Restriction map for
pF1KA0310
(Circular) (Six-base) MAPSORT of: pF1KA0310.seq Check: 901 from: 1 to: 10184
pF1KA0310 10184 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3021 4863 5742 5753 3021
Size: 1842 879 11 7452
Fragments arranged by size:
7452 1842 879 11
AatII G_ACGT'C
Cuts at: 5261 6695 5261
Size: 1434 8750
AccI GT'mk_AC
Cuts at: 282 3425 3680 5141 7222 282
Size: 3143 255 1461 2081 3244
Fragments arranged by size:
3244 3143 2081 1461 255
Acc65I G'GTAC_C
Cuts at: 7200 7200
Size: 10184
AclI AA'CG_TT
Cuts at: 7178 10024 7178
Size: 2846 7338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 578 827 1196 1766 2216 2500 3845 4496
Size: 96 249 369 570 450 284 1345 651
Cuts at: 4496 8009 8441 9188 9456 482
Size: 3513 432 747 268 1210
Fragments arranged by size:
3513 1345 1210 747 651 570 450 432
369 284 268 249 96
AflII C'TTAA_G
Cuts at: 3099 3099
Size: 10184
AflIII A'CryG_T
Cuts at: 1356 3128 4568 6647 7536 8640 9617 1356
Size: 1772 1440 2079 889 1104 977 1923
Fragments arranged by size:
2079 1923 1772 1440 1104 977 889
AgeI A'CCGG_T
Cuts at: 1944 8572 1944
Size: 6628 3556
AhdI GACnn_n'nnGTC
Cuts at: 1668 2713 4701 5262 1668
Size: 1045 1988 561 6590
Fragments arranged by size:
6590 1988 1045 561
AleI CACnn'nnGTG
Cuts at: 1447 2995 3028 3938 5582 5730 1447
Size: 1548 33 910 1644 148 5901
Fragments arranged by size:
5901 1644 1548 910 148 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1465 3046 3191 7131 1465
Size: 1581 145 3940 4518
Fragments arranged by size:
4518 3940 1581 145
AlwNI CAG_nnn'CTG
Cuts at: 556 570 1376 1731 3288 3441 3541 3573 5481
Size: 14 806 355 1557 153 100 32 1908
Cuts at: 5481 6079 6883 7489 9056 556
Size: 598 804 606 1567 1684
Fragments arranged by size:
1908 1684 1567 1557 806 804 606 598
355 153 100 32 14
ApaI G_GGCC'C
Cuts at: 886 1580 2252 6044 7066 886
Size: 694 672 3792 1022 4004
Fragments arranged by size:
4004 3792 1022 694 672
ApaLI G'TGCA_C
Cuts at: 1654 2117 2164 8954 1654
Size: 463 47 6790 2884
Fragments arranged by size:
6790 2884 463 47
ApoI r'AATT_y
Cuts at: 1495 4438 4722 6240 6253 6531 7188 9537 9793
Size: 2943 284 1518 13 278 657 2349 256
Cuts at: 9793 1495
Size: 1886
Fragments arranged by size:
2943 2349 1886 1518 657 284 278 256
13
AseI AT'TA_AT
Cuts at: 20 4643 20
Size: 4623 5561
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10184
AvaI C'yCGr_G
Cuts at: 780 1121 1554 1768 1887 2218 3754 3760 3776
Size: 341 433 214 119 331 1536 6 16
Cuts at: 3776 4039 4599 4948 5084 5934 5940 6036 6273
Size: 263 560 349 136 850 6 96 237
Cuts at: 6273 6324 7204 780
Size: 51 880 3760
Fragments arranged by size:
3760 1536 880 850 560 433 349 341
331 263 237 214 136 119 96 51
16 6 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3822 3855 3822
Size: 33 10151
BamHI G'GATC_C
Cuts at: 264 603 5398 7209 264
Size: 339 4795 1811 3239
Fragments arranged by size:
4795 3239 1811 339
BanI G'GyrC_C
Cuts at: 828 1449 2187 3022 3030 3207 3291 4324 5485
Size: 621 738 835 8 177 84 1033 1161
Cuts at: 5485 5910 6201 6282 7200 7614 7862 7897 828
Size: 425 291 81 918 414 248 35 3115
Fragments arranged by size:
3115 1161 1033 918 835 738 621 425
414 291 248 177 84 81 35 8
BanII G_rGCy'C
Cuts at: 434 653 886 1058 1221 1532 1580 1632 1894
Size: 219 233 172 163 311 48 52 262
Cuts at: 1894 2252 2269 2730 3087 3279 3761 5805 5883
Size: 358 17 461 357 192 482 2044 78
Cuts at: 5883 5926 5941 6044 6101 6175 6639 6681 6896
Size: 43 15 103 57 74 464 42 215
Cuts at: 6896 7066 7135 7198 8228 434
Size: 170 69 63 1030 2390
Fragments arranged by size:
2390 2044 1030 482 464 461 358 357
311 262 233 219 215 192 172 170
163 103 78 74 69 63 57 52
48 43 42 17 15
BbeI G_GCGC'C
Cuts at: 3211 6205 7618 7866 3211
Size: 2994 1413 248 5529
Fragments arranged by size:
5529 2994 1413 248
BbsI GAAGACnn'nnnn_
Cuts at: 3077 4705 3077
Size: 1628 8556
BbvCI CC'TCA_GC
Cuts at: 722 2366 4216 5961 722
Size: 1644 1850 1745 4945
Fragments arranged by size:
4945 1850 1745 1644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3856 3890 6264 6298 7033 7067 90
Size: 34 3732 34 2374 34 735 34 3207
Fragments arranged by size:
3732 3207 2374 735 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2219 3823 8082 8843 10184 2193
Size: 26 1604 4259 761 1341 2193
Fragments arranged by size:
4259 2193 1604 1341 761 26
BclI T'GATC_A
Cuts at: 2607 5639 7704 2607
Size: 3032 2065 5087
Fragments arranged by size:
5087 3032 2065
BglI GCCn_nnn'nGGC
Cuts at: 783 2456 3263 3450 3765 6039 6105 783
Size: 1673 807 187 315 2274 66 4862
Fragments arranged by size:
4862 2274 1673 807 315 187 66
BglII A'GATC_T
Cuts at: 7699 9736 7699
Size: 2037 8147
BlpI GC'TnA_GC
Cuts at: 1713 2600 2627 6229 7100 7296 1713
Size: 887 27 3602 871 196 4601
Fragments arranged by size:
4601 3602 887 871 196 27
Bme1580I G_kGCm'C
Cuts at: 833 886 1188 1580 1601 1658 2121 2168 2190
Size: 53 302 392 21 57 463 47 22
Cuts at: 2190 2252 6044 7066 7809 7902 8958 833
Size: 62 3792 1022 743 93 1056 2059
Fragments arranged by size:
3792 2059 1056 1022 743 463 392 302
93 62 57 53 47 22 21
BmgBI CAC'GTC
Cuts at: 4571 4571
Size: 10184
BmrI ACTGGGnnnn_n'
Cuts at: 316 539 929 1050 1168 3930 5469 5661 6016
Size: 223 390 121 118 2762 1539 192 355
Cuts at: 6016 7396 7477 7812 9956 316
Size: 1380 81 335 2144 544
Fragments arranged by size:
2762 2144 1539 1380 544 390 355 335
223 192 121 118 81
BmtI G_CTAG'C
Cuts at: 191 191
Size: 10184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1284 8551 1284
Size: 7267 2917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 1305 2104 2372 2570 2703 3873 4080 4277
Size: 1165 799 268 198 133 1170 207 197
Cuts at: 4277 5801 6067 6115 6142 6653 6930 140
Size: 1524 266 48 27 511 277 3394
Fragments arranged by size:
3394 1524 1170 1165 799 511 277 268
266 207 198 197 133 48 27
Bpu10I CC'TnA_GC
Cuts at: 664 722 1057 2366 2788 4216 5927 5961 664
Size: 58 335 1309 422 1428 1711 34 4887
Fragments arranged by size:
4887 1711 1428 1309 422 335 58 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 7119 7360 8239 8731 9029 2500
Size: 4619 241 879 492 298 3655
Fragments arranged by size:
4619 3655 879 492 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 1831 2011 2101 4109 4787 4951 5485 6369 9888
Size: 180 90 2008 678 164 534 884 3519
Cuts at: 9888 1831
Size: 2127
Fragments arranged by size:
3519 2127 2008 884 678 534 180 164
90
BsaAI yAC'GTr
Cuts at: 1359 5180 5732 7009 7537 1359
Size: 3821 552 1277 528 4006
Fragments arranged by size:
4006 3821 1277 552 528
BsaBI GATnn'nnATC
Cuts at: 6 7698 9735 6
Size: 7692 2037 455
Fragments arranged by size:
7692 2037 455
BsaHI Gr'CG_yC
Cuts at: 3208 5258 6202 6692 7615 7863 8565 10061 3208
Size: 2050 944 490 923 248 702 1496 3331
Fragments arranged by size:
3331 2050 1496 944 923 702 490 248
BsaWI w'CCGG_w
Cuts at: 1624 1944 3642 3971 5155 7373 7894 8572 8846
Size: 320 1698 329 1184 2218 521 678 274
Cuts at: 8846 8993 9404 1624
Size: 147 411 2404
Fragments arranged by size:
2404 2218 1698 1184 678 521 411 329
320 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 275 305 1534 1564 2303 2333 4771 4801 6140
Size: 30 1229 30 739 30 2438 30 1339
Cuts at: 6140 6170 6626 6656 275
Size: 30 456 30 3803
Fragments arranged by size:
3803 2438 1339 1229 739 456 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1273 2518 2521 3446 3780 4597 4897 4900 5725
Size: 1245 3 925 334 817 300 3 825
Cuts at: 5725 6565 6933 6983 1273
Size: 840 368 50 4474
Fragments arranged by size:
4474 1245 925 840 825 817 368 334
300 50 3 3
BseYI C'CCAG_C
Cuts at: 145 348 699 820 921 1165 1399 1874 2268
Size: 203 351 121 101 244 234 475 394
Cuts at: 2268 2897 3278 3299 3769 4901 5687 6713 7069
Size: 629 381 21 470 1132 786 1026 356
Cuts at: 7069 7609 8367 8944 9440 145
Size: 540 758 577 496 889
Fragments arranged by size:
1132 1026 889 786 758 629 577 540
496 475 470 394 381 356 351 244
234 203 121 101 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 781 2061 2464 2999 3527 4162 5674 5900
Size: 230 1280 403 535 528 635 1512 226
Cuts at: 5900 6065 551
Size: 165 4670
Fragments arranged by size:
4670 1512 1280 635 535 528 403 230
226 165
BsiEI CG_ry'CG
Cuts at: 104 657 7726 7772 8980 104
Size: 553 7069 46 1208 1308
Fragments arranged by size:
7069 1308 1208 553 46
BsiHKAI G_wGCw'C
Cuts at: 465 1058 1088 1221 1444 1658 2121 2168 2958
Size: 593 30 133 223 214 463 47 790
Cuts at: 2958 4019 4163 5933 6681 7135 7198 7976 8166
Size: 1061 144 1770 748 454 63 778 190
Cuts at: 8166 8958 465
Size: 792 1691
Fragments arranged by size:
1770 1691 1061 792 790 778 748 593
463 454 223 214 190 144 133 63
47 30
BsiWI C'GTAC_G
Cuts at: 1144 1144
Size: 10184
BsmBI CGTCTCn'nnnn_
Cuts at: 198 620 941 5183 198
Size: 422 321 4242 5199
Fragments arranged by size:
5199 4242 422 321
Bsp1286I G_dGCh'C
Cuts at: 434 465 653 833 886 1058 1088 1188 1221
Size: 31 188 180 53 172 30 100 33
Cuts at: 1221 1444 1532 1580 1601 1632 1658 1894 2121
Size: 223 88 48 21 31 26 236 227
Cuts at: 2121 2168 2190 2252 2269 2730 2958 3087 3279
Size: 47 22 62 17 461 228 129 192
Cuts at: 3279 3761 4019 4163 5805 5883 5926 5933 5941
Size: 482 258 144 1642 78 43 7 8
Cuts at: 5941 6044 6101 6175 6639 6681 6896 7066 7135
Size: 103 57 74 464 42 215 170 69
Cuts at: 7135 7198 7809 7902 7976 8166 8228 8958 434
Size: 63 611 93 74 190 62 730 1660
Fragments arranged by size:
1660 1642 730 611 482 464 461 258
236 228 227 223 215 192 190 188
180 172 170 144 129 103 100 93
88 78 74 74 69 63 62 62
57 53 48 47 43 42 33 31
31 30 26 22 21 17 8 7
BspEI T'CCGG_A
Cuts at: 5155 9404 5155
Size: 4249 5935
BspHI T'CATG_A
Cuts at: 891 2664 5090 9360 891
Size: 1773 2426 4270 1715
Fragments arranged by size:
4270 2426 1773 1715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2718 3021 3225 4863 5503 5742 5753 7234 7448
Size: 303 204 1638 640 239 11 1481 214
Cuts at: 7448 7750 8131 2718
Size: 302 381 4771
Fragments arranged by size:
4771 1638 1481 640 381 303 302 239
214 204 11
BsrBI CCG'CTC
Cuts at: 50 3765 5760 6361 8476 8530 10014 10179 50
Size: 3715 1995 601 2115 54 1484 165 55
Fragments arranged by size:
3715 2115 1995 1484 601 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1410 4772 5024 8096 1410
Size: 3362 252 3072 3498
Fragments arranged by size:
3498 3362 3072 252
BsrFI r'CCGG_y
Cuts at: 255 1088 1944 3593 4139 4267 4524 6726 8182
Size: 833 856 1649 546 128 257 2202 1456
Cuts at: 8182 8363 8572 255
Size: 181 209 1867
Fragments arranged by size:
2202 1867 1649 1456 856 833 546 257
209 181 128
BsrGI T'GTAC_A
Cuts at: 3131 3131
Size: 10184
BssSI C'ACGA_G
Cuts at: 8455 8813 8455
Size: 358 9826
BstAPI GCAn_nnn'nTGC
Cuts at: 1376 1653 2953 7170 7561 1376
Size: 277 1300 4217 391 3999
Fragments arranged by size:
4217 3999 1300 391 277
BstBI TT'CG_AA
Cuts at: 1940 8545 1940
Size: 6605 3579
BstEII G'GTnAC_C
Cuts at: 693 693
Size: 10184
BstXI CCAn_nnnn'nTGG
Cuts at: 634 749 3920 5274 5736 634
Size: 115 3171 1354 462 5082
Fragments arranged by size:
5082 3171 1354 462 115
BstYI r'GATC_y
Cuts at: 1 264 603 3909 5398 7209 7676 7699 8034
Size: 263 339 3306 1489 1811 467 23 335
Cuts at: 8034 8280 9292 9378 9390 9736 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3306 1811 1489 1012 467 449 346 339
335 263 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3566 6953 3566
Size: 3387 6797
BtgI C'CryG_G
Cuts at: 1695 1860 2153 3523 4400 4757 5225 5282 1695
Size: 165 293 1370 877 357 468 57 6597
Fragments arranged by size:
6597 1370 877 468 357 293 165 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1240 2413 5577 8134 8315 8478 1240
Size: 1173 3164 2557 181 163 2946
Fragments arranged by size:
3164 2946 2557 1173 181 163
BtsI GCAGTG_nn'
Cuts at: 233 1642 2044 2814 4353 4871 5821 6028 7427
Size: 1409 402 770 1539 518 950 207 1399
Cuts at: 7427 233
Size: 2990
Fragments arranged by size:
2990 1539 1409 1399 950 770 518 402
207
ClaI AT'CG_AT
Cuts at: 1805 6496 1805
Size: 4691 5493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 320 355 320
Size: 35 10149
DraI TTT'AAA
Cuts at: 6536 7183 6536
Size: 647 9537
DraIII CAC_nnn'GTG
Cuts at: 4260 6124 7170 4260
Size: 1864 1046 7274
Fragments arranged by size:
7274 1864 1046
DrdI GACnn_nn'nnGTC
Cuts at: 132 691 7890 8748 132
Size: 559 7199 858 1568
Fragments arranged by size:
7199 1568 858 559
EaeI y'GGCC_r
Cuts at: 983 3495 3526 3575 3799 4196 4610 4989 5561
Size: 2512 31 49 224 397 414 379 572
Cuts at: 5561 5720 5993 6869 7152 7769 7943 8334 8361
Size: 159 273 876 283 617 174 391 27
Cuts at: 8361 9706 983
Size: 1345 1461
Fragments arranged by size:
2512 1461 1345 876 617 572 414 397
391 379 283 273 224 174 159 49
31 27
EagI C'GGCC_G
Cuts at: 7769 7769
Size: 10184
EarI CTCTTCn'nnn_
Cuts at: 939 2218 2387 2963 4124 4874 5593 5683 5702
Size: 1279 169 576 1161 750 719 90 19
Cuts at: 5702 6542 6654 8207 8417 939
Size: 840 112 1553 210 2706
Fragments arranged by size:
2706 1553 1279 1161 840 750 719 576
210 169 112 90 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1331 2017 5563 8392 8702 8848 9994 1331
Size: 686 3546 2829 310 146 1146 1521
Fragments arranged by size:
3546 2829 1521 1146 686 310 146
EcoICRI GAG'CTC
Cuts at: 1056 1219 6679 7133 7196 1056
Size: 163 5460 454 63 4044
Fragments arranged by size:
5460 4044 454 163 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 482 578 827 1196 1305 1766 2104 2216
Size: 342 96 249 369 109 461 338 112
Cuts at: 2216 2372 2500 2570 2703 3845 3873 4080 4277
Size: 156 128 70 133 1142 28 207 197
Cuts at: 4277 4496 5801 6067 6115 6142 6653 6930 8009
Size: 219 1305 266 48 27 511 277 1079
Cuts at: 8009 8441 9188 9456 140
Size: 432 747 268 868
Fragments arranged by size:
1305 1142 1079 868 747 511 461 432
369 342 338 277 268 266 249 219
207 197 156 133 128 112 109 96
70 48 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 877 1331 1378 3102 4514 5749 6945 7055 877
Size: 454 47 1724 1412 1235 1196 110 4006
Fragments arranged by size:
4006 1724 1412 1235 1196 454 110 47
EcoO109I rG'GnC_Cy
Cuts at: 421 811 882 883 1383 1396 1577 1721 2240
Size: 390 71 1 500 13 181 144 519
Cuts at: 2240 2248 2511 3570 3707 3954 4281 4603 5307
Size: 8 263 1059 137 247 327 322 704
Cuts at: 5307 6040 6041 6055 6076 6297 6328 6625 6710
Size: 733 1 14 21 221 31 297 85
Cuts at: 6710 6847 6864 7062 7063 7323 421
Size: 137 17 198 1 260 3282
Fragments arranged by size:
3282 1059 733 704 519 500 390 327
322 297 263 260 247 221 198 181
144 137 137 85 71 31 21 17
14 13 8 1 1 1
EcoRI G'AATT_C
Cuts at: 7188 7188
Size: 10184
EcoRV GAT'ATC
Cuts at: 3820 3820
Size: 10184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3125 4442 5406 8203 3125
Size: 1317 964 2797 5106
Fragments arranged by size:
5106 2797 1317 964
FspI TGC'GCA
Cuts at: 7965 7965
Size: 10184
HaeII r_GCGC'y
Cuts at: 292 2347 3211 6205 7618 7866 8888 9867 292
Size: 2055 864 2994 1413 248 1022 979 609
Fragments arranged by size:
2994 2055 1413 1022 979 864 609 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 275 307 1303 1335 1534 1566 1654 1686 2303
Size: 32 996 32 199 32 88 32 617
Cuts at: 2303 2335 2386 2418 2699 2731 4053 4085 4488
Size: 32 51 32 281 32 1322 32 403
Cuts at: 4488 4520 4687 4719 4873 4905 5248 5280 6140
Size: 32 167 32 154 32 343 32 860
Cuts at: 6140 6172 8026 8056 8058 8088 275
Size: 32 1854 30 2 30 2371
Fragments arranged by size:
2371 1854 1322 996 860 617 403 343
281 199 167 154 88 51 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3426 6690 7223 3426
Size: 3264 533 6387
Fragments arranged by size:
6387 3264 533
HindIII A'AGCT_T
Cuts at: 1778 5383 6467 1778
Size: 3605 1084 5495
Fragments arranged by size:
5495 3605 1084
KasI G'GCGC_C
Cuts at: 3207 6201 7614 7862 3207
Size: 2994 1413 248 5529
Fragments arranged by size:
5529 2994 1413 248
KpnI G_GTAC'C
Cuts at: 7204 7204
Size: 10184
MluI A'CGCG_T
Cuts at: 9617 9617
Size: 10184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 259 1740 2797 5261 5831 6189 6601 8855 9039
Size: 1481 1057 2464 570 358 412 2254 184
Cuts at: 9039 259
Size: 1404
Fragments arranged by size:
2464 2254 1481 1404 1057 570 412 358
184
MscI TGG'CCA
Cuts at: 985 3528 4198 4612 5563 5995 6871 7154 7945
Size: 2543 670 414 951 432 876 283 791
Cuts at: 7945 985
Size: 3224
Fragments arranged by size:
3224 2543 951 876 791 670 432 414
283
MslI CAynn'nnrTG
Cuts at: 398 632 686 1447 2995 3028 3938 4457 5582
Size: 234 54 761 1548 33 910 519 1125
Cuts at: 5582 5730 5795 8173 8300 398
Size: 148 65 2378 127 2282
Fragments arranged by size:
2378 2282 1548 1125 910 761 519 234
148 127 65 54 33
MspA1I CmG'CkG
Cuts at: 824 1463 1594 2050 3441 3505 5691 6717 7073
Size: 639 131 456 1391 64 2186 1026 356
Cuts at: 7073 7104 7295 7609 7969 8982 9227 824
Size: 31 191 314 360 1013 245 1781
Fragments arranged by size:
2186 1781 1391 1026 1013 639 456 360
356 314 245 191 131 64 31
NaeI GCC'GGC
Cuts at: 4526 6728 8365 4526
Size: 2202 1637 6345
Fragments arranged by size:
6345 2202 1637
NarI GG'CG_CC
Cuts at: 3208 6202 7615 7863 3208
Size: 2994 1413 248 5529
Fragments arranged by size:
5529 2994 1413 248
NcoI C'CATG_G
Cuts at: 4400 5225 5282 4400
Size: 825 57 9302
Fragments arranged by size:
9302 825 57
NgoMIV G'CCGG_C
Cuts at: 4524 6726 8363 4524
Size: 2202 1637 6345
Fragments arranged by size:
6345 2202 1637
NheI G'CTAG_C
Cuts at: 187 187
Size: 10184
NruI TCG'CGA
Cuts at: 5596 5596
Size: 10184
NspI r_CATG'y
Cuts at: 403 469 645 2172 6651 7237 7737 8644 403
Size: 66 176 1527 4479 586 500 907 1943
Fragments arranged by size:
4479 1943 1527 907 586 500 176 66
PasI CC'CwG_GG
Cuts at: 1327 2244 3252 5481 5883 6073 6181 6304 6622
Size: 917 1008 2229 402 190 108 123 318
Cuts at: 6622 6963 1327
Size: 341 4548
Fragments arranged by size:
4548 2229 1008 917 402 341 318 190
123 108
PciI A'CATG_T
Cuts at: 6647 8640 6647
Size: 1993 8191
PflMI CCAn_nnn'nTGG
Cuts at: 1158 1731 2308 4260 4369 4548 4632 5481 1158
Size: 573 577 1952 109 179 84 849 5861
Fragments arranged by size:
5861 1952 849 577 573 179 109 84
PfoI T'CCnGG_A
Cuts at: 594 1068 2680 2961 5266 6092 6158 6630 7126
Size: 474 1612 281 2305 826 66 472 496
Cuts at: 7126 594
Size: 3652
Fragments arranged by size:
3652 2305 1612 826 496 474 472 281
66
PmeI GTTT'AAAC
Cuts at: 7183 7183
Size: 10184
PmlI CAC'GTG
Cuts at: 1359 5180 5732 7009 1359
Size: 3821 552 1277 4534
Fragments arranged by size:
4534 3821 1277 552
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2445 6637 6663 6669 6695 9323 9355 2413
Size: 32 4192 26 6 26 2628 32 3242
Fragments arranged by size:
4192 3242 2628 32 32 26 26 6
PpuMI rG'GwC_Cy
Cuts at: 421 811 2240 3570 3954 4603 6055 6328 6710
Size: 390 1429 1330 384 649 1452 273 382
Cuts at: 6710 421
Size: 3895
Fragments arranged by size:
3895 1452 1429 1330 649 390 384 382
273
PshAI GACnn'nnGTC
Cuts at: 2023 3431 6326 2023
Size: 1408 2895 5881
Fragments arranged by size:
5881 2895 1408
PspOMI G'GGCC_C
Cuts at: 882 1576 2248 6040 7062 882
Size: 694 672 3792 1022 4004
Fragments arranged by size:
4004 3792 1022 694 672
PspXI vC'TCGA_Gb
Cuts at: 5934 6273 5934
Size: 339 9845
PstI C_TGCA'G
Cuts at: 737 2337 2579 2715 4565 5135 5750 6140 6855
Size: 1600 242 136 1850 570 615 390 715
Cuts at: 6855 6915 7059 7231 7916 737
Size: 60 144 172 685 3005
Fragments arranged by size:
3005 1850 1600 715 685 615 570 390
242 172 144 136 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10184
PvuII CAG'CTG
Cuts at: 1594 2050 3441 3505 5691 7104 7609 7969 1594
Size: 456 1391 64 2186 1413 505 360 3809
Fragments arranged by size:
3809 2186 1413 1391 505 456 360 64
RsrII CG'GwC_CG
Cuts at: 8379 8379
Size: 10184
SacI G_AGCT'C
Cuts at: 1058 1221 6681 7135 7198 1058
Size: 163 5460 454 63 4044
Fragments arranged by size:
5460 4044 454 163 63
SalI G'TCGA_C
Cuts at: 3424 7221 3424
Size: 3797 6387
SanDI GG'GwC_CC
Cuts at: 421 3954 4603 6328 6710 421
Size: 3533 649 1725 382 3895
Fragments arranged by size:
3895 3533 1725 649 382
SapI GCTCTTCn'nnn_
Cuts at: 2963 5593 6654 8207 8417 2963
Size: 2630 1061 1553 210 4730
Fragments arranged by size:
4730 2630 1553 1061 210
SbfI CC_TGCA'GG
Cuts at: 7231 7231
Size: 10184
ScaI AGT'ACT
Cuts at: 3625 9701 3625
Size: 6076 4108
SexAI A'CCwGG_T
Cuts at: 5095 6120 7166 5095
Size: 1025 1046 8113
Fragments arranged by size:
8113 1046 1025
SfcI C'TryA_G
Cuts at: 33 733 1680 1904 2333 2575 2711 3681 3834
Size: 700 947 224 429 242 136 970 153
Cuts at: 3834 3936 4561 5131 5746 6136 6851 6911 6984
Size: 102 625 570 615 390 715 60 73
Cuts at: 6984 7055 7227 7912 8905 9096 33
Size: 71 172 685 993 191 1121
Fragments arranged by size:
1121 993 970 947 715 700 685 625
615 570 429 390 242 224 191 172
153 136 102 73 71 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 3263 3263
Size: 10184
SfoI GGC'GCC
Cuts at: 3209 6203 7616 7864 3209
Size: 2994 1413 248 5529
Fragments arranged by size:
5529 2994 1413 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10184
SmaI CCC'GGG
Cuts at: 1123 4601 6038 7206 1123
Size: 3478 1437 1168 4101
Fragments arranged by size:
4101 3478 1437 1168
SmlI C'TyrA_G
Cuts at: 2479 3099 4948 5934 6273 7134 7339 8254 8746
Size: 620 1849 986 339 861 205 915 492
Cuts at: 8746 9008 2479
Size: 262 3655
Fragments arranged by size:
3655 1849 986 915 861 620 492 339
262 205
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10184
SphI G_CATG'C
Cuts at: 7237 7737 7237
Size: 500 9684
SspI AAT'ATT
Cuts at: 1913 1913
Size: 10184
StuI AGG'CCT
Cuts at: 2229 4555 6951 7114 2229
Size: 2326 2396 163 5299
Fragments arranged by size:
5299 2396 2326 163
StyI C'CwwG_G
Cuts at: 1236 2195 4400 4529 5225 5282 5717 6896 7318
Size: 959 2205 129 696 57 435 1179 422
Cuts at: 7318 7672 1236
Size: 354 3748
Fragments arranged by size:
3748 2205 1179 959 696 435 422 354
129 57
SwaI ATTT'AAAT
Cuts at: 6536 6536
Size: 10184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 671 1169 4639 4783 4975 5473 5742 6516
Size: 142 498 3470 144 192 498 269 774
Cuts at: 6516 7767 7900 8359 8568 529
Size: 1251 133 459 209 2145
Fragments arranged by size:
3470 2145 1251 774 498 498 459 269
209 192 144 142 133
TatI w'GTAC_w
Cuts at: 298 1006 3131 3623 6650 9699 298
Size: 708 2125 492 3027 3049 783
Fragments arranged by size:
3049 3027 2125 783 708 492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 831 863 4908 4940 5611 5643 6074 6106 6626
Size: 32 4045 32 671 32 431 32 520
Cuts at: 6626 6658 9701 9733 831
Size: 32 3043 32 1282
Fragments arranged by size:
4045 3043 1282 671 520 431 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4387 5867 6748 7981 4387
Size: 1480 881 1233 6590
Fragments arranged by size:
6590 1480 1233 881
XbaI T'CTAG_A
Cuts at: 6655 7215 6655
Size: 560 9624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 967 1570 1732 1858 2530 3259 3901 5353 5901
Size: 603 162 126 672 729 642 1452 548
Cuts at: 5901 7679 967
Size: 1778 3472
Fragments arranged by size:
3472 1778 1452 729 672 642 603 548
162 126
XhoI C'TCGA_G
Cuts at: 4948 5934 6273 4948
Size: 986 339 8859
Fragments arranged by size:
8859 986 339
XmaI C'CCGG_G
Cuts at: 1121 4599 6036 7204 1121
Size: 3478 1437 1168 4101
Fragments arranged by size:
4101 3478 1437 1168
XmnI GAAnn'nnTTC
Cuts at: 1499 1499
Size: 10184
ZraI GAC'GTC
Cuts at: 5259 6693 5259
Size: 1434 8750
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsmI BssHII BstZ17I
FseI FspAI HpaI MfeI NdeI NotI NsiI PacI
PsiI PsrI SacII SgrAI SnaBI SrfI