Restriction map for
pF1KA0308
(Circular) (Six-base) MAPSORT of: pF1KA0308.seq Check: 2574 from: 1 to: 9149
pF1KA0308 9149 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 1358 6187 1358
Size: 4829 4320
Acc65I G'GTAC_C
Cuts at: 2433 6165 2433
Size: 3732 5417
AclI AA'CG_TT
Cuts at: 251 8989 251
Size: 8738 411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 643 1291 1874 2150 3647 3950 4280 5452
Size: 346 648 583 276 1497 303 330 1172
Cuts at: 5452 6974 7406 8153 8421 297
Size: 1522 432 747 268 1025
Fragments arranged by size:
1522 1497 1172 1025 747 648 583 432
346 330 303 276 268
AflII C'TTAA_G
Cuts at: 585 4990 585
Size: 4405 4744
AflIII A'CryG_T
Cuts at: 1264 2797 5130 6501 7605 8582 1264
Size: 1533 2333 1371 1104 977 1831
Fragments arranged by size:
2333 1831 1533 1371 1104 977
AgeI A'CCGG_T
Cuts at: 7537 7537
Size: 9149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4559 5531 4559
Size: 972 8177
AlwNI CAG_nnn'CTG
Cuts at: 2179 2605 3862 4180 5389 5392 5505 6454 8021
Size: 426 1257 318 1209 3 113 949 1567
Cuts at: 8021 2179
Size: 3307
Fragments arranged by size:
3307 1567 1257 1209 949 426 318 113
3
ApaLI G'TGCA_C
Cuts at: 7919 7919
Size: 9149
ApoI r'AATT_y
Cuts at: 226 619 928 1567 2849 3052 4889 5220 6153
Size: 393 309 639 1282 203 1837 331 933
Cuts at: 6153 8502 8758 226
Size: 2349 256 617
Fragments arranged by size:
2349 1837 1282 933 639 617 393 331
309 256 203
AseI AT'TA_AT
Cuts at: 20 4922 20
Size: 4902 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9149
AvaI C'yCGr_G
Cuts at: 1223 2269 5001 6169 1223
Size: 1046 2732 1168 4203
Fragments arranged by size:
4203 2732 1168 1046
AvrII C'CTAG_G
Cuts at: 3544 3544
Size: 9149
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 821 854 821
Size: 33 9116
BamHI G'GATC_C
Cuts at: 3171 6174 3171
Size: 3003 6146
BanI G'GyrC_C
Cuts at: 526 720 2433 2877 4411 4699 6165 6579 6827
Size: 194 1713 444 1534 288 1466 414 248
Cuts at: 6827 6862 526
Size: 35 2813
Fragments arranged by size:
2813 1713 1534 1466 444 414 288 248
194 35
BanII G_rGCy'C
Cuts at: 869 2052 4391 6163 7193 869
Size: 1183 2339 1772 1030 2825
Fragments arranged by size:
2825 2339 1772 1183 1030
BbeI G_GCGC'C
Cuts at: 6583 6831 6583
Size: 248 8901
BbsI GAAGACnn'nnnn_
Cuts at: 556 3139 3614 3635 4577 5064 5678 6017 6146
Size: 2583 475 21 942 487 614 339 129
Cuts at: 6146 556
Size: 3559
Fragments arranged by size:
3559 2583 942 614 487 475 339 129
21
BbvCI CC'TCA_GC
Cuts at: 908 908
Size: 9149
BciVI GTATCCnnnnn_n'
Cuts at: 7047 7808 9149 7047
Size: 761 1341 7047
Fragments arranged by size:
7047 1341 761
BclI T'GATC_A
Cuts at: 4244 4434 6669 4244
Size: 190 2235 6724
Fragments arranged by size:
6724 2235 190
BglII A'GATC_T
Cuts at: 1509 2718 6664 8701 1509
Size: 1209 3946 2037 1957
Fragments arranged by size:
3946 2037 1957 1209
BlpI GC'TnA_GC
Cuts at: 6261 6261
Size: 9149
Bme1580I G_kGCm'C
Cuts at: 6774 6867 7923 6774
Size: 93 1056 8000
Fragments arranged by size:
8000 1056 93
BmgBI CAC'GTC
Cuts at: 1639 1639
Size: 9149
BmrI ACTGGGnnnn_n'
Cuts at: 225 1429 4385 5907 6361 6442 6777 8921 225
Size: 1204 2956 1522 454 81 335 2144 453
Fragments arranged by size:
2956 2144 1522 1204 454 453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7516 7516
Size: 9149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 3737 4385 5141 5573 5864 1111
Size: 2626 648 756 432 291 4396
Fragments arranged by size:
4396 2626 756 648 432 291
Bpu10I CC'TnA_GC
Cuts at: 868 908 868
Size: 40 9109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4868 6325 7204 7696 7994 4868
Size: 1457 879 492 298 6023
Fragments arranged by size:
6023 1457 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1887 3713 4627 8853 1887
Size: 1826 914 4226 2183
Fragments arranged by size:
4226 2183 1826 914
BsaAI yAC'GTr
Cuts at: 236 6502 236
Size: 6266 2883
BsaBI GATnn'nnATC
Cuts at: 6 965 1289 1586 3834 6663 8700 6
Size: 959 324 297 2248 2829 2037 455
Fragments arranged by size:
2829 2248 2037 959 455 324 297
BsaHI Gr'CG_yC
Cuts at: 2808 6580 6828 7530 9026 2808
Size: 3772 248 702 1496 2931
Fragments arranged by size:
3772 2931 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1682 1889 6338 6859 7537 7811 7958 8369 1682
Size: 207 4449 521 678 274 147 411 2462
Fragments arranged by size:
4449 2462 678 521 411 274 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1884 1914 2708 2738 3126 3156 3533 3563 3604
Size: 30 794 30 388 30 377 30 41
Cuts at: 3604 3634 5243 5273 1884
Size: 30 1609 30 5760
Fragments arranged by size:
5760 1609 794 388 377 41 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 356 925 2096 3518 3592 3625 3993 3996 3999
Size: 569 1171 1422 74 33 368 3 3
Cuts at: 3999 4002 4824 5570 356
Size: 3 822 746 3935
Fragments arranged by size:
3935 1422 1171 822 746 569 368 74
33 3 3 3
BseYI C'CCAG_C
Cuts at: 2553 2670 3795 5518 6574 7332 7909 8405 2553
Size: 117 1125 1723 1056 758 577 496 3297
Fragments arranged by size:
3297 1723 1125 1056 758 577 496 117
BsiEI CG_ry'CG
Cuts at: 104 6691 6737 7945 104
Size: 6587 46 1208 1308
Fragments arranged by size:
6587 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 869 1055 2052 4126 4581 5000 6163 6941 7131
Size: 186 997 2074 455 419 1163 778 190
Cuts at: 7131 7923 869
Size: 792 2095
Fragments arranged by size:
2095 2074 1163 997 792 778 455 419
190 186
BsiWI C'GTAC_G
Cuts at: 232 232
Size: 9149
BsmI GAATG_Cn'
Cuts at: 1936 2351 3115 5045 5537 1936
Size: 415 764 1930 492 5548
Fragments arranged by size:
5548 1930 764 492 415
BsmBI CGTCTCn'nnnn_
Cuts at: 681 2815 5340 681
Size: 2134 2525 4490
Fragments arranged by size:
4490 2525 2134
Bsp1286I G_dGCh'C
Cuts at: 869 1055 2052 4126 4391 4581 5000 6163 6774
Size: 186 997 2074 265 190 419 1163 611
Cuts at: 6774 6867 6941 7131 7193 7923 869
Size: 93 74 190 62 730 2095
Fragments arranged by size:
2095 2074 1163 997 730 611 419 265
190 190 186 93 74 62
BspEI T'CCGG_A
Cuts at: 1889 8369 1889
Size: 6480 2669
BspHI T'CATG_A
Cuts at: 494 3345 5049 8325 494
Size: 2851 1704 3276 1318
Fragments arranged by size:
3276 2851 1704 1318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1961 2673 6199 6413 6715 7096 1961
Size: 712 3526 214 302 381 4014
Fragments arranged by size:
4014 3526 712 381 302 214
BsrBI CCG'CTC
Cuts at: 50 7441 7495 8979 9144 50
Size: 7391 54 1484 165 55
Fragments arranged by size:
7391 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 983 7061 983
Size: 6078 3071
BsrFI r'CCGG_y
Cuts at: 4414 5539 7147 7328 7537 4414
Size: 1125 1608 181 209 6026
Fragments arranged by size:
6026 1608 1125 209 181
BsrGI T'GTAC_A
Cuts at: 5931 5931
Size: 9149
BssSI C'ACGA_G
Cuts at: 2144 7420 7778 2144
Size: 5276 358 3515
Fragments arranged by size:
5276 3515 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2605 3520 5389 5392 5505 6526 2605
Size: 915 1869 3 113 1021 5228
Fragments arranged by size:
5228 1869 1021 915 113 3
BstBI TT'CG_AA
Cuts at: 1518 2256 7510 1518
Size: 738 5254 3157
Fragments arranged by size:
5254 3157 738
BstXI CCAn_nnnn'nTGG
Cuts at: 991 1333 3329 3730 991
Size: 342 1996 401 6410
Fragments arranged by size:
6410 1996 401 342
BstYI r'GATC_y
Cuts at: 1 627 1509 2112 2649 2718 3171 5454 6091
Size: 626 882 603 537 69 453 2283 637
Cuts at: 6091 6174 6641 6664 6999 7245 8257 8343 8355
Size: 83 467 23 335 246 1012 86 12
Cuts at: 8355 8701 1
Size: 346 449
Fragments arranged by size:
2283 1012 882 637 626 603 537 467
453 449 346 335 246 86 83 69
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 9149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 438 7099 7280 7443 438
Size: 6661 181 163 2144
Fragments arranged by size:
6661 2144 181 163
BtsI GCAGTG_nn'
Cuts at: 2816 5503 6392 2816
Size: 2687 889 5573
Fragments arranged by size:
5573 2687 889
ClaI AT'CG_AT
Cuts at: 1586 1586
Size: 9149
DraI TTT'AAA
Cuts at: 2514 2682 6148 2514
Size: 168 3466 5515
Fragments arranged by size:
5515 3466 168
DraIII CAC_nnn'GTG
Cuts at: 513 3384 513
Size: 2871 6278
DrdI GACnn_nn'nnGTC
Cuts at: 4176 6855 7713 4176
Size: 2679 858 5612
Fragments arranged by size:
5612 2679 858
EaeI y'GGCC_r
Cuts at: 1998 4225 6734 6908 7299 7326 8671 1998
Size: 2227 2509 174 391 27 1345 2476
Fragments arranged by size:
2509 2476 2227 1345 391 174 27
EagI C'GGCC_G
Cuts at: 6734 6734
Size: 9149
EarI CTCTTCn'nnn_
Cuts at: 1452 1515 1571 2964 3915 3990 4002 4020 5471
Size: 63 56 1393 951 75 12 18 1451
Cuts at: 5471 7172 7382 1452
Size: 1701 210 3219
Fragments arranged by size:
3219 1701 1451 1393 951 210 75 63
56 18 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7357 7667 7813 8959 7357
Size: 310 146 1146 7547
Fragments arranged by size:
7547 1146 310 146
EcoICRI GAG'CTC
Cuts at: 867 2050 6161 867
Size: 1183 4111 3855
Fragments arranged by size:
4111 3855 1183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 643 1111 1291 1874 2150 3647 3737 3950
Size: 346 468 180 583 276 1497 90 213
Cuts at: 3950 4280 4385 5141 5452 5573 5864 6974 7406
Size: 330 105 756 311 121 291 1110 432
Cuts at: 7406 8153 8421 297
Size: 747 268 1025
Fragments arranged by size:
1497 1110 1025 756 747 583 468 432
346 330 311 291 276 268 213 180
121 105 90
EcoNI CCTnn'n_nnAGG
Cuts at: 3029 3607 5552 3029
Size: 578 1945 6626
Fragments arranged by size:
6626 1945 578
EcoO109I rG'GnC_Cy
Cuts at: 175 483 3261 6288 175
Size: 308 2778 3027 3036
Fragments arranged by size:
3036 3027 2778 308
EcoRI G'AATT_C
Cuts at: 4889 6153 4889
Size: 1264 7885
EcoRV GAT'ATC
Cuts at: 1584 1584
Size: 9149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 194 611 769 1531 1544 2935 3532 5789 7168
Size: 417 158 762 13 1391 597 2257 1379
Cuts at: 7168 194
Size: 2175
Fragments arranged by size:
2257 2175 1391 1379 762 597 417 158
13
FspI TGC'GCA
Cuts at: 6930 6930
Size: 9149
HaeII r_GCGC'y
Cuts at: 6583 6831 7853 8832 6583
Size: 248 1022 979 6900
Fragments arranged by size:
6900 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 177 209 1546 1578 1975 1977 2007 2009 2692
Size: 32 1337 32 397 2 30 2 683
Cuts at: 2692 2724 3126 3158 3210 3242 3604 3636 3712
Size: 32 402 32 52 32 362 32 76
Cuts at: 3712 3744 3838 3856 3870 3888 5941 5973 6070
Size: 32 94 18 14 18 2053 32 97
Cuts at: 6070 6088 6102 6120 6991 7021 7023 7053 177
Size: 18 14 18 871 30 2 30 2273
Fragments arranged by size:
2273 2053 1337 871 683 402 397 362
97 94 76 52 32 32 32 32
32 32 32 32 30 30 30 18
18 18 18 14 14 2 2 2
HincII GTy'rAC
Cuts at: 1646 2746 4442 4523 4776 6188 1646
Size: 1100 1696 81 253 1412 4607
Fragments arranged by size:
4607 1696 1412 1100 253 81
HindIII A'AGCT_T
Cuts at: 3295 3295
Size: 9149
KasI G'GCGC_C
Cuts at: 6579 6827 6579
Size: 248 8901
KpnI G_GTAC'C
Cuts at: 2437 6169 2437
Size: 3732 5417
MfeI C'AATT_G
Cuts at: 413 1622 413
Size: 1209 7940
MluI A'CGCG_T
Cuts at: 8582 8582
Size: 9149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2149 3112 4235 5148 5926 7820 8004 2149
Size: 963 1123 913 778 1894 184 3294
Fragments arranged by size:
3294 1894 1123 963 913 778 184
MscI TGG'CCA
Cuts at: 4227 6910 4227
Size: 2683 6466
MslI CAynn'nnrTG
Cuts at: 2522 3124 7138 7265 2522
Size: 602 4014 127 4406
Fragments arranged by size:
4406 4014 602 127
MspA1I CmG'CkG
Cuts at: 952 1258 5389 5806 5812 6260 6574 6934 7947
Size: 306 4131 417 6 448 314 360 1013
Cuts at: 7947 8192 952
Size: 245 1909
Fragments arranged by size:
4131 1909 1013 448 417 360 314 306
245 6
NaeI GCC'GGC
Cuts at: 7330 7330
Size: 9149
NarI GG'CG_CC
Cuts at: 6580 6828 6580
Size: 248 8901
NcoI C'CATG_G
Cuts at: 107 107
Size: 9149
NdeI CA'TA_TG
Cuts at: 1383 2107 1383
Size: 724 8425
NgoMIV G'CCGG_C
Cuts at: 7328 7328
Size: 9149
NspI r_CATG'y
Cuts at: 1268 4471 5292 6202 6702 7609 1268
Size: 3203 821 910 500 907 2808
Fragments arranged by size:
3203 2808 910 907 821 500
PasI CC'CwG_GG
Cuts at: 2186 2186
Size: 9149
PciI A'CATG_T
Cuts at: 1264 7605 1264
Size: 6341 2808
PflMI CCAn_nnn'nTGG
Cuts at: 1715 3694 4564 4696 1715
Size: 1979 870 132 6168
Fragments arranged by size:
6168 1979 870 132
PmeI GTTT'AAAC
Cuts at: 6148 6148
Size: 9149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5288 5320 5718 5750 8288 8320 5288
Size: 32 398 32 2538 32 6117
Fragments arranged by size:
6117 2538 398 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 175 175
Size: 9149
PshAI GACnn'nnGTC
Cuts at: 1641 1641
Size: 9149
PsiI TTA'TAA
Cuts at: 898 898
Size: 9149
PstI C_TGCA'G
Cuts at: 1257 5811 6196 6881 1257
Size: 4554 385 685 3525
Fragments arranged by size:
4554 3525 685 385
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9149
PvuII CAG'CTG
Cuts at: 952 1258 5389 5806 5812 6574 6934 952
Size: 306 4131 417 6 762 360 3167
Fragments arranged by size:
4131 3167 762 417 360 306 6
RsrII CG'GwC_CG
Cuts at: 7344 7344
Size: 9149
SacI G_AGCT'C
Cuts at: 869 2052 6163 869
Size: 1183 4111 3855
Fragments arranged by size:
4111 3855 1183
SalI G'TCGA_C
Cuts at: 6186 6186
Size: 9149
SapI GCTCTTCn'nnn_
Cuts at: 7172 7382 7172
Size: 210 8939
SbfI CC_TGCA'GG
Cuts at: 6196 6196
Size: 9149
ScaI AGT'ACT
Cuts at: 4077 8666 4077
Size: 4589 4560
SfcI C'TryA_G
Cuts at: 33 1169 1253 1359 2152 3450 3496 4373 4730
Size: 1136 84 106 793 1298 46 877 357
Cuts at: 4730 5807 5969 6192 6877 7870 8061 33
Size: 1077 162 223 685 993 191 1121
Fragments arranged by size:
1298 1136 1121 1077 993 877 793 685
357 223 191 162 106 84 46
SfoI GGC'GCC
Cuts at: 6581 6829 6581
Size: 248 8901
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9149
SmaI CCC'GGG
Cuts at: 5003 6171 5003
Size: 1168 7981
SmlI C'TyrA_G
Cuts at: 585 4883 4990 6304 7219 7711 7973 585
Size: 4298 107 1314 915 492 262 1761
Fragments arranged by size:
4298 1761 1314 915 492 262 107
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9149
SphI G_CATG'C
Cuts at: 6202 6702 6202
Size: 500 8649
SspI AAT'ATT
Cuts at: 762 3106 3180 762
Size: 2344 74 6731
Fragments arranged by size:
6731 2344 74
StuI AGG'CCT
Cuts at: 2168 5473 5885 2168
Size: 3305 412 5432
Fragments arranged by size:
5432 3305 412
StyI C'CwwG_G
Cuts at: 107 1655 3544 4695 6283 6637 107
Size: 1548 1889 1151 1588 354 2619
Fragments arranged by size:
2619 1889 1588 1548 1151 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1418 1955 6732 6865 7324 7533 1418
Size: 537 4777 133 459 209 3034
Fragments arranged by size:
4777 3034 537 459 209 133
TatI w'GTAC_w
Cuts at: 308 4075 5931 6100 8664 308
Size: 3767 1856 169 2564 793
Fragments arranged by size:
3767 2564 1856 793 169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1884 1916 2907 2939 8666 8698 1884
Size: 32 991 32 5727 32 2335
Fragments arranged by size:
5727 2335 991 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6946 6946
Size: 9149
XbaI T'CTAG_A
Cuts at: 3573 6180 3573
Size: 2607 6542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1416 3162 3730 4350 6644 1416
Size: 1746 568 620 2294 3921
Fragments arranged by size:
3921 2294 1746 620 568
XmaI C'CCGG_G
Cuts at: 5001 6169 5001
Size: 1168 7981
XmnI GAAnn'nnTTC
Cuts at: 155 383 2252 5132 5300 155
Size: 228 1869 2880 168 4004
Fragments arranged by size:
4004 2880 1869 228 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
BcgI BfrBI BglI BmtI BsgI BssHII BstEII BstZ17I
Bsu36I CspCI FseI FspAI HpaI NheI NotI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI