Restriction map for
pF1KA0305
(Circular) (Six-base) MAPSORT of: pF1KA0305.seq Check: 9800 from: 1 to: 7730
pF1KA0305 7730 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2634 3817 3990 4235 4768 2634
Size: 1183 173 245 533 5596
Fragments arranged by size:
5596 1183 533 245 173
Acc65I G'GTAC_C
Cuts at: 4746 4746
Size: 7730
AclI AA'CG_TT
Cuts at: 7570 7570
Size: 7730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 422 593 1175 1423 1717 2264 2816 3053
Size: 198 171 582 248 294 547 552 237
Cuts at: 3053 3435 4082 4295 4610 5555 5987 6340 7002
Size: 382 647 213 315 945 432 353 662
Cuts at: 7002 224
Size: 952
Fragments arranged by size:
952 945 662 647 582 552 547 432
382 353 315 294 248 237 213 198
171
AflII C'TTAA_G
Cuts at: 1159 2569 1159
Size: 1410 6320
AflIII A'CryG_T
Cuts at: 836 1200 4213 5082 6882 7163 836
Size: 364 3013 869 1800 281 1403
Fragments arranged by size:
3013 1800 1403 869 364 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2611 2643 2611
Size: 32 7698
AlwNI CAG_nnn'CTG
Cuts at: 2017 3031 3214 4588 5035 6473 2017
Size: 1014 183 1374 447 1438 3274
Fragments arranged by size:
3274 1438 1374 1014 447 183
ApaLI G'TGCA_C
Cuts at: 6568 6568
Size: 7730
ApoI r'AATT_y
Cuts at: 783 1059 1074 2383 3322 3632 3918 4004 4573
Size: 276 15 1309 939 310 286 86 569
Cuts at: 4573 4734 7083 7339 783
Size: 161 2349 256 1174
Fragments arranged by size:
2349 1309 1174 939 569 310 286 276
256 161 86 15
AseI AT'TA_AT
Cuts at: 20 1270 1447 1607 1883 20
Size: 1250 177 160 276 5867
Fragments arranged by size:
5867 1250 276 177 160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7730
AvaI C'yCGr_G
Cuts at: 4750 4750
Size: 7730
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2144 2177 2144
Size: 33 7697
BamHI G'GATC_C
Cuts at: 4755 4755
Size: 7730
BanI G'GyrC_C
Cuts at: 4746 5160 5408 5443 4746
Size: 414 248 35 7033
Fragments arranged by size:
7033 414 248 35
BanII G_rGCy'C
Cuts at: 4086 4744 5774 4086
Size: 658 1030 6042
Fragments arranged by size:
6042 1030 658
BbeI G_GCGC'C
Cuts at: 5164 5412 5164
Size: 248 7482
BbsI GAAGACnn'nnnn_
Cuts at: 1145 1231 1469 1574 2804 3139 1145
Size: 86 238 105 1230 335 5736
Fragments arranged by size:
5736 1230 335 238 105 86
BbvCI CC'TCA_GC
Cuts at: 1304 4607 1304
Size: 3303 4427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2393 2427 2393
Size: 34 7696
BciVI GTATCCnnnnn_n'
Cuts at: 5628 6684 7730 5628
Size: 1056 1046 5628
Fragments arranged by size:
5628 1056 1046
BclI T'GATC_A
Cuts at: 5250 5250
Size: 7730
BfrBI ATG'CAT
Cuts at: 211 211
Size: 7730
BglII A'GATC_T
Cuts at: 800 5245 7282 800
Size: 4445 2037 1248
Fragments arranged by size:
4445 2037 1248
BlpI GC'TnA_GC
Cuts at: 4842 4842
Size: 7730
Bme1580I G_kGCm'C
Cuts at: 4498 5355 5448 6572 4498
Size: 857 93 1124 5656
Fragments arranged by size:
5656 1124 857 93
BmrI ACTGGGnnnn_n'
Cuts at: 4942 5023 5358 7502 4942
Size: 81 335 2144 5170
Fragments arranged by size:
5170 2144 335 81
BmtI G_CTAG'C
Cuts at: 4183 4183
Size: 7730
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6097 6097
Size: 7730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3215 4139 3215
Size: 924 6806
Bpu10I CC'TnA_GC
Cuts at: 1304 2054 2528 4607 1304
Size: 750 474 2079 4427
Fragments arranged by size:
4427 2079 750 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1532 3670 4906 5785 6499 6797 346
Size: 1186 2138 1236 879 714 298 1279
Fragments arranged by size:
2138 1279 1236 1186 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4153 7434 4153
Size: 3281 4449
BsaAI yAC'GTr
Cuts at: 4251 5083 4251
Size: 832 6898
BsaBI GATnn'nnATC
Cuts at: 6 3977 5244 7281 6
Size: 3971 1267 2037 455
Fragments arranged by size:
3971 2037 1267 455
BsaHI Gr'CG_yC
Cuts at: 5161 5409 6111 7607 5161
Size: 248 702 1496 5284
Fragments arranged by size:
5284 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4919 5440 6118 6529 6676 6950 4919
Size: 521 678 411 147 274 5699
Fragments arranged by size:
5699 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2600 3716 2600
Size: 1116 6614
BseYI C'CCAG_C
Cuts at: 2140 4262 5155 5913 6578 6986 2140
Size: 2122 893 758 665 408 2884
Fragments arranged by size:
2884 2122 893 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1115 3722 1115
Size: 2607 5123
BsiEI CG_ry'CG
Cuts at: 104 5272 5318 6548 104
Size: 5168 46 1230 1286
Fragments arranged by size:
5168 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 4086 4638 4744 5522 5712 6572 4086
Size: 552 106 778 190 860 5244
Fragments arranged by size:
5244 860 778 552 190 106
Bsp1286I G_dGCh'C
Cuts at: 4086 4498 4638 4744 5355 5448 5522 5712 5774
Size: 412 140 106 611 93 74 190 62
Cuts at: 5774 6572 4086
Size: 798 5244
Fragments arranged by size:
5244 798 611 412 190 140 106 93
74 62
BspHI T'CATG_A
Cuts at: 3237 6162 3237
Size: 2925 4805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1024 4780 4994 5296 5677 1024
Size: 3756 214 302 381 3077
Fragments arranged by size:
3756 3077 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6022 6076 7560 7725 50
Size: 5972 54 1484 165 55
Fragments arranged by size:
5972 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 268 1629 2409 5642 268
Size: 1361 780 3233 2356
Fragments arranged by size:
3233 2356 1361 780
BsrFI r'CCGG_y
Cuts at: 5728 5909 5728
Size: 181 7549
BsrGI T'GTAC_A
Cuts at: 201 201
Size: 7730
BssSI C'ACGA_G
Cuts at: 3310 6001 6709 3310
Size: 2691 708 4331
Fragments arranged by size:
4331 2691 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3721 5107 3721
Size: 1386 6344
BstBI TT'CG_AA
Cuts at: 3804 6091 3804
Size: 2287 5443
BstEII G'GTnAC_C
Cuts at: 1973 2981 1973
Size: 1008 6722
BstXI CCAn_nnnn'nTGG
Cuts at: 913 913
Size: 7730
BstYI r'GATC_y
Cuts at: 1 800 1476 2251 4585 4755 5222 5245 5580
Size: 799 676 775 2334 170 467 23 335
Cuts at: 5580 5826 6132 6144 6230 7282 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2334 1052 799 775 676 467 449 335
306 246 170 86 23 12
BstZ17I GTA'TAC
Cuts at: 2635 3818 3991 2635
Size: 1183 173 6374
Fragments arranged by size:
6374 1183 173
Bsu36I CC'TnA_GG
Cuts at: 2612 3440 2612
Size: 828 6902
BtgI C'CryG_G
Cuts at: 107 3883 4472 4653 107
Size: 3776 589 181 3184
Fragments arranged by size:
3776 3184 589 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5680 5861 6024 5680
Size: 181 163 7386
Fragments arranged by size:
7386 181 163
BtsI GCAGTG_nn'
Cuts at: 225 1343 4973 225
Size: 1118 3630 2982
Fragments arranged by size:
3630 2982 1118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4049 4084 4049
Size: 35 7695
DraI TTT'AAA
Cuts at: 123 788 986 4206 4729 123
Size: 665 198 3220 523 3124
Fragments arranged by size:
3220 3124 665 523 198
DraIII CAC_nnn'GTG
Cuts at: 1291 1291
Size: 7730
DrdI GACnn_nn'nnGTC
Cuts at: 5436 6780 5436
Size: 1344 6386
EaeI y'GGCC_r
Cuts at: 4035 5315 5489 5880 5907 7252 4035
Size: 1280 174 391 27 1345 4513
Fragments arranged by size:
4513 1345 1280 391 174 27
EagI C'GGCC_G
Cuts at: 5315 5315
Size: 7730
EarI CTCTTCn'nnn_
Cuts at: 986 1194 2704 5753 5963 986
Size: 208 1510 3049 210 2753
Fragments arranged by size:
3049 2753 1510 210 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5938 6680 6826 7540 5938
Size: 742 146 714 6128
Fragments arranged by size:
6128 742 714 146
EcoICRI GAG'CTC
Cuts at: 4084 4742 4084
Size: 658 7072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 422 593 1175 1423 1717 2264 2816 3053
Size: 198 171 582 248 294 547 552 237
Cuts at: 3053 3215 3435 4082 4139 4295 4610 5555 5987
Size: 162 220 647 57 156 315 945 432
Cuts at: 5987 6340 7002 224
Size: 353 662 952
Fragments arranged by size:
952 945 662 647 582 552 547 432
353 315 294 248 237 220 198 171
162 156 57
EcoO109I rG'GnC_Cy
Cuts at: 2097 4661 4869 2097
Size: 2564 208 4958
Fragments arranged by size:
4958 2564 208
EcoRI G'AATT_C
Cuts at: 3632 4734 3632
Size: 1102 6628
EcoRV GAT'ATC
Cuts at: 4257 4257
Size: 7730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1573 3465 3635 4615 5749 1573
Size: 1892 170 980 1134 3554
Fragments arranged by size:
3554 1892 1134 980 170
FspI TGC'GCA
Cuts at: 5511 5511
Size: 7730
HaeII r_GCGC'y
Cuts at: 5164 5412 6642 7413 5164
Size: 248 1230 771 5481
Fragments arranged by size:
5481 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 792 824 1207 1239 1765 1797 1986
Size: 32 204 32 383 32 526 32 189
Cuts at: 1986 2018 2535 2567 3446 3478 3586 3618 4303
Size: 32 517 32 879 32 108 32 685
Cuts at: 4303 4335 5572 5602 5604 5634 556
Size: 32 1237 30 2 30 2652
Fragments arranged by size:
2652 1237 879 685 526 517 383 204
189 108 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3066 3798 4769 3066
Size: 732 971 6027
Fragments arranged by size:
6027 971 732
HindIII A'AGCT_T
Cuts at: 3646 3646
Size: 7730
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 7730
KasI G'GCGC_C
Cuts at: 5160 5408 5160
Size: 248 7482
KpnI G_GTAC'C
Cuts at: 4750 4750
Size: 7730
MfeI C'AATT_G
Cuts at: 821 2059 821
Size: 1238 6492
MluI A'CGCG_T
Cuts at: 7163 7163
Size: 7730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2019 2578 4691 6489 6673 2019
Size: 559 2113 1798 184 3076
Fragments arranged by size:
3076 2113 1798 559 184
MscI TGG'CCA
Cuts at: 4037 5491 4037
Size: 1454 6276
MslI CAynn'nnrTG
Cuts at: 650 2585 3781 4564 5719 5846 650
Size: 1935 1196 783 1155 127 2534
Fragments arranged by size:
2534 1935 1196 1155 783 127
MspA1I CmG'CkG
Cuts at: 130 2191 4841 5155 5515 6299 6544 130
Size: 2061 2650 314 360 784 245 1316
Fragments arranged by size:
2650 2061 1316 784 360 314 245
NaeI GCC'GGC
Cuts at: 5911 5911
Size: 7730
NarI GG'CG_CC
Cuts at: 5161 5409 5161
Size: 248 7482
NcoI C'CATG_G
Cuts at: 107 4472 4653 107
Size: 4365 181 3184
Fragments arranged by size:
4365 3184 181
NdeI CA'TA_TG
Cuts at: 213 3851 213
Size: 3638 4092
NgoMIV G'CCGG_C
Cuts at: 5909 5909
Size: 7730
NheI G'CTAG_C
Cuts at: 4179 4179
Size: 7730
NsiI A_TGCA'T
Cuts at: 213 213
Size: 7730
NspI r_CATG'y
Cuts at: 840 1204 2423 3937 4217 4783 5283 6886 840
Size: 364 1219 1514 280 566 500 1603 1684
Fragments arranged by size:
1684 1603 1514 1219 566 500 364 280
PasI CC'CwG_GG
Cuts at: 3659 3659
Size: 7730
PciI A'CATG_T
Cuts at: 836 1200 4213 6882 836
Size: 364 3013 2669 1684
Fragments arranged by size:
3013 2669 1684 364
PmeI GTTT'AAAC
Cuts at: 4729 4729
Size: 7730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6176 6208 6176
Size: 32 7698
PpuMI rG'GwC_Cy
Cuts at: 2097 4661 2097
Size: 2564 5166
PshAI GACnn'nnGTC
Cuts at: 2804 2804
Size: 7730
PsiI TTA'TAA
Cuts at: 1120 1120
Size: 7730
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2775 2807 2775
Size: 32 7698
PstI C_TGCA'G
Cuts at: 1021 4777 5462 1021
Size: 3756 685 3289
Fragments arranged by size:
3756 3289 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7730
PvuII CAG'CTG
Cuts at: 130 2191 5155 5515 130
Size: 2061 2964 360 2345
Fragments arranged by size:
2964 2345 2061 360
RsrII CG'GwC_CG
Cuts at: 5925 5925
Size: 7730
SacI G_AGCT'C
Cuts at: 4086 4744 4086
Size: 658 7072
SalI G'TCGA_C
Cuts at: 4767 4767
Size: 7730
SapI GCTCTTCn'nnn_
Cuts at: 5753 5963 5753
Size: 210 7520
SbfI CC_TGCA'GG
Cuts at: 4777 4777
Size: 7730
ScaI AGT'ACT
Cuts at: 3914 7247 3914
Size: 3333 4397
SexAI A'CCwGG_T
Cuts at: 2676 2676
Size: 7730
SfcI C'TryA_G
Cuts at: 33 1017 1532 2177 2186 2864 3215 4773 5458
Size: 984 515 645 9 678 351 1558 685
Cuts at: 5458 6426 6617 33
Size: 968 191 1146
Fragments arranged by size:
1558 1146 984 968 685 678 645 515
351 191 9
SfoI GGC'GCC
Cuts at: 5162 5410 5162
Size: 248 7482
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7730
SmaI CCC'GGG
Cuts at: 4752 4752
Size: 7730
SmlI C'TyrA_G
Cuts at: 361 1159 1547 2569 3649 4885 5800 6514 6776
Size: 798 388 1022 1080 1236 915 714 262
Cuts at: 6776 361
Size: 1315
Fragments arranged by size:
1315 1236 1080 1022 915 798 714 388
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7730
SphI G_CATG'C
Cuts at: 4783 5283 4783
Size: 500 7230
SspI AAT'ATT
Cuts at: 1659 3349 3516 1659
Size: 1690 167 5873
Fragments arranged by size:
5873 1690 167
StyI C'CwwG_G
Cuts at: 107 4472 4653 4864 5218 107
Size: 4365 181 211 354 2619
Fragments arranged by size:
4365 2619 354 211 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3688 5313 5446 5905 6114 3688
Size: 1625 133 459 209 5304
Fragments arranged by size:
5304 1625 459 209 133
TatI w'GTAC_w
Cuts at: 201 2593 3893 3912 3961 7245 201
Size: 2392 1300 19 49 3284 686
Fragments arranged by size:
3284 2392 1300 686 49 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4023 4055 7247 7279 4023
Size: 32 3192 32 4474
Fragments arranged by size:
4474 3192 32 32
Tth111I GACn'n_nGTC
Cuts at: 5527 5527
Size: 7730
XbaI T'CTAG_A
Cuts at: 2475 4761 2475
Size: 2286 5444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5225 5225
Size: 7730
XmaI C'CCGG_G
Cuts at: 4750 4750
Size: 7730
XmnI GAAnn'nnTTC
Cuts at: 3272 3272
Size: 7730
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
AscI AvrII BglI BmgBI BsaXI BsiWI BsmI BsmBI
BspEI BssHII ClaI EcoNI FseI FspAI NotI NruI
PacI PflMI PfoI PmlI PspOMI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI