Restriction map for
pF1KA0296
(Circular) (Six-base) MAPSORT of: pF1KA0296.seq Check: 0 from: 1 to: 8600
pF1KA0296 8600 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3085 4105 4262 4483 4925 5401 3085
Size: 1020 157 221 442 476 6284
Fragments arranged by size:
6284 1020 476 442 221 157
AatII G_ACGT'C
Cuts at: 5107 5107
Size: 8600
AccI GT'mk_AC
Cuts at: 1307 5638 1307
Size: 4331 4269
Acc65I G'GTAC_C
Cuts at: 5616 5616
Size: 8600
AclI AA'CG_TT
Cuts at: 8440 8440
Size: 8600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 2022 2225 2285 2330 3699 3713 4150 6425
Size: 1052 203 60 45 1369 14 437 2275
Cuts at: 6425 6857 7210 7872 970
Size: 432 353 662 1698
Fragments arranged by size:
2275 1698 1369 1052 662 437 432 353
203 60 45 14
AfeI AGC'GCT
Cuts at: 2957 2957
Size: 8600
AflII C'TTAA_G
Cuts at: 5263 5263
Size: 8600
AflIII A'CryG_T
Cuts at: 5952 7752 8033 5952
Size: 1800 281 6519
Fragments arranged by size:
6519 1800 281
AleI CACnn'nnGTG
Cuts at: 2476 2984 4195 4607 2476
Size: 508 1211 412 6469
Fragments arranged by size:
6469 1211 508 412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1654 3490 4213 4943 1654
Size: 1836 723 730 5311
Fragments arranged by size:
5311 1836 730 723
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4526 4558 4526
Size: 32 8568
AlwNI CAG_nnn'CTG
Cuts at: 646 747 1158 2503 2630 2887 3215 4146 4905
Size: 101 411 1345 127 257 328 931 759
Cuts at: 4905 5315 5905 7343 646
Size: 410 590 1438 1903
Fragments arranged by size:
1903 1438 1345 931 759 590 411 410
328 257 127 101
ApaI G_GGCC'C
Cuts at: 3446 3488 3446
Size: 42 8558
ApaLI G'TGCA_C
Cuts at: 1148 7438 1148
Size: 6290 2310
ApoI r'AATT_y
Cuts at: 3144 5604 7953 8209 3144
Size: 2460 2349 256 3535
Fragments arranged by size:
3535 2460 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8600
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8600
AvaI C'yCGr_G
Cuts at: 286 1025 2195 3986 4931 5375 5585 5620 286
Size: 739 1170 1791 945 444 210 35 3266
Fragments arranged by size:
3266 1791 1170 945 739 444 210 35
AvrII C'CTAG_G
Cuts at: 1948 1948
Size: 8600
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 813 846 813
Size: 33 8567
BamHI G'GATC_C
Cuts at: 5625 5625
Size: 8600
BanI G'GyrC_C
Cuts at: 182 1690 2146 2807 3193 3970 4250 4947 5616
Size: 1508 456 661 386 777 280 697 669
Cuts at: 5616 6030 6278 6313 182
Size: 414 248 35 2469
Fragments arranged by size:
2469 1508 777 697 669 661 456 414
386 280 248 35
BanII G_rGCy'C
Cuts at: 176 696 788 1178 1374 2845 3434 3446 3488
Size: 520 92 390 196 1471 589 12 42
Cuts at: 3488 3587 3659 4435 5585 5614 6644 176
Size: 99 72 776 1150 29 1030 2132
Fragments arranged by size:
2132 1471 1150 1030 776 589 520 390
196 99 92 72 42 29 12
BbeI G_GCGC'C
Cuts at: 2811 6034 6282 2811
Size: 3223 248 5129
Fragments arranged by size:
5129 3223 248
BbsI GAAGACnn'nnnn_
Cuts at: 1005 1868 2213 2492 1005
Size: 863 345 279 7113
Fragments arranged by size:
7113 863 345 279
BbvCI CC'TCA_GC
Cuts at: 806 1685 2736 5117 806
Size: 879 1051 2381 4289
Fragments arranged by size:
4289 2381 1051 879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4763 4797 4763
Size: 34 8566
BciVI GTATCCnnnnn_n'
Cuts at: 6498 7554 8600 6498
Size: 1056 1046 6498
Fragments arranged by size:
6498 1056 1046
BclI T'GATC_A
Cuts at: 6120 6120
Size: 8600
BglI GCCn_nnn'nGGC
Cuts at: 1994 2765 3299 3797 4040 4301 4310 4327 4715
Size: 771 534 498 243 261 9 17 388
Cuts at: 4715 4736 5035 5174 1994
Size: 21 299 139 5420
Fragments arranged by size:
5420 771 534 498 388 299 261 243
139 21 17 9
BglII A'GATC_T
Cuts at: 600 6115 8152 600
Size: 5515 2037 1048
Fragments arranged by size:
5515 2037 1048
BlpI GC'TnA_GC
Cuts at: 1177 2617 3248 4376 5712 1177
Size: 1440 631 1128 1336 4065
Fragments arranged by size:
4065 1440 1336 1128 631
Bme1580I G_kGCm'C
Cuts at: 1115 1152 1695 2981 3446 3488 3973 5521 6225
Size: 37 543 1286 465 42 485 1548 704
Cuts at: 6225 6318 7442 1115
Size: 93 1124 2273
Fragments arranged by size:
2273 1548 1286 1124 704 543 485 465
93 42 37
BmrI ACTGGGnnnn_n'
Cuts at: 786 969 1139 1723 2387 2483 4528 5000 5142
Size: 183 170 584 664 96 2045 472 142
Cuts at: 5142 5812 5893 6228 8372 786
Size: 670 81 335 2144 1014
Fragments arranged by size:
2144 2045 1014 670 664 584 472 335
183 170 142 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 2697 2751 3229 3361 6967 211
Size: 2486 54 478 132 3606 1844
Fragments arranged by size:
3606 2486 1844 478 132 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1216 1231 1595 2242 3046 3176 3356 3547
Size: 774 15 364 647 804 130 180 191
Cuts at: 3547 3610 3685 5368 442
Size: 63 75 1683 3674
Fragments arranged by size:
3674 1683 804 774 647 364 191 180
130 75 63 15
Bpu10I CC'TnA_GC
Cuts at: 806 1685 2736 5117 806
Size: 879 1051 2381 4289
Fragments arranged by size:
4289 2381 1051 879
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1690 2350 3164 3826 5776 6655 7369 7667
Size: 1329 660 814 662 1950 879 714 298
Cuts at: 7667 361
Size: 1294
Fragments arranged by size:
1950 1329 1294 879 814 714 662 660
298
BsaI GGTCTCn'nnnn_
Cuts at: 1441 1924 2335 3622 4351 8304 1441
Size: 483 411 1287 729 3953 1737
Fragments arranged by size:
3953 1737 1287 729 483 411
BsaAI yAC'GTr
Cuts at: 593 1638 5953 593
Size: 1045 4315 3240
Fragments arranged by size:
4315 3240 1045
BsaBI GATnn'nnATC
Cuts at: 6 6114 8151 6
Size: 6108 2037 455
Fragments arranged by size:
6108 2037 455
BsaHI Gr'CG_yC
Cuts at: 2808 4850 5104 6031 6279 6981 8477 2808
Size: 2042 254 927 248 702 1496 2931
Fragments arranged by size:
2931 2042 1496 927 702 254 248
BsaWI w'CCGG_w
Cuts at: 5482 5789 6310 6988 7399 7546 7820 5482
Size: 307 521 678 411 147 274 6262
Fragments arranged by size:
6262 678 521 411 307 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 384 414 805 835 4526 4556 5057 5087 5209
Size: 30 391 30 3691 30 501 30 122
Cuts at: 5209 5239 384
Size: 30 3745
Fragments arranged by size:
3745 3691 501 391 122 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 689 823 826 1099 2508 2814 2863 2866
Size: 451 134 3 273 1409 306 49 3
Cuts at: 2866 3544 3640 3661 4282 4582 4689 4978 5476
Size: 678 96 21 621 300 107 289 498
Cuts at: 5476 5524 5589 238
Size: 48 65 3249
Fragments arranged by size:
3249 1409 678 621 498 451 306 300
289 273 134 107 96 65 49 48
21 3 3
BseYI C'CCAG_C
Cuts at: 160 515 665 711 894 1079 1264 1696 1806
Size: 355 150 46 183 185 185 432 110
Cuts at: 1806 2557 3076 3515 4538 4652 6025 6783 7448
Size: 751 519 439 1023 114 1373 758 665
Cuts at: 7448 7856 160
Size: 408 904
Fragments arranged by size:
1373 1023 904 758 751 665 519 439
432 408 355 185 185 183 150 114
110 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 1527 1691 2873 2987 4132 4502 5127 5458
Size: 850 164 1182 114 1145 370 625 331
Cuts at: 5458 677
Size: 3819
Fragments arranged by size:
3819 1182 1145 850 625 370 331 164
114
BsiEI CG_ry'CG
Cuts at: 104 6142 6188 7418 104
Size: 6038 46 1230 1286
Fragments arranged by size:
6038 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 176 1152 1178 1591 2659 5227 5614 6392 6582
Size: 976 26 413 1068 2568 387 778 190
Cuts at: 6582 7442 176
Size: 860 1334
Fragments arranged by size:
2568 1334 1068 976 860 778 413 387
190 26
BsmI GAATG_Cn'
Cuts at: 2589 2589
Size: 8600
BsmBI CGTCTCn'nnnn_
Cuts at: 460 4066 460
Size: 3606 4994
Bsp1286I G_dGCh'C
Cuts at: 176 696 788 1115 1152 1178 1374 1591 1695
Size: 520 92 327 37 26 196 217 104
Cuts at: 1695 2659 2845 2981 3434 3446 3488 3587 3659
Size: 964 186 136 453 12 42 99 72
Cuts at: 3659 3973 4435 5227 5521 5585 5614 6225 6318
Size: 314 462 792 294 64 29 611 93
Cuts at: 6318 6392 6582 6644 7442 176
Size: 74 190 62 798 1334
Fragments arranged by size:
1334 964 798 792 611 520 462 453
327 314 294 217 196 190 186 136
104 99 93 92 74 72 64 62
42 37 29 26 12
BspHI T'CATG_A
Cuts at: 7032 7032
Size: 8600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3085 3451 3468 4105 4262 4483 4925 5261 5401
Size: 366 17 637 157 221 442 336 140
Cuts at: 5401 5650 5864 6166 6547 3085
Size: 249 214 302 381 5138
Fragments arranged by size:
5138 637 442 381 366 336 302 249
221 214 157 140 17
BsrBI CCG'CTC
Cuts at: 50 816 2369 2566 3708 4248 5130 6892 6946
Size: 766 1553 197 1142 540 882 1762 54
Cuts at: 6946 8430 8595 50
Size: 1484 165 55
Fragments arranged by size:
1762 1553 1484 1142 882 766 540 197
165 55 54
BsrDI GCAATG_nn'
Cuts at: 1647 6512 1647
Size: 4865 3735
BsrFI r'CCGG_y
Cuts at: 1220 2099 2804 3751 3770 3787 3851 4039 4058
Size: 879 705 947 19 17 64 188 19
Cuts at: 4058 4503 5476 6598 6779 1220
Size: 445 973 1122 181 3041
Fragments arranged by size:
3041 1122 973 947 879 705 445 188
181 64 19 19 17
BssSI C'ACGA_G
Cuts at: 316 4069 6871 7579 316
Size: 3753 2802 708 1337
Fragments arranged by size:
3753 2802 1337 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2887 3100 5150 5977 2887
Size: 213 2050 827 5510
Fragments arranged by size:
5510 2050 827 213
BstBI TT'CG_AA
Cuts at: 6961 6961
Size: 8600
BstXI CCAn_nnnn'nTGG
Cuts at: 322 369 499 1485 1573 1620 2175 2608 2722
Size: 47 130 986 88 47 555 433 114
Cuts at: 2722 3084 3111 3334 4122 4369 4475 4624 322
Size: 362 27 223 788 247 106 149 4298
Fragments arranged by size:
4298 986 788 555 433 362 247 223
149 130 114 106 88 47 47 27
BstYI r'GATC_y
Cuts at: 1 600 3323 3867 4237 4974 5625 6092 6115
Size: 599 2723 544 370 737 651 467 23
Cuts at: 6115 6450 6696 7002 7014 7100 8152 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2723 1052 737 651 599 544 467 449
370 335 306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 404 647 404
Size: 243 8357
BtgI C'CryG_G
Cuts at: 107 368 1793 2072 3104 3294 5108 5355 107
Size: 261 1425 279 1032 190 1814 247 3352
Fragments arranged by size:
3352 1814 1425 1032 279 261 247 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1540 6550 6731 6894 1540
Size: 5010 181 163 3246
Fragments arranged by size:
5010 3246 181 163
BtsI GCAGTG_nn'
Cuts at: 268 999 1515 2902 3474 4021 5301 5843 268
Size: 731 516 1387 572 547 1280 542 3025
Fragments arranged by size:
3025 1387 1280 731 572 547 542 516
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3258 3293 4684 4719 5535 5570 3258
Size: 35 1391 35 816 35 6288
Fragments arranged by size:
6288 1391 816 35 35 35
DraI TTT'AAA
Cuts at: 5599 5599
Size: 8600
DraIII CAC_nnn'GTG
Cuts at: 5150 5525 5150
Size: 375 8225
DrdI GACnn_nn'nnGTC
Cuts at: 1938 6306 7650 1938
Size: 4368 1344 2888
Fragments arranged by size:
4368 2888 1344
EaeI y'GGCC_r
Cuts at: 1245 2505 3599 3732 3789 3926 4215 4230 4293
Size: 1260 1094 133 57 137 289 15 63
Cuts at: 4293 4319 4501 4817 4907 5474 6185 6359 6750
Size: 26 182 316 90 567 711 174 391
Cuts at: 6750 6777 8122 1245
Size: 27 1345 1723
Fragments arranged by size:
1723 1345 1260 1094 711 567 391 316
289 182 174 137 133 90 63 57
27 26 15
EagI C'GGCC_G
Cuts at: 6185 6185
Size: 8600
EarI CTCTTCn'nnn_
Cuts at: 536 1460 1781 2526 2855 3608 6623 6833 536
Size: 924 321 745 329 753 3015 210 2303
Fragments arranged by size:
3015 2303 924 753 745 329 321 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2130 3898 4038 5407 6808 7550 7696 8410 2130
Size: 1768 140 1369 1401 742 146 714 2320
Fragments arranged by size:
2320 1768 1401 1369 742 714 146 140
EcoICRI GAG'CTC
Cuts at: 174 1176 5612 174
Size: 1002 4436 3162
Fragments arranged by size:
4436 3162 1002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 970 1216 1231 1595 2022 2225 2242 2285
Size: 528 246 15 364 427 203 17 43
Cuts at: 2285 2330 3046 3176 3356 3547 3610 3685 3699
Size: 45 716 130 180 191 63 75 14
Cuts at: 3699 3713 4150 5368 6425 6857 7210 7872 442
Size: 14 437 1218 1057 432 353 662 1170
Fragments arranged by size:
1218 1170 1057 716 662 528 437 432
427 364 353 246 203 191 180 130
75 63 45 43 17 15 14 14
EcoNI CCTnn'n_nnAGG
Cuts at: 5018 5018
Size: 8600
EcoO109I rG'GnC_Cy
Cuts at: 425 643 670 1470 1952 2048 2627 5037 5279
Size: 218 27 800 482 96 579 2410 242
Cuts at: 5279 5383 5501 5739 425
Size: 104 118 238 3286
Fragments arranged by size:
3286 2410 800 579 482 242 238 218
118 104 96 27
EcoRI G'AATT_C
Cuts at: 3144 5604 3144
Size: 2460 6140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2109 4821 6619 2109
Size: 2712 1798 4090
Fragments arranged by size:
4090 2712 1798
FspI TGC'GCA
Cuts at: 1630 6381 1630
Size: 4751 3849
HaeII r_GCGC'y
Cuts at: 153 2811 2959 5160 5301 6034 6282 7512 8283
Size: 2658 148 2201 141 733 248 1230 771
Cuts at: 8283 153
Size: 470
Fragments arranged by size:
2658 2201 1230 771 733 470 248 148
141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 457 489 544 576 3160 3192 4456
Size: 32 41 32 55 32 2584 32 1264
Cuts at: 4456 4488 4657 4689 5026 5058 6442 6472 6474
Size: 32 169 32 337 32 1384 30 2
Cuts at: 6474 6504 384
Size: 30 2480
Fragments arranged by size:
2584 2480 1384 1264 337 169 55 41
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 300 5639 300
Size: 5339 3261
HpaI GTT'AAC
Cuts at: 300 300
Size: 8600
KasI G'GCGC_C
Cuts at: 2807 6030 6278 2807
Size: 3223 248 5129
Fragments arranged by size:
5129 3223 248
KpnI G_GTAC'C
Cuts at: 5620 5620
Size: 8600
MfeI C'AATT_G
Cuts at: 4363 4363
Size: 8600
MluI A'CGCG_T
Cuts at: 8033 8033
Size: 8600
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 164 289 3221 4139 7359 7543 164
Size: 125 2932 918 3220 184 1221
Fragments arranged by size:
3220 2932 1221 918 184 125
MscI TGG'CCA
Cuts at: 2507 3601 3928 4295 4819 4909 6361 2507
Size: 1094 327 367 524 90 1452 4746
Fragments arranged by size:
4746 1452 1094 524 367 327 90
MslI CAynn'nnrTG
Cuts at: 1058 1118 2476 2984 3109 4195 4607 6589 6716
Size: 60 1358 508 125 1086 412 1982 127
Cuts at: 6716 1058
Size: 2942
Fragments arranged by size:
2942 1982 1358 1086 508 412 127 125
60
MspA1I CmG'CkG
Cuts at: 134 1232 1335 2153 2182 2937 2943 3266 3365
Size: 1098 103 818 29 755 6 323 99
Cuts at: 3365 3719 3884 3924 3939 4007 4088 4195 4318
Size: 354 165 40 15 68 81 107 123
Cuts at: 4318 4680 5141 5338 5711 6025 6385 7169 7414
Size: 362 461 197 373 314 360 784 245
Cuts at: 7414 134
Size: 1320
Fragments arranged by size:
1320 1098 818 784 755 461 373 362
360 354 323 314 245 197 165 123
107 103 99 81 68 40 29 15
6
NaeI GCC'GGC
Cuts at: 2101 3753 4041 5478 6781 2101
Size: 1652 288 1437 1303 3920
Fragments arranged by size:
3920 1652 1437 1303 288
NarI GG'CG_CC
Cuts at: 2808 6031 6279 2808
Size: 3223 248 5129
Fragments arranged by size:
5129 3223 248
NcoI C'CATG_G
Cuts at: 107 368 2072 3104 3294 5108 107
Size: 261 1704 1032 190 1814 3599
Fragments arranged by size:
3599 1814 1704 1032 261 190
NdeI CA'TA_TG
Cuts at: 386 386
Size: 8600
NgoMIV G'CCGG_C
Cuts at: 2099 3751 4039 5476 6779 2099
Size: 1652 288 1437 1303 3920
Fragments arranged by size:
3920 1652 1437 1303 288
NspI r_CATG'y
Cuts at: 400 973 2576 5653 6153 7756 400
Size: 573 1603 3077 500 1603 1244
Fragments arranged by size:
3077 1603 1603 1244 573 500
PasI CC'CwG_GG
Cuts at: 575 2779 575
Size: 2204 6396
PciI A'CATG_T
Cuts at: 7752 7752
Size: 8600
PflMI CCAn_nnn'nTGG
Cuts at: 894 2073 2503 3522 4291 4905 894
Size: 1179 430 1019 769 614 4589
Fragments arranged by size:
4589 1179 1019 769 614 430
PfoI T'CCnGG_A
Cuts at: 3563 3563
Size: 8600
PmeI GTTT'AAAC
Cuts at: 5599 5599
Size: 8600
PmlI CAC'GTG
Cuts at: 1638 1638
Size: 8600
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 7046 7078 4526
Size: 32 2488 32 6048
Fragments arranged by size:
6048 2488 32 32
PpuMI rG'GwC_Cy
Cuts at: 643 670 1470 5279 5501 643
Size: 27 800 3809 222 3742
Fragments arranged by size:
3809 3742 800 222 27
PshAI GACnn'nnGTC
Cuts at: 1303 1303
Size: 8600
PspOMI G'GGCC_C
Cuts at: 3442 3484 3442
Size: 42 8558
PstI C_TGCA'G
Cuts at: 2909 2942 3066 3479 3889 4685 4922 5311 5647
Size: 33 124 413 410 796 237 389 336
Cuts at: 5647 6332 2909
Size: 685 5177
Fragments arranged by size:
5177 796 685 413 410 389 336 237
124 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8600
PvuII CAG'CTG
Cuts at: 134 1335 2937 3719 3924 4088 4195 4680 5338
Size: 1201 1602 782 205 164 107 485 658
Cuts at: 5338 6025 6385 134
Size: 687 360 2349
Fragments arranged by size:
2349 1602 1201 782 687 658 485 360
205 164 107
RsrII CG'GwC_CG
Cuts at: 6795 6795
Size: 8600
SacI G_AGCT'C
Cuts at: 176 1178 5614 176
Size: 1002 4436 3162
Fragments arranged by size:
4436 3162 1002
SalI G'TCGA_C
Cuts at: 5637 5637
Size: 8600
SanDI GG'GwC_CC
Cuts at: 670 5501 670
Size: 4831 3769
SapI GCTCTTCn'nnn_
Cuts at: 6623 6833 6623
Size: 210 8390
SbfI CC_TGCA'GG
Cuts at: 4922 5647 4922
Size: 725 7875
ScaI AGT'ACT
Cuts at: 8117 8117
Size: 8600
SexAI A'CCwGG_T
Cuts at: 5000 5000
Size: 8600
SfcI C'TryA_G
Cuts at: 33 1836 2793 2905 2938 3062 3475 3885 4681
Size: 1803 957 112 33 124 413 410 796
Cuts at: 4681 4918 5307 5643 6328 7296 7487 33
Size: 237 389 336 685 968 191 1146
Fragments arranged by size:
1803 1146 968 957 796 685 413 410
389 336 237 191 124 112 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 4301 4301
Size: 8600
SfoI GGC'GCC
Cuts at: 2809 6032 6280 2809
Size: 3223 248 5129
Fragments arranged by size:
5129 3223 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8600
SgrAI Cr'CCGG_yG
Cuts at: 2804 3851 4058 2804
Size: 1047 207 7346
Fragments arranged by size:
7346 1047 207
SmaI CCC'GGG
Cuts at: 288 1027 2197 4933 5377 5587 5622 288
Size: 739 1170 2736 444 210 35 3266
Fragments arranged by size:
3266 2736 1170 739 444 210 35
SmlI C'TyrA_G
Cuts at: 376 1705 2329 3179 3841 5263 5755 6670 7384
Size: 1329 624 850 662 1422 492 915 714
Cuts at: 7384 7646 376
Size: 262 1330
Fragments arranged by size:
1422 1330 1329 915 850 714 662 624
492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8600
SphI G_CATG'C
Cuts at: 973 5653 6153 973
Size: 4680 500 3420
Fragments arranged by size:
4680 3420 500
StuI AGG'CCT
Cuts at: 2219 2721 3808 3904 4304 5326 2219
Size: 502 1087 96 400 1022 5493
Fragments arranged by size:
5493 1087 1022 502 400 96
StyI C'CwwG_G
Cuts at: 107 368 437 1948 2072 3083 3104 3200 3294
Size: 261 69 1511 124 1011 21 96 94
Cuts at: 3294 3815 4408 4796 5108 5734 6088 107
Size: 521 593 388 312 626 354 2619
Fragments arranged by size:
2619 1511 1011 626 593 521 388 354
312 261 124 96 94 69 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 653 805 1468 2651 4357 4487 4787 5161 6183
Size: 152 663 1183 1706 130 300 374 1022
Cuts at: 6183 6316 6775 6984 653
Size: 133 459 209 2269
Fragments arranged by size:
2269 1706 1183 1022 663 459 374 300
209 152 133 130
TatI w'GTAC_w
Cuts at: 4082 8115 4082
Size: 4033 4567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1033 1065 1756 1788 2732 2764 4479 4511 4515
Size: 32 691 32 944 32 1715 32 4
Cuts at: 4515 4547 5057 5084 5089 5116 8117 8149 1033
Size: 32 510 27 5 27 3001 32 1484
Fragments arranged by size:
3001 1715 1484 944 691 510 32 32
32 32 32 32 27 27 5 4
Tth111I GACn'n_nGTC
Cuts at: 471 3124 4582 6397 471
Size: 2653 1458 1815 2674
Fragments arranged by size:
2674 2653 1815 1458
XbaI T'CTAG_A
Cuts at: 5631 5631
Size: 8600
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1482 2824 4396 6095 1482
Size: 1342 1572 1699 3987
Fragments arranged by size:
3987 1699 1572 1342
XmaI C'CCGG_G
Cuts at: 286 1025 2195 4931 5375 5585 5620 286
Size: 739 1170 2736 444 210 35 3266
Fragments arranged by size:
3266 2736 1170 739 444 210 35
XmnI GAAnn'nnTTC
Cuts at: 3904 3904
Size: 8600
ZraI GAC'GTC
Cuts at: 5105 5105
Size: 8600
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AhdI AscI BfrBI BmgBI BmtI BsiWI BspEI
BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI
HindIII NheI NotI NruI NsiI PacI PsiI PspXI
PsrI SacII SnaBI SrfI SspI SwaI XhoI