Restriction map for
pF1KA0261
(Circular) (Six-base) MAPSORT of: pF1KA0261.seq Check: 662 from: 1 to: 6938
pF1KA0261 6938 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 242 242
Size: 6938
AccI GT'mk_AC
Cuts at: 3976 3976
Size: 6938
Acc65I G'GTAC_C
Cuts at: 3954 3954
Size: 6938
AclI AA'CG_TT
Cuts at: 6778 6778
Size: 6938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 842 3244 3673 4763 5195 5548 6210 677
Size: 165 2402 429 1090 432 353 662 1405
Fragments arranged by size:
2402 1405 1090 662 432 429 353 165
AfeI AGC'GCT
Cuts at: 3245 3245
Size: 6938
AflIII A'CryG_T
Cuts at: 2380 4290 6090 6371 2380
Size: 1910 1800 281 2947
Fragments arranged by size:
2947 1910 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1866 1866
Size: 6938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2250 2250
Size: 6938
AlwNI CAG_nnn'CTG
Cuts at: 2508 4243 5681 2508
Size: 1735 1438 3765
Fragments arranged by size:
3765 1735 1438
ApaI G_GGCC'C
Cuts at: 121 121
Size: 6938
ApaLI G'TGCA_C
Cuts at: 5776 5776
Size: 6938
ApoI r'AATT_y
Cuts at: 418 533 624 873 1290 1642 2302 3844 3942
Size: 115 91 249 417 352 660 1542 98
Cuts at: 3942 6291 6547 418
Size: 2349 256 809
Fragments arranged by size:
2349 1542 809 660 417 352 256 249
115 98 91
AseI AT'TA_AT
Cuts at: 20 850 20
Size: 830 6108
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6938
AvaI C'yCGr_G
Cuts at: 3958 3958
Size: 6938
BamHI G'GATC_C
Cuts at: 3963 3963
Size: 6938
BanI G'GyrC_C
Cuts at: 161 1312 3954 4368 4616 4651 161
Size: 1151 2642 414 248 35 2448
Fragments arranged by size:
2642 2448 1151 414 248 35
BanII G_rGCy'C
Cuts at: 121 3952 4982 121
Size: 3831 1030 2077
Fragments arranged by size:
3831 2077 1030
BbeI G_GCGC'C
Cuts at: 165 4372 4620 165
Size: 4207 248 2483
Fragments arranged by size:
4207 2483 248
BbsI GAAGACnn'nnnn_
Cuts at: 743 1115 2105 2252 743
Size: 372 990 147 5429
Fragments arranged by size:
5429 990 372 147
BbvCI CC'TCA_GC
Cuts at: 3065 3065
Size: 6938
BciVI GTATCCnnnnn_n'
Cuts at: 1479 4836 5892 6938 1479
Size: 3357 1056 1046 1479
Fragments arranged by size:
3357 1479 1056 1046
BclI T'GATC_A
Cuts at: 3533 4458 3533
Size: 925 6013
BglII A'GATC_T
Cuts at: 2586 4453 6490 2586
Size: 1867 2037 3034
Fragments arranged by size:
3034 2037 1867
BlpI GC'TnA_GC
Cuts at: 3081 4050 3081
Size: 969 5969
Bme1580I G_kGCm'C
Cuts at: 121 4563 4656 5780 121
Size: 4442 93 1124 1279
Fragments arranged by size:
4442 1279 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 996 2420 2744 4150 4231 4566 6710 996
Size: 1424 324 1406 81 335 2144 1224
Fragments arranged by size:
2144 1424 1406 1224 335 324 81
BmtI G_CTAG'C
Cuts at: 2582 3511 2582
Size: 929 6009
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5305 5305
Size: 6938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 590 116
Size: 474 6464
Bpu10I CC'TnA_GC
Cuts at: 454 1326 3065 3315 454
Size: 872 1739 250 4077
Fragments arranged by size:
4077 1739 872 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4114 4993 5707 6005 3505
Size: 609 879 714 298 4438
Fragments arranged by size:
4438 879 714 609 298
BsaI GGTCTCn'nnnn_
Cuts at: 466 3235 6642 466
Size: 2769 3407 762
Fragments arranged by size:
3407 2769 762
BsaAI yAC'GTr
Cuts at: 2277 4291 2277
Size: 2014 4924
BsaBI GATnn'nnATC
Cuts at: 6 1133 4452 6489 6
Size: 1127 3319 2037 455
Fragments arranged by size:
3319 2037 1127 455
BsaHI Gr'CG_yC
Cuts at: 162 4369 4617 5319 6815 162
Size: 4207 248 702 1496 285
Fragments arranged by size:
4207 1496 702 285 248
BsaWI w'CCGG_w
Cuts at: 4127 4648 5326 5737 5884 6158 4127
Size: 521 678 411 147 274 4907
Fragments arranged by size:
4907 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 353 3667 3670 353
Size: 3314 3 3621
Fragments arranged by size:
3621 3314 3
BseYI C'CCAG_C
Cuts at: 2492 3309 3503 4363 5121 5786 6194 2492
Size: 817 194 860 758 665 408 3236
Fragments arranged by size:
3236 860 817 758 665 408 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2210 3493 2144
Size: 66 1283 5589
Fragments arranged by size:
5589 1283 66
BsiEI CG_ry'CG
Cuts at: 104 4480 4526 5756 104
Size: 4376 46 1230 1286
Fragments arranged by size:
4376 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 802 2365 2451 3351 3391 3952 4730 4920 5780
Size: 1563 86 900 40 561 778 190 860
Cuts at: 5780 802
Size: 1960
Fragments arranged by size:
1960 1563 900 860 778 561 190 86
40
BsmI GAATG_Cn'
Cuts at: 2441 2441
Size: 6938
BsmBI CGTCTCn'nnnn_
Cuts at: 1558 1558
Size: 6938
Bsp1286I G_dGCh'C
Cuts at: 121 802 2365 2451 3351 3391 3952 4563 4656
Size: 681 1563 86 900 40 561 611 93
Cuts at: 4656 4730 4920 4982 5780 121
Size: 74 190 62 798 1279
Fragments arranged by size:
1563 1279 900 798 681 611 561 190
93 86 74 62 40
BspHI T'CATG_A
Cuts at: 879 1662 5370 879
Size: 783 3708 2447
Fragments arranged by size:
3708 2447 783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 3988 4202 4504 4885 242
Size: 3746 214 302 381 2295
Fragments arranged by size:
3746 2295 381 302 214
BsrBI CCG'CTC
Cuts at: 50 167 291 3495 5230 5284 6768 6933 50
Size: 117 124 3204 1735 54 1484 165 55
Fragments arranged by size:
3204 1735 1484 165 124 117 55 54
BsrDI GCAATG_nn'
Cuts at: 1851 2471 3927 4850 1851
Size: 620 1456 923 3939
Fragments arranged by size:
3939 1456 923 620
BsrFI r'CCGG_y
Cuts at: 149 237 3697 4936 5117 149
Size: 88 3460 1239 181 1970
Fragments arranged by size:
3460 1970 1239 181 88
BsrGI T'GTAC_A
Cuts at: 1212 2518 2697 1212
Size: 1306 179 5453
Fragments arranged by size:
5453 1306 179
BssSI C'ACGA_G
Cuts at: 5209 5917 5209
Size: 708 6230
BstAPI GCAn_nnn'nTGC
Cuts at: 1573 4315 1573
Size: 2742 4196
BstBI TT'CG_AA
Cuts at: 2433 5299 2433
Size: 2866 4072
BstEII G'GTnAC_C
Cuts at: 2006 2006
Size: 6938
BstXI CCAn_nnnn'nTGG
Cuts at: 483 483
Size: 6938
BstYI r'GATC_y
Cuts at: 1 573 1051 2565 2586 3963 4430 4453 4788
Size: 572 478 1514 21 1377 467 23 335
Cuts at: 4788 5034 5340 5352 5438 6490 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1514 1377 1052 572 478 467 449 335
306 246 86 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 3281 3281
Size: 6938
BtgI C'CryG_G
Cuts at: 107 275 311 107
Size: 168 36 6734
Fragments arranged by size:
6734 168 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4888 5069 5232 4888
Size: 181 163 6594
Fragments arranged by size:
6594 181 163
BtsI GCAGTG_nn'
Cuts at: 1514 3143 4181 1514
Size: 1629 1038 4271
Fragments arranged by size:
4271 1629 1038
ClaI AT'CG_AT
Cuts at: 1173 1173
Size: 6938
DraI TTT'AAA
Cuts at: 1167 2475 2796 3937 1167
Size: 1308 321 1141 4168
Fragments arranged by size:
4168 1308 1141 321
DrdI GACnn_nn'nnGTC
Cuts at: 4644 5988 4644
Size: 1344 5594
EaeI y'GGCC_r
Cuts at: 314 3888 4523 4697 5088 5115 6460 314
Size: 3574 635 174 391 27 1345 792
Fragments arranged by size:
3574 1345 792 635 391 174 27
EagI C'GGCC_G
Cuts at: 4523 4523
Size: 6938
EarI CTCTTCn'nnn_
Cuts at: 496 677 2879 3629 4961 5171 496
Size: 181 2202 750 1332 210 2263
Fragments arranged by size:
2263 2202 1332 750 210 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 168 315 5146 5888 6034 6748 168
Size: 147 4831 742 146 714 358
Fragments arranged by size:
4831 742 714 358 147 146
EcoICRI GAG'CTC
Cuts at: 3950 3950
Size: 6938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 590 677 842 3244 3673 4763 5195 5548
Size: 474 87 165 2402 429 1090 432 353
Cuts at: 5548 6210 116
Size: 662 844
Fragments arranged by size:
2402 1090 844 662 474 432 429 353
165 87
EcoNI CCTnn'n_nnAGG
Cuts at: 1366 3280 1366
Size: 1914 5024
EcoO109I rG'GnC_Cy
Cuts at: 117 118 4077 117
Size: 1 3959 2978
Fragments arranged by size:
3959 2978 1
EcoRI G'AATT_C
Cuts at: 3942 3942
Size: 6938
EcoRV GAT'ATC
Cuts at: 525 1098 525
Size: 573 6365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4957 4957
Size: 6938
FspI TGC'GCA
Cuts at: 4719 4719
Size: 6938
HaeII r_GCGC'y
Cuts at: 165 3247 4372 4620 5850 6621 165
Size: 3082 1125 248 1230 771 482
Fragments arranged by size:
3082 1230 1125 771 482 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 1087 1119 1852 1884 2535 2567 2611
Size: 32 469 32 733 32 651 32 44
Cuts at: 2611 2643 4780 4810 4812 4842 586
Size: 32 2137 30 2 30 2682
Fragments arranged by size:
2682 2137 733 651 469 44 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3372 3611 3977 3372
Size: 239 366 6333
Fragments arranged by size:
6333 366 239
KasI G'GCGC_C
Cuts at: 161 4368 4616 161
Size: 4207 248 2483
Fragments arranged by size:
4207 2483 248
KpnI G_GTAC'C
Cuts at: 3958 3958
Size: 6938
MluI A'CGCG_T
Cuts at: 6371 6371
Size: 6938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 461 659 1398 3858 5697 5881 461
Size: 198 739 2460 1839 184 1518
Fragments arranged by size:
2460 1839 1518 739 198 184
MscI TGG'CCA
Cuts at: 316 3890 4699 316
Size: 3574 809 2555
Fragments arranged by size:
3574 2555 809
MslI CAynn'nnrTG
Cuts at: 481 917 2232 3110 3166 4927 5054 481
Size: 436 1315 878 56 1761 127 2365
Fragments arranged by size:
2365 1761 1315 878 436 127 56
MspA1I CmG'CkG
Cuts at: 277 2524 4049 4363 4723 5507 5752 277
Size: 2247 1525 314 360 784 245 1463
Fragments arranged by size:
2247 1525 1463 784 360 314 245
NaeI GCC'GGC
Cuts at: 239 3699 5119 239
Size: 3460 1420 2058
Fragments arranged by size:
3460 2058 1420
NarI GG'CG_CC
Cuts at: 162 4369 4617 162
Size: 4207 248 2483
Fragments arranged by size:
4207 2483 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6938
NdeI CA'TA_TG
Cuts at: 3076 3076
Size: 6938
NgoMIV G'CCGG_C
Cuts at: 237 3697 5117 237
Size: 3460 1420 2058
Fragments arranged by size:
3460 2058 1420
NheI G'CTAG_C
Cuts at: 2578 3507 2578
Size: 929 6009
NspI r_CATG'y
Cuts at: 3697 3991 4491 6094 3697
Size: 294 500 1603 4541
Fragments arranged by size:
4541 1603 500 294
PciI A'CATG_T
Cuts at: 6090 6090
Size: 6938
PflMI CCAn_nnn'nTGG
Cuts at: 2039 2039
Size: 6938
PmeI GTTT'AAAC
Cuts at: 3937 3937
Size: 6938
PmlI CAC'GTG
Cuts at: 2277 2277
Size: 6938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5384 5416 3659
Size: 32 1693 32 5181
Fragments arranged by size:
5181 1693 32 32
PspOMI G'GGCC_C
Cuts at: 117 117
Size: 6938
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1205 1237 1205
Size: 32 6906
PstI C_TGCA'G
Cuts at: 1533 3985 4670 1533
Size: 2452 685 3801
Fragments arranged by size:
3801 2452 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6938
PvuII CAG'CTG
Cuts at: 2524 4363 4723 2524
Size: 1839 360 4739
Fragments arranged by size:
4739 1839 360
RsrII CG'GwC_CG
Cuts at: 5133 5133
Size: 6938
SacI G_AGCT'C
Cuts at: 3952 3952
Size: 6938
SacII CC_GC'GG
Cuts at: 278 278
Size: 6938
SalI G'TCGA_C
Cuts at: 3975 3975
Size: 6938
SapI GCTCTTCn'nnn_
Cuts at: 4961 5171 4961
Size: 210 6728
SbfI CC_TGCA'GG
Cuts at: 3985 3985
Size: 6938
ScaI AGT'ACT
Cuts at: 567 698 933 2341 6455 567
Size: 131 235 1408 4114 1050
Fragments arranged by size:
4114 1408 1050 235 131
SfcI C'TryA_G
Cuts at: 33 956 1239 1251 1529 3408 3981 4666 5634
Size: 923 283 12 278 1879 573 685 968
Cuts at: 5634 5825 33
Size: 191 1146
Fragments arranged by size:
1879 1146 968 923 685 573 283 278
191 12
SfoI GGC'GCC
Cuts at: 163 4370 4618 163
Size: 4207 248 2483
Fragments arranged by size:
4207 2483 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6938
SgrAI Cr'CCGG_yG
Cuts at: 149 149
Size: 6938
SmaI CCC'GGG
Cuts at: 3960 3960
Size: 6938
SmlI C'TyrA_G
Cuts at: 3484 4093 5008 5722 5984 3484
Size: 609 915 714 262 4438
Fragments arranged by size:
4438 915 714 609 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6938
SphI G_CATG'C
Cuts at: 3697 3991 4491 3697
Size: 294 500 6144
Fragments arranged by size:
6144 500 294
SspI AAT'ATT
Cuts at: 3919 3919
Size: 6938
StuI AGG'CCT
Cuts at: 1324 1324
Size: 6938
StyI C'CwwG_G
Cuts at: 107 1278 2480 4072 4426 107
Size: 1171 1202 1592 354 2619
Fragments arranged by size:
2619 1592 1202 1171 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2025 2806 3594 3740 4521 4654 5113 5322 2025
Size: 781 788 146 781 133 459 209 3641
Fragments arranged by size:
3641 788 781 781 459 209 146 133
TatI w'GTAC_w
Cuts at: 565 696 931 1212 2339 2518 2697 3053 6453
Size: 131 235 281 1127 179 179 356 3400
Cuts at: 6453 565
Size: 1050
Fragments arranged by size:
3400 1127 1050 356 281 235 179 179
131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3370 3402 6455 6487 3370
Size: 32 3053 32 3821
Fragments arranged by size:
3821 3053 32 32
Tth111I GACn'n_nGTC
Cuts at: 742 4735 742
Size: 3993 2945
XbaI T'CTAG_A
Cuts at: 2718 3904 3969 2718
Size: 1186 65 5687
Fragments arranged by size:
5687 1186 65
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4433 4433
Size: 6938
XmaI C'CCGG_G
Cuts at: 3958 3958
Size: 6938
XmnI GAAnn'nnTTC
Cuts at: 2284 3044 2284
Size: 760 6178
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PciI PflMI
PmeI PmlI PpiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AleI AloI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BsaXI BsiWI BspEI BssHII
BstZ17I CspCI DraIII FseI FspAI HindIII HpaI MfeI
NotI NruI NsiI PacI PasI PfoI PpuMI PshAI
PsiI PspXI SanDI SexAI SfiI SnaBI SrfI SwaI
XhoI ZraI