Restriction map for
pF1KA0339
(Circular) (Six-base) MAPSORT of: pF1KA0339.seq Check: 833 from: 1 to: 8234
pF1KA0339 8234 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2363 3525 2363
Size: 1162 7072
AatII G_ACGT'C
Cuts at: 2916 2916
Size: 8234
AccI GT'mk_AC
Cuts at: 5272 5272
Size: 8234
Acc65I G'GTAC_C
Cuts at: 4750 5250 4750
Size: 500 7734
AclI AA'CG_TT
Cuts at: 5228 8074 5228
Size: 2846 5388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 406 457 1043 1615 2692 3314 4074 6059
Size: 175 51 586 572 1077 622 760 1985
Cuts at: 6059 6491 6844 7506 231
Size: 432 353 662 959
Fragments arranged by size:
1985 1077 959 760 662 622 586 572
432 353 175 51
AfeI AGC'GCT
Cuts at: 1318 1606 4945 1318
Size: 288 3339 4607
Fragments arranged by size:
4607 3339 288
AflIII A'CryG_T
Cuts at: 926 4643 5586 7386 7667 926
Size: 3717 943 1800 281 1493
Fragments arranged by size:
3717 1800 1493 943 281
AleI CACnn'nnGTG
Cuts at: 248 805 248
Size: 557 7677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1603 4028 1603
Size: 2425 5809
AlwNI CAG_nnn'CTG
Cuts at: 739 1108 1426 3379 4167 5539 6977 739
Size: 369 318 1953 788 1372 1438 1996
Fragments arranged by size:
1996 1953 1438 1372 788 369 318
ApaI G_GGCC'C
Cuts at: 1454 1721 2769 3527 4565 1454
Size: 267 1048 758 1038 5123
Fragments arranged by size:
5123 1048 1038 758 267
ApaLI G'TGCA_C
Cuts at: 7072 7072
Size: 8234
ApoI r'AATT_y
Cuts at: 2813 5238 7587 7843 2813
Size: 2425 2349 256 3204
Fragments arranged by size:
3204 2425 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8234
AvaI C'yCGr_G
Cuts at: 144 542 726 932 1351 1793 2400 3707 3772
Size: 398 184 206 419 442 607 1307 65
Cuts at: 3772 3785 4123 4404 4565 5254 144
Size: 13 338 281 161 689 3124
Fragments arranged by size:
3124 1307 689 607 442 419 398 338
281 206 184 161 65 13
AvrII C'CTAG_G
Cuts at: 3973 3973
Size: 8234
BamHI G'GATC_C
Cuts at: 180 4835 5259 180
Size: 4655 424 3155
Fragments arranged by size:
4655 3155 424
BanI G'GyrC_C
Cuts at: 547 796 826 1695 1761 2473 3387 3633 4267
Size: 249 30 869 66 712 914 246 634
Cuts at: 4267 4304 4750 5250 5664 5912 5947 547
Size: 37 446 500 414 248 35 2834
Fragments arranged by size:
2834 914 869 712 634 500 446 414
249 248 246 66 37 35 30
BanII G_rGCy'C
Cuts at: 1293 1358 1454 1721 2102 2121 2381 2769 3527
Size: 65 96 267 381 19 260 388 758
Cuts at: 3527 3650 4154 4565 5221 5248 6278 1293
Size: 123 504 411 656 27 1030 3249
Fragments arranged by size:
3249 1030 758 656 504 411 388 381
267 260 123 96 65 27 19
BbeI G_GCGC'C
Cuts at: 551 4271 4308 5668 5916 551
Size: 3720 37 1360 248 2869
Fragments arranged by size:
3720 2869 1360 248 37
BbsI GAAGACnn'nnnn_
Cuts at: 287 2668 2879 3140 3938 5180 287
Size: 2381 211 261 798 1242 3341
Fragments arranged by size:
3341 2381 1242 798 261 211
BbvCI CC'TCA_GC
Cuts at: 1910 2214 2861 3333 4139 1910
Size: 304 647 472 806 6005
Fragments arranged by size:
6005 806 647 472 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4683 4717 4683
Size: 34 8200
BciVI GTATCCnnnnn_n'
Cuts at: 3084 6132 7188 8234 3084
Size: 3048 1056 1046 3084
Fragments arranged by size:
3084 3048 1056 1046
BclI T'GATC_A
Cuts at: 5754 5754
Size: 8234
BglI GCCn_nnn'nGGC
Cuts at: 1081 1362 1399 3883 1081
Size: 281 37 2484 5432
Fragments arranged by size:
5432 2484 281 37
BglII A'GATC_T
Cuts at: 1859 5749 7786 1859
Size: 3890 2037 2307
Fragments arranged by size:
3890 2307 2037
BlpI GC'TnA_GC
Cuts at: 1313 3842 3985 4584 4738 5346 1313
Size: 2529 143 599 154 608 4201
Fragments arranged by size:
4201 2529 608 599 154 143
Bme1580I G_kGCm'C
Cuts at: 689 814 1454 1550 1721 2722 2769 3368 3527
Size: 125 640 96 171 1001 47 599 159
Cuts at: 3527 4073 4565 4651 5859 5952 7076 689
Size: 546 492 86 1208 93 1124 1847
Fragments arranged by size:
1847 1208 1124 1001 640 599 546 492
171 159 125 96 93 86 47
BmgBI CAC'GTC
Cuts at: 929 4644 929
Size: 3715 4519
BmrI ACTGGGnnnn_n'
Cuts at: 331 701 2712 3355 3412 3482 4509 4645 5446
Size: 370 2011 643 57 70 1027 136 801
Cuts at: 5446 5527 5862 8006 331
Size: 81 335 2144 559
Fragments arranged by size:
2144 2011 1027 801 643 559 370 335
136 81 70 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1623 3042 3306 3576 3838 4251 6601 1623
Size: 1419 264 270 262 413 2350 3256
Fragments arranged by size:
3256 2350 1419 413 270 264 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 840 954 1307 1403 1865 2921 3434 3638 3827
Size: 114 353 96 462 1056 513 204 189
Cuts at: 3827 4021 4135 4690 840
Size: 194 114 555 4384
Fragments arranged by size:
4384 1056 555 513 462 353 204 194
189 114 114 96
Bpu10I CC'TnA_GC
Cuts at: 1910 2214 2861 3333 4139 1910
Size: 304 647 472 806 6005
Fragments arranged by size:
6005 806 647 472 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4780 5410 6289 7003 7301 4780
Size: 630 879 714 298 5713
Fragments arranged by size:
5713 879 714 630 298
BsaI GGTCTCn'nnnn_
Cuts at: 421 1522 2525 7938 421
Size: 1101 1003 5413 717
Fragments arranged by size:
5413 1101 1003 717
BsaAI yAC'GTr
Cuts at: 4445 4899 4950 5587 4445
Size: 454 51 637 7092
Fragments arranged by size:
7092 637 454 51
BsaBI GATnn'nnATC
Cuts at: 6 3071 4381 4888 5107 5748 7785 6
Size: 3065 1310 507 219 641 2037 455
Fragments arranged by size:
3065 2037 1310 641 507 455 219
BsaHI Gr'CG_yC
Cuts at: 548 1903 1963 2913 3445 4268 4305 5665 5913
Size: 1355 60 950 532 823 37 1360 248
Cuts at: 5913 6615 8111 548
Size: 702 1496 671
Fragments arranged by size:
1496 1360 1355 950 823 702 671 532
248 60 37
BsaWI w'CCGG_w
Cuts at: 4274 4802 5423 5944 6622 7033 7180 7454 4274
Size: 528 621 521 678 411 147 274 5054
Fragments arranged by size:
5054 678 621 528 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 1716 1746 4216 4246 565
Size: 30 1121 30 2470 30 4553
Fragments arranged by size:
4553 2470 1121 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1122 1140 1158 1460 1654 1657 1937 1940 1996
Size: 18 18 302 194 3 280 3 56
Cuts at: 1996 2045 2379 2382 2422 2614 2662 3015 3019
Size: 49 334 3 40 192 48 353 4
Cuts at: 3019 3055 3219 3228 3231 3234 3237 3252 3279
Size: 36 164 9 3 3 3 15 27
Cuts at: 3279 3325 3490 3790 3803 3850 4162 4198 4210
Size: 46 165 300 13 47 312 36 12
Cuts at: 4210 4237 4240 4243 5155 1122
Size: 27 3 3 912 4201
Fragments arranged by size:
4201 912 353 334 312 302 300 280
194 192 165 164 56 49 48 47
46 40 36 36 27 27 18 18
15 13 12 9 4 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 608 789 1206 1357 1453 1491 1518 1556 1740
Size: 181 417 151 96 38 27 38 184
Cuts at: 1740 3520 3687 4098 5659 6417 7082 7490 608
Size: 1780 167 411 1561 758 665 408 1352
Fragments arranged by size:
1780 1561 1352 758 665 417 411 408
184 181 167 151 96 38 38 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 1707 4972 5037 752
Size: 955 3265 65 3949
Fragments arranged by size:
3949 3265 955 65
BsiEI CG_ry'CG
Cuts at: 104 749 1875 5776 5822 7052 104
Size: 645 1126 3901 46 1230 1286
Fragments arranged by size:
3901 1286 1230 1126 645 46
BsiHKAI G_wGCw'C
Cuts at: 530 2102 2492 4007 4574 5248 6026 6216 7076
Size: 1572 390 1515 567 674 778 190 860
Cuts at: 7076 530
Size: 1688
Fragments arranged by size:
1688 1572 1515 860 778 674 567 390
190
BsiWI C'GTAC_G
Cuts at: 496 4445 496
Size: 3949 4285
BsmBI CGTCTCn'nnnn_
Cuts at: 736 935 1841 3452 736
Size: 199 906 1611 5518
Fragments arranged by size:
5518 1611 906 199
Bsp1286I G_dGCh'C
Cuts at: 530 689 814 1293 1358 1454 1550 1721 2102
Size: 159 125 479 65 96 96 171 381
Cuts at: 2102 2121 2381 2492 2722 2769 3368 3527 3650
Size: 19 260 111 230 47 599 159 123
Cuts at: 3650 4007 4073 4154 4565 4574 4651 5221 5248
Size: 357 66 81 411 9 77 570 27
Cuts at: 5248 5859 5952 6026 6216 6278 7076 530
Size: 611 93 74 190 62 798 1688
Fragments arranged by size:
1688 798 611 599 570 479 411 381
357 260 230 190 171 159 159 125
123 111 96 96 93 81 77 74
66 65 62 47 27 19 9
BspEI T'CCGG_A
Cuts at: 4274 4802 4274
Size: 528 7706
BspHI T'CATG_A
Cuts at: 6666 6666
Size: 8234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1352 2004 2363 3525 3743 4442 4784 5284 5498
Size: 652 359 1162 218 699 342 500 214
Cuts at: 5498 5800 6181 1352
Size: 302 381 3405
Fragments arranged by size:
3405 1162 699 652 500 381 359 342
302 218 214
BsrBI CCG'CTC
Cuts at: 50 764 1508 1775 2714 3315 3720 4292 4452
Size: 714 744 267 939 601 405 572 160
Cuts at: 4452 6526 6580 8064 8229 50
Size: 2074 54 1484 165 55
Fragments arranged by size:
2074 1484 939 744 714 601 572 405
267 165 160 55 54
BsrDI GCAATG_nn'
Cuts at: 4868 6146 4868
Size: 1278 6956
BsrFI r'CCGG_y
Cuts at: 1361 1382 4715 6232 6413 1361
Size: 21 3333 1517 181 3182
Fragments arranged by size:
3333 3182 1517 181 21
BssSI C'ACGA_G
Cuts at: 2695 4840 6505 7213 2695
Size: 2145 1665 708 3716
Fragments arranged by size:
3716 2145 1665 708
BstAPI GCAn_nnn'nTGC
Cuts at: 833 1108 3460 4167 5611 833
Size: 275 2352 707 1444 3456
Fragments arranged by size:
3456 2352 1444 707 275
BstBI TT'CG_AA
Cuts at: 6595 6595
Size: 8234
BstXI CCAn_nnnn'nTGG
Cuts at: 807 2367 3630 5133 807
Size: 1560 1263 1503 3908
Fragments arranged by size:
3908 1560 1503 1263
BstYI r'GATC_y
Cuts at: 1 180 479 1859 4835 5183 5259 5726 5749
Size: 179 299 1380 2976 348 76 467 23
Cuts at: 5749 6084 6330 6636 6648 6734 7786 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2976 1380 1052 467 449 348 335 306
299 246 179 86 76 23 12
BtgI C'CryG_G
Cuts at: 107 1079 2477 3470 4859 107
Size: 972 1398 993 1389 3482
Fragments arranged by size:
3482 1398 1389 993 972
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1071 4271 6184 6365 6528 1071
Size: 3200 1913 181 163 2777
Fragments arranged by size:
3200 2777 1913 181 163
BtsI GCAGTG_nn'
Cuts at: 800 1097 1106 4368 5046 5477 800
Size: 297 9 3262 678 431 3557
Fragments arranged by size:
3557 3262 678 431 297 9
ClaI AT'CG_AT
Cuts at: 5006 5006
Size: 8234
DraI TTT'AAA
Cuts at: 5233 5233
Size: 8234
DraIII CAC_nnn'GTG
Cuts at: 4363 5018 4363
Size: 655 7579
DrdI GACnn_nn'nnGTC
Cuts at: 3114 3868 4885 5940 7284 3114
Size: 754 1017 1055 1344 4064
Fragments arranged by size:
4064 1344 1055 1017 754
EaeI y'GGCC_r
Cuts at: 746 888 1082 2627 3316 3875 4019 4824 4925
Size: 142 194 1545 689 559 144 805 101
Cuts at: 4925 5030 5819 5993 6384 6411 7756 746
Size: 105 789 174 391 27 1345 1224
Fragments arranged by size:
1545 1345 1224 805 789 689 559 391
194 174 144 142 105 101 27
EagI C'GGCC_G
Cuts at: 746 5819 746
Size: 5073 3161
EarI CTCTTCn'nnn_
Cuts at: 464 535 771 1131 1167 1643 1845 2509 2939
Size: 71 236 360 36 476 202 664 430
Cuts at: 2939 3225 3276 3296 3302 3567 3827 4093 4187
Size: 286 51 20 6 265 260 266 94
Cuts at: 4187 4202 4214 6257 6467 464
Size: 15 12 2043 210 2231
Fragments arranged by size:
2231 2043 664 476 430 360 286 266
265 260 236 210 202 94 71 51
36 20 15 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 174 1110 1910 4159 4307 4746 6442 7184 7330
Size: 936 800 2249 148 439 1696 742 146
Cuts at: 7330 8044 174
Size: 714 364
Fragments arranged by size:
2249 1696 936 800 742 714 439 364
148 146
EcoICRI GAG'CTC
Cuts at: 2100 5246 2100
Size: 3146 5088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 406 457 840 954 1043 1307 1403 1615
Size: 175 51 383 114 89 264 96 212
Cuts at: 1615 1865 2692 2921 3314 3434 3638 3827 4021
Size: 250 827 229 393 120 204 189 194
Cuts at: 4021 4074 4135 4690 6059 6491 6844 7506 231
Size: 53 61 555 1369 432 353 662 959
Fragments arranged by size:
1369 959 827 662 555 432 393 383
353 264 250 229 212 204 194 189
175 120 114 96 89 61 53 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1430 3826 1430
Size: 2396 5838
EcoO109I rG'GnC_Cy
Cuts at: 141 702 1450 1718 2234 2765 2766 3466 3524
Size: 561 748 268 516 531 1 700 58
Cuts at: 3524 3977 4110 4119 4562 5373 141
Size: 453 133 9 443 811 3002
Fragments arranged by size:
3002 811 748 700 561 531 516 453
443 268 133 58 9 1
EcoRI G'AATT_C
Cuts at: 5238 5238
Size: 8234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1635 6253 1635
Size: 4618 3616
FspI TGC'GCA
Cuts at: 205 6015 205
Size: 5810 2424
HaeII r_GCGC'y
Cuts at: 551 1242 1320 1608 3972 4271 4308 4716 4745
Size: 691 78 288 2364 299 37 408 29
Cuts at: 4745 4947 5668 5916 7146 7917 551
Size: 202 721 248 1230 771 868
Fragments arranged by size:
2364 1230 868 771 721 691 408 299
288 248 202 78 37 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 862 894 1893 1925 2154 2186 2829 2861 2995
Size: 32 999 32 229 32 643 32 134
Cuts at: 2995 3027 3181 3213 3736 3766 3768 3798 4216
Size: 32 154 32 523 30 2 30 418
Cuts at: 4216 4248 4702 4734 5175 5207 6076 6106 6108
Size: 32 454 32 441 32 869 30 2
Cuts at: 6108 6138 862
Size: 30 2958
Fragments arranged by size:
2958 999 869 643 523 454 441 418
229 154 134 32 32 32 32 32
32 32 32 32 30 30 30 30
2 2
HincII GTy'rAC
Cuts at: 255 2168 5273 255
Size: 1913 3105 3216
Fragments arranged by size:
3216 3105 1913
HindIII A'AGCT_T
Cuts at: 849 849
Size: 8234
HpaI GTT'AAC
Cuts at: 2168 2168
Size: 8234
KasI G'GCGC_C
Cuts at: 547 4267 4304 5664 5912 547
Size: 3720 37 1360 248 2869
Fragments arranged by size:
3720 2869 1360 248 37
KpnI G_GTAC'C
Cuts at: 4754 5254 4754
Size: 500 7734
MluI A'CGCG_T
Cuts at: 7667 7667
Size: 8234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1889 3098 6993 7177 1889
Size: 1209 3895 184 2946
Fragments arranged by size:
3895 2946 1209 184
MscI TGG'CCA
Cuts at: 890 5032 5995 890
Size: 4142 963 3129
Fragments arranged by size:
4142 3129 963
MslI CAynn'nnrTG
Cuts at: 248 805 1585 2075 2365 3628 4010 5131 6223
Size: 557 780 490 290 1263 382 1121 1092
Cuts at: 6223 6350 248
Size: 127 2132
Fragments arranged by size:
2132 1263 1121 1092 780 557 490 382
290 127
MspA1I CmG'CkG
Cuts at: 781 1312 2184 2218 4102 4179 4668 4728 5345
Size: 531 872 34 1884 77 489 60 617
Cuts at: 5345 5659 6019 6803 7048 781
Size: 314 360 784 245 1967
Fragments arranged by size:
1967 1884 872 784 617 531 489 360
314 245 77 60 34
NaeI GCC'GGC
Cuts at: 1363 6415 1363
Size: 5052 3182
NarI GG'CG_CC
Cuts at: 548 4268 4305 5665 5913 548
Size: 3720 37 1360 248 2869
Fragments arranged by size:
3720 2869 1360 248 37
NcoI C'CATG_G
Cuts at: 107 4859 107
Size: 4752 3482
NgoMIV G'CCGG_C
Cuts at: 1361 6413 1361
Size: 5052 3182
NspI r_CATG'y
Cuts at: 4935 5287 5787 7390 4935
Size: 352 500 1603 5779
Fragments arranged by size:
5779 1603 500 352
PasI CC'CwG_GG
Cuts at: 2426 2961 2426
Size: 535 7699
PciI A'CATG_T
Cuts at: 7386 7386
Size: 8234
PflMI CCAn_nnn'nTGG
Cuts at: 1426 3520 1426
Size: 2094 6140
PfoI T'CCnGG_A
Cuts at: 2504 3998 4112 2504
Size: 1494 114 6626
Fragments arranged by size:
6626 1494 114
PmeI GTTT'AAAC
Cuts at: 5233 5233
Size: 8234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1457 1489 6680 6712 1457
Size: 32 5191 32 2979
Fragments arranged by size:
5191 2979 32 32
PpuMI rG'GwC_Cy
Cuts at: 4110 4110
Size: 8234
PspOMI G'GGCC_C
Cuts at: 1450 1717 2765 3523 4561 1450
Size: 267 1048 758 1038 5123
Fragments arranged by size:
5123 1048 1038 758 267
PstI C_TGCA'G
Cuts at: 1104 3465 4172 4463 5281 5966 1104
Size: 2361 707 291 818 685 3372
Fragments arranged by size:
3372 2361 818 707 685 291
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8234
PvuII CAG'CTG
Cuts at: 781 1312 2184 2218 4102 4668 5659 6019 781
Size: 531 872 34 1884 566 991 360 2996
Fragments arranged by size:
2996 1884 991 872 566 531 360 34
RsrII CG'GwC_CG
Cuts at: 4718 6429 4718
Size: 1711 6523
SacI G_AGCT'C
Cuts at: 2102 5248 2102
Size: 3146 5088
SalI G'TCGA_C
Cuts at: 5271 5271
Size: 8234
SapI GCTCTTCn'nnn_
Cuts at: 535 771 1845 2509 2939 3225 6257 6467 535
Size: 236 1074 664 430 286 3032 210 2302
Fragments arranged by size:
3032 2302 1074 664 430 286 236 210
SbfI CC_TGCA'GG
Cuts at: 3465 5281 3465
Size: 1816 6418
ScaI AGT'ACT
Cuts at: 7751 7751
Size: 8234
SexAI A'CCwGG_T
Cuts at: 1425 1425
Size: 8234
SfcI C'TryA_G
Cuts at: 33 1100 1766 3461 4168 4459 5277 5962 6930
Size: 1067 666 1695 707 291 818 685 968
Cuts at: 6930 7121 33
Size: 191 1146
Fragments arranged by size:
1695 1146 1067 968 818 707 685 666
291 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3883 3883
Size: 8234
SfoI GGC'GCC
Cuts at: 549 4269 4306 5666 5914 549
Size: 3720 37 1360 248 2869
Fragments arranged by size:
3720 2869 1360 248 37
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8234
SmaI CCC'GGG
Cuts at: 1795 3709 4567 5256 1795
Size: 1914 858 689 4773
Fragments arranged by size:
4773 1914 858 689
SmlI C'TyrA_G
Cuts at: 4795 5389 6304 7018 7280 4795
Size: 594 915 714 262 5749
Fragments arranged by size:
5749 915 714 594 262
SnaBI TAC'GTA
Cuts at: 4445 4445
Size: 8234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8234
SphI G_CATG'C
Cuts at: 5287 5787 5287
Size: 500 7734
StuI AGG'CCT
Cuts at: 210 2248 2347 2841 3459 210
Size: 2038 99 494 618 4985
Fragments arranged by size:
4985 2038 618 494 99
StyI C'CwwG_G
Cuts at: 107 188 551 720 912 1668 1805 2093 2642
Size: 81 363 169 192 756 137 288 549
Cuts at: 2642 3973 4859 5072 5368 5722 107
Size: 1331 886 213 296 354 2619
Fragments arranged by size:
2619 1331 886 756 549 363 354 296
288 213 192 169 137 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2028 2123 2439 3603 5817 5950 6409 6618 2028
Size: 95 316 1164 2214 133 459 209 3644
Fragments arranged by size:
3644 2214 1164 459 316 209 133 95
TatI w'GTAC_w
Cuts at: 7749 7749
Size: 8234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 565 597 1716 1748 2962 2994 7751 7783 565
Size: 32 1119 32 1214 32 4757 32 1016
Fragments arranged by size:
4757 1214 1119 1016 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6031 6031
Size: 8234
XbaI T'CTAG_A
Cuts at: 5265 5265
Size: 8234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5729 5729
Size: 8234
XmaI C'CCGG_G
Cuts at: 1793 3707 4565 5254 1793
Size: 1914 858 689 4773
Fragments arranged by size:
4773 1914 858 689
XmnI GAAnn'nnTTC
Cuts at: 1472 1472
Size: 8234
ZraI GAC'GTC
Cuts at: 2914 2914
Size: 8234
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI AscI BaeI BfrBI BmtI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SgrAI SrfI SspI SwaI XhoI