Restriction map for
pF1KA0240
(Circular) (Six-base) MAPSORT of: pF1KA0240.seq Check: 4986 from: 1 to: 6350
pF1KA0240 6350 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2142 2142
Size: 6350
AccI GT'mk_AC
Cuts at: 1888 2552 3388 1888
Size: 664 836 4850
Fragments arranged by size:
4850 836 664
Acc65I G'GTAC_C
Cuts at: 3366 3366
Size: 6350
AclI AA'CG_TT
Cuts at: 6190 6190
Size: 6350
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 1884 2363 2882 2911 4175 4607 5354 5622
Size: 1475 479 519 29 1264 432 747 268
Cuts at: 5622 409
Size: 1137
Fragments arranged by size:
1475 1264 1137 747 519 479 432 268
29
AflIII A'CryG_T
Cuts at: 678 1181 2318 3007 3702 4806 5783 678
Size: 503 1137 689 695 1104 977 1245
Fragments arranged by size:
1245 1137 1104 977 695 689 503
AgeI A'CCGG_T
Cuts at: 2707 4738 2707
Size: 2031 4319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1556 2062 2680 1556
Size: 506 618 5226
Fragments arranged by size:
5226 618 506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 900 901 932 933 900
Size: 1 31 1 6317
Fragments arranged by size:
6317 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 594 2304 3655 5222 594
Size: 1710 1351 1567 1722
Fragments arranged by size:
1722 1710 1567 1351
ApaI G_GGCC'C
Cuts at: 323 323
Size: 6350
ApaLI G'TGCA_C
Cuts at: 5120 5120
Size: 6350
ApoI r'AATT_y
Cuts at: 243 963 1020 1103 2371 2821 3042 3354 5703
Size: 720 57 83 1268 450 221 312 2349
Cuts at: 5703 5959 243
Size: 256 634
Fragments arranged by size:
2349 1268 720 634 450 312 256 221
83 57
AseI AT'TA_AT
Cuts at: 20 791 1040 20
Size: 771 249 5330
Fragments arranged by size:
5330 771 249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6350
AvaI C'yCGr_G
Cuts at: 373 2528 3370 373
Size: 2155 842 3353
Fragments arranged by size:
3353 2155 842
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1826 1859 1826
Size: 33 6317
BamHI G'GATC_C
Cuts at: 3375 3375
Size: 6350
BanI G'GyrC_C
Cuts at: 2924 3366 3780 4028 4063 2924
Size: 442 414 248 35 5211
Fragments arranged by size:
5211 442 414 248 35
BanII G_rGCy'C
Cuts at: 323 866 1165 2033 2332 3364 4394 323
Size: 543 299 868 299 1032 1030 2279
Fragments arranged by size:
2279 1032 1030 868 543 299 299
BbeI G_GCGC'C
Cuts at: 3784 4032 3784
Size: 248 6102
BbsI GAAGACnn'nnnn_
Cuts at: 371 1677 1858 2354 371
Size: 1306 181 496 4367
Fragments arranged by size:
4367 1306 496 181
BbvCI CC'TCA_GC
Cuts at: 1583 1583
Size: 6350
BciVI GTATCCnnnnn_n'
Cuts at: 3343 4248 5009 6350 3343
Size: 905 761 1341 3343
Fragments arranged by size:
3343 1341 905 761
BclI T'GATC_A
Cuts at: 3870 3870
Size: 6350
BglII A'GATC_T
Cuts at: 3105 3865 5902 3105
Size: 760 2037 3553
Fragments arranged by size:
3553 2037 760
BlpI GC'TnA_GC
Cuts at: 524 1740 3462 524
Size: 1216 1722 3412
Fragments arranged by size:
3412 1722 1216
Bme1580I G_kGCm'C
Cuts at: 323 2927 3975 4068 5124 323
Size: 2604 1048 93 1056 1549
Fragments arranged by size:
2604 1549 1056 1048 93
BmgBI CAC'GTC
Cuts at: 1356 2858 3008 1356
Size: 1502 150 4698
Fragments arranged by size:
4698 1502 150
BmrI ACTGGGnnnn_n'
Cuts at: 317 1259 3562 3643 3978 6122 317
Size: 942 2303 81 335 2144 545
Fragments arranged by size:
2303 2144 942 545 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 3062 4717 2903
Size: 159 1655 4536
Fragments arranged by size:
4536 1655 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1823 2206 3094 337
Size: 1486 383 888 3593
Fragments arranged by size:
3593 1486 888 383
Bpu10I CC'TnA_GC
Cuts at: 1462 1583 1462
Size: 121 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2055 2373 3526 4405 4897 5195 2055
Size: 318 1153 879 492 298 3210
Fragments arranged by size:
3210 1153 879 492 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 143 2687 2772 6054 143
Size: 2544 85 3282 439
Fragments arranged by size:
3282 2544 439 85
BsaAI yAC'GTr
Cuts at: 1119 2319 3703 1119
Size: 1200 1384 3766
Fragments arranged by size:
3766 1384 1200
BsaBI GATnn'nnATC
Cuts at: 6 3864 5901 6
Size: 3858 2037 455
Fragments arranged by size:
3858 2037 455
BsaHI Gr'CG_yC
Cuts at: 3781 4029 4731 6227 3781
Size: 248 702 1496 3904
Fragments arranged by size:
3904 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2707 2927 3539 4060 4738 5012 5159 5570 2707
Size: 220 612 521 678 274 147 411 3487
Fragments arranged by size:
3487 678 612 521 411 274 220 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2992 3022 2992
Size: 30 6320
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 374 1571 374
Size: 1197 5153
BseYI C'CCAG_C
Cuts at: 697 1823 1925 3098 3775 4533 5110 5606 697
Size: 1126 102 1173 677 758 577 496 1441
Fragments arranged by size:
1441 1173 1126 758 677 577 496 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 2863 2170
Size: 693 5657
BsiEI CG_ry'CG
Cuts at: 104 3892 3938 5146 104
Size: 3788 46 1208 1308
Fragments arranged by size:
3788 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2041 2817 2857 2908 3364 4142 4332 5124 2041
Size: 776 40 51 456 778 190 792 3267
Fragments arranged by size:
3267 792 778 776 456 190 51 40
BsmI GAATG_Cn'
Cuts at: 2806 2806
Size: 6350
Bsp1286I G_dGCh'C
Cuts at: 323 866 1165 2033 2041 2332 2817 2857 2908
Size: 543 299 868 8 291 485 40 51
Cuts at: 2908 2927 3364 3975 4068 4142 4332 4394 5124
Size: 19 437 611 93 74 190 62 730
Cuts at: 5124 323
Size: 1549
Fragments arranged by size:
1549 868 730 611 543 485 437 299
291 190 93 74 62 51 40 19
8
BspEI T'CCGG_A
Cuts at: 5570 5570
Size: 6350
BspHI T'CATG_A
Cuts at: 2610 2730 3077 5526 2610
Size: 120 347 2449 3434
Fragments arranged by size:
3434 2449 347 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2142 3400 3614 3916 4297 2142
Size: 1258 214 302 381 4195
Fragments arranged by size:
4195 1258 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4642 4696 6180 6345 50
Size: 4592 54 1484 165 55
Fragments arranged by size:
4592 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 207 4262 207
Size: 4055 2295
BsrFI r'CCGG_y
Cuts at: 2707 4348 4529 4738 2707
Size: 1641 181 209 4319
Fragments arranged by size:
4319 1641 209 181
BsrGI T'GTAC_A
Cuts at: 657 657
Size: 6350
BssSI C'ACGA_G
Cuts at: 126 4621 4979 126
Size: 4495 358 1497
Fragments arranged by size:
4495 1497 358
BstAPI GCAn_nnn'nTGC
Cuts at: 637 3727 637
Size: 3090 3260
BstBI TT'CG_AA
Cuts at: 4711 4711
Size: 6350
BstYI r'GATC_y
Cuts at: 1 805 1130 2021 3105 3375 3842 3865 4200
Size: 804 325 891 1084 270 467 23 335
Cuts at: 4200 4446 5458 5544 5556 5902 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1084 1012 891 804 467 449 346 335
325 270 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2008 2088 2558 2918 2008
Size: 80 470 360 5440
Fragments arranged by size:
5440 470 360 80
BtgI C'CryG_G
Cuts at: 107 1277 3191 107
Size: 1170 1914 3266
Fragments arranged by size:
3266 1914 1170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4300 4481 4644 4300
Size: 181 163 6006
Fragments arranged by size:
6006 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1748 2044 3593 1244
Size: 504 296 1549 4001
Fragments arranged by size:
4001 1549 504 296
ClaI AT'CG_AT
Cuts at: 2489 2489
Size: 6350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 143 178 143
Size: 35 6315
DraI TTT'AAA
Cuts at: 3349 3349
Size: 6350
DraIII CAC_nnn'GTG
Cuts at: 1524 2253 2287 2763 1524
Size: 729 34 476 5111
Fragments arranged by size:
5111 729 476 34
DrdI GACnn_nn'nnGTC
Cuts at: 1707 4056 4914 1707
Size: 2349 858 3143
Fragments arranged by size:
3143 2349 858
EaeI y'GGCC_r
Cuts at: 1410 2136 2625 3935 4109 4500 4527 5872 1410
Size: 726 489 1310 174 391 27 1345 1888
Fragments arranged by size:
1888 1345 1310 726 489 391 174 27
EagI C'GGCC_G
Cuts at: 3935 3935
Size: 6350
EarI CTCTTCn'nnn_
Cuts at: 392 467 1455 2858 4373 4583 392
Size: 75 988 1403 1515 210 2159
Fragments arranged by size:
2159 1515 1403 988 210 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4558 4868 5014 6160 4558
Size: 310 146 1146 4748
Fragments arranged by size:
4748 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3362 3362
Size: 6350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 409 1823 1884 2206 2363 2882 2911 3094
Size: 72 1414 61 322 157 519 29 183
Cuts at: 3094 4175 4607 5354 5622 337
Size: 1081 432 747 268 1065
Fragments arranged by size:
1414 1081 1065 747 519 432 322 268
183 157 72 61 29
EcoNI CCTnn'n_nnAGG
Cuts at: 1916 1916
Size: 6350
EcoO109I rG'GnC_Cy
Cuts at: 319 1714 2405 3489 319
Size: 1395 691 1084 3180
Fragments arranged by size:
3180 1395 1084 691
EcoRI G'AATT_C
Cuts at: 1103 3042 3354 1103
Size: 1939 312 4099
Fragments arranged by size:
4099 1939 312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 544 2434 3165 4369 544
Size: 1890 731 1204 2525
Fragments arranged by size:
2525 1890 1204 731
FspI TGC'GCA
Cuts at: 4131 4131
Size: 6350
HaeII r_GCGC'y
Cuts at: 3784 4032 5054 6033 3784
Size: 248 1022 979 4101
Fragments arranged by size:
4101 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 265 297 797 829 1554 1586 2423
Size: 32 124 32 500 32 725 32 837
Cuts at: 2423 2455 4192 4222 4224 4254 109
Size: 32 1737 30 2 30 2205
Fragments arranged by size:
2205 1737 837 725 500 124 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3389 3389
Size: 6350
KasI G'GCGC_C
Cuts at: 3780 4028 3780
Size: 248 6102
KpnI G_GTAC'C
Cuts at: 3370 3370
Size: 6350
MluI A'CGCG_T
Cuts at: 5783 5783
Size: 6350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 606 1364 1478 2103 5021 5205 606
Size: 758 114 625 2918 184 1751
Fragments arranged by size:
2918 1751 758 625 184 114
MscI TGG'CCA
Cuts at: 1412 2138 4111 1412
Size: 726 1973 3651
Fragments arranged by size:
3651 1973 726
MslI CAynn'nnrTG
Cuts at: 1186 4339 4466 1186
Size: 3153 127 3070
Fragments arranged by size:
3153 3070 127
MspA1I CmG'CkG
Cuts at: 830 1818 2146 3114 3322 3461 3775 4135 5148
Size: 988 328 968 208 139 314 360 1013
Cuts at: 5148 5393 830
Size: 245 1787
Fragments arranged by size:
1787 1013 988 968 360 328 314 245
208 139
NaeI GCC'GGC
Cuts at: 4531 4531
Size: 6350
NarI GG'CG_CC
Cuts at: 3781 4029 3781
Size: 248 6102
NcoI C'CATG_G
Cuts at: 107 1277 107
Size: 1170 5180
NgoMIV G'CCGG_C
Cuts at: 4529 4529
Size: 6350
NspI r_CATG'y
Cuts at: 619 682 1185 1432 1461 1710 3403 3903 4810
Size: 63 503 247 29 249 1693 500 907
Cuts at: 4810 619
Size: 2159
Fragments arranged by size:
2159 1693 907 503 500 249 247 63
29
PasI CC'CwG_GG
Cuts at: 1158 2026 1158
Size: 868 5482
PciI A'CATG_T
Cuts at: 678 1181 4806 678
Size: 503 3625 2222
Fragments arranged by size:
3625 2222 503
PflMI CCAn_nnn'nTGG
Cuts at: 1483 1483
Size: 6350
PfoI T'CCnGG_A
Cuts at: 3023 3272 3023
Size: 249 6101
PmeI GTTT'AAAC
Cuts at: 3349 3349
Size: 6350
PmlI CAC'GTG
Cuts at: 2319 2319
Size: 6350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1432 1464 5489 5521 1432
Size: 32 4025 32 2261
Fragments arranged by size:
4025 2261 32 32
PpuMI rG'GwC_Cy
Cuts at: 1714 2405 1714
Size: 691 5659
PshAI GACnn'nnGTC
Cuts at: 128 2267 3032 128
Size: 2139 765 3446
Fragments arranged by size:
3446 2139 765
PspOMI G'GGCC_C
Cuts at: 319 319
Size: 6350
PspXI vC'TCGA_Gb
Cuts at: 373 373
Size: 6350
PstI C_TGCA'G
Cuts at: 234 599 1776 2775 3397 4082 234
Size: 365 1177 999 622 685 2502
Fragments arranged by size:
2502 1177 999 685 622 365
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6350
PvuII CAG'CTG
Cuts at: 1818 2146 3114 3322 3775 4135 1818
Size: 328 968 208 453 360 4033
Fragments arranged by size:
4033 968 453 360 328 208
RsrII CG'GwC_CG
Cuts at: 4545 4545
Size: 6350
SacI G_AGCT'C
Cuts at: 3364 3364
Size: 6350
SalI G'TCGA_C
Cuts at: 3387 3387
Size: 6350
SapI GCTCTTCn'nnn_
Cuts at: 4373 4583 4373
Size: 210 6140
SbfI CC_TGCA'GG
Cuts at: 3397 3397
Size: 6350
ScaI AGT'ACT
Cuts at: 5867 5867
Size: 6350
SfcI C'TryA_G
Cuts at: 33 230 595 602 1772 2771 3393 4078 5071
Size: 197 365 7 1170 999 622 685 993
Cuts at: 5071 5262 33
Size: 191 1121
Fragments arranged by size:
1170 1121 999 993 685 622 365 197
191 7
SfoI GGC'GCC
Cuts at: 3782 4030 3782
Size: 248 6102
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6350
SmaI CCC'GGG
Cuts at: 3372 3372
Size: 6350
SmlI C'TyrA_G
Cuts at: 373 2034 2352 3505 4420 4912 5174 373
Size: 1661 318 1153 915 492 262 1549
Fragments arranged by size:
1661 1549 1153 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6350
SphI G_CATG'C
Cuts at: 3403 3903 3403
Size: 500 5850
StuI AGG'CCT
Cuts at: 2184 2184
Size: 6350
StyI C'CwwG_G
Cuts at: 107 1277 1665 2678 2685 2805 3484 3838 107
Size: 1170 388 1013 7 120 679 354 2619
Fragments arranged by size:
2619 1170 1013 679 388 354 120 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 643 1503 1582 2711 3933 4066 4525 4734 643
Size: 860 79 1129 1222 133 459 209 2259
Fragments arranged by size:
2259 1222 1129 860 459 209 133 79
TatI w'GTAC_w
Cuts at: 657 5865 657
Size: 5208 1142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1544 1576 1740 1772 2992 3024 5867 5899 1544
Size: 32 164 32 1220 32 2843 32 1995
Fragments arranged by size:
2843 1995 1220 164 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1718 4147 1718
Size: 2429 3921
XbaI T'CTAG_A
Cuts at: 3381 3381
Size: 6350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1366 1480 1525 1672 1762 3845 1366
Size: 114 45 147 90 2083 3871
Fragments arranged by size:
3871 2083 147 114 90 45
XhoI C'TCGA_G
Cuts at: 373 373
Size: 6350
XmaI C'CCGG_G
Cuts at: 3370 3370
Size: 6350
XmnI GAAnn'nnTTC
Cuts at: 1449 2784 1449
Size: 1335 5015
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BcgI
BfrBI BglI BmtI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PsiI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI