Restriction map for
pF1KA0318
(Circular) (Six-base) MAPSORT of: pF1KA0318.seq Check: 1745 from: 1 to: 6269
pF1KA0318 6269 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1304 1304
Size: 6269
AccI GT'mk_AC
Cuts at: 3307 3307
Size: 6269
Acc65I G'GTAC_C
Cuts at: 3285 3285
Size: 6269
AclI AA'CG_TT
Cuts at: 1676 6109 1676
Size: 4433 1836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1739 4094 4526 4879 5541 1739
Size: 2355 432 353 662 2467
Fragments arranged by size:
2467 2355 662 432 353
AfeI AGC'GCT
Cuts at: 426 2535 426
Size: 2109 4160
AflIII A'CryG_T
Cuts at: 3621 5421 5702 3621
Size: 1800 281 4188
Fragments arranged by size:
4188 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 813 813
Size: 6269
AleI CACnn'nnGTG
Cuts at: 620 1750 2095 620
Size: 1130 345 4794
Fragments arranged by size:
4794 1130 345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1225 2009 1225
Size: 784 5485
AlwNI CAG_nnn'CTG
Cuts at: 138 162 1843 2488 2629 2716 3574 5012 138
Size: 24 1681 645 141 87 858 1438 1395
Fragments arranged by size:
1681 1438 1395 858 645 141 87 24
ApaI G_GGCC'C
Cuts at: 2041 2041
Size: 6269
ApaLI G'TGCA_C
Cuts at: 1251 1994 5107 1251
Size: 743 3113 2413
Fragments arranged by size:
3113 2413 743
ApoI r'AATT_y
Cuts at: 576 3273 5622 5878 576
Size: 2697 2349 256 967
Fragments arranged by size:
2697 2349 967 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6269
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6269
AvaI C'yCGr_G
Cuts at: 272 603 1855 1884 2126 3289 272
Size: 331 1252 29 242 1163 3252
Fragments arranged by size:
3252 1252 1163 331 242 29
BamHI G'GATC_C
Cuts at: 3294 3294
Size: 6269
BanI G'GyrC_C
Cuts at: 777 1071 1866 2089 2263 2638 3285 3699 3947
Size: 294 795 223 174 375 647 414 248
Cuts at: 3947 3982 777
Size: 35 3064
Fragments arranged by size:
3064 795 647 414 375 294 248 223
174 35
BanII G_rGCy'C
Cuts at: 755 1064 1862 2041 2651 3283 4313 755
Size: 309 798 179 610 632 1030 2711
Fragments arranged by size:
2711 1030 798 632 610 309 179
BbeI G_GCGC'C
Cuts at: 2267 3703 3951 2267
Size: 1436 248 4585
Fragments arranged by size:
4585 1436 248
BbsI GAAGACnn'nnnn_
Cuts at: 995 2172 995
Size: 1177 5092
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 931 965 90
Size: 34 807 34 5394
Fragments arranged by size:
5394 807 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4167 5223 6269 4167
Size: 1056 1046 4167
Fragments arranged by size:
4167 1056 1046
BclI T'GATC_A
Cuts at: 3789 3789
Size: 6269
BglI GCCn_nnn'nGGC
Cuts at: 1656 2131 1656
Size: 475 5794
BglII A'GATC_T
Cuts at: 547 1319 3784 5821 547
Size: 772 2465 2037 995
Fragments arranged by size:
2465 2037 995 772
BlpI GC'TnA_GC
Cuts at: 677 3381 677
Size: 2704 3565
Bme1580I G_kGCm'C
Cuts at: 782 1255 1998 2018 2041 2092 2811 3137 3894
Size: 473 743 20 23 51 719 326 757
Cuts at: 3894 3987 5111 782
Size: 93 1124 1940
Fragments arranged by size:
1940 1124 757 743 719 473 326 93
51 23 20
BmgBI CAC'GTC
Cuts at: 966 1275 1368 2450 966
Size: 309 93 1082 4785
Fragments arranged by size:
4785 1082 309 93
BmrI ACTGGGnnnn_n'
Cuts at: 3481 3562 3897 6041 3481
Size: 81 335 2144 3709
Fragments arranged by size:
3709 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 772 4636 772
Size: 3864 2405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 904 1528 1650 1798 1943 2044 2228 2588
Size: 603 624 122 148 145 101 184 360
Cuts at: 2588 301
Size: 3982
Fragments arranged by size:
3982 624 603 360 184 148 145 122
101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2449 3191 3445 4324 5038 5336 2449
Size: 742 254 879 714 298 3382
Fragments arranged by size:
3382 879 742 714 298 254
BsaI GGTCTCn'nnnn_
Cuts at: 1626 1629 1881 2585 2844 5973 1626
Size: 3 252 704 259 3129 1922
Fragments arranged by size:
3129 1922 704 259 252 3
BsaAI yAC'GTr
Cuts at: 3622 3622
Size: 6269
BsaBI GATnn'nnATC
Cuts at: 6 546 3783 5820 6
Size: 540 3237 2037 455
Fragments arranged by size:
3237 2037 540 455
BsaHI Gr'CG_yC
Cuts at: 1648 2231 2264 2952 3700 3948 4650 6146 1648
Size: 583 33 688 748 248 702 1496 1771
Fragments arranged by size:
1771 1496 748 702 688 583 248 33
BsaWI w'CCGG_w
Cuts at: 529 3458 3979 4657 5068 5215 5489 529
Size: 2929 521 678 411 147 274 1309
Fragments arranged by size:
2929 1309 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 484 514 895 925 1189 1219 2383 2413 484
Size: 30 381 30 264 30 1164 30 4340
Fragments arranged by size:
4340 1164 381 264 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 745 1399 1402 2239 2271 2395 2404 2407 2737
Size: 654 3 837 32 124 9 3 330
Cuts at: 2737 2740 2878 745
Size: 3 138 4136
Fragments arranged by size:
4136 837 654 330 138 124 32 9
3 3 3
BseYI C'CCAG_C
Cuts at: 318 361 834 1055 1143 1328 1566 1597 2062
Size: 43 473 221 88 185 238 31 465
Cuts at: 2062 2440 2481 3694 4452 5117 5525 318
Size: 378 41 1213 758 665 408 1062
Fragments arranged by size:
1213 1062 758 665 473 465 408 378
238 221 185 88 43 41 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1176 1231 1877 823
Size: 353 55 646 5215
Fragments arranged by size:
5215 646 353 55
BsiEI CG_ry'CG
Cuts at: 104 449 1751 2053 3811 3857 5087 104
Size: 345 1302 302 1758 46 1230 1286
Fragments arranged by size:
1758 1302 1286 1230 345 302 46
BsiHKAI G_wGCw'C
Cuts at: 755 896 1255 1862 1949 1998 2651 3283 4061
Size: 141 359 607 87 49 653 632 778
Cuts at: 4061 4251 5111 755
Size: 190 860 1913
Fragments arranged by size:
1913 860 778 653 632 607 359 190
141 87 49
BsmBI CGTCTCn'nnnn_
Cuts at: 2321 2321
Size: 6269
Bsp1286I G_dGCh'C
Cuts at: 755 782 896 1064 1255 1862 1949 1998 2018
Size: 27 114 168 191 607 87 49 20
Cuts at: 2018 2041 2092 2651 2811 3137 3283 3894 3987
Size: 23 51 559 160 326 146 611 93
Cuts at: 3987 4061 4251 4313 5111 755
Size: 74 190 62 798 1913
Fragments arranged by size:
1913 798 611 607 559 326 191 190
168 160 146 114 93 87 74 62
51 49 27 23 20
BspHI T'CATG_A
Cuts at: 383 4701 383
Size: 4318 1951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 323 1304 1899 2150 3319 3533 3835 4216
Size: 51 981 595 251 1169 214 302 381
Cuts at: 4216 272
Size: 2325
Fragments arranged by size:
2325 1169 981 595 381 302 251 214
51
BsrBI CCG'CTC
Cuts at: 50 2691 4561 4615 6099 6264 50
Size: 2641 1870 54 1484 165 55
Fragments arranged by size:
2641 1870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4181 4181
Size: 6269
BsrFI r'CCGG_y
Cuts at: 1681 4267 4448 1681
Size: 2586 181 3502
Fragments arranged by size:
3502 2586 181
BssSI C'ACGA_G
Cuts at: 4540 5248 4540
Size: 708 5561
BstAPI GCAn_nnn'nTGC
Cuts at: 138 1843 3646 138
Size: 1705 1803 2761
Fragments arranged by size:
2761 1803 1705
BstBI TT'CG_AA
Cuts at: 4630 4630
Size: 6269
BstEII G'GTnAC_C
Cuts at: 1602 1602
Size: 6269
BstXI CCAn_nnnn'nTGG
Cuts at: 792 1082 2121 792
Size: 290 1039 4940
Fragments arranged by size:
4940 1039 290
BstYI r'GATC_y
Cuts at: 1 526 547 1319 2660 3294 3761 3784 4119
Size: 525 21 772 1341 634 467 23 335
Cuts at: 4119 4365 4671 4683 4769 5821 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1341 1052 772 634 525 467 449 335
306 246 86 23 21 12
BtgI C'CryG_G
Cuts at: 353 1492 1733 1751 1826 2033 2120 2322 2526
Size: 1139 241 18 75 207 87 202 204
Cuts at: 2526 2793 353
Size: 267 3829
Fragments arranged by size:
3829 1139 267 241 207 204 202 87
75 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4219 4400 4563 4219
Size: 181 163 5925
Fragments arranged by size:
5925 181 163
BtsI GCAGTG_nn'
Cuts at: 1219 1255 3512 1219
Size: 36 2257 3976
Fragments arranged by size:
3976 2257 36
DraI TTT'AAA
Cuts at: 2739 3268 2739
Size: 529 5740
DrdI GACnn_nn'nnGTC
Cuts at: 3172 3975 5319 3172
Size: 803 1344 4122
Fragments arranged by size:
4122 1344 803
EaeI y'GGCC_r
Cuts at: 762 1472 1748 2147 3854 4028 4419 4446 5791
Size: 710 276 399 1707 174 391 27 1345
Cuts at: 5791 762
Size: 1240
Fragments arranged by size:
1707 1345 1240 710 399 391 276 174
27
EagI C'GGCC_G
Cuts at: 1748 3854 1748
Size: 2106 4163
EarI CTCTTCn'nnn_
Cuts at: 224 2612 2639 3238 4292 4502 224
Size: 2388 27 599 1054 210 1991
Fragments arranged by size:
2388 1991 1054 599 210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 492 1314 1800 4477 5219 5365 6079 492
Size: 822 486 2677 742 146 714 682
Fragments arranged by size:
2677 822 742 714 682 486 146
EcoICRI GAG'CTC
Cuts at: 753 1860 2649 3281 753
Size: 1107 789 632 3741
Fragments arranged by size:
3741 1107 789 632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 904 1528 1650 1739 1798 1943 2044 2228
Size: 603 624 122 89 59 145 101 184
Cuts at: 2228 2588 4094 4526 4879 5541 301
Size: 360 1506 432 353 662 1029
Fragments arranged by size:
1506 1029 662 624 603 432 360 353
184 145 122 101 89 59
EcoNI CCTnn'n_nnAGG
Cuts at: 1381 1381
Size: 6269
EcoO109I rG'GnC_Cy
Cuts at: 159 276 956 1561 1954 2038 2549 3408 159
Size: 117 680 605 393 84 511 859 3020
Fragments arranged by size:
3020 859 680 605 511 393 117 84
EcoRI G'AATT_C
Cuts at: 576 3273 576
Size: 2697 3572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 531 1548 3196 4288 531
Size: 1017 1648 1092 2512
Fragments arranged by size:
2512 1648 1092 1017
FspI TGC'GCA
Cuts at: 4050 4050
Size: 6269
HaeII r_GCGC'y
Cuts at: 428 2267 2537 3703 3951 5181 5952 428
Size: 1839 270 1166 248 1230 771 745
Fragments arranged by size:
1839 1230 1166 771 745 270 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 799 831 881 895 913 927 977
Size: 32 283 32 50 14 18 14 50
Cuts at: 977 1009 2859 2891 3178 3210 4111 4141 4143
Size: 32 1850 32 287 32 901 30 2
Cuts at: 4143 4173 484
Size: 30 2580
Fragments arranged by size:
2580 1850 901 287 283 50 50 32
32 32 32 32 30 30 18 14
14 2
HincII GTy'rAC
Cuts at: 3308 3308
Size: 6269
KasI G'GCGC_C
Cuts at: 2263 3699 3947 2263
Size: 1436 248 4585
Fragments arranged by size:
4585 1436 248
KpnI G_GTAC'C
Cuts at: 3289 3289
Size: 6269
MluI A'CGCG_T
Cuts at: 5702 5702
Size: 6269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 125 809 5028 5212 125
Size: 684 4219 184 1182
Fragments arranged by size:
4219 1182 684 184
MscI TGG'CCA
Cuts at: 764 1474 4030 764
Size: 710 2556 3003
Fragments arranged by size:
3003 2556 710
MslI CAynn'nnrTG
Cuts at: 620 926 1080 1207 1750 2095 4258 4385 620
Size: 306 154 127 543 345 2163 127 2504
Fragments arranged by size:
2504 2163 543 345 306 154 127 127
MspA1I CmG'CkG
Cuts at: 138 1506 2415 2444 3380 3694 4054 4838 5083
Size: 1368 909 29 936 314 360 784 245
Cuts at: 5083 138
Size: 1324
Fragments arranged by size:
1368 1324 936 909 784 360 314 245
29
NaeI GCC'GGC
Cuts at: 4450 4450
Size: 6269
NarI GG'CG_CC
Cuts at: 2264 3700 3948 2264
Size: 1436 248 4585
Fragments arranged by size:
4585 1436 248
NcoI C'CATG_G
Cuts at: 2120 2322 2120
Size: 202 6067
NgoMIV G'CCGG_C
Cuts at: 4448 4448
Size: 6269
NspI r_CATG'y
Cuts at: 2022 3322 3822 5425 2022
Size: 1300 500 1603 2866
Fragments arranged by size:
2866 1603 1300 500
PasI CC'CwG_GG
Cuts at: 1815 1815
Size: 6269
PciI A'CATG_T
Cuts at: 5421 5421
Size: 6269
PflMI CCAn_nnn'nTGG
Cuts at: 162 162
Size: 6269
PfoI T'CCnGG_A
Cuts at: 899 1627 899
Size: 728 5541
PmeI GTTT'AAAC
Cuts at: 3268 3268
Size: 6269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 721 753 1375 1407 2470 2502 4715 4747 721
Size: 32 622 32 1063 32 2213 32 2243
Fragments arranged by size:
2243 2213 1063 622 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 276 956 1561 1954 2549 276
Size: 680 605 393 595 3996
Fragments arranged by size:
3996 680 605 595 393
PspOMI G'GGCC_C
Cuts at: 2037 2037
Size: 6269
PstI C_TGCA'G
Cuts at: 143 206 1250 1560 1839 2493 2721 3316 4001
Size: 63 1044 310 279 654 228 595 685
Cuts at: 4001 143
Size: 2411
Fragments arranged by size:
2411 1044 685 654 595 310 279 228
63
PvuI CG_AT'CG
Cuts at: 104 449 104
Size: 345 5924
PvuII CAG'CTG
Cuts at: 138 2415 3694 4054 138
Size: 2277 1279 360 2353
Fragments arranged by size:
2353 2277 1279 360
RsrII CG'GwC_CG
Cuts at: 4464 4464
Size: 6269
SacI G_AGCT'C
Cuts at: 755 1862 2651 3283 755
Size: 1107 789 632 3741
Fragments arranged by size:
3741 1107 789 632
SalI G'TCGA_C
Cuts at: 3306 3306
Size: 6269
SanDI GG'GwC_CC
Cuts at: 956 1954 956
Size: 998 5271
SapI GCTCTTCn'nnn_
Cuts at: 2639 4292 4502 2639
Size: 1653 210 4406
Fragments arranged by size:
4406 1653 210
SbfI CC_TGCA'GG
Cuts at: 1560 3316 1560
Size: 1756 4513
ScaI AGT'ACT
Cuts at: 5786 5786
Size: 6269
SfcI C'TryA_G
Cuts at: 33 139 202 633 1246 1359 1556 1835 2489
Size: 106 63 431 613 113 197 279 654
Cuts at: 2489 2717 3312 3997 4965 5156 33
Size: 228 595 685 968 191 1146
Fragments arranged by size:
1146 968 685 654 613 595 431 279
228 197 191 113 106 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 2131 2131
Size: 6269
SfoI GGC'GCC
Cuts at: 2265 3701 3949 2265
Size: 1436 248 4585
Fragments arranged by size:
4585 1436 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6269
SmaI CCC'GGG
Cuts at: 274 2128 3291 274
Size: 1854 1163 3252
Fragments arranged by size:
3252 1854 1163
SmlI C'TyrA_G
Cuts at: 2464 3206 3424 4339 5053 5315 2464
Size: 742 218 915 714 262 3418
Fragments arranged by size:
3418 915 742 714 262 218
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6269
SphI G_CATG'C
Cuts at: 3322 3822 3322
Size: 500 5769
StuI AGG'CCT
Cuts at: 1531 1978 3128 1531
Size: 447 1150 4672
Fragments arranged by size:
4672 1150 447
StyI C'CwwG_G
Cuts at: 212 1784 2114 2120 2322 3403 3757 212
Size: 1572 330 6 202 1081 354 2724
Fragments arranged by size:
2724 1572 1081 354 330 202 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 672 2076 3852 3985 4444 4653 310
Size: 362 1404 1776 133 459 209 1926
Fragments arranged by size:
1926 1776 1404 459 362 209 133
TatI w'GTAC_w
Cuts at: 5784 5784
Size: 6269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 234 266 3231 3263 5786 5818 234
Size: 32 2965 32 2523 32 685
Fragments arranged by size:
2965 2523 685 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1399 4066 1399
Size: 2667 3602
XbaI T'CTAG_A
Cuts at: 3300 3300
Size: 6269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3764 3764
Size: 6269
XmaI C'CCGG_G
Cuts at: 272 2126 3289 272
Size: 1854 1163 3252
Fragments arranged by size:
3252 1854 1163
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AloI AscI AvrII BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmI BspEI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI