Restriction map for
pF1KA0247
(Circular) (Six-base) MAPSORT of: pF1KA0247.seq Check: 4599 from: 1 to: 4022
pF1KA0247 4022 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 687 775 687
Size: 88 3934
AccI GT'mk_AC
Cuts at: 1060 1060
Size: 4022
Acc65I G'GTAC_C
Cuts at: 1038 1038
Size: 4022
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 565 1847 2279 2632 3294 353
Size: 212 1282 432 353 662 1081
Fragments arranged by size:
1282 1081 662 432 353 212
AflIII A'CryG_T
Cuts at: 341 370 1374 3174 3455 341
Size: 29 1004 1800 281 908
Fragments arranged by size:
1800 1004 908 281 29
AleI CACnn'nnGTG
Cuts at: 142 142
Size: 4022
AlwNI CAG_nnn'CTG
Cuts at: 853 1327 2765 853
Size: 474 1438 2110
Fragments arranged by size:
2110 1438 474
ApaI G_GGCC'C
Cuts at: 725 725
Size: 4022
ApaLI G'TGCA_C
Cuts at: 2860 2860
Size: 4022
ApoI r'AATT_y
Cuts at: 1026 3375 3631 1026
Size: 2349 256 1417
Fragments arranged by size:
2349 1417 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 839 1042 839
Size: 203 3819
BamHI G'GATC_C
Cuts at: 1047 1047
Size: 4022
BanI G'GyrC_C
Cuts at: 1038 1452 1700 1735 1038
Size: 414 248 35 3325
Fragments arranged by size:
3325 414 248 35
BanII G_rGCy'C
Cuts at: 725 838 1036 2066 725
Size: 113 198 1030 2681
Fragments arranged by size:
2681 1030 198 113
BbeI G_GCGC'C
Cuts at: 1456 1704 1456
Size: 248 3774
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 297 331 297
Size: 34 3988
BciVI GTATCCnnnnn_n'
Cuts at: 1920 2976 4022 1920
Size: 1056 1046 1920
Fragments arranged by size:
1920 1056 1046
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BglII A'GATC_T
Cuts at: 1537 3574 1537
Size: 2037 1985
BlpI GC'TnA_GC
Cuts at: 1134 1134
Size: 4022
Bme1580I G_kGCm'C
Cuts at: 235 725 1647 1740 2864 235
Size: 490 922 93 1124 1393
Fragments arranged by size:
1393 1124 922 490 93
BmgBI CAC'GTC
Cuts at: 371 974 371
Size: 603 3419
BmrI ACTGGGnnnn_n'
Cuts at: 719 1234 1315 1650 3794 719
Size: 515 81 335 2144 947
Fragments arranged by size:
2144 947 515 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4022
Bpu10I CC'TnA_GC
Cuts at: 897 897
Size: 4022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 2077 2791 3089 1198
Size: 879 714 298 2131
Fragments arranged by size:
2131 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 284 600 3726 284
Size: 316 3126 580
Fragments arranged by size:
3126 580 316
BsaAI yAC'GTr
Cuts at: 1375 1375
Size: 4022
BsaBI GATnn'nnATC
Cuts at: 6 1536 3573 6
Size: 1530 2037 455
Fragments arranged by size:
2037 1530 455
BsaHI Gr'CG_yC
Cuts at: 1453 1701 2403 3899 1453
Size: 248 702 1496 1576
Fragments arranged by size:
1576 1496 702 248
BsaWI w'CCGG_w
Cuts at: 243 1211 1732 2410 2821 2968 3242 243
Size: 968 521 678 411 147 274 1023
Fragments arranged by size:
1023 968 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 580 605 610 635 580
Size: 25 5 25 3967
Fragments arranged by size:
3967 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 499 655 747 768 994 496
Size: 3 156 92 21 226 3524
Fragments arranged by size:
3524 226 156 92 21 3
BseYI C'CCAG_C
Cuts at: 1447 2205 2870 3278 1447
Size: 758 665 408 2191
Fragments arranged by size:
2191 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1564 1610 2840 104
Size: 1460 46 1230 1286
Fragments arranged by size:
1460 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 139 1036 1814 2004 2864 139
Size: 897 778 190 860 1297
Fragments arranged by size:
1297 897 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 206 421 206
Size: 215 3807
Bsp1286I G_dGCh'C
Cuts at: 139 235 725 838 1036 1647 1740 1814 2004
Size: 96 490 113 198 611 93 74 190
Cuts at: 2004 2066 2864 139
Size: 62 798 1297
Fragments arranged by size:
1297 798 611 490 198 190 113 96
93 74 62
BspHI T'CATG_A
Cuts at: 2454 2454
Size: 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 687 775 1072 1286 1588 1969 687
Size: 88 297 214 302 381 2740
Fragments arranged by size:
2740 381 302 297 214 88
BsrBI CCG'CTC
Cuts at: 50 2314 2368 3852 4017 50
Size: 2264 54 1484 165 55
Fragments arranged by size:
2264 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1934 1934
Size: 4022
BsrFI r'CCGG_y
Cuts at: 892 2020 2201 892
Size: 1128 181 2713
Fragments arranged by size:
2713 1128 181
BssSI C'ACGA_G
Cuts at: 511 2293 3001 511
Size: 1782 708 1532
Fragments arranged by size:
1782 1532 708
BstAPI GCAn_nnn'nTGC
Cuts at: 756 1399 756
Size: 643 3379
BstBI TT'CG_AA
Cuts at: 2383 2383
Size: 4022
BstXI CCAn_nnnn'nTGG
Cuts at: 492 606 492
Size: 114 3908
BstYI r'GATC_y
Cuts at: 1 1047 1514 1537 1872 2118 2424 2436 2522
Size: 1046 467 23 335 246 306 12 86
Cuts at: 2522 3574 1
Size: 1052 449
Fragments arranged by size:
1052 1046 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 114 152 161 398 491 114
Size: 38 9 237 93 3645
Fragments arranged by size:
3645 237 93 38 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1972 2153 2316 1972
Size: 181 163 3678
Fragments arranged by size:
3678 181 163
BtsI GCAGTG_nn'
Cuts at: 315 1265 315
Size: 950 3072
DraI TTT'AAA
Cuts at: 1021 1021
Size: 4022
DraIII CAC_nnn'GTG
Cuts at: 672 672
Size: 4022
DrdI GACnn_nn'nnGTC
Cuts at: 1728 3072 1728
Size: 1344 2678
EaeI y'GGCC_r
Cuts at: 890 1607 1781 2172 2199 3544 890
Size: 717 174 391 27 1345 1368
Fragments arranged by size:
1368 1345 717 391 174 27
EagI C'GGCC_G
Cuts at: 1607 1607
Size: 4022
EarI CTCTTCn'nnn_
Cuts at: 885 2045 2255 885
Size: 1160 210 2652
Fragments arranged by size:
2652 1160 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2230 2972 3118 3832 2230
Size: 742 146 714 2420
Fragments arranged by size:
2420 742 714 146
EcoICRI GAG'CTC
Cuts at: 1034 1034
Size: 4022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 565 1847 2279 2632 3294 353
Size: 212 1282 432 353 662 1081
Fragments arranged by size:
1282 1081 662 432 353 212
EcoO109I rG'GnC_Cy
Cuts at: 453 770 801 1161 453
Size: 317 31 360 3314
Fragments arranged by size:
3314 360 317 31
EcoRI G'AATT_C
Cuts at: 1026 1026
Size: 4022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 4022
FspI TGC'GCA
Cuts at: 1803 1803
Size: 4022
HaeII r_GCGC'y
Cuts at: 499 1456 1704 2934 3705 499
Size: 957 248 1230 771 816
Fragments arranged by size:
1230 957 816 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 849 881 1864 1894 1896 1926 580
Size: 32 237 32 983 30 2 30 2676
Fragments arranged by size:
2676 983 237 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1061 1061
Size: 4022
KasI G'GCGC_C
Cuts at: 1452 1700 1452
Size: 248 3774
KpnI G_GTAC'C
Cuts at: 1042 1042
Size: 4022
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2781 2965 2781
Size: 184 3838
MscI TGG'CCA
Cuts at: 1783 1783
Size: 4022
MslI CAynn'nnrTG
Cuts at: 142 604 2011 2138 142
Size: 462 1407 127 2026
Fragments arranged by size:
2026 1407 462 127
MspA1I CmG'CkG
Cuts at: 756 1133 1447 1807 2591 2836 756
Size: 377 314 360 784 245 1942
Fragments arranged by size:
1942 784 377 360 314 245
NaeI GCC'GGC
Cuts at: 894 2203 894
Size: 1309 2713
NarI GG'CG_CC
Cuts at: 1453 1701 1453
Size: 248 3774
NcoI C'CATG_G
Cuts at: 114 398 114
Size: 284 3738
NgoMIV G'CCGG_C
Cuts at: 892 2201 892
Size: 1309 2713
NspI r_CATG'y
Cuts at: 345 1075 1575 3178 345
Size: 730 500 1603 1189
Fragments arranged by size:
1603 1189 730 500
PciI A'CATG_T
Cuts at: 341 3174 341
Size: 2833 1189
PflMI CCAn_nnn'nTGG
Cuts at: 256 256
Size: 4022
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2500 2468
Size: 32 3990
PpuMI rG'GwC_Cy
Cuts at: 453 453
Size: 4022
PspOMI G'GGCC_C
Cuts at: 721 721
Size: 4022
PstI C_TGCA'G
Cuts at: 289 536 786 933 1069 1754 289
Size: 247 250 147 136 685 2557
Fragments arranged by size:
2557 685 250 247 147 136
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 756 1447 1807 756
Size: 691 360 2971
Fragments arranged by size:
2971 691 360
RsrII CG'GwC_CG
Cuts at: 2217 2217
Size: 4022
SacI G_AGCT'C
Cuts at: 1036 1036
Size: 4022
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SanDI GG'GwC_CC
Cuts at: 453 453
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
SbfI CC_TGCA'GG
Cuts at: 1069 1069
Size: 4022
ScaI AGT'ACT
Cuts at: 3539 3539
Size: 4022
SexAI A'CCwGG_T
Cuts at: 590 590
Size: 4022
SfcI C'TryA_G
Cuts at: 33 285 467 532 782 929 1065 1750 2718
Size: 252 182 65 250 147 136 685 968
Cuts at: 2718 2909 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 252 250 191 182 147
136 65
SfoI GGC'GCC
Cuts at: 1454 1702 1454
Size: 248 3774
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SmaI CCC'GGG
Cuts at: 1044 1044
Size: 4022
SmlI C'TyrA_G
Cuts at: 1177 2092 2806 3068 1177
Size: 915 714 262 2131
Fragments arranged by size:
2131 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 1075 1575 1075
Size: 500 3522
StyI C'CwwG_G
Cuts at: 114 398 742 765 1156 1510 114
Size: 284 344 23 391 354 2626
Fragments arranged by size:
2626 391 354 344 284 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 451 876 1605 1738 2197 2406 451
Size: 425 729 133 459 209 2067
Fragments arranged by size:
2067 729 459 425 209 133
TatI w'GTAC_w
Cuts at: 693 983 3537 693
Size: 290 2554 1178
Fragments arranged by size:
2554 1178 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 158 190 437 469 3539 3571 158
Size: 32 247 32 3070 32 609
Fragments arranged by size:
3070 609 247 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 592 1819 592
Size: 1227 2795
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 445 580 1517 445
Size: 135 937 2950
Fragments arranged by size:
2950 937 135
XmaI C'CCGG_G
Cuts at: 1042 1042
Size: 4022
XmnI GAAnn'nnTTC
Cuts at: 550 550
Size: 4022
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmtI BpmI
BsgI BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI