Restriction map for
pF1KA0231
(Circular) (Six-base) MAPSORT of: pF1KA0231.seq Check: 3164 from: 1 to: 5522
pF1KA0231 5522 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 185 185
Size: 5522
AccI GT'mk_AC
Cuts at: 1538 2560 1538
Size: 1022 4500
Acc65I G'GTAC_C
Cuts at: 2538 2538
Size: 5522
AclI AA'CG_TT
Cuts at: 2490 5362 2490
Size: 2872 2650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1987 3347 3779 4132 4794 1153
Size: 834 1360 432 353 662 1881
Fragments arranged by size:
1881 1360 834 662 432 353
AfeI AGC'GCT
Cuts at: 1009 1009
Size: 5522
AflIII A'CryG_T
Cuts at: 2874 4674 4955 2874
Size: 1800 281 3441
Fragments arranged by size:
3441 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2243 2302 2243
Size: 59 5463
AlwNI CAG_nnn'CTG
Cuts at: 628 1803 2208 2827 4265 628
Size: 1175 405 619 1438 1885
Fragments arranged by size:
1885 1438 1175 619 405
ApaLI G'TGCA_C
Cuts at: 4360 4360
Size: 5522
ApoI r'AATT_y
Cuts at: 289 383 1569 1591 2322 2526 4875 5131 289
Size: 94 1186 22 731 204 2349 256 680
Fragments arranged by size:
2349 1186 731 680 256 204 94 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 5522
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
AvaI C'yCGr_G
Cuts at: 553 2542 553
Size: 1989 3533
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 522 555 522
Size: 33 5489
BamHI G'GATC_C
Cuts at: 1760 2547 1760
Size: 787 4735
BanI G'GyrC_C
Cuts at: 2538 2952 3200 3235 2538
Size: 414 248 35 4825
Fragments arranged by size:
4825 414 248 35
BanII G_rGCy'C
Cuts at: 240 2372 2536 3566 240
Size: 2132 164 1030 2196
Fragments arranged by size:
2196 2132 1030 164
BbeI G_GCGC'C
Cuts at: 2956 3204 2956
Size: 248 5274
BbsI GAAGACnn'nnnn_
Cuts at: 177 265 1393 177
Size: 88 1128 4306
Fragments arranged by size:
4306 1128 88
BbvCI CC'TCA_GC
Cuts at: 1120 1120
Size: 5522
BciVI GTATCCnnnnn_n'
Cuts at: 3420 4476 5522 3420
Size: 1056 1046 3420
Fragments arranged by size:
3420 1056 1046
BclI T'GATC_A
Cuts at: 206 1227 1883 3042 206
Size: 1021 656 1159 2686
Fragments arranged by size:
2686 1159 1021 656
BfrBI ATG'CAT
Cuts at: 846 846
Size: 5522
BglI GCCn_nnn'nGGC
Cuts at: 229 229
Size: 5522
BglII A'GATC_T
Cuts at: 3037 5074 3037
Size: 2037 3485
BlpI GC'TnA_GC
Cuts at: 135 629 1441 2634 135
Size: 494 812 1193 3023
Fragments arranged by size:
3023 1193 812 494
Bme1580I G_kGCm'C
Cuts at: 3147 3240 4364 3147
Size: 93 1124 4305
Fragments arranged by size:
4305 1124 93
BmgBI CAC'GTC
Cuts at: 2501 2501
Size: 5522
BmrI ACTGGGnnnn_n'
Cuts at: 141 530 2734 2815 3150 5294 141
Size: 389 2204 81 335 2144 369
Fragments arranged by size:
2204 2144 389 369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2106 3889 2106
Size: 1783 3739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1468 2386 226
Size: 1242 918 3362
Fragments arranged by size:
3362 1242 918
Bpu10I CC'TnA_GC
Cuts at: 1120 1120
Size: 5522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 1618 1627 2698 3577 4291 4589 1486
Size: 132 9 1071 879 714 298 2419
Fragments arranged by size:
2419 1071 879 714 298 132 9
BsaI GGTCTCn'nnnn_
Cuts at: 5226 5226
Size: 5522
BsaAI yAC'GTr
Cuts at: 2875 2875
Size: 5522
BsaBI GATnn'nnATC
Cuts at: 6 3036 5073 6
Size: 3030 2037 455
Fragments arranged by size:
3030 2037 455
BsaHI Gr'CG_yC
Cuts at: 182 2953 3201 3903 5399 182
Size: 2771 248 702 1496 305
Fragments arranged by size:
2771 1496 702 305 248
BsaWI w'CCGG_w
Cuts at: 2711 3232 3910 4321 4468 4742 2711
Size: 521 678 411 147 274 3491
Fragments arranged by size:
3491 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2382 2412 2382
Size: 30 5492
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1137 1579 2464 1137
Size: 442 885 4195
Fragments arranged by size:
4195 885 442
BseYI C'CCAG_C
Cuts at: 1619 2947 3705 4370 4778 1619
Size: 1328 758 665 408 2363
Fragments arranged by size:
2363 1328 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1532 2039 1006
Size: 526 507 4489
Fragments arranged by size:
4489 526 507
BsiEI CG_ry'CG
Cuts at: 104 3064 3110 4340 104
Size: 2960 46 1230 1286
Fragments arranged by size:
2960 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 240 485 920 2372 2536 3314 3504 4364 240
Size: 245 435 1452 164 778 190 860 1398
Fragments arranged by size:
1452 1398 860 778 435 245 190 164
Bsp1286I G_dGCh'C
Cuts at: 240 485 920 2372 2536 3147 3240 3314 3504
Size: 245 435 1452 164 611 93 74 190
Cuts at: 3504 3566 4364 240
Size: 62 798 1398
Fragments arranged by size:
1452 1398 798 611 435 245 190 164
93 74 62
BspHI T'CATG_A
Cuts at: 3954 3954
Size: 5522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2572 2786 3088 3469 2572
Size: 214 302 381 4625
Fragments arranged by size:
4625 381 302 214
BsrBI CCG'CTC
Cuts at: 50 372 3814 3868 5352 5517 50
Size: 322 3442 54 1484 165 55
Fragments arranged by size:
3442 1484 322 165 55 54
BsrDI GCAATG_nn'
Cuts at: 721 1792 2039 3434 721
Size: 1071 247 1395 2809
Fragments arranged by size:
2809 1395 1071 247
BsrFI r'CCGG_y
Cuts at: 3520 3701 3520
Size: 181 5341
BssSI C'ACGA_G
Cuts at: 3793 4501 3793
Size: 708 4814
BstAPI GCAn_nnn'nTGC
Cuts at: 2899 2899
Size: 5522
BstBI TT'CG_AA
Cuts at: 3883 3883
Size: 5522
BstXI CCAn_nnnn'nTGG
Cuts at: 783 783
Size: 5522
BstYI r'GATC_y
Cuts at: 1 1760 2160 2547 3014 3037 3372 3618 3924
Size: 1759 400 387 467 23 335 246 306
Cuts at: 3924 3936 4022 5074 1
Size: 12 86 1052 449
Fragments arranged by size:
1759 1052 467 449 400 387 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 684 1951 684
Size: 1267 4255
BtgI C'CryG_G
Cuts at: 172 595 172
Size: 423 5099
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3472 3653 3816 3472
Size: 181 163 5178
Fragments arranged by size:
5178 181 163
BtsI GCAGTG_nn'
Cuts at: 2296 2765 2296
Size: 469 5053
DraI TTT'AAA
Cuts at: 1574 2521 1574
Size: 947 4575
DrdI GACnn_nn'nnGTC
Cuts at: 3228 4572 3228
Size: 1344 4178
EaeI y'GGCC_r
Cuts at: 221 230 566 3107 3281 3672 3699 5044 221
Size: 9 336 2541 174 391 27 1345 699
Fragments arranged by size:
2541 1345 699 391 336 174 27 9
EagI C'GGCC_G
Cuts at: 3107 3107
Size: 5522
EarI CTCTTCn'nnn_
Cuts at: 1101 1738 1973 2474 3545 3755 1101
Size: 637 235 501 1071 210 2868
Fragments arranged by size:
2868 1071 637 501 235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1573 3730 4472 4618 5332 1573
Size: 2157 742 146 714 1763
Fragments arranged by size:
2157 1763 742 714 146
EcoICRI GAG'CTC
Cuts at: 238 2370 2534 238
Size: 2132 164 3226
Fragments arranged by size:
3226 2132 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1153 1468 1987 2386 3347 3779 4132 4794
Size: 927 315 519 399 961 432 353 662
Cuts at: 4794 226
Size: 954
Fragments arranged by size:
961 954 927 662 519 432 399 353
315
EcoNI CCTnn'n_nnAGG
Cuts at: 1273 1273
Size: 5522
EcoO109I rG'GnC_Cy
Cuts at: 1047 1730 2661 1047
Size: 683 931 3908
Fragments arranged by size:
3908 931 683
EcoRI G'AATT_C
Cuts at: 383 2526 383
Size: 2143 3379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 788 1218 3541 788
Size: 430 2323 2769
Fragments arranged by size:
2769 2323 430
FspI TGC'GCA
Cuts at: 3303 3303
Size: 5522
HaeII r_GCGC'y
Cuts at: 1011 2956 3204 4434 5205 1011
Size: 1945 248 1230 771 1328
Fragments arranged by size:
1945 1328 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 1848 1880 3364 3394 3396 3426 133
Size: 32 1683 32 1484 30 2 30 2229
Fragments arranged by size:
2229 1683 1484 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1497 2561 1424
Size: 73 1064 4385
Fragments arranged by size:
4385 1064 73
HindIII A'AGCT_T
Cuts at: 1345 1345
Size: 5522
HpaI GTT'AAC
Cuts at: 1424 1424
Size: 5522
KasI G'GCGC_C
Cuts at: 2952 3200 2952
Size: 248 5274
KpnI G_GTAC'C
Cuts at: 2542 2542
Size: 5522
MluI A'CGCG_T
Cuts at: 4955 4955
Size: 5522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1679 4281 4465 1679
Size: 2602 184 2736
Fragments arranged by size:
2736 2602 184
MscI TGG'CCA
Cuts at: 568 3283 568
Size: 2715 2807
MslI CAynn'nnrTG
Cuts at: 847 2339 3511 3638 847
Size: 1492 1172 127 2731
Fragments arranged by size:
2731 1492 1172 127
MspA1I CmG'CkG
Cuts at: 246 697 754 1491 1889 2633 2947 3307 4091
Size: 451 57 737 398 744 314 360 784
Cuts at: 4091 4336 246
Size: 245 1432
Fragments arranged by size:
1432 784 744 737 451 398 360 314
245 57
NaeI GCC'GGC
Cuts at: 3703 3703
Size: 5522
NarI GG'CG_CC
Cuts at: 2953 3201 2953
Size: 248 5274
NcoI C'CATG_G
Cuts at: 172 595 172
Size: 423 5099
NgoMIV G'CCGG_C
Cuts at: 3701 3701
Size: 5522
NsiI A_TGCA'T
Cuts at: 848 848
Size: 5522
NspI r_CATG'y
Cuts at: 846 850 2575 3075 4678 846
Size: 4 1725 500 1603 1690
Fragments arranged by size:
1725 1690 1603 500 4
PciI A'CATG_T
Cuts at: 4674 4674
Size: 5522
PfoI T'CCnGG_A
Cuts at: 782 1756 782
Size: 974 4548
PmeI GTTT'AAAC
Cuts at: 2521 2521
Size: 5522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 331 363 3968 4000 331
Size: 32 3605 32 1853
Fragments arranged by size:
3605 1853 32 32
PpuMI rG'GwC_Cy
Cuts at: 1047 1730 1047
Size: 683 4839
PspXI vC'TCGA_Gb
Cuts at: 553 553
Size: 5522
PstI C_TGCA'G
Cuts at: 514 1482 2291 2569 3254 514
Size: 968 809 278 685 2782
Fragments arranged by size:
2782 968 809 685 278
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5522
PvuII CAG'CTG
Cuts at: 246 697 754 1491 1889 2947 3307 246
Size: 451 57 737 398 1058 360 2461
Fragments arranged by size:
2461 1058 737 451 398 360 57
RsrII CG'GwC_CG
Cuts at: 3717 3717
Size: 5522
SacI G_AGCT'C
Cuts at: 240 2372 2536 240
Size: 2132 164 3226
Fragments arranged by size:
3226 2132 164
SalI G'TCGA_C
Cuts at: 2559 2559
Size: 5522
SapI GCTCTTCn'nnn_
Cuts at: 1738 3545 3755 1738
Size: 1807 210 3505
Fragments arranged by size:
3505 1807 210
SbfI CC_TGCA'GG
Cuts at: 2569 2569
Size: 5522
ScaI AGT'ACT
Cuts at: 412 1689 2224 5039 412
Size: 1277 535 2815 895
Fragments arranged by size:
2815 1277 895 535
SfcI C'TryA_G
Cuts at: 33 510 1101 1173 1478 2287 2565 3250 4218
Size: 477 591 72 305 809 278 685 968
Cuts at: 4218 4409 33
Size: 191 1146
Fragments arranged by size:
1146 968 809 685 591 477 305 278
191 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 229 229
Size: 5522
SfoI GGC'GCC
Cuts at: 2954 3202 2954
Size: 248 5274
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
SmaI CCC'GGG
Cuts at: 2544 2544
Size: 5522
SmlI C'TyrA_G
Cuts at: 553 1501 1633 1642 2677 3592 4306 4568 553
Size: 948 132 9 1035 915 714 262 1507
Fragments arranged by size:
1507 1035 948 915 714 262 132 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5522
SphI G_CATG'C
Cuts at: 846 2575 3075 846
Size: 1729 500 3293
Fragments arranged by size:
3293 1729 500
SspI AAT'ATT
Cuts at: 1141 2109 2247 1141
Size: 968 138 4416
Fragments arranged by size:
4416 968 138
StuI AGG'CCT
Cuts at: 645 645
Size: 5522
StyI C'CwwG_G
Cuts at: 172 313 595 1028 2656 3010 172
Size: 141 282 433 1628 354 2684
Fragments arranged by size:
2684 1628 433 354 282 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2329 3105 3238 3697 3906 2329
Size: 776 133 459 209 3945
Fragments arranged by size:
3945 776 459 209 133
TatI w'GTAC_w
Cuts at: 410 1687 1736 2222 5037 410
Size: 1277 49 486 2815 895
Fragments arranged by size:
2815 1277 895 486 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5039 5071 5039
Size: 32 5490
Tth111I GACn'n_nGTC
Cuts at: 1411 3319 1411
Size: 1908 3614
XbaI T'CTAG_A
Cuts at: 1560 2553 1560
Size: 993 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3017 3017
Size: 5522
XhoI C'TCGA_G
Cuts at: 553 553
Size: 5522
XmaI C'CCGG_G
Cuts at: 2542 2542
Size: 5522
XmnI GAAnn'nnTTC
Cuts at: 835 2393 835
Size: 1558 3964
ZraI GAC'GTC
Cuts at: 183 183
Size: 5522
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AleI AloI ApaI AscI
AvrII BcgI BmtI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SwaI