Restriction map for
pF1KA0255
(Circular) (Six-base) MAPSORT of: pF1KA0255.seq Check: 6807 from: 1 to: 4988
pF1KA0255 4988 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AatII G_ACGT'C
Cuts at: 884 1058 884
Size: 174 4814
AccI GT'mk_AC
Cuts at: 1448 2026 1448
Size: 578 4410
Acc65I G'GTAC_C
Cuts at: 751 1918 2004 751
Size: 1167 86 3735
Fragments arranged by size:
3735 1167 86
AclI AA'CG_TT
Cuts at: 4828 4828
Size: 4988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1044 1605 2813 3245 3598 4260 1044
Size: 561 1208 432 353 662 1772
Fragments arranged by size:
1772 1208 662 561 432 353
AflIII A'CryG_T
Cuts at: 656 1943 2340 4140 4421 656
Size: 1287 397 1800 281 1223
Fragments arranged by size:
1800 1287 1223 397 281
AleI CACnn'nnGTG
Cuts at: 1402 1402
Size: 4988
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1627 1659 1627
Size: 32 4956
AlwNI CAG_nnn'CTG
Cuts at: 2293 3731 2293
Size: 1438 3550
ApaLI G'TGCA_C
Cuts at: 735 1045 3826 735
Size: 310 2781 1897
Fragments arranged by size:
2781 1897 310
ApoI r'AATT_y
Cuts at: 1767 1992 4341 4597 1767
Size: 225 2349 256 2158
Fragments arranged by size:
2349 2158 256 225
AseI AT'TA_AT
Cuts at: 20 20
Size: 4988
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
AvaI C'yCGr_G
Cuts at: 1528 2008 1528
Size: 480 4508
BamHI G'GATC_C
Cuts at: 2013 2013
Size: 4988
BanI G'GyrC_C
Cuts at: 751 1386 1591 1918 2004 2418 2666 2701 751
Size: 635 205 327 86 414 248 35 3038
Fragments arranged by size:
3038 635 414 327 248 205 86 35
BanII G_rGCy'C
Cuts at: 494 1105 1188 1610 1789 2002 3032 494
Size: 611 83 422 179 213 1030 2450
Fragments arranged by size:
2450 1030 611 422 213 179 83
BbeI G_GCGC'C
Cuts at: 1595 2422 2670 1595
Size: 827 248 3913
Fragments arranged by size:
3913 827 248
BbsI GAAGACnn'nnnn_
Cuts at: 443 918 1019 1050 443
Size: 475 101 31 4381
Fragments arranged by size:
4381 475 101 31
BbvCI CC'TCA_GC
Cuts at: 1087 1087
Size: 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1323 1357 1407 1441 1323
Size: 34 50 34 4870
Fragments arranged by size:
4870 50 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 952 1704 2886 3942 4988 952
Size: 752 1182 1056 1046 952
Fragments arranged by size:
1182 1056 1046 952 752
BclI T'GATC_A
Cuts at: 2508 2508
Size: 4988
BglII A'GATC_T
Cuts at: 584 1958 2503 4540 584
Size: 1374 545 2037 1032
Fragments arranged by size:
2037 1374 1032 545
BlpI GC'TnA_GC
Cuts at: 2100 2100
Size: 4988
Bme1580I G_kGCm'C
Cuts at: 739 1049 1391 2613 2706 3830 739
Size: 310 342 1222 93 1124 1897
Fragments arranged by size:
1897 1222 1124 342 310 93
BmrI ACTGGGnnnn_n'
Cuts at: 831 1065 2200 2281 2616 4760 831
Size: 234 1135 81 335 2144 1059
Fragments arranged by size:
2144 1135 1059 335 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 513 3355 513
Size: 2842 2146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 724 1158 508
Size: 216 434 4338
Fragments arranged by size:
4338 434 216
Bpu10I CC'TnA_GC
Cuts at: 946 1087 1104 946
Size: 141 17 4830
Fragments arranged by size:
4830 141 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2164 3043 3757 4055 2164
Size: 879 714 298 3097
Fragments arranged by size:
3097 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4692 4692
Size: 4988
BsaAI yAC'GTr
Cuts at: 659 2341 659
Size: 1682 3306
BsaBI GATnn'nnATC
Cuts at: 6 1132 2502 4539 6
Size: 1126 1370 2037 455
Fragments arranged by size:
2037 1370 1126 455
BsaHI Gr'CG_yC
Cuts at: 881 1055 1592 2419 2667 3369 4865 881
Size: 174 537 827 248 702 1496 1004
Fragments arranged by size:
1496 1004 827 702 537 248 174
BsaWI w'CCGG_w
Cuts at: 305 971 2177 2698 3376 3787 3934 4208 305
Size: 666 1206 521 678 411 147 274 1085
Fragments arranged by size:
1206 1085 678 666 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 643 673 1018 1048 1627 1657 643
Size: 30 345 30 579 30 3974
Fragments arranged by size:
3974 579 345 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 750 1039 1855 750
Size: 289 816 3883
Fragments arranged by size:
3883 816 289
BseYI C'CCAG_C
Cuts at: 212 253 854 1098 1573 2413 3171 3836 4244
Size: 41 601 244 475 840 758 665 408
Cuts at: 4244 212
Size: 956
Fragments arranged by size:
956 840 758 665 601 475 408 244
41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 559 577 1760 387
Size: 172 18 1183 3615
Fragments arranged by size:
3615 1183 172 18
BsiEI CG_ry'CG
Cuts at: 104 2530 2576 3806 104
Size: 2426 46 1230 1286
Fragments arranged by size:
2426 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 494 656 739 1049 1188 1610 2002 2780 2970
Size: 162 83 310 139 422 392 778 190
Cuts at: 2970 3830 494
Size: 860 1652
Fragments arranged by size:
1652 860 778 422 392 310 190 162
139 83
BsmI GAATG_Cn'
Cuts at: 1109 1792 1109
Size: 683 4305
Bsp1286I G_dGCh'C
Cuts at: 494 656 739 1049 1105 1188 1391 1610 1789
Size: 162 83 310 56 83 203 219 179
Cuts at: 1789 2002 2613 2706 2780 2970 3032 3830 494
Size: 213 611 93 74 190 62 798 1652
Fragments arranged by size:
1652 798 611 310 219 213 203 190
179 162 93 83 83 74 62 56
BspEI T'CCGG_A
Cuts at: 971 971
Size: 4988
BspHI T'CATG_A
Cuts at: 335 1190 3420 335
Size: 855 2230 1903
Fragments arranged by size:
2230 1903 855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 476 599 2038 2252 2554 2935 476
Size: 123 1439 214 302 381 2529
Fragments arranged by size:
2529 1439 381 302 214 123
BsrBI CCG'CTC
Cuts at: 50 426 1384 3280 3334 4818 4983 50
Size: 376 958 1896 54 1484 165 55
Fragments arranged by size:
1896 1484 958 376 165 55 54
BsrDI GCAATG_nn'
Cuts at: 455 980 2900 455
Size: 525 1920 2543
Fragments arranged by size:
2543 1920 525
BsrFI r'CCGG_y
Cuts at: 557 2986 3167 557
Size: 2429 181 2378
Fragments arranged by size:
2429 2378 181
BssSI C'ACGA_G
Cuts at: 415 3259 3967 415
Size: 2844 708 1436
Fragments arranged by size:
2844 1436 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2365 2365
Size: 4988
BstBI TT'CG_AA
Cuts at: 3349 3349
Size: 4988
BstXI CCAn_nnnn'nTGG
Cuts at: 486 1404 486
Size: 918 4070
BstYI r'GATC_y
Cuts at: 1 584 887 1430 1523 1577 1958 2013 2480
Size: 583 303 543 93 54 381 55 467
Cuts at: 2480 2503 2838 3084 3390 3402 3488 4540 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 583 543 467 449 381 335 306
303 246 93 86 55 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 746 746
Size: 4988
BtgI C'CryG_G
Cuts at: 1019 1400 1019
Size: 381 4607
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 527 2938 3119 3282 527
Size: 2411 181 163 2233
Fragments arranged by size:
2411 2233 181 163
BtsI GCAGTG_nn'
Cuts at: 235 2231 235
Size: 1996 2992
DraI TTT'AAA
Cuts at: 1268 1987 1268
Size: 719 4269
DrdI GACnn_nn'nnGTC
Cuts at: 310 445 2694 4038 310
Size: 135 2249 1344 1260
Fragments arranged by size:
2249 1344 1260 135
EaeI y'GGCC_r
Cuts at: 419 476 1248 2573 2747 3138 3165 4510 419
Size: 57 772 1325 174 391 27 1345 897
Fragments arranged by size:
1345 1325 897 772 391 174 57 27
EagI C'GGCC_G
Cuts at: 2573 2573
Size: 4988
EarI CTCTTCn'nnn_
Cuts at: 635 721 1216 1615 3011 3221 635
Size: 86 495 399 1396 210 2402
Fragments arranged by size:
2402 1396 495 399 210 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1250 3196 3938 4084 4798 1250
Size: 1946 742 146 714 1440
Fragments arranged by size:
1946 1440 742 714 146
EcoICRI GAG'CTC
Cuts at: 492 1186 1608 2000 492
Size: 694 422 392 3480
Fragments arranged by size:
3480 694 422 392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 724 1044 1158 1605 2813 3245 3598 4260
Size: 216 320 114 447 1208 432 353 662
Cuts at: 4260 508
Size: 1236
Fragments arranged by size:
1236 1208 662 447 432 353 320 216
114
EcoNI CCTnn'n_nnAGG
Cuts at: 973 973
Size: 4988
EcoO109I rG'GnC_Cy
Cuts at: 708 1064 2127 708
Size: 356 1063 3569
Fragments arranged by size:
3569 1063 356
EcoRI G'AATT_C
Cuts at: 1992 1992
Size: 4988
EcoRV GAT'ATC
Cuts at: 837 837
Size: 4988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4988
FspI TGC'GCA
Cuts at: 1501 2769 1501
Size: 1268 3720
FspAI rTGC'GCAy
Cuts at: 1501 1501
Size: 4988
HaeII r_GCGC'y
Cuts at: 125 1595 2422 2670 3900 4671 125
Size: 1470 827 248 1230 771 442
Fragments arranged by size:
1470 1230 827 771 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2830 2860 2862 2892 2830
Size: 30 2 30 4926
Fragments arranged by size:
4926 30 30 2
HincII GTy'rAC
Cuts at: 315 579 1830 2027 315
Size: 264 1251 197 3276
Fragments arranged by size:
3276 1251 264 197
HpaI GTT'AAC
Cuts at: 1830 1830
Size: 4988
KasI G'GCGC_C
Cuts at: 1591 2418 2666 1591
Size: 827 248 3913
Fragments arranged by size:
3913 827 248
KpnI G_GTAC'C
Cuts at: 755 1922 2008 755
Size: 1167 86 3735
Fragments arranged by size:
3735 1167 86
MluI A'CGCG_T
Cuts at: 4421 4421
Size: 4988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 524 3747 3931 401
Size: 123 3223 184 1458
Fragments arranged by size:
3223 1458 184 123
MscI TGG'CCA
Cuts at: 478 2749 478
Size: 2271 2717
MslI CAynn'nnrTG
Cuts at: 1219 1225 1402 1567 1687 1717 2977 3104 1219
Size: 6 177 165 120 30 1260 127 3103
Fragments arranged by size:
3103 1260 177 165 127 120 30 6
MspA1I CmG'CkG
Cuts at: 798 1116 1536 1734 1754 2099 2413 2773 3557
Size: 318 420 198 20 345 314 360 784
Cuts at: 3557 3802 798
Size: 245 1984
Fragments arranged by size:
1984 784 420 360 345 318 314 245
198 20
NaeI GCC'GGC
Cuts at: 3169 3169
Size: 4988
NarI GG'CG_CC
Cuts at: 1592 2419 2667 1592
Size: 827 248 3913
Fragments arranged by size:
3913 827 248
NcoI C'CATG_G
Cuts at: 1019 1400 1019
Size: 381 4607
NdeI CA'TA_TG
Cuts at: 1485 1485
Size: 4988
NgoMIV G'CCGG_C
Cuts at: 3167 3167
Size: 4988
NspI r_CATG'y
Cuts at: 1422 1947 2041 2541 4144 1422
Size: 525 94 500 1603 2266
Fragments arranged by size:
2266 1603 525 500 94
PciI A'CATG_T
Cuts at: 1943 4140 1943
Size: 2197 2791
PmeI GTTT'AAAC
Cuts at: 1987 1987
Size: 4988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1627 1659 3434 3466 1627
Size: 32 1775 32 3149
Fragments arranged by size:
3149 1775 32 32
PpuMI rG'GwC_Cy
Cuts at: 708 708
Size: 4988
PstI C_TGCA'G
Cuts at: 2035 2720 2035
Size: 685 4303
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4988
PvuII CAG'CTG
Cuts at: 798 1116 1734 2413 2773 798
Size: 318 618 679 360 3013
Fragments arranged by size:
3013 679 618 360 318
RsrII CG'GwC_CG
Cuts at: 3183 3183
Size: 4988
SacI G_AGCT'C
Cuts at: 494 1188 1610 2002 494
Size: 694 422 392 3480
Fragments arranged by size:
3480 694 422 392
SalI G'TCGA_C
Cuts at: 2025 2025
Size: 4988
SapI GCTCTTCn'nnn_
Cuts at: 1615 3011 3221 1615
Size: 1396 210 3382
Fragments arranged by size:
3382 1396 210
SbfI CC_TGCA'GG
Cuts at: 2035 2035
Size: 4988
ScaI AGT'ACT
Cuts at: 4505 4505
Size: 4988
SfcI C'TryA_G
Cuts at: 33 2031 2716 3684 3875 33
Size: 1998 685 968 191 1146
Fragments arranged by size:
1998 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1593 2420 2668 1593
Size: 827 248 3913
Fragments arranged by size:
3913 827 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
SmaI CCC'GGG
Cuts at: 2010 2010
Size: 4988
SmlI C'TyrA_G
Cuts at: 2143 3058 3772 4034 2143
Size: 915 714 262 3097
Fragments arranged by size:
3097 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4988
SphI G_CATG'C
Cuts at: 2041 2541 2041
Size: 500 4488
StyI C'CwwG_G
Cuts at: 785 1019 1400 2122 2476 785
Size: 234 381 722 354 3297
Fragments arranged by size:
3297 722 381 354 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1022 2571 2704 3163 3372 1022
Size: 1549 133 459 209 2638
Fragments arranged by size:
2638 1549 459 209 133
TatI w'GTAC_w
Cuts at: 800 1724 4503 800
Size: 924 2779 1285
Fragments arranged by size:
2779 1285 924
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1018 1050 4505 4537 1018
Size: 32 3455 32 1469
Fragments arranged by size:
3455 1469 32 32
Tth111I GACn'n_nGTC
Cuts at: 2785 2785
Size: 4988
XbaI T'CTAG_A
Cuts at: 2019 2019
Size: 4988
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 2483
Size: 4988
XmaI C'CCGG_G
Cuts at: 2008 2008
Size: 4988
XmnI GAAnn'nnTTC
Cuts at: 1354 1642 1354
Size: 288 4700
ZraI GAC'GTC
Cuts at: 882 1056 882
Size: 174 4814
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII FseI
HindIII MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI