Restriction map for
pF1KA0187
(Circular) (Six-base) MAPSORT of: pF1KA0187.seq Check: 7875 from: 1 to: 6959
pF1KA0187 6959 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3806 3806
Size: 6959
AccI GT'mk_AC
Cuts at: 3019 3997 3019
Size: 978 5981
Acc65I G'GTAC_C
Cuts at: 3975 3975
Size: 6959
AclI AA'CG_TT
Cuts at: 598 6799 598
Size: 6201 758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1481 1880 1898 1910 2905 3801 3867 3871
Size: 769 399 18 12 995 896 66 4
Cuts at: 3871 4784 5216 5963 6231 712
Size: 913 432 747 268 1440
Fragments arranged by size:
1440 995 913 896 769 747 432 399
268 66 18 12 4
AfeI AGC'GCT
Cuts at: 1084 1084
Size: 6959
AflIII A'CryG_T
Cuts at: 4311 5415 6392 4311
Size: 1104 977 4878
Fragments arranged by size:
4878 1104 977
AgeI A'CCGG_T
Cuts at: 5347 5347
Size: 6959
AhdI GACnn_n'nnGTC
Cuts at: 3483 3697 3483
Size: 214 6745
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3396 3428 3396
Size: 32 6927
AlwNI CAG_nnn'CTG
Cuts at: 725 1992 4264 5831 725
Size: 1267 2272 1567 1853
Fragments arranged by size:
2272 1853 1567 1267
ApaI G_GGCC'C
Cuts at: 1211 3061 1211
Size: 1850 5109
ApaLI G'TGCA_C
Cuts at: 2479 5729 2479
Size: 3250 3709
ApoI r'AATT_y
Cuts at: 995 2034 2701 2794 3212 3271 3887 3963 6312
Size: 1039 667 93 418 59 616 76 2349
Cuts at: 6312 6568 995
Size: 256 1386
Fragments arranged by size:
2349 1386 1039 667 616 418 256 93
76 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 6959
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6959
AvaI C'yCGr_G
Cuts at: 741 2921 3979 741
Size: 2180 1058 3721
Fragments arranged by size:
3721 2180 1058
BamHI G'GATC_C
Cuts at: 3984 3984
Size: 6959
BanI G'GyrC_C
Cuts at: 745 2095 3672 3794 3975 4389 4637 4672 745
Size: 1350 1577 122 181 414 248 35 3032
Fragments arranged by size:
3032 1577 1350 414 248 181 122 35
BanII G_rGCy'C
Cuts at: 347 1211 1642 2444 3061 3339 3973 5003 347
Size: 864 431 802 617 278 634 1030 2303
Fragments arranged by size:
2303 1030 864 802 634 617 431 278
BbeI G_GCGC'C
Cuts at: 4393 4641 4393
Size: 248 6711
BbsI GAAGACnn'nnnn_
Cuts at: 304 703 1589 1928 2120 2465 2993 3006 3387
Size: 399 886 339 192 345 528 13 381
Cuts at: 3387 304
Size: 3876
Fragments arranged by size:
3876 886 528 399 381 345 339 192
13
BbvCI CC'TCA_GC
Cuts at: 2135 3938 2135
Size: 1803 5156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 196 230 196
Size: 34 6925
BciVI GTATCCnnnnn_n'
Cuts at: 3422 4857 5618 6959 3422
Size: 1435 761 1341 3422
Fragments arranged by size:
3422 1435 1341 761
BclI T'GATC_A
Cuts at: 1530 4479 1530
Size: 2949 4010
BfrBI ATG'CAT
Cuts at: 3036 3036
Size: 6959
BglI GCCn_nnn'nGGC
Cuts at: 112 1267 112
Size: 1155 5804
BglII A'GATC_T
Cuts at: 549 4474 6511 549
Size: 3925 2037 997
Fragments arranged by size:
3925 2037 997
BlpI GC'TnA_GC
Cuts at: 1641 4071 1641
Size: 2430 4529
Bme1580I G_kGCm'C
Cuts at: 1211 2100 2483 3061 4584 4677 5733 1211
Size: 889 383 578 1523 93 1056 2437
Fragments arranged by size:
2437 1523 1056 889 578 383 93
BmgBI CAC'GTC
Cuts at: 2478 2478
Size: 6959
BmrI ACTGGGnnnn_n'
Cuts at: 2002 2349 3436 4171 4252 4587 6731 2002
Size: 347 1087 735 81 335 2144 2230
Fragments arranged by size:
2230 2144 1087 735 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1656 5326 1656
Size: 3670 3289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1148 1445 1757 1816 2295 3323 178
Size: 970 297 312 59 479 1028 3814
Fragments arranged by size:
3814 1028 970 479 312 297 59
Bpu10I CC'TnA_GC
Cuts at: 116 2135 3938 116
Size: 2019 1803 3137
Fragments arranged by size:
3137 2019 1803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 1651 1955 2086 2899 4135 5014 5506 5804
Size: 60 304 131 813 1236 879 492 298
Cuts at: 5804 1591
Size: 2746
Fragments arranged by size:
2746 1236 879 813 492 304 298 131
60
BsaI GGTCTCn'nnnn_
Cuts at: 2011 3684 6663 2011
Size: 1673 2979 2307
Fragments arranged by size:
2979 2307 1673
BsaAI yAC'GTr
Cuts at: 2871 4312 2871
Size: 1441 5518
BsaBI GATnn'nnATC
Cuts at: 6 4473 6510 6
Size: 4467 2037 455
Fragments arranged by size:
4467 2037 455
BsaHI Gr'CG_yC
Cuts at: 3527 4390 4638 5340 6836 3527
Size: 863 248 702 1496 3650
Fragments arranged by size:
3650 1496 863 702 248
BsaWI w'CCGG_w
Cuts at: 938 4148 4669 5347 5621 5768 6179 938
Size: 3210 521 678 274 147 411 1718
Fragments arranged by size:
3210 1718 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 652 682 652
Size: 30 6929
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 983 1522 1650 1890 2365 2452 2967 3850 3853
Size: 539 128 240 475 87 515 883 3
Cuts at: 3853 3856 983
Size: 3 4086
Fragments arranged by size:
4086 883 539 515 475 240 128 87
3 3
BseYI C'CCAG_C
Cuts at: 3426 4384 5142 5719 6215 3426
Size: 958 758 577 496 4170
Fragments arranged by size:
4170 958 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1680 1931 2821 2893 3583 3784 1680
Size: 251 890 72 690 201 4855
Fragments arranged by size:
4855 890 690 251 201 72
BsiEI CG_ry'CG
Cuts at: 104 3698 4501 4547 5755 104
Size: 3594 803 46 1208 1308
Fragments arranged by size:
3594 1308 1208 803 46
BsiHKAI G_wGCw'C
Cuts at: 397 975 1642 2444 2483 3339 3815 3973 4751
Size: 578 667 802 39 856 476 158 778
Cuts at: 4751 4941 5733 397
Size: 190 792 1623
Fragments arranged by size:
1623 856 802 792 778 667 578 476
190 158 39
BsmI GAATG_Cn'
Cuts at: 1391 3497 1391
Size: 2106 4853
BsmBI CGTCTCn'nnnn_
Cuts at: 197 197
Size: 6959
Bsp1286I G_dGCh'C
Cuts at: 347 397 975 1211 1642 2100 2444 2483 3061
Size: 50 578 236 431 458 344 39 578
Cuts at: 3061 3339 3815 3973 4584 4677 4751 4941 5003
Size: 278 476 158 611 93 74 190 62
Cuts at: 5003 5733 347
Size: 730 1573
Fragments arranged by size:
1573 730 611 578 578 476 458 431
344 278 236 190 158 93 74 62
50 39
BspEI T'CCGG_A
Cuts at: 6179 6179
Size: 6959
BspHI T'CATG_A
Cuts at: 3714 6135 3714
Size: 2421 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3806 3942 4009 4223 4525 4906 3806
Size: 136 67 214 302 381 5859
Fragments arranged by size:
5859 381 302 214 136 67
BsrBI CCG'CTC
Cuts at: 50 5251 5305 6789 6954 50
Size: 5201 54 1484 165 55
Fragments arranged by size:
5201 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1848 3036 4871 1848
Size: 1188 1835 3936
Fragments arranged by size:
3936 1835 1188
BsrFI r'CCGG_y
Cuts at: 3693 4957 5138 5347 3693
Size: 1264 181 209 5305
Fragments arranged by size:
5305 1264 209 181
BssSI C'ACGA_G
Cuts at: 5230 5588 5230
Size: 358 6601
BstAPI GCAn_nnn'nTGC
Cuts at: 4336 4336
Size: 6959
BstBI TT'CG_AA
Cuts at: 3291 5320 3291
Size: 2029 4930
BstXI CCAn_nnnn'nTGG
Cuts at: 2832 2832
Size: 6959
BstYI r'GATC_y
Cuts at: 1 189 549 2925 3725 3984 4451 4474 4809
Size: 188 360 2376 800 259 467 23 335
Cuts at: 4809 5055 6067 6153 6165 6511 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2376 1012 800 467 449 360 346 335
259 246 188 86 23 12
BstZ17I GTA'TAC
Cuts at: 3020 3020
Size: 6959
Bsu36I CC'TnA_GG
Cuts at: 3571 3868 3571
Size: 297 6662
BtgI C'CryG_G
Cuts at: 107 3500 3522 107
Size: 3393 22 3544
Fragments arranged by size:
3544 3393 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2878 4909 5090 5253 2878
Size: 2031 181 163 4584
Fragments arranged by size:
4584 2031 181 163
BtsI GCAGTG_nn'
Cuts at: 1686 1697 1986 3595 3733 4202 1686
Size: 11 289 1609 138 469 4443
Fragments arranged by size:
4443 1609 469 289 138 11
DraI TTT'AAA
Cuts at: 1091 3958 1091
Size: 2867 4092
DraIII CAC_nnn'GTG
Cuts at: 1909 1909
Size: 6959
DrdI GACnn_nn'nnGTC
Cuts at: 468 4665 5523 468
Size: 4197 858 1904
Fragments arranged by size:
4197 1904 858
EaeI y'GGCC_r
Cuts at: 3266 3695 4544 4718 5109 5136 6481 3266
Size: 429 849 174 391 27 1345 3744
Fragments arranged by size:
3744 1345 849 429 391 174 27
EagI C'GGCC_G
Cuts at: 3695 4544 3695
Size: 849 6110
EarI CTCTTCn'nnn_
Cuts at: 217 1067 1499 1682 1856 1931 2231 2587 3886
Size: 850 432 183 174 75 300 356 1299
Cuts at: 3886 4982 5192 217
Size: 1096 210 1984
Fragments arranged by size:
1984 1299 1096 850 432 356 300 210
183 174 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3702 5167 5477 5623 6769 3702
Size: 1465 310 146 1146 3892
Fragments arranged by size:
3892 1465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1640 2442 3337 3971 1640
Size: 802 895 634 4628
Fragments arranged by size:
4628 895 802 634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 712 1148 1445 1481 1757 1816 1880 1898
Size: 534 436 297 36 276 59 64 18
Cuts at: 1898 1910 2295 2905 3323 3801 3867 3871 4784
Size: 12 385 610 418 478 66 4 913
Cuts at: 4784 5216 5963 6231 178
Size: 432 747 268 906
Fragments arranged by size:
913 906 747 610 534 478 436 432
418 385 297 276 268 66 64 59
36 18 12 4
EcoNI CCTnn'n_nnAGG
Cuts at: 279 2145 279
Size: 1866 5093
EcoO109I rG'GnC_Cy
Cuts at: 373 454 1207 2328 2497 3057 3058 3624 3864
Size: 81 753 1121 169 560 1 566 240
Cuts at: 3864 4098 373
Size: 234 3234
Fragments arranged by size:
3234 1121 753 566 560 240 234 169
81 1
EcoRI G'AATT_C
Cuts at: 3887 3963 3887
Size: 76 6883
EcoRV GAT'ATC
Cuts at: 915 915
Size: 6959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3897 4978 3897
Size: 1081 5878
FspI TGC'GCA
Cuts at: 4740 4740
Size: 6959
HaeII r_GCGC'y
Cuts at: 1086 4393 4641 5663 6642 1086
Size: 3307 248 1022 979 1403
Fragments arranged by size:
3307 1403 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 360 392 516 548 1441 1473 1515 1547 1968
Size: 32 124 32 893 32 42 32 421
Cuts at: 1968 2000 2915 2947 3469 3501 3683 3715 4801
Size: 32 915 32 522 32 182 32 1086
Cuts at: 4801 4831 4833 4863 360
Size: 30 2 30 2456
Fragments arranged by size:
2456 1086 915 893 522 421 182 124
42 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 440 1170 2262 3998 440
Size: 730 1092 1736 3401
Fragments arranged by size:
3401 1736 1092 730
HindIII A'AGCT_T
Cuts at: 236 1954 2179 236
Size: 1718 225 5016
Fragments arranged by size:
5016 1718 225
KasI G'GCGC_C
Cuts at: 4389 4637 4389
Size: 248 6711
KpnI G_GTAC'C
Cuts at: 3979 3979
Size: 6959
MfeI C'AATT_G
Cuts at: 3946 3946
Size: 6959
MluI A'CGCG_T
Cuts at: 6392 6392
Size: 6959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 366 1372 1454 1556 5630 5814 366
Size: 1006 82 102 4074 184 1511
Fragments arranged by size:
4074 1511 1006 184 102 82
MscI TGG'CCA
Cuts at: 3268 4720 3268
Size: 1452 5507
MslI CAynn'nnrTG
Cuts at: 520 4948 5075 520
Size: 4428 127 2404
Fragments arranged by size:
4428 2404 127
MspA1I CmG'CkG
Cuts at: 1645 1858 2134 2688 3792 4070 4384 4744 5757
Size: 213 276 554 1104 278 314 360 1013
Cuts at: 5757 6002 1645
Size: 245 2602
Fragments arranged by size:
2602 1104 1013 554 360 314 278 276
245 213
NaeI GCC'GGC
Cuts at: 5140 5140
Size: 6959
NarI GG'CG_CC
Cuts at: 4390 4638 4390
Size: 248 6711
NcoI C'CATG_G
Cuts at: 107 3522 107
Size: 3415 3544
NgoMIV G'CCGG_C
Cuts at: 5138 5138
Size: 6959
NsiI A_TGCA'T
Cuts at: 3038 3038
Size: 6959
NspI r_CATG'y
Cuts at: 1005 3036 4012 4512 5419 1005
Size: 2031 976 500 907 2545
Fragments arranged by size:
2545 2031 976 907 500
PciI A'CATG_T
Cuts at: 5415 5415
Size: 6959
PflMI CCAn_nnn'nTGG
Cuts at: 385 810 1221 385
Size: 425 411 6123
Fragments arranged by size:
6123 425 411
PmeI GTTT'AAAC
Cuts at: 3958 3958
Size: 6959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3535 3567 6098 6130 3535
Size: 32 2531 32 4364
Fragments arranged by size:
4364 2531 32 32
PpuMI rG'GwC_Cy
Cuts at: 373 3864 373
Size: 3491 3468
PspOMI G'GGCC_C
Cuts at: 1207 3057 1207
Size: 1850 5109
PstI C_TGCA'G
Cuts at: 188 1704 4006 4691 188
Size: 1516 2302 685 2456
Fragments arranged by size:
2456 2302 1516 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6959
PvuII CAG'CTG
Cuts at: 1858 2134 2688 4384 4744 1858
Size: 276 554 1696 360 4073
Fragments arranged by size:
4073 1696 554 360 276
RsrII CG'GwC_CG
Cuts at: 5154 5154
Size: 6959
SacI G_AGCT'C
Cuts at: 1642 2444 3339 3973 1642
Size: 802 895 634 4628
Fragments arranged by size:
4628 895 802 634
SalI G'TCGA_C
Cuts at: 3996 3996
Size: 6959
SapI GCTCTTCn'nnn_
Cuts at: 1067 2231 3886 4982 5192 1067
Size: 1164 1655 1096 210 2834
Fragments arranged by size:
2834 1655 1164 1096 210
SbfI CC_TGCA'GG
Cuts at: 4006 4006
Size: 6959
ScaI AGT'ACT
Cuts at: 6476 6476
Size: 6959
SexAI A'CCwGG_T
Cuts at: 3481 3481
Size: 6959
SfcI C'TryA_G
Cuts at: 33 184 1700 2942 3137 4002 4687 5680 5871
Size: 151 1516 1242 195 865 685 993 191
Cuts at: 5871 33
Size: 1121
Fragments arranged by size:
1516 1242 1121 993 865 685 195 191
151
SfoI GGC'GCC
Cuts at: 4391 4639 4391
Size: 248 6711
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6959
SmaI CCC'GGG
Cuts at: 743 3981 743
Size: 3238 3721
SmlI C'TyrA_G
Cuts at: 1570 1630 1970 2101 2914 4114 5029 5521 5783
Size: 60 340 131 813 1200 915 492 262
Cuts at: 5783 1570
Size: 2746
Fragments arranged by size:
2746 1200 915 813 492 340 262 131
60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6959
SphI G_CATG'C
Cuts at: 4012 4512 4012
Size: 500 6459
StuI AGG'CCT
Cuts at: 843 2140 843
Size: 1297 5662
StyI C'CwwG_G
Cuts at: 107 1173 2417 3257 3522 3782 4093 4447 107
Size: 1066 1244 840 265 260 311 354 2619
Fragments arranged by size:
2619 1244 1066 840 354 311 265 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 457 497 673 1228 2824 3457 4542 4675 5134
Size: 40 176 555 1596 633 1085 133 459
Cuts at: 5134 5343 457
Size: 209 2073
Fragments arranged by size:
2073 1596 1085 633 555 459 209 176
133 40
TatI w'GTAC_w
Cuts at: 624 6474 624
Size: 5850 1109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 652 684 6476 6508 652
Size: 32 5792 32 1103
Fragments arranged by size:
5792 1103 32 32
Tth111I GACn'n_nGTC
Cuts at: 1159 4756 1159
Size: 3597 3362
XbaI T'CTAG_A
Cuts at: 2232 3990 2232
Size: 1758 5201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1218 2319 4454 1218
Size: 1101 2135 3723
Fragments arranged by size:
3723 2135 1101
XmaI C'CCGG_G
Cuts at: 741 3979 741
Size: 3238 3721
XmnI GAAnn'nnTTC
Cuts at: 2783 3880 2783
Size: 1097 5862
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI AscI AvrII BaeI BmtI
BsiWI BsrGI BssHII BstEII ClaI CspCI FseI FspAI
HpaI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI