Restriction map for
pF1KA0259
(Circular) (Six-base) MAPSORT of: pF1KA0259.seq Check: 6508 from: 1 to: 7679
pF1KA0259 7679 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 1627 2661 1627
Size: 1034 6645
AccI GT'mk_AC
Cuts at: 512 3144 3215 4563 4717 512
Size: 2632 71 1348 154 3474
Fragments arranged by size:
3474 2632 1348 154 71
Acc65I G'GTAC_C
Cuts at: 4695 4695
Size: 7679
AclI AA'CG_TT
Cuts at: 7519 7519
Size: 7679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 208 613 1193 1442 1649 1772 2527 3610
Size: 68 405 580 249 207 123 755 1083
Cuts at: 3610 3752 3776 4336 5504 5936 6289 6951 140
Size: 142 24 560 1168 432 353 662 868
Fragments arranged by size:
1168 1083 868 755 662 580 560 432
405 353 249 207 142 123 68 24
AflIII A'CryG_T
Cuts at: 3723 4311 4487 4671 5031 6831 7112 3723
Size: 588 176 184 360 1800 281 4290
Fragments arranged by size:
4290 1800 588 360 281 184 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2431 2431
Size: 7679
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1250 1282 1418 1450 2114 2146 1250
Size: 32 136 32 664 32 6783
Fragments arranged by size:
6783 664 136 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2392 3499 4474 4984 6422 2392
Size: 1107 975 510 1438 3649
Fragments arranged by size:
3649 1438 1107 975 510
ApaLI G'TGCA_C
Cuts at: 6517 6517
Size: 7679
ApoI r'AATT_y
Cuts at: 202 1460 2241 2325 2382 2581 4683 7032 7288
Size: 1258 781 84 57 199 2102 2349 256
Cuts at: 7288 202
Size: 593
Fragments arranged by size:
2349 2102 1258 781 593 256 199 84
57
AseI AT'TA_AT
Cuts at: 20 20
Size: 7679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7679
AvaI C'yCGr_G
Cuts at: 187 4325 4699 187
Size: 4138 374 3167
Fragments arranged by size:
4138 3167 374
BamHI G'GATC_C
Cuts at: 4704 4704
Size: 7679
BanI G'GyrC_C
Cuts at: 4695 5109 5357 5392 4695
Size: 414 248 35 6982
Fragments arranged by size:
6982 414 248 35
BanII G_rGCy'C
Cuts at: 1667 2555 4693 5723 1667
Size: 888 2138 1030 3623
Fragments arranged by size:
3623 2138 1030 888
BbeI G_GCGC'C
Cuts at: 5113 5361 5113
Size: 248 7431
BbsI GAAGACnn'nnnn_
Cuts at: 146 1248 1703 3155 4154 4218 146
Size: 1102 455 1452 999 64 3607
Fragments arranged by size:
3607 1452 1102 999 455 64
BciVI GTATCCnnnnn_n'
Cuts at: 2562 5577 6633 7679 2562
Size: 3015 1056 1046 2562
Fragments arranged by size:
3015 2562 1056 1046
BclI T'GATC_A
Cuts at: 312 5199 312
Size: 4887 2792
BglI GCCn_nnn'nGGC
Cuts at: 4072 4072
Size: 7679
BglII A'GATC_T
Cuts at: 1572 5194 7231 1572
Size: 3622 2037 2020
Fragments arranged by size:
3622 2037 2020
BlpI GC'TnA_GC
Cuts at: 1661 4791 1661
Size: 3130 4549
Bme1580I G_kGCm'C
Cuts at: 3042 5304 5397 6521 3042
Size: 2262 93 1124 4200
Fragments arranged by size:
4200 2262 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2784 3806 4891 4972 5307 7451 2784
Size: 1022 1085 81 335 2144 3012
Fragments arranged by size:
3012 2144 1085 1022 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 4649 6046 3500
Size: 1149 1397 5133
Fragments arranged by size:
5133 1397 1149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2099 2636 1138
Size: 961 537 6181
Fragments arranged by size:
6181 961 537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 3788 3923 4518 4855 5734 6448 6746 335
Size: 3453 135 595 337 879 714 298 1268
Fragments arranged by size:
3453 1268 879 714 595 337 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 512 2496 7383 512
Size: 1984 4887 808
Fragments arranged by size:
4887 1984 808
BsaAI yAC'GTr
Cuts at: 3724 5032 3724
Size: 1308 6371
BsaBI GATnn'nnATC
Cuts at: 6 1571 5193 7230 6
Size: 1565 3622 2037 455
Fragments arranged by size:
3622 2037 1565 455
BsaHI Gr'CG_yC
Cuts at: 1624 2658 5110 5358 6060 7556 1624
Size: 1034 2452 248 702 1496 1747
Fragments arranged by size:
2452 1747 1496 1034 702 248
BsaWI w'CCGG_w
Cuts at: 4868 5389 6067 6478 6625 6899 4868
Size: 521 678 411 147 274 5648
Fragments arranged by size:
5648 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1250 1280 1922 1952 2114 2144 2542 2572 3328
Size: 30 642 30 162 30 398 30 756
Cuts at: 3328 3358 3720 3750 4355 4385 1250
Size: 30 362 30 605 30 4544
Fragments arranged by size:
4544 756 642 605 398 362 162 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3014 3577 3895 4376 4532 3014
Size: 563 318 481 156 6161
Fragments arranged by size:
6161 563 481 318 156
BseYI C'CCAG_C
Cuts at: 1843 1902 2523 2662 4078 5104 5862 6527 6935
Size: 59 621 139 1416 1026 758 665 408
Cuts at: 6935 1843
Size: 2587
Fragments arranged by size:
2587 1416 1026 758 665 621 408 139
59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 4159
Size: 7679
BsiEI CG_ry'CG
Cuts at: 104 5221 5267 6497 104
Size: 5117 46 1230 1286
Fragments arranged by size:
5117 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3023 4693 5471 5661 6521 3023
Size: 1670 778 190 860 4181
Fragments arranged by size:
4181 1670 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1667 2555 3023 3042 4693 5304 5397 5471 5661
Size: 888 468 19 1651 611 93 74 190
Cuts at: 5661 5723 6521 1667
Size: 62 798 2825
Fragments arranged by size:
2825 1651 888 798 611 468 190 93
74 62 19
BspHI T'CATG_A
Cuts at: 3888 6111 3888
Size: 2223 5456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2256 4729 4943 5245 5626 2256
Size: 2473 214 302 381 4309
Fragments arranged by size:
4309 2473 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5971 6025 7509 7674 50
Size: 5921 54 1484 165 55
Fragments arranged by size:
5921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4235 4511 5591 4235
Size: 276 1080 6323
Fragments arranged by size:
6323 1080 276
BsrFI r'CCGG_y
Cuts at: 3128 5677 5858 3128
Size: 2549 181 4949
Fragments arranged by size:
4949 2549 181
BsrGI T'GTAC_A
Cuts at: 445 489 820 882 4018 4200 445
Size: 44 331 62 3136 182 3924
Fragments arranged by size:
3924 3136 331 182 62 44
BssSI C'ACGA_G
Cuts at: 5950 6658 5950
Size: 708 6971
BstAPI GCAn_nnn'nTGC
Cuts at: 3577 4474 5056 3577
Size: 897 582 6200
Fragments arranged by size:
6200 897 582
BstBI TT'CG_AA
Cuts at: 4047 6040 4047
Size: 1993 5686
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2077
Size: 7679
BstYI r'GATC_y
Cuts at: 1 1572 3186 4704 5171 5194 5529 5775 6081
Size: 1571 1614 1518 467 23 335 246 306
Cuts at: 6081 6093 6179 7231 1
Size: 12 86 1052 449
Fragments arranged by size:
1614 1571 1518 1052 467 449 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 513 513
Size: 7679
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 396 1803 4081 5629 5810 5973 396
Size: 1407 2278 1548 181 163 2102
Fragments arranged by size:
2278 2102 1548 1407 181 163
BtsI GCAGTG_nn'
Cuts at: 3122 3453 3598 4006 4922 3122
Size: 331 145 408 916 5879
Fragments arranged by size:
5879 916 408 331 145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1593 1628 1593
Size: 35 7644
DraI TTT'AAA
Cuts at: 149 180 1502 4401 4678 149
Size: 31 1322 2899 277 3150
Fragments arranged by size:
3150 2899 1322 277 31
DraIII CAC_nnn'GTG
Cuts at: 891 902 4137 891
Size: 11 3235 4433
Fragments arranged by size:
4433 3235 11
DrdI GACnn_nn'nnGTC
Cuts at: 5385 6729 5385
Size: 1344 6335
EaeI y'GGCC_r
Cuts at: 2956 3126 3224 5264 5438 5829 5856 7201 2956
Size: 170 98 2040 174 391 27 1345 3434
Fragments arranged by size:
3434 2040 1345 391 174 170 98 27
EagI C'GGCC_G
Cuts at: 5264 5264
Size: 7679
EarI CTCTTCn'nnn_
Cuts at: 236 2011 2608 5702 5912 236
Size: 1775 597 3094 210 2003
Fragments arranged by size:
3094 2003 1775 597 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 518 5887 6629 6775 7489 518
Size: 5369 742 146 714 708
Fragments arranged by size:
5369 742 714 708 146
EcoICRI GAG'CTC
Cuts at: 4691 4691
Size: 7679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 208 613 1138 1193 1442 1649 1772 2099
Size: 68 405 525 55 249 207 123 327
Cuts at: 2099 2527 2636 3610 3752 3776 4336 5504 5936
Size: 428 109 974 142 24 560 1168 432
Cuts at: 5936 6289 6951 140
Size: 353 662 868
Fragments arranged by size:
1168 974 868 662 560 525 432 428
405 353 327 249 207 142 123 109
68 55 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2590 2590
Size: 7679
EcoO109I rG'GnC_Cy
Cuts at: 1637 4818 1637
Size: 3181 4498
EcoRI G'AATT_C
Cuts at: 202 4683 202
Size: 4481 3198
EcoRV GAT'ATC
Cuts at: 3108 3108
Size: 7679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 238 1513 1551 2631 4020 5698 238
Size: 1275 38 1080 1389 1678 2219
Fragments arranged by size:
2219 1678 1389 1275 1080 38
FspI TGC'GCA
Cuts at: 5460 5460
Size: 7679
HaeII r_GCGC'y
Cuts at: 5113 5361 6591 7362 5113
Size: 248 1230 771 5430
Fragments arranged by size:
5430 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1564 1596 1978 2010 2560 2592 2776 2808 2968
Size: 32 382 32 550 32 184 32 160
Cuts at: 2968 3000 3328 3360 3720 3752 5521 5551 5553
Size: 32 328 32 360 32 1769 30 2
Cuts at: 5553 5583 1564
Size: 30 3660
Fragments arranged by size:
3660 1769 550 382 360 328 184 160
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3145 3507 3633 4718 3145
Size: 362 126 1085 6106
Fragments arranged by size:
6106 1085 362 126
HindIII A'AGCT_T
Cuts at: 325 2474 325
Size: 2149 5530
KasI G'GCGC_C
Cuts at: 5109 5357 5109
Size: 248 7431
KpnI G_GTAC'C
Cuts at: 4699 4699
Size: 7679
MfeI C'AATT_G
Cuts at: 802 1022 802
Size: 220 7459
MluI A'CGCG_T
Cuts at: 7112 7112
Size: 7679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1174 2252 6438 6622 1085
Size: 89 1078 4186 184 2142
Fragments arranged by size:
4186 2142 1078 184 89
MscI TGG'CCA
Cuts at: 3226 5440 3226
Size: 2214 5465
MslI CAynn'nnrTG
Cuts at: 485 3724 4030 5668 5795 485
Size: 3239 306 1638 127 2369
Fragments arranged by size:
3239 2369 1638 306 127
MspA1I CmG'CkG
Cuts at: 3157 4078 4790 5104 5464 6248 6493 3157
Size: 921 712 314 360 784 245 4343
Fragments arranged by size:
4343 921 784 712 360 314 245
NaeI GCC'GGC
Cuts at: 3130 5860 3130
Size: 2730 4949
NarI GG'CG_CC
Cuts at: 5110 5358 5110
Size: 248 7431
NgoMIV G'CCGG_C
Cuts at: 3128 5858 3128
Size: 2730 4949
NspI r_CATG'y
Cuts at: 2175 2503 4315 4732 5232 6835 2175
Size: 328 1812 417 500 1603 3019
Fragments arranged by size:
3019 1812 1603 500 417 328
PciI A'CATG_T
Cuts at: 4311 6831 4311
Size: 2520 5159
PflMI CCAn_nnn'nTGG
Cuts at: 875 902 1455 3256 875
Size: 27 553 1801 5298
Fragments arranged by size:
5298 1801 553 27
PfoI T'CCnGG_A
Cuts at: 2652 3711 2652
Size: 1059 6620
PmeI GTTT'AAAC
Cuts at: 4678 4678
Size: 7679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1250 1282 2114 2146 6125 6157 1250
Size: 32 832 32 3979 32 2772
Fragments arranged by size:
3979 2772 832 32 32 32
PshAI GACnn'nnGTC
Cuts at: 3956 3956
Size: 7679
PsiI TTA'TAA
Cuts at: 413 3086 3871 413
Size: 2673 785 4221
Fragments arranged by size:
4221 2673 785
PstI C_TGCA'G
Cuts at: 337 2397 4120 4726 5411 337
Size: 2060 1723 606 685 2605
Fragments arranged by size:
2605 2060 1723 685 606
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7679
PvuII CAG'CTG
Cuts at: 3157 4078 5104 5464 3157
Size: 921 1026 360 5372
Fragments arranged by size:
5372 1026 921 360
RsrII CG'GwC_CG
Cuts at: 5874 5874
Size: 7679
SacI G_AGCT'C
Cuts at: 4693 4693
Size: 7679
SalI G'TCGA_C
Cuts at: 3143 4716 3143
Size: 1573 6106
SapI GCTCTTCn'nnn_
Cuts at: 5702 5912 5702
Size: 210 7469
SbfI CC_TGCA'GG
Cuts at: 4120 4726 4120
Size: 606 7073
ScaI AGT'ACT
Cuts at: 2184 7196 2184
Size: 5012 2667
SexAI A'CCwGG_T
Cuts at: 4377 4377
Size: 7679
SfcI C'TryA_G
Cuts at: 33 333 2258 2393 3551 4116 4637 4722 5407
Size: 300 1925 135 1158 565 521 85 685
Cuts at: 5407 6375 6566 33
Size: 968 191 1146
Fragments arranged by size:
1925 1158 1146 968 685 565 521 300
191 135 85
SfoI GGC'GCC
Cuts at: 5111 5359 5111
Size: 248 7431
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7679
SmaI CCC'GGG
Cuts at: 4701 4701
Size: 7679
SmlI C'TyrA_G
Cuts at: 187 350 3803 3938 4325 4497 4834 5749 6463
Size: 163 3453 135 387 172 337 915 714
Cuts at: 6463 6725 187
Size: 262 1141
Fragments arranged by size:
3453 1141 915 714 387 337 262 172
163 135
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7679
SphI G_CATG'C
Cuts at: 4732 5232 4732
Size: 500 7179
SspI AAT'ATT
Cuts at: 574 1056 574
Size: 482 7197
StuI AGG'CCT
Cuts at: 1362 1362
Size: 7679
StyI C'CwwG_G
Cuts at: 2118 2591 2642 3369 4813 5167 2118
Size: 473 51 727 1444 354 4630
Fragments arranged by size:
4630 1444 727 473 354 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3761 4077 4283 5262 5395 5854 6063 3761
Size: 316 206 979 133 459 209 5377
Fragments arranged by size:
5377 979 459 316 209 206 133
TatI w'GTAC_w
Cuts at: 445 489 820 882 2182 3003 3908 4018 4200
Size: 44 331 62 1300 821 905 110 182
Cuts at: 4200 4490 4668 7194 445
Size: 290 178 2526 930
Fragments arranged by size:
2526 1300 930 905 821 331 290 182
178 110 62 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2001 2033 2998 3030 4121 4153 4355 4387 7196
Size: 32 965 32 1091 32 202 32 2809
Cuts at: 7196 7228 2001
Size: 32 2452
Fragments arranged by size:
2809 2452 1091 965 202 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5476 5476
Size: 7679
XbaI T'CTAG_A
Cuts at: 1137 4710 1137
Size: 3573 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3099 3840 5174 3099
Size: 741 1334 5604
Fragments arranged by size:
5604 1334 741
XhoI C'TCGA_G
Cuts at: 187 4325 187
Size: 4138 3541
XmaI C'CCGG_G
Cuts at: 4699 4699
Size: 7679
XmnI GAAnn'nnTTC
Cuts at: 221 476 4167 221
Size: 255 3691 3733
Fragments arranged by size:
3733 3691 255
ZraI GAC'GTC
Cuts at: 1625 2659 1625
Size: 1034 6645
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BspEI BssHII BstEII Bsu36I BtgI
ClaI FseI FspAI HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI