Restriction map for
pF1KA0219
(Circular) (Six-base) MAPSORT of: pF1KA0219.seq Check: 5511 from: 1 to: 11126
pF1KA0219 11126 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 403 6683 8088 403
Size: 6280 1405 3441
Fragments arranged by size:
6280 3441 1405
AccI GT'mk_AC
Cuts at: 2750 4713 8164 2750
Size: 1963 3451 5712
Fragments arranged by size:
5712 3451 1963
Acc65I G'GTAC_C
Cuts at: 8142 8142
Size: 11126
AclI AA'CG_TT
Cuts at: 10966 10966
Size: 11126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 966 1273 1694 1951 2222 2557 2791 2911 3082
Size: 307 421 257 271 335 234 120 171
Cuts at: 3082 3112 3269 4036 4366 6322 6487 7150 7222
Size: 30 157 767 330 1956 165 663 72
Cuts at: 7222 7502 7909 8083 8951 9383 9736 10398 966
Size: 280 407 174 868 432 353 662 1694
Fragments arranged by size:
1956 1694 868 767 663 662 432 421
407 353 335 330 307 280 271 257
234 174 171 165 157 120 72 30
AflIII A'CryG_T
Cuts at: 5030 5917 7526 8478 10278 10559 5030
Size: 887 1609 952 1800 281 5597
Fragments arranged by size:
5597 1800 1609 952 887 281
AleI CACnn'nnGTG
Cuts at: 1089 2863 3092 6382 1089
Size: 1774 229 3290 5833
Fragments arranged by size:
5833 3290 1774 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 331 1107 1151 2381 2429 6635 331
Size: 776 44 1230 48 4206 4822
Fragments arranged by size:
4822 4206 1230 776 48 44
AlwNI CAG_nnn'CTG
Cuts at: 549 1251 1494 1612 1646 2658 3016 3279 3454
Size: 702 243 118 34 1012 358 263 175
Cuts at: 3454 4260 4263 4902 5254 5677 6228 6409 7329
Size: 806 3 639 352 423 551 181 920
Cuts at: 7329 8431 9869 549
Size: 1102 1438 1806
Fragments arranged by size:
1806 1438 1102 1012 920 806 702 639
551 423 358 352 263 243 181 175
118 34 3
ApaI G_GGCC'C
Cuts at: 5438 5438
Size: 11126
ApaLI G'TGCA_C
Cuts at: 9964 9964
Size: 11126
ApoI r'AATT_y
Cuts at: 877 1693 8130 10479 10735 877
Size: 816 6437 2349 256 1268
Fragments arranged by size:
6437 2349 1268 816 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 11126
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11126
AvaI C'yCGr_G
Cuts at: 637 3178 5992 6380 6690 7087 8146 637
Size: 2541 2814 388 310 397 1059 3617
Fragments arranged by size:
3617 2814 2541 1059 397 388 310
AvrII C'CTAG_G
Cuts at: 7455 7455
Size: 11126
BamHI G'GATC_C
Cuts at: 4866 7670 8151 4866
Size: 2804 481 7841
Fragments arranged by size:
7841 2804 481
BanI G'GyrC_C
Cuts at: 2219 2921 6551 7451 8142 8556 8804 8839 2219
Size: 702 3630 900 691 414 248 35 4506
Fragments arranged by size:
4506 3630 900 702 691 414 248 35
BanII G_rGCy'C
Cuts at: 257 683 1812 2075 2293 3262 4081 4422 5236
Size: 426 1129 263 218 969 819 341 814
Cuts at: 5236 5438 5894 8140 9170 257
Size: 202 456 2246 1030 2213
Fragments arranged by size:
2246 2213 1129 1030 969 819 814 456
426 341 263 218 202
BbeI G_GCGC'C
Cuts at: 8560 8808 8560
Size: 248 10878
BbsI GAAGACnn'nnnn_
Cuts at: 383 1939 3042 3226 4885 383
Size: 1556 1103 184 1659 6624
Fragments arranged by size:
6624 1659 1556 1103 184
BbvCI CC'TCA_GC
Cuts at: 341 1625 3154 5270 7443 341
Size: 1284 1529 2116 2173 4024
Fragments arranged by size:
4024 2173 2116 1529 1284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1558 1592 1852 1886 1558
Size: 34 260 34 10798
Fragments arranged by size:
10798 260 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5461 9024 10080 11126 5461
Size: 3563 1056 1046 5461
Fragments arranged by size:
5461 3563 1056 1046
BclI T'GATC_A
Cuts at: 2120 8646 2120
Size: 6526 4600
BglI GCCn_nnn'nGGC
Cuts at: 112 319 451 2841 4313 5152 8080 112
Size: 207 132 2390 1472 839 2928 3158
Fragments arranged by size:
3158 2928 2390 1472 839 207 132
BglII A'GATC_T
Cuts at: 228 5958 8641 10678 228
Size: 5730 2683 2037 676
Fragments arranged by size:
5730 2683 2037 676
BlpI GC'TnA_GC
Cuts at: 1268 1859 7402 8238 1268
Size: 591 5543 836 4156
Fragments arranged by size:
5543 4156 836 591
Bme1580I G_kGCm'C
Cuts at: 849 1373 2454 2860 4730 5438 6131 6353 6554
Size: 524 1081 406 1870 708 693 222 201
Cuts at: 6554 8751 8844 9968 849
Size: 2197 93 1124 2007
Fragments arranged by size:
2197 2007 1870 1124 1081 708 693 524
406 222 201 93
BmgBI CAC'GTC
Cuts at: 750 1845 3363 3609 7527 750
Size: 1095 1518 246 3918 4349
Fragments arranged by size:
4349 3918 1518 1095 246
BmrI ACTGGGnnnn_n'
Cuts at: 416 2724 3742 3837 5666 5703 8338 8419 8754
Size: 2308 1018 95 1829 37 2635 81 335
Cuts at: 8754 10898 416
Size: 2144 644
Fragments arranged by size:
2635 2308 2144 1829 1018 644 335 95
81 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 5250 9493 211
Size: 5039 4243 1844
Fragments arranged by size:
5039 4243 1844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 2009 2551 2692 3773 4250 4285 4645 4826
Size: 1429 542 141 1081 477 35 360 181
Cuts at: 4826 4857 6181 6250 6520 6598 6702 6754 580
Size: 31 1324 69 270 78 104 52 4952
Fragments arranged by size:
4952 1429 1324 1081 542 477 360 270
181 141 104 78 69 52 35 31
Bpu10I CC'TnA_GC
Cuts at: 341 1625 1828 3154 4694 4792 5247 5270 6938
Size: 1284 203 1326 1540 98 455 23 1668
Cuts at: 6938 7112 7443 7648 341
Size: 174 331 205 3819
Fragments arranged by size:
3819 1668 1540 1326 1284 455 331 205
203 174 98 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 2830 4596 6031 7951 8302 9181 9895 10193
Size: 771 1766 1435 1920 351 879 714 298
Cuts at: 10193 2059
Size: 2992
Fragments arranged by size:
2992 1920 1766 1435 879 771 714 351
298
BsaI GGTCTCn'nnnn_
Cuts at: 6032 6693 10830 6032
Size: 661 4137 6328
Fragments arranged by size:
6328 4137 661
BsaAI yAC'GTr
Cuts at: 1224 2019 2874 4485 5918 8479 1224
Size: 795 855 1611 1433 2561 3871
Fragments arranged by size:
3871 2561 1611 1433 855 795
BsaBI GATnn'nnATC
Cuts at: 6 2191 6562 7042 8640 10677 6
Size: 2185 4371 480 1598 2037 455
Fragments arranged by size:
4371 2185 2037 1598 480 455
BsaHI Gr'CG_yC
Cuts at: 5083 5815 6998 8557 8805 9507 11003 5083
Size: 732 1183 1559 248 702 1496 5206
Fragments arranged by size:
5206 1559 1496 1183 732 702 248
BsaWI w'CCGG_w
Cuts at: 2059 2639 3530 3923 6554 7376 8315 8836 9514
Size: 580 891 393 2631 822 939 521 678
Cuts at: 9514 9925 10072 10346 2059
Size: 411 147 274 2839
Fragments arranged by size:
2839 2631 939 891 822 678 580 521
411 393 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1200 1230 2032 2062 2257 2287 3094 3124 3574
Size: 30 802 30 195 30 807 30 450
Cuts at: 3574 3604 3823 3853 4621 4651 5020 5050 5948
Size: 30 219 30 768 30 369 30 898
Cuts at: 5948 5978 1200
Size: 30 6348
Fragments arranged by size:
6348 898 807 802 768 450 369 219
195 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 936 1939 2404 2557 2587 3112 3115 3118 3121
Size: 1003 465 153 30 525 3 3 3
Cuts at: 3121 3367 3535 3538 3776 4021 4642 6178 6286
Size: 246 168 3 238 245 621 1536 108
Cuts at: 6286 6751 6964 7431 7501 7954 936
Size: 465 213 467 70 453 4108
Fragments arranged by size:
4108 1536 1003 621 525 467 465 465
453 246 245 238 213 168 153 108
70 30 3 3 3 3
BseYI C'CCAG_C
Cuts at: 347 567 705 2416 2453 2615 3149 4163 4452
Size: 220 138 1711 37 162 534 1014 289
Cuts at: 4452 4651 5074 5561 5795 5898 6447 6743 7145
Size: 199 423 487 234 103 549 296 402
Cuts at: 7145 7410 8551 9309 9974 10382 347
Size: 265 1141 758 665 408 1091
Fragments arranged by size:
1711 1141 1091 1014 758 665 549 534
487 423 408 402 296 289 265 234
220 199 162 138 103 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 711 739 1113 2173 2351 2377 2599 2953
Size: 149 28 374 1060 178 26 222 354
Cuts at: 2953 4428 5240 5942 6796 562
Size: 1475 812 702 854 4892
Fragments arranged by size:
4892 1475 1060 854 812 702 374 354
222 178 149 28 26
BsiEI CG_ry'CG
Cuts at: 104 3742 8668 8714 9944 104
Size: 3638 4926 46 1230 1286
Fragments arranged by size:
4926 3638 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1826 3416 4584 4604 4692 5236 8140 8918 9108
Size: 1590 1168 20 88 544 2904 778 190
Cuts at: 9108 9968 1826
Size: 860 2984
Fragments arranged by size:
2984 2904 1590 1168 860 778 544 190
88 20
BsmI GAATG_Cn'
Cuts at: 2286 6204 2286
Size: 3918 7208
BsmBI CGTCTCn'nnnn_
Cuts at: 7395 7395
Size: 11126
Bsp1286I G_dGCh'C
Cuts at: 257 683 849 1373 1812 1826 2075 2293 2454
Size: 426 166 524 439 14 249 218 161
Cuts at: 2454 2860 3262 3416 4081 4422 4584 4604 4692
Size: 406 402 154 665 341 162 20 88
Cuts at: 4692 4730 5236 5438 5894 6131 6353 6554 8140
Size: 38 506 202 456 237 222 201 1586
Cuts at: 8140 8751 8844 8918 9108 9170 9968 257
Size: 611 93 74 190 62 798 1415
Fragments arranged by size:
1586 1415 798 665 611 524 506 456
439 426 406 402 341 249 237 222
218 202 201 190 166 162 161 154
93 88 74 62 38 20 14
BspEI T'CCGG_A
Cuts at: 2639 3530 3923 7376 2639
Size: 891 393 3453 6389
Fragments arranged by size:
6389 3453 891 393
BspHI T'CATG_A
Cuts at: 1302 3618 6077 7712 9558 1302
Size: 2316 2459 1635 1846 2870
Fragments arranged by size:
2870 2459 2316 1846 1635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 114 403 475 1454 1665 2625 3581 4158 5078
Size: 289 72 979 211 960 956 577 920
Cuts at: 5078 5402 6683 8088 8176 8390 8692 9073 114
Size: 324 1281 1405 88 214 302 381 2167
Fragments arranged by size:
2167 1405 1281 979 960 956 920 577
381 324 302 289 214 211 88 72
BsrBI CCG'CTC
Cuts at: 50 5748 5786 6650 7688 9418 9472 10956 11121
Size: 5698 38 864 1038 1730 54 1484 165
Cuts at: 11121 50
Size: 55
Fragments arranged by size:
5698 1730 1484 1038 864 165 55 54
38
BsrDI GCAATG_nn'
Cuts at: 4682 4835 5348 7676 9038 4682
Size: 153 513 2328 1362 6770
Fragments arranged by size:
6770 2328 1362 513 153
BsrFI r'CCGG_y
Cuts at: 3737 4406 5281 7612 7749 9124 9305 3737
Size: 669 875 2331 137 1375 181 5558
Fragments arranged by size:
5558 2331 1375 875 669 181 137
BsrGI T'GTAC_A
Cuts at: 5774 5774
Size: 11126
BssHII G'CGCG_C
Cuts at: 5502 5502
Size: 11126
BssSI C'ACGA_G
Cuts at: 729 4204 6124 9397 10105 729
Size: 3475 1920 3273 708 1750
Fragments arranged by size:
3475 3273 1920 1750 708
BstAPI GCAn_nnn'nTGC
Cuts at: 549 2179 4260 4902 7515 8503 549
Size: 1630 2081 642 2613 988 3172
Fragments arranged by size:
3172 2613 2081 1630 988 642
BstBI TT'CG_AA
Cuts at: 9487 9487
Size: 11126
BstEII G'GTnAC_C
Cuts at: 1021 1021
Size: 11126
BstXI CCAn_nnnn'nTGG
Cuts at: 2223 2994 3797 4095 4777 5023 5343 6726 8013
Size: 771 803 298 682 246 320 1383 1287
Cuts at: 8013 2223
Size: 5336
Fragments arranged by size:
5336 1383 1287 803 771 682 320 298
246
BstYI r'GATC_y
Cuts at: 1 228 885 3131 3527 4826 4866 5628 5870
Size: 227 657 2246 396 1299 40 762 242
Cuts at: 5870 5958 6570 7670 8009 8151 8618 8641 8976
Size: 88 612 1100 339 142 467 23 335
Cuts at: 8976 9222 9528 9540 9626 10678 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2246 1299 1100 1052 762 657 612 467
449 396 339 335 306 246 242 227
142 88 86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 5120 6022 6596 6966 7003 7330 5120
Size: 902 574 370 37 327 8916
Fragments arranged by size:
8916 902 574 370 327 37
BtgI C'CryG_G
Cuts at: 107 590 995 1109 2026 2285 4991 5156 5200
Size: 483 405 114 917 259 2706 165 44
Cuts at: 5200 6131 7013 7289 7577 7658 7925 8090 107
Size: 931 882 276 288 81 267 165 3143
Fragments arranged by size:
3143 2706 931 917 882 483 405 288
276 267 259 165 165 114 81 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4125 5084 5529 6053 9076 9257 9420 4125
Size: 959 445 524 3023 181 163 5831
Fragments arranged by size:
5831 3023 959 524 445 181 163
BtsI GCAGTG_nn'
Cuts at: 251 359 438 550 1069 1101 1456 2345 2368
Size: 108 79 112 519 32 355 889 23
Cuts at: 2368 3324 3671 5729 7516 7632 7659 7849 8369
Size: 956 347 2058 1787 116 27 190 520
Cuts at: 8369 251
Size: 3008
Fragments arranged by size:
3008 2058 1787 956 889 520 519 355
347 190 116 112 108 79 32 27
23
ClaI AT'CG_AT
Cuts at: 285 2195 285
Size: 1910 9216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5868 5903 5868
Size: 35 11091
DraI TTT'AAA
Cuts at: 8125 8125
Size: 11126
DraIII CAC_nnn'GTG
Cuts at: 591 1227 1956 3305 4590 591
Size: 636 729 1349 1285 7127
Fragments arranged by size:
7127 1349 1285 729 636
DrdI GACnn_nn'nnGTC
Cuts at: 1019 1241 4759 7303 8832 10176 1019
Size: 222 3518 2544 1529 1344 1969
Fragments arranged by size:
3518 2544 1969 1529 1344 222
EaeI y'GGCC_r
Cuts at: 1106 2167 2705 3221 3739 4085 4832 5279 5909
Size: 1061 538 516 518 346 747 447 630
Cuts at: 5909 6216 6506 7751 8021 8072 8711 8885 9276
Size: 307 290 1245 270 51 639 174 391
Cuts at: 9276 9303 10648 1106
Size: 27 1345 1584
Fragments arranged by size:
1584 1345 1245 1061 747 639 630 538
518 516 447 391 346 307 290 270
174 51 27
EagI C'GGCC_G
Cuts at: 3739 8711 3739
Size: 4972 6154
EarI CTCTTCn'nnn_
Cuts at: 1107 2924 2944 6028 6712 6800 7264 7478 7901
Size: 1817 20 3084 684 88 464 214 423
Cuts at: 7901 7979 9149 9359 1107
Size: 78 1170 210 2874
Fragments arranged by size:
3084 2874 1817 1170 684 464 423 214
210 88 78 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 3706 4393 4656 5766 6691 6970 7537 7575
Size: 3576 687 263 1110 925 279 567 38
Cuts at: 7575 7678 7751 9334 10076 10222 10936 130
Size: 103 73 1583 742 146 714 320
Fragments arranged by size:
3576 1583 1110 925 742 714 687 567
320 279 263 146 103 73 38
EcoICRI GAG'CTC
Cuts at: 5234 8138 5234
Size: 2904 8222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 966 1273 1694 1951 2009 2222 2551 2557
Size: 386 307 421 257 58 213 329 6
Cuts at: 2557 2692 2791 2911 3082 3112 3269 3773 4036
Size: 135 99 120 171 30 157 504 263
Cuts at: 4036 4250 4285 4366 4645 4826 4857 6181 6250
Size: 214 35 81 279 181 31 1324 69
Cuts at: 6250 6322 6487 6520 6598 6702 6754 7150 7222
Size: 72 165 33 78 104 52 396 72
Cuts at: 7222 7502 7909 8083 8951 9383 9736 10398 580
Size: 280 407 174 868 432 353 662 1308
Fragments arranged by size:
1324 1308 868 662 504 432 421 407
396 386 353 329 307 280 279 263
257 214 213 181 174 171 165 157
135 120 104 99 81 78 72 72
69 58 52 35 33 31 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 306 918 1291 2038 4321 4627 306
Size: 612 373 747 2283 306 6805
Fragments arranged by size:
6805 2283 747 612 373 306
EcoO109I rG'GnC_Cy
Cuts at: 311 1248 1310 1491 2031 2319 3158 4326 5145
Size: 937 62 181 540 288 839 1168 819
Cuts at: 5145 5434 6482 7007 7296 7326 7845 7934 8057
Size: 289 1048 525 289 30 519 89 123
Cuts at: 8057 8265 311
Size: 208 3172
Fragments arranged by size:
3172 1168 1048 937 839 819 540 525
519 289 289 288 208 181 123 89
62 30
EcoRI G'AATT_C
Cuts at: 8130 8130
Size: 11126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2823 3891 4416 5119 6161 7507 9145 2823
Size: 1068 525 703 1042 1346 1638 4804
Fragments arranged by size:
4804 1638 1346 1068 1042 703 525
FspI TGC'GCA
Cuts at: 481 8907 481
Size: 8426 2700
FspAI rTGC'GCAy
Cuts at: 481 481
Size: 11126
HaeII r_GCGC'y
Cuts at: 773 2683 4792 5255 8560 8808 10038 10809 773
Size: 1910 2109 463 3305 248 1230 771 1090
Fragments arranged by size:
3305 2109 1910 1230 1090 771 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 286 299 318 1200 1232 3574 3606 4065
Size: 19 13 19 882 32 2342 32 459
Cuts at: 4065 4097 4223 4255 4618 4650 4924 4956 6262
Size: 32 126 32 363 32 274 32 1306
Cuts at: 6262 6294 6857 6889 7709 7741 8968 8998 9000
Size: 32 563 32 820 32 1227 30 2
Cuts at: 9000 9030 267
Size: 30 2363
Fragments arranged by size:
2363 2342 1306 1227 882 820 563 459
363 274 126 32 32 32 32 32
32 32 32 32 30 30 19 19
13 2
HincII GTy'rAC
Cuts at: 2751 3984 6375 7959 8049 8165 2751
Size: 1233 2391 1584 90 116 5712
Fragments arranged by size:
5712 2391 1584 1233 116 90
HindIII A'AGCT_T
Cuts at: 1400 2498 4732 6475 1400
Size: 1098 2234 1743 6051
Fragments arranged by size:
6051 2234 1743 1098
HpaI GTT'AAC
Cuts at: 8049 8049
Size: 11126
KasI G'GCGC_C
Cuts at: 8556 8804 8556
Size: 248 10878
KpnI G_GTAC'C
Cuts at: 8146 8146
Size: 11126
MfeI C'AATT_G
Cuts at: 6510 7952 6510
Size: 1442 9684
MluI A'CGCG_T
Cuts at: 10559 10559
Size: 11126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 815 1058 3917 5282 5738 7208 9885 10069 815
Size: 243 2859 1365 456 1470 2677 184 1872
Fragments arranged by size:
2859 2677 1872 1470 1365 456 243 184
MscI TGG'CCA
Cuts at: 2169 2707 3223 4087 4834 5911 6508 8023 8074
Size: 538 516 864 747 1077 597 1515 51
Cuts at: 8074 8887 2169
Size: 813 4408
Fragments arranged by size:
4408 1515 1077 864 813 747 597 538
516 51
MslI CAynn'nnrTG
Cuts at: 802 1089 2863 3092 3917 5380 6382 7246 7681
Size: 287 1774 229 825 1463 1002 864 435
Cuts at: 7681 9115 9242 802
Size: 1434 127 2686
Fragments arranged by size:
2686 1774 1463 1434 1002 864 825 435
287 229 127
MspA1I CmG'CkG
Cuts at: 2583 3153 3681 4190 4260 4701 5514 5799 6201
Size: 570 528 509 70 441 813 285 402
Cuts at: 6201 6261 6451 6498 7064 7149 8237 8551 8911
Size: 60 190 47 566 85 1088 314 360
Cuts at: 8911 9695 9940 2583
Size: 784 245 3769
Fragments arranged by size:
3769 1088 813 784 570 566 528 509
441 402 360 314 285 245 190 85
70 60 47
NaeI GCC'GGC
Cuts at: 4408 7614 7751 9307 4408
Size: 3206 137 1556 6227
Fragments arranged by size:
6227 3206 1556 137
NarI GG'CG_CC
Cuts at: 8557 8805 8557
Size: 248 10878
NcoI C'CATG_G
Cuts at: 107 995 1109 2285 5156 7289 107
Size: 888 114 1176 2871 2133 3944
Fragments arranged by size:
3944 2871 2133 1176 888 114
NgoMIV G'CCGG_C
Cuts at: 4406 7612 7749 9305 4406
Size: 3206 137 1556 6227
Fragments arranged by size:
6227 3206 1556 137
NspI r_CATG'y
Cuts at: 705 1441 1458 3936 5034 5827 6859 7410 8179
Size: 736 17 2478 1098 793 1032 551 769
Cuts at: 8179 8679 10282 705
Size: 500 1603 1549
Fragments arranged by size:
2478 1603 1549 1098 1032 793 769 736
551 500 17
PasI CC'CwG_GG
Cuts at: 5736 5736
Size: 11126
PciI A'CATG_T
Cuts at: 5030 10278 5030
Size: 5248 5878
PflMI CCAn_nnn'nTGG
Cuts at: 336 1524 3666 3849 5802 6228 336
Size: 1188 2142 183 1953 426 5234
Fragments arranged by size:
5234 2142 1953 1188 426 183
PfoI T'CCnGG_A
Cuts at: 2906 4172 4850 4873 4979 8012 2906
Size: 1266 678 23 106 3033 6020
Fragments arranged by size:
6020 3033 1266 678 106 23
PmeI GTTT'AAAC
Cuts at: 8125 8125
Size: 11126
PmlI CAC'GTG
Cuts at: 1224 2874 4485 5918 1224
Size: 1650 1611 1433 6432
Fragments arranged by size:
6432 1650 1611 1433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3970 4002 9572 9604 3970
Size: 32 5570 32 5492
Fragments arranged by size:
5570 5492 32 32
PpuMI rG'GwC_Cy
Cuts at: 1248 1310 2031 2319 6482 7296 7845 8057 1248
Size: 62 721 288 4163 814 549 212 4317
Fragments arranged by size:
4317 4163 814 721 549 288 212 62
PspOMI G'GGCC_C
Cuts at: 5434 5434
Size: 11126
PstI C_TGCA'G
Cuts at: 389 1451 2039 2612 2654 3578 4545 7154 7178
Size: 1062 588 573 42 924 967 2609 24
Cuts at: 7178 7325 7787 8173 8858 389
Size: 147 462 386 685 2657
Fragments arranged by size:
2657 2609 1062 967 924 685 588 573
462 386 147 42 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11126
PvuII CAG'CTG
Cuts at: 2583 3153 4190 4260 4701 5799 6201 6261 6498
Size: 570 1037 70 441 1098 402 60 237
Cuts at: 6498 7064 7149 8551 8911 2583
Size: 566 85 1402 360 4798
Fragments arranged by size:
4798 1402 1098 1037 570 566 441 402
360 237 85 70 60
RsrII CG'GwC_CG
Cuts at: 2313 5129 9321 2313
Size: 2816 4192 4118
Fragments arranged by size:
4192 4118 2816
SacI G_AGCT'C
Cuts at: 5236 8140 5236
Size: 2904 8222
SalI G'TCGA_C
Cuts at: 2749 8163 2749
Size: 5414 5712
SanDI GG'GwC_CC
Cuts at: 6482 6482
Size: 11126
SapI GCTCTTCn'nnn_
Cuts at: 2924 6028 6800 7901 9149 9359 2924
Size: 3104 772 1101 1248 210 4691
Fragments arranged by size:
4691 3104 1248 1101 772 210
SbfI CC_TGCA'GG
Cuts at: 2039 7178 8173 2039
Size: 5139 995 4992
Fragments arranged by size:
5139 4992 995
ScaI AGT'ACT
Cuts at: 655 10643 655
Size: 9988 1138
SfcI C'TryA_G
Cuts at: 33 385 1447 2035 2608 2650 3574 4541 5330
Size: 352 1062 588 573 42 924 967 789
Cuts at: 5330 7150 7174 7321 7504 7783 7938 8169 8854
Size: 1820 24 147 183 279 155 231 685
Cuts at: 8854 9822 10013 33
Size: 968 191 1146
Fragments arranged by size:
1820 1146 1062 968 967 924 789 685
588 573 352 279 231 191 183 155
147 42 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 4313 5152 8080 4313
Size: 839 2928 7359
Fragments arranged by size:
7359 2928 839
SfoI GGC'GCC
Cuts at: 8558 8806 8558
Size: 248 10878
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11126
SgrAI Cr'CCGG_yG
Cuts at: 4406 4406
Size: 11126
SmaI CCC'GGG
Cuts at: 639 3180 6382 8148 639
Size: 2541 3202 1766 3617
Fragments arranged by size:
3617 3202 2541 1766
SmlI C'TyrA_G
Cuts at: 2074 2845 4575 6010 7087 7966 8281 9196 9910
Size: 771 1730 1435 1077 879 315 915 714
Cuts at: 9910 10172 2074
Size: 262 3028
Fragments arranged by size:
3028 1730 1435 1077 915 879 771 714
315 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11126
SphI G_CATG'C
Cuts at: 705 1441 7410 8179 8679 705
Size: 736 5969 769 500 3152
Fragments arranged by size:
5969 3152 769 736 500
StuI AGG'CCT
Cuts at: 2014 5576 6020 2014
Size: 3562 444 7120
Fragments arranged by size:
7120 3562 444
StyI C'CwwG_G
Cuts at: 107 995 1109 1313 2285 2816 4556 5148 5156
Size: 888 114 204 972 531 1740 592 8
Cuts at: 5156 5622 6290 7289 7455 7910 8260 8614 107
Size: 466 668 999 166 455 350 354 2619
Fragments arranged by size:
2619 1740 999 972 888 668 592 531
466 455 354 350 204 166 114 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 559 2150 2989 3234 3489 3923 3929 4765 5809
Size: 1591 839 245 255 434 6 836 1044
Cuts at: 5809 8709 8842 9301 9510 559
Size: 2900 133 459 209 2175
Fragments arranged by size:
2900 2175 1591 1044 839 836 459 434
255 245 209 133 6
TatI w'GTAC_w
Cuts at: 653 1726 2732 5033 5774 10641 653
Size: 1073 1006 2301 741 4867 1138
Fragments arranged by size:
4867 2301 1138 1073 1006 741
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 529 561 1015 1047 1539 1571 1708 1740 2032
Size: 32 454 32 492 32 137 32 292
Cuts at: 2032 2064 2122 2154 2241 2273 3094 3126 3793
Size: 32 58 32 87 32 821 32 667
Cuts at: 3793 3825 4621 4653 5345 5377 5948 5980 10643
Size: 32 796 32 692 32 571 32 4663
Cuts at: 10643 10675 529
Size: 32 980
Fragments arranged by size:
4663 980 821 796 692 667 571 492
454 292 137 87 58 32 32 32
32 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 751 2317 8923 751
Size: 1566 6606 2954
Fragments arranged by size:
6606 2954 1566
XbaI T'CTAG_A
Cuts at: 8157 8157
Size: 11126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 694 2283 3813 5365 8097 8621 333
Size: 361 1589 1530 1552 2732 524 2838
Fragments arranged by size:
2838 2732 1589 1552 1530 524 361
XhoI C'TCGA_G
Cuts at: 7087 7087
Size: 11126
XmaI C'CCGG_G
Cuts at: 637 3178 6380 8146 637
Size: 2541 3202 1766 3617
Fragments arranged by size:
3617 3202 2541 1766
XmnI GAAnn'nnTTC
Cuts at: 2806 4399 7135 2806
Size: 1593 2736 6797
Fragments arranged by size:
6797 2736 1593
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI BaeI
BfrBI BmtI BsiWI BstZ17I EcoRV FseI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SexAI SnaBI SrfI SspI SwaI ZraI