Restriction map for
pF1KA0369
(Circular) (Six-base) MAPSORT of: pF1KA0369.seq Check: 7053 from: 1 to: 5300
pF1KA0369 5300 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1816 1911 1816
Size: 95 5205
AccI GT'mk_AC
Cuts at: 1727 2338 1727
Size: 611 4689
Acc65I G'GTAC_C
Cuts at: 2316 2316
Size: 5300
AclI AA'CG_TT
Cuts at: 5140 5140
Size: 5300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 826 1714 3125 3557 3910 4572 611
Size: 215 888 1411 432 353 662 1339
Fragments arranged by size:
1411 1339 888 662 432 353 215
AflIII A'CryG_T
Cuts at: 812 2652 4452 4733 812
Size: 1840 1800 281 1379
Fragments arranged by size:
1840 1800 1379 281
AgeI A'CCGG_T
Cuts at: 344 344
Size: 5300
AlwNI CAG_nnn'CTG
Cuts at: 1792 2092 2146 2605 4043 1792
Size: 300 54 459 1438 3049
Fragments arranged by size:
3049 1438 459 300 54
ApaLI G'TGCA_C
Cuts at: 4138 4138
Size: 5300
ApoI r'AATT_y
Cuts at: 405 1305 2304 4653 4909 405
Size: 900 999 2349 256 796
Fragments arranged by size:
2349 999 900 796 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5300
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5300
AvaI C'yCGr_G
Cuts at: 587 789 2320 587
Size: 202 1531 3567
Fragments arranged by size:
3567 1531 202
BamHI G'GATC_C
Cuts at: 2325 2325
Size: 5300
BanI G'GyrC_C
Cuts at: 1753 2316 2730 2978 3013 1753
Size: 563 414 248 35 4040
Fragments arranged by size:
4040 563 414 248 35
BanII G_rGCy'C
Cuts at: 205 449 515 1003 1147 1976 2314 3344 205
Size: 244 66 488 144 829 338 1030 2161
Fragments arranged by size:
2161 1030 829 488 338 244 144 66
BbeI G_GCGC'C
Cuts at: 2734 2982 2734
Size: 248 5052
BbsI GAAGACnn'nnnn_
Cuts at: 590 2285 590
Size: 1695 3605
BciVI GTATCCnnnnn_n'
Cuts at: 1789 3198 4254 5300 1789
Size: 1409 1056 1046 1789
Fragments arranged by size:
1789 1409 1056 1046
BclI T'GATC_A
Cuts at: 1902 2820 1902
Size: 918 4382
BglI GCCn_nnn'nGGC
Cuts at: 370 370
Size: 5300
BglII A'GATC_T
Cuts at: 2815 4852 2815
Size: 2037 3263
BlpI GC'TnA_GC
Cuts at: 256 2412 256
Size: 2156 3144
Bme1580I G_kGCm'C
Cuts at: 2925 3018 4142 2925
Size: 93 1124 4083
Fragments arranged by size:
4083 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1953 2512 2593 2928 5072 1953
Size: 559 81 335 2144 2181
Fragments arranged by size:
2181 2144 559 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1163 3667 1163
Size: 2504 2796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 547 1241 1757 2168 154
Size: 393 694 516 411 3286
Fragments arranged by size:
3286 694 516 411 393
Bpu10I CC'TnA_GC
Cuts at: 1086 1086
Size: 5300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1789 2053 2476 3355 4069 4367 433
Size: 1356 264 423 879 714 298 1366
Fragments arranged by size:
1366 1356 879 714 423 298 264
BsaI GGTCTCn'nnnn_
Cuts at: 1514 5004 1514
Size: 3490 1810
BsaAI yAC'GTr
Cuts at: 1767 2653 1767
Size: 886 4414
BsaBI GATnn'nnATC
Cuts at: 6 2003 2814 4851 6
Size: 1997 811 2037 455
Fragments arranged by size:
2037 1997 811 455
BsaHI Gr'CG_yC
Cuts at: 1568 2731 2979 3681 5177 1568
Size: 1163 248 702 1496 1691
Fragments arranged by size:
1691 1496 1163 702 248
BsaWI w'CCGG_w
Cuts at: 344 885 2489 3010 3688 4099 4246 4520 344
Size: 541 1604 521 678 411 147 274 1124
Fragments arranged by size:
1604 1124 678 541 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1214 1244 2141 2171 1214
Size: 30 897 30 4343
Fragments arranged by size:
4343 897 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1152 1155 1480 1152
Size: 3 325 4972
Fragments arranged by size:
4972 325 3
BseYI C'CCAG_C
Cuts at: 1698 2725 3483 4148 4556 1698
Size: 1027 758 665 408 2442
Fragments arranged by size:
2442 1027 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2842 2888 4118 104
Size: 2738 46 1230 1286
Fragments arranged by size:
2738 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 140 991 1147 1400 1673 1976 2314 3092 3282
Size: 851 156 253 273 303 338 778 190
Cuts at: 3282 4142 140
Size: 860 1298
Fragments arranged by size:
1298 860 851 778 338 303 273 253
190 156
BsmBI CGTCTCn'nnnn_
Cuts at: 1559 1559
Size: 5300
Bsp1286I G_dGCh'C
Cuts at: 140 205 449 515 991 1003 1147 1400 1673
Size: 65 244 66 476 12 144 253 273
Cuts at: 1673 1976 2314 2925 3018 3092 3282 3344 4142
Size: 303 338 611 93 74 190 62 798
Cuts at: 4142 140
Size: 1298
Fragments arranged by size:
1298 798 611 476 338 303 273 253
244 190 144 93 74 66 65 62
12
BspHI T'CATG_A
Cuts at: 3732 3732
Size: 5300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 741 1619 1664 1816 1911 2255 2350 2564 2866
Size: 878 45 152 95 344 95 214 302
Cuts at: 2866 3247 741
Size: 381 2794
Fragments arranged by size:
2794 878 381 344 302 214 152 95
95 45
BsrBI CCG'CTC
Cuts at: 50 150 2247 3592 3646 5130 5295 50
Size: 100 2097 1345 54 1484 165 55
Fragments arranged by size:
2097 1484 1345 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 3212 1781
Size: 1431 3869
BsrFI r'CCGG_y
Cuts at: 344 3298 3479 344
Size: 2954 181 2165
Fragments arranged by size:
2954 2165 181
BsrGI T'GTAC_A
Cuts at: 809 1583 1739 809
Size: 774 156 4370
Fragments arranged by size:
4370 774 156
BssSI C'ACGA_G
Cuts at: 3571 4279 3571
Size: 708 4592
BstAPI GCAn_nnn'nTGC
Cuts at: 2677 2677
Size: 5300
BstBI TT'CG_AA
Cuts at: 3661 3661
Size: 5300
BstEII G'GTnAC_C
Cuts at: 669 669
Size: 5300
BstXI CCAn_nnnn'nTGG
Cuts at: 783 783
Size: 5300
BstYI r'GATC_y
Cuts at: 1 2325 2792 2815 3150 3396 3702 3714 3800
Size: 2324 467 23 335 246 306 12 86
Cuts at: 3800 4852 1
Size: 1052 449
Fragments arranged by size:
2324 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 697 1869 2172 697
Size: 1172 303 3825
Fragments arranged by size:
3825 1172 303
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3250 3431 3594 3250
Size: 181 163 4956
Fragments arranged by size:
4956 181 163
BtsI GCAGTG_nn'
Cuts at: 219 599 608 2543 219
Size: 380 9 1935 2976
Fragments arranged by size:
2976 1935 380 9
ClaI AT'CG_AT
Cuts at: 306 306
Size: 5300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1748 1783 2231 2266 1748
Size: 35 448 35 4782
Fragments arranged by size:
4782 448 35 35
DraI TTT'AAA
Cuts at: 2299 2299
Size: 5300
DrdI GACnn_nn'nnGTC
Cuts at: 3006 4350 3006
Size: 1344 3956
EaeI y'GGCC_r
Cuts at: 605 1715 2885 3059 3450 3477 4822 605
Size: 1110 1170 174 391 27 1345 1083
Fragments arranged by size:
1345 1170 1110 1083 391 174 27
EagI C'GGCC_G
Cuts at: 2885 2885
Size: 5300
EarI CTCTTCn'nnn_
Cuts at: 1425 3323 3533 1425
Size: 1898 210 3192
Fragments arranged by size:
3192 1898 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3508 4250 4396 5110 3508
Size: 742 146 714 3698
Fragments arranged by size:
3698 742 714 146
EcoICRI GAG'CTC
Cuts at: 1145 1974 2312 1145
Size: 829 338 4133
Fragments arranged by size:
4133 829 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 547 611 826 1241 1714 1757 2168 3125
Size: 393 64 215 415 473 43 411 957
Cuts at: 3125 3557 3910 4572 154
Size: 432 353 662 882
Fragments arranged by size:
957 882 662 473 432 415 411 393
353 215 64 43
EcoO109I rG'GnC_Cy
Cuts at: 363 753 2122 2439 363
Size: 390 1369 317 3224
Fragments arranged by size:
3224 1369 390 317
EcoRI G'AATT_C
Cuts at: 2304 2304
Size: 5300
EcoRV GAT'ATC
Cuts at: 765 1641 765
Size: 876 4424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3319 3319
Size: 5300
FspI TGC'GCA
Cuts at: 3081 3081
Size: 5300
HaeII r_GCGC'y
Cuts at: 218 1601 2734 2982 4212 4983 218
Size: 1383 1133 248 1230 771 535
Fragments arranged by size:
1383 1230 1133 771 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 372 404 1265 1297 1787 1819 2141 2173 2231
Size: 32 861 32 490 32 322 32 58
Cuts at: 2231 2263 3142 3172 3174 3204 372
Size: 32 879 30 2 30 2468
Fragments arranged by size:
2468 879 861 490 322 58 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2339 2339
Size: 5300
KasI G'GCGC_C
Cuts at: 2730 2978 2730
Size: 248 5052
KpnI G_GTAC'C
Cuts at: 2320 2320
Size: 5300
MluI A'CGCG_T
Cuts at: 4733 4733
Size: 5300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 374 797 1214 1263 2297 4059 4243 374
Size: 423 417 49 1034 1762 184 1431
Fragments arranged by size:
1762 1431 1034 423 417 184 49
MscI TGG'CCA
Cuts at: 607 1717 3061 607
Size: 1110 1344 2846
Fragments arranged by size:
2846 1344 1110
MslI CAynn'nnrTG
Cuts at: 1676 1981 3289 3416 1676
Size: 305 1308 127 3560
Fragments arranged by size:
3560 1308 305 127
MspA1I CmG'CkG
Cuts at: 2082 2146 2411 2725 3085 3869 4114 2082
Size: 64 265 314 360 784 245 3268
Fragments arranged by size:
3268 784 360 314 265 245 64
NaeI GCC'GGC
Cuts at: 3481 3481
Size: 5300
NarI GG'CG_CC
Cuts at: 2731 2979 2731
Size: 248 5052
NgoMIV G'CCGG_C
Cuts at: 3479 3479
Size: 5300
NspI r_CATG'y
Cuts at: 881 2353 2853 4456 881
Size: 1472 500 1603 1725
Fragments arranged by size:
1725 1603 1472 500
PasI CC'CwG_GG
Cuts at: 414 2042 414
Size: 1628 3672
PciI A'CATG_T
Cuts at: 4452 4452
Size: 5300
PflMI CCAn_nnn'nTGG
Cuts at: 477 1870 477
Size: 1393 3907
PmeI GTTT'AAAC
Cuts at: 2299 2299
Size: 5300
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 5268
PpuMI rG'GwC_Cy
Cuts at: 753 753
Size: 5300
PstI C_TGCA'G
Cuts at: 226 251 1195 2347 3032 226
Size: 25 944 1152 685 2494
Fragments arranged by size:
2494 1152 944 685 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5300
PvuII CAG'CTG
Cuts at: 2082 2146 2725 3085 2082
Size: 64 579 360 4297
Fragments arranged by size:
4297 579 360 64
RsrII CG'GwC_CG
Cuts at: 3495 3495
Size: 5300
SacI G_AGCT'C
Cuts at: 1147 1976 2314 1147
Size: 829 338 4133
Fragments arranged by size:
4133 829 338
SalI G'TCGA_C
Cuts at: 2337 2337
Size: 5300
SapI GCTCTTCn'nnn_
Cuts at: 3323 3533 3323
Size: 210 5090
SbfI CC_TGCA'GG
Cuts at: 2347 2347
Size: 5300
ScaI AGT'ACT
Cuts at: 2030 4817 2030
Size: 2787 2513
SfcI C'TryA_G
Cuts at: 33 222 247 1191 2343 3028 3996 4187 33
Size: 189 25 944 1152 685 968 191 1146
Fragments arranged by size:
1152 1146 968 944 685 191 189 25
SfoI GGC'GCC
Cuts at: 2732 2980 2732
Size: 248 5052
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5300
SmaI CCC'GGG
Cuts at: 2322 2322
Size: 5300
SmlI C'TyrA_G
Cuts at: 448 1804 2032 2455 3370 4084 4346 448
Size: 1356 228 423 915 714 262 1402
Fragments arranged by size:
1402 1356 915 714 423 262 228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5300
SphI G_CATG'C
Cuts at: 2353 2853 2353
Size: 500 4800
StyI C'CwwG_G
Cuts at: 2434 2788 2434
Size: 354 4946
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1125 1690 2883 3016 3475 3684 1125
Size: 565 1193 133 459 209 2741
Fragments arranged by size:
2741 1193 565 459 209 133
TatI w'GTAC_w
Cuts at: 530 809 1583 1739 2028 4815 530
Size: 279 774 156 289 2787 1015
Fragments arranged by size:
2787 1015 774 289 279 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1214 1246 4817 4849 1214
Size: 32 3571 32 1665
Fragments arranged by size:
3571 1665 32 32
Tth111I GACn'n_nGTC
Cuts at: 3097 3097
Size: 5300
XbaI T'CTAG_A
Cuts at: 2331 2331
Size: 5300
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1989 2228 2795 1989
Size: 239 567 4494
Fragments arranged by size:
4494 567 239
XmaI C'CCGG_G
Cuts at: 2320 2320
Size: 5300
XmnI GAAnn'nnTTC
Cuts at: 624 2110 624
Size: 1486 3814
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BspEI BssHII BstZ17I Bsu36I DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI