Restriction map for
pF1KA0156
(Circular) (Six-base) MAPSORT of: pF1KA0156.seq Check: 4003 from: 1 to: 6002
pF1KA0156 6002 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 3040 3040
Size: 6002
Acc65I G'GTAC_C
Cuts at: 1259 3018 1259
Size: 1759 4243
AclI AA'CG_TT
Cuts at: 5842 5842
Size: 6002
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 563 1495 2191 2307 2371 3827 4259 5006
Size: 444 932 696 116 64 1456 432 747
Cuts at: 5006 5274 119
Size: 268 847
Fragments arranged by size:
1456 932 847 747 696 444 432 268
116 64
AflIII A'CryG_T
Cuts at: 611 1634 3354 4458 5435 611
Size: 1023 1720 1104 977 1178
Fragments arranged by size:
1720 1178 1104 1023 977
AgeI A'CCGG_T
Cuts at: 4390 4390
Size: 6002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1588 1588
Size: 6002
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1761 1793 1761
Size: 32 5970
AlwNI CAG_nnn'CTG
Cuts at: 981 2394 3307 4874 981
Size: 1413 913 1567 2109
Fragments arranged by size:
2109 1567 1413 913
ApaI G_GGCC'C
Cuts at: 167 2105 167
Size: 1938 4064
ApaLI G'TGCA_C
Cuts at: 1716 4772 1716
Size: 3056 2946
ApoI r'AATT_y
Cuts at: 3006 5355 5611 3006
Size: 2349 256 3397
Fragments arranged by size:
3397 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6002
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6002
AvaI C'yCGr_G
Cuts at: 142 346 1583 1919 3022 142
Size: 204 1237 336 1103 3122
Fragments arranged by size:
3122 1237 1103 336 204
BamHI G'GATC_C
Cuts at: 1148 3027 1148
Size: 1879 4123
BanI G'GyrC_C
Cuts at: 1026 1259 2581 2781 2949 3018 3432 3680 3715
Size: 233 1322 200 168 69 414 248 35
Cuts at: 3715 1026
Size: 3313
Fragments arranged by size:
3313 1322 414 248 233 200 168 69
35
BanII G_rGCy'C
Cuts at: 167 343 551 747 1668 1920 2105 2138 3016
Size: 176 208 196 921 252 185 33 878
Cuts at: 3016 4046 167
Size: 1030 2123
Fragments arranged by size:
2123 1030 921 878 252 208 196 185
176 33
BbeI G_GCGC'C
Cuts at: 3436 3684 3436
Size: 248 5754
BbsI GAAGACnn'nnnn_
Cuts at: 935 935
Size: 6002
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1564 1598 2028 2062 2333 2367 1564
Size: 34 430 34 271 34 5199
Fragments arranged by size:
5199 430 271 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3900 4661 6002 3900
Size: 761 1341 3900
Fragments arranged by size:
3900 1341 761
BclI T'GATC_A
Cuts at: 461 1290 3522 461
Size: 829 2232 2941
Fragments arranged by size:
2941 2232 829
BglI GCCn_nnn'nGGC
Cuts at: 2389 2947 2389
Size: 558 5444
BglII A'GATC_T
Cuts at: 3517 5554 3517
Size: 2037 3965
BlpI GC'TnA_GC
Cuts at: 3114 3114
Size: 6002
Bme1580I G_kGCm'C
Cuts at: 167 1720 2105 2865 3627 3720 4776 167
Size: 1553 385 760 762 93 1056 1393
Fragments arranged by size:
1553 1393 1056 762 760 385 93
BmgBI CAC'GTC
Cuts at: 1740 1740
Size: 6002
BmrI ACTGGGnnnn_n'
Cuts at: 1548 3214 3295 3630 5774 1548
Size: 1666 81 335 2144 1776
Fragments arranged by size:
2144 1776 1666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4369 4369
Size: 6002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 430 577 1003 1404 1453 2090 2416 415
Size: 15 147 426 401 49 637 326 4001
Fragments arranged by size:
4001 637 426 401 326 147 49 15
Bpu10I CC'TnA_GC
Cuts at: 472 2272 472
Size: 1800 4202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 859 3178 4057 4549 4847 736
Size: 123 2319 879 492 298 1891
Fragments arranged by size:
2319 1891 879 492 298 123
BsaI GGTCTCn'nnnn_
Cuts at: 2764 5706 2764
Size: 2942 3060
BsaAI yAC'GTr
Cuts at: 612 2358 2643 3355 612
Size: 1746 285 712 3259
Fragments arranged by size:
3259 1746 712 285
BsaBI GATnn'nnATC
Cuts at: 6 3516 5553 6
Size: 3510 2037 455
Fragments arranged by size:
3510 2037 455
BsaHI Gr'CG_yC
Cuts at: 2668 3433 3681 4383 5879 2668
Size: 765 248 702 1496 2791
Fragments arranged by size:
2791 1496 765 702 248
BsaWI w'CCGG_w
Cuts at: 2405 3191 3712 4390 4664 4811 5222 2405
Size: 786 521 678 274 147 411 3185
Fragments arranged by size:
3185 786 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 343 373 403 406 433 436 343
Size: 30 30 3 27 3 5909
Fragments arranged by size:
5909 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 391 394 427 1407 1450 2732 2879 196
Size: 195 3 33 980 43 1282 147 3319
Fragments arranged by size:
3319 1282 980 195 147 43 33 3
BseYI C'CCAG_C
Cuts at: 160 1679 2107 2934 3427 4185 4762 5258 160
Size: 1519 428 827 493 758 577 496 904
Fragments arranged by size:
1519 904 827 758 577 496 493 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 1900 2179 1684
Size: 216 279 5507
Fragments arranged by size:
5507 279 216
BsiEI CG_ry'CG
Cuts at: 104 122 3544 3590 4798 104
Size: 18 3422 46 1208 1308
Fragments arranged by size:
3422 1308 1208 46 18
BsiHKAI G_wGCw'C
Cuts at: 343 551 611 1668 1720 1739 1920 3016 3794
Size: 208 60 1057 52 19 181 1096 778
Cuts at: 3794 3984 4776 343
Size: 190 792 1569
Fragments arranged by size:
1569 1096 1057 792 778 208 190 181
60 52 19
BsmI GAATG_Cn'
Cuts at: 1091 1335 1091
Size: 244 5758
Bsp1286I G_dGCh'C
Cuts at: 167 343 551 611 747 1668 1720 1739 1920
Size: 176 208 60 136 921 52 19 181
Cuts at: 1920 2105 2138 2865 3016 3627 3720 3794 3984
Size: 185 33 727 151 611 93 74 190
Cuts at: 3984 4046 4776 167
Size: 62 730 1393
Fragments arranged by size:
1393 921 730 727 611 208 190 185
181 176 151 136 93 74 62 60
52 33 19
BspEI T'CCGG_A
Cuts at: 5222 5222
Size: 6002
BspHI T'CATG_A
Cuts at: 1604 5178 1604
Size: 3574 2428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2246 3052 3266 3568 3949 2246
Size: 806 214 302 381 4299
Fragments arranged by size:
4299 806 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4294 4348 5832 5997 50
Size: 4244 54 1484 165 55
Fragments arranged by size:
4244 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3914 3914
Size: 6002
BsrFI r'CCGG_y
Cuts at: 1333 4000 4181 4390 1333
Size: 2667 181 209 2945
Fragments arranged by size:
2945 2667 209 181
BsrGI T'GTAC_A
Cuts at: 1206 1206
Size: 6002
BssHII G'CGCG_C
Cuts at: 1110 1110
Size: 6002
BssSI C'ACGA_G
Cuts at: 1457 1829 2858 4273 4631 1457
Size: 372 1029 1415 358 2828
Fragments arranged by size:
2828 1415 1029 372 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3379 3379
Size: 6002
BstBI TT'CG_AA
Cuts at: 2143 4363 2143
Size: 2220 3782
BstEII G'GTnAC_C
Cuts at: 2604 2604
Size: 6002
BstXI CCAn_nnnn'nTGG
Cuts at: 1145 1145
Size: 6002
BstYI r'GATC_y
Cuts at: 1 1148 2309 3027 3494 3517 3852 4098 5110
Size: 1147 1161 718 467 23 335 246 1012
Cuts at: 5110 5196 5208 5554 1
Size: 86 12 346 449
Fragments arranged by size:
1161 1147 1012 718 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1390 2596 2891 1390
Size: 1206 295 4501
Fragments arranged by size:
4501 1206 295
BtgI C'CryG_G
Cuts at: 107 257 320 332 1271 1760 2331 2912 107
Size: 150 63 12 939 489 571 581 3197
Fragments arranged by size:
3197 939 581 571 489 150 63 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 317 2024 3952 4133 4296 317
Size: 1707 1928 181 163 2023
Fragments arranged by size:
2023 1928 1707 181 163
BtsI GCAGTG_nn'
Cuts at: 1683 3245 1683
Size: 1562 4440
ClaI AT'CG_AT
Cuts at: 1058 1058
Size: 6002
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1488 1523 1488
Size: 35 5967
DraI TTT'AAA
Cuts at: 2367 3001 2367
Size: 634 5368
DraIII CAC_nnn'GTG
Cuts at: 2788 2788
Size: 6002
DrdI GACnn_nn'nnGTC
Cuts at: 2844 3708 4566 2844
Size: 864 858 4280
Fragments arranged by size:
4280 864 858
EaeI y'GGCC_r
Cuts at: 119 236 675 1268 3587 3761 4152 4179 5524
Size: 117 439 593 2319 174 391 27 1345
Cuts at: 5524 119
Size: 597
Fragments arranged by size:
2319 1345 597 593 439 391 174 117
27
EagI C'GGCC_G
Cuts at: 119 3587 119
Size: 3468 2534
EarI CTCTTCn'nnn_
Cuts at: 368 539 1484 2015 2167 2287 2366 4025 4235
Size: 171 945 531 152 120 79 1659 210
Cuts at: 4235 368
Size: 2135
Fragments arranged by size:
2135 1659 945 531 210 171 152 120
79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4210 4520 4666 5812 4210
Size: 310 146 1146 4400
Fragments arranged by size:
4400 1146 310 146
EcoICRI GAG'CTC
Cuts at: 341 549 1666 1918 3014 341
Size: 208 1117 252 1096 3329
Fragments arranged by size:
3329 1117 1096 252 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 415 430 563 577 1003 1404 1453 1495
Size: 296 15 133 14 426 401 49 42
Cuts at: 1495 2090 2191 2307 2371 2416 3827 4259 5006
Size: 595 101 116 64 45 1411 432 747
Cuts at: 5006 5274 119
Size: 268 847
Fragments arranged by size:
1411 847 747 595 432 426 401 296
268 133 116 101 64 49 45 42
15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 790 790
Size: 6002
EcoO109I rG'GnC_Cy
Cuts at: 2101 2592 2631 3141 2101
Size: 491 39 510 4962
Fragments arranged by size:
4962 510 491 39
EcoRI G'AATT_C
Cuts at: 3006 3006
Size: 6002
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 505 730 1891 4021 505
Size: 225 1161 2130 2486
Fragments arranged by size:
2486 2130 1161 225
FspI TGC'GCA
Cuts at: 3783 3783
Size: 6002
HaeII r_GCGC'y
Cuts at: 2874 3436 3684 4706 5685 2874
Size: 562 248 1022 979 3191
Fragments arranged by size:
3191 1022 979 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 406 438 770 802 1497 1529 1539
Size: 32 238 32 332 32 695 32 10
Cuts at: 1539 1571 3844 3874 3876 3906 136
Size: 32 2273 30 2 30 2232
Fragments arranged by size:
2273 2232 695 332 238 32 32 32
32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 3041 3041
Size: 6002
HindIII A'AGCT_T
Cuts at: 1322 1322
Size: 6002
KasI G'GCGC_C
Cuts at: 3432 3680 3432
Size: 248 5754
KpnI G_GTAC'C
Cuts at: 1263 3022 1263
Size: 1759 4243
MluI A'CGCG_T
Cuts at: 5435 5435
Size: 6002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2337 4673 4857 2337
Size: 2336 184 3482
Fragments arranged by size:
3482 2336 184
MscI TGG'CCA
Cuts at: 677 1270 3763 677
Size: 593 2493 2916
Fragments arranged by size:
2916 2493 593
MslI CAynn'nnrTG
Cuts at: 614 2722 3991 4118 614
Size: 2108 1269 127 2498
Fragments arranged by size:
2498 2108 1269 127
MspA1I CmG'CkG
Cuts at: 241 334 393 981 2068 2107 2844 2914 3113
Size: 93 59 588 1087 39 737 70 199
Cuts at: 3113 3427 3787 4800 5045 241
Size: 314 360 1013 245 1198
Fragments arranged by size:
1198 1087 1013 737 588 360 314 245
199 93 70 59 39
NaeI GCC'GGC
Cuts at: 1335 4183 1335
Size: 2848 3154
NarI GG'CG_CC
Cuts at: 3433 3681 3433
Size: 248 5754
NcoI C'CATG_G
Cuts at: 107 257 320 1271 1760 107
Size: 150 63 951 489 4349
Fragments arranged by size:
4349 951 489 150 63
NdeI CA'TA_TG
Cuts at: 767 767
Size: 6002
NgoMIV G'CCGG_C
Cuts at: 1333 4181 1333
Size: 2848 3154
NspI r_CATG'y
Cuts at: 1575 1638 2184 2253 3055 3555 4462 1575
Size: 63 546 69 802 500 907 3115
Fragments arranged by size:
3115 907 802 546 500 69 63
PciI A'CATG_T
Cuts at: 1634 4458 1634
Size: 2824 3178
PflMI CCAn_nnn'nTGG
Cuts at: 1637 1637
Size: 6002
PmeI GTTT'AAAC
Cuts at: 3001 3001
Size: 6002
PmlI CAC'GTG
Cuts at: 612 612
Size: 6002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5141 5173 5141
Size: 32 5970
PpuMI rG'GwC_Cy
Cuts at: 2592 2592
Size: 6002
PspOMI G'GGCC_C
Cuts at: 163 2101 163
Size: 1938 4064
PstI C_TGCA'G
Cuts at: 1860 2067 2870 2886 2925 3049 3734 1860
Size: 207 803 16 39 124 685 4128
Fragments arranged by size:
4128 803 685 207 124 39 16
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6002
PvuII CAG'CTG
Cuts at: 393 981 2068 2844 3427 3787 393
Size: 588 1087 776 583 360 2608
Fragments arranged by size:
2608 1087 776 588 583 360
RsrII CG'GwC_CG
Cuts at: 4197 4197
Size: 6002
SacI G_AGCT'C
Cuts at: 343 551 1668 1920 3016 343
Size: 208 1117 252 1096 3329
Fragments arranged by size:
3329 1117 1096 252 208
SacII CC_GC'GG
Cuts at: 335 2915 335
Size: 2580 3422
SalI G'TCGA_C
Cuts at: 3039 3039
Size: 6002
SapI GCTCTTCn'nnn_
Cuts at: 539 1484 2015 4025 4235 539
Size: 945 531 2010 210 2306
Fragments arranged by size:
2306 2010 945 531 210
SbfI CC_TGCA'GG
Cuts at: 3049 3049
Size: 6002
ScaI AGT'ACT
Cuts at: 682 5519 682
Size: 4837 1165
SexAI A'CCwGG_T
Cuts at: 2784 2784
Size: 6002
SfcI C'TryA_G
Cuts at: 33 973 1856 2063 2244 2866 2882 2921 3045
Size: 940 883 207 181 622 16 39 124
Cuts at: 3045 3730 4723 4914 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 940 883 685 622 207 191
181 124 39 16
SfoI GGC'GCC
Cuts at: 3434 3682 3434
Size: 248 5754
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6002
SmaI CCC'GGG
Cuts at: 348 3024 348
Size: 2676 3326
SmlI C'TyrA_G
Cuts at: 715 838 3157 4072 4564 4826 715
Size: 123 2319 915 492 262 1891
Fragments arranged by size:
2319 1891 915 492 262 123
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6002
SphI G_CATG'C
Cuts at: 2253 3055 3555 2253
Size: 802 500 4700
Fragments arranged by size:
4700 802 500
StuI AGG'CCT
Cuts at: 2290 2528 2290
Size: 238 5764
StyI C'CwwG_G
Cuts at: 107 155 167 257 320 494 1030 1271 1463
Size: 48 12 90 63 174 536 241 192
Cuts at: 1463 1760 2186 3136 3490 107
Size: 297 426 950 354 2619
Fragments arranged by size:
2619 950 536 426 354 297 241 192
174 90 63 48 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1693 2967 3585 3718 4177 4386 1693
Size: 1274 618 133 459 209 3309
Fragments arranged by size:
3309 1274 618 459 209 133
TatI w'GTAC_w
Cuts at: 680 1206 2542 5517 680
Size: 526 1336 2975 1165
Fragments arranged by size:
2975 1336 1165 526
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 343 375 5519 5551 343
Size: 32 5144 32 794
Fragments arranged by size:
5144 794 32 32
Tth111I GACn'n_nGTC
Cuts at: 3799 3799
Size: 6002
XbaI T'CTAG_A
Cuts at: 3033 3033
Size: 6002
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 685 1356 1428 3497 501
Size: 184 671 72 2069 3006
Fragments arranged by size:
3006 2069 671 184 72
XmaI C'CCGG_G
Cuts at: 346 3022 346
Size: 2676 3326
XmnI GAAnn'nnTTC
Cuts at: 530 530
Size: 6002
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmBI BstZ17I EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PfoI PshAI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI