Restriction map for
pF1KA0228
(Circular) (Six-base) MAPSORT of: pF1KA0228.seq Check: 5964 from: 1 to: 4868
pF1KA0228 4868 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 1906 1906
Size: 4868
Acc65I G'GTAC_C
Cuts at: 1884 1884
Size: 4868
AclI AA'CG_TT
Cuts at: 4708 4708
Size: 4868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 532 1235 1472 1829 2693 3125 3872 4140
Size: 158 703 237 357 864 432 747 268
Cuts at: 4140 374
Size: 1102
Fragments arranged by size:
1102 864 747 703 432 357 268 237
158
AflII C'TTAA_G
Cuts at: 852 852
Size: 4868
AflIII A'CryG_T
Cuts at: 2220 3324 4301 2220
Size: 1104 977 2787
Fragments arranged by size:
2787 1104 977
AgeI A'CCGG_T
Cuts at: 1739 3256 1739
Size: 1517 3351
AlwNI CAG_nnn'CTG
Cuts at: 445 1311 2173 3740 445
Size: 866 862 1567 1573
Fragments arranged by size:
1573 1567 866 862
ApaLI G'TGCA_C
Cuts at: 746 3638 746
Size: 2892 1976
ApoI r'AATT_y
Cuts at: 283 889 1153 1375 1438 1872 4221 4477 283
Size: 606 264 222 63 434 2349 256 674
Fragments arranged by size:
2349 674 606 434 264 256 222 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 4868
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
AvaI C'yCGr_G
Cuts at: 214 1888 214
Size: 1674 3194
BamHI G'GATC_C
Cuts at: 131 949 1893 131
Size: 818 944 3106
Fragments arranged by size:
3106 944 818
BanI G'GyrC_C
Cuts at: 1884 2298 2546 2581 1884
Size: 414 248 35 4171
Fragments arranged by size:
4171 414 248 35
BanII G_rGCy'C
Cuts at: 1882 2912 1882
Size: 1030 3838
BbeI G_GCGC'C
Cuts at: 2302 2550 2302
Size: 248 4620
BbsI GAAGACnn'nnnn_
Cuts at: 1334 1422 1334
Size: 88 4780
BciVI GTATCCnnnnn_n'
Cuts at: 2766 3527 4868 2766
Size: 761 1341 2766
Fragments arranged by size:
2766 1341 761
BclI T'GATC_A
Cuts at: 2388 2388
Size: 4868
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4868
BglII A'GATC_T
Cuts at: 2383 4420 2383
Size: 2037 2831
BlpI GC'TnA_GC
Cuts at: 1980 1980
Size: 4868
Bme1580I G_kGCm'C
Cuts at: 750 2493 2586 3642 750
Size: 1743 93 1056 1976
Fragments arranged by size:
1976 1743 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 283 1794 2080 2161 2496 4640 283
Size: 1511 286 81 335 2144 511
Fragments arranged by size:
2144 1511 511 335 286 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3235 3235
Size: 4868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2044 2923 3415 3713 2044
Size: 879 492 298 3199
Fragments arranged by size:
3199 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1669 4572 1669
Size: 2903 1965
BsaAI yAC'GTr
Cuts at: 1092 2221 1092
Size: 1129 3739
BsaBI GATnn'nnATC
Cuts at: 6 2382 4419 6
Size: 2376 2037 455
Fragments arranged by size:
2376 2037 455
BsaHI Gr'CG_yC
Cuts at: 2299 2547 3249 4745 2299
Size: 248 702 1496 2422
Fragments arranged by size:
2422 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1739 2057 2578 3256 3530 3677 4088 1739
Size: 318 521 678 274 147 411 2519
Fragments arranged by size:
2519 678 521 411 318 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 172 190 202 220 172
Size: 18 12 18 4820
Fragments arranged by size:
4820 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1282 1282
Size: 4868
BseYI C'CCAG_C
Cuts at: 2293 3051 3628 4124 2293
Size: 758 577 496 3037
Fragments arranged by size:
3037 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 710 1840 611
Size: 99 1130 3639
Fragments arranged by size:
3639 1130 99
BsiEI CG_ry'CG
Cuts at: 104 116 2410 2456 3664 104
Size: 12 2294 46 1208 1308
Fragments arranged by size:
2294 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 424 750 1882 2660 2850 3642 424
Size: 326 1132 778 190 792 1650
Fragments arranged by size:
1650 1132 792 778 326 190
Bsp1286I G_dGCh'C
Cuts at: 424 750 1882 2493 2586 2660 2850 2912 3642
Size: 326 1132 611 93 74 190 62 730
Cuts at: 3642 424
Size: 1650
Fragments arranged by size:
1650 1132 730 611 326 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4088 4088
Size: 4868
BspHI T'CATG_A
Cuts at: 1104 1326 4044 1104
Size: 222 2718 1928
Fragments arranged by size:
2718 1928 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1918 2132 2434 2815 1918
Size: 214 302 381 3971
Fragments arranged by size:
3971 381 302 214
BsrBI CCG'CTC
Cuts at: 50 188 206 3160 3214 4698 4863 50
Size: 138 18 2954 54 1484 165 55
Fragments arranged by size:
2954 1484 165 138 55 54 18
BsrDI GCAATG_nn'
Cuts at: 809 2780 809
Size: 1971 2897
BsrFI r'CCGG_y
Cuts at: 1739 2866 3047 3256 1739
Size: 1127 181 209 3351
Fragments arranged by size:
3351 1127 209 181
BsrGI T'GTAC_A
Cuts at: 1383 1383
Size: 4868
BssSI C'ACGA_G
Cuts at: 3139 3497 3139
Size: 358 4510
BstAPI GCAn_nnn'nTGC
Cuts at: 2245 2245
Size: 4868
BstBI TT'CG_AA
Cuts at: 3229 3229
Size: 4868
BstYI r'GATC_y
Cuts at: 1 131 949 1893 2360 2383 2718 2964 3976
Size: 130 818 944 467 23 335 246 1012
Cuts at: 3976 4062 4074 4420 1
Size: 86 12 346 449
Fragments arranged by size:
1012 944 818 467 449 346 335 246
130 86 23 12
BtgI C'CryG_G
Cuts at: 107 116 107
Size: 9 4859
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2818 2999 3162 2818
Size: 181 163 4524
Fragments arranged by size:
4524 181 163
BtsI GCAGTG_nn'
Cuts at: 285 929 976 2111 285
Size: 644 47 1135 3042
Fragments arranged by size:
3042 1135 644 47
DraI TTT'AAA
Cuts at: 666 1867 666
Size: 1201 3667
DrdI GACnn_nn'nnGTC
Cuts at: 266 2574 3432 266
Size: 2308 858 1702
Fragments arranged by size:
2308 1702 858
EaeI y'GGCC_r
Cuts at: 113 705 2453 2627 3018 3045 4390 113
Size: 592 1748 174 391 27 1345 591
Fragments arranged by size:
1748 1345 592 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 2453 113
Size: 2340 2528
EarI CTCTTCn'nnn_
Cuts at: 1128 2891 3101 1128
Size: 1763 210 2895
Fragments arranged by size:
2895 1763 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3076 3386 3532 4678 3076
Size: 310 146 1146 3266
Fragments arranged by size:
3266 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1880 1880
Size: 4868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 532 1235 1472 1829 2693 3125 3872 4140
Size: 158 703 237 357 864 432 747 268
Cuts at: 4140 374
Size: 1102
Fragments arranged by size:
1102 864 747 703 432 357 268 237
158
EcoO109I rG'GnC_Cy
Cuts at: 2007 2007
Size: 4868
EcoRI G'AATT_C
Cuts at: 1872 1872
Size: 4868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 878 1240 1507 1537 2887 878
Size: 362 267 30 1350 2859
Fragments arranged by size:
2859 1350 362 267 30
FspI TGC'GCA
Cuts at: 2649 2649
Size: 4868
HaeII r_GCGC'y
Cuts at: 2302 2550 3572 4551 2302
Size: 248 1022 979 2619
Fragments arranged by size:
2619 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 679 711 1758 1790 2710 2740 2742
Size: 32 457 32 1047 32 920 30 2
Cuts at: 2742 2772 190
Size: 30 2286
Fragments arranged by size:
2286 1047 920 457 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 271 1907 271
Size: 1636 3232
HindIII A'AGCT_T
Cuts at: 244 860 1364 244
Size: 616 504 3748
Fragments arranged by size:
3748 616 504
KasI G'GCGC_C
Cuts at: 2298 2546 2298
Size: 248 4620
KpnI G_GTAC'C
Cuts at: 1888 1888
Size: 4868
MfeI C'AATT_G
Cuts at: 1277 1607 1277
Size: 330 4538
MluI A'CGCG_T
Cuts at: 4301 4301
Size: 4868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1374 3539 3723 1374
Size: 2165 184 2519
Fragments arranged by size:
2519 2165 184
MscI TGG'CCA
Cuts at: 707 2629 707
Size: 1922 2946
MslI CAynn'nnrTG
Cuts at: 650 773 786 2857 2984 650
Size: 123 13 2071 127 2534
Fragments arranged by size:
2534 2071 127 123 13
MspA1I CmG'CkG
Cuts at: 166 1979 2293 2653 3666 3911 166
Size: 1813 314 360 1013 245 1123
Fragments arranged by size:
1813 1123 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3049 3049
Size: 4868
NarI GG'CG_CC
Cuts at: 2299 2547 2299
Size: 248 4620
NcoI C'CATG_G
Cuts at: 107 107
Size: 4868
NgoMIV G'CCGG_C
Cuts at: 3047 3047
Size: 4868
NspI r_CATG'y
Cuts at: 922 1921 2421 3328 922
Size: 999 500 907 2462
Fragments arranged by size:
2462 999 907 500
PacI TTA_AT'TAA
Cuts at: 914 914
Size: 4868
PciI A'CATG_T
Cuts at: 3324 3324
Size: 4868
PmeI GTTT'AAAC
Cuts at: 1867 1867
Size: 4868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 4007
Size: 32 4836
PstI C_TGCA'G
Cuts at: 450 1915 2600 450
Size: 1465 685 2718
Fragments arranged by size:
2718 1465 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4868
PvuII CAG'CTG
Cuts at: 2293 2653 2293
Size: 360 4508
RsrII CG'GwC_CG
Cuts at: 3063 3063
Size: 4868
SacI G_AGCT'C
Cuts at: 1882 1882
Size: 4868
SalI G'TCGA_C
Cuts at: 1905 1905
Size: 4868
SapI GCTCTTCn'nnn_
Cuts at: 2891 3101 2891
Size: 210 4658
SbfI CC_TGCA'GG
Cuts at: 1915 1915
Size: 4868
ScaI AGT'ACT
Cuts at: 314 4385 314
Size: 4071 797
SfcI C'TryA_G
Cuts at: 33 446 1601 1635 1911 2596 3589 3780 33
Size: 413 1155 34 276 685 993 191 1121
Fragments arranged by size:
1155 1121 993 685 413 276 191 34
SfoI GGC'GCC
Cuts at: 2300 2548 2300
Size: 248 4620
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
SmaI CCC'GGG
Cuts at: 1890 1890
Size: 4868
SmlI C'TyrA_G
Cuts at: 852 2023 2938 3430 3692 852
Size: 1171 915 492 262 2028
Fragments arranged by size:
2028 1171 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4868
SphI G_CATG'C
Cuts at: 1921 2421 1921
Size: 500 4368
SspI AAT'ATT
Cuts at: 967 1759 967
Size: 792 4076
StyI C'CwwG_G
Cuts at: 107 1410 2002 2356 107
Size: 1303 592 354 2619
Fragments arranged by size:
2619 1303 592 354
SwaI ATTT'AAAT
Cuts at: 666 666
Size: 4868
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 640 974 1869 2451 2584 3043 3252 640
Size: 334 895 582 133 459 209 2256
Fragments arranged by size:
2256 895 582 459 334 209 133
TatI w'GTAC_w
Cuts at: 312 556 1383 4383 312
Size: 244 827 3000 797
Fragments arranged by size:
3000 827 797 244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 112 144 4385 4417 112
Size: 32 4241 32 563
Fragments arranged by size:
4241 563 32 32
Tth111I GACn'n_nGTC
Cuts at: 2665 2665
Size: 4868
XbaI T'CTAG_A
Cuts at: 1899 1899
Size: 4868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1144 1812 2363 114
Size: 1030 668 551 2619
Fragments arranged by size:
2619 1030 668 551
XmaI C'CCGG_G
Cuts at: 1888 1888
Size: 4868
XmnI GAAnn'nnTTC
Cuts at: 1490 1490
Size: 4868
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PacI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI XhoI ZraI