Restriction map for
pF1KA0226
(Circular) (Six-base) MAPSORT of: pF1KA0226.seq Check: 7210 from: 1 to: 5894
pF1KA0226 5894 bp
With 182 enzymes: SgfI *
October 20, 2015 16:07 ..
AccI GT'mk_AC
Cuts at: 2932 2932
Size: 5894
Acc65I G'GTAC_C
Cuts at: 1340 2910 1340
Size: 1570 4324
AclI AA'CG_TT
Cuts at: 5734 5734
Size: 5894
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1443 1597 1894 2259 3719 4151 4898 5166 1443
Size: 154 297 365 1460 432 747 268 2171
Fragments arranged by size:
2171 1460 747 432 365 297 268 154
AfeI AGC'GCT
Cuts at: 2855 2855
Size: 5894
AflIII A'CryG_T
Cuts at: 2435 3246 4350 5327 2435
Size: 811 1104 977 3002
Fragments arranged by size:
3002 1104 977 811
AgeI A'CCGG_T
Cuts at: 4282 4282
Size: 5894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1254 1254
Size: 5894
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1348 1380 1348
Size: 32 5862
AlwNI CAG_nnn'CTG
Cuts at: 394 441 546 769 1018 1575 2811 3199 4766
Size: 47 105 223 249 557 1236 388 1567
Cuts at: 4766 394
Size: 1522
Fragments arranged by size:
1567 1522 1236 557 388 249 223 105
47
ApaI G_GGCC'C
Cuts at: 2055 2567 2055
Size: 512 5382
ApaLI G'TGCA_C
Cuts at: 241 4664 241
Size: 4423 1471
ApoI r'AATT_y
Cuts at: 379 1744 2898 5247 5503 379
Size: 1365 1154 2349 256 770
Fragments arranged by size:
2349 1365 1154 770 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5894
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5894
AvaI C'yCGr_G
Cuts at: 355 923 2914 355
Size: 568 1991 3335
Fragments arranged by size:
3335 1991 568
BamHI G'GATC_C
Cuts at: 2919 2919
Size: 5894
BanI G'GyrC_C
Cuts at: 271 1340 1598 2910 3324 3572 3607 271
Size: 1069 258 1312 414 248 35 2558
Fragments arranged by size:
2558 1312 1069 414 258 248 35
BanII G_rGCy'C
Cuts at: 520 814 1185 1871 2055 2567 2582 2690 2846
Size: 294 371 686 184 512 15 108 156
Cuts at: 2846 2908 3938 520
Size: 62 1030 2476
Fragments arranged by size:
2476 1030 686 512 371 294 184 156
108 62 15
BbeI G_GCGC'C
Cuts at: 1602 3328 3576 1602
Size: 1726 248 3920
Fragments arranged by size:
3920 1726 248
BbsI GAAGACnn'nnnn_
Cuts at: 1377 1556 1377
Size: 179 5715
BbvCI CC'TCA_GC
Cuts at: 1850 1850
Size: 5894
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1782 1816 90
Size: 34 1658 34 4168
Fragments arranged by size:
4168 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1152 3792 4553 5894 1152
Size: 2640 761 1341 1152
Fragments arranged by size:
2640 1341 1152 761
BclI T'GATC_A
Cuts at: 3414 3414
Size: 5894
BfrBI ATG'CAT
Cuts at: 2615 2615
Size: 5894
BglI GCCn_nnn'nGGC
Cuts at: 2239 2239
Size: 5894
BglII A'GATC_T
Cuts at: 661 2321 3409 5446 661
Size: 1660 1088 2037 1109
Fragments arranged by size:
2037 1660 1109 1088
BlpI GC'TnA_GC
Cuts at: 593 1646 2576 3006 593
Size: 1053 930 430 3481
Fragments arranged by size:
3481 1053 930 430
Bme1580I G_kGCm'C
Cuts at: 245 641 719 2055 2367 2567 3519 3612 4668
Size: 396 78 1336 312 200 952 93 1056
Cuts at: 4668 245
Size: 1471
Fragments arranged by size:
1471 1336 1056 952 396 312 200 93
78
BmgBI CAC'GTC
Cuts at: 720 1844 720
Size: 1124 4770
BmrI ACTGGGnnnn_n'
Cuts at: 554 751 1677 1806 3106 3187 3522 5666 554
Size: 197 926 129 1300 81 335 2144 782
Fragments arranged by size:
2144 1300 926 782 335 197 129 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4261 4261
Size: 5894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 798 978 1158 1169 1656 1936 2833 2899 798
Size: 180 180 11 487 280 897 66 3793
Fragments arranged by size:
3793 897 487 280 180 180 66 11
Bpu10I CC'TnA_GC
Cuts at: 1350 1850 1350
Size: 500 5394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1770 3070 3949 4441 4739 686
Size: 1084 1300 879 492 298 1841
Fragments arranged by size:
1841 1300 1084 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5598 5598
Size: 5894
BsaAI yAC'GTr
Cuts at: 222 1696 3247 222
Size: 1474 1551 2869
Fragments arranged by size:
2869 1551 1474
BsaBI GATnn'nnATC
Cuts at: 6 2242 3408 5445 6
Size: 2236 1166 2037 455
Fragments arranged by size:
2236 2037 1166 455
BsaHI Gr'CG_yC
Cuts at: 1599 3325 3573 4275 5771 1599
Size: 1726 248 702 1496 1722
Fragments arranged by size:
1726 1722 1496 702 248
BsaWI w'CCGG_w
Cuts at: 191 1986 2150 3083 3604 4282 4556 4703 5114
Size: 1795 164 933 521 678 274 147 411
Cuts at: 5114 191
Size: 971
Fragments arranged by size:
1795 971 933 678 521 411 274 164
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1132 1162 1903 1933 1132
Size: 30 741 30 5093
Fragments arranged by size:
5093 741 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 981 1543 1546 2851 2854 981
Size: 562 3 1305 3 4021
Fragments arranged by size:
4021 1305 562 3 3
BseYI C'CCAG_C
Cuts at: 338 1306 2248 2559 3319 4077 4654 5150 338
Size: 968 942 311 760 758 577 496 1082
Fragments arranged by size:
1082 968 942 760 758 577 496 311
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 1819 2156 2368 755
Size: 1064 337 212 4281
Fragments arranged by size:
4281 1064 337 212
BsiEI CG_ry'CG
Cuts at: 104 3436 3482 4690 104
Size: 3332 46 1208 1308
Fragments arranged by size:
3332 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 245 814 1185 1351 1652 2582 2690 2908 3686
Size: 569 371 166 301 930 108 218 778
Cuts at: 3686 3876 4668 245
Size: 190 792 1471
Fragments arranged by size:
1471 930 792 778 569 371 301 218
190 166 108
BsmI GAATG_Cn'
Cuts at: 597 1455 2235 597
Size: 858 780 4256
Fragments arranged by size:
4256 858 780
BsmBI CGTCTCn'nnnn_
Cuts at: 726 726
Size: 5894
Bsp1286I G_dGCh'C
Cuts at: 245 520 641 719 814 1185 1351 1652 1871
Size: 275 121 78 95 371 166 301 219
Cuts at: 1871 2055 2367 2567 2582 2690 2846 2908 3519
Size: 184 312 200 15 108 156 62 611
Cuts at: 3519 3612 3686 3876 3938 4668 245
Size: 93 74 190 62 730 1471
Fragments arranged by size:
1471 730 611 371 312 301 275 219
200 190 184 166 156 121 108 95
93 78 74 62 62 15
BspEI T'CCGG_A
Cuts at: 2150 5114 2150
Size: 2964 2930
BspHI T'CATG_A
Cuts at: 5070 5070
Size: 5894
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1073 1631 2318 2944 3158 3460 3841 1073
Size: 558 687 626 214 302 381 3126
Fragments arranged by size:
3126 687 626 558 381 302 214
BsrBI CCG'CTC
Cuts at: 50 522 2772 4186 4240 5724 5889 50
Size: 472 2250 1414 54 1484 165 55
Fragments arranged by size:
2250 1484 1414 472 165 55 54
BsrDI GCAATG_nn'
Cuts at: 966 1166 1226 1248 3806 966
Size: 200 60 22 2558 3054
Fragments arranged by size:
3054 2558 200 60 22
BsrFI r'CCGG_y
Cuts at: 3892 4073 4282 3892
Size: 181 209 5504
Fragments arranged by size:
5504 209 181
BsrGI T'GTAC_A
Cuts at: 1316 1316
Size: 5894
BssSI C'ACGA_G
Cuts at: 244 511 2224 4165 4523 244
Size: 267 1713 1941 358 1615
Fragments arranged by size:
1941 1713 1615 358 267
BstAPI GCAn_nnn'nTGC
Cuts at: 315 2621 3271 315
Size: 2306 650 2938
Fragments arranged by size:
2938 2306 650
BstBI TT'CG_AA
Cuts at: 4255 4255
Size: 5894
BstEII G'GTnAC_C
Cuts at: 1665 1665
Size: 5894
BstYI r'GATC_y
Cuts at: 1 661 1221 1565 1586 2321 2919 3386 3409
Size: 660 560 344 21 735 598 467 23
Cuts at: 3409 3744 3990 5002 5088 5100 5446 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 735 660 598 560 467 449 346
344 335 246 86 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 1032 1032
Size: 5894
BtgI C'CryG_G
Cuts at: 1862 2111 2846 1862
Size: 249 735 4910
Fragments arranged by size:
4910 735 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3844 4025 4188 3844
Size: 181 163 5550
Fragments arranged by size:
5550 181 163
BtsI GCAGTG_nn'
Cuts at: 267 324 442 455 1296 1824 1972 3137 267
Size: 57 118 13 841 528 148 1165 3024
Fragments arranged by size:
3024 1165 841 528 148 118 57 13
ClaI AT'CG_AT
Cuts at: 485 485
Size: 5894
DraI TTT'AAA
Cuts at: 2893 2893
Size: 5894
DrdI GACnn_nn'nnGTC
Cuts at: 418 3600 4458 418
Size: 3182 858 1854
Fragments arranged by size:
3182 1854 858
EaeI y'GGCC_r
Cuts at: 425 902 1859 2114 2240 2456 2798 3479 3653
Size: 477 957 255 126 216 342 681 174
Cuts at: 3653 4044 4071 5416 425
Size: 391 27 1345 903
Fragments arranged by size:
1345 957 903 681 477 391 342 255
216 174 126 27
EagI C'GGCC_G
Cuts at: 3479 3479
Size: 5894
EarI CTCTTCn'nnn_
Cuts at: 1032 1538 2344 2705 3917 4127 1032
Size: 506 806 361 1212 210 2799
Fragments arranged by size:
2799 1212 806 506 361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1579 4102 4412 4558 5704 1579
Size: 2523 310 146 1146 1769
Fragments arranged by size:
2523 1769 1146 310 146
EcoICRI GAG'CTC
Cuts at: 812 1183 2580 2688 2906 812
Size: 371 1397 108 218 3800
Fragments arranged by size:
3800 1397 371 218 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 798 978 1158 1169 1443 1597 1656 1894 1936
Size: 180 180 11 274 154 59 238 42
Cuts at: 1936 2259 2833 2899 3719 4151 4898 5166 798
Size: 323 574 66 820 432 747 268 1526
Fragments arranged by size:
1526 820 747 574 432 323 274 268
238 180 180 154 66 59 42 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1441 1528 2503 1441
Size: 87 975 4832
Fragments arranged by size:
4832 975 87
EcoO109I rG'GnC_Cy
Cuts at: 611 889 1192 1264 2052 2307 2508 2563 2564
Size: 278 303 72 788 255 201 55 1
Cuts at: 2564 3033 611
Size: 469 3472
Fragments arranged by size:
3472 788 469 303 278 255 201 72
55 1
EcoRI G'AATT_C
Cuts at: 2898 2898
Size: 5894
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 683 2289 3913 683
Size: 1606 1624 2664
Fragments arranged by size:
2664 1624 1606
FspI TGC'GCA
Cuts at: 2263 3675 2263
Size: 1412 4482
HaeII r_GCGC'y
Cuts at: 1602 2857 3328 3576 4598 5577 1602
Size: 1255 471 248 1022 979 1919
Fragments arranged by size:
1919 1255 1022 979 471 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 356 388 646 678 704 736 1376 1408 1708
Size: 32 258 32 26 32 640 32 300
Cuts at: 1708 1740 2132 2164 2603 2635 3736 3766 3768
Size: 32 392 32 439 32 1101 30 2
Cuts at: 3768 3798 356
Size: 30 2452
Fragments arranged by size:
2452 1101 640 439 392 300 258 32
32 32 32 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 2933 2933
Size: 5894
HindIII A'AGCT_T
Cuts at: 1656 1656
Size: 5894
KasI G'GCGC_C
Cuts at: 1598 3324 3572 1598
Size: 1726 248 3920
Fragments arranged by size:
3920 1726 248
KpnI G_GTAC'C
Cuts at: 1344 2914 1344
Size: 1570 4324
MluI A'CGCG_T
Cuts at: 5327 5327
Size: 5894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4565 4749 4565
Size: 184 5710
MscI TGG'CCA
Cuts at: 904 1861 2116 2242 2458 2800 3655 904
Size: 957 255 126 216 342 855 3143
Fragments arranged by size:
3143 957 855 342 255 216 126
MslI CAynn'nnrTG
Cuts at: 238 427 2228 3883 4010 238
Size: 189 1801 1655 127 2122
Fragments arranged by size:
2122 1801 1655 189 127
MspA1I CmG'CkG
Cuts at: 941 1065 1427 2132 2418 2848 3005 3319 3679
Size: 124 362 705 286 430 157 314 360
Cuts at: 3679 4692 4937 941
Size: 1013 245 1898
Fragments arranged by size:
1898 1013 705 430 362 360 314 286
245 157 124
NaeI GCC'GGC
Cuts at: 4075 4075
Size: 5894
NarI GG'CG_CC
Cuts at: 1599 3325 3573 1599
Size: 1726 248 3920
Fragments arranged by size:
3920 1726 248
NcoI C'CATG_G
Cuts at: 1862 1862
Size: 5894
NgoMIV G'CCGG_C
Cuts at: 4073 4073
Size: 5894
NsiI A_TGCA'T
Cuts at: 2617 2617
Size: 5894
NspI r_CATG'y
Cuts at: 1365 2439 2619 2947 3447 4354 1365
Size: 1074 180 328 500 907 2905
Fragments arranged by size:
2905 1074 907 500 328 180
PciI A'CATG_T
Cuts at: 2435 4350 2435
Size: 1915 3979
PflMI CCAn_nnn'nTGG
Cuts at: 682 682
Size: 5894
PfoI T'CCnGG_A
Cuts at: 1185 1568 1928 2876 1185
Size: 383 360 948 4203
Fragments arranged by size:
4203 948 383 360
PmeI GTTT'AAAC
Cuts at: 2893 2893
Size: 5894
PmlI CAC'GTG
Cuts at: 222 1696 222
Size: 1474 4420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 960 992 1768 1800 5033 5065 960
Size: 32 776 32 3233 32 1789
Fragments arranged by size:
3233 1789 776 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 611 2307 2508 611
Size: 1696 201 3997
Fragments arranged by size:
3997 1696 201
PspOMI G'GGCC_C
Cuts at: 2051 2563 2051
Size: 512 5382
PstI C_TGCA'G
Cuts at: 308 320 437 2780 2941 3626 308
Size: 12 117 2343 161 685 2576
Fragments arranged by size:
2576 2343 685 161 117 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5894
PvuII CAG'CTG
Cuts at: 941 1065 1427 2418 3319 3679 941
Size: 124 362 991 901 360 3156
Fragments arranged by size:
3156 991 901 362 360 124
RsrII CG'GwC_CG
Cuts at: 4089 4089
Size: 5894
SacI G_AGCT'C
Cuts at: 814 1185 2582 2690 2908 814
Size: 371 1397 108 218 3800
Fragments arranged by size:
3800 1397 371 218 108
SacII CC_GC'GG
Cuts at: 2849 2849
Size: 5894
SalI G'TCGA_C
Cuts at: 2931 2931
Size: 5894
SapI GCTCTTCn'nnn_
Cuts at: 3917 4127 3917
Size: 210 5684
SbfI CC_TGCA'GG
Cuts at: 2941 2941
Size: 5894
ScaI AGT'ACT
Cuts at: 819 2206 2284 5411 819
Size: 1387 78 3127 1302
Fragments arranged by size:
3127 1387 1302 78
SfcI C'TryA_G
Cuts at: 33 304 316 433 2370 2776 2937 3622 4615
Size: 271 12 117 1937 406 161 685 993
Cuts at: 4615 4806 33
Size: 191 1121
Fragments arranged by size:
1937 1121 993 685 406 271 191 161
117 12
SfoI GGC'GCC
Cuts at: 1600 3326 3574 1600
Size: 1726 248 3920
Fragments arranged by size:
3920 1726 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5894
SmaI CCC'GGG
Cuts at: 2916 2916
Size: 5894
SmlI C'TyrA_G
Cuts at: 701 1785 3049 3964 4456 4718 701
Size: 1084 1264 915 492 262 1877
Fragments arranged by size:
1877 1264 1084 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5894
SphI G_CATG'C
Cuts at: 2619 2947 3447 2619
Size: 328 500 5066
Fragments arranged by size:
5066 500 328
StyI C'CwwG_G
Cuts at: 1862 2303 2459 3028 3382 1862
Size: 441 156 569 354 4374
Fragments arranged by size:
4374 569 441 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1320 3477 3610 4069 4278 1320
Size: 2157 133 459 209 2936
Fragments arranged by size:
2936 2157 459 209 133
TatI w'GTAC_w
Cuts at: 817 1316 2204 2282 2519 5409 817
Size: 499 888 78 237 2890 1302
Fragments arranged by size:
2890 1302 888 499 237 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1132 1164 1903 1935 5411 5443 1132
Size: 32 739 32 3476 32 1583
Fragments arranged by size:
3476 1583 739 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2485 3691 2485
Size: 1206 4688
XbaI T'CTAG_A
Cuts at: 2925 2925
Size: 5894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 912 1048 2466 2808 3389 912
Size: 136 1418 342 581 3417
Fragments arranged by size:
3417 1418 581 342 136
XmaI C'CCGG_G
Cuts at: 2914 2914
Size: 5894
XmnI GAAnn'nnTTC
Cuts at: 2437 2437
Size: 5894
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BmtI BsiWI BssHII BstXI BstZ17I CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PasI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI