Restriction map for
pF1KA0113
(Circular) (Six-base) MAPSORT of: pF1KA0113.seq Check: 8801 from: 1 to: 5021
pF1KA0113 5021 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 596 1140 1491 596
Size: 544 351 4126
Fragments arranged by size:
4126 544 351
AccI GT'mk_AC
Cuts at: 1905 2059 1905
Size: 154 4867
Acc65I G'GTAC_C
Cuts at: 2037 2037
Size: 5021
AclI AA'CG_TT
Cuts at: 4861 4861
Size: 5021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 793 1597 2846 3278 4025 4293 232
Size: 561 804 1249 432 747 268 960
Fragments arranged by size:
1249 960 804 747 561 432 268
AflIII A'CryG_T
Cuts at: 2373 3477 4454 2373
Size: 1104 977 2940
Fragments arranged by size:
2940 1104 977
AgeI A'CCGG_T
Cuts at: 3409 3409
Size: 5021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 595 1060 595
Size: 465 4556
AlwNI CAG_nnn'CTG
Cuts at: 415 597 738 940 1131 1470 2326 3893 415
Size: 182 141 202 191 339 856 1567 1543
Fragments arranged by size:
1567 1543 856 339 202 191 182 141
ApaI G_GGCC'C
Cuts at: 553 1452 553
Size: 899 4122
ApaLI G'TGCA_C
Cuts at: 700 3791 700
Size: 3091 1930
ApoI r'AATT_y
Cuts at: 201 478 507 837 2025 4374 4630 201
Size: 277 29 330 1188 2349 256 592
Fragments arranged by size:
2349 1188 592 330 277 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 5021
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
AvaI C'yCGr_G
Cuts at: 2041 2041
Size: 5021
BamHI G'GATC_C
Cuts at: 2046 2046
Size: 5021
BanI G'GyrC_C
Cuts at: 466 1782 2037 2451 2699 2734 466
Size: 1316 255 414 248 35 2753
Fragments arranged by size:
2753 1316 414 255 248 35
BanII G_rGCy'C
Cuts at: 352 374 553 848 1318 1435 1452 1724 1942
Size: 22 179 295 470 117 17 272 218
Cuts at: 1942 2035 3065 352
Size: 93 1030 2308
Fragments arranged by size:
2308 1030 470 295 272 218 179 117
93 22 17
BbeI G_GCGC'C
Cuts at: 2455 2703 2455
Size: 248 4773
BciVI GTATCCnnnnn_n'
Cuts at: 2919 3680 5021 2919
Size: 761 1341 2919
Fragments arranged by size:
2919 1341 761
BclI T'GATC_A
Cuts at: 2541 2541
Size: 5021
BglII A'GATC_T
Cuts at: 1508 2536 4573 1508
Size: 1028 2037 1956
Fragments arranged by size:
2037 1956 1028
BlpI GC'TnA_GC
Cuts at: 368 1312 2133 368
Size: 944 821 3256
Fragments arranged by size:
3256 944 821
Bme1580I G_kGCm'C
Cuts at: 158 553 704 1452 2646 2739 3795 158
Size: 395 151 748 1194 93 1056 1384
Fragments arranged by size:
1384 1194 1056 748 395 151 93
BmrI ACTGGGnnnn_n'
Cuts at: 419 2233 2314 2649 4793 419
Size: 1814 81 335 2144 647
Fragments arranged by size:
2144 1814 647 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1684 3388 1684
Size: 1704 3317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 694 1045 1183 1396 619
Size: 75 351 138 213 4244
Fragments arranged by size:
4244 351 213 138 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 2197 3076 3568 3866 832
Size: 1365 879 492 298 1987
Fragments arranged by size:
1987 1365 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 595 1234 4725 595
Size: 639 3491 891
Fragments arranged by size:
3491 891 639
BsaAI yAC'GTr
Cuts at: 2374 2374
Size: 5021
BsaBI GATnn'nnATC
Cuts at: 6 2535 4572 6
Size: 2529 2037 455
Fragments arranged by size:
2529 2037 455
BsaHI Gr'CG_yC
Cuts at: 1902 2452 2700 3402 4898 1902
Size: 550 248 702 1496 2025
Fragments arranged by size:
2025 1496 702 550 248
BsaWI w'CCGG_w
Cuts at: 128 1079 2210 2731 3409 3683 3830 4241 128
Size: 951 1131 521 678 274 147 411 908
Fragments arranged by size:
1131 951 908 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 387 417 387
Size: 30 4991
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 462 514 640 1198 1252 1531 1576 1663
Size: 149 52 126 558 54 279 45 87
Cuts at: 1663 313
Size: 3671
Fragments arranged by size:
3671 558 279 149 126 87 54 52
45
BseYI C'CCAG_C
Cuts at: 546 1625 2446 3204 3781 4277 546
Size: 1079 821 758 577 496 1290
Fragments arranged by size:
1290 1079 821 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 568
Size: 5021
BsiEI CG_ry'CG
Cuts at: 104 2563 2609 3817 104
Size: 2459 46 1208 1308
Fragments arranged by size:
2459 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 374 704 848 1850 1942 2035 2813 3003 3795
Size: 330 144 1002 92 93 778 190 792
Cuts at: 3795 374
Size: 1600
Fragments arranged by size:
1600 1002 792 778 330 190 144 93
92
BsmI GAATG_Cn'
Cuts at: 1636 1636
Size: 5021
Bsp1286I G_dGCh'C
Cuts at: 158 352 374 553 704 848 1318 1435 1452
Size: 194 22 179 151 144 470 117 17
Cuts at: 1452 1724 1850 1942 2035 2646 2739 2813 3003
Size: 272 126 92 93 611 93 74 190
Cuts at: 3003 3065 3795 158
Size: 62 730 1384
Fragments arranged by size:
1384 730 611 470 272 194 190 179
151 144 126 117 93 93 92 74
62 22 17
BspEI T'CCGG_A
Cuts at: 4241 4241
Size: 5021
BspHI T'CATG_A
Cuts at: 4197 4197
Size: 5021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 596 958 1140 1307 1491 2071 2285 2587 2968
Size: 362 182 167 184 580 214 302 381
Cuts at: 2968 596
Size: 2649
Fragments arranged by size:
2649 580 381 362 302 214 184 182
167
BsrBI CCG'CTC
Cuts at: 50 1173 3313 3367 4851 5016 50
Size: 1123 2140 54 1484 165 55
Fragments arranged by size:
2140 1484 1123 165 55 54
BsrDI GCAATG_nn'
Cuts at: 873 2933 873
Size: 2060 2961
BsrFI r'CCGG_y
Cuts at: 3019 3200 3409 3019
Size: 181 209 4631
Fragments arranged by size:
4631 209 181
BssSI C'ACGA_G
Cuts at: 3292 3650 3292
Size: 358 4663
BstAPI GCAn_nnn'nTGC
Cuts at: 2398 2398
Size: 5021
BstBI TT'CG_AA
Cuts at: 1923 3382 1923
Size: 1459 3562
BstEII G'GTnAC_C
Cuts at: 1608 1608
Size: 5021
BstXI CCAn_nnnn'nTGG
Cuts at: 540 1842 540
Size: 1302 3719
BstYI r'GATC_y
Cuts at: 1 131 1352 1508 1814 2046 2513 2536 2871
Size: 130 1221 156 306 232 467 23 335
Cuts at: 2871 3117 4129 4215 4227 4573 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1221 1012 467 449 346 335 306 246
232 156 130 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 159 497 1456 1698 2012 159
Size: 338 959 242 314 3168
Fragments arranged by size:
3168 959 338 314 242
BtgI C'CryG_G
Cuts at: 107 636 1778 1791 1835 1841 107
Size: 529 1142 13 44 6 3287
Fragments arranged by size:
3287 1142 529 44 13 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 553 2971 3152 3315 553
Size: 2418 181 163 2259
Fragments arranged by size:
2418 2259 181 163
BtsI GCAGTG_nn'
Cuts at: 941 1044 1069 1545 2264 941
Size: 103 25 476 719 3698
Fragments arranged by size:
3698 719 476 103 25
DraI TTT'AAA
Cuts at: 2020 2020
Size: 5021
DrdI GACnn_nn'nnGTC
Cuts at: 391 2727 3585 391
Size: 2336 858 1827
Fragments arranged by size:
2336 1827 858
EaeI y'GGCC_r
Cuts at: 639 895 1214 1838 2606 2780 3171 3198 4543
Size: 256 319 624 768 174 391 27 1345
Cuts at: 4543 639
Size: 1117
Fragments arranged by size:
1345 1117 768 624 391 319 256 174
27
EagI C'GGCC_G
Cuts at: 2606 2606
Size: 5021
EarI CTCTTCn'nnn_
Cuts at: 251 652 1565 1675 1882 3044 3254 251
Size: 401 913 110 207 1162 210 2018
Fragments arranged by size:
2018 1162 913 401 210 207 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3229 3539 3685 4831 3229
Size: 310 146 1146 3419
Fragments arranged by size:
3419 1146 310 146
EcoICRI GAG'CTC
Cuts at: 372 846 1940 2033 372
Size: 474 1094 93 3360
Fragments arranged by size:
3360 1094 474 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 619 694 793 1045 1183 1396 1597 2846
Size: 387 75 99 252 138 213 201 1249
Cuts at: 2846 3278 4025 4293 232
Size: 432 747 268 960
Fragments arranged by size:
1249 960 747 432 387 268 252 213
201 138 99 75
EcoNI CCTnn'n_nnAGG
Cuts at: 193 193
Size: 5021
EcoO109I rG'GnC_Cy
Cuts at: 550 792 975 1371 1448 1449 1745 2008 2160
Size: 242 183 396 77 1 296 263 152
Cuts at: 2160 550
Size: 3411
Fragments arranged by size:
3411 396 296 263 242 183 152 77
1
EcoRI G'AATT_C
Cuts at: 201 507 2025 201
Size: 306 1518 3197
Fragments arranged by size:
3197 1518 306
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 5021
FspI TGC'GCA
Cuts at: 2802 2802
Size: 5021
HaeII r_GCGC'y
Cuts at: 189 547 597 2455 2703 3725 4704 189
Size: 358 50 1858 248 1022 979 506
Fragments arranged by size:
1858 1022 979 506 358 248 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 871 903 1792 1824 1941 1973 2863 2893 2895
Size: 32 889 32 117 32 890 30 2
Cuts at: 2895 2925 871
Size: 30 2967
Fragments arranged by size:
2967 890 889 117 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2060 2060
Size: 5021
KasI G'GCGC_C
Cuts at: 2451 2699 2451
Size: 248 4773
KpnI G_GTAC'C
Cuts at: 2041 2041
Size: 5021
MluI A'CGCG_T
Cuts at: 4454 4454
Size: 5021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 764 3692 3876 764
Size: 2928 184 1909
Fragments arranged by size:
2928 1909 184
MscI TGG'CCA
Cuts at: 1216 1840 2782 1216
Size: 624 942 3455
Fragments arranged by size:
3455 942 624
MslI CAynn'nnrTG
Cuts at: 533 707 1840 3010 3137 533
Size: 174 1133 1170 127 2417
Fragments arranged by size:
2417 1170 1133 174 127
MspA1I CmG'CkG
Cuts at: 804 1257 1311 1359 2132 2446 2806 3819 4064
Size: 453 54 48 773 314 360 1013 245
Cuts at: 4064 804
Size: 1761
Fragments arranged by size:
1761 1013 773 453 360 314 245 54
48
NaeI GCC'GGC
Cuts at: 3202 3202
Size: 5021
NarI GG'CG_CC
Cuts at: 2452 2700 2452
Size: 248 4773
NcoI C'CATG_G
Cuts at: 107 1778 1791 1835 1841 107
Size: 1671 13 44 6 3287
Fragments arranged by size:
3287 1671 44 13 6
NgoMIV G'CCGG_C
Cuts at: 3200 3200
Size: 5021
NspI r_CATG'y
Cuts at: 422 2074 2574 3481 422
Size: 1652 500 907 1962
Fragments arranged by size:
1962 1652 907 500
PasI CC'CwG_GG
Cuts at: 142 142
Size: 5021
PciI A'CATG_T
Cuts at: 3477 3477
Size: 5021
PflMI CCAn_nnn'nTGG
Cuts at: 463 1792 1950 463
Size: 1329 158 3534
Fragments arranged by size:
3534 1329 158
PmeI GTTT'AAAC
Cuts at: 2020 2020
Size: 5021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 4160
Size: 32 4989
PpuMI rG'GwC_Cy
Cuts at: 975 975
Size: 5021
PspOMI G'GGCC_C
Cuts at: 549 1448 549
Size: 899 4122
PstI C_TGCA'G
Cuts at: 593 734 1304 1601 2068 2753 593
Size: 141 570 297 467 685 2861
Fragments arranged by size:
2861 685 570 467 297 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5021
PvuII CAG'CTG
Cuts at: 1257 1311 2446 2806 1257
Size: 54 1135 360 3472
Fragments arranged by size:
3472 1135 360 54
RsrII CG'GwC_CG
Cuts at: 3216 3216
Size: 5021
SacI G_AGCT'C
Cuts at: 374 848 1942 2035 374
Size: 474 1094 93 3360
Fragments arranged by size:
3360 1094 474 93
SalI G'TCGA_C
Cuts at: 2058 2058
Size: 5021
SapI GCTCTTCn'nnn_
Cuts at: 652 1565 3044 3254 652
Size: 913 1479 210 2419
Fragments arranged by size:
2419 1479 913 210
SbfI CC_TGCA'GG
Cuts at: 1304 2068 1304
Size: 764 4257
ScaI AGT'ACT
Cuts at: 4538 4538
Size: 5021
SfcI C'TryA_G
Cuts at: 33 589 730 1300 1597 1973 2064 2749 3742
Size: 556 141 570 297 376 91 685 993
Cuts at: 3742 3933 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 570 556 376 297 191
141 91
SfoI GGC'GCC
Cuts at: 2453 2701 2453
Size: 248 4773
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
SmaI CCC'GGG
Cuts at: 2043 2043
Size: 5021
SmlI C'TyrA_G
Cuts at: 847 2176 3091 3583 3845 847
Size: 1329 915 492 262 2023
Fragments arranged by size:
2023 1329 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5021
SphI G_CATG'C
Cuts at: 422 2074 2574 422
Size: 1652 500 2869
Fragments arranged by size:
2869 1652 500
StuI AGG'CCT
Cuts at: 1696 1971 1696
Size: 275 4746
StyI C'CwwG_G
Cuts at: 107 344 695 992 1271 1778 1791 1835 1841
Size: 237 351 297 279 507 13 44 6
Cuts at: 1841 2155 2509 107
Size: 314 354 2619
Fragments arranged by size:
2619 507 354 351 314 297 279 237
44 13 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 667 1258 1742 2604 2737 3196 3405 667
Size: 591 484 862 133 459 209 2283
Fragments arranged by size:
2283 862 591 484 459 209 133
TatI w'GTAC_w
Cuts at: 4536 4536
Size: 5021
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 387 419 1376 1408 4538 4570 387
Size: 32 957 32 3130 32 838
Fragments arranged by size:
3130 957 838 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2818 2818
Size: 5021
XbaI T'CTAG_A
Cuts at: 2052 2052
Size: 5021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1230 1776 2516 1230
Size: 546 740 3735
Fragments arranged by size:
3735 740 546
XmaI C'CCGG_G
Cuts at: 2041 2041
Size: 5021
XmnI GAAnn'nnTTC
Cuts at: 1510 1510
Size: 5021
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI