Restriction map for
pF1KA0254
(Circular) (Six-base) MAPSORT of: pF1KA0254.seq Check: 3210 from: 1 to: 6089
pF1KA0254 6089 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 611 611
Size: 6089
AccI GT'mk_AC
Cuts at: 333 3127 333
Size: 2794 3295
Acc65I G'GTAC_C
Cuts at: 458 3105 458
Size: 2647 3442
AclI AA'CG_TT
Cuts at: 5929 5929
Size: 6089
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 749 1385 1638 2345 2731 3914 4346 4699
Size: 438 636 253 707 386 1183 432 353
Cuts at: 4699 5361 311
Size: 662 1039
Fragments arranged by size:
1183 1039 707 662 636 438 432 386
353 253
AfeI AGC'GCT
Cuts at: 2722 2722
Size: 6089
AflIII A'CryG_T
Cuts at: 248 742 2487 3441 5241 5522 248
Size: 494 1745 954 1800 281 815
Fragments arranged by size:
1800 1745 954 815 494 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1834 2045 1834
Size: 211 5878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 338 370 338
Size: 32 6057
AlwNI CAG_nnn'CTG
Cuts at: 877 1338 1378 1827 2817 2847 3394 4832 877
Size: 461 40 449 990 30 547 1438 2134
Fragments arranged by size:
2134 1438 990 547 461 449 40 30
ApaLI G'TGCA_C
Cuts at: 712 4927 712
Size: 4215 1874
ApoI r'AATT_y
Cuts at: 756 1431 2300 2879 2989 3093 5442 5698 756
Size: 675 869 579 110 104 2349 256 1147
Fragments arranged by size:
2349 1147 869 675 579 256 110 104
AseI AT'TA_AT
Cuts at: 20 20
Size: 6089
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6089
AvaI C'yCGr_G
Cuts at: 1730 3109 1730
Size: 1379 4710
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1692 1725 1692
Size: 33 6056
BamHI G'GATC_C
Cuts at: 884 3114 884
Size: 2230 3859
BanI G'GyrC_C
Cuts at: 458 777 1717 3105 3519 3767 3802 458
Size: 319 940 1388 414 248 35 2745
Fragments arranged by size:
2745 1388 940 414 319 248 35
BanII G_rGCy'C
Cuts at: 307 1585 1667 2206 3103 4133 307
Size: 1278 82 539 897 1030 2263
Fragments arranged by size:
2263 1278 1030 897 539 82
BbeI G_GCGC'C
Cuts at: 3523 3771 3523
Size: 248 5841
BbsI GAAGACnn'nnnn_
Cuts at: 118 1220 2179 118
Size: 1102 959 4028
Fragments arranged by size:
4028 1102 959
BbvCI CC'TCA_GC
Cuts at: 992 1379 2281 992
Size: 387 902 4800
Fragments arranged by size:
4800 902 387
BciVI GTATCCnnnnn_n'
Cuts at: 3987 5043 6089 3987
Size: 1056 1046 3987
Fragments arranged by size:
3987 1056 1046
BclI T'GATC_A
Cuts at: 857 3609 857
Size: 2752 3337
BglII A'GATC_T
Cuts at: 1899 1950 2865 3604 5641 1899
Size: 51 915 739 2037 2347
Fragments arranged by size:
2347 2037 915 739 51
BlpI GC'TnA_GC
Cuts at: 1644 2905 3201 1644
Size: 1261 296 4532
Fragments arranged by size:
4532 1261 296
Bme1580I G_kGCm'C
Cuts at: 716 782 951 971 1320 3714 3807 4931 716
Size: 66 169 20 349 2394 93 1124 1874
Fragments arranged by size:
2394 1874 1124 349 169 93 66 20
BmgBI CAC'GTC
Cuts at: 2488 2488
Size: 6089
BmrI ACTGGGnnnn_n'
Cuts at: 716 1033 1255 3301 3382 3717 5861 716
Size: 317 222 2046 81 335 2144 944
Fragments arranged by size:
2144 2046 944 335 317 222 81
BmtI G_CTAG'C
Cuts at: 2270 2270
Size: 6089
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1040 4456 1040
Size: 3416 2673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 291 338 1010 1070 1246 1259 1534 1906
Size: 163 47 672 60 176 13 275 372
Cuts at: 1906 2291 2338 2944 128
Size: 385 47 606 3273
Fragments arranged by size:
3273 672 606 385 372 275 176 163
60 47 47 13
Bpu10I CC'TnA_GC
Cuts at: 992 1379 1669 2281 992
Size: 387 290 612 4800
Fragments arranged by size:
4800 612 387 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3265 4144 4858 5156 3265
Size: 879 714 298 4198
Fragments arranged by size:
4198 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1425 2130 2224 5793 1425
Size: 705 94 3569 1721
Fragments arranged by size:
3569 1721 705 94
BsaAI yAC'GTr
Cuts at: 3442 3442
Size: 6089
BsaBI GATnn'nnATC
Cuts at: 6 3603 5640 6
Size: 3597 2037 455
Fragments arranged by size:
3597 2037 455
BsaHI Gr'CG_yC
Cuts at: 810 3520 3768 4470 5966 810
Size: 2710 248 702 1496 933
Fragments arranged by size:
2710 1496 933 702 248
BsaWI w'CCGG_w
Cuts at: 805 3278 3799 4477 4888 5035 5309 805
Size: 2473 521 678 411 147 274 1585
Fragments arranged by size:
2473 1585 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 357 387 357
Size: 30 6059
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1396 1611 1903 2071 2227 2581 3031 3069 1396
Size: 215 292 168 156 354 450 38 4416
Fragments arranged by size:
4416 450 354 292 215 168 156 38
BseYI C'CCAG_C
Cuts at: 288 300 939 2533 2723 2909 3514 4272 4937
Size: 12 639 1594 190 186 605 758 665
Cuts at: 4937 5345 288
Size: 408 1032
Fragments arranged by size:
1594 1032 758 665 639 605 408 190
186 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2083 2713 2749 2083
Size: 630 36 5423
Fragments arranged by size:
5423 630 36
BsiEI CG_ry'CG
Cuts at: 104 1546 3631 3677 4907 104
Size: 1442 2085 46 1230 1286
Fragments arranged by size:
2085 1442 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 716 1585 1739 3103 3881 4071 4931 716
Size: 869 154 1364 778 190 860 1874
Fragments arranged by size:
1874 1364 869 860 778 190 154
Bsp1286I G_dGCh'C
Cuts at: 307 716 782 951 971 1320 1585 1667 1739
Size: 409 66 169 20 349 265 82 72
Cuts at: 1739 2206 3103 3714 3807 3881 4071 4133 4931
Size: 467 897 611 93 74 190 62 798
Cuts at: 4931 307
Size: 1465
Fragments arranged by size:
1465 897 798 611 467 409 349 265
190 169 93 82 74 72 66 62
20
BspHI T'CATG_A
Cuts at: 1268 4521 1268
Size: 3253 2836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 1540 2685 3139 3353 3655 4036 611
Size: 929 1145 454 214 302 381 2664
Fragments arranged by size:
2664 1145 929 454 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4381 4435 5919 6084 50
Size: 4331 54 1484 165 55
Fragments arranged by size:
4331 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 274 846 4001 274
Size: 572 3155 2362
Fragments arranged by size:
3155 2362 572
BsrFI r'CCGG_y
Cuts at: 1416 2900 4087 4268 1416
Size: 1484 1187 181 3237
Fragments arranged by size:
3237 1484 1187 181
BssSI C'ACGA_G
Cuts at: 898 4360 5068 898
Size: 3462 708 1919
Fragments arranged by size:
3462 1919 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3466 3466
Size: 6089
BstBI TT'CG_AA
Cuts at: 4450 4450
Size: 6089
BstXI CCAn_nnnn'nTGG
Cuts at: 2723 2723
Size: 6089
BstYI r'GATC_y
Cuts at: 1 873 884 924 1445 1563 1899 1950 2463
Size: 872 11 40 521 118 336 51 513
Cuts at: 2463 2583 2865 2997 3114 3581 3604 3939 4185
Size: 120 282 132 117 467 23 335 246
Cuts at: 4185 4491 4503 4589 5641 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 872 521 513 467 449 336 335
306 282 246 132 120 118 117 86
51 40 23 12 11
Bsu36I CC'TnA_GG
Cuts at: 383 383
Size: 6089
BtgI C'CryG_G
Cuts at: 2788 2788
Size: 6089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2410 4039 4220 4383 2410
Size: 1629 181 163 4116
Fragments arranged by size:
4116 1629 181 163
BtsI GCAGTG_nn'
Cuts at: 632 2370 3332 632
Size: 1738 962 3389
Fragments arranged by size:
3389 1738 962
DraI TTT'AAA
Cuts at: 3088 3088
Size: 6089
DraIII CAC_nnn'GTG
Cuts at: 1842 2317 1842
Size: 475 5614
DrdI GACnn_nn'nnGTC
Cuts at: 3795 5139 3795
Size: 1344 4745
EaeI y'GGCC_r
Cuts at: 362 1543 3674 3848 4239 4266 5611 362
Size: 1181 2131 174 391 27 1345 840
Fragments arranged by size:
2131 1345 1181 840 391 174 27
EagI C'GGCC_G
Cuts at: 1543 3674 1543
Size: 2131 3958
EarI CTCTTCn'nnn_
Cuts at: 4112 4322 4112
Size: 210 5879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4297 5039 5185 5899 4297
Size: 742 146 714 4487
Fragments arranged by size:
4487 742 714 146
EcoICRI GAG'CTC
Cuts at: 1583 3101 1583
Size: 1518 4571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 291 311 338 749 1010 1070 1246 1259
Size: 163 20 27 411 261 60 176 13
Cuts at: 1259 1385 1534 1638 1906 2291 2338 2345 2731
Size: 126 149 104 268 385 47 7 386
Cuts at: 2731 2944 3914 4346 4699 5361 128
Size: 213 970 432 353 662 856
Fragments arranged by size:
970 856 662 432 411 386 385 353
268 261 213 176 163 149 126 104
60 47 27 20 13 7
EcoNI CCTnn'n_nnAGG
Cuts at: 673 2480 673
Size: 1807 4282
EcoO109I rG'GnC_Cy
Cuts at: 1078 1195 1930 2666 2801 3228 1078
Size: 117 735 736 135 427 3939
Fragments arranged by size:
3939 736 735 427 135 117
EcoRI G'AATT_C
Cuts at: 1431 2989 3093 1431
Size: 1558 104 4427
Fragments arranged by size:
4427 1558 104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 807 4108 807
Size: 3301 2788
FspI TGC'GCA
Cuts at: 3870 3870
Size: 6089
HaeII r_GCGC'y
Cuts at: 2724 3523 3771 5001 5772 2724
Size: 799 248 1230 771 3041
Fragments arranged by size:
3041 1230 799 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 543 575 1213 1245 1567 1599 2368 2400 2898
Size: 32 638 32 322 32 769 32 498
Cuts at: 2898 2930 3931 3961 3963 3993 543
Size: 32 1001 30 2 30 2639
Fragments arranged by size:
2639 1001 769 638 498 322 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 246 334 3128 246
Size: 88 2794 3207
Fragments arranged by size:
3207 2794 88
KasI G'GCGC_C
Cuts at: 3519 3767 3519
Size: 248 5841
KpnI G_GTAC'C
Cuts at: 462 3109 462
Size: 2647 3442
MluI A'CGCG_T
Cuts at: 742 5522 742
Size: 4780 1309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4848 5032 4848
Size: 184 5905
MscI TGG'CCA
Cuts at: 364 3850 364
Size: 3486 2603
MslI CAynn'nnrTG
Cuts at: 427 1267 4078 4205 427
Size: 840 2811 127 2311
Fragments arranged by size:
2811 2311 840 127
MspA1I CmG'CkG
Cuts at: 148 399 1236 1814 1827 3200 3514 3874 4658
Size: 251 837 578 13 1373 314 360 784
Cuts at: 4658 4903 148
Size: 245 1334
Fragments arranged by size:
1373 1334 837 784 578 360 314 251
245 13
NaeI GCC'GGC
Cuts at: 2902 4270 2902
Size: 1368 4721
NarI GG'CG_CC
Cuts at: 3520 3768 3520
Size: 248 5841
NgoMIV G'CCGG_C
Cuts at: 2900 4268 2900
Size: 1368 4721
NheI G'CTAG_C
Cuts at: 2266 2266
Size: 6089
NspI r_CATG'y
Cuts at: 839 2640 3142 3642 5245 839
Size: 1801 502 500 1603 1683
Fragments arranged by size:
1801 1683 1603 502 500
PciI A'CATG_T
Cuts at: 5241 5241
Size: 6089
PflMI CCAn_nnn'nTGG
Cuts at: 819 1338 819
Size: 519 5570
PfoI T'CCnGG_A
Cuts at: 134 524 2354 2547 2921 2984 134
Size: 390 1830 193 374 63 3239
Fragments arranged by size:
3239 1830 390 374 193 63
PmeI GTTT'AAAC
Cuts at: 3088 3088
Size: 6089
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 4535
Size: 32 6057
PpuMI rG'GwC_Cy
Cuts at: 1195 1930 2666 2801 1195
Size: 735 736 135 4483
Fragments arranged by size:
4483 736 735 135
PstI C_TGCA'G
Cuts at: 608 639 1077 1823 1877 3136 3821 608
Size: 31 438 746 54 1259 685 2876
Fragments arranged by size:
2876 1259 746 685 438 54 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6089
PvuII CAG'CTG
Cuts at: 148 399 1827 3514 3874 148
Size: 251 1428 1687 360 2363
Fragments arranged by size:
2363 1687 1428 360 251
RsrII CG'GwC_CG
Cuts at: 4284 4284
Size: 6089
SacI G_AGCT'C
Cuts at: 1585 3103 1585
Size: 1518 4571
SalI G'TCGA_C
Cuts at: 332 3126 332
Size: 2794 3295
SanDI GG'GwC_CC
Cuts at: 2666 2666
Size: 6089
SapI GCTCTTCn'nnn_
Cuts at: 4112 4322 4112
Size: 210 5879
SbfI CC_TGCA'GG
Cuts at: 3136 3136
Size: 6089
ScaI AGT'ACT
Cuts at: 5606 5606
Size: 6089
SexAI A'CCwGG_T
Cuts at: 1629 1629
Size: 6089
SfcI C'TryA_G
Cuts at: 33 604 635 1073 1112 1177 1819 1873 3132
Size: 571 31 438 39 65 642 54 1259
Cuts at: 3132 3817 4785 4976 33
Size: 685 968 191 1146
Fragments arranged by size:
1259 1146 968 685 642 571 438 191
65 54 39 31
SfoI GGC'GCC
Cuts at: 3521 3769 3521
Size: 248 5841
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6089
SmaI CCC'GGG
Cuts at: 3111 3111
Size: 6089
SmlI C'TyrA_G
Cuts at: 3244 4159 4873 5135 3244
Size: 915 714 262 4198
Fragments arranged by size:
4198 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6089
SphI G_CATG'C
Cuts at: 3142 3642 3142
Size: 500 5589
StuI AGG'CCT
Cuts at: 1412 1412
Size: 6089
StyI C'CwwG_G
Cuts at: 914 2970 3223 3577 914
Size: 2056 253 354 3426
Fragments arranged by size:
3426 2056 354 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1117 1772 2248 2715 3061 3672 3805 4264 4473
Size: 655 476 467 346 611 133 459 209
Cuts at: 4473 1117
Size: 2733
Fragments arranged by size:
2733 655 611 476 467 459 346 209
133
TatI w'GTAC_w
Cuts at: 894 5604 894
Size: 4710 1379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 357 389 1297 1329 5606 5638 357
Size: 32 908 32 4277 32 808
Fragments arranged by size:
4277 908 808 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3886 3886
Size: 6089
XbaI T'CTAG_A
Cuts at: 2128 3120 2128
Size: 992 5097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 516 789 2394 2487 3584 516
Size: 273 1605 93 1097 3021
Fragments arranged by size:
3021 1605 1097 273 93
XmaI C'CCGG_G
Cuts at: 3109 3109
Size: 6089
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI ApaI AscI AvrII
BcgI BfrBI BglI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI