5457 1148 579 282 240 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 4921 5800 6292 6590 1396
Size: 3525 879 492 298 2551
Fragments arranged by size:
3525 2551 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1917 2671 7449 1917
Size: 754 4778 2213
Fragments arranged by size:
4778 2213 754
BsaAI yAC'GTr
Cuts at: 3724 5098 3724
Size: 1374 6371
BsaBI GATnn'nnATC
Cuts at: 6 1487 3657 4280 5259 7296 6
Size: 1481 2170 623 979 2037 455
Fragments arranged by size:
2170 2037 1481 979 623 455
BsaHI Gr'CG_yC
Cuts at: 546 5176 5424 6126 7622 546
Size: 4630 248 702 1496 669
Fragments arranged by size:
4630 1496 702 669 248
BsaWI w'CCGG_w
Cuts at: 4934 5455 6133 6407 6554 6965 4934
Size: 521 678 274 147 411 5714
Fragments arranged by size:
5714 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 887 917 1766 1796 887
Size: 30 849 30 6836
Fragments arranged by size:
6836 849 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 298 623 2290 2752 3613 4414 4469 4578
Size: 52 325 1667 462 861 801 55 109
Cuts at: 4578 246
Size: 3413
Fragments arranged by size:
3413 1667 861 801 462 325 109 55
52
BseYI C'CCAG_C
Cuts at: 1900 4544 5170 5928 6505 7001 1900
Size: 2644 626 758 577 496 2644
Fragments arranged by size:
2644 2644 758 626 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1233 1682 2290 4399 4616 1233
Size: 449 608 2109 217 4362
Fragments arranged by size:
4362 2109 608 449 217
BsiEI CG_ry'CG
Cuts at: 104 5287 5333 6541 104
Size: 5183 46 1208 1308
Fragments arranged by size:
5183 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 686 1472 1732 1808 2511 3137 4247 4759 5537
Size: 786 260 76 703 626 1110 512 778
Cuts at: 5537 5727 6519 686
Size: 190 792 1912
Fragments arranged by size:
1912 1110 792 786 778 703 626 512
260 190 76
Bsp1286I G_dGCh'C
Cuts at: 686 1472 1732 1808 2511 3137 4247 4759 5370
Size: 786 260 76 703 626 1110 512 611
Cuts at: 5370 5463 5537 5727 5789 6519 686
Size: 93 74 190 62 730 1912
Fragments arranged by size:
1912 1110 786 730 703 626 611 512
260 190 93 76 74 62
BspEI T'CCGG_A
Cuts at: 6965 6965
Size: 7745
BspHI T'CATG_A
Cuts at: 623 1935 3171 6921 623
Size: 1312 1236 3750 1447
Fragments arranged by size:
3750 1447 1312 1236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 807 2096 3836 4795 5009 5311 5692 807
Size: 1289 1740 959 214 302 381 2860
Fragments arranged by size:
2860 1740 1289 959 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1653 6037 6091 7575 7740 50
Size: 1603 4384 54 1484 165 55
Fragments arranged by size:
4384 1603 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 221 4070 5657 221
Size: 3849 1587 2309
Fragments arranged by size:
3849 2309 1587
BsrFI r'CCGG_y
Cuts at: 5743 5924 6133 5743
Size: 181 209 7355
Fragments arranged by size:
7355 209 181
BssHII G'CGCG_C
Cuts at: 1978 1978
Size: 7745
BssSI C'ACGA_G
Cuts at: 6016 6374 6016
Size: 358 7387
BstAPI GCAn_nnn'nTGC
Cuts at: 3859 5122 3859
Size: 1263 6482
BstBI TT'CG_AA
Cuts at: 2328 2517 2664 6106 2328
Size: 189 147 3442 3967
Fragments arranged by size:
3967 3442 189 147
BstXI CCAn_nnnn'nTGG
Cuts at: 1908 2411 3188 1908
Size: 503 777 6465
Fragments arranged by size:
6465 777 503
BstYI r'GATC_y
Cuts at: 1 292 821 957 1446 1482 3014 3579 4574
Size: 291 529 136 489 36 1532 565 995
Cuts at: 4574 4770 5237 5260 5595 5841 6853 6939 6951
Size: 196 467 23 335 246 1012 86 12
Cuts at: 6951 7297 1
Size: 346 449
Fragments arranged by size:
1532 1012 995 565 529 489 467 449
346 335 291 246 196 136 86 36
23 12
Bsu36I CC'TnA_GG
Cuts at: 1478 2027 1478
Size: 549 7196
BtgI C'CryG_G
Cuts at: 1918 1961 4090 4117 4208 1918
Size: 43 2129 27 91 5455
Fragments arranged by size:
5455 2129 91 43 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1474 5695 5876 6039 1474
Size: 4221 181 163 3180
Fragments arranged by size:
4221 3180 181 163
BtsI GCAGTG_nn'
Cuts at: 999 4988 999
Size: 3989 3756
ClaI AT'CG_AT
Cuts at: 1401 2690 1401
Size: 1289 6456
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2918 2953 2918
Size: 35 7710
DraI TTT'AAA
Cuts at: 4744 4744
Size: 7745
DraIII CAC_nnn'GTG
Cuts at: 4273 4273
Size: 7745
DrdI GACnn_nn'nnGTC
Cuts at: 379 1931 5451 6309 379
Size: 1552 3520 858 1815
Fragments arranged by size:
3520 1815 1552 858
EaeI y'GGCC_r
Cuts at: 2965 5330 5504 5895 5922 7267 2965
Size: 2365 174 391 27 1345 3443
Fragments arranged by size:
3443 2365 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5330 5330
Size: 7745
EarI CTCTTCn'nnn_
Cuts at: 1884 3125 3916 4310 5768 5978 1884
Size: 1241 791 394 1458 210 3651
Fragments arranged by size:
3651 1458 1241 791 394 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5953 6263 6409 7555 5953
Size: 310 146 1146 6143
Fragments arranged by size:
6143 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1470 1730 2509 3135 4757 1470
Size: 260 779 626 1622 4458
Fragments arranged by size:
4458 1622 779 626 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 511 605 665 897 983 2102 2216 2545
Size: 65 94 60 232 86 1119 114 329
Cuts at: 2545 2905 3081 3124 5570 6002 6749 7017 446
Size: 360 176 43 2446 432 747 268 1174
Fragments arranged by size:
2446 1174 1119 747 432 360 329 268
232 176 114 94 86 65 60 43
EcoNI CCTnn'n_nnAGG
Cuts at: 3557 3557
Size: 7745
EcoO109I rG'GnC_Cy
Cuts at: 1912 2123 3480 4884 1912
Size: 211 1357 1404 4773
Fragments arranged by size:
4773 1404 1357 211
EcoRI G'AATT_C
Cuts at: 2570 4749 2570
Size: 2179 5566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 3262 4243 5764 2638
Size: 624 981 1521 4619
Fragments arranged by size:
4619 1521 981 624
FspI TGC'GCA
Cuts at: 5526 5526
Size: 7745
HaeII r_GCGC'y
Cuts at: 3066 3428 4545 5179 5427 6449 7428 3066
Size: 362 1117 634 248 1022 979 3383
Fragments arranged by size:
3383 1117 1022 979 634 362 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 277 291 309 323 426 458 618
Size: 32 4 14 18 14 103 32 160
Cuts at: 618 650 850 882 1047 1079 1099 1131 1423
Size: 32 200 32 165 32 20 32 292
Cuts at: 1423 1454 1455 1486 1689 1714 1721 1746 2149
Size: 31 1 31 203 25 7 25 403
Cuts at: 2149 2181 2790 2822 4270 4302 4566 4598 4697
Size: 32 609 32 1448 32 264 32 99
Cuts at: 4697 4711 4729 4743 5587 5617 5619 5649 241
Size: 14 18 14 844 30 2 30 2337
Fragments arranged by size:
2337 1448 844 609 403 292 264 203
200 165 160 103 99 32 32 32
32 32 32 32 32 32 32 31
31 30 30 25 25 20 18 18
14 14 14 14 7 4 2 1
HincII GTy'rAC
Cuts at: 3548 4308 4784 3548
Size: 760 476 6509
Fragments arranged by size:
6509 760 476
HindIII A'AGCT_T
Cuts at: 3392 3392
Size: 7745
HpaI GTT'AAC
Cuts at: 4308 4308
Size: 7745
KasI G'GCGC_C
Cuts at: 5175 5423 5175
Size: 248 7497
KpnI G_GTAC'C
Cuts at: 4765 4765
Size: 7745
MfeI C'AATT_G
Cuts at: 1949 1949
Size: 7745
MluI A'CGCG_T
Cuts at: 7178 7178
Size: 7745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1104 1798 2157 2918 3472 3771 6416 6600 1104
Size: 694 359 761 554 299 2645 184 2249
Fragments arranged by size:
2645 2249 761 694 554 359 299 184
MscI TGG'CCA
Cuts at: 2967 5506 2967
Size: 2539 5206
MslI CAynn'nnrTG
Cuts at: 454 1759 2905 3409 3770 4031 4186 4274 4496
Size: 1305 1146 504 361 261 155 88 222
Cuts at: 4496 5734 5861 454
Size: 1238 127 2338
Fragments arranged by size:
2338 1305 1238 1146 504 361 261 222
155 127 88
MspA1I CmG'CkG
Cuts at: 852 1217 2726 3316 4856 5170 5530 6543 6788
Size: 365 1509 590 1540 314 360 1013 245
Cuts at: 6788 852
Size: 1809
Fragments arranged by size:
1809 1540 1509 1013 590 365 360 314
245
NaeI GCC'GGC
Cuts at: 5926 5926
Size: 7745
NarI GG'CG_CC
Cuts at: 5176 5424 5176
Size: 248 7497
NcoI C'CATG_G
Cuts at: 1961 4208 1961
Size: 2247 5498
NdeI CA'TA_TG
Cuts at: 611 4043 611
Size: 3432 4313
NgoMIV G'CCGG_C
Cuts at: 5924 5924
Size: 7745
NspI r_CATG'y
Cuts at: 1661 2755 4798 5298 6205 1661
Size: 1094 2043 500 907 3201
Fragments arranged by size:
3201 2043 1094 907 500
PciI A'CATG_T
Cuts at: 1657 6201 1657
Size: 4544 3201
PflMI CCAn_nnn'nTGG
Cuts at: 4617 4698 4617
Size: 81 7664
PfoI T'CCnGG_A
Cuts at: 960 960
Size: 7745
PmeI GTTT'AAAC
Cuts at: 4744 4744
Size: 7745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2498 2530 3954 3986 6884 6916 2498
Size: 32 1424 32 2898 32 3327
Fragments arranged by size:
3327 2898 1424 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1912 3480 1912
Size: 1568 6177
PshAI GACnn'nnGTC
Cuts at: 612 612
Size: 7745
PstI C_TGCA'G
Cuts at: 1446 3000 4427 4792 5477 1446
Size: 1554 1427 365 685 3714
Fragments arranged by size:
3714 1554 1427 685 365
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7745
PvuII CAG'CTG
Cuts at: 852 1217 3316 5170 5530 852
Size: 365 2099 1854 360 3067
Fragments arranged by size:
3067 2099 1854 365 360
RsrII CG'GwC_CG
Cuts at: 5940 5940
Size: 7745
SacI G_AGCT'C
Cuts at: 1472 1732 2511 3137 4759 1472
Size: 260 779 626 1622 4458
Fragments arranged by size:
4458 1622 779 626 260
SalI G'TCGA_C
Cuts at: 4782 4782
Size: 7745
SanDI GG'GwC_CC
Cuts at: 1912 1912
Size: 7745
SapI GCTCTTCn'nnn_
Cuts at: 3125 3916 5768 5978 3125
Size: 791 1852 210 4892
Fragments arranged by size:
4892 1852 791 210
SbfI CC_TGCA'GG
Cuts at: 4792 4792
Size: 7745
ScaI AGT'ACT
Cuts at: 7262 7262
Size: 7745
SfcI C'TryA_G
Cuts at: 33 766 899 987 1442 1712 1969 2111 2996
Size: 733 133 88 455 270 257 142 885
Cuts at: 2996 3311 4423 4788 5473 6466 6657 33
Size: 315 1112 365 685 993 191 1121
Fragments arranged by size:
1121 1112 993 885 733 685 455 365
315 270 257 191 142 133 88
SfoI GGC'GCC
Cuts at: 5177 5425 5177
Size: 248 7497
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7745
SmaI CCC'GGG
Cuts at: 4767 4767
Size: 7745
SmlI C'TyrA_G
Cuts at: 1375 4900 5815 6307 6569 1375
Size: 3525 915 492 262 2551
Fragments arranged by size:
3525 2551 915 492 262
SpeI A'CTAG_T
Cuts at: 67 673 67
Size: 606 7139
SphI G_CATG'C
Cuts at: 4798 5298 4798
Size: 500 7245
SspI AAT'ATT
Cuts at: 2946 4014 2946
Size: 1068 6677
StuI AGG'CCT
Cuts at: 4021 4021
Size: 7745
StyI C'CwwG_G
Cuts at: 1583 1961 4208 4879 5233 1583
Size: 378 2247 671 354 4095
Fragments arranged by size:
4095 2247 671 378 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1646 2421 4474 5328 5461 5920 6129 1646
Size: 775 2053 854 133 459 209 3262
Fragments arranged by size:
3262 2053 854 775 459 209 133
TatI w'GTAC_w
Cuts at: 429 969 3355 4622 7260 429
Size: 540 2386 1267 2638 914
Fragments arranged by size:
2638 2386 1267 914 540
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 455 487 1766 1798 7262 7294 455
Size: 32 1279 32 5464 32 906
Fragments arranged by size:
5464 1279 906 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 270 3991 5542 270
Size: 3721 1551 2473
Fragments arranged by size:
3721 2473 1551
XbaI T'CTAG_A
Cuts at: 1065 1395 1449 4776 1065
Size: 330 54 3327 4034
Fragments arranged by size:
4034 3327 330 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4699 5240 4699
Size: 541 7204
XmaI C'CCGG_G
Cuts at: 4765 4765
Size: 7745
XmnI GAAnn'nnTTC
Cuts at: 1094 1553 2570 2618 3242 1094
Size: 459 1017 48 624 5597
Fragments arranged by size:
5597 1017 624 459 48
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I EcoRV FseI FspAI NheI NotI
NruI NsiI PacI PasI PmlI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI