Restriction map for
pF1KA0358
(Circular) (Six-base) MAPSORT of: pF1KA0358.seq Check: 9608 from: 1 to: 7856
pF1KA0358 7856 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1189 3754 4776 1189
Size: 2565 1022 4269
Fragments arranged by size:
4269 2565 1022
AatII G_ACGT'C
Cuts at: 2370 3613 2370
Size: 1243 6613
AccI GT'mk_AC
Cuts at: 688 908 4894 688
Size: 220 3986 3650
Fragments arranged by size:
3986 3650 220
Acc65I G'GTAC_C
Cuts at: 4160 4872 4160
Size: 712 7144
AclI AA'CG_TT
Cuts at: 7696 7696
Size: 7856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2673 2816 2962 3548 3713 3953 4780 5681 6113
Size: 143 146 586 165 240 827 901 432
Cuts at: 6113 6466 7128 2673
Size: 353 662 3401
Fragments arranged by size:
3401 901 827 662 586 432 353 240
165 146 143
AfeI AGC'GCT
Cuts at: 4537 4537
Size: 7856
AflIII A'CryG_T
Cuts at: 1636 2502 5208 7008 7289 1636
Size: 866 2706 1800 281 2203
Fragments arranged by size:
2706 2203 1800 866 281
AhdI GACnn_n'nnGTC
Cuts at: 1503 4571 1503
Size: 3068 4788
AleI CACnn'nnGTG
Cuts at: 982 3706 982
Size: 2724 5132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3200 3200
Size: 7856
AlwNI CAG_nnn'CTG
Cuts at: 1206 2940 4758 4833 5161 6599 1206
Size: 1734 1818 75 328 1438 2463
Fragments arranged by size:
2463 1818 1734 1438 328 75
ApaLI G'TGCA_C
Cuts at: 2953 6694 2953
Size: 3741 4115
ApoI r'AATT_y
Cuts at: 1447 1522 1802 3450 4813 4860 7209 7465 1447
Size: 75 280 1648 1363 47 2349 256 1838
Fragments arranged by size:
2349 1838 1648 1363 280 256 75 47
AseI AT'TA_AT
Cuts at: 20 2057 20
Size: 2037 5819
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7856
AvaI C'yCGr_G
Cuts at: 174 280 578 752 1085 2585 2726 2806 3386
Size: 106 298 174 333 1500 141 80 580
Cuts at: 3386 3809 4876 174
Size: 423 1067 3154
Fragments arranged by size:
3154 1500 1067 580 423 333 298 174
141 106 80
AvrII C'CTAG_G
Cuts at: 3972 3972
Size: 7856
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1623 1656 2664 2697 1623
Size: 33 1008 33 6782
Fragments arranged by size:
6782 1008 33 33
BamHI G'GATC_C
Cuts at: 1769 3318 3988 4881 1769
Size: 1549 670 893 4744
Fragments arranged by size:
4744 1549 893 670
BanI G'GyrC_C
Cuts at: 170 2722 3390 4160 4298 4872 5286 5534 5569
Size: 2552 668 770 138 574 414 248 35
Cuts at: 5569 170
Size: 2457
Fragments arranged by size:
2552 2457 770 668 574 414 248 138
35
BanII G_rGCy'C
Cuts at: 514 935 2432 2523 4870 5900 514
Size: 421 1497 91 2347 1030 2470
Fragments arranged by size:
2470 2347 1497 1030 421 91
BbeI G_GCGC'C
Cuts at: 4302 5290 5538 4302
Size: 988 248 6620
Fragments arranged by size:
6620 988 248
BbsI GAAGACnn'nnnn_
Cuts at: 333 4768 333
Size: 4435 3421
BbvCI CC'TCA_GC
Cuts at: 1913 3166 1913
Size: 1253 6603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 820 854 820
Size: 34 7822
BciVI GTATCCnnnnn_n'
Cuts at: 5754 6810 7856 5754
Size: 1056 1046 5754
Fragments arranged by size:
5754 1056 1046
BclI T'GATC_A
Cuts at: 3630 4359 5376 3630
Size: 729 1017 6110
Fragments arranged by size:
6110 1017 729
BglI GCCn_nnn'nGGC
Cuts at: 731 731
Size: 7856
BglII A'GATC_T
Cuts at: 5371 7408 5371
Size: 2037 5819
BlpI GC'TnA_GC
Cuts at: 646 4968 646
Size: 4322 3534
Bme1580I G_kGCm'C
Cuts at: 825 2727 2957 3393 3817 5481 5574 6698 825
Size: 1902 230 436 424 1664 93 1124 1983
Fragments arranged by size:
1983 1902 1664 1124 436 424 230 93
BmgBI CAC'GTC
Cuts at: 632 1702 632
Size: 1070 6786
BmrI ACTGGGnnnn_n'
Cuts at: 751 874 2110 2744 3301 3388 5068 5149 5484
Size: 123 1236 634 557 87 1680 81 335
Cuts at: 5484 7628 751
Size: 2144 979
Fragments arranged by size:
2144 1680 1236 979 634 557 335 123
87 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 540 1959 2598 3861 3973 6223 540
Size: 1419 639 1263 112 2250 2173
Fragments arranged by size:
2250 2173 1419 1263 639 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 919 1021 1462 2190 3037 3743 386
Size: 533 102 441 728 847 706 4499
Fragments arranged by size:
4499 847 728 706 533 441 102
Bpu10I CC'TnA_GC
Cuts at: 292 1913 3046 3166 292
Size: 1621 1133 120 4982
Fragments arranged by size:
4982 1621 1133 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 2568 3136 3663 3771 5032 5911 6625 6923
Size: 1889 568 527 108 1261 879 714 298
Cuts at: 6923 679
Size: 1612
Fragments arranged by size:
1889 1612 1261 879 714 568 527 298
108
BsaI GGTCTCn'nnnn_
Cuts at: 3721 3832 4018 4612 7560 3721
Size: 111 186 594 2948 4017
Fragments arranged by size:
4017 2948 594 186 111
BsaAI yAC'GTr
Cuts at: 5209 5209
Size: 7856
BsaBI GATnn'nnATC
Cuts at: 6 4208 5370 7407 6
Size: 4202 1162 2037 455
Fragments arranged by size:
4202 2037 1162 455
BsaHI Gr'CG_yC
Cuts at: 1028 2309 2367 3610 4299 5287 5535 6237 7733
Size: 1281 58 1243 689 988 248 702 1496
Cuts at: 7733 1028
Size: 1151
Fragments arranged by size:
1496 1281 1243 1151 988 702 689 248
58
BsaWI w'CCGG_w
Cuts at: 4184 5045 5566 6244 6655 6802 7076 4184
Size: 861 521 678 411 147 274 4964
Fragments arranged by size:
4964 861 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1933 1963 3559 3589 4228 4258 1933
Size: 30 1596 30 639 30 5531
Fragments arranged by size:
5531 1596 639 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 566 823 2004 3139 3580 566
Size: 257 1181 1135 441 4842
Fragments arranged by size:
4842 1181 1135 441 257
BseYI C'CCAG_C
Cuts at: 1424 1904 2236 2338 3331 4622 5281 6039 6704
Size: 480 332 102 993 1291 659 758 665
Cuts at: 6704 7112 1424
Size: 408 2168
Fragments arranged by size:
2168 1291 993 758 665 659 480 408
332 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 3088 4216 4771 982
Size: 2106 1128 555 4067
Fragments arranged by size:
4067 2106 1128 555
BsiEI CG_ry'CG
Cuts at: 104 5398 5444 6674 104
Size: 5294 46 1230 1286
Fragments arranged by size:
5294 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 514 647 935 1067 2957 4870 5648 5838 6698
Size: 133 288 132 1890 1913 778 190 860
Cuts at: 6698 514
Size: 1672
Fragments arranged by size:
1913 1890 1672 860 778 288 190 133
132
BsmI GAATG_Cn'
Cuts at: 1052 1120 1052
Size: 68 7788
BsmBI CGTCTCn'nnnn_
Cuts at: 894 2316 2616 894
Size: 1422 300 6134
Fragments arranged by size:
6134 1422 300
Bsp1286I G_dGCh'C
Cuts at: 514 647 825 935 1067 2432 2523 2727 2957
Size: 133 178 110 132 1365 91 204 230
Cuts at: 2957 3393 3817 4870 5481 5574 5648 5838 5900
Size: 436 424 1053 611 93 74 190 62
Cuts at: 5900 6698 514
Size: 798 1672
Fragments arranged by size:
1672 1365 1053 798 611 436 424 230
204 190 178 133 132 110 93 91
74 62
BspHI T'CATG_A
Cuts at: 1452 6288 1452
Size: 4836 3020
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1057 1189 1508 2277 3754 4776 4906 5120 5422
Size: 132 319 769 1477 1022 130 214 302
Cuts at: 5422 5803 1057
Size: 381 3110
Fragments arranged by size:
3110 1477 1022 769 381 319 302 214
132 130
BsrBI CCG'CTC
Cuts at: 50 404 611 1938 6148 6202 7686 7851 50
Size: 354 207 1327 4210 54 1484 165 55
Fragments arranged by size:
4210 1484 1327 354 207 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1136 3765 5768 1136
Size: 2629 2003 3224
Fragments arranged by size:
3224 2629 2003
BsrFI r'CCGG_y
Cuts at: 2399 2800 3008 4412 5854 6035 2399
Size: 401 208 1404 1442 181 4220
Fragments arranged by size:
4220 1442 1404 401 208 181
BsrGI T'GTAC_A
Cuts at: 3128 3157 4331 3128
Size: 29 1174 6653
Fragments arranged by size:
6653 1174 29
BssHII G'CGCG_C
Cuts at: 4689 4689
Size: 7856
BssSI C'ACGA_G
Cuts at: 6127 6835 6127
Size: 708 7148
BstAPI GCAn_nnn'nTGC
Cuts at: 2961 3397 5233 2961
Size: 436 1836 5584
Fragments arranged by size:
5584 1836 436
BstBI TT'CG_AA
Cuts at: 6217 6217
Size: 7856
BstEII G'GTnAC_C
Cuts at: 4788 4788
Size: 7856
BstXI CCAn_nnnn'nTGG
Cuts at: 1185 2118 2980 1185
Size: 933 862 6061
Fragments arranged by size:
6061 933 862
BstYI r'GATC_y
Cuts at: 1 782 857 1299 1466 1769 3318 3988 4881
Size: 781 75 442 167 303 1549 670 893
Cuts at: 4881 5348 5371 5706 5952 6258 6270 6356 7408
Size: 467 23 335 246 306 12 86 1052
Cuts at: 7408 1
Size: 449
Fragments arranged by size:
1549 1052 893 781 670 467 449 442
335 306 303 246 167 86 75 23
12
BstZ17I GTA'TAC
Cuts at: 689 689
Size: 7856
Bsu36I CC'TnA_GG
Cuts at: 2450 3416 2450
Size: 966 6890
BtgI C'CryG_G
Cuts at: 107 456 1583 2250 3704 107
Size: 349 1127 667 1454 4259
Fragments arranged by size:
4259 1454 1127 667 349
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4505 5806 5987 6150 4505
Size: 1301 181 163 6211
Fragments arranged by size:
6211 1301 181 163
BtsI GCAGTG_nn'
Cuts at: 186 994 1980 2197 2707 3795 5099 186
Size: 808 986 217 510 1088 1304 2943
Fragments arranged by size:
2943 1304 1088 986 808 510 217
ClaI AT'CG_AT
Cuts at: 864 864
Size: 7856
DraI TTT'AAA
Cuts at: 1391 3440 4855 1391
Size: 2049 1415 4392
Fragments arranged by size:
4392 2049 1415
DraIII CAC_nnn'GTG
Cuts at: 776 1071 2961 3397 776
Size: 295 1890 436 5235
Fragments arranged by size:
5235 1890 436 295
DrdI GACnn_nn'nnGTC
Cuts at: 367 916 5562 6906 367
Size: 549 4646 1344 1317
Fragments arranged by size:
4646 1344 1317 549
EaeI y'GGCC_r
Cuts at: 1403 1733 2970 3209 4220 5441 5615 6006 6033
Size: 330 1237 239 1011 1221 174 391 27
Cuts at: 6033 7378 1403
Size: 1345 1881
Fragments arranged by size:
1881 1345 1237 1221 1011 391 330 239
174 27
EagI C'GGCC_G
Cuts at: 5441 5441
Size: 7856
EarI CTCTTCn'nnn_
Cuts at: 488 962 2010 2150 3271 5879 6089 488
Size: 474 1048 140 1121 2608 210 2255
Fragments arranged by size:
2608 2255 1121 1048 474 210 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 795 1593 6064 6806 6952 7666 795
Size: 798 4471 742 146 714 985
Fragments arranged by size:
4471 985 798 742 714 146
EcoICRI GAG'CTC
Cuts at: 512 933 4868 512
Size: 421 3935 3500
Fragments arranged by size:
3935 3500 421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 919 1021 1462 2190 2673 2816 2962 3037
Size: 533 102 441 728 483 143 146 75
Cuts at: 3037 3548 3713 3743 3953 4780 5681 6113 6466
Size: 511 165 30 210 827 901 432 353
Cuts at: 6466 7128 386
Size: 662 1114
Fragments arranged by size:
1114 901 827 728 662 533 511 483
441 432 353 210 165 146 143 102
75 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2944 4783 2944
Size: 1839 6017
EcoO109I rG'GnC_Cy
Cuts at: 452 918 1917 3342 3382 3412 4138 4755 4995
Size: 466 999 1425 40 30 726 617 240
Cuts at: 4995 452
Size: 3313
Fragments arranged by size:
3313 1425 999 726 617 466 240 40
30
EcoRI G'AATT_C
Cuts at: 1522 1802 4860 1522
Size: 280 3058 4518
Fragments arranged by size:
4518 3058 280
EcoRV GAT'ATC
Cuts at: 4470 4470
Size: 7856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1416 2024 5875 1416
Size: 608 3851 3397
Fragments arranged by size:
3851 3397 608
FspI TGC'GCA
Cuts at: 5637 5637
Size: 7856
HaeII r_GCGC'y
Cuts at: 702 726 4194 4302 4539 5290 5538 6768 7539
Size: 24 3468 108 237 751 248 1230 771
Cuts at: 7539 702
Size: 1019
Fragments arranged by size:
3468 1230 1019 771 751 248 237 108
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 368 400 799 831 896 928 939
Size: 32 20 32 399 32 65 32 11
Cuts at: 939 971 1489 1521 1933 1965 1987 2019 2560
Size: 32 518 32 412 32 22 32 541
Cuts at: 2560 2592 2833 2865 3026 3058 3559 3591 3615
Size: 32 241 32 161 32 501 32 24
Cuts at: 3615 3622 3647 3654 3973 4005 4373 4405 4557
Size: 7 25 7 319 32 368 32 152
Cuts at: 4557 4589 5698 5728 5730 5760 316
Size: 32 1109 30 2 30 2412
Fragments arranged by size:
2412 1109 541 518 501 412 399 368
319 241 161 152 65 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 25 24
22 20 11 7 7 2
HincII GTy'rAC
Cuts at: 393 4895 393
Size: 4502 3354
HindIII A'AGCT_T
Cuts at: 3506 3506
Size: 7856
HpaI GTT'AAC
Cuts at: 393 393
Size: 7856
KasI G'GCGC_C
Cuts at: 4298 5286 5534 4298
Size: 988 248 6620
Fragments arranged by size:
6620 988 248
KpnI G_GTAC'C
Cuts at: 4164 4876 4164
Size: 712 7144
MluI A'CGCG_T
Cuts at: 1636 7289 1636
Size: 5653 2203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2639 3100 3310 4088 6615 6799 1240
Size: 1399 461 210 778 2527 184 2297
Fragments arranged by size:
2527 2297 1399 778 461 210 184
MscI TGG'CCA
Cuts at: 1405 1735 3211 4222 5617 1405
Size: 330 1476 1011 1395 3644
Fragments arranged by size:
3644 1476 1395 1011 330
MslI CAynn'nnrTG
Cuts at: 982 1111 1183 2507 3649 3706 4483 4561 5845
Size: 129 72 1324 1142 57 777 78 1284
Cuts at: 5845 5972 982
Size: 127 2866
Fragments arranged by size:
2866 1324 1284 1142 777 129 127 78
72 57
MspA1I CmG'CkG
Cuts at: 306 1170 1203 1866 1908 3030 3661 4365 4967
Size: 864 33 663 42 1122 631 704 602
Cuts at: 4967 5281 5641 6425 6670 306
Size: 314 360 784 245 1492
Fragments arranged by size:
1492 1122 864 784 704 663 631 602
360 314 245 42 33
NaeI GCC'GGC
Cuts at: 2401 2802 3010 4414 6037 2401
Size: 401 208 1404 1623 4220
Fragments arranged by size:
4220 1623 1404 401 208
NarI GG'CG_CC
Cuts at: 4299 5287 5535 4299
Size: 988 248 6620
Fragments arranged by size:
6620 988 248
NcoI C'CATG_G
Cuts at: 107 1583 107
Size: 1476 6380
NgoMIV G'CCGG_C
Cuts at: 2399 2800 3008 4412 6035 2399
Size: 401 208 1404 1623 4220
Fragments arranged by size:
4220 1623 1404 401 208
NspI r_CATG'y
Cuts at: 2095 2506 4254 4447 4909 5409 7012 2095
Size: 411 1748 193 462 500 1603 2939
Fragments arranged by size:
2939 1748 1603 500 462 411 193
PasI CC'CwG_GG
Cuts at: 2901 2901
Size: 7856
PciI A'CATG_T
Cuts at: 2502 7008 2502
Size: 4506 3350
PfoI T'CCnGG_A
Cuts at: 3101 3101
Size: 7856
PmeI GTTT'AAAC
Cuts at: 4855 4855
Size: 7856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6302 6334 6302
Size: 32 7824
PpuMI rG'GwC_Cy
Cuts at: 452 918 3342 3382 3412 4138 4755 452
Size: 466 2424 40 30 726 617 3553
Fragments arranged by size:
3553 2424 726 617 466 40 30
PshAI GACnn'nnGTC
Cuts at: 1031 2366 4136 1031
Size: 1335 1770 4751
Fragments arranged by size:
4751 1770 1335
PspXI vC'TCGA_Gb
Cuts at: 752 752
Size: 7856
PstI C_TGCA'G
Cuts at: 536 1505 1619 1871 2138 4787 4903 5588 536
Size: 969 114 252 267 2649 116 685 2804
Fragments arranged by size:
2804 2649 969 685 267 252 116 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7856
PvuII CAG'CTG
Cuts at: 1203 1866 1908 3030 3661 4365 5281 5641 1203
Size: 663 42 1122 631 704 916 360 3418
Fragments arranged by size:
3418 1122 916 704 663 631 360 42
RsrII CG'GwC_CG
Cuts at: 6051 6051
Size: 7856
SacI G_AGCT'C
Cuts at: 514 935 4870 514
Size: 421 3935 3500
Fragments arranged by size:
3935 3500 421
SalI G'TCGA_C
Cuts at: 4893 4893
Size: 7856
SanDI GG'GwC_CC
Cuts at: 452 3342 4138 452
Size: 2890 796 4170
Fragments arranged by size:
4170 2890 796
SapI GCTCTTCn'nnn_
Cuts at: 962 2010 2150 5879 6089 962
Size: 1048 140 3729 210 2729
Fragments arranged by size:
3729 2729 1048 210 140
SbfI CC_TGCA'GG
Cuts at: 4903 4903
Size: 7856
ScaI AGT'ACT
Cuts at: 624 1756 7373 624
Size: 1132 5617 1107
Fragments arranged by size:
5617 1132 1107
SexAI A'CCwGG_T
Cuts at: 3774 3774
Size: 7856
SfcI C'TryA_G
Cuts at: 33 532 714 1078 1501 1615 1867 2134 3252
Size: 499 182 364 423 114 252 267 1118
Cuts at: 3252 4783 4899 5584 6552 6743 33
Size: 1531 116 685 968 191 1146
Fragments arranged by size:
1531 1146 1118 968 685 499 423 364
267 252 191 182 116 114
SfoI GGC'GCC
Cuts at: 4300 5288 5536 4300
Size: 988 248 6620
Fragments arranged by size:
6620 988 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7856
SgrAI Cr'CCGG_yG
Cuts at: 2399 2399
Size: 7856
SmaI CCC'GGG
Cuts at: 2808 4878 2808
Size: 2070 5786
SmlI C'TyrA_G
Cuts at: 694 752 2547 3151 3678 3750 5011 5926 6640
Size: 58 1795 604 527 72 1261 915 714
Cuts at: 6640 6902 694
Size: 262 1648
Fragments arranged by size:
1795 1648 1261 915 714 604 527 262
72 58
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7856
SphI G_CATG'C
Cuts at: 4909 5409 4909
Size: 500 7356
StuI AGG'CCT
Cuts at: 853 1399 1488 3525 853
Size: 546 89 2037 5184
Fragments arranged by size:
5184 2037 546 89
StyI C'CwwG_G
Cuts at: 107 224 596 1007 1400 1583 2019 3552 3732
Size: 117 372 411 393 183 436 1533 180
Cuts at: 3732 3972 4170 4990 5344 107
Size: 240 198 820 354 2619
Fragments arranged by size:
2619 1533 820 436 411 393 372 354
240 198 183 180 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1438 3190 4723 5439 5572 6031 6240 1438
Size: 1752 1533 716 133 459 209 3054
Fragments arranged by size:
3054 1752 1533 716 459 209 133
TatI w'GTAC_w
Cuts at: 622 1754 3128 3157 4331 4661 7371 622
Size: 1132 1374 29 1174 330 2710 1107
Fragments arranged by size:
2710 1374 1174 1132 1107 330 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 228 260 760 792 7373 7405 228
Size: 32 500 32 6581 32 679
Fragments arranged by size:
6581 679 500 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2029 3640 5653 2029
Size: 1611 2013 4232
Fragments arranged by size:
4232 2013 1611
XbaI T'CTAG_A
Cuts at: 4887 4887
Size: 7856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1113 1437 1581 2506 5351 1113
Size: 324 144 925 2845 3618
Fragments arranged by size:
3618 2845 925 324 144
XhoI C'TCGA_G
Cuts at: 752 752
Size: 7856
XmaI C'CCGG_G
Cuts at: 2806 4876 2806
Size: 2070 5786
XmnI GAAnn'nnTTC
Cuts at: 124 1488 124
Size: 1364 6492
ZraI GAC'GTC
Cuts at: 2368 3611 2368
Size: 1243 6613
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AloI ApaI AscI BfrBI BmtI BsiWI
BspEI CspCI FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PsiI PspOMI PsrI
SacII SfiI SnaBI SrfI SspI SwaI