Restriction map for
pF1KA0148
(Circular) (Six-base) MAPSORT of: pF1KA0148.seq Check: 5447 from: 1 to: 5156
pF1KA0148 5156 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AatII G_ACGT'C
Cuts at: 1476 1987 1476
Size: 511 4645
AccI GT'mk_AC
Cuts at: 2194 2194
Size: 5156
Acc65I G'GTAC_C
Cuts at: 1740 2172 1740
Size: 432 4724
AclI AA'CG_TT
Cuts at: 190 2150 4996 190
Size: 1960 2846 350
Fragments arranged by size:
2846 1960 350
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 2981 3413 4160 4428 274
Size: 2707 432 747 268 1002
Fragments arranged by size:
2707 1002 747 432 268
AflII C'TTAA_G
Cuts at: 1845 1845
Size: 5156
AflIII A'CryG_T
Cuts at: 1902 2508 3612 4589 1902
Size: 606 1104 977 2469
Fragments arranged by size:
2469 1104 977 606
AgeI A'CCGG_T
Cuts at: 3544 3544
Size: 5156
AhdI GACnn_n'nnGTC
Cuts at: 357 357
Size: 5156
AlwNI CAG_nnn'CTG
Cuts at: 2461 4028 2461
Size: 1567 3589
ApaLI G'TGCA_C
Cuts at: 3926 3926
Size: 5156
ApoI r'AATT_y
Cuts at: 421 1377 2160 4509 4765 421
Size: 956 783 2349 256 812
Fragments arranged by size:
2349 956 812 783 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5156
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5156
AvaI C'yCGr_G
Cuts at: 1200 1484 2176 1200
Size: 284 692 4180
Fragments arranged by size:
4180 692 284
BamHI G'GATC_C
Cuts at: 159 2181 159
Size: 2022 3134
BanI G'GyrC_C
Cuts at: 233 740 1740 2172 2586 2834 2869 233
Size: 507 1000 432 414 248 35 2520
Fragments arranged by size:
2520 1000 507 432 414 248 35
BanII G_rGCy'C
Cuts at: 1226 1844 2170 3200 1226
Size: 618 326 1030 3182
Fragments arranged by size:
3182 1030 618 326
BbeI G_GCGC'C
Cuts at: 237 2590 2838 237
Size: 2353 248 2555
Fragments arranged by size:
2555 2353 248
BbsI GAAGACnn'nnnn_
Cuts at: 381 1316 1816 2026 381
Size: 935 500 210 3511
Fragments arranged by size:
3511 935 500 210
BciVI GTATCCnnnnn_n'
Cuts at: 1892 3054 3815 5156 1892
Size: 1162 761 1341 1892
Fragments arranged by size:
1892 1341 1162 761
BclI T'GATC_A
Cuts at: 2676 2676
Size: 5156
BglII A'GATC_T
Cuts at: 501 2671 4708 501
Size: 2170 2037 949
Fragments arranged by size:
2170 2037 949
BlpI GC'TnA_GC
Cuts at: 877 2268 877
Size: 1391 3765
Bme1580I G_kGCm'C
Cuts at: 743 2781 2874 3930 743
Size: 2038 93 1056 1969
Fragments arranged by size:
2038 1969 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2368 2449 2784 4928 2368
Size: 81 335 2144 2596
Fragments arranged by size:
2596 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3523 3523
Size: 5156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1570 1636 1351
Size: 219 66 4871
Fragments arranged by size:
4871 219 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2332 3211 3703 4001 2332
Size: 879 492 298 3487
Fragments arranged by size:
3487 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 1507 4860 295
Size: 1212 3353 591
Fragments arranged by size:
3353 1212 591
BsaAI yAC'GTr
Cuts at: 1905 2509 1905
Size: 604 4552
BsaBI GATnn'nnATC
Cuts at: 6 2670 4707 6
Size: 2664 2037 455
Fragments arranged by size:
2664 2037 455
BsaHI Gr'CG_yC
Cuts at: 234 1169 1473 1984 2587 2835 3537 5033 234
Size: 935 304 511 603 248 702 1496 357
Fragments arranged by size:
1496 935 702 603 511 357 304 248
BsaWI w'CCGG_w
Cuts at: 122 1065 1516 2345 2866 3544 3818 3965 4376
Size: 943 451 829 521 678 274 147 411
Cuts at: 4376 122
Size: 902
Fragments arranged by size:
943 902 829 678 521 451 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1523 1553 1523
Size: 30 5126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1191 1191
Size: 5156
BseYI C'CCAG_C
Cuts at: 2581 3339 3916 4412 2581
Size: 758 577 496 3325
Fragments arranged by size:
3325 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1052 2698 2744 3952 104
Size: 948 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 948 46
BsiHKAI G_wGCw'C
Cuts at: 579 1226 1844 2170 2948 3138 3930 579
Size: 647 618 326 778 190 792 1805
Fragments arranged by size:
1805 792 778 647 618 326 190
BsiWI C'GTAC_G
Cuts at: 2090 2090
Size: 5156
BsmI GAATG_Cn'
Cuts at: 339 339
Size: 5156
BsmBI CGTCTCn'nnnn_
Cuts at: 985 1464 985
Size: 479 4677
Bsp1286I G_dGCh'C
Cuts at: 579 743 1226 1844 2170 2781 2874 2948 3138
Size: 164 483 618 326 611 93 74 190
Cuts at: 3138 3200 3930 579
Size: 62 730 1805
Fragments arranged by size:
1805 730 618 611 483 326 190 164
93 74 62
BspEI T'CCGG_A
Cuts at: 122 4376 122
Size: 4254 902
BspHI T'CATG_A
Cuts at: 4332 4332
Size: 5156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2064 2206 2420 2722 3103 2064
Size: 142 214 302 381 4117
Fragments arranged by size:
4117 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 3448 3502 4986 5151 50
Size: 3398 54 1484 165 55
Fragments arranged by size:
3398 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3068 3068
Size: 5156
BsrFI r'CCGG_y
Cuts at: 1346 3154 3335 3544 1346
Size: 1808 181 209 2958
Fragments arranged by size:
2958 1808 209 181
BssSI C'ACGA_G
Cuts at: 763 3427 3785 763
Size: 2664 358 2134
Fragments arranged by size:
2664 2134 358
BstAPI GCAn_nnn'nTGC
Cuts at: 345 907 2533 345
Size: 562 1626 2968
Fragments arranged by size:
2968 1626 562
BstBI TT'CG_AA
Cuts at: 1381 3517 1381
Size: 2136 3020
BstEII G'GTnAC_C
Cuts at: 1035 2121 1035
Size: 1086 4070
BstXI CCAn_nnnn'nTGG
Cuts at: 1668 2118 1668
Size: 450 4706
BstYI r'GATC_y
Cuts at: 1 159 501 2181 2648 2671 3006 3252 4264
Size: 158 342 1680 467 23 335 246 1012
Cuts at: 4264 4350 4362 4708 1
Size: 86 12 346 449
Fragments arranged by size:
1680 1012 467 449 346 342 335 246
158 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1387 1387
Size: 5156
BtgI C'CryG_G
Cuts at: 271 959 271
Size: 688 4468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 445 2082 3106 3287 3450 445
Size: 1637 1024 181 163 2151
Fragments arranged by size:
2151 1637 1024 181 163
BtsI GCAGTG_nn'
Cuts at: 219 283 1328 2399 219
Size: 64 1045 1071 2976
Fragments arranged by size:
2976 1071 1045 64
DraI TTT'AAA
Cuts at: 2155 2155
Size: 5156
DraIII CAC_nnn'GTG
Cuts at: 272 272
Size: 5156
DrdI GACnn_nn'nnGTC
Cuts at: 2862 3720 2862
Size: 858 4298
EaeI y'GGCC_r
Cuts at: 1723 2741 2915 3306 3333 4678 1723
Size: 1018 174 391 27 1345 2201
Fragments arranged by size:
2201 1345 1018 391 174 27
EagI C'GGCC_G
Cuts at: 2741 2741
Size: 5156
EarI CTCTTCn'nnn_
Cuts at: 1464 1562 3179 3389 1464
Size: 98 1617 210 3231
Fragments arranged by size:
3231 1617 210 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3364 3674 3820 4966 3364
Size: 310 146 1146 3554
Fragments arranged by size:
3554 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1224 1842 2168 1224
Size: 618 326 4212
Fragments arranged by size:
4212 618 326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1351 1570 1636 2981 3413 4160 4428 274
Size: 1077 219 66 1345 432 747 268 1002
Fragments arranged by size:
1345 1077 1002 747 432 268 219 66
EcoNI CCTnn'n_nnAGG
Cuts at: 602 602
Size: 5156
EcoO109I rG'GnC_Cy
Cuts at: 2034 2295 2034
Size: 261 4895
EcoRI G'AATT_C
Cuts at: 421 2160 421
Size: 1739 3417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3175 3175
Size: 5156
FspI TGC'GCA
Cuts at: 2937 2937
Size: 5156
HaeII r_GCGC'y
Cuts at: 237 762 1031 2590 2838 3860 4839 237
Size: 525 269 1559 248 1022 979 554
Fragments arranged by size:
1559 1022 979 554 525 269 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 1069 1101 1504 1536 2026 2058 2998
Size: 32 694 32 403 32 490 32 940
Cuts at: 2998 3028 3030 3060 343
Size: 30 2 30 2439
Fragments arranged by size:
2439 940 694 490 403 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 981 2195 981
Size: 1214 3942
HindIII A'AGCT_T
Cuts at: 1354 1354
Size: 5156
KasI G'GCGC_C
Cuts at: 233 2586 2834 233
Size: 2353 248 2555
Fragments arranged by size:
2555 2353 248
KpnI G_GTAC'C
Cuts at: 1744 2176 1744
Size: 432 4724
MluI A'CGCG_T
Cuts at: 4589 4589
Size: 5156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 304 1475 3827 4011 304
Size: 1171 2352 184 1449
Fragments arranged by size:
2352 1449 1171 184
MscI TGG'CCA
Cuts at: 1725 2917 1725
Size: 1192 3964
MslI CAynn'nnrTG
Cuts at: 833 964 1504 3145 3272 833
Size: 131 540 1641 127 2717
Fragments arranged by size:
2717 1641 540 131 127
MspA1I CmG'CkG
Cuts at: 152 2061 2118 2267 2581 2941 3954 4199 152
Size: 1909 57 149 314 360 1013 245 1109
Fragments arranged by size:
1909 1109 1013 360 314 245 149 57
NaeI GCC'GGC
Cuts at: 3337 3337
Size: 5156
NarI GG'CG_CC
Cuts at: 234 2587 2835 234
Size: 2353 248 2555
Fragments arranged by size:
2555 2353 248
NcoI C'CATG_G
Cuts at: 959 959
Size: 5156
NgoMIV G'CCGG_C
Cuts at: 3335 3335
Size: 5156
NspI r_CATG'y
Cuts at: 2209 2709 3616 2209
Size: 500 907 3749
Fragments arranged by size:
3749 907 500
PasI CC'CwG_GG
Cuts at: 2029 2029
Size: 5156
PciI A'CATG_T
Cuts at: 3612 3612
Size: 5156
PflMI CCAn_nnn'nTGG
Cuts at: 750 1732 750
Size: 982 4174
PmeI GTTT'AAAC
Cuts at: 2155 2155
Size: 5156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 4295
Size: 32 5124
PpuMI rG'GwC_Cy
Cuts at: 2034 2034
Size: 5156
PshAI GACnn'nnGTC
Cuts at: 1048 1476 2004 1048
Size: 428 528 4200
Fragments arranged by size:
4200 528 428
PsiI TTA'TAA
Cuts at: 850 850
Size: 5156
PstI C_TGCA'G
Cuts at: 151 214 2006 2203 2888 151
Size: 63 1792 197 685 2419
Fragments arranged by size:
2419 1792 685 197 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5156
PvuII CAG'CTG
Cuts at: 2061 2118 2581 2941 2061
Size: 57 463 360 4276
Fragments arranged by size:
4276 463 360 57
RsrII CG'GwC_CG
Cuts at: 3351 3351
Size: 5156
SacI G_AGCT'C
Cuts at: 1226 1844 2170 1226
Size: 618 326 4212
Fragments arranged by size:
4212 618 326
SalI G'TCGA_C
Cuts at: 2193 2193
Size: 5156
SanDI GG'GwC_CC
Cuts at: 2034 2034
Size: 5156
SapI GCTCTTCn'nnn_
Cuts at: 3179 3389 3179
Size: 210 4946
SbfI CC_TGCA'GG
Cuts at: 2203 2203
Size: 5156
ScaI AGT'ACT
Cuts at: 1081 4673 1081
Size: 3592 1564
SfcI C'TryA_G
Cuts at: 33 147 210 2002 2199 2884 3877 4068 33
Size: 114 63 1792 197 685 993 191 1121
Fragments arranged by size:
1792 1121 993 685 197 191 114 63
SfoI GGC'GCC
Cuts at: 235 2588 2836 235
Size: 2353 248 2555
Fragments arranged by size:
2555 2353 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5156
SmaI CCC'GGG
Cuts at: 1486 2178 1486
Size: 692 4464
SmlI C'TyrA_G
Cuts at: 1845 2311 3226 3718 3980 1845
Size: 466 915 492 262 3021
Fragments arranged by size:
3021 915 492 466 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5156
SphI G_CATG'C
Cuts at: 2209 2709 2209
Size: 500 4656
StyI C'CwwG_G
Cuts at: 959 2102 2290 2644 959
Size: 1143 188 354 3471
Fragments arranged by size:
3471 1143 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2061 2739 2872 3331 3540 2061
Size: 678 133 459 209 3677
Fragments arranged by size:
3677 678 459 209 133
TatI w'GTAC_w
Cuts at: 402 1079 1436 4671 402
Size: 677 357 3235 887
Fragments arranged by size:
3235 887 677 357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4673 4705 4673
Size: 32 5124
Tth111I GACn'n_nGTC
Cuts at: 2953 2953
Size: 5156
XbaI T'CTAG_A
Cuts at: 2187 2187
Size: 5156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1953 2651 1953
Size: 698 4458
XmaI C'CCGG_G
Cuts at: 1484 2176 1484
Size: 692 4464
ZraI GAC'GTC
Cuts at: 1474 1985 1474
Size: 511 4645
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI