Restriction map for
pF1KA0336
(Circular) (Six-base) MAPSORT of: pF1KA0336.seq Check: 389 from: 1 to: 7862
pF1KA0336 7862 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1514 3101 4900 1514
Size: 1587 1799 4476
Fragments arranged by size:
4476 1799 1587
Acc65I G'GTAC_C
Cuts at: 1464 4878 1464
Size: 3414 4448
AclI AA'CG_TT
Cuts at: 3303 7702 3303
Size: 4399 3463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1856 1880 1981 2621 3112 3169 3202 3416
Size: 1503 24 101 640 491 57 33 214
Cuts at: 3416 4775 5687 6119 6472 7134 353
Size: 1359 912 432 353 662 1081
Fragments arranged by size:
1503 1359 1081 912 662 640 491 432
353 214 101 57 33 24
AflII C'TTAA_G
Cuts at: 886 1090 1189 2290 2331 4228 886
Size: 204 99 1101 41 1897 4520
Fragments arranged by size:
4520 1897 1101 204 99 41
AflIII A'CryG_T
Cuts at: 2467 5214 7014 7295 2467
Size: 2747 1800 281 3034
Fragments arranged by size:
3034 2747 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 4091 4091
Size: 7862
AlwNI CAG_nnn'CTG
Cuts at: 3523 5167 6605 3523
Size: 1644 1438 4780
Fragments arranged by size:
4780 1644 1438
ApaLI G'TGCA_C
Cuts at: 6700 6700
Size: 7862
ApoI r'AATT_y
Cuts at: 201 316 395 705 1268 1572 1617 2970 2997
Size: 115 79 310 563 304 45 1353 27
Cuts at: 2997 3377 3760 4468 4866 7215 7471 201
Size: 380 383 708 398 2349 256 592
Fragments arranged by size:
2349 1353 708 592 563 398 383 380
310 304 256 115 79 45 27
AseI AT'TA_AT
Cuts at: 20 506 965 1168 3926 20
Size: 486 459 203 2758 3956
Fragments arranged by size:
3956 2758 486 459 203
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7862
AvaI C'yCGr_G
Cuts at: 4337 4882 4337
Size: 545 7317
BamHI G'GATC_C
Cuts at: 4887 4887
Size: 7862
BanI G'GyrC_C
Cuts at: 1464 4878 5292 5540 5575 1464
Size: 3414 414 248 35 3751
Fragments arranged by size:
3751 3414 414 248 35
BanII G_rGCy'C
Cuts at: 4876 5906 4876
Size: 1030 6832
BbeI G_GCGC'C
Cuts at: 5296 5544 5296
Size: 248 7614
BbsI GAAGACnn'nnnn_
Cuts at: 251 953 1844 2126 2627 2954 3422 4376 251
Size: 702 891 282 501 327 468 954 3737
Fragments arranged by size:
3737 954 891 702 501 468 327 282
BciVI GTATCCnnnnn_n'
Cuts at: 5760 6816 7862 5760
Size: 1056 1046 5760
Fragments arranged by size:
5760 1056 1046
BclI T'GATC_A
Cuts at: 3525 5382 3525
Size: 1857 6005
BglI GCCn_nnn'nGGC
Cuts at: 2731 2731
Size: 7862
BglII A'GATC_T
Cuts at: 2835 3012 5377 7414 2835
Size: 177 2365 2037 3283
Fragments arranged by size:
3283 2365 2037 177
BlpI GC'TnA_GC
Cuts at: 4747 4974 4747
Size: 227 7635
Bme1580I G_kGCm'C
Cuts at: 5487 5580 6704 5487
Size: 93 1124 6645
Fragments arranged by size:
6645 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1294 4249 5074 5155 5490 7634 1294
Size: 2955 825 81 335 2144 1522
Fragments arranged by size:
2955 2144 1522 825 335 81
BmtI G_CTAG'C
Cuts at: 1030 1030
Size: 7862
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6229 6229
Size: 7862
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 3583 1408
Size: 2175 5687
Bpu10I CC'TnA_GC
Cuts at: 593 2598 593
Size: 2005 5857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4465 4769 5038 5917 6631 6929 4465
Size: 304 269 879 714 298 5398
Fragments arranged by size:
5398 879 714 304 298 269
BsaI GGTCTCn'nnnn_
Cuts at: 2758 7566 2758
Size: 4808 3054
BsaAI yAC'GTr
Cuts at: 5215 5215
Size: 7862
BsaBI GATnn'nnATC
Cuts at: 6 4824 5376 7413 6
Size: 4818 552 2037 455
Fragments arranged by size:
4818 2037 552 455
BsaHI Gr'CG_yC
Cuts at: 5293 5541 6243 7739 5293
Size: 248 702 1496 5416
Fragments arranged by size:
5416 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5051 5572 6250 6661 6808 7082 5051
Size: 521 678 411 147 274 5831
Fragments arranged by size:
5831 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4366 4396 4366
Size: 30 7832
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 1777 2710 403
Size: 1374 933 5555
Fragments arranged by size:
5555 1374 933
BseYI C'CCAG_C
Cuts at: 5287 6045 6710 7118 5287
Size: 758 665 408 6031
Fragments arranged by size:
6031 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4538 4538
Size: 7862
BsiEI CG_ry'CG
Cuts at: 104 5404 5450 6680 104
Size: 5300 46 1230 1286
Fragments arranged by size:
5300 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3644 4876 5654 5844 6704 3644
Size: 1232 778 190 860 4802
Fragments arranged by size:
4802 1232 860 778 190
BsiWI C'GTAC_G
Cuts at: 3673 3673
Size: 7862
BsmI GAATG_Cn'
Cuts at: 2406 2686 2406
Size: 280 7582
Bsp1286I G_dGCh'C
Cuts at: 3644 4876 5487 5580 5654 5844 5906 6704 3644
Size: 1232 611 93 74 190 62 798 4802
Fragments arranged by size:
4802 1232 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 936 1353 6294 936
Size: 417 4941 2504
Fragments arranged by size:
4941 2504 417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1021 4912 5126 5428 5809 1021
Size: 3891 214 302 381 3074
Fragments arranged by size:
3891 3074 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6154 6208 7692 7857 50
Size: 6104 54 1484 165 55
Fragments arranged by size:
6104 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 287 3101 3946 5774 287
Size: 2814 845 1828 2375
Fragments arranged by size:
2814 2375 1828 845
BsrFI r'CCGG_y
Cuts at: 5860 6041 5860
Size: 181 7681
BssSI C'ACGA_G
Cuts at: 3637 6133 6841 3637
Size: 2496 708 4658
Fragments arranged by size:
4658 2496 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4561 5239 4561
Size: 678 7184
BstBI TT'CG_AA
Cuts at: 1319 4122 6223 1319
Size: 2803 2101 2958
Fragments arranged by size:
2958 2803 2101
BstXI CCAn_nnnn'nTGG
Cuts at: 3096 3096
Size: 7862
BstYI r'GATC_y
Cuts at: 1 2835 3012 4887 5354 5377 5712 5958 6264
Size: 2834 177 1875 467 23 335 246 306
Cuts at: 6264 6276 6362 7414 1
Size: 12 86 1052 449
Fragments arranged by size:
2834 1875 1052 467 449 335 306 246
177 86 23 12
BstZ17I GTA'TAC
Cuts at: 1515 1515
Size: 7862
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5812 5993 6156 5812
Size: 181 163 7518
Fragments arranged by size:
7518 181 163
BtsI GCAGTG_nn'
Cuts at: 2989 3967 5105 2989
Size: 978 1138 5746
Fragments arranged by size:
5746 1138 978
DraI TTT'AAA
Cuts at: 960 1037 1238 1772 2027 3551 4861 960
Size: 77 201 534 255 1524 1310 3961
Fragments arranged by size:
3961 1524 1310 534 255 201 77
DrdI GACnn_nn'nnGTC
Cuts at: 1137 3039 5568 6912 1137
Size: 1902 2529 1344 2087
Fragments arranged by size:
2529 2087 1902 1344
EaeI y'GGCC_r
Cuts at: 5447 5621 6012 6039 7384 5447
Size: 174 391 27 1345 5925
Fragments arranged by size:
5925 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5447 5447
Size: 7862
EarI CTCTTCn'nnn_
Cuts at: 353 515 548 2006 2123 2258 2801 3710 4225
Size: 162 33 1458 117 135 543 909 515
Cuts at: 4225 4337 5885 6095 353
Size: 112 1548 210 2120
Fragments arranged by size:
2120 1548 1458 909 543 515 210 162
135 117 112 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4033 4377 6070 6812 6958 7672 4033
Size: 344 1693 742 146 714 4223
Fragments arranged by size:
4223 1693 742 714 344 146
EcoICRI GAG'CTC
Cuts at: 4874 4874
Size: 7862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1408 1856 1880 1981 2621 3112 3169 3202
Size: 1055 448 24 101 640 491 57 33
Cuts at: 3202 3416 3583 4775 5687 6119 6472 7134 353
Size: 214 167 1192 912 432 353 662 1081
Fragments arranged by size:
1192 1081 1055 912 662 640 491 448
432 353 214 167 101 57 33 24
EcoO109I rG'GnC_Cy
Cuts at: 2370 4116 5001 2370
Size: 1746 885 5231
Fragments arranged by size:
5231 1746 885
EcoRI G'AATT_C
Cuts at: 3760 4866 3760
Size: 1106 6756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1417 2613 4686 5881 1417
Size: 1196 2073 1195 3398
Fragments arranged by size:
3398 2073 1196 1195
FspI TGC'GCA
Cuts at: 5643 5643
Size: 7862
HaeII r_GCGC'y
Cuts at: 3845 5296 5544 6774 7545 3845
Size: 1451 248 1230 771 4162
Fragments arranged by size:
4162 1451 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1306 1338 1560 1592 2617 2649 2803 2835 4077
Size: 32 222 32 1025 32 154 32 1242
Cuts at: 4077 4109 4360 4366 4392 4398 5704 5734 5736
Size: 32 251 6 26 6 1306 30 2
Cuts at: 5736 5766 1306
Size: 30 3402
Fragments arranged by size:
3402 1306 1242 1025 251 222 154 32
32 32 32 32 30 30 26 6
6 2
HincII GTy'rAC
Cuts at: 3332 4901 3332
Size: 1569 6293
HindIII A'AGCT_T
Cuts at: 4585 4585
Size: 7862
HpaI GTT'AAC
Cuts at: 3332 3332
Size: 7862
KasI G'GCGC_C
Cuts at: 5292 5540 5292
Size: 248 7614
KpnI G_GTAC'C
Cuts at: 1468 4882 1468
Size: 3414 4448
MfeI C'AATT_G
Cuts at: 3472 3472
Size: 7862
MluI A'CGCG_T
Cuts at: 7295 7295
Size: 7862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 2630 6621 6805 143
Size: 2487 3991 184 1200
Fragments arranged by size:
3991 2487 1200 184
MscI TGG'CCA
Cuts at: 5623 5623
Size: 7862
MslI CAynn'nnrTG
Cuts at: 3274 4063 5851 5978 3274
Size: 789 1788 127 5158
Fragments arranged by size:
5158 1788 789 127
MspA1I CmG'CkG
Cuts at: 270 1858 3603 4419 4973 5287 5647 6431 6676
Size: 1588 1745 816 554 314 360 784 245
Cuts at: 6676 270
Size: 1456
Fragments arranged by size:
1745 1588 1456 816 784 554 360 314
245
NaeI GCC'GGC
Cuts at: 6043 6043
Size: 7862
NarI GG'CG_CC
Cuts at: 5293 5541 5293
Size: 248 7614
NgoMIV G'CCGG_C
Cuts at: 6041 6041
Size: 7862
NheI G'CTAG_C
Cuts at: 1026 1026
Size: 7862
NspI r_CATG'y
Cuts at: 2471 3157 4326 4465 4915 5415 7018 2471
Size: 686 1169 139 450 500 1603 3315
Fragments arranged by size:
3315 1603 1169 686 500 450 139
PciI A'CATG_T
Cuts at: 2467 7014 2467
Size: 4547 3315
PfoI T'CCnGG_A
Cuts at: 4025 4025
Size: 7862
PmeI GTTT'AAAC
Cuts at: 4861 4861
Size: 7862
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6308 6340 6308
Size: 32 7830
PpuMI rG'GwC_Cy
Cuts at: 4116 4116
Size: 7862
PsiI TTA'TAA
Cuts at: 1603 2512 4729 1603
Size: 909 2217 4736
Fragments arranged by size:
4736 2217 909
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1368 1400 3173 3205 1368
Size: 32 1773 32 6025
Fragments arranged by size:
6025 1773 32 32
PstI C_TGCA'G
Cuts at: 1364 2378 3998 4644 4679 4909 5594 1364
Size: 1014 1620 646 35 230 685 3632
Fragments arranged by size:
3632 1620 1014 685 646 230 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7862
PvuII CAG'CTG
Cuts at: 270 1858 3603 4419 5287 5647 270
Size: 1588 1745 816 868 360 2485
Fragments arranged by size:
2485 1745 1588 868 816 360
RsrII CG'GwC_CG
Cuts at: 6057 6057
Size: 7862
SacI G_AGCT'C
Cuts at: 4876 4876
Size: 7862
SalI G'TCGA_C
Cuts at: 4899 4899
Size: 7862
SapI GCTCTTCn'nnn_
Cuts at: 515 2006 4225 5885 6095 515
Size: 1491 2219 1660 210 2282
Fragments arranged by size:
2282 2219 1660 1491 210
SbfI CC_TGCA'GG
Cuts at: 4909 4909
Size: 7862
ScaI AGT'ACT
Cuts at: 7379 7379
Size: 7862
SexAI A'CCwGG_T
Cuts at: 4695 4695
Size: 7862
SfcI C'TryA_G
Cuts at: 33 1058 1210 1360 2152 2374 3709 3994 4640
Size: 1025 152 150 792 222 1335 285 646
Cuts at: 4640 4675 4905 5590 6558 6749 33
Size: 35 230 685 968 191 1146
Fragments arranged by size:
1335 1146 1025 968 792 685 646 285
230 222 191 152 150 35
SfoI GGC'GCC
Cuts at: 5294 5542 5294
Size: 248 7614
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7862
SmaI CCC'GGG
Cuts at: 4339 4884 4339
Size: 545 7317
SmlI C'TyrA_G
Cuts at: 886 1090 1189 2290 2331 4228 4444 4784 5017
Size: 204 99 1101 41 1897 216 340 233
Cuts at: 5017 5932 6646 6908 886
Size: 915 714 262 1840
Fragments arranged by size:
1897 1840 1101 915 714 340 262 233
216 204 99 41
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7862
SphI G_CATG'C
Cuts at: 4465 4915 5415 4465
Size: 450 500 6912
Fragments arranged by size:
6912 500 450
SspI AAT'ATT
Cuts at: 504 504
Size: 7862
StyI C'CwwG_G
Cuts at: 2171 3731 4996 5350 2171
Size: 1560 1265 354 4683
Fragments arranged by size:
4683 1560 1265 354
SwaI ATTT'AAAT
Cuts at: 2027 2027
Size: 7862
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5445 5578 6037 6246 5445
Size: 133 459 209 7061
Fragments arranged by size:
7061 459 209 133
TatI w'GTAC_w
Cuts at: 1080 1712 3063 3435 7377 1080
Size: 632 1351 372 3942 1565
Fragments arranged by size:
3942 1565 1351 632 372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4021 4053 4205 4237 7379 7411 4021
Size: 32 152 32 3142 32 4472
Fragments arranged by size:
4472 3142 152 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5659 5659
Size: 7862
XbaI T'CTAG_A
Cuts at: 2388 4317 4893 2388
Size: 1929 576 5357
Fragments arranged by size:
5357 1929 576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3495 4482 5357 3495
Size: 987 875 6000
Fragments arranged by size:
6000 987 875
XmaI C'CCGG_G
Cuts at: 4337 4882 4337
Size: 545 7317
XmnI GAAnn'nnTTC
Cuts at: 2795 3125 3182 4487 2795
Size: 330 57 1305 6170
Fragments arranged by size:
6170 1305 330 57
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PciI
PfoI PmeI PpiI PpuMI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BsmBI
BspEI BsrGI BssHII BstEII Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI NcoI NdeI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PspOMI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
XhoI ZraI