Restriction map for
pF1KA0151
(Circular) (Six-base) MAPSORT of: pF1KA0151.seq Check: 2240 from: 1 to: 5261
pF1KA0151 5261 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 252 491 838 913 1866 1976 252
Size: 239 347 75 953 110 3537
Fragments arranged by size:
3537 953 347 239 110 75
AatII G_ACGT'C
Cuts at: 1304 1304
Size: 5261
AccI GT'mk_AC
Cuts at: 2299 2299
Size: 5261
Acc65I G'GTAC_C
Cuts at: 2277 2277
Size: 5261
AclI AA'CG_TT
Cuts at: 5101 5101
Size: 5261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1236 1266 1594 2053 3086 3518 4265 4533 1236
Size: 30 328 459 1033 432 747 268 1964
Fragments arranged by size:
1964 1033 747 459 432 328 268 30
AflIII A'CryG_T
Cuts at: 656 2613 3717 4694 656
Size: 1957 1104 977 1223
Fragments arranged by size:
1957 1223 1104 977
AgeI A'CCGG_T
Cuts at: 3649 3649
Size: 5261
AleI CACnn'nnGTG
Cuts at: 1181 1181
Size: 5261
AlwNI CAG_nnn'CTG
Cuts at: 754 1383 1572 2566 4133 754
Size: 629 189 994 1567 1882
Fragments arranged by size:
1882 1567 994 629 189
ApaI G_GGCC'C
Cuts at: 875 1401 875
Size: 526 4735
ApaLI G'TGCA_C
Cuts at: 1123 1948 4031 1123
Size: 825 2083 2353
Fragments arranged by size:
2353 2083 825
ApoI r'AATT_y
Cuts at: 1845 2265 4614 4870 1845
Size: 420 2349 256 2236
Fragments arranged by size:
2349 2236 420 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5261
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
AvaI C'yCGr_G
Cuts at: 590 1189 2281 590
Size: 599 1092 3570
Fragments arranged by size:
3570 1092 599
BamHI G'GATC_C
Cuts at: 2286 2286
Size: 5261
BanI G'GyrC_C
Cuts at: 850 939 1366 2277 2691 2939 2974 850
Size: 89 427 911 414 248 35 3137
Fragments arranged by size:
3137 911 427 414 248 89 35
BanII G_rGCy'C
Cuts at: 409 875 1196 1401 2091 2165 2275 3305 409
Size: 466 321 205 690 74 110 1030 2365
Fragments arranged by size:
2365 1030 690 466 321 205 110 74
BbeI G_GCGC'C
Cuts at: 1370 2695 2943 1370
Size: 1325 248 3688
Fragments arranged by size:
3688 1325 248
BbsI GAAGACnn'nnnn_
Cuts at: 216 1038 216
Size: 822 4439
BbvCI CC'TCA_GC
Cuts at: 1510 1510
Size: 5261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5227
BciVI GTATCCnnnnn_n'
Cuts at: 3159 3920 5261 3159
Size: 761 1341 3159
Fragments arranged by size:
3159 1341 761
BclI T'GATC_A
Cuts at: 2781 2781
Size: 5261
BglI GCCn_nnn'nGGC
Cuts at: 1979 1979
Size: 5261
BglII A'GATC_T
Cuts at: 2776 4813 2776
Size: 2037 3224
BlpI GC'TnA_GC
Cuts at: 2373 2373
Size: 5261
Bme1580I G_kGCm'C
Cuts at: 855 875 944 1127 1401 1952 2886 2979 4035
Size: 20 69 183 274 551 934 93 1056
Cuts at: 4035 855
Size: 2081
Fragments arranged by size:
2081 1056 934 551 274 183 93 69
20
BmrI ACTGGGnnnn_n'
Cuts at: 928 1449 2473 2554 2889 5033 928
Size: 521 1024 81 335 2144 1156
Fragments arranged by size:
2144 1156 1024 521 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 390 1641 3628 390
Size: 1251 1987 2023
Fragments arranged by size:
2023 1987 1251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 421 741 898 982 1441 1611 360
Size: 61 320 157 84 459 170 4010
Fragments arranged by size:
4010 459 320 170 157 84 61
Bpu10I CC'TnA_GC
Cuts at: 1510 1666 2090 1510
Size: 156 424 4681
Fragments arranged by size:
4681 424 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2437 3316 3808 4106 2437
Size: 879 492 298 3592
Fragments arranged by size:
3592 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1003 1645 1948 4965 1003
Size: 642 303 3017 1299
Fragments arranged by size:
3017 1299 642 303
BsaAI yAC'GTr
Cuts at: 2614 2614
Size: 5261
BsaBI GATnn'nnATC
Cuts at: 6 2775 4812 6
Size: 2769 2037 455
Fragments arranged by size:
2769 2037 455
BsaHI Gr'CG_yC
Cuts at: 1301 1367 2692 2940 3642 5138 1301
Size: 66 1325 248 702 1496 1424
Fragments arranged by size:
1496 1424 1325 702 248 66
BsaWI w'CCGG_w
Cuts at: 199 812 2450 2971 3649 3923 4070 4481 199
Size: 613 1638 521 678 274 147 411 979
Fragments arranged by size:
1638 979 678 613 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 194 224 793 823 1408 1438 194
Size: 30 569 30 585 30 4017
Fragments arranged by size:
4017 585 569 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 562 652 1492 1831 1903 343
Size: 219 90 840 339 72 3701
Fragments arranged by size:
3701 840 339 219 90 72
BseYI C'CCAG_C
Cuts at: 150 686 835 983 1195 2122 2238 2686 3444
Size: 536 149 148 212 927 116 448 758
Cuts at: 3444 4021 4517 150
Size: 577 496 894
Fragments arranged by size:
927 894 758 577 536 496 448 212
149 148 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 1054 1420 1918 280
Size: 774 366 498 3623
Fragments arranged by size:
3623 774 498 366
BsiEI CG_ry'CG
Cuts at: 104 2803 2849 4057 104
Size: 2699 46 1208 1308
Fragments arranged by size:
2699 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 118 1127 1457 1952 2165 2275 3053 3243 4035
Size: 1009 330 495 213 110 778 190 792
Cuts at: 4035 118
Size: 1344
Fragments arranged by size:
1344 1009 792 778 495 330 213 190
110
BsmI GAATG_Cn'
Cuts at: 420 420
Size: 5261
BsmBI CGTCTCn'nnnn_
Cuts at: 325 624 1206 325
Size: 299 582 4380
Fragments arranged by size:
4380 582 299
Bsp1286I G_dGCh'C
Cuts at: 118 409 855 875 944 1127 1196 1401 1457
Size: 291 446 20 69 183 69 205 56
Cuts at: 1457 1952 2091 2165 2275 2886 2979 3053 3243
Size: 495 139 74 110 611 93 74 190
Cuts at: 3243 3305 4035 118
Size: 62 730 1344
Fragments arranged by size:
1344 730 611 495 446 291 205 190
183 139 110 93 74 74 69 69
62 56 20
BspEI T'CCGG_A
Cuts at: 199 4481 199
Size: 4282 979
BspHI T'CATG_A
Cuts at: 4437 4437
Size: 5261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 251 252 491 653 838 913 1337 1866
Size: 96 1 239 162 185 75 424 529
Cuts at: 1866 1976 2150 2311 2525 2827 3208 155
Size: 110 174 161 214 302 381 2208
Fragments arranged by size:
2208 529 424 381 302 239 214 185
174 162 161 110 96 75 1
BsrBI CCG'CTC
Cuts at: 50 666 3553 3607 5091 5256 50
Size: 616 2887 54 1484 165 55
Fragments arranged by size:
2887 1484 616 165 55 54
BsrDI GCAATG_nn'
Cuts at: 842 3173 842
Size: 2331 2930
BsrFI r'CCGG_y
Cuts at: 344 469 831 1369 3259 3440 3649 344
Size: 125 362 538 1890 181 209 1956
Fragments arranged by size:
1956 1890 538 362 209 181 125
BsrGI T'GTAC_A
Cuts at: 176 176
Size: 5261
BssSI C'ACGA_G
Cuts at: 1951 3532 3890 1951
Size: 1581 358 3322
Fragments arranged by size:
3322 1581 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2638 2638
Size: 5261
BstBI TT'CG_AA
Cuts at: 678 3622 678
Size: 2944 2317
BstEII G'GTnAC_C
Cuts at: 1174 1174
Size: 5261
BstXI CCAn_nnnn'nTGG
Cuts at: 960 960
Size: 5261
BstYI r'GATC_y
Cuts at: 1 714 1562 2286 2753 2776 3111 3357 4369
Size: 713 848 724 467 23 335 246 1012
Cuts at: 4369 4455 4467 4813 1
Size: 86 12 346 449
Fragments arranged by size:
1012 848 724 713 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 431 1268 431
Size: 837 4424
BtgI C'CryG_G
Cuts at: 787 821 787
Size: 34 5227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 490 1200 3211 3392 3555 490
Size: 710 2011 181 163 2196
Fragments arranged by size:
2196 2011 710 181 163
BtsI GCAGTG_nn'
Cuts at: 164 1346 2504 164
Size: 1182 1158 2921
Fragments arranged by size:
2921 1182 1158
DraI TTT'AAA
Cuts at: 2260 2260
Size: 5261
DrdI GACnn_nn'nnGTC
Cuts at: 2967 3825 2967
Size: 858 4403
EaeI y'GGCC_r
Cuts at: 467 950 2846 3020 3411 3438 4783 467
Size: 483 1896 174 391 27 1345 945
Fragments arranged by size:
1896 1345 945 483 391 174 27
EagI C'GGCC_G
Cuts at: 2846 2846
Size: 5261
EarI CTCTTCn'nnn_
Cuts at: 1255 1687 2042 3284 3494 1255
Size: 432 355 1242 210 3022
Fragments arranged by size:
3022 1242 432 355 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 353 807 1351 3469 3779 3925 5071 353
Size: 454 544 2118 310 146 1146 543
Fragments arranged by size:
2118 1146 544 543 454 310 146
EcoICRI GAG'CTC
Cuts at: 2163 2273 2163
Size: 110 5151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 421 741 898 982 1236 1266 1441 1594
Size: 61 320 157 84 254 30 175 153
Cuts at: 1594 1611 2053 3086 3518 4265 4533 360
Size: 17 442 1033 432 747 268 1088
Fragments arranged by size:
1088 1033 747 442 432 320 268 254
175 157 153 84 61 30 17
EcoNI CCTnn'n_nnAGG
Cuts at: 1057 1168 1057
Size: 111 5150
EcoO109I rG'GnC_Cy
Cuts at: 279 872 1287 1380 1398 1620 2139 2400 279
Size: 593 415 93 18 222 519 261 3140
Fragments arranged by size:
3140 593 519 415 261 222 93 18
EcoRI G'AATT_C
Cuts at: 2265 2265
Size: 5261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1776 3280 1776
Size: 1504 3757
FspI TGC'GCA
Cuts at: 3042 3042
Size: 5261
HaeII r_GCGC'y
Cuts at: 587 1370 2695 2943 3965 4944 587
Size: 783 1325 248 1022 979 904
Fragments arranged by size:
1325 1022 979 904 783 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 699 731 1022 1054 1686 1718 3103 3133 3135
Size: 32 291 32 632 32 1385 30 2
Cuts at: 3135 3165 699
Size: 30 2795
Fragments arranged by size:
2795 1385 632 291 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2300 2300
Size: 5261
KasI G'GCGC_C
Cuts at: 1366 2691 2939 1366
Size: 1325 248 3688
Fragments arranged by size:
3688 1325 248
KpnI G_GTAC'C
Cuts at: 2281 2281
Size: 5261
MluI A'CGCG_T
Cuts at: 4694 4694
Size: 5261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3932 4116 3932
Size: 184 5077
MscI TGG'CCA
Cuts at: 952 3022 952
Size: 2070 3191
MslI CAynn'nnrTG
Cuts at: 140 730 1181 1450 3250 3377 140
Size: 590 451 269 1800 127 2024
Fragments arranged by size:
2024 1800 590 451 269 127
MspA1I CmG'CkG
Cuts at: 912 936 2372 2686 3046 4059 4304 912
Size: 24 1436 314 360 1013 245 1869
Fragments arranged by size:
1869 1436 1013 360 314 245 24
NaeI GCC'GGC
Cuts at: 346 471 833 1371 3442 346
Size: 125 362 538 2071 2165
Fragments arranged by size:
2165 2071 538 362 125
NarI GG'CG_CC
Cuts at: 1367 2692 2940 1367
Size: 1325 248 3688
Fragments arranged by size:
3688 1325 248
NgoMIV G'CCGG_C
Cuts at: 344 469 831 1369 3440 344
Size: 125 362 538 2071 2165
Fragments arranged by size:
2165 2071 538 362 125
NruI TCG'CGA
Cuts at: 509 509
Size: 5261
NspI r_CATG'y
Cuts at: 660 1469 2157 2314 2814 3721 660
Size: 809 688 157 500 907 2200
Fragments arranged by size:
2200 907 809 688 500 157
PasI CC'CwG_GG
Cuts at: 2013 2013
Size: 5261
PciI A'CATG_T
Cuts at: 656 3717 656
Size: 3061 2200
PflMI CCAn_nnn'nTGG
Cuts at: 983 1804 983
Size: 821 4440
PfoI T'CCnGG_A
Cuts at: 959 1112 1565 1883 2021 2042 2198 959
Size: 153 453 318 138 21 156 4022
Fragments arranged by size:
4022 453 318 156 153 138 21
PmeI GTTT'AAAC
Cuts at: 2260 2260
Size: 5261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 424 456 4400 4432 424
Size: 32 3944 32 1253
Fragments arranged by size:
3944 1253 32 32
PpuMI rG'GwC_Cy
Cuts at: 279 1287 1620 2139 279
Size: 1008 333 519 3401
Fragments arranged by size:
3401 1008 519 333
PspOMI G'GGCC_C
Cuts at: 871 1397 871
Size: 526 4735
PspXI vC'TCGA_Gb
Cuts at: 1189 1189
Size: 5261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1750 1782 1750
Size: 32 5229
PstI C_TGCA'G
Cuts at: 929 1028 1334 1587 1877 2308 2993 929
Size: 99 306 253 290 431 685 3197
Fragments arranged by size:
3197 685 431 306 290 253 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5261
PvuII CAG'CTG
Cuts at: 912 936 2686 3046 912
Size: 24 1750 360 3127
Fragments arranged by size:
3127 1750 360 24
RsrII CG'GwC_CG
Cuts at: 3456 3456
Size: 5261
SacI G_AGCT'C
Cuts at: 2165 2275 2165
Size: 110 5151
SalI G'TCGA_C
Cuts at: 2298 2298
Size: 5261
SanDI GG'GwC_CC
Cuts at: 1287 1287
Size: 5261
SapI GCTCTTCn'nnn_
Cuts at: 1687 2042 3284 3494 1687
Size: 355 1242 210 3454
Fragments arranged by size:
3454 1242 355 210
SbfI CC_TGCA'GG
Cuts at: 1334 1877 2308 1334
Size: 543 431 4287
Fragments arranged by size:
4287 543 431
ScaI AGT'ACT
Cuts at: 370 4778 370
Size: 4408 853
SfcI C'TryA_G
Cuts at: 33 925 1024 1330 1583 1873 2304 2989 3982
Size: 892 99 306 253 290 431 685 993
Cuts at: 3982 4173 33
Size: 191 1121
Fragments arranged by size:
1121 993 892 685 431 306 290 253
191 99
SfoI GGC'GCC
Cuts at: 1368 2693 2941 1368
Size: 1325 248 3688
Fragments arranged by size:
3688 1325 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5261
SmaI CCC'GGG
Cuts at: 592 2283 592
Size: 1691 3570
SmlI C'TyrA_G
Cuts at: 1189 2416 3331 3823 4085 1189
Size: 1227 915 492 262 2365
Fragments arranged by size:
2365 1227 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5261
SphI G_CATG'C
Cuts at: 2314 2814 2314
Size: 500 4761
StuI AGG'CCT
Cuts at: 2095 2095
Size: 5261
StyI C'CwwG_G
Cuts at: 1352 2395 2749 1352
Size: 1043 354 3864
Fragments arranged by size:
3864 1043 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 612 2090 2844 2977 3436 3645 612
Size: 1478 754 133 459 209 2228
Fragments arranged by size:
2228 1478 754 459 209 133
TatI w'GTAC_w
Cuts at: 176 368 4776 176
Size: 192 4408 661
Fragments arranged by size:
4408 661 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4778 4810 4778
Size: 32 5229
Tth111I GACn'n_nGTC
Cuts at: 3058 3058
Size: 5261
XbaI T'CTAG_A
Cuts at: 2292 2292
Size: 5261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 751 1359 2756 751
Size: 608 1397 3256
Fragments arranged by size:
3256 1397 608
XhoI C'TCGA_G
Cuts at: 1189 1189
Size: 5261
XmaI C'CCGG_G
Cuts at: 590 2281 590
Size: 1691 3570
XmnI GAAnn'nnTTC
Cuts at: 223 223
Size: 5261
ZraI GAC'GTC
Cuts at: 1302 1302
Size: 5261
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NsiI PacI PmlI PshAI PsiI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI