Restriction map for
pF1KA0363
(Circular) (Six-base) MAPSORT of: pF1KA0363.seq Check: 1700 from: 1 to: 7520
pF1KA0363 7520 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 4558 4558
Size: 7520
Acc65I G'GTAC_C
Cuts at: 3526 4536 3526
Size: 1010 6510
AclI AA'CG_TT
Cuts at: 7360 7360
Size: 7520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 1739 1859 1979 2099 2219 2339 2459 2579
Size: 1164 120 120 120 120 120 120 120
Cuts at: 2579 3100 3278 5345 5777 6130 6792 575
Size: 521 178 2067 432 353 662 1303
Fragments arranged by size:
2067 1303 1164 662 521 432 353 178
120 120 120 120 120 120 120
AfeI AGC'GCT
Cuts at: 242 242
Size: 7520
AflIII A'CryG_T
Cuts at: 4872 6672 6953 4872
Size: 1800 281 5439
Fragments arranged by size:
5439 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 897 897
Size: 7520
AleI CACnn'nnGTG
Cuts at: 1702 1822 1942 2062 2182 2302 2422 2542 3008
Size: 120 120 120 120 120 120 120 466
Cuts at: 3008 3487 4210 1702
Size: 479 723 5012
Fragments arranged by size:
5012 723 479 466 120 120 120 120
120 120 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3457 3457
Size: 7520
AlwNI CAG_nnn'CTG
Cuts at: 141 337 433 921 2707 2764 2854 3326 3699
Size: 196 96 488 1786 57 90 472 373
Cuts at: 3699 4825 6263 141
Size: 1126 1438 1398
Fragments arranged by size:
1786 1438 1398 1126 488 472 373 196
96 90 57
ApaI G_GGCC'C
Cuts at: 407 3508 3830 3915 407
Size: 3101 322 85 4012
Fragments arranged by size:
4012 3101 322 85
ApaLI G'TGCA_C
Cuts at: 4255 6358 4255
Size: 2103 5417
ApoI r'AATT_y
Cuts at: 4524 6873 7129 4524
Size: 2349 256 4915
Fragments arranged by size:
4915 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7520
AvaI C'yCGr_G
Cuts at: 286 477 3865 3908 3921 4331 4540 286
Size: 191 3388 43 13 410 209 3266
Fragments arranged by size:
3388 3266 410 209 191 43 13
BamHI G'GATC_C
Cuts at: 849 4545 849
Size: 3696 3824
BanI G'GyrC_C
Cuts at: 3433 3526 4284 4536 4950 5198 5233 3433
Size: 93 758 252 414 248 35 5720
Fragments arranged by size:
5720 758 414 252 248 93 35
BanII G_rGCy'C
Cuts at: 179 407 484 647 1055 3193 3239 3508 3563
Size: 228 77 163 408 2138 46 269 55
Cuts at: 3563 3620 3830 3838 3857 3915 3922 4195 4298
Size: 57 210 8 19 58 7 273 103
Cuts at: 4298 4464 4534 5564 179
Size: 166 70 1030 2135
Fragments arranged by size:
2138 2135 1030 408 273 269 228 210
166 163 103 77 70 58 57 55
46 19 8 7
BbeI G_GCGC'C
Cuts at: 4954 5202 4954
Size: 248 7272
BbsI GAAGACnn'nnnn_
Cuts at: 122 1099 3284 3602 3614 3905 4176 122
Size: 977 2185 318 12 291 271 3466
Fragments arranged by size:
3466 2185 977 318 291 271 12
BbvCI CC'TCA_GC
Cuts at: 535 1403 3249 3745 4299 535
Size: 868 1846 496 554 3756
Fragments arranged by size:
3756 1846 868 554 496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 943 964 977 998 90
Size: 34 819 21 13 21 6612
Fragments arranged by size:
6612 819 34 21 21 13
BciVI GTATCCnnnnn_n'
Cuts at: 5418 6474 7520 5418
Size: 1056 1046 5418
Fragments arranged by size:
5418 1056 1046
BclI T'GATC_A
Cuts at: 5040 5040
Size: 7520
BglI GCCn_nnn'nGGC
Cuts at: 701 1165 2719 3218 3265 3842 4084 4459 701
Size: 464 1554 499 47 577 242 375 3762
Fragments arranged by size:
3762 1554 577 499 464 375 242 47
BglII A'GATC_T
Cuts at: 1430 1662 1902 2022 2142 2262 2382 2502 3791
Size: 232 240 120 120 120 120 120 1289
Cuts at: 3791 5035 7072 1430
Size: 1244 2037 1878
Fragments arranged by size:
2037 1878 1289 1244 240 232 120 120
120 120 120
BlpI GC'TnA_GC
Cuts at: 434 2723 3020 4632 434
Size: 2289 297 1612 3322
Fragments arranged by size:
3322 2289 1612 297
Bme1580I G_kGCm'C
Cuts at: 266 407 2694 3508 3830 3915 4259 4289 5145
Size: 141 2287 814 322 85 344 30 856
Cuts at: 5145 5238 6362 266
Size: 93 1124 1424
Fragments arranged by size:
2287 1424 1124 856 814 344 322 141
93 85 30
BmgBI CAC'GTC
Cuts at: 203 899 3350 203
Size: 696 2451 4373
Fragments arranged by size:
4373 2451 696
BmrI ACTGGGnnnn_n'
Cuts at: 3394 3416 3716 4732 4813 5148 7292 3394
Size: 22 300 1016 81 335 2144 3622
Fragments arranged by size:
3622 2144 1016 335 300 81 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2946 3739 3918 5887 2946
Size: 793 179 1969 4579
Fragments arranged by size:
4579 1969 793 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1060 1294 3070 3577 3913 4363 193
Size: 867 234 1776 507 336 450 3350
Fragments arranged by size:
3350 1776 867 507 450 336 234
Bpu10I CC'TnA_GC
Cuts at: 244 535 1136 1403 2921 3187 3249 3745 4299
Size: 291 601 267 1518 266 62 496 554
Cuts at: 4299 244
Size: 3465
Fragments arranged by size:
3465 1518 601 554 496 291 267 266
62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3833 4696 5575 6289 6587 3833
Size: 863 879 714 298 4766
Fragments arranged by size:
4766 879 863 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3037 4030 7224 3037
Size: 993 3194 3333
Fragments arranged by size:
3333 3194 993
BsaAI yAC'GTr
Cuts at: 4873 4873
Size: 7520
BsaBI GATnn'nnATC
Cuts at: 6 4135 5034 7071 6
Size: 4129 899 2037 455
Fragments arranged by size:
4129 2037 899 455
BsaHI Gr'CG_yC
Cuts at: 416 4951 5199 5901 7397 416
Size: 4535 248 702 1496 539
Fragments arranged by size:
4535 1496 702 539 248
BsaWI w'CCGG_w
Cuts at: 4709 5230 5908 6319 6466 6740 4709
Size: 521 678 411 147 274 5489
Fragments arranged by size:
5489 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 138 168 667 697 994 1024 138
Size: 30 499 30 297 30 6634
Fragments arranged by size:
6634 499 297 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 103 146 211 675 850 901 981 1114 1117
Size: 43 65 464 175 51 80 133 3
Cuts at: 1117 1163 1502 2782 3628 3733 3806 3977 4048
Size: 46 339 1280 846 105 73 171 71
Cuts at: 4048 4378 4381 4384 103
Size: 330 3 3 3239
Fragments arranged by size:
3239 1280 846 464 339 330 175 171
133 105 80 73 71 65 51 46
43 3 3 3
BseYI C'CCAG_C
Cuts at: 509 578 627 874 1345 1709 1829 1949 2069
Size: 69 49 247 471 364 120 120 120
Cuts at: 2069 2189 2309 2429 2549 2854 2938 3192 3365
Size: 120 120 120 120 305 84 254 173
Cuts at: 3365 3478 3535 3564 3837 4017 4098 4194 4247
Size: 113 57 29 273 180 81 96 53
Cuts at: 4247 4945 5703 6368 6776 509
Size: 698 758 665 408 1253
Fragments arranged by size:
1253 758 698 665 471 408 364 305
273 254 247 180 173 120 120 120
120 120 120 120 113 96 84 81
69 57 53 49 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 3338 4018 607
Size: 2731 680 4109
Fragments arranged by size:
4109 2731 680
BsiEI CG_ry'CG
Cuts at: 104 497 5062 5108 6338 104
Size: 393 4565 46 1230 1286
Fragments arranged by size:
4565 1286 1230 393 46
BsiHKAI G_wGCw'C
Cuts at: 4259 4534 5312 5502 6362 4259
Size: 275 778 190 860 5417
Fragments arranged by size:
5417 860 778 275 190
BsmI GAATG_Cn'
Cuts at: 2781 3930 2781
Size: 1149 6371
BsmBI CGTCTCn'nnnn_
Cuts at: 193 193
Size: 7520
Bsp1286I G_dGCh'C
Cuts at: 179 266 407 484 647 1055 2694 3193 3239
Size: 87 141 77 163 408 1639 499 46
Cuts at: 3239 3508 3563 3620 3830 3838 3857 3915 3922
Size: 269 55 57 210 8 19 58 7
Cuts at: 3922 4195 4259 4289 4298 4464 4534 5145 5238
Size: 273 64 30 9 166 70 611 93
Cuts at: 5238 5312 5502 5564 6362 179
Size: 74 190 62 798 1337
Fragments arranged by size:
1639 1337 798 611 499 408 273 269
210 190 166 163 141 93 87 77
74 70 64 62 58 57 55 46
30 19 9 8 7
BspHI T'CATG_A
Cuts at: 5952 5952
Size: 7520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1284 4570 4784 5086 5467 1284
Size: 3286 214 302 381 3337
Fragments arranged by size:
3337 3286 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1109 5812 5866 7350 7515 50
Size: 1059 4703 54 1484 165 55
Fragments arranged by size:
4703 1484 1059 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1403 4094 5432 1403
Size: 2691 1338 3491
Fragments arranged by size:
3491 2691 1338
BsrFI r'CCGG_y
Cuts at: 2925 5518 5699 2925
Size: 2593 181 4746
Fragments arranged by size:
4746 2593 181
BsrGI T'GTAC_A
Cuts at: 1040 1040
Size: 7520
BssSI C'ACGA_G
Cuts at: 5791 6499 5791
Size: 708 6812
BstAPI GCAn_nnn'nTGC
Cuts at: 2689 3795 4897 2689
Size: 1106 1102 5312
Fragments arranged by size:
5312 1106 1102
BstBI TT'CG_AA
Cuts at: 5881 5881
Size: 7520
BstXI CCAn_nnnn'nTGG
Cuts at: 259 3780 259
Size: 3521 3999
BstYI r'GATC_y
Cuts at: 1 849 1430 1662 1902 2022 2142 2262 2382
Size: 848 581 232 240 120 120 120 120
Cuts at: 2382 2502 3791 4545 5012 5035 5370 5616 5922
Size: 120 1289 754 467 23 335 246 306
Cuts at: 5922 5934 6020 7072 1
Size: 12 86 1052 449
Fragments arranged by size:
1289 1052 848 754 581 467 449 335
306 246 240 232 120 120 120 120
120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 119 573 2631 2745 2765 3164 119
Size: 454 2058 114 20 399 4475
Fragments arranged by size:
4475 2058 454 399 114 20
BtgI C'CryG_G
Cuts at: 146 408 602 1160 1391 2717 3488 3764 3942
Size: 262 194 558 231 1326 771 276 178
Cuts at: 3942 4511 146
Size: 569 3155
Fragments arranged by size:
3155 1326 771 569 558 276 262 231
194 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4026 4152 5470 5651 5814 4026
Size: 126 1318 181 163 5732
Fragments arranged by size:
5732 1318 181 163 126
BtsI GCAGTG_nn'
Cuts at: 803 1265 1451 3402 4006 4035 4071 4763 803
Size: 462 186 1951 604 29 36 692 3560
Fragments arranged by size:
3560 1951 692 604 462 186 36 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1667 1702 1787 1822 1907 1942 2027 2062 2147
Size: 35 85 35 85 35 85 35 85
Cuts at: 2147 2182 2267 2302 2387 2422 2507 2542 1667
Size: 35 85 35 85 35 85 35 6645
Fragments arranged by size:
6645 85 85 85 85 85 85 85
35 35 35 35 35 35 35 35
DraI TTT'AAA
Cuts at: 4519 4519
Size: 7520
DraIII CAC_nnn'GTG
Cuts at: 1499 1499
Size: 7520
DrdI GACnn_nn'nnGTC
Cuts at: 5226 6570 5226
Size: 1344 6176
EaeI y'GGCC_r
Cuts at: 164 494 728 2654 2711 3257 5105 5279 5670
Size: 330 234 1926 57 546 1848 174 391
Cuts at: 5670 5697 7042 164
Size: 27 1345 642
Fragments arranged by size:
1926 1848 1345 642 546 391 330 234
174 57 27
EagI C'GGCC_G
Cuts at: 494 5105 494
Size: 4611 2909
EarI CTCTTCn'nnn_
Cuts at: 522 827 1232 1715 1835 1955 2075 2195 2315
Size: 305 405 483 120 120 120 120 120
Cuts at: 2315 2435 2555 4349 4355 4370 5543 5753 522
Size: 120 120 1794 6 15 1173 210 2289
Fragments arranged by size:
2289 1794 1173 483 405 305 210 120
120 120 120 120 120 120 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 862 5728 6470 6616 7330 862
Size: 4866 742 146 714 1052
Fragments arranged by size:
4866 1052 742 714 146
EcoICRI GAG'CTC
Cuts at: 4532 4532
Size: 7520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 575 1060 1294 1739 1859 1979 2099 2219
Size: 382 485 234 445 120 120 120 120
Cuts at: 2219 2339 2459 2579 3070 3100 3278 3577 3913
Size: 120 120 120 491 30 178 299 336
Cuts at: 3913 4363 5345 5777 6130 6792 193
Size: 450 982 432 353 662 921
Fragments arranged by size:
982 921 662 491 485 450 445 432
382 353 336 299 234 178 120 120
120 120 120 120 120 30
EcoNI CCTnn'n_nnAGG
Cuts at: 118 3466 3580 118
Size: 3348 114 4058
Fragments arranged by size:
4058 3348 114
EcoO109I rG'GnC_Cy
Cuts at: 123 210 404 582 635 867 1193 1242 1605
Size: 87 194 178 53 232 326 49 363
Cuts at: 1605 1725 1845 1965 2085 2205 2325 2445 2565
Size: 120 120 120 120 120 120 120 120
Cuts at: 2565 2623 2749 2907 3135 3497 3505 3585 3827
Size: 58 126 158 228 362 8 80 242
Cuts at: 3827 3912 4014 4239 4328 4659 123
Size: 85 102 225 89 331 2984
Fragments arranged by size:
2984 363 362 331 326 242 232 228
225 194 178 158 126 120 120 120
120 120 120 120 120 102 89 87
85 80 58 53 49 8
EcoRI G'AATT_C
Cuts at: 4524 4524
Size: 7520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1261 4119 5539 1261
Size: 2858 1420 3242
Fragments arranged by size:
3242 2858 1420
FspI TGC'GCA
Cuts at: 5301 5301
Size: 7520
HaeII r_GCGC'y
Cuts at: 244 4954 5202 6432 7203 244
Size: 4710 248 1230 771 561
Fragments arranged by size:
4710 1230 771 561 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 556 588 634 666 667 699 883
Size: 32 412 32 46 32 1 32 184
Cuts at: 883 915 964 988 994 996 1020 1026 1534
Size: 32 49 24 6 2 24 6 508
Cuts at: 1534 1566 1626 1654 1658 1686 1746 1778 1866
Size: 32 60 28 4 28 60 32 88
Cuts at: 1866 1894 1898 1926 1986 2014 2018 2046 2106
Size: 28 4 28 60 28 4 28 60
Cuts at: 2106 2134 2138 2166 2226 2254 2258 2286 2346
Size: 28 4 28 60 28 4 28 60
Cuts at: 2346 2374 2378 2406 2466 2494 2498 2526 2586
Size: 28 4 28 60 28 4 28 60
Cuts at: 2586 2618 4228 4260 5362 5392 5394 5424 112
Size: 32 1610 32 1102 30 2 30 2208
Fragments arranged by size:
2208 1610 1102 508 412 184 88 60
60 60 60 60 60 60 60 49
46 32 32 32 32 32 32 32
32 32 30 30 28 28 28 28
28 28 28 28 28 28 28 28
28 28 24 24 6 6 4 4
4 4 4 4 4 2 2 1
HincII GTy'rAC
Cuts at: 4491 4559 4491
Size: 68 7452
KasI G'GCGC_C
Cuts at: 4950 5198 4950
Size: 248 7272
KpnI G_GTAC'C
Cuts at: 3530 4540 3530
Size: 1010 6510
MluI A'CGCG_T
Cuts at: 6953 6953
Size: 7520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1492 2847 6279 6463 1492
Size: 1355 3432 184 2549
Fragments arranged by size:
3432 2549 1355 184
MscI TGG'CCA
Cuts at: 730 2656 2713 3259 5281 730
Size: 1926 57 546 2022 2969
Fragments arranged by size:
2969 2022 1926 546 57
MslI CAynn'nnrTG
Cuts at: 1396 1702 1822 1942 2062 2182 2302 2422 2542
Size: 306 120 120 120 120 120 120 120
Cuts at: 2542 3008 3487 4210 5509 5636 1396
Size: 466 479 723 1299 127 3280
Fragments arranged by size:
3280 1299 723 479 466 306 127 120
120 120 120 120 120 120
MspA1I CmG'CkG
Cuts at: 509 1046 1372 2845 3253 3670 3841 4270 4631
Size: 537 326 1473 408 417 171 429 361
Cuts at: 4631 4945 5305 6089 6334 509
Size: 314 360 784 245 1695
Fragments arranged by size:
1695 1473 784 537 429 417 408 361
360 326 314 245 171
NaeI GCC'GGC
Cuts at: 2927 5701 2927
Size: 2774 4746
NarI GG'CG_CC
Cuts at: 4951 5199 4951
Size: 248 7272
NcoI C'CATG_G
Cuts at: 602 1391 2717 3764 4511 602
Size: 789 1326 1047 747 3611
Fragments arranged by size:
3611 1326 1047 789 747
NgoMIV G'CCGG_C
Cuts at: 2925 5699 2925
Size: 2774 4746
NspI r_CATG'y
Cuts at: 2987 4573 5073 6676 2987
Size: 1586 500 1603 3831
Fragments arranged by size:
3831 1603 1586 500
PasI CC'CwG_GG
Cuts at: 2695 3501 3915 2695
Size: 806 414 6300
Fragments arranged by size:
6300 806 414
PciI A'CATG_T
Cuts at: 6672 6672
Size: 7520
PflMI CCAn_nnn'nTGG
Cuts at: 1345 1345
Size: 7520
PfoI T'CCnGG_A
Cuts at: 968 1076 2795 968
Size: 108 1719 5693
Fragments arranged by size:
5693 1719 108
PmeI GTTT'AAAC
Cuts at: 4519 4519
Size: 7520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5966 5998 5966
Size: 32 7488
PpuMI rG'GwC_Cy
Cuts at: 635 867 1605 3135 3585 4239 635
Size: 232 738 1530 450 654 3916
Fragments arranged by size:
3916 1530 738 654 450 232
PshAI GACnn'nnGTC
Cuts at: 3108 3108
Size: 7520
PspOMI G'GGCC_C
Cuts at: 403 3504 3826 3911 403
Size: 3101 322 85 4012
Fragments arranged by size:
4012 3101 322 85
PstI C_TGCA'G
Cuts at: 675 917 987 1133 1295 1458 1575 1695 1815
Size: 242 70 146 162 163 117 120 120
Cuts at: 1815 1935 2055 2175 2295 2415 2535 2808 2850
Size: 120 120 120 120 120 120 273 42
Cuts at: 2850 3449 4567 5252 675
Size: 599 1118 685 2943
Fragments arranged by size:
2943 1118 685 599 273 242 163 162
146 120 120 120 120 120 120 120
120 117 70 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7520
PvuII CAG'CTG
Cuts at: 509 2845 3253 3841 4270 4945 5305 509
Size: 2336 408 588 429 675 360 2724
Fragments arranged by size:
2724 2336 675 588 429 408 360
RsrII CG'GwC_CG
Cuts at: 5715 5715
Size: 7520
SacI G_AGCT'C
Cuts at: 4534 4534
Size: 7520
SalI G'TCGA_C
Cuts at: 4557 4557
Size: 7520
SanDI GG'GwC_CC
Cuts at: 635 635
Size: 7520
SapI GCTCTTCn'nnn_
Cuts at: 5543 5753 5543
Size: 210 7310
SbfI CC_TGCA'GG
Cuts at: 4567 4567
Size: 7520
ScaI AGT'ACT
Cuts at: 7037 7037
Size: 7520
SexAI A'CCwGG_T
Cuts at: 2816 2816
Size: 7520
SfcI C'TryA_G
Cuts at: 33 671 913 983 1129 1291 1454 1565 1571
Size: 638 242 70 146 162 163 111 6
Cuts at: 1571 1691 1811 1931 2051 2171 2291 2411 2531
Size: 120 120 120 120 120 120 120 120
Cuts at: 2531 2789 2804 2846 3445 4563 5248 6216 6407
Size: 258 15 42 599 1118 685 968 191
Cuts at: 6407 33
Size: 1146
Fragments arranged by size:
1146 1118 968 685 638 599 258 242
191 163 162 146 120 120 120 120
120 120 120 120 111 70 42 15
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 701 3265 701
Size: 2564 4956
SfoI GGC'GCC
Cuts at: 4952 5200 4952
Size: 248 7272
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7520
SmaI CCC'GGG
Cuts at: 4333 4542 4333
Size: 209 7311
SmlI C'TyrA_G
Cuts at: 3848 4675 5590 6304 6566 3848
Size: 827 915 714 262 4802
Fragments arranged by size:
4802 915 827 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7520
SphI G_CATG'C
Cuts at: 4573 5073 4573
Size: 500 7020
StuI AGG'CCT
Cuts at: 1307 2743 2970 3077 3212 1307
Size: 1436 227 107 135 5615
Fragments arranged by size:
5615 1436 227 135 107
StyI C'CwwG_G
Cuts at: 602 1121 1391 2717 2810 2863 3429 3467 3752
Size: 519 270 1326 93 53 566 38 285
Cuts at: 3752 3764 4304 4448 4511 4654 5008 602
Size: 12 540 144 63 143 354 3114
Fragments arranged by size:
3114 1326 566 540 519 354 285 270
144 143 93 63 53 38 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 237 641 4338 5103 5236 5695 5904 237
Size: 404 3697 765 133 459 209 1853
Fragments arranged by size:
3697 1853 765 459 404 209 133
TatI w'GTAC_w
Cuts at: 1040 3381 7035 1040
Size: 2341 3654 1525
Fragments arranged by size:
3654 2341 1525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 138 170 1485 1517 2619 2651 7037 7069 138
Size: 32 1315 32 1102 32 4386 32 589
Fragments arranged by size:
4386 1315 1102 589 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1084 1149 2818 4486 5317 1084
Size: 65 1669 1668 831 3287
Fragments arranged by size:
3287 1669 1668 831 65
XbaI T'CTAG_A
Cuts at: 4551 4551
Size: 7520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2898 3486 3696 3838 5015 2898
Size: 588 210 142 1177 5403
Fragments arranged by size:
5403 1177 588 210 142
XmaI C'CCGG_G
Cuts at: 4331 4540 4331
Size: 209 7311
XmnI GAAnn'nnTTC
Cuts at: 1463 1463
Size: 7520
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BspEI BssHII BstEII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PsiI PspXI PsrI
SacII SgrAI SnaBI SrfI SspI SwaI XhoI ZraI