Restriction map for
pF1KA0121
(Circular) (Six-base) MAPSORT of: pF1KA0121.seq Check: 4041 from: 1 to: 3983
pF1KA0121 3983 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 2240 2593 3255 1808
Size: 432 353 662 2536
Fragments arranged by size:
2536 662 432 353
AfeI AGC'GCT
Cuts at: 708 708
Size: 3983
AflIII A'CryG_T
Cuts at: 848 1335 3135 3416 848
Size: 487 1800 281 1415
Fragments arranged by size:
1800 1415 487 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 577 609 577
Size: 32 3951
AlwNI CAG_nnn'CTG
Cuts at: 210 665 1288 2726 210
Size: 455 623 1438 1467
Fragments arranged by size:
1467 1438 623 455
ApaLI G'TGCA_C
Cuts at: 2821 2821
Size: 3983
ApoI r'AATT_y
Cuts at: 987 3336 3592 987
Size: 2349 256 1378
Fragments arranged by size:
2349 1378 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 398 567 676 778 1003 398
Size: 169 109 102 225 3378
Fragments arranged by size:
3378 225 169 109 102
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 619 786 999 1413 1661 1696 619
Size: 167 213 414 248 35 2906
Fragments arranged by size:
2906 414 248 213 167 35
BanII G_rGCy'C
Cuts at: 779 814 997 2027 779
Size: 35 183 1030 2735
Fragments arranged by size:
2735 1030 183 35
BbeI G_GCGC'C
Cuts at: 623 1417 1665 623
Size: 794 248 2941
Fragments arranged by size:
2941 794 248
BbvCI CC'TCA_GC
Cuts at: 229 229
Size: 3983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 402 436 811 845 402
Size: 34 375 34 3540
Fragments arranged by size:
3540 375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2937 3983 1881
Size: 1056 1046 1881
Fragments arranged by size:
1881 1056 1046
BclI T'GATC_A
Cuts at: 599 1503 599
Size: 904 3079
BglI GCCn_nnn'nGGC
Cuts at: 455 455
Size: 3983
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 1608 1701 2825 1608
Size: 93 1124 2766
Fragments arranged by size:
2766 1124 93
BmgBI CAC'GTC
Cuts at: 327 327
Size: 3983
BmrI ACTGGGnnnn_n'
Cuts at: 1195 1276 1611 3755 1195
Size: 81 335 2144 1423
Fragments arranged by size:
2144 1423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 921 2350 921
Size: 1429 2554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 841 514
Size: 327 3656
Bpu10I CC'TnA_GC
Cuts at: 229 229
Size: 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2038 2752 3050 1159
Size: 879 714 298 2092
Fragments arranged by size:
2092 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 386 978 3687 386
Size: 592 2709 682
Fragments arranged by size:
2709 682 592
BsaAI yAC'GTr
Cuts at: 851 1336 851
Size: 485 3498
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 115 530 620 1414 1662 2364 3860 115
Size: 415 90 794 248 702 1496 238
Fragments arranged by size:
1496 794 702 415 248 238 90
BsaWI w'CCGG_w
Cuts at: 692 1172 1693 2371 2782 2929 3203 692
Size: 480 521 678 411 147 274 1472
Fragments arranged by size:
1472 678 521 480 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 234 264 313 343 577 607 943 973 234
Size: 30 49 30 234 30 336 30 3244
Fragments arranged by size:
3244 336 234 49 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 751 751
Size: 3983
BseYI C'CCAG_C
Cuts at: 265 481 1408 2166 2831 3239 265
Size: 216 927 758 665 408 1009
Fragments arranged by size:
1009 927 758 665 408 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 436
Size: 3983
BsiEI CG_ry'CG
Cuts at: 104 1525 1571 2801 104
Size: 1421 46 1230 1286
Fragments arranged by size:
1421 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 997 1775 1965 2825 997
Size: 778 190 860 2155
Fragments arranged by size:
2155 860 778 190
BsmI GAATG_Cn'
Cuts at: 206 206
Size: 3983
BsmBI CGTCTCn'nnnn_
Cuts at: 106 333 106
Size: 227 3756
Bsp1286I G_dGCh'C
Cuts at: 779 814 997 1608 1701 1775 1965 2027 2825
Size: 35 183 611 93 74 190 62 798
Cuts at: 2825 779
Size: 1937
Fragments arranged by size:
1937 798 611 190 183 93 74 62
35
BspHI T'CATG_A
Cuts at: 2415 2415
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1247 1549 1930 1033
Size: 214 302 381 3086
Fragments arranged by size:
3086 381 302 214
BsrBI CCG'CTC
Cuts at: 50 405 576 2275 2329 3813 3978 50
Size: 355 171 1699 54 1484 165 55
Fragments arranged by size:
1699 1484 355 171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 213 244 544 587 783 1981 2162 213
Size: 31 300 43 196 1198 181 2034
Fragments arranged by size:
2034 1198 300 196 181 43 31
BsrGI T'GTAC_A
Cuts at: 467 467
Size: 3983
BssHII G'CGCG_C
Cuts at: 426 426
Size: 3983
BssSI C'ACGA_G
Cuts at: 2254 2962 2254
Size: 708 3275
BstAPI GCAn_nnn'nTGC
Cuts at: 210 1360 210
Size: 1150 2833
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstXI CCAn_nnnn'nTGG
Cuts at: 840 840
Size: 3983
BstYI r'GATC_y
Cuts at: 1 1008 1475 1498 1833 2079 2385 2397 2483
Size: 1007 467 23 335 246 306 12 86
Cuts at: 2483 3535 1
Size: 1052 449
Fragments arranged by size:
1052 1007 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 728 815 107
Size: 621 87 3275
Fragments arranged by size:
3275 621 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 636 1933 2114 2277 636
Size: 1297 181 163 2342
Fragments arranged by size:
2342 1297 181 163
BtsI GCAGTG_nn'
Cuts at: 642 1226 642
Size: 584 3399
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 3033 1689
Size: 1344 2639
EaeI y'GGCC_r
Cuts at: 869 1568 1742 2133 2160 3505 869
Size: 699 174 391 27 1345 1347
Fragments arranged by size:
1347 1345 699 391 174 27
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 891 2191 2933 3079 3793 891
Size: 1300 742 146 714 1081
Fragments arranged by size:
1300 1081 742 714 146
EcoICRI GAG'CTC
Cuts at: 995 995
Size: 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 841 1808 2240 2593 3255 514
Size: 327 967 432 353 662 1242
Fragments arranged by size:
1242 967 662 432 353 327
EcoNI CCTnn'n_nnAGG
Cuts at: 909 909
Size: 3983
EcoO109I rG'GnC_Cy
Cuts at: 303 1122 303
Size: 819 3164
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3983
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 623 710 1417 1665 2895 3666 623
Size: 87 707 248 1230 771 940
Fragments arranged by size:
1230 940 771 707 248 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 313 345 868 900 1825 1855 1857
Size: 32 166 32 523 32 925 30 2
Cuts at: 1857 1887 115
Size: 30 2211
Fragments arranged by size:
2211 925 523 166 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1022 1022
Size: 3983
KasI G'GCGC_C
Cuts at: 619 1413 1661 619
Size: 794 248 2941
Fragments arranged by size:
2941 794 248
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2742 2926 2742
Size: 184 3799
MscI TGG'CCA
Cuts at: 1744 1744
Size: 3983
MslI CAynn'nnrTG
Cuts at: 158 1972 2099 158
Size: 1814 127 2042
Fragments arranged by size:
2042 1814 127
MspA1I CmG'CkG
Cuts at: 662 1094 1408 1768 2552 2797 662
Size: 432 314 360 784 245 1848
Fragments arranged by size:
1848 784 432 360 314 245
NaeI GCC'GGC
Cuts at: 546 785 2164 546
Size: 239 1379 2365
Fragments arranged by size:
2365 1379 239
NarI GG'CG_CC
Cuts at: 620 1414 1662 620
Size: 794 248 2941
Fragments arranged by size:
2941 794 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 544 783 2162 544
Size: 239 1379 2365
Fragments arranged by size:
2365 1379 239
NspI r_CATG'y
Cuts at: 157 1036 1536 3139 157
Size: 879 500 1603 1001
Fragments arranged by size:
1603 1001 879 500
PciI A'CATG_T
Cuts at: 3135 3135
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PmlI CAC'GTG
Cuts at: 851 851
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 577 609 2429 2461 577
Size: 32 1820 32 2099
Fragments arranged by size:
2099 1820 32 32
PpuMI rG'GwC_Cy
Cuts at: 303 303
Size: 3983
PstI C_TGCA'G
Cuts at: 1030 1715 1030
Size: 685 3298
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 1408 1768 1408
Size: 360 3623
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 997 997
Size: 3983
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 1026 1711 2679 2870 33
Size: 993 685 968 191 1146
Fragments arranged by size:
1146 993 968 685 191
SfoI GGC'GCC
Cuts at: 621 1415 1663 621
Size: 794 248 2941
Fragments arranged by size:
2941 794 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 400 569 678 1005 400
Size: 169 109 327 3378
Fragments arranged by size:
3378 327 169 109
SmlI C'TyrA_G
Cuts at: 1138 2053 2767 3029 1138
Size: 915 714 262 2092
Fragments arranged by size:
2092 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 157 1036 1536 157
Size: 879 500 2604
Fragments arranged by size:
2604 879 500
SrfI GCCC'GGGC
Cuts at: 678 678
Size: 3983
StyI C'CwwG_G
Cuts at: 107 486 872 1117 1471 107
Size: 379 386 245 354 2619
Fragments arranged by size:
2619 386 379 354 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 804 832 1566 1699 2158 2367 804
Size: 28 734 133 459 209 2420
Fragments arranged by size:
2420 734 459 209 133 28
TatI w'GTAC_w
Cuts at: 467 3498 467
Size: 3031 952
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 234 266 943 975 3500 3532 234
Size: 32 677 32 2525 32 685
Fragments arranged by size:
2525 685 677 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 325 1780 325
Size: 1455 2528
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 522 1478 489
Size: 33 956 2994
Fragments arranged by size:
2994 956 33
XmaI C'CCGG_G
Cuts at: 398 567 676 1003 398
Size: 169 109 327 3378
Fragments arranged by size:
3378 327 169 109
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BfrBI BmtI BsiWI BspEI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SspI StuI SwaI XhoI XmnI ZraI