Restriction map for
pF1KA0309
(Circular) (Six-base) MAPSORT of: pF1KA0309.seq Check: 4239 from: 1 to: 12260
pF1KA0309 12260 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3210 7719 8133 3210
Size: 4509 414 7337
Fragments arranged by size:
7337 4509 414
AatII G_ACGT'C
Cuts at: 9135 9135
Size: 12260
AccI GT'mk_AC
Cuts at: 4471 9298 4471
Size: 4827 7433
Acc65I G'GTAC_C
Cuts at: 4506 4737 9276 4506
Size: 231 4539 7490
Fragments arranged by size:
7490 4539 231
AclI AA'CG_TT
Cuts at: 2100 12100 2100
Size: 10000 2260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1154 1166 1643 2790 3710 3734 4898 5345
Size: 733 12 477 1147 920 24 1164 447
Cuts at: 5345 6263 6893 7496 7540 7664 9107 10085 10517
Size: 918 630 603 44 124 1443 978 432
Cuts at: 10517 11264 11532 421
Size: 747 268 1149
Fragments arranged by size:
1443 1164 1149 1147 978 920 918 747
733 630 603 477 447 432 268 124
44 24 12
AfeI AGC'GCT
Cuts at: 2311 6505 2311
Size: 4194 8066
AflII C'TTAA_G
Cuts at: 5667 5667
Size: 12260
AflIII A'CryG_T
Cuts at: 4161 9612 10716 11693 4161
Size: 5451 1104 977 4728
Fragments arranged by size:
5451 4728 1104 977
AgeI A'CCGG_T
Cuts at: 2769 4503 10648 2769
Size: 1734 6145 4381
Fragments arranged by size:
6145 4381 1734
AhdI GACnn_n'nnGTC
Cuts at: 777 4127 5469 777
Size: 3350 1342 7568
Fragments arranged by size:
7568 3350 1342
AleI CACnn'nnGTG
Cuts at: 2068 3673 7894 2068
Size: 1605 4221 6434
Fragments arranged by size:
6434 4221 1605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1406 6485 7912 1406
Size: 5079 1427 5754
Fragments arranged by size:
5754 5079 1427
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5261 5293 6607 6639 5261
Size: 32 1314 32 10882
Fragments arranged by size:
10882 1314 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1414 1534 1810 3127 4417 4605 4758 4800 4926
Size: 120 276 1317 1290 188 153 42 126
Cuts at: 4926 5959 5981 6952 7330 7444 7543 8182 8251
Size: 1033 22 971 378 114 99 639 69
Cuts at: 8251 8281 9565 11132 1414
Size: 30 1284 1567 2542
Fragments arranged by size:
2542 1567 1317 1290 1284 1033 971 639
378 276 188 153 126 120 114 99
69 42 30 22
ApaI G_GGCC'C
Cuts at: 298 588 1484 1763 3100 3212 3973 4849 4963
Size: 290 896 279 1337 112 761 876 114
Cuts at: 4963 5304 7988 8312 8994 298
Size: 341 2684 324 682 3564
Fragments arranged by size:
3564 2684 1337 896 876 761 682 341
324 290 279 114 112
ApaLI G'TGCA_C
Cuts at: 11030 11030
Size: 12260
ApoI r'AATT_y
Cuts at: 6373 6517 9264 11613 11869 6373
Size: 144 2747 2349 256 6764
Fragments arranged by size:
6764 2747 2349 256 144
AseI AT'TA_AT
Cuts at: 20 20
Size: 12260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12260
AvaI C'yCGr_G
Cuts at: 1121 1349 3515 3647 4581 5612 6038 6452 6770
Size: 228 2166 132 934 1031 426 414 318
Cuts at: 6770 7926 8086 8662 9280 1121
Size: 1156 160 576 618 4101
Fragments arranged by size:
4101 2166 1156 1031 934 618 576 426
414 318 228 160 132
AvrII C'CTAG_G
Cuts at: 6886 6886
Size: 12260
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5060 5093 5060
Size: 33 12227
BamHI G'GATC_C
Cuts at: 9285 9285
Size: 12260
BanI G'GyrC_C
Cuts at: 235 417 551 2105 2936 3087 3409 4005 4044
Size: 182 134 1554 831 151 322 596 39
Cuts at: 4044 4212 4401 4506 4737 4767 5050 5307 5517
Size: 168 189 105 231 30 283 257 210
Cuts at: 5517 6694 7434 8202 9276 9690 9938 9973 235
Size: 1177 740 768 1074 414 248 35 2522
Fragments arranged by size:
2522 1554 1177 1074 831 768 740 596
414 322 283 257 248 231 210 189
182 168 151 134 105 39 35 30
BanII G_rGCy'C
Cuts at: 298 428 588 1157 1484 1763 2142 3100 3134
Size: 130 160 569 327 279 379 958 34
Cuts at: 3134 3212 3571 3654 3973 4588 4849 4939 4963
Size: 78 359 83 319 615 261 90 24
Cuts at: 4963 5304 5606 5619 6560 6771 7526 7988 8312
Size: 341 302 13 941 211 755 462 324
Cuts at: 8312 8994 9274 10304 298
Size: 682 280 1030 2254
Fragments arranged by size:
2254 1030 958 941 755 682 615 569
462 379 359 341 327 324 319 302
280 279 261 211 160 130 90 83
78 34 24 13
BbeI G_GCGC'C
Cuts at: 3091 9694 9942 3091
Size: 6603 248 5409
Fragments arranged by size:
6603 5409 248
BbsI GAAGACnn'nnnn_
Cuts at: 1990 2418 4103 6751 1990
Size: 428 1685 2648 7499
Fragments arranged by size:
7499 2648 1685 428
BbvCI CC'TCA_GC
Cuts at: 846 1061 1295 4494 6434 7448 7533 7652 846
Size: 215 234 3199 1940 1014 85 119 5454
Fragments arranged by size:
5454 3199 1940 1014 234 215 119 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 8910 8944 8910
Size: 34 12226
BciVI GTATCCnnnnn_n'
Cuts at: 1275 5088 10158 10919 12260 1275
Size: 3813 5070 761 1341 1275
Fragments arranged by size:
5070 3813 1341 1275 761
BclI T'GATC_A
Cuts at: 9780 9780
Size: 12260
BglI GCCn_nnn'nGGC
Cuts at: 2017 3328 5372 5434 6340 7821 2017
Size: 1311 2044 62 906 1481 6456
Fragments arranged by size:
6456 2044 1481 1311 906 62
BglII A'GATC_T
Cuts at: 7314 9775 11812 7314
Size: 2461 2037 7762
Fragments arranged by size:
7762 2461 2037
BlpI GC'TnA_GC
Cuts at: 371 1336 6362 6458 9372 371
Size: 965 5026 96 2914 3259
Fragments arranged by size:
5026 3259 2914 965 96
Bme1580I G_kGCm'C
Cuts at: 248 298 588 1484 1763 2110 3100 3212 3414
Size: 50 290 896 279 347 990 112 202
Cuts at: 3414 3973 4849 4963 5304 6233 7169 7230 7307
Size: 559 876 114 341 929 936 61 77
Cuts at: 7307 7988 8312 8994 9885 9978 11034 248
Size: 681 324 682 891 93 1056 1474
Fragments arranged by size:
1474 1056 990 936 929 896 891 876
682 681 559 347 341 324 290 279
202 114 112 93 77 61 50
BmgBI CAC'GTC
Cuts at: 5569 5569
Size: 12260
BmrI ACTGGGnnnn_n'
Cuts at: 456 2046 2091 3100 3151 4792 4939 5649 6940
Size: 1590 45 1009 51 1641 147 710 1291
Cuts at: 6940 6952 8239 8989 9472 9553 9888 12032 456
Size: 12 1287 750 483 81 335 2144 684
Fragments arranged by size:
2144 1641 1590 1291 1287 1009 750 710
684 483 335 147 81 51 45 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2475 6574 8125 8223 10627 2475
Size: 4099 1551 98 2404 4108
Fragments arranged by size:
4108 4099 2404 1551 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 271 675 792 840 1739 1804 2836 3104
Size: 68 404 117 48 899 65 1032 268
Cuts at: 3104 4247 4271 4352 4460 4706 4730 4796 4814
Size: 1143 24 81 108 246 24 66 18
Cuts at: 4814 4823 4862 5251 6226 6307 6544 6556 6887
Size: 9 39 389 975 81 237 12 331
Cuts at: 6887 7091 7229 7276 7540 7588 7602 8156 203
Size: 204 138 47 264 48 14 554 4307
Fragments arranged by size:
4307 1143 1032 975 899 554 404 389
331 268 264 246 237 204 138 117
108 81 81 68 66 65 48 48
47 39 24 24 18 14 12 9
Bpu10I CC'TnA_GC
Cuts at: 494 846 1061 1295 2827 3044 3128 4268 4494
Size: 352 215 234 1532 217 84 1140 226
Cuts at: 4494 6002 6434 7448 7533 7652 494
Size: 1508 432 1014 85 119 5102
Fragments arranged by size:
5102 1532 1508 1140 1014 432 352 234
226 217 215 119 85 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 721 2143 3673 4953 5713 6767 7224 9436
Size: 542 1422 1530 1280 760 1054 457 2212
Cuts at: 9436 10315 10807 11105 179
Size: 879 492 298 1334
Fragments arranged by size:
2212 1530 1422 1334 1280 1054 879 760
542 492 457 298
BsaI GGTCTCn'nnnn_
Cuts at: 7911 8174 8254 8901 8945 11964 7911
Size: 263 80 647 44 3019 8207
Fragments arranged by size:
8207 3019 647 263 80 44
BsaAI yAC'GTr
Cuts at: 3771 9613 3771
Size: 5842 6418
BsaBI GATnn'nnATC
Cuts at: 6 2843 9774 11811 6
Size: 2837 6931 2037 455
Fragments arranged by size:
6931 2837 2037 455
BsaHI Gr'CG_yC
Cuts at: 1861 2346 3088 5184 7728 9132 9691 9939 10641
Size: 485 742 2096 2544 1404 559 248 702
Cuts at: 10641 12137 1861
Size: 1496 1984
Fragments arranged by size:
2544 2096 1984 1496 1404 742 702 559
485 248
BsaWI w'CCGG_w
Cuts at: 1544 2769 4392 4446 4503 9449 9970 10648 10922
Size: 1225 1623 54 57 4946 521 678 274
Cuts at: 10922 11069 11480 1544
Size: 147 411 2324
Fragments arranged by size:
4946 2324 1623 1225 678 521 411 274
147 57 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2077 2107 2336 2349 2366 2379 2380 2410 4860
Size: 30 229 13 17 13 1 30 2450
Cuts at: 4860 4890 5913 5943 6607 6637 7053 7083 7089
Size: 30 1023 30 664 30 416 30 6
Cuts at: 7089 7119 2077
Size: 30 7218
Fragments arranged by size:
7218 2450 1023 664 416 229 30 30
30 30 30 30 30 17 13 13
6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 574 610 1387 1493 1591 1681 2314 3107 3752
Size: 36 777 106 98 90 633 793 645
Cuts at: 3752 3813 4364 4367 4376 6097 6262 6457 6553
Size: 61 551 3 9 1721 165 195 96
Cuts at: 6553 6568 6631 6655 6658 6661 7007 7058 7130
Size: 15 63 24 3 3 346 51 72
Cuts at: 7130 7278 7498 7712 9088 9115 9118 574
Size: 148 220 214 1376 27 3 3716
Fragments arranged by size:
3716 1721 1376 793 777 645 633 551
346 220 214 195 165 148 106 98
96 90 72 63 61 51 36 27
24 15 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 150 2110 3140 3549 3912 3972 4017 4191 4617
Size: 1960 1030 409 363 60 45 174 426
Cuts at: 4617 4848 4854 4908 4992 5010 5137 5392 5450
Size: 231 6 54 84 18 127 255 58
Cuts at: 5450 6661 7384 8027 8061 8182 8312 8327 8620
Size: 1211 723 643 34 121 130 15 293
Cuts at: 8620 9685 10443 11020 11516 150
Size: 1065 758 577 496 894
Fragments arranged by size:
1960 1211 1065 1030 894 758 723 643
577 496 426 409 363 293 255 231
174 130 127 121 84 60 58 54
45 34 18 15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4939 6350 7044 7364 4939
Size: 1411 694 320 9835
Fragments arranged by size:
9835 1411 694 320
BsiEI CG_ry'CG
Cuts at: 104 2770 4967 9802 9848 11056 104
Size: 2666 2197 4835 46 1208 1308
Fragments arranged by size:
4835 2666 2197 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3134 3571 5606 5753 6560 7526 8106 9274 10052
Size: 437 2035 147 807 966 580 1168 778
Cuts at: 10052 10242 11034 3134
Size: 190 792 4360
Fragments arranged by size:
4360 2035 1168 966 807 792 778 580
437 190 147
BsmI GAATG_Cn'
Cuts at: 1955 5780 8795 1955
Size: 3825 3015 5420
Fragments arranged by size:
5420 3825 3015
BsmBI CGTCTCn'nnnn_
Cuts at: 2337 8090 2337
Size: 5753 6507
Bsp1286I G_dGCh'C
Cuts at: 248 298 428 588 1157 1484 1763 2110 2142
Size: 50 130 160 569 327 279 347 32
Cuts at: 2142 3100 3134 3212 3414 3571 3654 3973 4588
Size: 958 34 78 202 157 83 319 615
Cuts at: 4588 4849 4939 4963 5304 5606 5619 5753 6233
Size: 261 90 24 341 302 13 134 480
Cuts at: 6233 6560 6771 7169 7230 7307 7526 7988 8106
Size: 327 211 398 61 77 219 462 118
Cuts at: 8106 8312 8994 9274 9885 9978 10052 10242 10304
Size: 206 682 280 611 93 74 190 62
Cuts at: 10304 11034 248
Size: 730 1474
Fragments arranged by size:
1474 958 730 682 615 611 569 480
462 398 347 341 327 327 319 302
280 279 261 219 211 206 202 190
160 157 134 130 118 93 90 83
78 77 74 62 61 50 34 32
24 13
BspEI T'CCGG_A
Cuts at: 11480 11480
Size: 12260
BspHI T'CATG_A
Cuts at: 1107 2699 11436 1107
Size: 1592 8737 1931
Fragments arranged by size:
8737 1931 1592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 737 3210 5831 7719 8133 9310 9524 9826 10207
Size: 2473 2621 1888 414 1177 214 302 381
Cuts at: 10207 737
Size: 2790
Fragments arranged by size:
2790 2621 2473 1888 1177 414 381 302
214
BsrBI CCG'CTC
Cuts at: 50 5056 10552 10606 12090 12255 50
Size: 5006 5496 54 1484 165 55
Fragments arranged by size:
5496 5006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 666 978 1796 5119 7518 8039 10172 666
Size: 312 818 3323 2399 521 2133 2754
Fragments arranged by size:
3323 2754 2399 2133 818 521 312
BsrFI r'CCGG_y
Cuts at: 2769 3617 4503 6422 6701 9146 10258 10439 10648
Size: 848 886 1919 279 2445 1112 181 209
Cuts at: 10648 2769
Size: 4381
Fragments arranged by size:
4381 2445 1919 1112 886 848 279 209
181
BssSI C'ACGA_G
Cuts at: 556 6743 8399 10531 10889 556
Size: 6187 1656 2132 358 1927
Fragments arranged by size:
6187 2132 1927 1656 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4297 5050 5900 6928 9637 4297
Size: 753 850 1028 2709 6920
Fragments arranged by size:
6920 2709 1028 850 753
BstBI TT'CG_AA
Cuts at: 7806 10621 7806
Size: 2815 9445
BstEII G'GTnAC_C
Cuts at: 1926 2772 4129 1926
Size: 846 1357 10057
Fragments arranged by size:
10057 1357 846
BstXI CCAn_nnnn'nTGG
Cuts at: 1909 4323 5555 8320 8823 1909
Size: 2414 1232 2765 503 5346
Fragments arranged by size:
5346 2765 2414 1232 503
BstYI r'GATC_y
Cuts at: 1 5836 7314 7337 8277 9285 9752 9775 10110
Size: 5835 1478 23 940 1008 467 23 335
Cuts at: 10110 10356 11368 11454 11466 11812 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
5835 1478 1012 1008 940 467 449 346
335 246 86 23 23 12
Bsu36I CC'TnA_GG
Cuts at: 413 1160 1505 7211 7697 9059 9093 413
Size: 747 345 5706 486 1362 34 3580
Fragments arranged by size:
5706 3580 1362 747 486 345 34
BtgI C'CryG_G
Cuts at: 629 1328 1367 1484 2834 3082 3467 4184 5072
Size: 699 39 117 1350 248 385 717 888
Cuts at: 5072 5808 5993 8816 629
Size: 736 185 2823 4073
Fragments arranged by size:
4073 2823 1350 888 736 717 699 385
248 185 117 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2422 4391 10210 10391 10554 2422
Size: 1969 5819 181 163 4128
Fragments arranged by size:
5819 4128 1969 181 163
BtsI GCAGTG_nn'
Cuts at: 478 1428 1746 3259 5150 5717 8011 8104 8503
Size: 950 318 1513 1891 567 2294 93 399
Cuts at: 8503 8519 9503 478
Size: 16 984 3235
Fragments arranged by size:
3235 2294 1891 1513 984 950 567 399
318 93 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2002 2037 2002
Size: 35 12225
DraI TTT'AAA
Cuts at: 2118 9259 2118
Size: 7141 5119
DraIII CAC_nnn'GTG
Cuts at: 5748 5748
Size: 12260
DrdI GACnn_nn'nnGTC
Cuts at: 9966 10824 9966
Size: 858 11402
EaeI y'GGCC_r
Cuts at: 632 1841 3615 4091 4229 4964 5811 6030 6131
Size: 1209 1774 476 138 735 847 219 101
Cuts at: 6131 6263 6420 7271 7774 9845 10019 10410 10437
Size: 132 157 851 503 2071 174 391 27
Cuts at: 10437 11782 632
Size: 1345 1110
Fragments arranged by size:
2071 1774 1345 1209 1110 851 847 735
503 476 391 219 174 157 138 132
101 27
EagI C'GGCC_G
Cuts at: 4964 9845 4964
Size: 4881 7379
EarI CTCTTCn'nnn_
Cuts at: 304 397 458 828 846 920 1031 1145 1235
Size: 93 61 370 18 74 111 114 90
Cuts at: 1235 1253 1271 1490 1619 1658 1709 3689 3762
Size: 18 18 219 129 39 51 1980 73
Cuts at: 3762 3804 4161 4394 6106 6239 6248 6311 6566
Size: 42 357 233 1712 133 9 63 255
Cuts at: 6566 7583 8575 8797 10283 10493 304
Size: 1017 992 222 1486 210 2071
Fragments arranged by size:
2071 1980 1712 1486 1017 992 370 357
255 233 222 219 210 133 129 114
111 93 90 74 73 63 61 51
42 39 18 18 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6616 6801 10468 10778 10924 12070 6616
Size: 185 3667 310 146 1146 6806
Fragments arranged by size:
6806 3667 1146 310 185 146
EcoICRI GAG'CTC
Cuts at: 3132 3569 5604 6558 7524 9272 3132
Size: 437 2035 954 966 1748 6120
Fragments arranged by size:
6120 2035 1748 966 954 437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 271 421 675 792 840 1154 1166 1643
Size: 68 150 254 117 48 314 12 477
Cuts at: 1643 1739 1804 2790 2836 3104 3710 3734 4247
Size: 96 65 986 46 268 606 24 513
Cuts at: 4247 4271 4352 4460 4706 4730 4796 4814 4823
Size: 24 81 108 246 24 66 18 9
Cuts at: 4823 4862 4898 5251 5345 6226 6263 6307 6544
Size: 39 36 353 94 881 37 44 237
Cuts at: 6544 6556 6887 6893 7091 7229 7276 7496 7540
Size: 12 331 6 198 138 47 220 44
Cuts at: 7540 7588 7602 7664 8156 9107 10085 10517 11264
Size: 48 14 62 492 951 978 432 747
Cuts at: 11264 11532 203
Size: 268 931
Fragments arranged by size:
986 978 951 931 881 747 606 513
492 477 432 353 331 314 268 268
254 246 237 220 198 150 138 117
108 96 94 81 68 66 65 62
48 48 47 46 44 44 39 37
36 24 24 24 18 14 12 12
9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1136 1754 2527 3119 6097 1136
Size: 618 773 592 2978 7299
Fragments arranged by size:
7299 2978 773 618 592
EcoO109I rG'GnC_Cy
Cuts at: 223 294 1075 1480 1759 2041 3097 3124 3209
Size: 71 781 405 279 282 1056 27 85
Cuts at: 3209 4690 5300 5301 7580 7702 7792 7985 8150
Size: 1481 610 1 2279 122 90 193 165
Cuts at: 8150 8309 8990 8991 9055 9119 9399 223
Size: 159 681 1 64 64 280 3084
Fragments arranged by size:
3084 2279 1481 1056 781 681 610 405
282 280 279 193 165 159 122 90
85 71 64 64 27 1 1
EcoRI G'AATT_C
Cuts at: 9264 9264
Size: 12260
EcoRV GAT'ATC
Cuts at: 6324 6324
Size: 12260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1378 10279 1378
Size: 8901 3359
FseI GG_CCGG'CC
Cuts at: 6426 6426
Size: 12260
FspI TGC'GCA
Cuts at: 538 5650 9190 10041 538
Size: 5112 3540 851 2757
Fragments arranged by size:
5112 3540 2757 851
FspAI rTGC'GCAy
Cuts at: 538 5650 538
Size: 5112 7148
HaeII r_GCGC'y
Cuts at: 504 2262 2313 3091 4987 6507 9200 9694 9942
Size: 1758 51 778 1896 1520 2693 494 248
Cuts at: 9942 10964 11943 504
Size: 1022 979 821
Fragments arranged by size:
2693 1896 1758 1520 1022 979 821 778
494 248 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 345 377 763 795 1627 1659 1986 2018 2336
Size: 32 386 32 832 32 327 32 318
Cuts at: 2336 2349 2368 2380 2381 2412 4113 4145 4788
Size: 13 19 12 1 31 1701 32 643
Cuts at: 4788 4820 4860 4892 5455 5487 5738 5770 6433
Size: 32 40 32 563 32 251 32 663
Cuts at: 6433 6465 7567 7599 8431 8463 10102 10132 10134
Size: 32 1102 32 832 32 1639 30 2
Cuts at: 10134 10164 345
Size: 30 2441
Fragments arranged by size:
2441 1701 1639 1102 832 832 663 643
563 386 327 318 251 40 32 32
32 32 32 32 32 32 32 32
32 32 31 30 30 19 13 12
2 1
HincII GTy'rAC
Cuts at: 2632 3021 3608 3983 9299 2632
Size: 389 587 375 5316 5593
Fragments arranged by size:
5593 5316 587 389 375
HindIII A'AGCT_T
Cuts at: 1945 1945
Size: 12260
HpaI GTT'AAC
Cuts at: 3608 3608
Size: 12260
KasI G'GCGC_C
Cuts at: 3087 9690 9938 3087
Size: 6603 248 5409
Fragments arranged by size:
6603 5409 248
KpnI G_GTAC'C
Cuts at: 4510 4741 9280 4510
Size: 231 4539 7490
Fragments arranged by size:
7490 4539 231
MfeI C'AATT_G
Cuts at: 5590 5590
Size: 12260
MluI A'CGCG_T
Cuts at: 11693 11693
Size: 12260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 487 1049 1148 1286 4540 4723 4813 5304 8119
Size: 562 99 138 3254 183 90 491 2815
Cuts at: 8119 8437 8855 10931 11115 487
Size: 318 418 2076 184 1632
Fragments arranged by size:
3254 2815 2076 1632 562 491 418 318
184 183 138 99 90
MscI TGG'CCA
Cuts at: 634 1843 4093 4231 5813 6032 6133 6265 7273
Size: 1209 2250 138 1582 219 101 132 1008
Cuts at: 7273 10021 634
Size: 2748 2873
Fragments arranged by size:
2873 2748 2250 1582 1209 1008 219 138
132 101
MslI CAynn'nnrTG
Cuts at: 1112 1226 2068 2719 3673 5764 7894 8821 10249
Size: 114 842 651 954 2091 2130 927 1428
Cuts at: 10249 10376 1112
Size: 127 2996
Fragments arranged by size:
2996 2130 2091 1428 954 927 842 651
127 114
MspA1I CmG'CkG
Cuts at: 567 850 1065 1294 1810 2612 2727 2856 3450
Size: 283 215 229 516 802 115 129 594
Cuts at: 3450 3583 4021 5253 5469 6017 6489 6848 7651
Size: 133 438 1232 216 548 472 359 803
Cuts at: 7651 7875 9371 9685 10045 11058 11303 567
Size: 224 1496 314 360 1013 245 1524
Fragments arranged by size:
1524 1496 1232 1013 803 802 594 548
516 472 438 360 359 314 283 245
229 224 216 215 133 129 115
NaeI GCC'GGC
Cuts at: 3619 6424 9148 10441 3619
Size: 2805 2724 1293 5438
Fragments arranged by size:
5438 2805 2724 1293
NarI GG'CG_CC
Cuts at: 3088 9691 9939 3088
Size: 6603 248 5409
Fragments arranged by size:
6603 5409 248
NcoI C'CATG_G
Cuts at: 629 1328 1367 3467 5072 5808 5993 8816 629
Size: 699 39 2100 1605 736 185 2823 4073
Fragments arranged by size:
4073 2823 2100 1605 736 699 185 39
NgoMIV G'CCGG_C
Cuts at: 3617 6422 9146 10439 3617
Size: 2805 2724 1293 5438
Fragments arranged by size:
5438 2805 2724 1293
NruI TCG'CGA
Cuts at: 2441 2441
Size: 12260
NspI r_CATG'y
Cuts at: 2605 4165 4199 5539 5649 7953 9313 9813 10720
Size: 1560 34 1340 110 2304 1360 500 907
Cuts at: 10720 2605
Size: 4145
Fragments arranged by size:
4145 2304 1560 1360 1340 907 500 110
34
PasI CC'CwG_GG
Cuts at: 3093 3787 4686 4956 8251 8416 8697 9051 3093
Size: 694 899 270 3295 165 281 354 6302
Fragments arranged by size:
6302 3295 899 694 354 281 270 165
PciI A'CATG_T
Cuts at: 4161 10716 4161
Size: 6555 5705
PflMI CCAn_nnn'nTGG
Cuts at: 1414 5408 7236 8251 9001 1414
Size: 3994 1828 1015 750 4673
Fragments arranged by size:
4673 3994 1828 1015 750
PfoI T'CCnGG_A
Cuts at: 1892 4026 4476 5330 5582 6521 8285 1892
Size: 2134 450 854 252 939 1764 5867
Fragments arranged by size:
5867 2134 1764 939 854 450 252
PmeI GTTT'AAAC
Cuts at: 9259 9259
Size: 12260
PmlI CAC'GTG
Cuts at: 3771 3771
Size: 12260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6607 6639 11399 11431 6607
Size: 32 4760 32 7436
Fragments arranged by size:
7436 4760 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 3124 4690 7580 7702 9119 2041
Size: 1083 1566 2890 122 1417 5182
Fragments arranged by size:
5182 2890 1566 1417 1083 122
PshAI GACnn'nnGTC
Cuts at: 2867 5326 6017 6745 2867
Size: 2459 691 728 8382
Fragments arranged by size:
8382 2459 728 691
PspOMI G'GGCC_C
Cuts at: 294 584 1480 1759 3096 3208 3969 4845 4959
Size: 290 896 279 1337 112 761 876 114
Cuts at: 4959 5300 7984 8308 8990 294
Size: 341 2684 324 682 3564
Fragments arranged by size:
3564 2684 1337 896 876 761 682 341
324 290 279 114 112
PspXI vC'TCGA_Gb
Cuts at: 3647 3647
Size: 12260
PstI C_TGCA'G
Cuts at: 1806 2228 2617 3035 3266 6853 8436 9307 9992
Size: 422 389 418 231 3587 1583 871 685
Cuts at: 9992 1806
Size: 4074
Fragments arranged by size:
4074 3587 1583 871 685 422 418 389
231
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12260
PvuII CAG'CTG
Cuts at: 850 1065 1294 1810 2727 3583 5469 6489 7651
Size: 215 229 516 917 856 1886 1020 1162
Cuts at: 7651 9685 10045 850
Size: 2034 360 3065
Fragments arranged by size:
3065 2034 1886 1162 1020 917 856 516
360 229 215
RsrII CG'GwC_CG
Cuts at: 10455 10455
Size: 12260
SacI G_AGCT'C
Cuts at: 3134 3571 5606 6560 7526 9274 3134
Size: 437 2035 954 966 1748 6120
Fragments arranged by size:
6120 2035 1748 966 954 437
SalI G'TCGA_C
Cuts at: 9297 9297
Size: 12260
SanDI GG'GwC_CC
Cuts at: 2041 4690 9119 2041
Size: 2649 4429 5182
Fragments arranged by size:
5182 4429 2649
SapI GCTCTTCn'nnn_
Cuts at: 304 828 846 1031 1145 1490 1709 6566 7583
Size: 524 18 185 114 345 219 4857 1017
Cuts at: 7583 10283 10493 304
Size: 2700 210 2071
Fragments arranged by size:
4857 2700 2071 1017 524 345 219 210
185 114 18
SbfI CC_TGCA'GG
Cuts at: 9307 9307
Size: 12260
ScaI AGT'ACT
Cuts at: 766 1696 11777 766
Size: 930 10081 1249
Fragments arranged by size:
10081 1249 930
SexAI A'CCwGG_T
Cuts at: 4125 4125
Size: 12260
SfcI C'TryA_G
Cuts at: 33 1286 1802 2224 2613 3031 3262 3851 6849
Size: 1253 516 422 389 418 231 589 2998
Cuts at: 6849 8432 9034 9303 9988 10981 11172 33
Size: 1583 602 269 685 993 191 1121
Fragments arranged by size:
2998 1583 1253 1121 993 685 602 589
516 422 418 389 269 231 191
SfoI GGC'GCC
Cuts at: 3089 9692 9940 3089
Size: 6603 248 5409
Fragments arranged by size:
6603 5409 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12260
SgrAI Cr'CCGG_yG
Cuts at: 6701 6701
Size: 12260
SmaI CCC'GGG
Cuts at: 5614 6040 6454 6772 7928 9282 5614
Size: 426 414 318 1156 1354 8592
Fragments arranged by size:
8592 1354 1156 426 414 318
SmlI C'TyrA_G
Cuts at: 194 700 1349 2158 3647 3688 4932 5667 5728
Size: 506 649 809 1489 41 1244 735 61
Cuts at: 5728 6782 7203 8662 9415 10330 10822 11084 194
Size: 1054 421 1459 753 915 492 262 1370
Fragments arranged by size:
1489 1459 1370 1244 1054 915 809 753
735 649 506 492 421 262 61 41
SpeI A'CTAG_T
Cuts at: 67 7915 67
Size: 7848 4412
SphI G_CATG'C
Cuts at: 4199 5539 9313 9813 4199
Size: 1340 3774 500 6646
Fragments arranged by size:
6646 3774 1340 500
SrfI GCCC'GGGC
Cuts at: 6772 6772
Size: 12260
StuI AGG'CCT
Cuts at: 2239 2541 3681 5863 6529 6891 7209 2239
Size: 302 1140 2182 666 362 318 7290
Fragments arranged by size:
7290 2182 1140 666 362 318 302
StyI C'CwwG_G
Cuts at: 629 635 1216 1328 1367 1826 3467 4040 4979
Size: 6 581 112 39 459 1641 573 939
Cuts at: 4979 5072 5096 5554 5808 5993 6344 6611 6886
Size: 93 24 458 254 185 351 267 275
Cuts at: 6886 7196 7394 8703 8816 9168 9394 9748 629
Size: 310 198 1309 113 352 226 354 3141
Fragments arranged by size:
3141 1641 1309 939 581 573 459 458
354 352 351 310 275 267 254 226
198 185 113 112 93 39 24 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3681 4644 5561 5668 5776 6352 6712 7398 8492
Size: 963 917 107 108 576 360 686 1094
Cuts at: 8492 8586 9843 9976 10435 10644 3681
Size: 94 1257 133 459 209 5297
Fragments arranged by size:
5297 1257 1094 963 917 686 576 459
360 209 133 108 107 94
TatI w'GTAC_w
Cuts at: 764 1694 5439 11775 764
Size: 930 3745 6336 1249
Fragments arranged by size:
6336 3745 1249 930
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 442 474 501 533 3760 3792 3888 3917 3920
Size: 32 27 32 3227 32 96 29 3
Cuts at: 3920 3949 3998 4030 5913 5945 7053 7085 11777
Size: 29 49 32 1883 32 1108 32 4692
Cuts at: 11777 11809 442
Size: 32 893
Fragments arranged by size:
4692 3227 1883 1108 893 96 49 32
32 32 32 32 32 32 29 29
27 3
Tth111I GACn'n_nGTC
Cuts at: 4710 4758 4800 8245 10057 4710
Size: 48 42 3445 1812 6913
Fragments arranged by size:
6913 3445 1812 48 42
XbaI T'CTAG_A
Cuts at: 9291 9291
Size: 12260
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 246 3148 3633 3669 6519 7392 7743 9755 246
Size: 2902 485 36 2850 873 351 2012 2751
Fragments arranged by size:
2902 2850 2751 2012 873 485 351 36
XhoI C'TCGA_G
Cuts at: 1349 3647 8662 1349
Size: 2298 5015 4947
Fragments arranged by size:
5015 4947 2298
XmaI C'CCGG_G
Cuts at: 5612 6038 6452 6770 7926 9280 5612
Size: 426 414 318 1156 1354 8592
Fragments arranged by size:
8592 1354 1156 426 414 318
XmnI GAAnn'nnTTC
Cuts at: 621 621
Size: 12260
ZraI GAC'GTC
Cuts at: 9133 9133
Size: 12260
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BfrBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI
NdeI NheI NotI NsiI PacI PsiI PsrI SacII
SfiI SnaBI SspI SwaI