Restriction map for
pF1KA0110
(Circular) (Six-base) MAPSORT of: pF1KA0110.seq Check: 8027 from: 1 to: 3935
pF1KA0110 3935 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 973 973
Size: 3935
Acc65I G'GTAC_C
Cuts at: 273 951 273
Size: 678 3257
AclI AA'CG_TT
Cuts at: 3775 3775
Size: 3935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 700 1760 2192 2545 3207 454
Size: 246 1060 432 353 662 1182
Fragments arranged by size:
1182 1060 662 432 353 246
AflII C'TTAA_G
Cuts at: 388 388
Size: 3935
AflIII A'CryG_T
Cuts at: 1287 3087 3368 1287
Size: 1800 281 1854
Fragments arranged by size:
1854 1800 281
AleI CACnn'nnGTG
Cuts at: 460 460
Size: 3935
AlwNI CAG_nnn'CTG
Cuts at: 151 648 1240 2678 151
Size: 497 592 1438 1408
Fragments arranged by size:
1438 1408 592 497
ApaLI G'TGCA_C
Cuts at: 2773 2773
Size: 3935
ApoI r'AATT_y
Cuts at: 939 3288 3544 939
Size: 2349 256 1330
Fragments arranged by size:
2349 1330 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3935
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
AvaI C'yCGr_G
Cuts at: 446 955 446
Size: 509 3426
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 530 563 530
Size: 33 3902
BamHI G'GATC_C
Cuts at: 960 960
Size: 3935
BanI G'GyrC_C
Cuts at: 114 273 736 900 951 1365 1613 1648 114
Size: 159 463 164 51 414 248 35 2401
Fragments arranged by size:
2401 463 414 248 164 159 51 35
BanII G_rGCy'C
Cuts at: 949 1979 949
Size: 1030 2905
BbeI G_GCGC'C
Cuts at: 118 1369 1617 118
Size: 1251 248 2436
Fragments arranged by size:
2436 1251 248
BbsI GAAGACnn'nnnn_
Cuts at: 887 887
Size: 3935
BbvCI CC'TCA_GC
Cuts at: 138 511 138
Size: 373 3562
BciVI GTATCCnnnnn_n'
Cuts at: 1833 2889 3935 1833
Size: 1056 1046 1833
Fragments arranged by size:
1833 1056 1046
BclI T'GATC_A
Cuts at: 1455 1455
Size: 3935
BglI GCCn_nnn'nGGC
Cuts at: 112 308 112
Size: 196 3739
BglII A'GATC_T
Cuts at: 1450 3487 1450
Size: 2037 1898
BlpI GC'TnA_GC
Cuts at: 1047 1047
Size: 3935
Bme1580I G_kGCm'C
Cuts at: 739 812 1560 1653 2777 739
Size: 73 748 93 1124 1897
Fragments arranged by size:
1897 1124 748 93 73
BmrI ACTGGGnnnn_n'
Cuts at: 1147 1228 1563 3707 1147
Size: 81 335 2144 1375
Fragments arranged by size:
2144 1375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 234 2302 234
Size: 2068 1867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 541 520
Size: 21 3914
Bpu10I CC'TnA_GC
Cuts at: 138 511 583 649 138
Size: 373 72 66 3424
Fragments arranged by size:
3424 373 72 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1111 1990 2704 3002 229
Size: 882 879 714 298 1162
Fragments arranged by size:
1162 882 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3639 3639
Size: 3935
BsaAI yAC'GTr
Cuts at: 1288 1288
Size: 3935
BsaBI GATnn'nnATC
Cuts at: 6 1449 3486 6
Size: 1443 2037 455
Fragments arranged by size:
2037 1443 455
BsaHI Gr'CG_yC
Cuts at: 115 1366 1614 2316 3812 115
Size: 1251 248 702 1496 238
Fragments arranged by size:
1496 1251 702 248 238
BsaWI w'CCGG_w
Cuts at: 1124 1645 2323 2734 2881 3155 1124
Size: 521 678 411 147 274 1904
Fragments arranged by size:
1904 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 118 148 118
Size: 30 3905
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 713 439
Size: 274 3661
BseYI C'CCAG_C
Cuts at: 811 1360 2118 2783 3191 811
Size: 549 758 665 408 1555
Fragments arranged by size:
1555 758 665 549 408
BsiEI CG_ry'CG
Cuts at: 104 1477 1523 2753 104
Size: 1373 46 1230 1286
Fragments arranged by size:
1373 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 655 949 1727 1917 2777 655
Size: 294 778 190 860 1813
Fragments arranged by size:
1813 860 778 294 190
BsmBI CGTCTCn'nnnn_
Cuts at: 679 679
Size: 3935
Bsp1286I G_dGCh'C
Cuts at: 655 739 812 949 1560 1653 1727 1917 1979
Size: 84 73 137 611 93 74 190 62
Cuts at: 1979 2777 655
Size: 798 1813
Fragments arranged by size:
1813 798 611 190 137 93 84 74
73 62
BspHI T'CATG_A
Cuts at: 2367 2367
Size: 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 985 1199 1501 1882 985
Size: 214 302 381 3038
Fragments arranged by size:
3038 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2227 2281 3765 3930 50
Size: 2177 54 1484 165 55
Fragments arranged by size:
2177 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 582 1847 582
Size: 1265 2670
BsrFI r'CCGG_y
Cuts at: 1933 2114 1933
Size: 181 3754
BssSI C'ACGA_G
Cuts at: 2206 2914 2206
Size: 708 3227
BstAPI GCAn_nnn'nTGC
Cuts at: 476 1312 476
Size: 836 3099
BstBI TT'CG_AA
Cuts at: 2296 2296
Size: 3935
BstXI CCAn_nnnn'nTGG
Cuts at: 693 693
Size: 3935
BstYI r'GATC_y
Cuts at: 1 960 1427 1450 1785 2031 2337 2349 2435
Size: 959 467 23 335 246 306 12 86
Cuts at: 2435 3487 1
Size: 1052 449
Fragments arranged by size:
1052 959 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 869 107
Size: 762 3173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1885 2066 2229 1885
Size: 181 163 3591
Fragments arranged by size:
3591 181 163
BtsI GCAGTG_nn'
Cuts at: 1178 1178
Size: 3935
DraI TTT'AAA
Cuts at: 915 934 915
Size: 19 3916
DrdI GACnn_nn'nnGTC
Cuts at: 1641 2985 1641
Size: 1344 2591
EaeI y'GGCC_r
Cuts at: 1520 1694 2085 2112 3457 1520
Size: 174 391 27 1345 1998
Fragments arranged by size:
1998 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1520 1520
Size: 3935
EarI CTCTTCn'nnn_
Cuts at: 682 1958 2168 682
Size: 1276 210 2449
Fragments arranged by size:
2449 1276 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 2143 2885 3031 3745 139
Size: 2004 742 146 714 329
Fragments arranged by size:
2004 742 714 329 146
EcoICRI GAG'CTC
Cuts at: 947 947
Size: 3935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 520 541 700 1760 2192 2545 3207 454
Size: 66 21 159 1060 432 353 662 1182
Fragments arranged by size:
1182 1060 662 432 353 159 66 21
EcoO109I rG'GnC_Cy
Cuts at: 1074 1074
Size: 3935
EcoRI G'AATT_C
Cuts at: 939 939
Size: 3935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1954 1954
Size: 3935
FspI TGC'GCA
Cuts at: 1716 1716
Size: 3935
HaeII r_GCGC'y
Cuts at: 118 1369 1617 2847 3618 118
Size: 1251 248 1230 771 435
Fragments arranged by size:
1251 1230 771 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 779 811 845 877 1777 1807 1809
Size: 32 629 32 34 32 900 30 2
Cuts at: 1809 1839 118
Size: 30 2214
Fragments arranged by size:
2214 900 629 34 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 974 974
Size: 3935
KasI G'GCGC_C
Cuts at: 114 1365 1613 114
Size: 1251 248 2436
Fragments arranged by size:
2436 1251 248
KpnI G_GTAC'C
Cuts at: 277 955 277
Size: 678 3257
MluI A'CGCG_T
Cuts at: 3368 3368
Size: 3935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2694 2878 2694
Size: 184 3751
MscI TGG'CCA
Cuts at: 1696 1696
Size: 3935
MslI CAynn'nnrTG
Cuts at: 460 691 1924 2051 460
Size: 231 1233 127 2344
Fragments arranged by size:
2344 1233 231 127
MspA1I CmG'CkG
Cuts at: 1046 1360 1720 2504 2749 1046
Size: 314 360 784 245 2232
Fragments arranged by size:
2232 784 360 314 245
NaeI GCC'GGC
Cuts at: 2116 2116
Size: 3935
NarI GG'CG_CC
Cuts at: 115 1366 1614 115
Size: 1251 248 2436
Fragments arranged by size:
2436 1251 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3935
NgoMIV G'CCGG_C
Cuts at: 2114 2114
Size: 3935
NspI r_CATG'y
Cuts at: 988 1488 3091 988
Size: 500 1603 1832
Fragments arranged by size:
1832 1603 500
PciI A'CATG_T
Cuts at: 3087 3087
Size: 3935
PflMI CCAn_nnn'nTGG
Cuts at: 875 875
Size: 3935
PmeI GTTT'AAAC
Cuts at: 934 934
Size: 3935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2381 2413 2381
Size: 32 3903
PstI C_TGCA'G
Cuts at: 982 1667 982
Size: 685 3250
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3935
PvuII CAG'CTG
Cuts at: 1360 1720 1360
Size: 360 3575
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 3935
SacI G_AGCT'C
Cuts at: 949 949
Size: 3935
SalI G'TCGA_C
Cuts at: 972 972
Size: 3935
SapI GCTCTTCn'nnn_
Cuts at: 1958 2168 1958
Size: 210 3725
SbfI CC_TGCA'GG
Cuts at: 982 982
Size: 3935
ScaI AGT'ACT
Cuts at: 3452 3452
Size: 3935
SfcI C'TryA_G
Cuts at: 33 630 978 1663 2631 2822 33
Size: 597 348 685 968 191 1146
Fragments arranged by size:
1146 968 685 597 348 191
SfoI GGC'GCC
Cuts at: 116 1367 1615 116
Size: 1251 248 2436
Fragments arranged by size:
2436 1251 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
SmaI CCC'GGG
Cuts at: 957 957
Size: 3935
SmlI C'TyrA_G
Cuts at: 208 388 1090 2005 2719 2981 208
Size: 180 702 915 714 262 1162
Fragments arranged by size:
1162 915 714 702 262 180
SpeI A'CTAG_T
Cuts at: 67 543 67
Size: 476 3459
SphI G_CATG'C
Cuts at: 988 1488 988
Size: 500 3435
StuI AGG'CCT
Cuts at: 250 250
Size: 3935
StyI C'CwwG_G
Cuts at: 107 567 590 1069 1423 107
Size: 460 23 479 354 2619
Fragments arranged by size:
2619 479 460 354 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1518 1651 2110 2319 1518
Size: 133 459 209 3134
Fragments arranged by size:
3134 459 209 133
TatI w'GTAC_w
Cuts at: 3450 3450
Size: 3935
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 3903
Tth111I GACn'n_nGTC
Cuts at: 840 1732 840
Size: 892 3043
XbaI T'CTAG_A
Cuts at: 966 966
Size: 3935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1430 1430
Size: 3935
XmaI C'CCGG_G
Cuts at: 955 955
Size: 3935
XmnI GAAnn'nnTTC
Cuts at: 408 408
Size: 3935
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI ApaI
AscI AvrII BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI