Restriction map for
pF1KA0179
(Circular) (Six-base) MAPSORT of: pF1KA0179.seq Check: 3918 from: 1 to: 5333
pF1KA0179 5333 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1286 2259 1286
Size: 973 4360
AccI GT'mk_AC
Cuts at: 2371 2371
Size: 5333
Acc65I G'GTAC_C
Cuts at: 2349 2349
Size: 5333
AclI AA'CG_TT
Cuts at: 5173 5173
Size: 5333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 1079 1390 1966 2004 2251 3158 3590 4337
Size: 321 311 576 38 247 907 432 747
Cuts at: 4337 4605 758
Size: 268 1486
Fragments arranged by size:
1486 907 747 576 432 321 311 268
247 38
AflIII A'CryG_T
Cuts at: 1377 1541 2685 3789 4766 1377
Size: 164 1144 1104 977 1944
Fragments arranged by size:
1944 1144 1104 977 164
AgeI A'CCGG_T
Cuts at: 3721 3721
Size: 5333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1777 1777
Size: 5333
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1923 1955 1923
Size: 32 5301
AlwNI CAG_nnn'CTG
Cuts at: 153 1917 1959 2062 2638 4205 153
Size: 1764 42 103 576 1567 1281
Fragments arranged by size:
1764 1567 1281 576 103 42
ApaI G_GGCC'C
Cuts at: 935 1500 1784 2182 935
Size: 565 284 398 4086
Fragments arranged by size:
4086 565 398 284
ApaLI G'TGCA_C
Cuts at: 4103 4103
Size: 5333
ApoI r'AATT_y
Cuts at: 1042 2137 2337 4686 4942 1042
Size: 1095 200 2349 256 1433
Fragments arranged by size:
2349 1433 1095 256 200
AseI AT'TA_AT
Cuts at: 20 20
Size: 5333
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
AvaI C'yCGr_G
Cuts at: 692 1345 1491 1557 2186 2353 692
Size: 653 146 66 629 167 3672
Fragments arranged by size:
3672 653 629 167 146 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2142 2175 2142
Size: 33 5300
BamHI G'GATC_C
Cuts at: 2358 2358
Size: 5333
BanI G'GyrC_C
Cuts at: 2349 2763 3011 3046 2349
Size: 414 248 35 4636
Fragments arranged by size:
4636 414 248 35
BanII G_rGCy'C
Cuts at: 275 935 1346 1492 1500 1608 1784 1799 2182
Size: 660 411 146 8 108 176 15 383
Cuts at: 2182 2347 3377 275
Size: 165 1030 2231
Fragments arranged by size:
2231 1030 660 411 383 176 165 146
108 15 8
BbeI G_GCGC'C
Cuts at: 2767 3015 2767
Size: 248 5085
BbsI GAAGACnn'nnnn_
Cuts at: 226 693 766 850 1231 2152 2239 226
Size: 467 73 84 381 921 87 3320
Fragments arranged by size:
3320 921 467 381 87 84 73
BbvCI CC'TCA_GC
Cuts at: 791 1960 791
Size: 1169 4164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1375 1409 1375
Size: 34 5299
BciVI GTATCCnnnnn_n'
Cuts at: 825 3231 3992 5333 825
Size: 2406 761 1341 825
Fragments arranged by size:
2406 1341 825 761
BclI T'GATC_A
Cuts at: 2853 2853
Size: 5333
BglI GCCn_nnn'nGGC
Cuts at: 1372 1562 1677 1372
Size: 190 115 5028
Fragments arranged by size:
5028 190 115
BglII A'GATC_T
Cuts at: 2848 4885 2848
Size: 2037 3296
BlpI GC'TnA_GC
Cuts at: 2445 2445
Size: 5333
Bme1580I G_kGCm'C
Cuts at: 935 1500 1646 1784 2182 2958 3051 4107 935
Size: 565 146 138 398 776 93 1056 2161
Fragments arranged by size:
2161 1056 776 565 398 146 138 93
BmgBI CAC'GTC
Cuts at: 1406 1406
Size: 5333
BmrI ACTGGGnnnn_n'
Cuts at: 862 1424 2545 2626 2961 5105 862
Size: 562 1121 81 335 2144 1090
Fragments arranged by size:
2144 1121 1090 562 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 548 1446 1627 3700 548
Size: 898 181 2073 2181
Fragments arranged by size:
2181 2073 898 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1427 1813 1909 2039 925
Size: 502 386 96 130 4219
Fragments arranged by size:
4219 502 386 130 96
Bpu10I CC'TnA_GC
Cuts at: 791 1960 791
Size: 1169 4164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 840 2509 3388 3880 4178 607
Size: 233 1669 879 492 298 1762
Fragments arranged by size:
1762 1669 879 492 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 5037 5037
Size: 5333
BsaAI yAC'GTr
Cuts at: 1378 2686 1378
Size: 1308 4025
BsaBI GATnn'nnATC
Cuts at: 6 617 2847 4884 6
Size: 611 2230 2037 455
Fragments arranged by size:
2230 2037 611 455
BsaHI Gr'CG_yC
Cuts at: 157 2764 3012 3714 5210 157
Size: 2607 248 702 1496 280
Fragments arranged by size:
2607 1496 702 280 248
BsaWI w'CCGG_w
Cuts at: 2522 3043 3721 3995 4142 4553 2522
Size: 521 678 274 147 411 3302
Fragments arranged by size:
3302 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 2037 2067 499
Size: 30 1508 30 3765
Fragments arranged by size:
3765 1508 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 808 1435 808
Size: 627 4706
BseYI C'CCAG_C
Cuts at: 146 461 1748 1810 1900 2758 3516 4093 4589
Size: 315 1287 62 90 858 758 577 496
Cuts at: 4589 146
Size: 890
Fragments arranged by size:
1287 890 858 758 577 496 315 90
62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1415 1328
Size: 87 5246
BsiEI CG_ry'CG
Cuts at: 104 131 2875 2921 4129 104
Size: 27 2744 46 1208 1308
Fragments arranged by size:
2744 1308 1208 46 27
BsiHKAI G_wGCw'C
Cuts at: 275 1799 1877 2347 3125 3315 4107 275
Size: 1524 78 470 778 190 792 1501
Fragments arranged by size:
1524 1501 792 778 470 190 78
BsmBI CGTCTCn'nnnn_
Cuts at: 736 736
Size: 5333
Bsp1286I G_dGCh'C
Cuts at: 275 935 1346 1492 1500 1608 1646 1784 1799
Size: 660 411 146 8 108 38 138 15
Cuts at: 1799 1877 2182 2347 2958 3051 3125 3315 3377
Size: 78 305 165 611 93 74 190 62
Cuts at: 3377 4107 275
Size: 730 1501
Fragments arranged by size:
1501 730 660 611 411 305 190 165
146 138 108 93 78 74 62 38
15 8
BspEI T'CCGG_A
Cuts at: 4553 4553
Size: 5333
BspHI T'CATG_A
Cuts at: 4509 4509
Size: 5333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1286 2259 2383 2597 2899 3280 1286
Size: 973 124 214 302 381 3339
Fragments arranged by size:
3339 973 381 302 214 124
BsrBI CCG'CTC
Cuts at: 50 187 3625 3679 5163 5328 50
Size: 137 3438 54 1484 165 55
Fragments arranged by size:
3438 1484 165 137 55 54
BsrDI GCAATG_nn'
Cuts at: 284 1684 3245 284
Size: 1400 1561 2372
Fragments arranged by size:
2372 1561 1400
BsrFI r'CCGG_y
Cuts at: 126 1671 3331 3512 3721 126
Size: 1545 1660 181 209 1738
Fragments arranged by size:
1738 1660 1545 209 181
BssSI C'ACGA_G
Cuts at: 3604 3962 3604
Size: 358 4975
BstAPI GCAn_nnn'nTGC
Cuts at: 1278 2710 1278
Size: 1432 3901
BstBI TT'CG_AA
Cuts at: 704 3694 704
Size: 2990 2343
BstEII G'GTnAC_C
Cuts at: 778 778
Size: 5333
BstXI CCAn_nnnn'nTGG
Cuts at: 2316 2316
Size: 5333
BstYI r'GATC_y
Cuts at: 1 134 2358 2825 2848 3183 3429 4441 4527
Size: 133 2224 467 23 335 246 1012 86
Cuts at: 4527 4539 4885 1
Size: 12 346 449
Fragments arranged by size:
2224 1012 467 449 346 335 246 133
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 427 1574 427
Size: 1147 4186
BtgI C'CryG_G
Cuts at: 107 1477 2174 2220 107
Size: 1370 697 46 3220
Fragments arranged by size:
3220 1370 697 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3283 3464 3627 3283
Size: 181 163 4989
Fragments arranged by size:
4989 181 163
BtsI GCAGTG_nn'
Cuts at: 1529 1662 1932 2039 2576 1529
Size: 133 270 107 537 4286
Fragments arranged by size:
4286 537 270 133 107
ClaI AT'CG_AT
Cuts at: 632 632
Size: 5333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1638 1673 1850 1885 1638
Size: 35 177 35 5086
Fragments arranged by size:
5086 177 35 35
DraI TTT'AAA
Cuts at: 2332 2332
Size: 5333
DraIII CAC_nnn'GTG
Cuts at: 2226 2226
Size: 5333
DrdI GACnn_nn'nnGTC
Cuts at: 3039 3897 3039
Size: 858 4475
EaeI y'GGCC_r
Cuts at: 128 1382 1474 2270 2918 3092 3483 3510 4855
Size: 1254 92 796 648 174 391 27 1345
Cuts at: 4855 128
Size: 606
Fragments arranged by size:
1345 1254 796 648 606 391 174 92
27
EagI C'GGCC_G
Cuts at: 128 2918 128
Size: 2790 2543
EarI CTCTTCn'nnn_
Cuts at: 263 734 819 894 1214 1480 2019 3356 3566
Size: 471 85 75 320 266 539 1337 210
Cuts at: 3566 263
Size: 2030
Fragments arranged by size:
2030 1337 539 471 320 266 210 85
75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3541 3851 3997 5143 3541
Size: 310 146 1146 3731
Fragments arranged by size:
3731 1146 310 146
EcoICRI GAG'CTC
Cuts at: 273 1797 2345 273
Size: 1524 548 3261
Fragments arranged by size:
3261 1524 548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 925 1079 1390 1427 1813 1909 1966 2004
Size: 167 154 311 37 386 96 57 38
Cuts at: 2004 2039 2251 3158 3590 4337 4605 758
Size: 35 212 907 432 747 268 1486
Fragments arranged by size:
1486 907 747 432 386 311 268 212
167 154 96 57 38 37 35
EcoNI CCTnn'n_nnAGG
Cuts at: 502 1706 502
Size: 1204 4129
EcoO109I rG'GnC_Cy
Cuts at: 510 931 932 1496 1579 1589 1699 1711 1780
Size: 421 1 564 83 10 110 12 69
Cuts at: 1780 2059 2179 2472 510
Size: 279 120 293 3371
Fragments arranged by size:
3371 564 421 293 279 120 110 83
69 12 10 1
EcoRI G'AATT_C
Cuts at: 2137 2337 2137
Size: 200 5133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 504 2178 3352 504
Size: 1674 1174 2485
Fragments arranged by size:
2485 1674 1174
FspI TGC'GCA
Cuts at: 3114 3114
Size: 5333
HaeII r_GCGC'y
Cuts at: 1023 2767 3015 4037 5016 1023
Size: 1744 248 1022 979 1340
Fragments arranged by size:
1744 1340 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 514 546 565 597 675 707 1390 1422 1528
Size: 32 19 32 78 32 683 32 106
Cuts at: 1528 1551 1560 1583 3175 3205 3207 3237 514
Size: 23 9 23 1592 30 2 30 2610
Fragments arranged by size:
2610 1592 683 106 78 32 32 32
32 30 30 23 23 19 9 2
HincII GTy'rAC
Cuts at: 309 2372 309
Size: 2063 3270
HpaI GTT'AAC
Cuts at: 309 309
Size: 5333
KasI G'GCGC_C
Cuts at: 2763 3011 2763
Size: 248 5085
KpnI G_GTAC'C
Cuts at: 2353 2353
Size: 5333
MluI A'CGCG_T
Cuts at: 4766 4766
Size: 5333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1493 4004 4188 1493
Size: 2511 184 2638
Fragments arranged by size:
2638 2511 184
MscI TGG'CCA
Cuts at: 2272 3094 2272
Size: 822 4511
MslI CAynn'nnrTG
Cuts at: 214 3322 3449 214
Size: 3108 127 2098
Fragments arranged by size:
3108 2098 127
MspA1I CmG'CkG
Cuts at: 150 319 2444 2758 3118 4131 4376 150
Size: 169 2125 314 360 1013 245 1107
Fragments arranged by size:
2125 1107 1013 360 314 245 169
NaeI GCC'GGC
Cuts at: 128 1673 3514 128
Size: 1545 1841 1947
Fragments arranged by size:
1947 1841 1545
NarI GG'CG_CC
Cuts at: 2764 3012 2764
Size: 248 5085
NcoI C'CATG_G
Cuts at: 107 107
Size: 5333
NgoMIV G'CCGG_C
Cuts at: 126 1671 3512 126
Size: 1545 1841 1947
Fragments arranged by size:
1947 1841 1545
NspI r_CATG'y
Cuts at: 1545 2386 2886 3793 1545
Size: 841 500 907 3085
Fragments arranged by size:
3085 907 841 500
PasI CC'CwG_GG
Cuts at: 1300 2309 1300
Size: 1009 4324
PciI A'CATG_T
Cuts at: 1541 3789 1541
Size: 2248 3085
PflMI CCAn_nnn'nTGG
Cuts at: 153 675 1917 153
Size: 522 1242 3569
Fragments arranged by size:
3569 1242 522
PfoI T'CCnGG_A
Cuts at: 176 974 176
Size: 798 4535
PmeI GTTT'AAAC
Cuts at: 2332 2332
Size: 5333
PmlI CAC'GTG
Cuts at: 1378 1378
Size: 5333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4472 4504 4472
Size: 32 5301
PpuMI rG'GwC_Cy
Cuts at: 510 1579 1589 510
Size: 1069 10 4254
Fragments arranged by size:
4254 1069 10
PspOMI G'GGCC_C
Cuts at: 931 1496 1780 2178 931
Size: 565 284 398 4086
Fragments arranged by size:
4086 565 398 284
PstI C_TGCA'G
Cuts at: 1283 1766 2380 3065 1283
Size: 483 614 685 3551
Fragments arranged by size:
3551 685 614 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5333
PvuII CAG'CTG
Cuts at: 2758 3118 2758
Size: 360 4973
RsrII CG'GwC_CG
Cuts at: 3528 3528
Size: 5333
SacI G_AGCT'C
Cuts at: 275 1799 2347 275
Size: 1524 548 3261
Fragments arranged by size:
3261 1524 548
SalI G'TCGA_C
Cuts at: 2370 2370
Size: 5333
SanDI GG'GwC_CC
Cuts at: 1589 1589
Size: 5333
SapI GCTCTTCn'nnn_
Cuts at: 263 819 1480 2019 3356 3566 263
Size: 556 661 539 1337 210 2030
Fragments arranged by size:
2030 1337 661 556 539 210
SbfI CC_TGCA'GG
Cuts at: 2380 2380
Size: 5333
ScaI AGT'ACT
Cuts at: 4850 4850
Size: 5333
SfcI C'TryA_G
Cuts at: 33 262 1279 1762 2376 3061 4054 4245 33
Size: 229 1017 483 614 685 993 191 1121
Fragments arranged by size:
1121 1017 993 685 614 483 229 191
SfoI GGC'GCC
Cuts at: 2765 3013 2765
Size: 248 5085
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
SmaI CCC'GGG
Cuts at: 2355 2355
Size: 5333
SmlI C'TyrA_G
Cuts at: 622 819 2186 2488 3403 3895 4157 622
Size: 197 1367 302 915 492 262 1798
Fragments arranged by size:
1798 1367 915 492 302 262 197
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5333
SphI G_CATG'C
Cuts at: 2386 2886 2386
Size: 500 4833
StuI AGG'CCT
Cuts at: 1351 1351
Size: 5333
StyI C'CwwG_G
Cuts at: 107 2296 2467 2821 107
Size: 2189 171 354 2619
Fragments arranged by size:
2619 2189 354 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 197 760 2062 2916 3049 3508 3717 197
Size: 563 1302 854 133 459 209 1813
Fragments arranged by size:
1813 1302 854 563 459 209 133
TatI w'GTAC_w
Cuts at: 206 4848 206
Size: 4642 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 281 313 499 531 2037 2069 4850 4882 281
Size: 32 186 32 1506 32 2781 32 732
Fragments arranged by size:
2781 1506 732 186 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3130 3130
Size: 5333
XbaI T'CTAG_A
Cuts at: 2364 2364
Size: 5333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2262 2828 2262
Size: 566 4767
XhoI C'TCGA_G
Cuts at: 2186 2186
Size: 5333
XmaI C'CCGG_G
Cuts at: 2353 2353
Size: 5333
XmnI GAAnn'nnTTC
Cuts at: 257 257
Size: 5333
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BfrBI
BmtI BsiWI BsmI BsrGI BssHII BstZ17I EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI