Restriction map for
pF1KA0131
(Circular) (Six-base) MAPSORT of: pF1KA0131.seq Check: 2297 from: 1 to: 5951
pF1KA0131 5951 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 942 2523 942
Size: 1581 4370
AatII G_ACGT'C
Cuts at: 902 902
Size: 5951
AccI GT'mk_AC
Cuts at: 2222 2989 2222
Size: 767 5184
Acc65I G'GTAC_C
Cuts at: 2967 2967
Size: 5951
AclI AA'CG_TT
Cuts at: 5791 5791
Size: 5951
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 1495 1870 3776 4208 4955 5223 1276
Size: 219 375 1906 432 747 268 2004
Fragments arranged by size:
2004 1906 747 432 375 268 219
AfeI AGC'GCT
Cuts at: 325 325
Size: 5951
AflIII A'CryG_T
Cuts at: 2697 2943 3303 4407 5384 2697
Size: 246 360 1104 977 3264
Fragments arranged by size:
3264 1104 977 360 246
AgeI A'CCGG_T
Cuts at: 2103 4339 2103
Size: 2236 3715
AlwNI CAG_nnn'CTG
Cuts at: 252 501 564 1011 1275 2400 3256 4823 252
Size: 249 63 447 264 1125 856 1567 1380
Fragments arranged by size:
1567 1380 1125 856 447 264 249 63
ApaI G_GGCC'C
Cuts at: 488 743 2093 2819 2881 2889 488
Size: 255 1350 726 62 8 3550
Fragments arranged by size:
3550 1350 726 255 62 8
ApaLI G'TGCA_C
Cuts at: 822 4721 822
Size: 3899 2052
ApoI r'AATT_y
Cuts at: 1567 2955 5304 5560 1567
Size: 1388 2349 256 1958
Fragments arranged by size:
2349 1958 1388 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5951
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5951
AvaI C'yCGr_G
Cuts at: 299 1072 2445 2581 2873 2971 299
Size: 773 1373 136 292 98 3279
Fragments arranged by size:
3279 1373 773 292 136 98
BamHI G'GATC_C
Cuts at: 1044 2976 1044
Size: 1932 4019
BanI G'GyrC_C
Cuts at: 269 527 724 1744 2106 2238 2967 3381 3629
Size: 258 197 1020 362 132 729 414 248
Cuts at: 3629 3664 269
Size: 35 2556
Fragments arranged by size:
2556 1020 729 414 362 258 248 197
132 35
BanII G_rGCy'C
Cuts at: 488 578 599 743 757 1194 1654 1877 2093
Size: 90 21 144 14 437 460 223 216
Cuts at: 2093 2753 2819 2881 2889 2965 3995 488
Size: 660 66 62 8 76 1030 2444
Fragments arranged by size:
2444 1030 660 460 437 223 216 144
90 76 66 62 21 14 8
BbeI G_GCGC'C
Cuts at: 273 531 3385 3633 273
Size: 258 2854 248 2591
Fragments arranged by size:
2854 2591 258 248
BbsI GAAGACnn'nnnn_
Cuts at: 619 1083 2181 619
Size: 464 1098 4389
Fragments arranged by size:
4389 1098 464
BbvCI CC'TCA_GC
Cuts at: 278 2901 278
Size: 2623 3328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1063 1097 2164 2198 1063
Size: 34 1067 34 4816
Fragments arranged by size:
4816 1067 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3849 4610 5951 3849
Size: 761 1341 3849
Fragments arranged by size:
3849 1341 761
BclI T'GATC_A
Cuts at: 3471 3471
Size: 5951
BglI GCCn_nnn'nGGC
Cuts at: 341 733 2115 2852 341
Size: 392 1382 737 3440
Fragments arranged by size:
3440 1382 737 392
BglII A'GATC_T
Cuts at: 1148 3466 5503 1148
Size: 2318 2037 1596
Fragments arranged by size:
2318 2037 1596
BlpI GC'TnA_GC
Cuts at: 190 850 2401 3063 190
Size: 660 1551 662 3078
Fragments arranged by size:
3078 1551 662 660
Bme1580I G_kGCm'C
Cuts at: 488 743 826 1670 1749 2093 2111 2819 2881
Size: 255 83 844 79 344 18 708 62
Cuts at: 2881 2889 3576 3669 4725 488
Size: 8 687 93 1056 1714
Fragments arranged by size:
1714 1056 844 708 687 344 255 93
83 79 62 18 8
BmgBI CAC'GTC
Cuts at: 522 522
Size: 5951
BmrI ACTGGGnnnn_n'
Cuts at: 1545 1596 2031 2801 3163 3244 3579 5723 1545
Size: 51 435 770 362 81 335 2144 1773
Fragments arranged by size:
2144 1773 770 435 362 335 81 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 345 606 2460 4318 345
Size: 261 1854 1858 1978
Fragments arranged by size:
1978 1858 1854 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 315 574 583 601 1034 1300 1423 1891
Size: 83 259 9 18 433 266 123 468
Cuts at: 1891 1948 2296 2362 232
Size: 57 348 66 3821
Fragments arranged by size:
3821 468 433 348 266 259 123 83
66 57 18 9
Bpu10I CC'TnA_GC
Cuts at: 278 1653 2901 278
Size: 1375 1248 3328
Fragments arranged by size:
3328 1375 1248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 802 1333 3127 4006 4498 4796 745
Size: 57 531 1794 879 492 298 1900
Fragments arranged by size:
1900 1794 879 531 492 298 57
BsaI GGTCTCn'nnnn_
Cuts at: 594 1405 1764 5655 594
Size: 811 359 3891 890
Fragments arranged by size:
3891 890 811 359
BsaAI yAC'GTr
Cuts at: 1956 2700 3304 1956
Size: 744 604 4603
Fragments arranged by size:
4603 744 604
BsaBI GATnn'nnATC
Cuts at: 6 3465 5502 6
Size: 3459 2037 455
Fragments arranged by size:
3459 2037 455
BsaHI Gr'CG_yC
Cuts at: 270 528 899 2267 3382 3630 4332 5828 270
Size: 258 371 1368 1115 248 702 1496 393
Fragments arranged by size:
1496 1368 1115 702 393 371 258 248
BsaWI w'CCGG_w
Cuts at: 377 959 1862 2103 3140 3661 4339 4613 4760
Size: 582 903 241 1037 521 678 274 147
Cuts at: 4760 5171 377
Size: 411 1157
Fragments arranged by size:
1157 1037 903 678 582 521 411 274
241 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2787 2817 2787
Size: 30 5921
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 697 1252 1543 697
Size: 555 291 5105
Fragments arranged by size:
5105 555 291
BseYI C'CCAG_C
Cuts at: 413 494 1594 1748 2113 2270 2679 2839 2889
Size: 81 1100 154 365 157 409 160 50
Cuts at: 2889 3376 4134 4711 5207 413
Size: 487 758 577 496 1157
Fragments arranged by size:
1157 1100 758 577 496 487 409 365
160 157 154 81 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1797 2179 2194 2416 1024
Size: 773 382 15 222 4559
Fragments arranged by size:
4559 773 382 222 15
BsiEI CG_ry'CG
Cuts at: 104 974 2184 3493 3539 4747 104
Size: 870 1210 1309 46 1208 1308
Fragments arranged by size:
1309 1308 1210 1208 870 46
BsiHKAI G_wGCw'C
Cuts at: 368 578 599 757 812 826 959 1654 1955
Size: 210 21 158 55 14 133 695 301
Cuts at: 1955 2471 2753 2965 3743 3933 4725 368
Size: 516 282 212 778 190 792 1594
Fragments arranged by size:
1594 792 778 695 516 301 282 212
210 190 158 133 55 21 14
BsmI GAATG_Cn'
Cuts at: 651 1688 651
Size: 1037 4914
BsmBI CGTCTCn'nnnn_
Cuts at: 154 154
Size: 5951
Bsp1286I G_dGCh'C
Cuts at: 368 488 578 599 743 757 812 826 959
Size: 120 90 21 144 14 55 14 133
Cuts at: 959 1194 1654 1670 1749 1877 1955 2093 2111
Size: 235 460 16 79 128 78 138 18
Cuts at: 2111 2471 2753 2819 2881 2889 2965 3576 3669
Size: 360 282 66 62 8 76 611 93
Cuts at: 3669 3743 3933 3995 4725 368
Size: 74 190 62 730 1594
Fragments arranged by size:
1594 730 611 460 360 282 235 190
144 138 133 128 120 93 90 79
78 76 74 66 62 62 55 21
18 16 14 14 8
BspEI T'CCGG_A
Cuts at: 377 959 1862 5171 377
Size: 582 903 3309 1157
Fragments arranged by size:
3309 1157 903 582
BspHI T'CATG_A
Cuts at: 5127 5127
Size: 5951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 854 899 942 2523 3001 3215 3517 3898 854
Size: 45 43 1581 478 214 302 381 2907
Fragments arranged by size:
2907 1581 478 381 302 214 45 43
BsrBI CCG'CTC
Cuts at: 50 115 138 675 4243 4297 5781 5946 50
Size: 65 23 537 3568 54 1484 165 55
Fragments arranged by size:
3568 1484 537 165 65 55 54 23
BsrDI GCAATG_nn'
Cuts at: 509 3863 509
Size: 3354 2597
BsrFI r'CCGG_y
Cuts at: 1376 1986 2103 2612 3949 4130 4339 1376
Size: 610 117 509 1337 181 209 2988
Fragments arranged by size:
2988 1337 610 509 209 181 117
BssHII G'CGCG_C
Cuts at: 832 832
Size: 5951
BssSI C'ACGA_G
Cuts at: 1489 2434 4222 4580 1489
Size: 945 1788 358 2860
Fragments arranged by size:
2860 1788 945 358
BstAPI GCAn_nnn'nTGC
Cuts at: 564 1275 3328 564
Size: 711 2053 3187
Fragments arranged by size:
3187 2053 711
BstBI TT'CG_AA
Cuts at: 4312 4312
Size: 5951
BstXI CCAn_nnnn'nTGG
Cuts at: 804 2259 2782 804
Size: 1455 523 3973
Fragments arranged by size:
3973 1455 523
BstYI r'GATC_y
Cuts at: 1 549 1044 1148 1766 2976 3443 3466 3801
Size: 548 495 104 618 1210 467 23 335
Cuts at: 3801 4047 5059 5145 5157 5503 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1210 1012 618 548 495 467 449 346
335 246 104 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2636 2636
Size: 5951
BtgI C'CryG_G
Cuts at: 107 336 2642 107
Size: 229 2306 3416
Fragments arranged by size:
3416 2306 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2060 3901 4082 4245 2060
Size: 1841 181 163 3766
Fragments arranged by size:
3766 1841 181 163
BtsI GCAGTG_nn'
Cuts at: 408 1264 1814 3194 408
Size: 856 550 1380 3165
Fragments arranged by size:
3165 1380 856 550
DraI TTT'AAA
Cuts at: 2950 2950
Size: 5951
DrdI GACnn_nn'nnGTC
Cuts at: 3657 4515 3657
Size: 858 5093
EaeI y'GGCC_r
Cuts at: 110 317 479 605 971 1398 1838 2075 2614
Size: 207 162 126 366 427 440 237 539
Cuts at: 2614 3536 3710 4101 4128 5473 110
Size: 922 174 391 27 1345 588
Fragments arranged by size:
1345 922 588 539 440 427 391 366
237 207 174 162 126 27
EagI C'GGCC_G
Cuts at: 971 3536 971
Size: 2565 3386
EarI CTCTTCn'nnn_
Cuts at: 535 1570 1888 2017 3974 4184 535
Size: 1035 318 129 1957 210 2302
Fragments arranged by size:
2302 1957 1035 318 210 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2238 2305 2761 4159 4469 4615 5761 2238
Size: 67 456 1398 310 146 1146 2428
Fragments arranged by size:
2428 1398 1146 456 310 146 67
EcoICRI GAG'CTC
Cuts at: 576 597 755 1652 2751 2963 576
Size: 21 158 897 1099 212 3564
Fragments arranged by size:
3564 1099 897 212 158 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 315 574 583 601 1034 1276 1300 1423
Size: 83 259 9 18 433 242 24 123
Cuts at: 1423 1495 1870 1891 1948 2296 2362 3776 4208
Size: 72 375 21 57 348 66 1414 432
Cuts at: 4208 4955 5223 232
Size: 747 268 960
Fragments arranged by size:
1414 960 747 433 432 375 348 268
259 242 123 83 72 66 57 24
21 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 469 763 949 1279 469
Size: 294 186 330 5141
Fragments arranged by size:
5141 330 294 186
EcoO109I rG'GnC_Cy
Cuts at: 303 485 739 740 1008 1038 1272 1506 1794
Size: 182 254 1 268 30 234 234 288
Cuts at: 1794 2121 2441 2484 2586 2647 2778 2816 2832
Size: 327 320 43 102 61 131 38 16
Cuts at: 2832 2877 2878 2885 2886 2919 3090 303
Size: 45 1 7 1 33 171 3164
Fragments arranged by size:
3164 327 320 288 268 254 234 234
182 171 131 102 61 45 43 38
33 30 16 7 1 1 1
EcoRI G'AATT_C
Cuts at: 2955 2955
Size: 5951
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1875 2769 3970 1875
Size: 894 1201 3856
Fragments arranged by size:
3856 1201 894
FspI TGC'GCA
Cuts at: 3732 3732
Size: 5951
HaeII r_GCGC'y
Cuts at: 273 327 501 531 2134 3385 3633 4655 5634
Size: 54 174 30 1603 1251 248 1022 979
Cuts at: 5634 273
Size: 590
Fragments arranged by size:
1603 1251 1022 979 590 248 174 54
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 534 566 1448 1480 1816 1848 3793 3823 3825
Size: 32 882 32 336 32 1945 30 2
Cuts at: 3825 3855 534
Size: 30 2630
Fragments arranged by size:
2630 1945 882 336 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 867 2990 867
Size: 2123 3828
HindIII A'AGCT_T
Cuts at: 372 372
Size: 5951
KasI G'GCGC_C
Cuts at: 269 527 3381 3629 269
Size: 258 2854 248 2591
Fragments arranged by size:
2854 2591 258 248
KpnI G_GTAC'C
Cuts at: 2971 2971
Size: 5951
MluI A'CGCG_T
Cuts at: 5384 5384
Size: 5951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4622 4806 4622
Size: 184 5767
MscI TGG'CCA
Cuts at: 3712 3712
Size: 5951
MslI CAynn'nnrTG
Cuts at: 1124 3940 4067 1124
Size: 2816 127 3008
Fragments arranged by size:
3008 2816 127
MspA1I CmG'CkG
Cuts at: 189 201 564 721 976 1104 1939 2113 2154
Size: 12 363 157 255 128 835 174 41
Cuts at: 2154 2255 2274 3062 3376 3736 4749 4994 189
Size: 101 19 788 314 360 1013 245 1146
Fragments arranged by size:
1146 1013 835 788 363 360 314 255
245 174 157 128 101 41 19 12
NaeI GCC'GGC
Cuts at: 1378 4132 1378
Size: 2754 3197
NarI GG'CG_CC
Cuts at: 270 528 3382 3630 270
Size: 258 2854 248 2591
Fragments arranged by size:
2854 2591 258 248
NcoI C'CATG_G
Cuts at: 107 2642 107
Size: 2535 3416
NgoMIV G'CCGG_C
Cuts at: 1376 4130 1376
Size: 2754 3197
NspI r_CATG'y
Cuts at: 1084 2481 3004 3504 4411 1084
Size: 1397 523 500 907 2624
Fragments arranged by size:
2624 1397 907 523 500
PasI CC'CwG_GG
Cuts at: 945 2881 2916 945
Size: 1936 35 3980
Fragments arranged by size:
3980 1936 35
PciI A'CATG_T
Cuts at: 4407 4407
Size: 5951
PflMI CCAn_nnn'nTGG
Cuts at: 1827 1827
Size: 5951
PfoI T'CCnGG_A
Cuts at: 578 662 1439 578
Size: 84 777 5090
Fragments arranged by size:
5090 777 84
PmeI GTTT'AAAC
Cuts at: 2950 2950
Size: 5951
PmlI CAC'GTG
Cuts at: 1956 2700 1956
Size: 744 5207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2751 5090 5122 2719
Size: 32 2339 32 3548
Fragments arranged by size:
3548 2339 32 32
PpuMI rG'GwC_Cy
Cuts at: 1794 2121 2647 1794
Size: 327 526 5098
Fragments arranged by size:
5098 526 327
PshAI GACnn'nnGTC
Cuts at: 166 2767 166
Size: 2601 3350
PspOMI G'GGCC_C
Cuts at: 484 739 2089 2815 2877 2885 484
Size: 255 1350 726 62 8 3550
Fragments arranged by size:
3550 1350 726 255 62 8
PspXI vC'TCGA_Gb
Cuts at: 2445 2445
Size: 5951
PstI C_TGCA'G
Cuts at: 149 221 248 431 569 1271 1481 1502 1718
Size: 72 27 183 138 702 210 21 216
Cuts at: 1718 2138 2555 2562 2998 3683 149
Size: 420 417 7 436 685 2417
Fragments arranged by size:
2417 702 685 436 420 417 216 210
183 138 72 27 21 7
PvuI CG_AT'CG
Cuts at: 104 2184 104
Size: 2080 3871
PvuII CAG'CTG
Cuts at: 189 201 564 2154 2255 2274 3376 3736 189
Size: 12 363 1590 101 19 1102 360 2404
Fragments arranged by size:
2404 1590 1102 363 360 101 19 12
RsrII CG'GwC_CG
Cuts at: 4146 4146
Size: 5951
SacI G_AGCT'C
Cuts at: 578 599 757 1654 2753 2965 578
Size: 21 158 897 1099 212 3564
Fragments arranged by size:
3564 1099 897 212 158 21
SalI G'TCGA_C
Cuts at: 2988 2988
Size: 5951
SanDI GG'GwC_CC
Cuts at: 2647 2647
Size: 5951
SapI GCTCTTCn'nnn_
Cuts at: 535 1570 3974 4184 535
Size: 1035 2404 210 2302
Fragments arranged by size:
2404 2302 1035 210
SbfI CC_TGCA'GG
Cuts at: 2998 2998
Size: 5951
ScaI AGT'ACT
Cuts at: 5468 5468
Size: 5951
SfcI C'TryA_G
Cuts at: 33 145 217 244 427 565 1267 1477 1498
Size: 112 72 27 183 138 702 210 21
Cuts at: 1498 1714 2134 2551 2558 2994 3679 4672 4863
Size: 216 420 417 7 436 685 993 191
Cuts at: 4863 33
Size: 1121
Fragments arranged by size:
1121 993 702 685 436 420 417 216
210 191 183 138 112 72 27 21
7
SfoI GGC'GCC
Cuts at: 271 529 3383 3631 271
Size: 258 2854 248 2591
Fragments arranged by size:
2854 2591 258 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5951
SgrAI Cr'CCGG_yG
Cuts at: 1986 1986
Size: 5951
SmaI CCC'GGG
Cuts at: 301 2875 2973 301
Size: 2574 98 3279
Fragments arranged by size:
3279 2574 98
SmlI C'TyrA_G
Cuts at: 760 817 1072 1348 2445 3106 4021 4513 4775
Size: 57 255 276 1097 661 915 492 262
Cuts at: 4775 760
Size: 1936
Fragments arranged by size:
1936 1097 915 661 492 276 262 255
57
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5951
SphI G_CATG'C
Cuts at: 1084 2481 3004 3504 1084
Size: 1397 523 500 3531
Fragments arranged by size:
3531 1397 523 500
StyI C'CwwG_G
Cuts at: 107 1064 2642 2781 3085 3439 107
Size: 957 1578 139 304 354 2619
Fragments arranged by size:
2619 1578 957 354 304 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1003 1232 1429 3534 3667 4126 4335 1003
Size: 229 197 2105 133 459 209 2619
Fragments arranged by size:
2619 2105 459 229 209 197 133
TatI w'GTAC_w
Cuts at: 1553 2039 5466 1553
Size: 486 3427 2038
Fragments arranged by size:
3427 2038 486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5468 5500 5468
Size: 32 5919
Tth111I GACn'n_nGTC
Cuts at: 3748 3748
Size: 5951
XbaI T'CTAG_A
Cuts at: 2982 2982
Size: 5951
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1126 1618 2847 3446 1126
Size: 492 1229 599 3631
Fragments arranged by size:
3631 1229 599 492
XhoI C'TCGA_G
Cuts at: 1072 2445 1072
Size: 1373 4578
XmaI C'CCGG_G
Cuts at: 299 2873 2971 299
Size: 2574 98 3279
Fragments arranged by size:
3279 2574 98
ZraI GAC'GTC
Cuts at: 900 900
Size: 5951
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsrGI BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PsiI PsrI SacII SexAI
SfiI SnaBI SrfI SspI StuI SwaI XmnI