Restriction map for
pF1KA0236
(Circular) (Six-base) MAPSORT of: pF1KA0236.seq Check: 5584 from: 1 to: 8174
pF1KA0236 8174 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 659 2478 2768 2898 5108 659
Size: 1819 290 130 2210 3725
Fragments arranged by size:
3725 2210 1819 290 130
AccI GT'mk_AC
Cuts at: 5212 5212
Size: 8174
Acc65I G'GTAC_C
Cuts at: 2848 3743 3820 5190 2848
Size: 895 77 1370 5832
Fragments arranged by size:
5832 1370 895 77
AclI AA'CG_TT
Cuts at: 8014 8014
Size: 8174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 482 1048 1839 2384 2785 3175 3266 4310
Size: 156 566 791 545 401 390 91 1044
Cuts at: 4310 4726 5999 6431 6784 7446 326
Size: 416 1273 432 353 662 1054
Fragments arranged by size:
1273 1054 1044 791 662 566 545 432
416 401 390 353 156 91
AfeI AGC'GCT
Cuts at: 616 616
Size: 8174
AflII C'TTAA_G
Cuts at: 3555 4009 3555
Size: 454 7720
AflIII A'CryG_T
Cuts at: 1478 3430 5526 7326 7607 1478
Size: 1952 2096 1800 281 2045
Fragments arranged by size:
2096 2045 1952 1800 281
AgeI A'CCGG_T
Cuts at: 3746 3845 3746
Size: 99 8075
AleI CACnn'nnGTG
Cuts at: 785 1397 785
Size: 612 7562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2776 3602 2776
Size: 826 7348
AlwNI CAG_nnn'CTG
Cuts at: 304 349 549 2073 2395 2986 3524 5479 6917
Size: 45 200 1524 322 591 538 1955 1438
Cuts at: 6917 304
Size: 1561
Fragments arranged by size:
1955 1561 1524 1438 591 538 322 200
45
ApaI G_GGCC'C
Cuts at: 1969 3608 4087 1969
Size: 1639 479 6056
Fragments arranged by size:
6056 1639 479
ApaLI G'TGCA_C
Cuts at: 1482 1528 4467 4765 5095 7012 1482
Size: 46 2939 298 330 1917 2644
Fragments arranged by size:
2939 2644 1917 330 298 46
ApoI r'AATT_y
Cuts at: 889 5178 7527 7783 889
Size: 4289 2349 256 1280
Fragments arranged by size:
4289 2349 1280 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8174
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8174
AvaI C'yCGr_G
Cuts at: 245 2713 3493 4036 4274 4723 5194 245
Size: 2468 780 543 238 449 471 3225
Fragments arranged by size:
3225 2468 780 543 471 449 238
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4612 4645 4612
Size: 33 8141
BamHI G'GATC_C
Cuts at: 4607 5121 5199 4607
Size: 514 78 7582
Fragments arranged by size:
7582 514 78
BanI G'GyrC_C
Cuts at: 571 2681 2848 3578 3743 3820 3952 5033 5190
Size: 2110 167 730 165 77 132 1081 157
Cuts at: 5190 5604 5852 5887 571
Size: 414 248 35 2858
Fragments arranged by size:
2858 2110 1081 730 414 248 167 165
157 132 77 35
BanII G_rGCy'C
Cuts at: 235 543 1969 1994 2402 2702 3414 3462 3608
Size: 308 1426 25 408 300 712 48 146
Cuts at: 3608 4087 5188 6218 235
Size: 479 1101 1030 2191
Fragments arranged by size:
2191 1426 1101 1030 712 479 408 308
300 146 48 25
BbeI G_GCGC'C
Cuts at: 5608 5856 5608
Size: 248 7926
BbsI GAAGACnn'nnnn_
Cuts at: 2112 2112
Size: 8174
BbvCI CC'TCA_GC
Cuts at: 898 2749 898
Size: 1851 6323
BciVI GTATCCnnnnn_n'
Cuts at: 3375 6072 7128 8174 3375
Size: 2697 1056 1046 3375
Fragments arranged by size:
3375 2697 1056 1046
BclI T'GATC_A
Cuts at: 1599 5694 1599
Size: 4095 4079
BfrBI ATG'CAT
Cuts at: 831 1290 831
Size: 459 7715
BglI GCCn_nnn'nGGC
Cuts at: 1108 1208 3950 1108
Size: 100 2742 5332
Fragments arranged by size:
5332 2742 100
BglII A'GATC_T
Cuts at: 5689 7726 5689
Size: 2037 6137
BlpI GC'TnA_GC
Cuts at: 542 1606 1786 5286 542
Size: 1064 180 3500 3430
Fragments arranged by size:
3500 3430 1064 180
Bme1580I G_kGCm'C
Cuts at: 172 688 1486 1532 1836 1969 2713 2776 3608
Size: 516 798 46 304 133 744 63 832
Cuts at: 3608 3715 4087 4120 4453 4471 4639 4769 5099
Size: 107 372 33 333 18 168 130 330
Cuts at: 5099 5799 5892 7016 172
Size: 700 93 1124 1330
Fragments arranged by size:
1330 1124 832 798 744 700 516 372
333 330 304 168 133 130 107 93
63 46 33 18
BmgBI CAC'GTC
Cuts at: 1428 3265 1428
Size: 1837 6337
BmrI ACTGGGnnnn_n'
Cuts at: 1252 2451 2687 2989 3049 3109 3969 3981 4541
Size: 1199 236 302 60 60 860 12 560
Cuts at: 4541 5386 5467 5802 7946 1252
Size: 845 81 335 2144 1480
Fragments arranged by size:
2144 1480 1199 860 845 560 335 302
236 81 60 60 12
BmtI G_CTAG'C
Cuts at: 2729 2729
Size: 8174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3384 6541 3384
Size: 3157 5017
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 395 664 1928 2239 2655 3524 5137 386
Size: 9 269 1264 311 416 869 1613 3423
Fragments arranged by size:
3423 1613 1264 869 416 311 269 9
Bpu10I CC'TnA_GC
Cuts at: 173 898 2749 173
Size: 725 1851 5598
Fragments arranged by size:
5598 1851 725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 907 2263 2503 3667 4189 4576 5350 6229
Size: 198 1356 240 1164 522 387 774 879
Cuts at: 6229 6943 7241 709
Size: 714 298 1642
Fragments arranged by size:
1642 1356 1164 879 774 714 522 387
298 240 198
BsaI GGTCTCn'nnnn_
Cuts at: 373 570 1027 1904 4066 4768 7878 373
Size: 197 457 877 2162 702 3110 669
Fragments arranged by size:
3110 2162 877 702 669 457 197
BsaAI yAC'GTr
Cuts at: 3240 5025 5527 3240
Size: 1785 502 5887
Fragments arranged by size:
5887 1785 502
BsaBI GATnn'nnATC
Cuts at: 6 5688 7725 6
Size: 5682 2037 455
Fragments arranged by size:
5682 2037 455
BsaHI Gr'CG_yC
Cuts at: 5605 5853 6555 8051 5605
Size: 248 702 1496 5728
Fragments arranged by size:
5728 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3746 3845 4223 5363 5884 6562 6973 7120 7394
Size: 99 378 1140 521 678 411 147 274
Cuts at: 7394 3746
Size: 4526
Fragments arranged by size:
4526 1140 678 521 411 378 274 147
99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 372 402 1561 1591 2397 2427 2825 2855 3178
Size: 30 1159 30 806 30 398 30 323
Cuts at: 3178 3208 372
Size: 30 5338
Fragments arranged by size:
5338 1159 806 398 323 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 481 2104 2188 2260 2440 3066 3126 3213 3511
Size: 1623 84 72 180 626 60 87 298
Cuts at: 3511 4333 481
Size: 822 4322
Fragments arranged by size:
4322 1623 822 626 298 180 87 84
72 60
BseYI C'CCAG_C
Cuts at: 384 673 747 1706 2056 2155 2160 3299 3594
Size: 289 74 959 350 99 5 1139 295
Cuts at: 3594 3957 4120 4916 5125 5599 6357 7022 7430
Size: 363 163 796 209 474 758 665 408
Cuts at: 7430 384
Size: 1128
Fragments arranged by size:
1139 1128 959 796 758 665 474 408
363 350 295 289 209 163 99 74
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 1212 2169 2599 2650 3496 4028 4075 4722
Size: 973 957 430 51 846 532 47 647
Cuts at: 4722 5131 239
Size: 409 3282
Fragments arranged by size:
3282 973 957 846 647 532 430 409
51 47
BsiEI CG_ry'CG
Cuts at: 104 1005 4959 5716 5762 6992 104
Size: 901 3954 757 46 1230 1286
Fragments arranged by size:
3954 1286 1230 901 757 46
BsiHKAI G_wGCw'C
Cuts at: 372 650 920 1355 1486 1532 1579 2843 4223
Size: 278 270 435 131 46 47 1264 1380
Cuts at: 4223 4471 4769 5099 5188 5966 6156 7016 372
Size: 248 298 330 89 778 190 860 1530
Fragments arranged by size:
1530 1380 1264 860 778 435 330 298
278 270 248 190 131 89 47 46
BsiWI C'GTAC_G
Cuts at: 5025 5025
Size: 8174
BsmI GAATG_Cn'
Cuts at: 807 807
Size: 8174
BsmBI CGTCTCn'nnnn_
Cuts at: 1418 4462 4594 1418
Size: 3044 132 4998
Fragments arranged by size:
4998 3044 132
Bsp1286I G_dGCh'C
Cuts at: 172 235 372 543 650 688 920 1355 1486
Size: 63 137 171 107 38 232 435 131
Cuts at: 1486 1532 1579 1836 1969 1994 2402 2702 2713
Size: 46 47 257 133 25 408 300 11
Cuts at: 2713 2776 2843 3414 3462 3608 3715 4087 4120
Size: 63 67 571 48 146 107 372 33
Cuts at: 4120 4223 4453 4471 4639 4769 5099 5188 5799
Size: 103 230 18 168 130 330 89 611
Cuts at: 5799 5892 5966 6156 6218 7016 172
Size: 93 74 190 62 798 1330
Fragments arranged by size:
1330 798 611 571 435 408 372 330
300 257 232 230 190 171 168 146
137 133 131 130 107 107 103 93
89 74 67 63 63 62 48 47
46 38 33 25 18 11
BspHI T'CATG_A
Cuts at: 1296 6606 1296
Size: 5310 2864
BspMI ACCTGCnnnn'nnnn_
Cuts at: 421 659 2478 2768 2898 5108 5224 5438 5740
Size: 238 1819 290 130 2210 116 214 302
Cuts at: 5740 6121 421
Size: 381 2474
Fragments arranged by size:
2474 2210 1819 381 302 290 238 214
130 116
BsrBI CCG'CTC
Cuts at: 50 4281 5146 6466 6520 8004 8169 50
Size: 4231 865 1320 54 1484 165 55
Fragments arranged by size:
4231 1484 1320 865 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3330 4899 6086 3330
Size: 1569 1187 5418
Fragments arranged by size:
5418 1569 1187
BsrFI r'CCGG_y
Cuts at: 3674 3746 3845 6172 6353 3674
Size: 72 99 2327 181 5495
Fragments arranged by size:
5495 2327 181 99 72
BssSI C'ACGA_G
Cuts at: 3688 6445 7153 3688
Size: 2757 708 4709
Fragments arranged by size:
4709 2757 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1235 1574 1782 2838 3197 3524 3794 3803 4023
Size: 339 208 1056 359 327 270 9 220
Cuts at: 4023 4051 4898 5109 5551 1235
Size: 28 847 211 442 3858
Fragments arranged by size:
3858 1056 847 442 359 339 327 270
220 211 208 28 9
BstBI TT'CG_AA
Cuts at: 6535 6535
Size: 8174
BstEII G'GTnAC_C
Cuts at: 2137 2137
Size: 8174
BstXI CCAn_nnnn'nTGG
Cuts at: 3817 3817
Size: 8174
BstYI r'GATC_y
Cuts at: 1 1304 3476 4607 5121 5199 5666 5689 6024
Size: 1303 2172 1131 514 78 467 23 335
Cuts at: 6024 6270 6576 6588 6674 7726 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2172 1303 1131 1052 514 467 449 335
306 246 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 1555 2018 2527 5162 1555
Size: 463 509 2635 4567
Fragments arranged by size:
4567 2635 509 463
BtgI C'CryG_G
Cuts at: 207 1038 1103 1893 2130 3083 3600 4162 4334
Size: 831 65 790 237 953 517 562 172
Cuts at: 4334 207
Size: 4047
Fragments arranged by size:
4047 953 831 790 562 517 237 172
65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6124 6305 6468 6124
Size: 181 163 7830
Fragments arranged by size:
7830 181 163
BtsI GCAGTG_nn'
Cuts at: 765 1137 1218 1334 2124 2625 3042 3186 3513
Size: 372 81 116 790 501 417 144 327
Cuts at: 3513 3533 3548 3584 4380 4756 4819 5417 765
Size: 20 15 36 796 376 63 598 3522
Fragments arranged by size:
3522 796 790 598 501 417 376 372
327 144 116 81 63 36 20 15
DraI TTT'AAA
Cuts at: 5173 5173
Size: 8174
DraIII CAC_nnn'GTG
Cuts at: 1508 3892 4434 4762 1508
Size: 2384 542 328 4920
Fragments arranged by size:
4920 2384 542 328
DrdI GACnn_nn'nnGTC
Cuts at: 5880 7224 5880
Size: 1344 6830
EaeI y'GGCC_r
Cuts at: 821 3783 4365 5759 5933 6324 6351 7696 821
Size: 2962 582 1394 174 391 27 1345 1299
Fragments arranged by size:
2962 1394 1345 1299 582 391 174 27
EagI C'GGCC_G
Cuts at: 5759 5759
Size: 8174
EarI CTCTTCn'nnn_
Cuts at: 302 458 479 484 589 1211 2960 6197 6407
Size: 156 21 5 105 622 1749 3237 210
Cuts at: 6407 302
Size: 2069
Fragments arranged by size:
3237 2069 1749 622 210 156 105 21
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 883 6382 7124 7270 7984 883
Size: 5499 742 146 714 1073
Fragments arranged by size:
5499 1073 742 714 146
EcoICRI GAG'CTC
Cuts at: 5186 5186
Size: 8174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 386 395 482 664 1048 1839 1928 2239
Size: 60 9 87 182 384 791 89 311
Cuts at: 2239 2384 2655 2785 3175 3266 3524 4310 4726
Size: 145 271 130 390 91 258 786 416
Cuts at: 4726 5137 5999 6431 6784 7446 326
Size: 411 862 432 353 662 1054
Fragments arranged by size:
1054 862 791 786 662 432 416 411
390 384 353 311 271 258 182 145
130 91 89 87 60 9
EcoNI CCTnn'n_nnAGG
Cuts at: 901 2245 3005 3065 3125 901
Size: 1344 760 60 60 5950
Fragments arranged by size:
5950 1344 760 60 60
EcoO109I rG'GnC_Cy
Cuts at: 220 249 906 1551 1965 2070 2202 2279 2523
Size: 29 657 645 414 105 132 77 244
Cuts at: 2523 2655 3030 3090 3150 3287 3605 4083 4084
Size: 132 375 60 60 137 318 478 1
Cuts at: 4084 4178 5313 220
Size: 94 1135 3081
Fragments arranged by size:
3081 1135 657 645 478 414 375 318
244 137 132 132 105 94 77 60
60 29 1
EcoRI G'AATT_C
Cuts at: 889 5178 889
Size: 4289 3885
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 636 2989 3063 3183 4854 6193 636
Size: 2353 74 120 1671 1339 2617
Fragments arranged by size:
2617 2353 1671 1339 120 74
FspI TGC'GCA
Cuts at: 4849 5955 4849
Size: 1106 7068
HaeII r_GCGC'y
Cuts at: 618 5608 5856 7086 7857 618
Size: 4990 248 1230 771 935
Fragments arranged by size:
4990 1230 935 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 222 254 372 404 550 582 1278 1310 2206
Size: 32 118 32 146 32 696 32 896
Cuts at: 2206 2238 2494 2526 4490 4522 4767 4799 6016
Size: 32 256 32 1964 32 245 32 1217
Cuts at: 6016 6046 6048 6078 222
Size: 30 2 30 2318
Fragments arranged by size:
2318 1964 1217 896 696 256 245 146
118 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2097 3936 4668 5213 2097
Size: 1839 732 545 5058
Fragments arranged by size:
5058 1839 732 545
KasI G'GCGC_C
Cuts at: 5604 5852 5604
Size: 248 7926
KpnI G_GTAC'C
Cuts at: 2852 3747 3824 5194 2852
Size: 895 77 1370 5832
Fragments arranged by size:
5832 1370 895 77
MluI A'CGCG_T
Cuts at: 7607 7607
Size: 8174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 869 1383 1487 6933 7117 869
Size: 514 104 5446 184 1926
Fragments arranged by size:
5446 1926 514 184 104
MscI TGG'CCA
Cuts at: 823 4367 5935 823
Size: 3544 1568 3062
Fragments arranged by size:
3544 3062 1568
MslI CAynn'nnrTG
Cuts at: 785 1295 1379 1397 1898 2135 4716 5102 6163
Size: 510 84 18 501 237 2581 386 1061
Cuts at: 6163 6290 785
Size: 127 2669
Fragments arranged by size:
2669 2581 1061 510 501 386 237 127
84 18
MspA1I CmG'CkG
Cuts at: 460 546 1605 2004 2183 2618 3941 4103 4250
Size: 86 1059 399 179 435 1323 162 147
Cuts at: 4250 4416 4674 4916 5115 5285 5599 5959 6743
Size: 166 258 242 199 170 314 360 784
Cuts at: 6743 6988 460
Size: 245 1646
Fragments arranged by size:
1646 1323 1059 784 435 399 360 314
258 245 242 199 179 170 166 162
147 86
NaeI GCC'GGC
Cuts at: 6355 6355
Size: 8174
NarI GG'CG_CC
Cuts at: 5605 5853 5605
Size: 248 7926
NcoI C'CATG_G
Cuts at: 1038 1103 1893 2130 1038
Size: 65 790 237 7082
Fragments arranged by size:
7082 790 237 65
NdeI CA'TA_TG
Cuts at: 596 596
Size: 8174
NgoMIV G'CCGG_C
Cuts at: 6353 6353
Size: 8174
NheI G'CTAG_C
Cuts at: 2725 2725
Size: 8174
NsiI A_TGCA'T
Cuts at: 833 1292 833
Size: 459 7715
NspI r_CATG'y
Cuts at: 831 1294 1368 1421 1452 1482 1536 1546 3434
Size: 463 74 53 31 30 54 10 1888
Cuts at: 3434 3666 4021 4406 4605 5227 5727 7330 831
Size: 232 355 385 199 622 500 1603 1675
Fragments arranged by size:
1888 1675 1603 622 500 463 385 355
232 199 74 54 53 31 30 10
PasI CC'CwG_GG
Cuts at: 2205 2283 2945 2205
Size: 78 662 7434
Fragments arranged by size:
7434 662 78
PciI A'CATG_T
Cuts at: 1478 3430 7326 1478
Size: 1952 3896 2326
Fragments arranged by size:
3896 2326 1952
PflMI CCAn_nnn'nTGG
Cuts at: 3601 4416 5067 3601
Size: 815 651 6708
Fragments arranged by size:
6708 815 651
PfoI T'CCnGG_A
Cuts at: 372 402 2671 372
Size: 30 2269 5875
Fragments arranged by size:
5875 2269 30
PmeI GTTT'AAAC
Cuts at: 5173 5173
Size: 8174
PmlI CAC'GTG
Cuts at: 3240 3240
Size: 8174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6620 6652 6620
Size: 32 8142
PpuMI rG'GwC_Cy
Cuts at: 249 1551 3287 249
Size: 1302 1736 5136
Fragments arranged by size:
5136 1736 1302
PspOMI G'GGCC_C
Cuts at: 1965 3604 4083 1965
Size: 1639 479 6056
Fragments arranged by size:
6056 1639 479
PspXI vC'TCGA_Gb
Cuts at: 3493 3493
Size: 8174
PstI C_TGCA'G
Cuts at: 988 1691 1756 3009 3069 3129 3256 3520 3591
Size: 703 65 1253 60 60 127 264 71
Cuts at: 3591 3799 4071 5221 5906 988
Size: 208 272 1150 685 3256
Fragments arranged by size:
3256 1253 1150 703 685 272 264 208
127 71 65 60 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8174
PvuII CAG'CTG
Cuts at: 460 1605 2004 2183 3941 4416 4674 5115 5599
Size: 1145 399 179 1758 475 258 441 484
Cuts at: 5599 5959 460
Size: 360 2675
Fragments arranged by size:
2675 1758 1145 484 475 441 399 360
258 179
RsrII CG'GwC_CG
Cuts at: 6369 6369
Size: 8174
SacI G_AGCT'C
Cuts at: 5188 5188
Size: 8174
SalI G'TCGA_C
Cuts at: 5211 5211
Size: 8174
SanDI GG'GwC_CC
Cuts at: 249 3287 249
Size: 3038 5136
SapI GCTCTTCn'nnn_
Cuts at: 1211 2960 6197 6407 1211
Size: 1749 3237 210 2978
Fragments arranged by size:
3237 2978 1749 210
SbfI CC_TGCA'GG
Cuts at: 3009 3069 3129 5221 3009
Size: 60 60 2092 5962
Fragments arranged by size:
5962 2092 60 60
ScaI AGT'ACT
Cuts at: 4735 7691 4735
Size: 2956 5218
SfcI C'TryA_G
Cuts at: 33 783 984 1687 1752 3005 3065 3125 3252
Size: 750 201 703 65 1253 60 60 127
Cuts at: 3252 3516 3587 3619 3795 3897 4067 5217 5902
Size: 264 71 32 176 102 170 1150 685
Cuts at: 5902 6870 7061 33
Size: 968 191 1146
Fragments arranged by size:
1253 1150 1146 968 750 703 685 264
201 191 176 170 127 102 71 65
60 60 32
SfoI GGC'GCC
Cuts at: 5606 5854 5606
Size: 248 7926
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8174
SgrAI Cr'CCGG_yG
Cuts at: 3674 3674
Size: 8174
SmaI CCC'GGG
Cuts at: 247 4276 5196 247
Size: 4029 920 3225
Fragments arranged by size:
4029 3225 920
SmlI C'TyrA_G
Cuts at: 724 922 2242 2518 3493 3555 3682 4009 4168
Size: 198 1320 276 975 62 127 327 159
Cuts at: 4168 4591 5329 6244 6958 7220 724
Size: 423 738 915 714 262 1678
Fragments arranged by size:
1678 1320 975 915 738 714 423 327
276 262 198 159 127 62
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8174
SphI G_CATG'C
Cuts at: 831 1294 3666 4021 5227 5727 831
Size: 463 2372 355 1206 500 3278
Fragments arranged by size:
3278 2372 1206 500 463 355
StuI AGG'CCT
Cuts at: 1012 1918 2857 4192 4825 1012
Size: 906 939 1335 633 4361
Fragments arranged by size:
4361 1335 939 906 633
StyI C'CwwG_G
Cuts at: 534 1038 1103 1469 1893 2130 2685 3948 5308
Size: 504 65 366 424 237 555 1263 1360
Cuts at: 5308 5662 534
Size: 354 3046
Fragments arranged by size:
3046 1360 1263 555 504 424 366 354
237 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 514 739 2134 2347 2669 2770 2835 3096 3156
Size: 225 1395 213 322 101 65 261 60
Cuts at: 3156 4175 4867 4963 5757 5890 6349 6558 514
Size: 1019 692 96 794 133 459 209 2130
Fragments arranged by size:
2130 1395 1019 794 692 459 322 261
225 213 209 133 101 96 65 60
TatI w'GTAC_w
Cuts at: 3355 4733 7689 3355
Size: 1378 2956 3840
Fragments arranged by size:
3840 2956 1378
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 151 183 1465 1497 1687 1719 1859 1891 2397
Size: 32 1282 32 190 32 140 32 506
Cuts at: 2397 2429 3178 3210 7691 7723 151
Size: 32 749 32 4481 32 602
Fragments arranged by size:
4481 1282 749 602 506 190 140 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3055 3115 5971 3055
Size: 60 2856 5258
Fragments arranged by size:
5258 2856 60
XbaI T'CTAG_A
Cuts at: 5205 5205
Size: 8174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1714 3622 5669 1714
Size: 1908 2047 4219
Fragments arranged by size:
4219 2047 1908
XhoI C'TCGA_G
Cuts at: 3493 3493
Size: 8174
XmaI C'CCGG_G
Cuts at: 245 4274 5194 245
Size: 4029 920 3225
Fragments arranged by size:
4029 3225 920
XmnI GAAnn'nnTTC
Cuts at: 703 703
Size: 8174
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AloI AscI AvrII BcgI BspEI BsrGI
BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NotI NruI PacI PshAI PsiI PsrI
SacII SexAI SfiI SnaBI SrfI SspI SwaI ZraI