Restriction map for
pF1KA0377
(Circular) (Six-base) MAPSORT of: pF1KA0377.seq Check: 8924 from: 1 to: 7331
pF1KA0377 7331 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 889 2204 4369 889
Size: 1315 2165 3851
Fragments arranged by size:
3851 2165 1315
Acc65I G'GTAC_C
Cuts at: 4347 4347
Size: 7331
AclI AA'CG_TT
Cuts at: 7171 7171
Size: 7331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 1546 1690 2805 3591 3662 4163 5156 5588
Size: 608 144 1115 786 71 501 993 432
Cuts at: 5588 6335 6603 938
Size: 747 268 1666
Fragments arranged by size:
1666 1115 993 786 747 608 501 432
268 144 71
AfeI AGC'GCT
Cuts at: 3385 3385
Size: 7331
AflIII A'CryG_T
Cuts at: 2599 3633 4683 5787 6764 2599
Size: 1034 1050 1104 977 3166
Fragments arranged by size:
3166 1104 1050 1034 977
AgeI A'CCGG_T
Cuts at: 5719 5719
Size: 7331
AhdI GACnn_n'nnGTC
Cuts at: 2196 2196
Size: 7331
AlwNI CAG_nnn'CTG
Cuts at: 4636 6203 4636
Size: 1567 5764
ApaI G_GGCC'C
Cuts at: 3007 3007
Size: 7331
ApaLI G'TGCA_C
Cuts at: 6101 6101
Size: 7331
ApoI r'AATT_y
Cuts at: 3235 3627 4335 6684 6940 3235
Size: 392 708 2349 256 3626
Fragments arranged by size:
3626 2349 708 392 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7331
AvaI C'yCGr_G
Cuts at: 2564 3084 4351 2564
Size: 520 1267 5544
Fragments arranged by size:
5544 1267 520
AvrII C'CTAG_G
Cuts at: 3150 3150
Size: 7331
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1657 1690 1657
Size: 33 7298
BamHI G'GATC_C
Cuts at: 557 2781 4356 557
Size: 2224 1575 3532
Fragments arranged by size:
3532 2224 1575
BanI G'GyrC_C
Cuts at: 184 3174 3750 4347 4761 5009 5044 184
Size: 2990 576 597 414 248 35 2471
Fragments arranged by size:
2990 2471 597 576 414 248 35
BanII G_rGCy'C
Cuts at: 230 3007 3449 3701 4345 5375 230
Size: 2777 442 252 644 1030 2186
Fragments arranged by size:
2777 2186 1030 644 442 252
BbeI G_GCGC'C
Cuts at: 4765 5013 4765
Size: 248 7083
BbsI GAAGACnn'nnnn_
Cuts at: 656 722 1510 2473 2640 656
Size: 66 788 963 167 5347
Fragments arranged by size:
5347 963 788 167 66
BbvCI CC'TCA_GC
Cuts at: 1232 1712 1232
Size: 480 6851
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1135 1169 1135
Size: 34 7297
BciVI GTATCCnnnnn_n'
Cuts at: 5229 5990 7331 5229
Size: 761 1341 5229
Fragments arranged by size:
5229 1341 761
BclI T'GATC_A
Cuts at: 2133 3612 4851 2133
Size: 1479 1239 4613
Fragments arranged by size:
4613 1479 1239
BglI GCCn_nnn'nGGC
Cuts at: 3428 3428
Size: 7331
BglII A'GATC_T
Cuts at: 1826 4846 6883 1826
Size: 3020 2037 2274
Fragments arranged by size:
3020 2274 2037
BlpI GC'TnA_GC
Cuts at: 747 3850 4443 747
Size: 3103 593 3635
Fragments arranged by size:
3635 3103 593
Bme1580I G_kGCm'C
Cuts at: 187 2862 3007 4956 5049 6105 187
Size: 2675 145 1949 93 1056 1413
Fragments arranged by size:
2675 1949 1413 1056 145 93
BmrI ACTGGGnnnn_n'
Cuts at: 4543 4624 4959 7103 4543
Size: 81 335 2144 4771
Fragments arranged by size:
4771 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1658 2590 5698 1658
Size: 932 3108 3291
Fragments arranged by size:
3291 3108 932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 773 917 1414 1492 1558 1769 2284 3361
Size: 585 144 497 78 66 211 515 1077
Cuts at: 3361 3652 4033 188
Size: 291 381 3486
Fragments arranged by size:
3486 1077 585 515 497 381 291 211
144 78 66
Bpu10I CC'TnA_GC
Cuts at: 1232 1421 1712 1232
Size: 189 291 6851
Fragments arranged by size:
6851 291 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1396 1807 1998 3261 3441 4507 5386 5878
Size: 1143 411 191 1263 180 1066 879 492
Cuts at: 5878 6176 253
Size: 298 1408
Fragments arranged by size:
1408 1263 1143 1066 879 492 411 298
191 180
BsaI GGTCTCn'nnnn_
Cuts at: 2668 3667 4056 7035 2668
Size: 999 389 2979 2964
Fragments arranged by size:
2979 2964 999 389
BsaAI yAC'GTr
Cuts at: 3349 3636 4684 3349
Size: 287 1048 5996
Fragments arranged by size:
5996 1048 287
BsaBI GATnn'nnATC
Cuts at: 6 2752 4845 6882 6
Size: 2746 2093 2037 455
Fragments arranged by size:
2746 2093 2037 455
BsaHI Gr'CG_yC
Cuts at: 4762 5010 5712 7208 4762
Size: 248 702 1496 4885
Fragments arranged by size:
4885 1496 702 248
BsaWI w'CCGG_w
Cuts at: 554 1568 4520 5041 5719 5993 6140 6551 554
Size: 1014 2952 521 678 274 147 411 1334
Fragments arranged by size:
2952 1334 1014 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 368 398 2923 2953 368
Size: 30 2525 30 4746
Fragments arranged by size:
4746 2525 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 770 865 1513 1729 2464 2917 3033 3796
Size: 514 95 648 216 735 453 116 763
Cuts at: 3796 4039 4171 4297 4330 256
Size: 243 132 126 33 3257
Fragments arranged by size:
3257 763 735 648 514 453 243 216
132 126 116 95 33
BseYI C'CCAG_C
Cuts at: 180 742 1719 2990 3490 3518 4137 4756 5514
Size: 562 977 1271 500 28 619 619 758
Cuts at: 5514 6091 6587 180
Size: 577 496 924
Fragments arranged by size:
1271 977 924 758 619 619 577 562
500 496 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 1996 2365 731
Size: 1265 369 5697
Fragments arranged by size:
5697 1265 369
BsiEI CG_ry'CG
Cuts at: 104 4873 4919 6127 104
Size: 4769 46 1208 1308
Fragments arranged by size:
4769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 230 602 2576 3449 3693 3701 4345 5123 5313
Size: 372 1974 873 244 8 644 778 190
Cuts at: 5313 6105 230
Size: 792 1456
Fragments arranged by size:
1974 1456 873 792 778 644 372 244
190 8
BsmI GAATG_Cn'
Cuts at: 634 2189 634
Size: 1555 5776
BsmBI CGTCTCn'nnnn_
Cuts at: 775 1801 2398 2608 775
Size: 1026 597 210 5498
Fragments arranged by size:
5498 1026 597 210
Bsp1286I G_dGCh'C
Cuts at: 187 230 602 2576 2862 3007 3449 3693 3701
Size: 43 372 1974 286 145 442 244 8
Cuts at: 3701 4345 4956 5049 5123 5313 5375 6105 187
Size: 644 611 93 74 190 62 730 1413
Fragments arranged by size:
1974 1413 730 644 611 442 372 286
244 190 145 93 74 62 43 8
BspEI T'CCGG_A
Cuts at: 6551 6551
Size: 7331
BspHI T'CATG_A
Cuts at: 6507 6507
Size: 7331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2216 2519 4381 4595 4897 5278 2216
Size: 303 1862 214 302 381 4269
Fragments arranged by size:
4269 1862 381 303 302 214
BsrBI CCG'CTC
Cuts at: 50 5623 5677 7161 7326 50
Size: 5573 54 1484 165 55
Fragments arranged by size:
5573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1289 2974 3895 5243 1289
Size: 1685 921 1348 3377
Fragments arranged by size:
3377 1685 1348 921
BsrFI r'CCGG_y
Cuts at: 3101 5329 5510 5719 3101
Size: 2228 181 209 4713
Fragments arranged by size:
4713 2228 209 181
BssSI C'ACGA_G
Cuts at: 2524 5602 5960 2524
Size: 3078 358 3895
Fragments arranged by size:
3895 3078 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4708 4708
Size: 7331
BstBI TT'CG_AA
Cuts at: 489 2595 5692 489
Size: 2106 3097 2128
Fragments arranged by size:
3097 2128 2106
BstEII G'GTnAC_C
Cuts at: 3913 3913
Size: 7331
BstXI CCAn_nnnn'nTGG
Cuts at: 1164 3490 3910 1164
Size: 2326 420 4585
Fragments arranged by size:
4585 2326 420
BstYI r'GATC_y
Cuts at: 1 557 1626 1826 2171 2309 2781 4148 4356
Size: 556 1069 200 345 138 472 1367 208
Cuts at: 4356 4823 4846 5181 5427 6439 6525 6537 6883
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6883 1
Size: 449
Fragments arranged by size:
1367 1069 1012 556 472 467 449 346
345 335 246 208 200 138 86 23
12
BstZ17I GTA'TAC
Cuts at: 890 890
Size: 7331
Bsu36I CC'TnA_GG
Cuts at: 623 2048 2999 4154 4280 4313 623
Size: 1425 951 1155 126 33 3641
Fragments arranged by size:
3641 1425 1155 951 126 33
BtgI C'CryG_G
Cuts at: 143 189 1004 1210 1223 3056 3170 143
Size: 46 815 206 13 1833 114 4304
Fragments arranged by size:
4304 1833 815 206 114 46 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1979 5281 5462 5625 1979
Size: 3302 181 163 3685
Fragments arranged by size:
3685 3302 181 163
BtsI GCAGTG_nn'
Cuts at: 423 804 2862 3199 3537 4574 423
Size: 381 2058 337 338 1037 3180
Fragments arranged by size:
3180 2058 1037 381 338 337
DraI TTT'AAA
Cuts at: 4330 4330
Size: 7331
DraIII CAC_nnn'GTG
Cuts at: 1998 1998
Size: 7331
DrdI GACnn_nn'nnGTC
Cuts at: 5037 5895 5037
Size: 858 6473
EaeI y'GGCC_r
Cuts at: 122 412 515 1019 1698 3121 3429 4916 5090
Size: 290 103 504 679 1423 308 1487 174
Cuts at: 5090 5481 5508 6853 122
Size: 391 27 1345 600
Fragments arranged by size:
1487 1423 1345 679 600 504 391 308
290 174 103 27
EagI C'GGCC_G
Cuts at: 4916 4916
Size: 7331
EarI CTCTTCn'nnn_
Cuts at: 206 563 1927 2278 2895 3359 4139 5354 5564
Size: 357 1364 351 617 464 780 1215 210
Cuts at: 5564 206
Size: 1973
Fragments arranged by size:
1973 1364 1215 780 617 464 357 351
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1801 5539 5849 5995 7141 1801
Size: 3738 310 146 1146 1991
Fragments arranged by size:
3738 1991 1146 310 146
EcoICRI GAG'CTC
Cuts at: 228 3447 3699 4343 228
Size: 3219 252 644 3216
Fragments arranged by size:
3219 3216 644 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 773 917 938 1414 1492 1546 1558 1690
Size: 585 144 21 476 78 54 12 132
Cuts at: 1690 1769 2284 2805 3361 3591 3652 3662 4033
Size: 79 515 521 556 230 61 10 371
Cuts at: 4033 4163 5156 5588 6335 6603 188
Size: 130 993 432 747 268 916
Fragments arranged by size:
993 916 747 585 556 521 515 476
432 371 268 230 144 132 130 79
78 61 54 21 12 10
EcoO109I rG'GnC_Cy
Cuts at: 1881 2190 3003 3004 3807 3870 3933 4308 4470
Size: 309 813 1 803 63 63 375 162
Cuts at: 4470 1881
Size: 4742
Fragments arranged by size:
4742 813 803 375 309 162 63 63
1
EcoRI G'AATT_C
Cuts at: 3235 4335 3235
Size: 1100 6231
EcoRV GAT'ATC
Cuts at: 985 1446 2301 985
Size: 461 855 6015
Fragments arranged by size:
6015 855 461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5350 5350
Size: 7331
FspI TGC'GCA
Cuts at: 5112 5112
Size: 7331
HaeII r_GCGC'y
Cuts at: 2295 3261 3387 3850 4765 5013 6035 7014 2295
Size: 966 126 463 915 248 1022 979 2612
Fragments arranged by size:
2612 1022 979 966 915 463 248 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 969 974 1001 1006 1533 1565 2182 2214 2518
Size: 5 27 5 527 32 617 32 304
Cuts at: 2518 2550 2773 2805 3014 3046 3064 3096 3577
Size: 32 223 32 209 32 18 32 481
Cuts at: 3577 3604 3609 3636 4249 4281 5173 5203 5205
Size: 27 5 27 613 32 892 30 2
Cuts at: 5205 5235 969
Size: 30 3065
Fragments arranged by size:
3065 892 617 613 527 481 304 223
209 32 32 32 32 32 32 32
30 30 27 27 27 18 5 5
5 2
HincII GTy'rAC
Cuts at: 2541 4370 2541
Size: 1829 5502
HindIII A'AGCT_T
Cuts at: 484 1607 484
Size: 1123 6208
KasI G'GCGC_C
Cuts at: 4761 5009 4761
Size: 248 7083
KpnI G_GTAC'C
Cuts at: 4351 4351
Size: 7331
MfeI C'AATT_G
Cuts at: 1579 1579
Size: 7331
MluI A'CGCG_T
Cuts at: 2599 6764 2599
Size: 4165 3166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2233 6002 6186 2233
Size: 3769 184 3378
Fragments arranged by size:
3769 3378 184
MscI TGG'CCA
Cuts at: 414 517 1021 3431 5092 414
Size: 103 504 2410 1661 2653
Fragments arranged by size:
2653 2410 1661 504 103
MslI CAynn'nnrTG
Cuts at: 1177 1270 1918 2528 3133 3266 3908 5320 5447
Size: 93 648 610 605 133 642 1412 127
Cuts at: 5447 1177
Size: 3061
Fragments arranged by size:
3061 1412 648 642 610 605 133 127
93
MspA1I CmG'CkG
Cuts at: 751 1524 1985 2064 4141 4442 4756 5116 6129
Size: 773 461 79 2077 301 314 360 1013
Cuts at: 6129 6374 751
Size: 245 1708
Fragments arranged by size:
2077 1708 1013 773 461 360 314 301
245 79
NaeI GCC'GGC
Cuts at: 5512 5512
Size: 7331
NarI GG'CG_CC
Cuts at: 4762 5010 4762
Size: 248 7083
NcoI C'CATG_G
Cuts at: 1004 1210 3056 3170 1004
Size: 206 1846 114 5165
Fragments arranged by size:
5165 1846 206 114
NgoMIV G'CCGG_C
Cuts at: 5510 5510
Size: 7331
NspI r_CATG'y
Cuts at: 1743 3926 4384 4884 5791 1743
Size: 2183 458 500 907 3283
Fragments arranged by size:
3283 2183 907 500 458
PasI CC'CwG_GG
Cuts at: 3679 3679
Size: 7331
PciI A'CATG_T
Cuts at: 5787 5787
Size: 7331
PflMI CCAn_nnn'nTGG
Cuts at: 868 868
Size: 7331
PfoI T'CCnGG_A
Cuts at: 1430 2174 2943 3668 4109 1430
Size: 744 769 725 441 4652
Fragments arranged by size:
4652 769 744 725 441
PmeI GTTT'AAAC
Cuts at: 4330 4330
Size: 7331
PmlI CAC'GTG
Cuts at: 3636 3636
Size: 7331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2817 6470 6502 2785
Size: 32 3653 32 3614
Fragments arranged by size:
3653 3614 32 32
PpuMI rG'GwC_Cy
Cuts at: 2190 3807 3870 3933 4308 2190
Size: 1617 63 63 375 5213
Fragments arranged by size:
5213 1617 375 63 63
PshAI GACnn'nnGTC
Cuts at: 364 364
Size: 7331
PspOMI G'GGCC_C
Cuts at: 3003 3003
Size: 7331
PspXI vC'TCGA_Gb
Cuts at: 3084 3084
Size: 7331
PstI C_TGCA'G
Cuts at: 346 566 2213 2516 3291 4298 4378 5063 346
Size: 220 1647 303 775 1007 80 685 2614
Fragments arranged by size:
2614 1647 1007 775 685 303 220 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7331
PvuII CAG'CTG
Cuts at: 751 1524 1985 2064 4141 4756 5116 751
Size: 773 461 79 2077 615 360 2966
Fragments arranged by size:
2966 2077 773 615 461 360 79
RsrII CG'GwC_CG
Cuts at: 5526 5526
Size: 7331
SacI G_AGCT'C
Cuts at: 230 3449 3701 4345 230
Size: 3219 252 644 3216
Fragments arranged by size:
3219 3216 644 252
SalI G'TCGA_C
Cuts at: 4368 4368
Size: 7331
SapI GCTCTTCn'nnn_
Cuts at: 5354 5564 5354
Size: 210 7121
SbfI CC_TGCA'GG
Cuts at: 566 4378 566
Size: 3812 3519
ScaI AGT'ACT
Cuts at: 2082 6848 2082
Size: 4766 2565
SexAI A'CCwGG_T
Cuts at: 1482 1482
Size: 7331
SfcI C'TryA_G
Cuts at: 33 342 562 2023 2201 2209 2245 2512 3287
Size: 309 220 1461 178 8 36 267 775
Cuts at: 3287 3578 4294 4374 5059 6052 6243 33
Size: 291 716 80 685 993 191 1121
Fragments arranged by size:
1461 1121 993 775 716 685 309 291
267 220 191 178 80 36 8
SfoI GGC'GCC
Cuts at: 4763 5011 4763
Size: 248 7083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7331
SmaI CCC'GGG
Cuts at: 4353 4353
Size: 7331
SmlI C'TyrA_G
Cuts at: 232 1411 1822 1977 3084 3240 3456 4486 5401
Size: 1179 411 155 1107 156 216 1030 915
Cuts at: 5401 5893 6155 232
Size: 492 262 1408
Fragments arranged by size:
1408 1179 1107 1030 915 492 411 262
216 156 155
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7331
SphI G_CATG'C
Cuts at: 4384 4884 4384
Size: 500 6831
StuI AGG'CCT
Cuts at: 3090 3322 3656 3090
Size: 232 334 6765
Fragments arranged by size:
6765 334 232
StyI C'CwwG_G
Cuts at: 159 1004 1210 1354 1877 2919 3056 3150 3170
Size: 845 206 144 523 1042 137 94 20
Cuts at: 3170 3400 3657 4465 4819 159
Size: 230 257 808 354 2671
Fragments arranged by size:
2671 1042 845 808 523 354 257 230
206 144 137 94 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1367 1926 2179 3141 3532 4914 5047 5506 5715
Size: 559 253 962 391 1382 133 459 209
Cuts at: 5715 1367
Size: 2983
Fragments arranged by size:
2983 1382 962 559 459 391 253 209
133
TatI w'GTAC_w
Cuts at: 1533 2080 2558 6846 1533
Size: 547 478 4288 2018
Fragments arranged by size:
4288 2018 547 478
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2766 2798 2923 2955 6848 6880 2766
Size: 32 125 32 3893 32 3217
Fragments arranged by size:
3893 3217 125 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 834 2329 5128 834
Size: 1495 2799 3037
Fragments arranged by size:
3037 2799 1495
XbaI T'CTAG_A
Cuts at: 1893 4362 1893
Size: 2469 4862
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 157 690 1011 1884 3189 3676 4093 4826 157
Size: 533 321 873 1305 487 417 733 2662
Fragments arranged by size:
2662 1305 873 733 533 487 417 321
XhoI C'TCGA_G
Cuts at: 3084 3084
Size: 7331
XmaI C'CCGG_G
Cuts at: 4351 4351
Size: 7331
XmnI GAAnn'nnTTC
Cuts at: 2082 2082
Size: 7331
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI AscI BfrBI
BmgBI BmtI BsiWI BsrGI BssHII ClaI CspCI EcoNI
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PsiI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI