Restriction map for
pF1KA0203
(Circular) (Six-base) MAPSORT of: pF1KA0203.seq Check: 6948 from: 1 to: 7886
pF1KA0203 7886 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 369 369
Size: 7886
AccI GT'mk_AC
Cuts at: 2430 2541 3534 4924 2430
Size: 111 993 1390 5392
Fragments arranged by size:
5392 1390 993 111
Acc65I G'GTAC_C
Cuts at: 4902 4902
Size: 7886
AclI AA'CG_TT
Cuts at: 7726 7726
Size: 7886
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 2520 2783 2980 3031 3076 3976 4244 4487
Size: 1037 263 197 51 45 900 268 243
Cuts at: 4487 4550 4721 5711 6143 6496 7158 1483
Size: 63 171 990 432 353 662 2211
Fragments arranged by size:
2211 1037 990 900 662 432 353 268
263 243 197 171 63 51 45
AflII C'TTAA_G
Cuts at: 190 190
Size: 7886
AflIII A'CryG_T
Cuts at: 897 1452 5238 7038 7319 897
Size: 555 3786 1800 281 1464
Fragments arranged by size:
3786 1800 1464 555 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1961 1961
Size: 7886
AlwNI CAG_nnn'CTG
Cuts at: 240 922 1860 3319 5191 6629 240
Size: 682 938 1459 1872 1438 1497
Fragments arranged by size:
1872 1497 1459 1438 938 682
ApaLI G'TGCA_C
Cuts at: 4346 6724 4346
Size: 2378 5508
ApoI r'AATT_y
Cuts at: 591 2337 2610 2842 3332 3402 3850 4890 7239
Size: 1746 273 232 490 70 448 1040 2349
Cuts at: 7239 7495 591
Size: 256 982
Fragments arranged by size:
2349 1746 1040 982 490 448 273 256
232 70
AseI AT'TA_AT
Cuts at: 20 3182 20
Size: 3162 4724
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7886
AvaI C'yCGr_G
Cuts at: 4906 4906
Size: 7886
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2435 2468 2435
Size: 33 7853
BamHI G'GATC_C
Cuts at: 2139 4911 2139
Size: 2772 5114
BanI G'GyrC_C
Cuts at: 4902 5316 5564 5599 4902
Size: 414 248 35 7189
Fragments arranged by size:
7189 414 248 35
BanII G_rGCy'C
Cuts at: 3357 4236 4900 5930 3357
Size: 879 664 1030 5313
Fragments arranged by size:
5313 1030 879 664
BbeI G_GCGC'C
Cuts at: 5320 5568 5320
Size: 248 7638
BbsI GAAGACnn'nnnn_
Cuts at: 662 768 794 2564 2993 4556 4758 662
Size: 106 26 1770 429 1563 202 3790
Fragments arranged by size:
3790 1770 1563 429 202 106 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4048 4082 4048
Size: 34 7852
BciVI GTATCCnnnnn_n'
Cuts at: 300 2436 5784 6840 7886 300
Size: 2136 3348 1056 1046 300
Fragments arranged by size:
3348 2136 1056 1046 300
BclI T'GATC_A
Cuts at: 1494 5406 1494
Size: 3912 3974
BfrBI ATG'CAT
Cuts at: 263 1324 263
Size: 1061 6825
BglI GCCn_nnn'nGGC
Cuts at: 4804 4804
Size: 7886
BglII A'GATC_T
Cuts at: 2007 5401 7438 2007
Size: 3394 2037 2455
Fragments arranged by size:
3394 2455 2037
BlpI GC'TnA_GC
Cuts at: 1599 3471 4998 1599
Size: 1872 1527 4487
Fragments arranged by size:
4487 1872 1527
Bme1580I G_kGCm'C
Cuts at: 4350 5511 5604 6728 4350
Size: 1161 93 1124 5508
Fragments arranged by size:
5508 1161 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 5098 5179 5514 7658 5098
Size: 81 335 2144 5326
Fragments arranged by size:
5326 2144 335 81
BmtI G_CTAG'C
Cuts at: 1241 1241
Size: 7886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 6253 2135
Size: 4118 3768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 2165 598
Size: 1567 6319
Bpu10I CC'TnA_GC
Cuts at: 1277 1277
Size: 7886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2833 3235 3763 3928 4264 5062 5941 6655 6953
Size: 402 528 165 336 798 879 714 298
Cuts at: 6953 2833
Size: 3766
Fragments arranged by size:
3766 879 798 714 528 402 336 298
165
BsaI GGTCTCn'nnnn_
Cuts at: 3361 7590 3361
Size: 4229 3657
BsaAI yAC'GTr
Cuts at: 5239 5239
Size: 7886
BsaBI GATnn'nnATC
Cuts at: 6 311 536 1004 5400 7437 6
Size: 305 225 468 4396 2037 455
Fragments arranged by size:
4396 2037 468 455 305 225
BsaHI Gr'CG_yC
Cuts at: 5317 5565 6267 7763 5317
Size: 248 702 1496 5440
Fragments arranged by size:
5440 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5075 5596 6274 6685 6832 7106 5075
Size: 521 678 411 147 274 5855
Fragments arranged by size:
5855 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2789 2819 4340 4370 4610 4640 2789
Size: 30 1521 30 240 30 6035
Fragments arranged by size:
6035 1521 240 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 269 2108 2830 3965 269
Size: 1839 722 1135 4190
Fragments arranged by size:
4190 1839 1135 722
BseYI C'CCAG_C
Cuts at: 298 554 3356 5311 6069 6734 7142 298
Size: 256 2802 1955 758 665 408 1042
Fragments arranged by size:
2802 1955 1042 758 665 408 256
BsiEI CG_ry'CG
Cuts at: 104 5428 5474 6704 104
Size: 5324 46 1230 1286
Fragments arranged by size:
5324 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 833 3675 4236 4350 4900 5678 5868 6728 833
Size: 2842 561 114 550 778 190 860 1991
Fragments arranged by size:
2842 1991 860 778 561 550 190 114
BsmI GAATG_Cn'
Cuts at: 265 1150 2441 4581 265
Size: 885 1291 2140 3570
Fragments arranged by size:
3570 2140 1291 885
BsmBI CGTCTCn'nnnn_
Cuts at: 799 2351 799
Size: 1552 6334
Bsp1286I G_dGCh'C
Cuts at: 833 3357 3675 4236 4350 4900 5511 5604 5678
Size: 2524 318 561 114 550 611 93 74
Cuts at: 5678 5868 5930 6728 833
Size: 190 62 798 1991
Fragments arranged by size:
2524 1991 798 611 561 550 318 190
114 93 74 62
BspHI T'CATG_A
Cuts at: 6318 6318
Size: 7886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 369 4722 4936 5150 5452 5833 369
Size: 4353 214 214 302 381 2422
Fragments arranged by size:
4353 2422 381 302 214 214
BsrBI CCG'CTC
Cuts at: 50 6178 6232 7716 7881 50
Size: 6128 54 1484 165 55
Fragments arranged by size:
6128 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4424 4501 5798 4424
Size: 77 1297 6512
Fragments arranged by size:
6512 1297 77
BsrFI r'CCGG_y
Cuts at: 5884 6065 5884
Size: 181 7705
BsrGI T'GTAC_A
Cuts at: 525 2583 2623 4440 525
Size: 2058 40 1817 3971
Fragments arranged by size:
3971 2058 1817 40
BssSI C'ACGA_G
Cuts at: 2969 3273 6157 6865 2969
Size: 304 2884 708 3990
Fragments arranged by size:
3990 2884 708 304
BstAPI GCAn_nnn'nTGC
Cuts at: 5263 5263
Size: 7886
BstBI TT'CG_AA
Cuts at: 4068 6247 4068
Size: 2179 5707
BstXI CCAn_nnnn'nTGG
Cuts at: 825 891 825
Size: 66 7820
BstYI r'GATC_y
Cuts at: 1 814 2007 2139 4375 4722 4911 5378 5401
Size: 813 1193 132 2236 347 189 467 23
Cuts at: 5401 5736 5982 6288 6300 6386 7438 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2236 1193 1052 813 467 449 347 335
306 246 189 132 86 23 12
BstZ17I GTA'TAC
Cuts at: 2542 2542
Size: 7886
Bsu36I CC'TnA_GG
Cuts at: 1897 1897
Size: 7886
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5836 6017 6180 5836
Size: 181 163 7542
Fragments arranged by size:
7542 181 163
BtsI GCAGTG_nn'
Cuts at: 1421 5129 1421
Size: 3708 4178
DraI TTT'AAA
Cuts at: 2717 3855 4821 4885 2717
Size: 1138 966 64 5718
Fragments arranged by size:
5718 1138 966 64
DraIII CAC_nnn'GTG
Cuts at: 237 237
Size: 7886
DrdI GACnn_nn'nnGTC
Cuts at: 5592 6936 5592
Size: 1344 6542
EaeI y'GGCC_r
Cuts at: 569 704 1245 5471 5645 6036 6063 7408 569
Size: 135 541 4226 174 391 27 1345 1047
Fragments arranged by size:
4226 1345 1047 541 391 174 135 27
EagI C'GGCC_G
Cuts at: 5471 5471
Size: 7886
EarI CTCTTCn'nnn_
Cuts at: 273 1294 2759 3386 3707 4022 4398 5909 6119
Size: 1021 1465 627 321 315 376 1511 210
Cuts at: 6119 273
Size: 2040
Fragments arranged by size:
2040 1511 1465 1021 627 376 321 315
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1516 6094 6836 6982 7696 1516
Size: 4578 742 146 714 1706
Fragments arranged by size:
4578 1706 742 714 146
EcoICRI GAG'CTC
Cuts at: 4234 4898 4234
Size: 664 7222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 1483 2165 2520 2783 2980 3031 3076 3976
Size: 885 682 355 263 197 51 45 900
Cuts at: 3976 4244 4487 4550 4721 5711 6143 6496 7158
Size: 268 243 63 171 990 432 353 662
Cuts at: 7158 598
Size: 1326
Fragments arranged by size:
1326 990 900 885 682 662 432 355
353 268 263 243 197 171 63 51
45
EcoNI CCTnn'n_nnAGG
Cuts at: 2815 2815
Size: 7886
EcoO109I rG'GnC_Cy
Cuts at: 5025 5025
Size: 7886
EcoRI G'AATT_C
Cuts at: 4890 4890
Size: 7886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 439 2755 3387 4006 5905 439
Size: 2316 632 619 1899 2420
Fragments arranged by size:
2420 2316 1899 632 619
FspI TGC'GCA
Cuts at: 4285 5667 4285
Size: 1382 6504
HaeII r_GCGC'y
Cuts at: 5320 5568 6798 7569 5320
Size: 248 1230 771 5637
Fragments arranged by size:
5637 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1057 1089 1975 2007 2290 2322 5728 5758 5760
Size: 32 886 32 283 32 3406 30 2
Cuts at: 5760 5790 1057
Size: 30 3153
Fragments arranged by size:
3406 3153 886 283 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 132 872 4925 132
Size: 740 4053 3093
Fragments arranged by size:
4053 3093 740
HindIII A'AGCT_T
Cuts at: 1517 3805 3963 3988 4258 1517
Size: 2288 158 25 270 5145
Fragments arranged by size:
5145 2288 270 158 25
HpaI GTT'AAC
Cuts at: 132 872 132
Size: 740 7146
KasI G'GCGC_C
Cuts at: 5316 5564 5316
Size: 248 7638
KpnI G_GTAC'C
Cuts at: 4906 4906
Size: 7886
MfeI C'AATT_G
Cuts at: 3497 4507 3497
Size: 1010 6876
MluI A'CGCG_T
Cuts at: 7319 7319
Size: 7886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 701 2480 6645 6829 701
Size: 1779 4165 184 1758
Fragments arranged by size:
4165 1779 1758 184
MscI TGG'CCA
Cuts at: 571 706 5647 571
Size: 135 4941 2810
Fragments arranged by size:
4941 2810 135
MslI CAynn'nnrTG
Cuts at: 1937 2619 5875 6002 1937
Size: 682 3256 127 3821
Fragments arranged by size:
3821 3256 682 127
MspA1I CmG'CkG
Cuts at: 4381 4479 4997 5311 5671 6455 6700 4381
Size: 98 518 314 360 784 245 5567
Fragments arranged by size:
5567 784 518 360 314 245 98
NaeI GCC'GGC
Cuts at: 6067 6067
Size: 7886
NarI GG'CG_CC
Cuts at: 5317 5565 5317
Size: 248 7638
NdeI CA'TA_TG
Cuts at: 4311 4457 4311
Size: 146 7740
NgoMIV G'CCGG_C
Cuts at: 6065 6065
Size: 7886
NheI G'CTAG_C
Cuts at: 1237 1237
Size: 7886
NsiI A_TGCA'T
Cuts at: 265 1326 265
Size: 1061 6825
NspI r_CATG'y
Cuts at: 199 901 1456 1942 4563 4939 5439 7042 199
Size: 702 555 486 2621 376 500 1603 1043
Fragments arranged by size:
2621 1603 1043 702 555 500 486 376
PasI CC'CwG_GG
Cuts at: 4757 4757
Size: 7886
PciI A'CATG_T
Cuts at: 897 1452 7038 897
Size: 555 5586 1745
Fragments arranged by size:
5586 1745 555
PflMI CCAn_nnn'nTGG
Cuts at: 240 240
Size: 7886
PfoI T'CCnGG_A
Cuts at: 3215 3215
Size: 7886
PmeI GTTT'AAAC
Cuts at: 4885 4885
Size: 7886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 815 847 6332 6364 815
Size: 32 5485 32 2337
Fragments arranged by size:
5485 2337 32 32
PshAI GACnn'nnGTC
Cuts at: 4548 4548
Size: 7886
PstI C_TGCA'G
Cuts at: 273 438 647 693 2157 2888 3647 4933 5618
Size: 165 209 46 1464 731 759 1286 685
Cuts at: 5618 273
Size: 2541
Fragments arranged by size:
2541 1464 1286 759 731 685 209 165
46
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7886
PvuII CAG'CTG
Cuts at: 5311 5671 5311
Size: 360 7526
RsrII CG'GwC_CG
Cuts at: 6081 6081
Size: 7886
SacI G_AGCT'C
Cuts at: 4236 4900 4236
Size: 664 7222
SalI G'TCGA_C
Cuts at: 4923 4923
Size: 7886
SapI GCTCTTCn'nnn_
Cuts at: 4022 5909 6119 4022
Size: 1887 210 5789
Fragments arranged by size:
5789 1887 210
SbfI CC_TGCA'GG
Cuts at: 4933 4933
Size: 7886
ScaI AGT'ACT
Cuts at: 960 7403 960
Size: 6443 1443
SexAI A'CCwGG_T
Cuts at: 233 233
Size: 7886
SfcI C'TryA_G
Cuts at: 33 269 291 434 643 689 1644 2153 2884
Size: 236 22 143 209 46 955 509 731
Cuts at: 2884 3643 4319 4929 5614 6582 6773 33
Size: 759 676 610 685 968 191 1146
Fragments arranged by size:
1146 968 955 759 731 685 676 610
509 236 209 191 143 46 22
SfoI GGC'GCC
Cuts at: 5318 5566 5318
Size: 248 7638
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7886
SmaI CCC'GGG
Cuts at: 4908 4908
Size: 7886
SmlI C'TyrA_G
Cuts at: 190 2812 3250 3742 3907 4243 5041 5956 6670
Size: 2622 438 492 165 336 798 915 714
Cuts at: 6670 6932 190
Size: 262 1144
Fragments arranged by size:
2622 1144 915 798 714 492 438 336
262 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7886
SphI G_CATG'C
Cuts at: 199 4939 5439 199
Size: 4740 500 2646
Fragments arranged by size:
4740 2646 500
SspI AAT'ATT
Cuts at: 4452 4452
Size: 7886
StyI C'CwwG_G
Cuts at: 549 1117 2068 3053 5020 5374 549
Size: 568 951 985 1967 354 3061
Fragments arranged by size:
3061 1967 985 951 568 354
SwaI ATTT'AAAT
Cuts at: 3855 3855
Size: 7886
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5469 5602 6061 6270 5469
Size: 133 459 209 7085
Fragments arranged by size:
7085 459 209 133
TatI w'GTAC_w
Cuts at: 525 958 1576 1592 1795 1941 2080 2583 2623
Size: 433 618 16 203 146 139 503 40
Cuts at: 2623 4174 4440 7401 525
Size: 1551 266 2961 1010
Fragments arranged by size:
2961 1551 1010 618 503 433 266 203
146 139 40 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4340 4372 7403 7435 4340
Size: 32 3031 32 4791
Fragments arranged by size:
4791 3031 32 32
Tth111I GACn'n_nGTC
Cuts at: 5683 5683
Size: 7886
XbaI T'CTAG_A
Cuts at: 4014 4747 4917 4014
Size: 733 170 6983
Fragments arranged by size:
6983 733 170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5381 5381
Size: 7886
XmaI C'CCGG_G
Cuts at: 4906 4906
Size: 7886
XmnI GAAnn'nnTTC
Cuts at: 539 2219 3830 3989 4451 539
Size: 1680 1611 159 462 3974
Fragments arranged by size:
3974 1680 1611 462 159
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AloI ApaI AscI
AvrII BbvCI BmgBI BsgI BsiWI BspEI BssHII BstEII
BtgI ClaI CspCI EcoRV FseI FspAI NcoI NotI
NruI PacI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI XhoI
ZraI