Restriction map for
pF1KA0329
(Circular) (Six-base) MAPSORT of: pF1KA0329.seq Check: 5881 from: 1 to: 7346
pF1KA0329 7346 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2655 3956 2655
Size: 1301 6045
AatII G_ACGT'C
Cuts at: 2359 2359
Size: 7346
AccI GT'mk_AC
Cuts at: 4384 4384
Size: 7346
Acc65I G'GTAC_C
Cuts at: 4362 4362
Size: 7346
AclI AA'CG_TT
Cuts at: 1744 7186 1744
Size: 5442 1904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 1193 3313 5171 5603 6350 6618 722
Size: 471 2120 1858 432 747 268 1450
Fragments arranged by size:
2120 1858 1450 747 471 432 268
AflIII A'CryG_T
Cuts at: 2253 4698 5802 6779 2253
Size: 2445 1104 977 2820
Fragments arranged by size:
2820 2445 1104 977
AgeI A'CCGG_T
Cuts at: 5734 5734
Size: 7346
AhdI GACnn_n'nnGTC
Cuts at: 1620 1620
Size: 7346
AleI CACnn'nnGTG
Cuts at: 349 349
Size: 7346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 289 2632 289
Size: 2343 5003
AlwNI CAG_nnn'CTG
Cuts at: 127 400 1273 1425 2097 2454 3002 3463 3948
Size: 273 873 152 672 357 548 461 485
Cuts at: 3948 4192 4651 6218 127
Size: 244 459 1567 1255
Fragments arranged by size:
1567 1255 873 672 548 485 461 459
357 273 244 152
ApaI G_GGCC'C
Cuts at: 2079 2973 3102 4086 4222 2079
Size: 894 129 984 136 5203
Fragments arranged by size:
5203 984 894 136 129
ApaLI G'TGCA_C
Cuts at: 1107 3976 6116 1107
Size: 2869 2140 2337
Fragments arranged by size:
2869 2337 2140
ApoI r'AATT_y
Cuts at: 754 1157 1579 3394 4160 4350 6699 6955 754
Size: 403 422 1815 766 190 2349 256 1145
Fragments arranged by size:
2349 1815 1145 766 422 403 256 190
AseI AT'TA_AT
Cuts at: 20 20
Size: 7346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7346
AvaI C'yCGr_G
Cuts at: 820 2526 3076 3349 3381 4366 820
Size: 1706 550 273 32 985 3800
Fragments arranged by size:
3800 1706 985 550 273 32
BamHI G'GATC_C
Cuts at: 1081 4371 1081
Size: 3290 4056
BanI G'GyrC_C
Cuts at: 293 968 2579 2623 3424 4273 4362 4776 5024
Size: 675 1611 44 801 849 89 414 248
Cuts at: 5024 5059 293
Size: 35 2580
Fragments arranged by size:
2580 1611 849 801 675 414 248 89
44 35
BanII G_rGCy'C
Cuts at: 517 593 827 1373 1388 2079 2138 2429 2691
Size: 76 234 546 15 691 59 291 262
Cuts at: 2691 2973 3102 4086 4222 4360 5390 517
Size: 282 129 984 136 138 1030 2473
Fragments arranged by size:
2473 1030 984 691 546 291 282 262
234 138 136 129 76 59 15
BbeI G_GCGC'C
Cuts at: 2627 4780 5028 2627
Size: 2153 248 4945
Fragments arranged by size:
4945 2153 248
BbsI GAAGACnn'nnnn_
Cuts at: 337 1525 1997 337
Size: 1188 472 5686
Fragments arranged by size:
5686 1188 472
BbvCI CC'TCA_GC
Cuts at: 1790 2068 2185 2548 1790
Size: 278 117 363 6588
Fragments arranged by size:
6588 363 278 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 249 283 3057 3091 249
Size: 34 2774 34 4504
Fragments arranged by size:
4504 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5244 6005 7346 5244
Size: 761 1341 5244
Fragments arranged by size:
5244 1341 761
BclI T'GATC_A
Cuts at: 4866 4866
Size: 7346
BglI GCCn_nnn'nGGC
Cuts at: 1383 3710 4226 1383
Size: 2327 516 4503
Fragments arranged by size:
4503 2327 516
BglII A'GATC_T
Cuts at: 4861 6898 4861
Size: 2037 5309
BlpI GC'TnA_GC
Cuts at: 361 4063 4458 361
Size: 3702 395 3249
Fragments arranged by size:
3702 3249 395
Bme1580I G_kGCm'C
Cuts at: 1111 2079 2973 3102 3980 4086 4222 4971 5064
Size: 968 894 129 878 106 136 749 93
Cuts at: 5064 6120 1111
Size: 1056 2337
Fragments arranged by size:
2337 1056 968 894 878 749 136 129
106 93
BmgBI CAC'GTC
Cuts at: 3402 3402
Size: 7346
BmrI ACTGGGnnnn_n'
Cuts at: 758 1593 3845 4558 4639 4974 7118 758
Size: 835 2252 713 81 335 2144 986
Fragments arranged by size:
2252 2144 986 835 713 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 5713 2710
Size: 3003 4343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 1225 1372 1661 1777 4339 560
Size: 665 147 289 116 2562 3567
Fragments arranged by size:
3567 2562 665 289 147 116
Bpu10I CC'TnA_GC
Cuts at: 1400 1790 2068 2185 2455 2548 2803 3407 1400
Size: 390 278 117 270 93 255 604 5339
Fragments arranged by size:
5339 604 390 278 270 255 117 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1617 2347 2473 4522 5401 5893 6191 1617
Size: 730 126 2049 879 492 298 2772
Fragments arranged by size:
2772 2049 879 730 492 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 672 2295 2565 4094 7050 672
Size: 1623 270 1529 2956 968
Fragments arranged by size:
2956 1623 1529 968 270
BsaAI yAC'GTr
Cuts at: 1236 3981 4699 1236
Size: 2745 718 3883
Fragments arranged by size:
3883 2745 718
BsaBI GATnn'nnATC
Cuts at: 6 1182 4860 6897 6
Size: 1176 3678 2037 455
Fragments arranged by size:
3678 2037 1176 455
BsaHI Gr'CG_yC
Cuts at: 2356 2477 2624 4121 4777 5025 5727 7223 2356
Size: 121 147 1497 656 248 702 1496 2479
Fragments arranged by size:
2479 1497 1496 702 656 248 147 121
BsaWI w'CCGG_w
Cuts at: 1084 2286 3006 4535 5056 5734 6008 6155 6566
Size: 1202 720 1529 521 678 274 147 411
Cuts at: 6566 1084
Size: 1864
Fragments arranged by size:
1864 1529 1202 720 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1348 1378 1530 1560 4312 4342 1348
Size: 30 152 30 2752 30 4352
Fragments arranged by size:
4352 2752 152 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 631 2383 3369 4015 4225 154
Size: 477 1752 986 646 210 3275
Fragments arranged by size:
3275 1752 986 646 477 210
BseYI C'CCAG_C
Cuts at: 602 1004 1404 2072 2137 2444 2463 3320 3740
Size: 402 400 668 65 307 19 857 420
Cuts at: 3740 4771 5529 6106 6602 602
Size: 1031 758 577 496 1346
Fragments arranged by size:
1346 1031 857 758 668 577 496 420
402 400 307 65 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 901 1258 1688 2489 3237 3573 3616 655
Size: 246 357 430 801 748 336 43 4385
Fragments arranged by size:
4385 801 748 430 357 336 246 43
BsiEI CG_ry'CG
Cuts at: 104 4888 4934 6142 104
Size: 4784 46 1208 1308
Fragments arranged by size:
4784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1111 1543 2429 3980 4069 4360 5138 5328 6120
Size: 432 886 1551 89 291 778 190 792
Cuts at: 6120 1111
Size: 2337
Fragments arranged by size:
2337 1551 886 792 778 432 291 190
89
BsmBI CGTCTCn'nnnn_
Cuts at: 1270 2404 1270
Size: 1134 6212
Bsp1286I G_dGCh'C
Cuts at: 517 593 827 1111 1373 1388 1543 2079 2138
Size: 76 234 284 262 15 155 536 59
Cuts at: 2138 2429 2691 2973 3102 3980 4069 4086 4222
Size: 291 262 282 129 878 89 17 136
Cuts at: 4222 4360 4971 5064 5138 5328 5390 6120 517
Size: 138 611 93 74 190 62 730 1743
Fragments arranged by size:
1743 878 730 611 536 291 284 282
262 262 234 190 155 138 136 129
93 89 76 74 62 59 17 15
BspEI T'CCGG_A
Cuts at: 3006 6566 3006
Size: 3560 3786
BspHI T'CATG_A
Cuts at: 6522 6522
Size: 7346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2655 3956 4396 4610 4912 5293 2655
Size: 1301 440 214 302 381 4708
Fragments arranged by size:
4708 1301 440 381 302 214
BsrBI CCG'CTC
Cuts at: 50 203 5638 5692 7176 7341 50
Size: 153 5435 54 1484 165 55
Fragments arranged by size:
5435 1484 165 153 55 54
BsrDI GCAATG_nn'
Cuts at: 433 5258 433
Size: 4825 2521
BsrFI r'CCGG_y
Cuts at: 290 2459 5344 5525 5734 290
Size: 2169 2885 181 209 1902
Fragments arranged by size:
2885 2169 1902 209 181
BssHII G'CGCG_C
Cuts at: 3621 3621
Size: 7346
BssSI C'ACGA_G
Cuts at: 5617 5975 5617
Size: 358 6988
BstAPI GCAn_nnn'nTGC
Cuts at: 4723 4723
Size: 7346
BstBI TT'CG_AA
Cuts at: 5707 5707
Size: 7346
BstXI CCAn_nnnn'nTGG
Cuts at: 1311 1837 2005 1311
Size: 526 168 6652
Fragments arranged by size:
6652 526 168
BstYI r'GATC_y
Cuts at: 1 185 576 1081 3374 4371 4838 4861 5196
Size: 184 391 505 2293 997 467 23 335
Cuts at: 5196 5442 6454 6540 6552 6898 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2293 1012 997 505 467 449 391 346
335 246 184 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2682 4310 2682
Size: 1628 5718
BtgI C'CryG_G
Cuts at: 107 849 1298 1310 2129 2338 2385 3111 3450
Size: 742 449 12 819 209 47 726 339
Cuts at: 3450 3590 3707 107
Size: 140 117 3746
Fragments arranged by size:
3746 819 742 726 449 339 209 140
117 47 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 234 2378 2822 5296 5477 5640 234
Size: 2144 444 2474 181 163 1940
Fragments arranged by size:
2474 2144 1940 444 181 163
BtsI GCAGTG_nn'
Cuts at: 1887 2795 3025 4589 1887
Size: 908 230 1564 4644
Fragments arranged by size:
4644 1564 908 230
ClaI AT'CG_AT
Cuts at: 115 115
Size: 7346
DraI TTT'AAA
Cuts at: 4345 4345
Size: 7346
DraIII CAC_nnn'GTG
Cuts at: 3255 3255
Size: 7346
DrdI GACnn_nn'nnGTC
Cuts at: 2355 5052 5910 2355
Size: 2697 858 3791
Fragments arranged by size:
3791 2697 858
EaeI y'GGCC_r
Cuts at: 1301 1313 2178 3257 3669 4931 5105 5496 5523
Size: 12 865 1079 412 1262 174 391 27
Cuts at: 5523 6868 1301
Size: 1345 1779
Fragments arranged by size:
1779 1345 1262 1079 865 412 391 174
27 12
EagI C'GGCC_G
Cuts at: 4931 4931
Size: 7346
EarI CTCTTCn'nnn_
Cuts at: 1299 4276 5369 5579 1299
Size: 2977 1093 210 3066
Fragments arranged by size:
3066 2977 1093 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5554 5864 6010 7156 5554
Size: 310 146 1146 5744
Fragments arranged by size:
5744 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2427 4358 2427
Size: 1931 5415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 722 1193 1225 1372 1661 1777 3313 4339
Size: 162 471 32 147 289 116 1536 1026
Cuts at: 4339 5171 5603 6350 6618 560
Size: 832 432 747 268 1288
Fragments arranged by size:
1536 1288 1026 832 747 471 432 289
268 162 147 116 32
EcoNI CCTnn'n_nnAGG
Cuts at: 402 2782 3670 402
Size: 2380 888 4078
Fragments arranged by size:
4078 2380 888
EcoO109I rG'GnC_Cy
Cuts at: 2076 2451 2530 2841 2969 2970 3099 3546 4314
Size: 375 79 311 128 1 129 447 768
Cuts at: 4314 4485 2076
Size: 171 4937
Fragments arranged by size:
4937 768 447 375 311 171 129 128
79 1
EcoRI G'AATT_C
Cuts at: 3394 4350 3394
Size: 956 6390
EcoRV GAT'ATC
Cuts at: 119 119
Size: 7346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 3068 5365 336
Size: 2732 2297 2317
Fragments arranged by size:
2732 2317 2297
FspI TGC'GCA
Cuts at: 3304 5127 3304
Size: 1823 5523
HaeII r_GCGC'y
Cuts at: 2627 2956 3566 3658 4063 4780 5028 6050 7029
Size: 329 610 92 405 717 248 1022 979
Cuts at: 7029 2627
Size: 2944
Fragments arranged by size:
2944 1022 979 717 610 405 329 248
92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 455 487 607 639 1081 1113 1185 1217 1348
Size: 32 120 32 442 32 72 32 131
Cuts at: 1348 1380 1530 1562 1606 1638 2856 2888 3825
Size: 32 150 32 44 32 1218 32 937
Cuts at: 3825 3857 5188 5218 5220 5250 455
Size: 32 1331 30 2 30 2551
Fragments arranged by size:
2551 1331 1218 937 442 150 131 120
72 44 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1053 4385 1053
Size: 3332 4014
HindIII A'AGCT_T
Cuts at: 3050 3516 3765 3050
Size: 466 249 6631
Fragments arranged by size:
6631 466 249
KasI G'GCGC_C
Cuts at: 2623 4776 5024 2623
Size: 2153 248 4945
Fragments arranged by size:
4945 2153 248
KpnI G_GTAC'C
Cuts at: 4366 4366
Size: 7346
MfeI C'AATT_G
Cuts at: 1064 1064
Size: 7346
MluI A'CGCG_T
Cuts at: 6779 6779
Size: 7346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 202 593 997 2022 4263 6017 6201 202
Size: 391 404 1025 2241 1754 184 1347
Fragments arranged by size:
2241 1754 1347 1025 404 391 184
MscI TGG'CCA
Cuts at: 1303 1315 2180 3259 5107 1303
Size: 12 865 1079 1848 3542
Fragments arranged by size:
3542 1848 1079 865 12
MslI CAynn'nnrTG
Cuts at: 253 271 349 1835 3712 5335 5462 253
Size: 18 78 1486 1877 1623 127 2137
Fragments arranged by size:
2137 1877 1623 1486 127 78 18
MspA1I CmG'CkG
Cuts at: 606 639 1361 2072 2280 2916 3365 3600 4056
Size: 33 722 711 208 636 449 235 456
Cuts at: 4056 4088 4457 4771 5131 6144 6389 606
Size: 32 369 314 360 1013 245 1563
Fragments arranged by size:
1563 1013 722 711 636 456 449 369
360 314 245 235 208 33 32
NaeI GCC'GGC
Cuts at: 292 2461 5527 292
Size: 2169 3066 2111
Fragments arranged by size:
3066 2169 2111
NarI GG'CG_CC
Cuts at: 2624 4777 5025 2624
Size: 2153 248 4945
Fragments arranged by size:
4945 2153 248
NcoI C'CATG_G
Cuts at: 107 1298 3111 107
Size: 1191 1813 4342
Fragments arranged by size:
4342 1813 1191
NgoMIV G'CCGG_C
Cuts at: 290 2459 5525 290
Size: 2169 3066 2111
Fragments arranged by size:
3066 2169 2111
NspI r_CATG'y
Cuts at: 276 2206 3717 3776 4033 4399 4899 5806 276
Size: 1930 1511 59 257 366 500 907 1816
Fragments arranged by size:
1930 1816 1511 907 500 366 257 59
PasI CC'CwG_GG
Cuts at: 1381 1381
Size: 7346
PciI A'CATG_T
Cuts at: 5802 5802
Size: 7346
PflMI CCAn_nnn'nTGG
Cuts at: 2667 2667
Size: 7346
PfoI T'CCnGG_A
Cuts at: 1754 1754
Size: 7346
PmeI GTTT'AAAC
Cuts at: 4345 4345
Size: 7346
PmlI CAC'GTG
Cuts at: 1236 3981 1236
Size: 2745 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6485 6517 6485
Size: 32 7314
PpuMI rG'GwC_Cy
Cuts at: 2451 2530 2841 3546 4314 2451
Size: 79 311 705 768 5483
Fragments arranged by size:
5483 768 705 311 79
PshAI GACnn'nnGTC
Cuts at: 2225 2287 2225
Size: 62 7284
PsiI TTA'TAA
Cuts at: 1153 1153
Size: 7346
PspOMI G'GGCC_C
Cuts at: 2075 2969 3098 4082 4218 2075
Size: 894 129 984 136 5203
Fragments arranged by size:
5203 984 894 136 129
PstI C_TGCA'G
Cuts at: 1920 2611 3498 4393 5078 1920
Size: 691 887 895 685 4188
Fragments arranged by size:
4188 895 887 691 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7346
PvuII CAG'CTG
Cuts at: 606 639 2072 2280 3600 4056 4771 5131 606
Size: 33 1433 208 1320 456 715 360 2821
Fragments arranged by size:
2821 1433 1320 715 456 360 208 33
RsrII CG'GwC_CG
Cuts at: 3986 5541 3986
Size: 1555 5791
SacI G_AGCT'C
Cuts at: 2429 4360 2429
Size: 1931 5415
SalI G'TCGA_C
Cuts at: 4383 4383
Size: 7346
SanDI GG'GwC_CC
Cuts at: 2530 2530
Size: 7346
SapI GCTCTTCn'nnn_
Cuts at: 1299 4276 5369 5579 1299
Size: 2977 1093 210 3066
Fragments arranged by size:
3066 2977 1093 210
SbfI CC_TGCA'GG
Cuts at: 4393 4393
Size: 7346
ScaI AGT'ACT
Cuts at: 747 6863 747
Size: 6116 1230
SexAI A'CCwGG_T
Cuts at: 377 377
Size: 7346
SfcI C'TryA_G
Cuts at: 33 1916 2607 3494 4389 5074 6067 6258 33
Size: 1883 691 887 895 685 993 191 1121
Fragments arranged by size:
1883 1121 993 895 887 691 685 191
SfoI GGC'GCC
Cuts at: 2625 4778 5026 2625
Size: 2153 248 4945
Fragments arranged by size:
4945 2153 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7346
SmaI CCC'GGG
Cuts at: 822 4368 822
Size: 3546 3800
SmlI C'TyrA_G
Cuts at: 1632 2326 2488 4501 5416 5908 6170 1632
Size: 694 162 2013 915 492 262 2808
Fragments arranged by size:
2808 2013 915 694 492 262 162
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7346
SphI G_CATG'C
Cuts at: 276 2206 3717 4399 4899 276
Size: 1930 1511 682 500 2723
Fragments arranged by size:
2723 1930 1511 682 500
SrfI GCCC'GGGC
Cuts at: 822 822
Size: 7346
SspI AAT'ATT
Cuts at: 1124 1124
Size: 7346
StuI AGG'CCT
Cuts at: 1170 2173 4048 1170
Size: 1003 1875 4468
Fragments arranged by size:
4468 1875 1003
StyI C'CwwG_G
Cuts at: 107 736 1298 1324 2297 3111 3156 4480 4834
Size: 629 562 26 973 814 45 1324 354
Cuts at: 4834 107
Size: 2619
Fragments arranged by size:
2619 1324 973 814 629 562 354 45
26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 996 2455 3942 4291 4929 5062 5521 5730 996
Size: 1459 1487 349 638 133 459 209 2612
Fragments arranged by size:
2612 1487 1459 638 459 349 209 133
TatI w'GTAC_w
Cuts at: 155 284 314 745 6861 155
Size: 129 30 431 6116 640
Fragments arranged by size:
6116 640 431 129 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 744 776 3634 3666 4312 4344 6863 6895 744
Size: 32 2858 32 646 32 2519 32 1195
Fragments arranged by size:
2858 2519 1195 646 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1048 3034 4194 5143 1048
Size: 1986 1160 949 3251
Fragments arranged by size:
3251 1986 1160 949
XbaI T'CTAG_A
Cuts at: 579 4377 579
Size: 3798 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 636 1401 3252 4841 636
Size: 765 1851 1589 3141
Fragments arranged by size:
3141 1851 1589 765
XmaI C'CCGG_G
Cuts at: 820 4366 820
Size: 3546 3800
XmnI GAAnn'nnTTC
Cuts at: 196 532 3394 196
Size: 336 2862 4148
Fragments arranged by size:
4148 2862 336
ZraI GAC'GTC
Cuts at: 2357 2357
Size: 7346
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsrGI BstEII BstZ17I CspCI FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PspXI
PsrI SacII SfiI SgrAI SnaBI SwaI XhoI