Restriction map for
pF1KA0266
(Circular) (Six-base) MAPSORT of: pF1KA0266.seq Check: 1805 from: 1 to: 5411
pF1KA0266 5411 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2449 2449
Size: 5411
Acc65I G'GTAC_C
Cuts at: 2427 2427
Size: 5411
AclI AA'CG_TT
Cuts at: 5251 5251
Size: 5411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 571 769 3236 3668 4415 4683 359
Size: 212 198 2467 432 747 268 1087
Fragments arranged by size:
2467 1087 747 432 268 212 198
AflII C'TTAA_G
Cuts at: 994 994
Size: 5411
AflIII A'CryG_T
Cuts at: 2380 2763 3867 4844 2380
Size: 383 1104 977 2947
Fragments arranged by size:
2947 1104 977 383
AgeI A'CCGG_T
Cuts at: 3799 3799
Size: 5411
AleI CACnn'nnGTG
Cuts at: 1156 1156
Size: 5411
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1157 1189 1157
Size: 32 5379
AlwNI CAG_nnn'CTG
Cuts at: 635 1238 2716 4283 635
Size: 603 1478 1567 1763
Fragments arranged by size:
1763 1567 1478 603
ApaLI G'TGCA_C
Cuts at: 4181 4181
Size: 5411
ApoI r'AATT_y
Cuts at: 665 1355 1369 2091 2415 4764 5020 665
Size: 690 14 722 324 2349 256 1056
Fragments arranged by size:
2349 1056 722 690 324 256 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 5411
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
AvaI C'yCGr_G
Cuts at: 794 2431 794
Size: 1637 3774
BamHI G'GATC_C
Cuts at: 2215 2436 2215
Size: 221 5190
BanI G'GyrC_C
Cuts at: 2427 2841 3089 3124 2427
Size: 414 248 35 4714
Fragments arranged by size:
4714 414 248 35
BanII G_rGCy'C
Cuts at: 383 795 801 1409 2425 3455 383
Size: 412 6 608 1016 1030 2339
Fragments arranged by size:
2339 1030 1016 608 412 6
BbeI G_GCGC'C
Cuts at: 2845 3093 2845
Size: 248 5163
BbsI GAAGACnn'nnnn_
Cuts at: 2318 2318
Size: 5411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1176 1210 1176
Size: 34 5377
BciVI GTATCCnnnnn_n'
Cuts at: 3309 4070 5411 3309
Size: 761 1341 3309
Fragments arranged by size:
3309 1341 761
BclI T'GATC_A
Cuts at: 1775 2931 1775
Size: 1156 4255
BglII A'GATC_T
Cuts at: 369 1399 2926 4963 369
Size: 1030 1527 2037 817
Fragments arranged by size:
2037 1527 1030 817
BlpI GC'TnA_GC
Cuts at: 1421 2523 1421
Size: 1102 4309
Bme1580I G_kGCm'C
Cuts at: 1488 3036 3129 4185 1488
Size: 1548 93 1056 2714
Fragments arranged by size:
2714 1548 1056 93
BmgBI CAC'GTC
Cuts at: 1974 2383 1974
Size: 409 5002
BmrI ACTGGGnnnn_n'
Cuts at: 2021 2623 2704 3039 5183 2021
Size: 602 81 335 2144 2249
Fragments arranged by size:
2249 2144 602 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3778 3778
Size: 5411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1075 1099 676
Size: 399 24 4988
Fragments arranged by size:
4988 399 24
Bpu10I CC'TnA_GC
Cuts at: 1280 1280
Size: 5411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 397 2316 2587 3466 3958 4256 207
Size: 190 1919 271 879 492 298 1362
Fragments arranged by size:
1919 1362 879 492 298 271 190
BsaI GGTCTCn'nnnn_
Cuts at: 1865 5115 1865
Size: 3250 2161
BsaAI yAC'GTr
Cuts at: 2764 2764
Size: 5411
BsaBI GATnn'nnATC
Cuts at: 6 2925 4962 6
Size: 2919 2037 455
Fragments arranged by size:
2919 2037 455
BsaHI Gr'CG_yC
Cuts at: 2043 2842 3090 3792 5288 2043
Size: 799 248 702 1496 2166
Fragments arranged by size:
2166 1496 799 702 248
BsaWI w'CCGG_w
Cuts at: 2600 3121 3799 4073 4220 4631 2600
Size: 521 678 274 147 411 3380
Fragments arranged by size:
3380 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1097 1127 1456 1486 1791 1821 1097
Size: 30 329 30 305 30 4687
Fragments arranged by size:
4687 329 305 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 1156 1861 1879 667
Size: 489 705 18 4199
Fragments arranged by size:
4199 705 489 18
BseYI C'CCAG_C
Cuts at: 357 1650 1903 1994 2836 3594 4171 4667 357
Size: 1293 253 91 842 758 577 496 1101
Fragments arranged by size:
1293 1101 842 758 577 496 253 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1224 1216
Size: 8 5403
BsiEI CG_ry'CG
Cuts at: 104 2953 2999 4207 104
Size: 2849 46 1208 1308
Fragments arranged by size:
2849 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1409 2425 3203 3393 4185 1409
Size: 1016 778 190 792 2635
Fragments arranged by size:
2635 1016 792 778 190
BsmI GAATG_Cn'
Cuts at: 502 502
Size: 5411
Bsp1286I G_dGCh'C
Cuts at: 383 795 801 1409 1488 2425 3036 3129 3203
Size: 412 6 608 79 937 611 93 74
Cuts at: 3203 3393 3455 4185 383
Size: 190 62 730 1609
Fragments arranged by size:
1609 937 730 611 608 412 190 93
79 74 62 6
BspEI T'CCGG_A
Cuts at: 4631 4631
Size: 5411
BspHI T'CATG_A
Cuts at: 1305 4587 1305
Size: 3282 2129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1584 2351 2461 2675 2977 3358 1584
Size: 767 110 214 302 381 3637
Fragments arranged by size:
3637 767 381 302 214 110
BsrBI CCG'CTC
Cuts at: 50 3703 3757 5241 5406 50
Size: 3653 54 1484 165 55
Fragments arranged by size:
3653 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 605 3323 605
Size: 2718 2693
BsrFI r'CCGG_y
Cuts at: 3409 3590 3799 3409
Size: 181 209 5021
Fragments arranged by size:
5021 209 181
BssSI C'ACGA_G
Cuts at: 3682 4040 3682
Size: 358 5053
BstAPI GCAn_nnn'nTGC
Cuts at: 2788 2788
Size: 5411
BstBI TT'CG_AA
Cuts at: 3772 3772
Size: 5411
BstXI CCAn_nnnn'nTGG
Cuts at: 753 978 1009 753
Size: 225 31 5155
Fragments arranged by size:
5155 225 31
BstYI r'GATC_y
Cuts at: 1 369 485 705 1399 2215 2436 2903 2926
Size: 368 116 220 694 816 221 467 23
Cuts at: 2926 3261 3507 4519 4605 4617 4963 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 816 694 467 449 368 346 335
246 221 220 116 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1748 2066 1748
Size: 318 5093
BtgI C'CryG_G
Cuts at: 722 722
Size: 5411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3361 3542 3705 3361
Size: 181 163 5067
Fragments arranged by size:
5067 181 163
BtsI GCAGTG_nn'
Cuts at: 2654 2654
Size: 5411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 428 463 1955 1990 428
Size: 35 1492 35 3849
Fragments arranged by size:
3849 1492 35 35
DraI TTT'AAA
Cuts at: 2410 2410
Size: 5411
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2347 3117 3975 1803
Size: 544 770 858 3239
Fragments arranged by size:
3239 858 770 544
EaeI y'GGCC_r
Cuts at: 404 1040 1085 2996 3170 3561 3588 4933 404
Size: 636 45 1911 174 391 27 1345 882
Fragments arranged by size:
1911 1345 882 636 391 174 45 27
EagI C'GGCC_G
Cuts at: 2996 2996
Size: 5411
EarI CTCTTCn'nnn_
Cuts at: 752 1133 1631 1640 1856 1933 3434 3644 752
Size: 381 498 9 216 77 1501 210 2519
Fragments arranged by size:
2519 1501 498 381 216 210 77 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 294 3619 3929 4075 5221 294
Size: 3325 310 146 1146 484
Fragments arranged by size:
3325 1146 484 310 146
EcoICRI GAG'CTC
Cuts at: 1407 2423 1407
Size: 1016 4395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 571 676 769 1075 1099 3236 3668 4415
Size: 212 105 93 306 24 2137 432 747
Cuts at: 4415 4683 359
Size: 268 1087
Fragments arranged by size:
2137 1087 747 432 306 268 212 105
93 24
EcoNI CCTnn'n_nnAGG
Cuts at: 589 658 757 589
Size: 69 99 5243
Fragments arranged by size:
5243 99 69
EcoO109I rG'GnC_Cy
Cuts at: 522 537 1275 1721 2071 2550 522
Size: 15 738 446 350 479 3383
Fragments arranged by size:
3383 738 479 446 350 15
EcoRI G'AATT_C
Cuts at: 2415 2415
Size: 5411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 912 1186 3430 912
Size: 274 2244 2893
Fragments arranged by size:
2893 2244 274
FspI TGC'GCA
Cuts at: 3192 3192
Size: 5411
HaeII r_GCGC'y
Cuts at: 2845 3093 4115 5094 2845
Size: 248 1022 979 3162
Fragments arranged by size:
3162 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 216 248 1391 1423 1456 1488 3253 3283 3285
Size: 32 1143 32 33 32 1765 30 2
Cuts at: 3285 3315 216
Size: 30 2312
Fragments arranged by size:
2312 1765 1143 33 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2450 2450
Size: 5411
HindIII A'AGCT_T
Cuts at: 238 1895 238
Size: 1657 3754
KasI G'GCGC_C
Cuts at: 2841 3089 2841
Size: 248 5163
KpnI G_GTAC'C
Cuts at: 2431 2431
Size: 5411
MluI A'CGCG_T
Cuts at: 4844 4844
Size: 5411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 955 4082 4266 955
Size: 3127 184 2100
Fragments arranged by size:
3127 2100 184
MscI TGG'CCA
Cuts at: 406 1042 1087 3172 406
Size: 636 45 2085 2645
Fragments arranged by size:
2645 2085 636 45
MslI CAynn'nnrTG
Cuts at: 112 1156 3400 3527 112
Size: 1044 2244 127 1996
Fragments arranged by size:
2244 1996 1044 127
MspA1I CmG'CkG
Cuts at: 573 2522 2836 3196 4209 4454 573
Size: 1949 314 360 1013 245 1530
Fragments arranged by size:
1949 1530 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3592 3592
Size: 5411
NarI GG'CG_CC
Cuts at: 2842 3090 2842
Size: 248 5163
NcoI C'CATG_G
Cuts at: 722 722
Size: 5411
NgoMIV G'CCGG_C
Cuts at: 3590 3590
Size: 5411
NspI r_CATG'y
Cuts at: 625 2464 2964 3871 625
Size: 1839 500 907 2165
Fragments arranged by size:
2165 1839 907 500
PasI CC'CwG_GG
Cuts at: 585 585
Size: 5411
PciI A'CATG_T
Cuts at: 3867 3867
Size: 5411
PfoI T'CCnGG_A
Cuts at: 1519 1519
Size: 5411
PmeI GTTT'AAAC
Cuts at: 2410 2410
Size: 5411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 611 643 4550 4582 611
Size: 32 3907 32 1440
Fragments arranged by size:
3907 1440 32 32
PpuMI rG'GwC_Cy
Cuts at: 522 537 1275 1721 2071 522
Size: 15 738 446 350 3862
Fragments arranged by size:
3862 738 446 350 15
PshAI GACnn'nnGTC
Cuts at: 1710 1710
Size: 5411
PstI C_TGCA'G
Cuts at: 806 2458 3143 806
Size: 1652 685 3074
Fragments arranged by size:
3074 1652 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5411
PvuII CAG'CTG
Cuts at: 573 2836 3196 573
Size: 2263 360 2788
Fragments arranged by size:
2788 2263 360
RsrII CG'GwC_CG
Cuts at: 1213 3606 1213
Size: 2393 3018
SacI G_AGCT'C
Cuts at: 1409 2425 1409
Size: 1016 4395
SalI G'TCGA_C
Cuts at: 2448 2448
Size: 5411
SanDI GG'GwC_CC
Cuts at: 537 537
Size: 5411
SapI GCTCTTCn'nnn_
Cuts at: 1631 1640 3434 3644 1631
Size: 9 1794 210 3398
Fragments arranged by size:
3398 1794 210 9
SbfI CC_TGCA'GG
Cuts at: 2458 2458
Size: 5411
ScaI AGT'ACT
Cuts at: 4928 4928
Size: 5411
SexAI A'CCwGG_T
Cuts at: 119 119
Size: 5411
SfcI C'TryA_G
Cuts at: 33 802 916 1606 1783 2210 2454 3139 4132
Size: 769 114 690 177 427 244 685 993
Cuts at: 4132 4323 33
Size: 191 1121
Fragments arranged by size:
1121 993 769 690 685 427 244 191
177 114
SfoI GGC'GCC
Cuts at: 2843 3091 2843
Size: 248 5163
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
SmaI CCC'GGG
Cuts at: 2433 2433
Size: 5411
SmlI C'TyrA_G
Cuts at: 186 376 994 2331 2566 3481 3973 4235 186
Size: 190 618 1337 235 915 492 262 1362
Fragments arranged by size:
1362 1337 915 618 492 262 235 190
SpeI A'CTAG_T
Cuts at: 67 159 67
Size: 92 5319
SphI G_CATG'C
Cuts at: 2464 2964 2464
Size: 500 4911
StuI AGG'CCT
Cuts at: 733 733
Size: 5411
StyI C'CwwG_G
Cuts at: 722 821 2261 2545 2899 722
Size: 99 1440 284 354 3234
Fragments arranged by size:
3234 1440 354 284 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1230 2245 2994 3127 3586 3795 1230
Size: 1015 749 133 459 209 2846
Fragments arranged by size:
2846 1015 749 459 209 133
TatI w'GTAC_w
Cuts at: 1623 4926 1623
Size: 3303 2108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1097 1129 1791 1823 4928 4960 1097
Size: 32 662 32 3105 32 1548
Fragments arranged by size:
3105 1548 662 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3208 3208
Size: 5411
XbaI T'CTAG_A
Cuts at: 2442 2442
Size: 5411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1050 2906 1050
Size: 1856 3555
XmaI C'CCGG_G
Cuts at: 2431 2431
Size: 5411
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI