Restriction map for
pF1KA0188
(Circular) (Six-base) MAPSORT of: pF1KA0188.seq Check: 120 from: 1 to: 5783
pF1KA0188 5783 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1298 2645 2821 1298
Size: 1347 176 4260
Fragments arranged by size:
4260 1347 176
Acc65I G'GTAC_C
Cuts at: 2799 2799
Size: 5783
AclI AA'CG_TT
Cuts at: 5623 5623
Size: 5783
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1034 1349 2666 2691 3608 4040 4393 5055
Size: 406 315 1317 25 917 432 353 662
Cuts at: 5055 628
Size: 1356
Fragments arranged by size:
1356 1317 917 662 432 406 353 315
25
AflIII A'CryG_T
Cuts at: 647 3135 4935 5216 647
Size: 2488 1800 281 1214
Fragments arranged by size:
2488 1800 1214 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2362 2362
Size: 5783
AlwNI CAG_nnn'CTG
Cuts at: 3088 4526 3088
Size: 1438 4345
ApaI G_GGCC'C
Cuts at: 2360 2360
Size: 5783
ApaLI G'TGCA_C
Cuts at: 4621 4621
Size: 5783
ApoI r'AATT_y
Cuts at: 1007 2260 2787 5136 5392 1007
Size: 1253 527 2349 256 1398
Fragments arranged by size:
2349 1398 1253 527 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5783
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5783
AvaI C'yCGr_G
Cuts at: 287 1440 2803 287
Size: 1153 1363 3267
Fragments arranged by size:
3267 1363 1153
BamHI G'GATC_C
Cuts at: 1323 2808 1323
Size: 1485 4298
BanI G'GyrC_C
Cuts at: 2095 2799 3213 3461 3496 2095
Size: 704 414 248 35 4382
Fragments arranged by size:
4382 704 414 248 35
BanII G_rGCy'C
Cuts at: 161 478 670 1199 1404 2360 2797 3827 161
Size: 317 192 529 205 956 437 1030 2117
Fragments arranged by size:
2117 1030 956 529 437 317 205 192
BbeI G_GCGC'C
Cuts at: 3217 3465 3217
Size: 248 5535
BbsI GAAGACnn'nnnn_
Cuts at: 950 1151 1990 950
Size: 201 839 4743
Fragments arranged by size:
4743 839 201
BbvCI CC'TCA_GC
Cuts at: 1480 1522 2772 1480
Size: 42 1250 4491
Fragments arranged by size:
4491 1250 42
BciVI GTATCCnnnnn_n'
Cuts at: 2694 3681 4737 5783 2694
Size: 987 1056 1046 2694
Fragments arranged by size:
2694 1056 1046 987
BclI T'GATC_A
Cuts at: 390 3303 390
Size: 2913 2870
BfrBI ATG'CAT
Cuts at: 1552 1552
Size: 5783
BglI GCCn_nnn'nGGC
Cuts at: 2355 2355
Size: 5783
BglII A'GATC_T
Cuts at: 3298 5335 3298
Size: 2037 3746
BlpI GC'TnA_GC
Cuts at: 1870 2225 2895 1870
Size: 355 670 4758
Fragments arranged by size:
4758 670 355
Bme1580I G_kGCm'C
Cuts at: 1095 2098 2341 2360 3408 3501 4625 1095
Size: 1003 243 19 1048 93 1124 2253
Fragments arranged by size:
2253 1124 1048 1003 243 93 19
BmgBI CAC'GTC
Cuts at: 255 2046 255
Size: 1791 3992
BmrI ACTGGGnnnn_n'
Cuts at: 821 2124 2331 2995 3076 3411 5555 821
Size: 1303 207 664 81 335 2144 1049
Fragments arranged by size:
2144 1303 1049 664 335 207 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 684 1485 4150 684
Size: 801 2665 2317
Fragments arranged by size:
2665 2317 801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1171 1579 1890 712
Size: 459 408 311 4605
Fragments arranged by size:
4605 459 408 311
Bpu10I CC'TnA_GC
Cuts at: 1480 1522 2772 1480
Size: 42 1250 4491
Fragments arranged by size:
4491 1250 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2959 3838 4552 4850 2959
Size: 879 714 298 3892
Fragments arranged by size:
3892 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1147 5487 1147
Size: 4340 1443
BsaAI yAC'GTr
Cuts at: 117 2081 3136 117
Size: 1964 1055 2764
Fragments arranged by size:
2764 1964 1055
BsaBI GATnn'nnATC
Cuts at: 6 389 3297 5334 6
Size: 383 2908 2037 455
Fragments arranged by size:
2908 2037 455 383
BsaHI Gr'CG_yC
Cuts at: 541 1295 3214 3462 4164 5660 541
Size: 754 1919 248 702 1496 664
Fragments arranged by size:
1919 1496 754 702 664 248
BsaWI w'CCGG_w
Cuts at: 835 1369 1849 2972 3493 4171 4582 4729 5003
Size: 534 480 1123 521 678 411 147 274
Cuts at: 5003 835
Size: 1615
Fragments arranged by size:
1615 1123 678 534 521 480 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1230 1260 1658 1688 2573 2603 1230
Size: 30 398 30 885 30 4410
Fragments arranged by size:
4410 885 398 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 597 1207 1663 1930 597
Size: 610 456 267 4450
Fragments arranged by size:
4450 610 456 267
BseYI C'CCAG_C
Cuts at: 505 532 2374 3208 3966 4631 5039 505
Size: 27 1842 834 758 665 408 1249
Fragments arranged by size:
1842 1249 834 758 665 408 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2302 2380 2302
Size: 78 5705
BsiEI CG_ry'CG
Cuts at: 104 3325 3371 4601 104
Size: 3221 46 1230 1286
Fragments arranged by size:
3221 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 161 670 1199 1468 2797 3575 3765 4625 161
Size: 509 529 269 1329 778 190 860 1319
Fragments arranged by size:
1329 1319 860 778 529 509 269 190
BsmI GAATG_Cn'
Cuts at: 463 1552 463
Size: 1089 4694
BsmBI CGTCTCn'nnnn_
Cuts at: 532 532
Size: 5783
Bsp1286I G_dGCh'C
Cuts at: 161 478 670 1095 1199 1404 1468 2098 2341
Size: 317 192 425 104 205 64 630 243
Cuts at: 2341 2360 2797 3408 3501 3575 3765 3827 4625
Size: 19 437 611 93 74 190 62 798
Cuts at: 4625 161
Size: 1319
Fragments arranged by size:
1319 798 630 611 437 425 317 243
205 192 190 104 93 74 64 62
19
BspEI T'CCGG_A
Cuts at: 1369 1369
Size: 5783
BspHI T'CATG_A
Cuts at: 1730 4215 1730
Size: 2485 3298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2324 2833 3047 3349 3730 2324
Size: 509 214 302 381 4377
Fragments arranged by size:
4377 509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4075 4129 5613 5778 50
Size: 4025 54 1484 165 55
Fragments arranged by size:
4025 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1688 3695 1688
Size: 2007 3776
BsrFI r'CCGG_y
Cuts at: 130 3781 3962 130
Size: 3651 181 1951
Fragments arranged by size:
3651 1951 181
BssHII G'CGCG_C
Cuts at: 1920 1920
Size: 5783
BssSI C'ACGA_G
Cuts at: 1621 4054 4762 1621
Size: 2433 708 2642
Fragments arranged by size:
2642 2433 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 3160 1384
Size: 1776 4007
BstBI TT'CG_AA
Cuts at: 4144 4144
Size: 5783
BstEII G'GTnAC_C
Cuts at: 815 815
Size: 5783
BstXI CCAn_nnnn'nTGG
Cuts at: 226 1623 2046 2292 226
Size: 1397 423 246 3717
Fragments arranged by size:
3717 1397 423 246
BstYI r'GATC_y
Cuts at: 1 1323 1362 2808 3275 3298 3633 3879 4185
Size: 1322 39 1446 467 23 335 246 306
Cuts at: 4185 4197 4283 5335 1
Size: 12 86 1052 449
Fragments arranged by size:
1446 1322 1052 467 449 335 306 246
86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 746 1158 746
Size: 412 5371
BtgI C'CryG_G
Cuts at: 680 887 680
Size: 207 5576
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1482 2205 3733 3914 4077 1482
Size: 723 1528 181 163 3188
Fragments arranged by size:
3188 1528 723 181 163
BtsI GCAGTG_nn'
Cuts at: 1828 2734 3026 1828
Size: 906 292 4585
Fragments arranged by size:
4585 906 292
ClaI AT'CG_AT
Cuts at: 1607 1607
Size: 5783
DraI TTT'AAA
Cuts at: 2433 2782 2433
Size: 349 5434
DrdI GACnn_nn'nnGTC
Cuts at: 208 3489 4833 208
Size: 3281 1344 1158
Fragments arranged by size:
3281 1344 1158
EaeI y'GGCC_r
Cuts at: 132 416 1877 2175 3368 3542 3933 3960 5305
Size: 284 1461 298 1193 174 391 27 1345
Cuts at: 5305 132
Size: 610
Fragments arranged by size:
1461 1345 1193 610 391 298 284 174
27
EagI C'GGCC_G
Cuts at: 3368 3368
Size: 5783
EarI CTCTTCn'nnn_
Cuts at: 562 604 713 1964 2389 2622 3806 4016 562
Size: 42 109 1251 425 233 1184 210 2329
Fragments arranged by size:
2329 1251 1184 425 233 210 109 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 205 2311 3991 4733 4879 5593 205
Size: 2106 1680 742 146 714 395
Fragments arranged by size:
2106 1680 742 714 395 146
EcoICRI GAG'CTC
Cuts at: 159 668 1197 2795 159
Size: 509 529 1598 3147
Fragments arranged by size:
3147 1598 529 509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 712 1034 1171 1349 1579 1890 2666 2691
Size: 84 322 137 178 230 311 776 25
Cuts at: 2691 3608 4040 4393 5055 628
Size: 917 432 353 662 1356
Fragments arranged by size:
1356 917 776 662 432 353 322 311
230 178 137 84 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2197 2197
Size: 5783
EcoO109I rG'GnC_Cy
Cuts at: 276 1485 1518 2356 2357 2922 276
Size: 1209 33 838 1 565 3137
Fragments arranged by size:
3137 1209 838 565 33 1
EcoRI G'AATT_C
Cuts at: 2787 2787
Size: 5783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2696 3802 2696
Size: 1106 4677
FspI TGC'GCA
Cuts at: 3564 3564
Size: 5783
HaeII r_GCGC'y
Cuts at: 1174 3217 3465 4695 5466 1174
Size: 2043 248 1230 771 1491
Fragments arranged by size:
2043 1491 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 762 794 1230 1262 3625 3655 3657
Size: 32 201 32 436 32 2363 30 2
Cuts at: 3657 3687 529
Size: 30 2625
Fragments arranged by size:
2625 2363 436 201 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 303 2328 2571 2646 2822 303
Size: 2025 243 75 176 3264
Fragments arranged by size:
3264 2025 243 176 75
HindIII A'AGCT_T
Cuts at: 2016 2016
Size: 5783
KasI G'GCGC_C
Cuts at: 3213 3461 3213
Size: 248 5535
KpnI G_GTAC'C
Cuts at: 2803 2803
Size: 5783
MluI A'CGCG_T
Cuts at: 5216 5216
Size: 5783
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4542 4726 4542
Size: 184 5599
MscI TGG'CCA
Cuts at: 418 1879 2177 3544 418
Size: 1461 298 1367 2657
Fragments arranged by size:
2657 1461 1367 298
MslI CAynn'nnrTG
Cuts at: 661 1283 1318 2290 3772 3899 661
Size: 622 35 972 1482 127 2545
Fragments arranged by size:
2545 1482 972 622 127 35
MspA1I CmG'CkG
Cuts at: 465 597 1927 2087 2515 2776 2894 3208 3568
Size: 132 1330 160 428 261 118 314 360
Cuts at: 3568 4352 4597 465
Size: 784 245 1651
Fragments arranged by size:
1651 1330 784 428 360 314 261 245
160 132 118
NaeI GCC'GGC
Cuts at: 132 3964 132
Size: 3832 1951
NarI GG'CG_CC
Cuts at: 3214 3462 3214
Size: 248 5535
NcoI C'CATG_G
Cuts at: 680 680
Size: 5783
NdeI CA'TA_TG
Cuts at: 338 338
Size: 5783
NgoMIV G'CCGG_C
Cuts at: 130 3962 130
Size: 3832 1951
NsiI A_TGCA'T
Cuts at: 1554 1554
Size: 5783
NspI r_CATG'y
Cuts at: 651 1387 2602 2836 3336 4939 651
Size: 736 1215 234 500 1603 1495
Fragments arranged by size:
1603 1495 1215 736 500 234
PasI CC'CwG_GG
Cuts at: 2352 2352
Size: 5783
PciI A'CATG_T
Cuts at: 647 4935 647
Size: 4288 1495
PfoI T'CCnGG_A
Cuts at: 1252 1556 1252
Size: 304 5479
PmeI GTTT'AAAC
Cuts at: 2782 2782
Size: 5783
PmlI CAC'GTG
Cuts at: 2081 2081
Size: 5783
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 634 666 4229 4261 634
Size: 32 3563 32 2156
Fragments arranged by size:
3563 2156 32 32
PpuMI rG'GwC_Cy
Cuts at: 276 1485 276
Size: 1209 4574
PsiI TTA'TAA
Cuts at: 1649 1649
Size: 5783
PspOMI G'GGCC_C
Cuts at: 2356 2356
Size: 5783
PstI C_TGCA'G
Cuts at: 2830 3515 2830
Size: 685 5098
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5783
PvuII CAG'CTG
Cuts at: 465 597 1927 2515 3208 3568 465
Size: 132 1330 588 693 360 2680
Fragments arranged by size:
2680 1330 693 588 360 132
RsrII CG'GwC_CG
Cuts at: 3978 3978
Size: 5783
SacI G_AGCT'C
Cuts at: 161 670 1199 2797 161
Size: 509 529 1598 3147
Fragments arranged by size:
3147 1598 529 509
SalI G'TCGA_C
Cuts at: 2644 2820 2644
Size: 176 5607
SapI GCTCTTCn'nnn_
Cuts at: 3806 4016 3806
Size: 210 5573
SbfI CC_TGCA'GG
Cuts at: 2830 2830
Size: 5783
ScaI AGT'ACT
Cuts at: 5300 5300
Size: 5783
SfcI C'TryA_G
Cuts at: 33 557 2826 3511 4479 4670 33
Size: 524 2269 685 968 191 1146
Fragments arranged by size:
2269 1146 968 685 524 191
SfoI GGC'GCC
Cuts at: 3215 3463 3215
Size: 248 5535
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5783
SmaI CCC'GGG
Cuts at: 2805 2805
Size: 5783
SmlI C'TyrA_G
Cuts at: 2938 3853 4567 4829 2938
Size: 915 714 262 3892
Fragments arranged by size:
3892 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5783
SphI G_CATG'C
Cuts at: 2836 3336 2836
Size: 500 5283
SspI AAT'ATT
Cuts at: 1645 2561 1645
Size: 916 4867
StuI AGG'CCT
Cuts at: 497 1690 497
Size: 1193 4590
StyI C'CwwG_G
Cuts at: 680 2073 2193 2917 3271 680
Size: 1393 120 724 354 3192
Fragments arranged by size:
3192 1393 724 354 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 834 3366 3499 3958 4167 834
Size: 2532 133 459 209 2450
Fragments arranged by size:
2532 2450 459 209 133
TatI w'GTAC_w
Cuts at: 5298 5298
Size: 5783
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 796 813 828 845 1658 1690 5300 5332 796
Size: 17 15 17 813 32 3610 32 1247
Fragments arranged by size:
3610 1247 813 32 32 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 543 1294 3580 543
Size: 751 2286 2746
Fragments arranged by size:
2746 2286 751
XbaI T'CTAG_A
Cuts at: 2814 2814
Size: 5783
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 259 687 920 3278 114
Size: 145 428 233 2358 2619
Fragments arranged by size:
2619 2358 428 233 145
XmaI C'CCGG_G
Cuts at: 2803 2803
Size: 5783
XmnI GAAnn'nnTTC
Cuts at: 2676 2676
Size: 5783
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BcgI BmtI BsiWI BsrGI BstZ17I
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PflMI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI