Restriction map for
pF1KA0366
(Circular) (Six-base) MAPSORT of: pF1KA0366.seq Check: 4353 from: 1 to: 6728
pF1KA0366 6728 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 3696 3696
Size: 6728
AccI GT'mk_AC
Cuts at: 707 3766 707
Size: 3059 3669
Acc65I G'GTAC_C
Cuts at: 2288 3744 2288
Size: 1456 5272
AclI AA'CG_TT
Cuts at: 6568 6568
Size: 6728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 1926 2893 3413 4553 4985 5732 6000 146
Size: 1780 967 520 1140 432 747 268 874
Fragments arranged by size:
1780 1140 967 874 747 520 432 268
AflII C'TTAA_G
Cuts at: 2293 2293
Size: 6728
AflIII A'CryG_T
Cuts at: 323 743 1804 2325 4080 5184 6161 323
Size: 420 1061 521 1755 1104 977 890
Fragments arranged by size:
1755 1104 1061 977 890 521 420
AgeI A'CCGG_T
Cuts at: 5116 5116
Size: 6728
AleI CACnn'nnGTG
Cuts at: 1836 1953 1836
Size: 117 6611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1051 1229 2161 3231 1051
Size: 178 932 1070 4548
Fragments arranged by size:
4548 1070 932 178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1802 1834 3024 3056 1802
Size: 32 1190 32 5474
Fragments arranged by size:
5474 1190 32 32
AlwNI CAG_nnn'CTG
Cuts at: 599 828 2762 3134 4033 5600 599
Size: 229 1934 372 899 1567 1727
Fragments arranged by size:
1934 1727 1567 899 372 229
ApaLI G'TGCA_C
Cuts at: 1584 2941 5498 1584
Size: 1357 2557 2814
Fragments arranged by size:
2814 2557 1357
ApoI r'AATT_y
Cuts at: 977 1783 3732 6081 6337 977
Size: 806 1949 2349 256 1368
Fragments arranged by size:
2349 1949 1368 806 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6728
AvaI C'yCGr_G
Cuts at: 2971 3748 2971
Size: 777 5951
BamHI G'GATC_C
Cuts at: 3753 3753
Size: 6728
BanI G'GyrC_C
Cuts at: 453 2288 2926 3744 4158 4406 4441 453
Size: 1835 638 818 414 248 35 2740
Fragments arranged by size:
2740 1835 818 638 414 248 35
BanII G_rGCy'C
Cuts at: 3742 4772 3742
Size: 1030 5698
BbeI G_GCGC'C
Cuts at: 4162 4410 4162
Size: 248 6480
BbsI GAAGACnn'nnnn_
Cuts at: 320 2348 2482 2597 320
Size: 2028 134 115 4451
Fragments arranged by size:
4451 2028 134 115
BciVI GTATCCnnnnn_n'
Cuts at: 4626 5387 6728 4626
Size: 761 1341 4626
Fragments arranged by size:
4626 1341 761
BclI T'GATC_A
Cuts at: 1491 4248 1491
Size: 2757 3971
BfrBI ATG'CAT
Cuts at: 3448 3448
Size: 6728
BglII A'GATC_T
Cuts at: 1129 2442 3349 4243 6280 1129
Size: 1313 907 894 2037 1577
Fragments arranged by size:
2037 1577 1313 907 894
BlpI GC'TnA_GC
Cuts at: 3509 3840 3509
Size: 331 6397
Bme1580I G_kGCm'C
Cuts at: 1588 2945 3002 4353 4446 5502 1588
Size: 1357 57 1351 93 1056 2814
Fragments arranged by size:
2814 1357 1351 1056 93 57
BmgBI CAC'GTC
Cuts at: 324 936 324
Size: 612 6116
BmrI ACTGGGnnnn_n'
Cuts at: 3940 4021 4356 6500 3940
Size: 81 335 2144 4168
Fragments arranged by size:
4168 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2687 3524 5095 2687
Size: 837 1571 4320
Fragments arranged by size:
4320 1571 837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1202 2090 3051 230
Size: 972 888 961 3907
Fragments arranged by size:
3907 972 961 888
Bpu10I CC'TnA_GC
Cuts at: 338 338
Size: 6728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 3904 4783 5275 5573 2525
Size: 1379 879 492 298 3680
Fragments arranged by size:
3680 1379 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2675 3385 6432 2675
Size: 710 3047 2971
Fragments arranged by size:
3047 2971 710
BsaAI yAC'GTr
Cuts at: 506 4081 506
Size: 3575 3153
BsaBI GATnn'nnATC
Cuts at: 6 380 4242 6279 6
Size: 374 3862 2037 455
Fragments arranged by size:
3862 2037 455 374
BsaHI Gr'CG_yC
Cuts at: 846 1114 3693 4159 4407 5109 6605 846
Size: 268 2579 466 248 702 1496 969
Fragments arranged by size:
2579 1496 969 702 466 268 248
BsaWI w'CCGG_w
Cuts at: 515 3917 4438 5116 5390 5537 5948 515
Size: 3402 521 678 274 147 411 1295
Fragments arranged by size:
3402 1295 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1087 1117 2144 2174 2256 2286 3024 3054 1087
Size: 30 1027 30 82 30 738 30 4761
Fragments arranged by size:
4761 1027 738 82 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2165 2240 2345 3516 2165
Size: 75 105 1171 5377
Fragments arranged by size:
5377 1171 105 75
BseYI C'CCAG_C
Cuts at: 891 1038 1109 3091 4153 4911 5488 5984 891
Size: 147 71 1982 1062 758 577 496 1635
Fragments arranged by size:
1982 1635 1062 758 577 496 147 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2841 2993 2841
Size: 152 6576
BsiEI CG_ry'CG
Cuts at: 104 4270 4316 5524 104
Size: 4166 46 1208 1308
Fragments arranged by size:
4166 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 935 1588 1950 2945 3510 3742 4520 4710 5502
Size: 653 362 995 565 232 778 190 792
Cuts at: 5502 935
Size: 2161
Fragments arranged by size:
2161 995 792 778 653 565 362 232
190
BsmI GAATG_Cn'
Cuts at: 1208 1402 1743 1924 1208
Size: 194 341 181 6012
Fragments arranged by size:
6012 341 194 181
BsmBI CGTCTCn'nnnn_
Cuts at: 837 837
Size: 6728
Bsp1286I G_dGCh'C
Cuts at: 935 1588 1950 2945 3002 3510 3742 4353 4446
Size: 653 362 995 57 508 232 611 93
Cuts at: 4446 4520 4710 4772 5502 935
Size: 74 190 62 730 2161
Fragments arranged by size:
2161 995 730 653 611 508 362 232
190 93 74 62 57
BspEI T'CCGG_A
Cuts at: 515 5948 515
Size: 5433 1295
BspHI T'CATG_A
Cuts at: 1263 1494 3312 5904 1263
Size: 231 1818 2592 2087
Fragments arranged by size:
2592 2087 1818 231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1203 3058 3066 3778 3992 4294 4675 1203
Size: 1855 8 712 214 302 381 3256
Fragments arranged by size:
3256 1855 712 381 302 214 8
BsrBI CCG'CTC
Cuts at: 50 142 2558 2936 5020 5074 6558 6723 50
Size: 92 2416 378 2084 54 1484 165 55
Fragments arranged by size:
2416 2084 1484 378 165 92 55 54
BsrDI GCAATG_nn'
Cuts at: 1555 1722 4640 1555
Size: 167 2918 3643
Fragments arranged by size:
3643 2918 167
BsrFI r'CCGG_y
Cuts at: 1225 2981 4726 4907 5116 1225
Size: 1756 1745 181 209 2837
Fragments arranged by size:
2837 1756 1745 209 181
BsrGI T'GTAC_A
Cuts at: 2818 2818
Size: 6728
BssSI C'ACGA_G
Cuts at: 4999 5357 4999
Size: 358 6370
BstAPI GCAn_nnn'nTGC
Cuts at: 2954 3454 4105 2954
Size: 500 651 5577
Fragments arranged by size:
5577 651 500
BstBI TT'CG_AA
Cuts at: 5089 5089
Size: 6728
BstEII G'GTnAC_C
Cuts at: 2962 3540 2962
Size: 578 6150
BstXI CCAn_nnnn'nTGG
Cuts at: 1092 3106 1092
Size: 2014 4714
BstYI r'GATC_y
Cuts at: 1 1129 2127 2442 3349 3753 4220 4243 4578
Size: 1128 998 315 907 404 467 23 335
Cuts at: 4578 4824 5836 5922 5934 6280 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1128 1012 998 907 467 449 404 346
335 315 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 921 107
Size: 814 5914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2737 4678 4859 5022 2737
Size: 1941 181 163 4443
Fragments arranged by size:
4443 1941 181 163
BtsI GCAGTG_nn'
Cuts at: 1437 1960 2238 2853 3971 1437
Size: 523 278 615 1118 4194
Fragments arranged by size:
4194 1118 615 523 278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1332 1367 2670 2705 2850 2885 1332
Size: 35 1303 35 145 35 5175
Fragments arranged by size:
5175 1303 145 35 35 35
DraI TTT'AAA
Cuts at: 2402 3727 2402
Size: 1325 5403
DrdI GACnn_nn'nnGTC
Cuts at: 910 4434 5292 910
Size: 3524 858 2346
Fragments arranged by size:
3524 2346 858
EaeI y'GGCC_r
Cuts at: 4313 4487 4878 4905 6250 4313
Size: 174 391 27 1345 4791
Fragments arranged by size:
4791 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4313 4313
Size: 6728
EarI CTCTTCn'nnn_
Cuts at: 632 2656 3432 4751 4961 632
Size: 2024 776 1319 210 2399
Fragments arranged by size:
2399 2024 1319 776 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4936 5246 5392 6538 4936
Size: 310 146 1146 5126
Fragments arranged by size:
5126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3740 3740
Size: 6728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 230 1202 1926 2090 2893 3051 3413 4553
Size: 84 972 724 164 803 158 362 1140
Cuts at: 4553 4985 5732 6000 146
Size: 432 747 268 874
Fragments arranged by size:
1140 972 874 803 747 724 432 362
268 164 158 84
EcoO109I rG'GnC_Cy
Cuts at: 1669 3025 3078 3867 1669
Size: 1356 53 789 4530
Fragments arranged by size:
4530 1356 789 53
EcoRI G'AATT_C
Cuts at: 3732 3732
Size: 6728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4747 4747
Size: 6728
FspI TGC'GCA
Cuts at: 1027 4509 1027
Size: 3482 3246
FspAI rTGC'GCAy
Cuts at: 1027 1027
Size: 6728
HaeII r_GCGC'y
Cuts at: 4162 4410 5432 6411 4162
Size: 248 1022 979 4479
Fragments arranged by size:
4479 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 260 292 1121 1153 2523 2542 2555 2574 3310
Size: 32 829 32 1370 19 13 19 736
Cuts at: 3310 3342 4570 4600 4602 4632 260
Size: 32 1228 30 2 30 2356
Fragments arranged by size:
2356 1370 1228 829 736 32 32 32
30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 3139 3767 3139
Size: 628 6100
HindIII A'AGCT_T
Cuts at: 1089 2281 1089
Size: 1192 5536
KasI G'GCGC_C
Cuts at: 4158 4406 4158
Size: 248 6480
KpnI G_GTAC'C
Cuts at: 2292 3748 2292
Size: 1456 5272
MfeI C'AATT_G
Cuts at: 1764 1764
Size: 6728
MluI A'CGCG_T
Cuts at: 6161 6161
Size: 6728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 752 3721 5399 5583 356
Size: 396 2969 1678 184 1501
Fragments arranged by size:
2969 1678 1501 396 184
MscI TGG'CCA
Cuts at: 4489 4489
Size: 6728
MslI CAynn'nnrTG
Cuts at: 742 1010 1033 1160 1228 1268 1463 1836 1953
Size: 268 23 127 68 40 195 373 117
Cuts at: 1953 2143 2498 3104 3317 4717 4844 742
Size: 190 355 606 213 1400 127 2626
Fragments arranged by size:
2626 1400 606 373 355 268 213 195
190 127 127 117 68 40 23
MspA1I CmG'CkG
Cuts at: 166 721 828 1620 3839 4153 4513 5526 5771
Size: 555 107 792 2219 314 360 1013 245
Cuts at: 5771 166
Size: 1123
Fragments arranged by size:
2219 1123 1013 792 555 360 314 245
107
NaeI GCC'GGC
Cuts at: 2983 4909 2983
Size: 1926 4802
NarI GG'CG_CC
Cuts at: 4159 4407 4159
Size: 248 6480
NcoI C'CATG_G
Cuts at: 107 921 107
Size: 814 5914
NdeI CA'TA_TG
Cuts at: 2013 2140 3450 2013
Size: 127 1310 5291
Fragments arranged by size:
5291 1310 127
NgoMIV G'CCGG_C
Cuts at: 2981 4907 2981
Size: 1926 4802
NsiI A_TGCA'T
Cuts at: 3450 3450
Size: 6728
NspI r_CATG'y
Cuts at: 747 1233 1731 1808 2329 3781 4281 5188 747
Size: 486 498 77 521 1452 500 907 2287
Fragments arranged by size:
2287 1452 907 521 500 498 486 77
PasI CC'CwG_GG
Cuts at: 2314 2314
Size: 6728
PciI A'CATG_T
Cuts at: 743 1804 2325 5184 743
Size: 1061 521 2859 2287
Fragments arranged by size:
2859 2287 1061 521
PflMI CCAn_nnn'nTGG
Cuts at: 1317 1681 1317
Size: 364 6364
PfoI T'CCnGG_A
Cuts at: 573 1521 2627 573
Size: 948 1106 4674
Fragments arranged by size:
4674 1106 948
PmeI GTTT'AAAC
Cuts at: 3727 3727
Size: 6728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 331 363 3024 3056 5867 5899 331
Size: 32 2661 32 2811 32 1160
Fragments arranged by size:
2811 2661 1160 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1669 3025 3078 1669
Size: 1356 53 5319
Fragments arranged by size:
5319 1356 53
PshAI GACnn'nnGTC
Cuts at: 247 247
Size: 6728
PsiI TTA'TAA
Cuts at: 2465 3233 2465
Size: 768 5960
PstI C_TGCA'G
Cuts at: 3088 3259 3775 4460 3088
Size: 171 516 685 5356
Fragments arranged by size:
5356 685 516 171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6728
PvuII CAG'CTG
Cuts at: 166 721 828 1620 4153 4513 166
Size: 555 107 792 2533 360 2381
Fragments arranged by size:
2533 2381 792 555 360 107
RsrII CG'GwC_CG
Cuts at: 4923 4923
Size: 6728
SacI G_AGCT'C
Cuts at: 3742 3742
Size: 6728
SalI G'TCGA_C
Cuts at: 3765 3765
Size: 6728
SapI GCTCTTCn'nnn_
Cuts at: 2656 4751 4961 2656
Size: 2095 210 4423
Fragments arranged by size:
4423 2095 210
SbfI CC_TGCA'GG
Cuts at: 3775 3775
Size: 6728
ScaI AGT'ACT
Cuts at: 6245 6245
Size: 6728
SexAI A'CCwGG_T
Cuts at: 3225 3225
Size: 6728
SfcI C'TryA_G
Cuts at: 33 722 762 2384 3084 3255 3771 4456 5449
Size: 689 40 1622 700 171 516 685 993
Cuts at: 5449 5640 33
Size: 191 1121
Fragments arranged by size:
1622 1121 993 700 689 685 516 191
171 40
SfoI GGC'GCC
Cuts at: 4160 4408 4160
Size: 248 6480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6728
SmaI CCC'GGG
Cuts at: 3750 3750
Size: 6728
SmlI C'TyrA_G
Cuts at: 2293 2540 3883 4798 5290 5552 2293
Size: 247 1343 915 492 262 3469
Fragments arranged by size:
3469 1343 915 492 262 247
SnaBI TAC'GTA
Cuts at: 506 506
Size: 6728
SpeI A'CTAG_T
Cuts at: 67 408 67
Size: 341 6387
SphI G_CATG'C
Cuts at: 3781 4281 3781
Size: 500 6228
SspI AAT'ATT
Cuts at: 2808 2808
Size: 6728
StuI AGG'CCT
Cuts at: 785 785
Size: 6728
StyI C'CwwG_G
Cuts at: 107 656 921 1388 2060 3862 4216 107
Size: 549 265 467 672 1802 354 2619
Fragments arranged by size:
2619 1802 672 549 467 354 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2950 3114 3576 4311 4444 4903 5112 2950
Size: 164 462 735 133 459 209 4566
Fragments arranged by size:
4566 735 462 459 209 164 133
TatI w'GTAC_w
Cuts at: 2576 2705 2818 6243 2576
Size: 129 113 3425 3061
Fragments arranged by size:
3425 3061 129 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1087 1119 1677 1709 2144 2176 3053 3085 6245
Size: 32 558 32 435 32 877 32 3160
Cuts at: 6245 6277 1087
Size: 32 1538
Fragments arranged by size:
3160 1538 877 558 435 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4525 4525
Size: 6728
XbaI T'CTAG_A
Cuts at: 261 3294 3759 261
Size: 3033 465 3230
Fragments arranged by size:
3230 3033 465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4223 4223
Size: 6728
XmaI C'CCGG_G
Cuts at: 3748 3748
Size: 6728
XmnI GAAnn'nnTTC
Cuts at: 2489 2489
Size: 6728
ZraI GAC'GTC
Cuts at: 3694 3694
Size: 6728
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI ApaI AscI AvrII BaeI BbvCI
BcgI BglI BmtI BsiWI BssHII BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI HpaI NheI NotI NruI
PacI PmlI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SwaI XhoI