Restriction map for
pF1KA0324
(Circular) (Six-base) MAPSORT of: pF1KA0324.seq Check: 8131 from: 1 to: 11369
pF1KA0324 11369 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1206 1881 1974 5589 6858 7644 1206
Size: 675 93 3615 1269 786 4931
Fragments arranged by size:
4931 3615 1269 786 675 93
AatII G_ACGT'C
Cuts at: 2935 5172 5469 7890 2935
Size: 2237 297 2421 6414
Fragments arranged by size:
6414 2421 2237 297
AccI GT'mk_AC
Cuts at: 8407 8407
Size: 11369
Acc65I G'GTAC_C
Cuts at: 8385 8385
Size: 11369
AclI AA'CG_TT
Cuts at: 6228 11209 6228
Size: 4981 6388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 2273 3863 3944 4934 5075 5804 6441 7855
Size: 1284 1590 81 990 141 729 637 1414
Cuts at: 7855 9194 9626 10373 10641 989
Size: 1339 432 747 268 1717
Fragments arranged by size:
1717 1590 1414 1339 1284 990 747 729
637 432 268 141 81
AflII C'TTAA_G
Cuts at: 1263 1263
Size: 11369
AflIII A'CryG_T
Cuts at: 8721 9825 10802 8721
Size: 1104 977 9288
Fragments arranged by size:
9288 1104 977
AgeI A'CCGG_T
Cuts at: 9757 9757
Size: 11369
AhdI GACnn_n'nnGTC
Cuts at: 8172 8172
Size: 11369
AleI CACnn'nnGTG
Cuts at: 1668 1668
Size: 11369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3803 7679 3803
Size: 3876 7493
AlwNI CAG_nnn'CTG
Cuts at: 601 772 3424 6814 7048 7111 7153 8674 10241
Size: 171 2652 3390 234 63 42 1521 1567
Cuts at: 10241 601
Size: 1729
Fragments arranged by size:
3390 2652 1729 1567 1521 234 171 63
42
ApaI G_GGCC'C
Cuts at: 888 5001 7653 888
Size: 4113 2652 4604
Fragments arranged by size:
4604 4113 2652
ApaLI G'TGCA_C
Cuts at: 10139 10139
Size: 11369
ApoI r'AATT_y
Cuts at: 365 3558 3664 4102 8373 10722 10978 365
Size: 3193 106 438 4271 2349 256 756
Fragments arranged by size:
4271 3193 2349 756 438 256 106
AseI AT'TA_AT
Cuts at: 20 20
Size: 11369
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11369
AvaI C'yCGr_G
Cuts at: 137 319 578 1307 1333 4350 4470 4994 5705
Size: 182 259 729 26 3017 120 524 711
Cuts at: 5705 6293 8389 137
Size: 588 2096 3117
Fragments arranged by size:
3117 3017 2096 729 711 588 524 259
182 120 26
AvrII C'CTAG_G
Cuts at: 4585 4585
Size: 11369
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3026 3059 3026
Size: 33 11336
BamHI G'GATC_C
Cuts at: 2506 3199 4726 8394 2506
Size: 693 1527 3668 5481
Fragments arranged by size:
5481 3668 1527 693
BanI G'GyrC_C
Cuts at: 148 236 5276 6188 6679 6750 6939 7260 7608
Size: 88 5040 912 491 71 189 321 348
Cuts at: 7608 8385 8799 9047 9082 148
Size: 777 414 248 35 2435
Fragments arranged by size:
5040 2435 912 777 491 414 348 321
248 189 88 71 35
BanII G_rGCy'C
Cuts at: 888 2833 4622 5001 5141 7380 7653 7804 8383
Size: 1945 1789 379 140 2239 273 151 579
Cuts at: 8383 9413 888
Size: 1030 2844
Fragments arranged by size:
2844 2239 1945 1789 1030 579 379 273
151 140
BbeI G_GCGC'C
Cuts at: 240 6192 8803 9051 240
Size: 5952 2611 248 2558
Fragments arranged by size:
5952 2611 2558 248
BbsI GAAGACnn'nnnn_
Cuts at: 1121 1343 2444 3401 3692 3869 4289 4427 5291
Size: 222 1101 957 291 177 420 138 864
Cuts at: 5291 5321 5514 8036 1121
Size: 30 193 2522 4454
Fragments arranged by size:
4454 2522 1101 957 864 420 291 222
193 177 138 30
BbvCI CC'TCA_GC
Cuts at: 566 1718 8179 566
Size: 1152 6461 3756
Fragments arranged by size:
6461 3756 1152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4554 4588 4699 4733 4819 4853 4554
Size: 34 111 34 86 34 11070
Fragments arranged by size:
11070 111 86 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9267 10028 11369 9267
Size: 761 1341 9267
Fragments arranged by size:
9267 1341 761
BclI T'GATC_A
Cuts at: 3960 4677 7572 8889 3960
Size: 717 2895 1317 6440
Fragments arranged by size:
6440 2895 1317 717
BglI GCCn_nnn'nGGC
Cuts at: 2900 4996 5624 6817 2900
Size: 2096 628 1193 7452
Fragments arranged by size:
7452 2096 1193 628
BglII A'GATC_T
Cuts at: 1678 1822 1978 2242 2275 3310 3439 5701 5773
Size: 144 156 264 33 1035 129 2262 72
Cuts at: 5773 6085 6199 6316 8884 10921 1678
Size: 312 114 117 2568 2037 2126
Fragments arranged by size:
2568 2262 2126 2037 1035 312 264 156
144 129 117 114 72 33
BlpI GC'TnA_GC
Cuts at: 1220 5346 8063 8481 1220
Size: 4126 2717 418 4108
Fragments arranged by size:
4126 4108 2717 418
Bme1580I G_kGCm'C
Cuts at: 579 888 4950 5001 7265 7613 7653 8994 9087
Size: 309 4062 51 2264 348 40 1341 93
Cuts at: 9087 10143 579
Size: 1056 1805
Fragments arranged by size:
4062 2264 1805 1341 1056 348 309 93
51 40
BmgBI CAC'GTC
Cuts at: 1699 2071 2104 2236 7559 1699
Size: 372 33 132 5323 5509
Fragments arranged by size:
5509 5323 372 132 33
BmrI ACTGGGnnnn_n'
Cuts at: 1341 1635 4748 5851 7655 8581 8662 8997 11141
Size: 294 3113 1103 1804 926 81 335 2144
Cuts at: 11141 1341
Size: 1569
Fragments arranged by size:
3113 2144 1804 1569 1103 926 335 294
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 732 734 2405 2960 3392 4619 4803 6032 9736
Size: 2 1671 555 432 1227 184 1229 3704
Cuts at: 9736 732
Size: 2365
Fragments arranged by size:
3704 2365 1671 1229 1227 555 432 184
2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 346 424 1424 1430 2070 2139 2541 3401
Size: 84 78 1000 6 640 69 402 860
Cuts at: 3401 3749 4025 4424 4778 4838 4970 5401 5699
Size: 348 276 399 354 60 132 431 298
Cuts at: 5699 6089 6161 6252 6308 6371 6407 7065 7071
Size: 390 72 91 56 63 36 658 6
Cuts at: 7071 7088 7155 7208 7415 7764 8337 8345 262
Size: 17 67 53 207 349 573 8 3286
Fragments arranged by size:
3286 1000 860 658 640 573 431 402
399 390 354 349 348 298 276 207
132 91 84 78 72 69 67 63
60 56 53 36 17 8 6 6
Bpu10I CC'TnA_GC
Cuts at: 566 602 1718 2465 2781 3506 8179 566
Size: 36 1116 747 316 725 4673 3756
Fragments arranged by size:
4673 3756 1116 747 725 316 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4718 5361 5421 5556 8545 9424 9916 10214 4718
Size: 643 60 135 2989 879 492 298 5873
Fragments arranged by size:
5873 2989 879 643 492 298 135 60
BsaI GGTCTCn'nnnn_
Cuts at: 430 803 854 947 1601 1718 1922 1955 2015
Size: 373 51 93 654 117 204 33 60
Cuts at: 2015 2186 2393 7457 7526 8363 11073 430
Size: 171 207 5064 69 837 2710 726
Fragments arranged by size:
5064 2710 837 726 654 373 207 204
171 117 93 69 60 51 33
BsaAI yAC'GTr
Cuts at: 543 8722 543
Size: 8179 3190
BsaBI GATnn'nnATC
Cuts at: 6 1632 1890 2964 5877 6054 6090 8883 10920
Size: 1626 258 1074 2913 177 36 2793 2037
Cuts at: 10920 6
Size: 455
Fragments arranged by size:
2913 2793 2037 1626 1074 455 258 177
36
BsaHI Gr'CG_yC
Cuts at: 133 237 2932 5169 5466 5616 6189 7887 8800
Size: 104 2695 2237 297 150 573 1698 913
Cuts at: 8800 9048 9750 11246 133
Size: 248 702 1496 256
Fragments arranged by size:
2695 2237 1698 1496 913 702 573 297
256 248 150 104
BsaWI w'CCGG_w
Cuts at: 1167 5190 5279 6069 7893 8558 9079 9757 10031
Size: 4023 89 790 1824 665 521 678 274
Cuts at: 10031 10178 10589 1167
Size: 147 411 1947
Fragments arranged by size:
4023 1947 1824 790 678 665 521 411
274 147 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1545 1575 6303 6333 7063 7093 7292 7322 8275
Size: 30 4728 30 730 30 199 30 953
Cuts at: 8275 8305 8335 8365 1545
Size: 30 30 30 4549
Fragments arranged by size:
4728 4549 953 730 199 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 343 448 710 909 1706 2615 2783 2895
Size: 105 105 262 199 797 909 168 112
Cuts at: 2895 3015 4608 4848 5598 6311 6374 6683 7698
Size: 120 1593 240 750 713 63 309 1015
Cuts at: 7698 7722 7725 7755 7758 7761 7938 7941 7956
Size: 24 3 30 3 3 177 3 15
Cuts at: 7956 7959 7962 7965 7968 7971 7983 7986 8010
Size: 3 3 3 3 3 12 3 24
Cuts at: 8010 8022 8025 8340 238
Size: 12 3 315 3267
Fragments arranged by size:
3267 1593 1015 909 797 750 713 315
309 262 240 199 177 168 120 112
105 105 63 30 24 24 15 12
12 3 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 982 1691 1806 2061 2094 2127 3754 6984 7014
Size: 709 115 255 33 33 1627 3230 30
Cuts at: 7014 7652 7846 7857 7905 8216 8296 8794 9552
Size: 638 194 11 48 311 80 498 758
Cuts at: 9552 10129 10625 982
Size: 577 496 1726
Fragments arranged by size:
3230 1726 1627 758 709 638 577 498
496 311 255 194 115 80 48 33
33 30 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1646 3236 7121 1646
Size: 1590 3885 5894
Fragments arranged by size:
5894 3885 1590
BsiEI CG_ry'CG
Cuts at: 104 8911 8957 10165 104
Size: 8807 46 1208 1308
Fragments arranged by size:
8807 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4622 8383 9161 9351 10143 4622
Size: 3761 778 190 792 5848
Fragments arranged by size:
5848 3761 792 778 190
BsmI GAATG_Cn'
Cuts at: 6515 6515
Size: 11369
BsmBI CGTCTCn'nnnn_
Cuts at: 2706 5793 2706
Size: 3087 8282
Bsp1286I G_dGCh'C
Cuts at: 579 888 2833 4622 4950 5001 5141 7265 7380
Size: 309 1945 1789 328 51 140 2124 115
Cuts at: 7380 7613 7653 7804 8383 8994 9087 9161 9351
Size: 233 40 151 579 611 93 74 190
Cuts at: 9351 9413 10143 579
Size: 62 730 1805
Fragments arranged by size:
2124 1945 1805 1789 730 611 579 328
309 233 190 151 140 115 93 74
62 51 40
BspEI T'CCGG_A
Cuts at: 1167 5190 7893 10589 1167
Size: 4023 2703 2696 1947
Fragments arranged by size:
4023 2703 2696 1947
BspHI T'CATG_A
Cuts at: 6758 10545 6758
Size: 3787 7582
BspMI ACCTGCnnnn'nnnn_
Cuts at: 692 1206 1443 1754 1844 1881 1937 1974 1997
Size: 514 237 311 90 37 56 37 23
Cuts at: 1997 2033 2321 2375 3506 4415 5589 6858 7644
Size: 36 288 54 1131 909 1174 1269 786
Cuts at: 7644 8100 8419 8633 8935 9316 692
Size: 456 319 214 302 381 2745
Fragments arranged by size:
2745 1269 1174 1131 909 786 514 456
381 319 311 302 288 237 214 90
56 54 37 37 36 23
BsrBI CCG'CTC
Cuts at: 50 204 1212 1706 1862 2078 2111 2295 5231
Size: 154 1008 494 156 216 33 184 2936
Cuts at: 5231 5390 5435 5630 5942 6128 6158 6194 6266
Size: 159 45 195 312 186 30 36 72
Cuts at: 6266 6685 7308 7695 8124 8256 8291 8348 9661
Size: 419 623 387 429 132 35 57 1313
Cuts at: 9661 9715 11199 11364 50
Size: 54 1484 165 55
Fragments arranged by size:
2936 1484 1313 1008 623 494 429 419
387 312 216 195 186 184 165 159
156 154 132 72 57 55 54 45
36 35 33 30
BsrDI GCAATG_nn'
Cuts at: 3790 9281 3790
Size: 5491 5878
BsrFI r'CCGG_y
Cuts at: 5519 5726 6562 7165 9367 9548 9757 5519
Size: 207 836 603 2202 181 209 7131
Fragments arranged by size:
7131 2202 836 603 209 207 181
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 11369
BssSI C'ACGA_G
Cuts at: 286 5408 9640 9998 286
Size: 5122 4232 358 1657
Fragments arranged by size:
5122 4232 1657 358
BstAPI GCAn_nnn'nTGC
Cuts at: 6814 8746 6814
Size: 1932 9437
BstBI TT'CG_AA
Cuts at: 9730 9730
Size: 11369
BstEII G'GTnAC_C
Cuts at: 5576 6072 5576
Size: 496 10873
BstXI CCAn_nnnn'nTGG
Cuts at: 1699 5687 6915 1699
Size: 3988 1228 6153
Fragments arranged by size:
6153 3988 1228
BstYI r'GATC_y
Cuts at: 1 1678 1801 1822 1978 2209 2242 2275 2506
Size: 1677 123 21 156 231 33 33 231
Cuts at: 2506 3199 3310 3439 4203 4237 4726 5482 5701
Size: 693 111 129 764 34 489 756 219
Cuts at: 5701 5773 5836 5878 5983 6055 6085 6199 6271
Size: 72 63 42 105 72 30 114 72
Cuts at: 6271 6316 8394 8861 8884 9219 9465 10477 10563
Size: 45 2078 467 23 335 246 1012 86
Cuts at: 10563 10575 10921 1
Size: 12 346 449
Fragments arranged by size:
2078 1677 1012 764 756 693 489 467
449 346 335 246 231 231 219 156
129 123 114 111 105 86 72 72
72 63 45 42 34 33 33 30
23 21 12
Bsu36I CC'TnA_GG
Cuts at: 3389 3623 4766 7823 8075 8161 3389
Size: 234 1143 3057 252 86 6597
Fragments arranged by size:
6597 3057 1143 252 234 86
BtgI C'CryG_G
Cuts at: 2401 2947 5686 8227 2401
Size: 546 2739 2541 5543
Fragments arranged by size:
5543 2739 2541 546
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 327 9319 9500 9663 327
Size: 8992 181 163 2033
Fragments arranged by size:
8992 2033 181 163
BtsI GCAGTG_nn'
Cuts at: 2145 4283 6872 7550 8256 8612 2145
Size: 2138 2589 678 706 356 4902
Fragments arranged by size:
4902 2589 2138 706 678 356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 7103 7138 7103
Size: 35 11334
DraI TTT'AAA
Cuts at: 8368 8368
Size: 11369
DraIII CAC_nnn'GTG
Cuts at: 4954 4954
Size: 11369
DrdI GACnn_nn'nnGTC
Cuts at: 2141 9075 9933 2141
Size: 6934 858 3577
Fragments arranged by size:
6934 3577 858
EaeI y'GGCC_r
Cuts at: 1500 1701 2073 2106 6905 6917 8954 9128 9519
Size: 201 372 33 4799 12 2037 174 391
Cuts at: 9519 9546 10891 1500
Size: 27 1345 1978
Fragments arranged by size:
4799 2037 1978 1345 391 372 201 174
33 27 12
EagI C'GGCC_G
Cuts at: 8954 8954
Size: 11369
EarI CTCTTCn'nnn_
Cuts at: 335 1419 1752 2262 2400 2913 3537 3809 4320
Size: 1084 333 510 138 513 624 272 511
Cuts at: 4320 4794 5616 7427 7743 7831 7912 7959 7989
Size: 474 822 1811 316 88 81 47 30
Cuts at: 7989 8004 8010 9392 9602 335
Size: 15 6 1382 210 2102
Fragments arranged by size:
2102 1811 1382 1084 822 624 513 511
510 474 333 316 272 210 138 88
81 47 30 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1896 2394 2558 5081 5562 6247 7713 9577 9887
Size: 498 164 2523 481 685 1466 1864 310
Cuts at: 9887 10033 11179 1896
Size: 146 1146 2086
Fragments arranged by size:
2523 2086 1864 1466 1146 685 498 481
310 164 146
EcoICRI GAG'CTC
Cuts at: 4620 8381 4620
Size: 3761 7608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 346 424 989 1424 1430 2070 2139 2273
Size: 84 78 565 435 6 640 69 134
Cuts at: 2273 2541 3401 3749 3863 3944 4025 4424 4778
Size: 268 860 348 114 81 81 399 354
Cuts at: 4778 4838 4934 4970 5075 5401 5699 5804 6089
Size: 60 96 36 105 326 298 105 285
Cuts at: 6089 6161 6252 6308 6371 6407 6441 7065 7071
Size: 72 91 56 63 36 34 624 6
Cuts at: 7071 7088 7155 7208 7415 7764 7855 8337 8345
Size: 17 67 53 207 349 91 482 8
Cuts at: 8345 9194 9626 10373 10641 262
Size: 849 432 747 268 990
Fragments arranged by size:
990 860 849 747 640 624 565 482
435 432 399 354 349 348 326 298
285 268 268 207 134 114 105 105
96 91 91 84 81 81 78 72
69 67 63 60 56 53 36 36
34 17 8 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1004 1542 3392 7514 1004
Size: 538 1850 4122 4859
Fragments arranged by size:
4859 4122 1850 538
EcoO109I rG'GnC_Cy
Cuts at: 1451 1556 1790 1856 1985 2759 2786 3020 4490
Size: 105 234 66 129 774 27 234 1470
Cuts at: 4490 4998 5044 5423 6122 6164 6589 7126 7649
Size: 508 46 379 699 42 425 537 523
Cuts at: 7649 7842 8129 8508 1451
Size: 193 287 379 4312
Fragments arranged by size:
4312 1470 774 699 537 523 508 425
379 379 287 234 234 193 129 105
66 46 42 27
EcoRI G'AATT_C
Cuts at: 3558 8373 3558
Size: 4815 6554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3739 6044 9388 3739
Size: 2305 3344 5720
Fragments arranged by size:
5720 3344 2305
FspI TGC'GCA
Cuts at: 9150 9150
Size: 11369
HaeII r_GCGC'y
Cuts at: 240 5346 6192 6842 7926 8803 9051 10073 11052
Size: 5106 846 650 1084 877 248 1022 979
Cuts at: 11052 240
Size: 557
Fragments arranged by size:
5106 1084 1022 979 877 846 650 557
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 2737 2769 3295 3327 3359 3418 3450
Size: 32 2167 32 526 32 32 59 32
Cuts at: 3450 3945 3977 4855 4887 5136 5168 5595 5627
Size: 495 32 878 32 249 32 427 32
Cuts at: 5627 6567 6599 8113 8145 8158 8190 8275 8307
Size: 940 32 1514 32 13 32 85 32
Cuts at: 8307 9211 9241 9243 9273 538
Size: 904 30 2 30 2634
Fragments arranged by size:
2634 2167 1514 940 904 878 526 495
427 249 85 59 32 32 32 32
32 32 32 32 32 32 32 32
32 30 30 13 2
HincII GTy'rAC
Cuts at: 4863 5495 8408 4863
Size: 632 2913 7824
Fragments arranged by size:
7824 2913 632
HindIII A'AGCT_T
Cuts at: 2223 4568 2223
Size: 2345 9024
KasI G'GCGC_C
Cuts at: 236 6188 8799 9047 236
Size: 5952 2611 248 2558
Fragments arranged by size:
5952 2611 2558 248
KpnI G_GTAC'C
Cuts at: 8389 8389
Size: 11369
MluI A'CGCG_T
Cuts at: 10802 10802
Size: 11369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1327 5632 6883 10040 10224 1327
Size: 4305 1251 3157 184 2472
Fragments arranged by size:
4305 3157 2472 1251 184
MscI TGG'CCA
Cuts at: 6919 9130 6919
Size: 2211 9158
MslI CAynn'nnrTG
Cuts at: 1668 7619 9358 9485 1668
Size: 5951 1739 127 3552
Fragments arranged by size:
5951 3552 1739 127
MspA1I CmG'CkG
Cuts at: 146 457 4194 5514 6895 6904 6934 6988 7018
Size: 311 3737 1320 1381 9 30 54 30
Cuts at: 7018 8480 8794 9154 10167 10412 146
Size: 1462 314 360 1013 245 1103
Fragments arranged by size:
3737 1462 1381 1320 1103 1013 360 314
311 245 54 30 30 9
NaeI GCC'GGC
Cuts at: 6564 7167 9550 6564
Size: 603 2383 8383
Fragments arranged by size:
8383 2383 603
NarI GG'CG_CC
Cuts at: 237 6189 8800 9048 237
Size: 5952 2611 248 2558
Fragments arranged by size:
5952 2611 2558 248
NgoMIV G'CCGG_C
Cuts at: 6562 7165 9548 6562
Size: 603 2383 8383
Fragments arranged by size:
8383 2383 603
NspI r_CATG'y
Cuts at: 1408 6474 7144 7587 8422 8922 9829 1408
Size: 5066 670 443 835 500 907 2948
Fragments arranged by size:
5066 2948 907 835 670 500 443
PasI CC'CwG_GG
Cuts at: 421 442 4704 4943 7256 7603 421
Size: 21 4262 239 2313 347 4187
Fragments arranged by size:
4262 4187 2313 347 239 21
PciI A'CATG_T
Cuts at: 9825 9825
Size: 11369
PflMI CCAn_nnn'nTGG
Cuts at: 8116 8116
Size: 11369
PfoI T'CCnGG_A
Cuts at: 278 4440 4497 5599 5812 5986 6166 6643 8263
Size: 4162 57 1102 213 174 180 477 1620
Cuts at: 8263 278
Size: 3384
Fragments arranged by size:
4162 3384 1620 1102 477 213 180 174
57
PmeI GTTT'AAAC
Cuts at: 8368 8368
Size: 11369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4584 4616 4704 4736 5412 5444 10508 10540 4584
Size: 32 88 32 676 32 5064 32 5413
Fragments arranged by size:
5413 5064 676 88 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1451 1556 1790 1856 1985 2759 2786 3020 4490
Size: 105 234 66 129 774 27 234 1470
Cuts at: 4490 5423 6122 6164 6589 7126 7842 8129 1451
Size: 933 699 42 425 537 716 287 4691
Fragments arranged by size:
4691 1470 933 774 716 699 537 425
287 234 234 129 105 66 42 27
PshAI GACnn'nnGTC
Cuts at: 702 2007 5766 702
Size: 1305 3759 6305
Fragments arranged by size:
6305 3759 1305
PspOMI G'GGCC_C
Cuts at: 884 4997 7649 884
Size: 4113 2652 4604
Fragments arranged by size:
4604 4113 2652
PspXI vC'TCGA_Gb
Cuts at: 319 4470 319
Size: 4151 7218
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1812 1844 4887 4919 1812
Size: 32 3043 32 8262
Fragments arranged by size:
8262 3043 32 32
PstI C_TGCA'G
Cuts at: 957 3368 6499 6557 6810 6900 7682 8416 9101
Size: 2411 3131 58 253 90 782 734 685
Cuts at: 9101 957
Size: 3225
Fragments arranged by size:
3225 3131 2411 782 734 685 253 90
58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11369
PvuII CAG'CTG
Cuts at: 4194 6895 6988 7018 8794 9154 4194
Size: 2701 93 30 1776 360 6409
Fragments arranged by size:
6409 2701 1776 360 93 30
RsrII CG'GwC_CG
Cuts at: 9564 9564
Size: 11369
SacI G_AGCT'C
Cuts at: 4622 8383 4622
Size: 3761 7608
SalI G'TCGA_C
Cuts at: 8406 8406
Size: 11369
SanDI GG'GwC_CC
Cuts at: 1451 7842 1451
Size: 6391 4978
SapI GCTCTTCn'nnn_
Cuts at: 335 1419 9392 9602 335
Size: 1084 7973 210 2102
Fragments arranged by size:
7973 2102 1084 210
SbfI CC_TGCA'GG
Cuts at: 8416 8416
Size: 11369
ScaI AGT'ACT
Cuts at: 10886 10886
Size: 11369
SexAI A'CCwGG_T
Cuts at: 1044 7118 1044
Size: 6074 5295
SfcI C'TryA_G
Cuts at: 33 953 3364 3545 6495 6553 6806 6896 7511
Size: 920 2411 181 2950 58 253 90 615
Cuts at: 7511 7678 8412 9097 10090 10281 33
Size: 167 734 685 993 191 1121
Fragments arranged by size:
2950 2411 1121 993 920 734 685 615
253 191 181 167 90 58
SfoI GGC'GCC
Cuts at: 238 6190 8801 9049 238
Size: 5952 2611 248 2558
Fragments arranged by size:
5952 2611 2558 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11369
SgrAI Cr'CCGG_yG
Cuts at: 5726 5726
Size: 11369
SmaI CCC'GGG
Cuts at: 139 8391 139
Size: 8252 3117
SmlI C'TyrA_G
Cuts at: 319 1263 4350 4470 4733 5376 5436 5571 5705
Size: 944 3087 120 263 643 60 135 134
Cuts at: 5705 8524 9439 9931 10193 319
Size: 2819 915 492 262 1495
Fragments arranged by size:
3087 2819 1495 944 915 643 492 263
262 135 134 120 60
SnaBI TAC'GTA
Cuts at: 543 543
Size: 11369
SpeI A'CTAG_T
Cuts at: 67 3180 67
Size: 3113 8256
SphI G_CATG'C
Cuts at: 1408 7587 8422 8922 1408
Size: 6179 835 500 3855
Fragments arranged by size:
6179 3855 835 500
SspI AAT'ATT
Cuts at: 7161 7161
Size: 11369
StuI AGG'CCT
Cuts at: 1297 3693 3939 8080 1297
Size: 2396 246 4141 4586
Fragments arranged by size:
4586 4141 2396 246
StyI C'CwwG_G
Cuts at: 977 4585 5272 6522 7022 7187 8503 8857 977
Size: 3608 687 1250 500 165 1316 354 3489
Fragments arranged by size:
3608 3489 1316 1250 687 500 354 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 929 1583 1910 1943 2174 2502 2556 3389 4829
Size: 654 327 33 231 328 54 833 1440
Cuts at: 4829 4956 6555 7121 7331 8162 8952 9085 9544
Size: 127 1599 566 210 831 790 133 459
Cuts at: 9544 9753 929
Size: 209 2545
Fragments arranged by size:
2545 1599 1440 833 831 790 654 566
459 328 327 231 210 209 133 127
54 33
TatI w'GTAC_w
Cuts at: 110 856 3681 10884 110
Size: 746 2825 7203 595
Fragments arranged by size:
7203 2825 746 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1786 1818 5716 5748 6239 6271 7292 7324 8335
Size: 32 3898 32 491 32 1021 32 1011
Cuts at: 8335 8367 10886 10918 1786
Size: 32 2519 32 2237
Fragments arranged by size:
3898 2519 2237 1021 1011 491 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2549 2606 3068 3341 5148 7557 9166 2549
Size: 57 462 273 1807 2409 1609 4752
Fragments arranged by size:
4752 2409 1807 1609 462 273 57
XbaI T'CTAG_A
Cuts at: 1681 1798 1831 1864 1924 1957 2053 2119 2251
Size: 117 33 33 60 33 96 66 132
Cuts at: 2251 2530 2767 4212 4528 5260 5698 5917 5950
Size: 279 237 1445 316 732 438 219 33
Cuts at: 5950 6196 6532 7393 8400 1681
Size: 246 336 861 1007 4650
Fragments arranged by size:
4650 1445 1007 861 732 438 336 316
279 246 237 219 132 117 96 66
60 33 33 33 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6933 7215 8864 6933
Size: 282 1649 9438
Fragments arranged by size:
9438 1649 282
XhoI C'TCGA_G
Cuts at: 319 4350 4470 5705 319
Size: 4031 120 1235 5983
Fragments arranged by size:
5983 4031 1235 120
XmaI C'CCGG_G
Cuts at: 137 8389 137
Size: 8252 3117
XmnI GAAnn'nnTTC
Cuts at: 993 2895 4365 4719 6357 6402 993
Size: 1902 1470 354 1638 45 5960
Fragments arranged by size:
5960 1902 1638 1470 354 45
ZraI GAC'GTC
Cuts at: 2933 5170 5467 7888 2933
Size: 2237 297 2421 6414
Fragments arranged by size:
6414 2421 2237 297
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BfrBI BmtI BsiWI BssHII BstZ17I
ClaI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI SacII
SfiI SrfI SwaI