Restriction map for
pF1KA0381
(Circular) (Six-base) MAPSORT of: pF1KA0381.seq Check: 6376 from: 1 to: 6317
pF1KA0381 6317 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AatII G_ACGT'C
Cuts at: 1880 1880
Size: 6317
AccI GT'mk_AC
Cuts at: 1032 3355 1032
Size: 2323 3994
Acc65I G'GTAC_C
Cuts at: 905 3333 905
Size: 2428 3889
AclI AA'CG_TT
Cuts at: 6157 6157
Size: 6317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1228 1942 2099 2672 2856 3112 4142 4574
Size: 702 714 157 573 184 256 1030 432
Cuts at: 4574 5321 5589 526
Size: 747 268 1254
Fragments arranged by size:
1254 1030 747 714 702 573 432 268
256 184 157
AflIII A'CryG_T
Cuts at: 455 684 3669 4773 5750 455
Size: 229 2985 1104 977 1022
Fragments arranged by size:
2985 1104 1022 977 229
AgeI A'CCGG_T
Cuts at: 4705 4705
Size: 6317
AhdI GACnn_n'nnGTC
Cuts at: 2601 3186 2601
Size: 585 5732
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1858 1858
Size: 6317
AlwNI CAG_nnn'CTG
Cuts at: 450 873 1308 1596 1660 2382 2713 3270 3622
Size: 423 435 288 64 722 331 557 352
Cuts at: 3622 5189 450
Size: 1567 1578
Fragments arranged by size:
1578 1567 722 557 435 423 352 331
288 64
ApaI G_GGCC'C
Cuts at: 1690 2142 1690
Size: 452 5865
ApaLI G'TGCA_C
Cuts at: 5087 5087
Size: 6317
ApoI r'AATT_y
Cuts at: 582 1015 2407 2974 3321 5670 5926 582
Size: 433 1392 567 347 2349 256 973
Fragments arranged by size:
2349 1392 973 567 433 347 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6317
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
AvaI C'yCGr_G
Cuts at: 1580 1607 1792 2465 3281 3337 1580
Size: 27 185 673 816 56 4560
Fragments arranged by size:
4560 816 673 185 56 27
BamHI G'GATC_C
Cuts at: 2006 3342 2006
Size: 1336 4981
BanI G'GyrC_C
Cuts at: 905 1816 1966 2054 2554 2804 3333 3747 3995
Size: 911 150 88 500 250 529 414 248
Cuts at: 3995 4030 905
Size: 35 3192
Fragments arranged by size:
3192 911 529 500 414 250 248 150
88 35
BanII G_rGCy'C
Cuts at: 251 1690 2074 2142 2822 3331 4361 251
Size: 1439 384 68 680 509 1030 2207
Fragments arranged by size:
2207 1439 1030 680 509 384 68
BbeI G_GCGC'C
Cuts at: 2558 2808 3751 3999 2558
Size: 250 943 248 4876
Fragments arranged by size:
4876 943 250 248
BbsI GAAGACnn'nnnn_
Cuts at: 506 514 1694 2087 3204 506
Size: 8 1180 393 1117 3619
Fragments arranged by size:
3619 1180 1117 393 8
BbvCI CC'TCA_GC
Cuts at: 2426 2426
Size: 6317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1984 2018 3134 3168 1984
Size: 34 1116 34 5133
Fragments arranged by size:
5133 1116 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4215 4976 6317 4215
Size: 761 1341 4215
Fragments arranged by size:
4215 1341 761
BclI T'GATC_A
Cuts at: 2642 3837 2642
Size: 1195 5122
BglI GCCn_nnn'nGGC
Cuts at: 134 134
Size: 6317
BglII A'GATC_T
Cuts at: 344 3832 5869 344
Size: 3488 2037 792
Fragments arranged by size:
3488 2037 792
BlpI GC'TnA_GC
Cuts at: 3429 3429
Size: 6317
Bme1580I G_kGCm'C
Cuts at: 684 1690 1821 2142 3942 4035 5091 684
Size: 1006 131 321 1800 93 1056 1910
Fragments arranged by size:
1910 1800 1056 1006 321 131 93
BmrI ACTGGGnnnn_n'
Cuts at: 1944 2816 3529 3610 3945 6089 1944
Size: 872 713 81 335 2144 2172
Fragments arranged by size:
2172 2144 872 713 335 81
BmtI G_CTAG'C
Cuts at: 1135 1135
Size: 6317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2088 4684 2088
Size: 2596 3721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 580 787 989 995 1009 1147 1306 1321
Size: 373 207 202 6 14 138 159 15
Cuts at: 1321 1513 2266 2314 2674 2758 2812 2908 3178
Size: 192 753 48 360 84 54 96 270
Cuts at: 3178 207
Size: 3346
Fragments arranged by size:
3346 753 373 360 270 207 202 192
159 138 96 84 54 48 15 14
6
Bpu10I CC'TnA_GC
Cuts at: 2234 2426 2234
Size: 192 6125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2242 3220 3493 4372 4864 5162 2242
Size: 978 273 879 492 298 3397
Fragments arranged by size:
3397 978 879 492 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 1436 6021 1436
Size: 4585 1732
BsaAI yAC'GTr
Cuts at: 3670 3670
Size: 6317
BsaBI GATnn'nnATC
Cuts at: 6 2647 3831 5868 6
Size: 2641 1184 2037 455
Fragments arranged by size:
2641 2037 1184 455
BsaHI Gr'CG_yC
Cuts at: 1877 2555 2805 3748 3996 4698 6194 1877
Size: 678 250 943 248 702 1496 2000
Fragments arranged by size:
2000 1496 943 702 678 250 248
BsaWI w'CCGG_w
Cuts at: 518 590 1460 3506 4027 4705 4979 5126 5537
Size: 72 870 2046 521 678 274 147 411
Cuts at: 5537 518
Size: 1298
Fragments arranged by size:
2046 1298 870 678 521 411 274 147
72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2779 2809 2779
Size: 30 6287
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 259 481 484 1730 1733 1966 2200 2245
Size: 121 222 3 1246 3 233 234 45
Cuts at: 2245 2311 3057 3060 3070 3073 3076 3175 3182
Size: 66 746 3 10 3 3 99 7
Cuts at: 3182 138
Size: 3273
Fragments arranged by size:
3273 1246 746 234 233 222 121 99
66 45 10 7 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 1026 1634 1867 1904 2067 3742 4500 5077 5573
Size: 608 233 37 163 1675 758 577 496
Cuts at: 5573 1026
Size: 1770
Fragments arranged by size:
1770 1675 758 608 577 496 233 163
37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1231 814
Size: 417 5900
BsiEI CG_ry'CG
Cuts at: 104 420 3859 3905 5113 104
Size: 316 3439 46 1208 1308
Fragments arranged by size:
3439 1308 1208 316 46
BsiHKAI G_wGCw'C
Cuts at: 251 2303 3331 4109 4299 5091 251
Size: 2052 1028 778 190 792 1477
Fragments arranged by size:
2052 1477 1028 792 778 190
BsmI GAATG_Cn'
Cuts at: 1516 1516
Size: 6317
BsmBI CGTCTCn'nnnn_
Cuts at: 466 466
Size: 6317
Bsp1286I G_dGCh'C
Cuts at: 251 684 1690 1821 2074 2142 2303 2822 3331
Size: 433 1006 131 253 68 161 519 509
Cuts at: 3331 3942 4035 4109 4299 4361 5091 251
Size: 611 93 74 190 62 730 1477
Fragments arranged by size:
1477 1006 730 611 519 509 433 253
190 161 131 93 74 68 62
BspEI T'CCGG_A
Cuts at: 518 590 1460 5537 518
Size: 72 870 4077 1298
Fragments arranged by size:
4077 1298 870 72
BspHI T'CATG_A
Cuts at: 2531 2645 2843 5493 2531
Size: 114 198 2650 3355
Fragments arranged by size:
3355 2650 198 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1733 1986 3367 3581 3883 4264 1733
Size: 253 1381 214 302 381 3786
Fragments arranged by size:
3786 1381 381 302 253 214
BsrBI CCG'CTC
Cuts at: 50 1347 1506 2319 2418 2776 3066 3114 3288
Size: 1297 159 813 99 358 290 48 174
Cuts at: 3288 4609 4663 6147 6312 50
Size: 1321 54 1484 165 55
Fragments arranged by size:
1484 1321 1297 813 358 290 174 165
159 99 55 54 48
BsrDI GCAATG_nn'
Cuts at: 4229 4229
Size: 6317
BsrFI r'CCGG_y
Cuts at: 902 3299 4315 4496 4705 902
Size: 2397 1016 181 209 2514
Fragments arranged by size:
2514 2397 1016 209 181
BssHII G'CGCG_C
Cuts at: 3073 3073
Size: 6317
BssSI C'ACGA_G
Cuts at: 790 4588 4946 790
Size: 3798 358 2161
Fragments arranged by size:
3798 2161 358
BstAPI GCAn_nnn'nTGC
Cuts at: 825 2382 3694 825
Size: 1557 1312 3448
Fragments arranged by size:
3448 1557 1312
BstBI TT'CG_AA
Cuts at: 4678 4678
Size: 6317
BstXI CCAn_nnnn'nTGG
Cuts at: 387 2457 387
Size: 2070 4247
BstYI r'GATC_y
Cuts at: 1 285 344 770 2006 2189 3027 3342 3809
Size: 284 59 426 1236 183 838 315 467
Cuts at: 3809 3832 4167 4413 5425 5511 5523 5869 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1236 1012 838 467 449 426 346 335
315 284 246 183 86 59 23 12
BstZ17I GTA'TAC
Cuts at: 1033 1033
Size: 6317
Bsu36I CC'TnA_GG
Cuts at: 701 1853 2758 701
Size: 1152 905 4260
Fragments arranged by size:
4260 1152 905
BtgI C'CryG_G
Cuts at: 107 132 431 107
Size: 25 299 5993
Fragments arranged by size:
5993 299 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 454 4267 4448 4611 454
Size: 3813 181 163 2160
Fragments arranged by size:
3813 2160 181 163
BtsI GCAGTG_nn'
Cuts at: 171 319 1251 1303 1878 3560 171
Size: 148 932 52 575 1682 2928
Fragments arranged by size:
2928 1682 932 575 148 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1679 1714 1679
Size: 35 6282
DraI TTT'AAA
Cuts at: 3316 3316
Size: 6317
DrdI GACnn_nn'nnGTC
Cuts at: 2833 4023 4881 2833
Size: 1190 858 4269
Fragments arranged by size:
4269 1190 858
EaeI y'GGCC_r
Cuts at: 801 2130 2573 3902 4076 4467 4494 5839 801
Size: 1329 443 1329 174 391 27 1345 1279
Fragments arranged by size:
1345 1329 1329 1279 443 391 174 27
EagI C'GGCC_G
Cuts at: 3902 3902
Size: 6317
EarI CTCTTCn'nnn_
Cuts at: 1532 2269 2398 4340 4550 1532
Size: 737 129 1942 210 3299
Fragments arranged by size:
3299 1942 737 210 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 985 4525 4835 4981 6127 985
Size: 3540 310 146 1146 1175
Fragments arranged by size:
3540 1175 1146 310 146
EcoICRI GAG'CTC
Cuts at: 249 3329 249
Size: 3080 3237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 526 580 787 989 995 1009 1147 1228
Size: 319 54 207 202 6 14 138 81
Cuts at: 1228 1306 1321 1513 1942 2099 2266 2314 2672
Size: 78 15 192 429 157 167 48 358
Cuts at: 2672 2674 2758 2812 2856 2908 3112 3178 4142
Size: 2 84 54 44 52 204 66 964
Cuts at: 4142 4574 5321 5589 207
Size: 432 747 268 935
Fragments arranged by size:
964 935 747 432 429 358 319 268
207 204 202 192 167 157 138 84
81 78 66 54 54 52 48 44
15 14 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 1852 2049 2233 2293 1852
Size: 197 184 60 5876
Fragments arranged by size:
5876 197 184 60
EcoO109I rG'GnC_Cy
Cuts at: 375 1657 1686 1798 1857 2138 2226 2766 3135
Size: 1282 29 112 59 281 88 540 369
Cuts at: 3135 3267 3456 375
Size: 132 189 3236
Fragments arranged by size:
3236 1282 540 369 281 189 132 112
88 59 29
EcoRI G'AATT_C
Cuts at: 1015 2974 3321 1015
Size: 1959 347 4011
Fragments arranged by size:
4011 1959 347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4336 4336
Size: 6317
FspI TGC'GCA
Cuts at: 4098 4098
Size: 6317
HaeII r_GCGC'y
Cuts at: 2558 2808 3751 3999 5021 6000 2558
Size: 250 943 248 1022 979 2875
Fragments arranged by size:
2875 1022 979 943 250 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 432 464 2587 2619 3172 3204 4159 4189 4191
Size: 32 2123 32 553 32 955 30 2
Cuts at: 4191 4221 432
Size: 30 2528
Fragments arranged by size:
2528 2123 955 553 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1404 2724 3356 1404
Size: 1320 632 4365
Fragments arranged by size:
4365 1320 632
HindIII A'AGCT_T
Cuts at: 2173 2173
Size: 6317
KasI G'GCGC_C
Cuts at: 2554 2804 3747 3995 2554
Size: 250 943 248 4876
Fragments arranged by size:
4876 943 250 248
KpnI G_GTAC'C
Cuts at: 909 3337 909
Size: 2428 3889
MluI A'CGCG_T
Cuts at: 455 5750 455
Size: 5295 1022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4988 5172 4988
Size: 184 6133
MscI TGG'CCA
Cuts at: 803 2575 4078 803
Size: 1772 1503 3042
Fragments arranged by size:
3042 1772 1503
MslI CAynn'nnrTG
Cuts at: 1067 2306 2860 4306 4433 1067
Size: 1239 554 1446 127 2951
Fragments arranged by size:
2951 1446 1239 554 127
MspA1I CmG'CkG
Cuts at: 398 3123 3129 3428 3742 4102 5115 5360 398
Size: 2725 6 299 314 360 1013 245 1355
Fragments arranged by size:
2725 1355 1013 360 314 299 245 6
NaeI GCC'GGC
Cuts at: 4498 4498
Size: 6317
NarI GG'CG_CC
Cuts at: 2555 2805 3748 3996 2555
Size: 250 943 248 4876
Fragments arranged by size:
4876 943 250 248
NcoI C'CATG_G
Cuts at: 107 132 431 107
Size: 25 299 5993
Fragments arranged by size:
5993 299 25
NgoMIV G'CCGG_C
Cuts at: 4496 4496
Size: 6317
NheI G'CTAG_C
Cuts at: 1131 1131
Size: 6317
NspI r_CATG'y
Cuts at: 688 1410 1995 2244 2685 3370 3870 4777 688
Size: 722 585 249 441 685 500 907 2228
Fragments arranged by size:
2228 907 722 685 585 500 441 249
PasI CC'CwG_GG
Cuts at: 672 1681 672
Size: 1009 5308
PciI A'CATG_T
Cuts at: 684 4773 684
Size: 4089 2228
PflMI CCAn_nnn'nTGG
Cuts at: 138 495 1681 3270 138
Size: 357 1186 1589 3185
Fragments arranged by size:
3185 1589 1186 357
PfoI T'CCnGG_A
Cuts at: 188 1076 1307 1337 2009 2291 2411 2651 188
Size: 888 231 30 672 282 120 240 3854
Fragments arranged by size:
3854 888 672 282 240 231 120 30
PmeI GTTT'AAAC
Cuts at: 3316 3316
Size: 6317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5456 5488 5456
Size: 32 6285
PpuMI rG'GwC_Cy
Cuts at: 375 1798 1857 2226 3135 375
Size: 1423 59 369 909 3557
Fragments arranged by size:
3557 1423 909 369 59
PshAI GACnn'nnGTC
Cuts at: 893 893
Size: 6317
PspOMI G'GGCC_C
Cuts at: 1686 2138 1686
Size: 452 5865
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1270 1302 1270
Size: 32 6285
PstI C_TGCA'G
Cuts at: 209 355 821 1262 3147 3364 4049 209
Size: 146 466 441 1885 217 685 2477
Fragments arranged by size:
2477 1885 685 466 441 217 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6317
PvuII CAG'CTG
Cuts at: 398 3742 4102 398
Size: 3344 360 2613
Fragments arranged by size:
3344 2613 360
RsrII CG'GwC_CG
Cuts at: 4512 4512
Size: 6317
SacI G_AGCT'C
Cuts at: 251 3331 251
Size: 3080 3237
SalI G'TCGA_C
Cuts at: 3354 3354
Size: 6317
SanDI GG'GwC_CC
Cuts at: 1798 1798
Size: 6317
SapI GCTCTTCn'nnn_
Cuts at: 4340 4550 4340
Size: 210 6107
SbfI CC_TGCA'GG
Cuts at: 3364 3364
Size: 6317
ScaI AGT'ACT
Cuts at: 5834 5834
Size: 6317
SexAI A'CCwGG_T
Cuts at: 836 3182 836
Size: 2346 3971
SfcI C'TryA_G
Cuts at: 33 205 351 817 1258 3143 3360 4045 5038
Size: 172 146 466 441 1885 217 685 993
Cuts at: 5038 5229 33
Size: 191 1121
Fragments arranged by size:
1885 1121 993 685 466 441 217 191
172 146
SfoI GGC'GCC
Cuts at: 2556 2806 3749 3997 2556
Size: 250 943 248 4876
Fragments arranged by size:
4876 943 250 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6317
SmaI CCC'GGG
Cuts at: 1582 1794 2467 3283 3339 1582
Size: 212 673 816 56 4560
Fragments arranged by size:
4560 816 673 212 56
SmlI C'TyrA_G
Cuts at: 2257 3235 3472 4387 4879 5141 2257
Size: 978 237 915 492 262 3433
Fragments arranged by size:
3433 978 915 492 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6317
SphI G_CATG'C
Cuts at: 3370 3870 3370
Size: 500 5817
StuI AGG'CCT
Cuts at: 1222 2929 3007 1222
Size: 1707 78 4532
Fragments arranged by size:
4532 1707 78
StyI C'CwwG_G
Cuts at: 107 132 431 752 1373 2924 3451 3805 107
Size: 25 299 321 621 1551 527 354 2619
Fragments arranged by size:
2619 1551 621 527 354 321 299 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 766 905 1926 1931 2109 3900 4033 4492 4701
Size: 139 1021 5 178 1791 133 459 209
Cuts at: 4701 766
Size: 2382
Fragments arranged by size:
2382 1791 1021 459 209 178 139 133
5
TatI w'GTAC_w
Cuts at: 1211 5832 1211
Size: 4621 1696
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1881 1913 2313 2345 5834 5866 1881
Size: 32 400 32 3489 32 2332
Fragments arranged by size:
3489 2332 400 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1150 4114 1150
Size: 2964 3353
XbaI T'CTAG_A
Cuts at: 3030 3348 3030
Size: 318 5999
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2208 3812 2208
Size: 1604 4713
XmaI C'CCGG_G
Cuts at: 1580 1792 2465 3281 3337 1580
Size: 212 673 816 56 4560
Fragments arranged by size:
4560 816 673 212 56
XmnI GAAnn'nnTTC
Cuts at: 1927 2407 1927
Size: 480 5837
ZraI GAC'GTC
Cuts at: 1878 1878
Size: 6317
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AloI AscI AvrII BaeI
BfrBI BmgBI BsiWI BsrGI BstEII ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NotI NruI NsiI
PacI PmlI PsiI PspXI SacII SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI