Restriction map for
pF1KA0136
(Circular) (Six-base) MAPSORT of: pF1KA0136.seq Check: 5098 from: 1 to: 5930
pF1KA0136 5930 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 2968 2968
Size: 5930
Acc65I G'GTAC_C
Cuts at: 2946 2946
Size: 5930
AclI AA'CG_TT
Cuts at: 5770 5770
Size: 5930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1484 2054 2150 2696 2827 3755 4187 4540
Size: 1139 570 96 546 131 928 432 353
Cuts at: 4540 5202 345
Size: 662 1073
Fragments arranged by size:
1139 1073 928 662 570 546 432 353
131 96
AflII C'TTAA_G
Cuts at: 1162 1162
Size: 5930
AflIII A'CryG_T
Cuts at: 2481 3282 5082 5363 2481
Size: 801 1800 281 3048
Fragments arranged by size:
3048 1800 801 281
AlwNI CAG_nnn'CTG
Cuts at: 208 3235 4673 208
Size: 3027 1438 1465
Fragments arranged by size:
3027 1465 1438
ApaLI G'TGCA_C
Cuts at: 4768 4768
Size: 5930
ApoI r'AATT_y
Cuts at: 171 767 985 1293 1525 1659 1800 2934 5283
Size: 596 218 308 232 134 141 1134 2349
Cuts at: 5283 5539 171
Size: 256 562
Fragments arranged by size:
2349 1134 596 562 308 256 232 218
141 134
AseI AT'TA_AT
Cuts at: 20 214 578 1562 2384 20
Size: 194 364 984 822 3566
Fragments arranged by size:
3566 984 822 364 194
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5930
AvaI C'yCGr_G
Cuts at: 772 2950 772
Size: 2178 3752
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2021 2054 2021
Size: 33 5897
BamHI G'GATC_C
Cuts at: 2955 2955
Size: 5930
BanI G'GyrC_C
Cuts at: 2946 3360 3608 3643 2946
Size: 414 248 35 5233
Fragments arranged by size:
5233 414 248 35
BanII G_rGCy'C
Cuts at: 2944 3974 2944
Size: 1030 4900
BbeI G_GCGC'C
Cuts at: 3364 3612 3364
Size: 248 5682
BbsI GAAGACnn'nnnn_
Cuts at: 934 1644 2081 934
Size: 710 437 4783
Fragments arranged by size:
4783 710 437
BbvCI CC'TCA_GC
Cuts at: 139 139
Size: 5930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2152 2186 2152
Size: 34 5896
BciVI GTATCCnnnnn_n'
Cuts at: 125 2291 3828 4884 5930 125
Size: 2166 1537 1056 1046 125
Fragments arranged by size:
2166 1537 1056 1046 125
BclI T'GATC_A
Cuts at: 1326 2271 3450 1326
Size: 945 1179 3806
Fragments arranged by size:
3806 1179 945
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5930
BglII A'GATC_T
Cuts at: 3445 5482 3445
Size: 2037 3893
BlpI GC'TnA_GC
Cuts at: 349 3042 349
Size: 2693 3237
Bme1580I G_kGCm'C
Cuts at: 3555 3648 4772 3555
Size: 93 1124 4713
Fragments arranged by size:
4713 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 388 3142 3223 3558 5702 388
Size: 2754 81 335 2144 616
Fragments arranged by size:
2754 2144 616 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2791 4297 2791
Size: 1506 4424
Bpu10I CC'TnA_GC
Cuts at: 139 139
Size: 5930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3106 3985 4699 4997 3106
Size: 879 714 298 4039
Fragments arranged by size:
4039 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5634 5634
Size: 5930
BsaAI yAC'GTr
Cuts at: 913 3283 913
Size: 2370 3560
BsaBI GATnn'nnATC
Cuts at: 6 974 2837 3444 5481 6
Size: 968 1863 607 2037 455
Fragments arranged by size:
2037 1863 968 607 455
BsaHI Gr'CG_yC
Cuts at: 3361 3609 4311 5807 3361
Size: 248 702 1496 3484
Fragments arranged by size:
3484 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1542 3119 3640 4318 4729 4876 5150 1542
Size: 1577 521 678 411 147 274 2322
Fragments arranged by size:
2322 1577 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1770 1800 1770
Size: 30 5900
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2758 2758
Size: 5930
BseYI C'CCAG_C
Cuts at: 3355 4113 4778 5186 3355
Size: 758 665 408 4099
Fragments arranged by size:
4099 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 2569 221
Size: 2348 3582
BsiEI CG_ry'CG
Cuts at: 104 3472 3518 4748 104
Size: 3368 46 1230 1286
Fragments arranged by size:
3368 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2944 3722 3912 4772 2944
Size: 778 190 860 4102
Fragments arranged by size:
4102 860 778 190
BsmI GAATG_Cn'
Cuts at: 553 902 1770 2500 553
Size: 349 868 730 3983
Fragments arranged by size:
3983 868 730 349
Bsp1286I G_dGCh'C
Cuts at: 2944 3555 3648 3722 3912 3974 4772 2944
Size: 611 93 74 190 62 798 4102
Fragments arranged by size:
4102 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1881 4362 1881
Size: 2481 3449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1878 2980 3194 3496 3877 1878
Size: 1102 214 302 381 3931
Fragments arranged by size:
3931 1102 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4222 4276 5760 5925 50
Size: 4172 54 1484 165 55
Fragments arranged by size:
4172 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3842 3842
Size: 5930
BsrFI r'CCGG_y
Cuts at: 3928 4109 3928
Size: 181 5749
BssHII G'CGCG_C
Cuts at: 143 143
Size: 5930
BssSI C'ACGA_G
Cuts at: 4201 4909 4201
Size: 708 5222
BstAPI GCAn_nnn'nTGC
Cuts at: 2179 3307 2179
Size: 1128 4802
BstBI TT'CG_AA
Cuts at: 943 4291 943
Size: 3348 2582
BstYI r'GATC_y
Cuts at: 1 2955 3422 3445 3780 4026 4332 4344 4430
Size: 2954 467 23 335 246 306 12 86
Cuts at: 4430 5482 1
Size: 1052 449
Fragments arranged by size:
2954 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 859 1619 859
Size: 760 5170
BtgI C'CryG_G
Cuts at: 107 126 635 107
Size: 19 509 5402
Fragments arranged by size:
5402 509 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3880 4061 4224 3880
Size: 181 163 5586
Fragments arranged by size:
5586 181 163
BtsI GCAGTG_nn'
Cuts at: 2108 3173 2108
Size: 1065 4865
DraI TTT'AAA
Cuts at: 2929 2929
Size: 5930
DraIII CAC_nnn'GTG
Cuts at: 2659 2659
Size: 5930
DrdI GACnn_nn'nnGTC
Cuts at: 3636 4980 3636
Size: 1344 4586
EaeI y'GGCC_r
Cuts at: 190 1582 1965 3515 3689 4080 4107 5452 190
Size: 1392 383 1550 174 391 27 1345 668
Fragments arranged by size:
1550 1392 1345 668 391 383 174 27
EagI C'GGCC_G
Cuts at: 3515 3515
Size: 5930
EarI CTCTTCn'nnn_
Cuts at: 940 2795 3953 4163 940
Size: 1855 1158 210 2707
Fragments arranged by size:
2707 1855 1158 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1380 4138 4880 5026 5740 1380
Size: 2758 742 146 714 1570
Fragments arranged by size:
2758 1570 742 714 146
EcoICRI GAG'CTC
Cuts at: 2942 2942
Size: 5930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1484 2054 2150 2696 2827 3755 4187 4540
Size: 1139 570 96 546 131 928 432 353
Cuts at: 4540 5202 345
Size: 662 1073
Fragments arranged by size:
1139 1073 928 662 570 546 432 353
131 96
EcoO109I rG'GnC_Cy
Cuts at: 3069 3069
Size: 5930
EcoRI G'AATT_C
Cuts at: 767 2934 767
Size: 2167 3763
EcoRV GAT'ATC
Cuts at: 762 762
Size: 5930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 619 1628 3949 619
Size: 1009 2321 2600
Fragments arranged by size:
2600 2321 1009
FspI TGC'GCA
Cuts at: 3711 3711
Size: 5930
HaeII r_GCGC'y
Cuts at: 3364 3612 4842 5613 3364
Size: 248 1230 771 3681
Fragments arranged by size:
3681 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1882 1914 3772 3802 3804 3834 1882
Size: 32 1858 30 2 30 3978
Fragments arranged by size:
3978 1858 32 30 30 2
HincII GTy'rAC
Cuts at: 2799 2969 2799
Size: 170 5760
HindIII A'AGCT_T
Cuts at: 351 1082 1605 2432 351
Size: 731 523 827 3849
Fragments arranged by size:
3849 827 731 523
HpaI GTT'AAC
Cuts at: 2799 2799
Size: 5930
KasI G'GCGC_C
Cuts at: 3360 3608 3360
Size: 248 5682
KpnI G_GTAC'C
Cuts at: 2950 2950
Size: 5930
MluI A'CGCG_T
Cuts at: 5363 5363
Size: 5930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 378 1077 1110 1119 4689 4873 378
Size: 699 33 9 3570 184 1435
Fragments arranged by size:
3570 1435 699 184 33 9
MscI TGG'CCA
Cuts at: 192 1967 3691 192
Size: 1775 1724 2431
Fragments arranged by size:
2431 1775 1724
MslI CAynn'nnrTG
Cuts at: 484 1998 2762 3919 4046 484
Size: 1514 764 1157 127 2368
Fragments arranged by size:
2368 1514 1157 764 127
MspA1I CmG'CkG
Cuts at: 128 936 3041 3355 3715 4499 4744 128
Size: 808 2105 314 360 784 245 1314
Fragments arranged by size:
2105 1314 808 784 360 314 245
NaeI GCC'GGC
Cuts at: 4111 4111
Size: 5930
NarI GG'CG_CC
Cuts at: 3361 3609 3361
Size: 248 5682
NcoI C'CATG_G
Cuts at: 107 107
Size: 5930
NdeI CA'TA_TG
Cuts at: 2261 2411 2759 2261
Size: 150 348 5432
Fragments arranged by size:
5432 348 150
NgoMIV G'CCGG_C
Cuts at: 4109 4109
Size: 5930
NspI r_CATG'y
Cuts at: 535 2485 2983 3483 5086 535
Size: 1950 498 500 1603 1379
Fragments arranged by size:
1950 1603 1379 500 498
PciI A'CATG_T
Cuts at: 2481 5082 2481
Size: 2601 3329
PmeI GTTT'AAAC
Cuts at: 2929 2929
Size: 5930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 4376
Size: 32 5898
PsiI TTA'TAA
Cuts at: 1811 2246 1811
Size: 435 5495
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 632 664 632
Size: 32 5898
PstI C_TGCA'G
Cuts at: 1030 1875 2022 2175 2977 3662 1030
Size: 845 147 153 802 685 3298
Fragments arranged by size:
3298 845 802 685 153 147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5930
PvuII CAG'CTG
Cuts at: 936 3355 3715 936
Size: 2419 360 3151
Fragments arranged by size:
3151 2419 360
RsrII CG'GwC_CG
Cuts at: 4125 4125
Size: 5930
SacI G_AGCT'C
Cuts at: 2944 2944
Size: 5930
SacII CC_GC'GG
Cuts at: 129 129
Size: 5930
SalI G'TCGA_C
Cuts at: 2967 2967
Size: 5930
SapI GCTCTTCn'nnn_
Cuts at: 3953 4163 3953
Size: 210 5720
SbfI CC_TGCA'GG
Cuts at: 2977 2977
Size: 5930
ScaI AGT'ACT
Cuts at: 2922 5447 2922
Size: 2525 3405
SfcI C'TryA_G
Cuts at: 33 1026 1871 2018 2171 2973 3658 4626 4817
Size: 993 845 147 153 802 685 968 191
Cuts at: 4817 33
Size: 1146
Fragments arranged by size:
1146 993 968 845 802 685 191 153
147
SfoI GGC'GCC
Cuts at: 3362 3610 3362
Size: 248 5682
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5930
SmaI CCC'GGG
Cuts at: 2952 2952
Size: 5930
SmlI C'TyrA_G
Cuts at: 1162 3085 4000 4714 4976 1162
Size: 1923 915 714 262 2116
Fragments arranged by size:
2116 1923 915 714 262
SpeI A'CTAG_T
Cuts at: 67 178 67
Size: 111 5819
SphI G_CATG'C
Cuts at: 2983 3483 2983
Size: 500 5430
StyI C'CwwG_G
Cuts at: 107 3064 3418 107
Size: 2957 354 2619
Fragments arranged by size:
2957 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1114 1936 3513 3646 4105 4314 1114
Size: 822 1577 133 459 209 2730
Fragments arranged by size:
2730 1577 822 459 209 133
TatI w'GTAC_w
Cuts at: 1205 2920 5445 1205
Size: 1715 2525 1690
Fragments arranged by size:
2525 1715 1690
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 908 940 5447 5479 908
Size: 32 4507 32 1359
Fragments arranged by size:
4507 1359 32 32
Tth111I GACn'n_nGTC
Cuts at: 370 2255 3727 370
Size: 1885 1472 2573
Fragments arranged by size:
2573 1885 1472
XbaI T'CTAG_A
Cuts at: 2961 2961
Size: 5930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3425 3425
Size: 5930
XmaI C'CCGG_G
Cuts at: 2950 2950
Size: 5930
XmnI GAAnn'nnTTC
Cuts at: 415 1628 1659 415
Size: 1213 31 4686
Fragments arranged by size:
4686 1213 31
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PsiI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BfrBI BmgBI BmtI BpmI BsiWI
BsmBI BspEI BsrGI BstEII BstXI BstZ17I ClaI CspCI
EcoNI FseI FspAI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI