Restriction map for
pF1KA0103
(Circular) (Six-base) MAPSORT of: pF1KA0103.seq Check: 236 from: 1 to: 4004
pF1KA0103 4004 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 706 1042 706
Size: 336 3668
Acc65I G'GTAC_C
Cuts at: 1020 1020
Size: 4004
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 716 1829 2261 2614 3276 716
Size: 1113 432 353 662 1444
Fragments arranged by size:
1444 1113 662 432 353
AflII C'TTAA_G
Cuts at: 952 952
Size: 4004
AflIII A'CryG_T
Cuts at: 968 1356 3156 3437 968
Size: 388 1800 281 1535
Fragments arranged by size:
1800 1535 388 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 605 605
Size: 4004
AlwNI CAG_nnn'CTG
Cuts at: 1309 2747 1309
Size: 1438 2566
ApaLI G'TGCA_C
Cuts at: 2842 2842
Size: 4004
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 226 230 20
Size: 206 4 3794
Fragments arranged by size:
3794 206 4
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 1024 1024
Size: 4004
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 1020 1434 1682 1717 1020
Size: 414 248 35 3307
Fragments arranged by size:
3307 414 248 35
BanII G_rGCy'C
Cuts at: 1018 2048 1018
Size: 1030 2974
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BbsI GAAGACnn'nnnn_
Cuts at: 325 354 971 325
Size: 29 617 3358
Fragments arranged by size:
3358 617 29
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2958 4004 1902
Size: 1056 1046 1902
Fragments arranged by size:
1902 1056 1046
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 1629 1722 2846 1629
Size: 93 1124 2787
Fragments arranged by size:
2787 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 987 1216 1297 1632 3776 987
Size: 229 81 335 2144 1215
Fragments arranged by size:
2144 1215 335 229 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 1180 2059 2773 3071 171
Size: 1009 879 714 298 1104
Fragments arranged by size:
1104 1009 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 123 560 3708 123
Size: 437 3148 419
Fragments arranged by size:
3148 437 419
BsaAI yAC'GTr
Cuts at: 1357 1357
Size: 4004
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2803 2950 3224 1193
Size: 521 678 411 147 274 1973
Fragments arranged by size:
1973 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 249 1429 2187 2852 3260 249
Size: 1180 758 665 408 993
Fragments arranged by size:
1180 993 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2822 104
Size: 1442 46 1230 1286
Fragments arranged by size:
1442 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1018 1796 1986 2846 1018
Size: 778 190 860 2176
Fragments arranged by size:
2176 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1018 1629 1722 1796 1986 2048 2846 1018
Size: 611 93 74 190 62 798 2176
Fragments arranged by size:
2176 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2436 2436
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 910 1054 1268 1570 1951 910
Size: 144 214 302 381 2963
Fragments arranged by size:
2963 381 302 214 144
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 979 1916 979
Size: 937 3067
BsrFI r'CCGG_y
Cuts at: 2002 2183 2002
Size: 181 3823
BssSI C'ACGA_G
Cuts at: 2275 2983 2275
Size: 708 3296
BstAPI GCAn_nnn'nTGC
Cuts at: 1381 1381
Size: 4004
BstBI TT'CG_AA
Cuts at: 498 2365 498
Size: 1867 2137
BstYI r'GATC_y
Cuts at: 1 456 1029 1496 1519 1854 2100 2406 2418
Size: 455 573 467 23 335 246 306 12
Cuts at: 2418 2504 3556 1
Size: 86 1052 449
Fragments arranged by size:
1052 573 467 455 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 707 707
Size: 4004
BtgI C'CryG_G
Cuts at: 107 404 107
Size: 297 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 467 1247 467
Size: 780 3224
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 3054 1710
Size: 1344 2660
EaeI y'GGCC_r
Cuts at: 1589 1763 2154 2181 3526 1589
Size: 174 391 27 1345 2067
Fragments arranged by size:
2067 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2212 2954 3100 3814 2212
Size: 742 146 714 2402
Fragments arranged by size:
2402 742 714 146
EcoICRI GAG'CTC
Cuts at: 1016 1016
Size: 4004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 716 1829 2261 2614 3276 716
Size: 1113 432 353 662 1444
Fragments arranged by size:
1444 1113 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1143 1143
Size: 4004
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 736 2023 736
Size: 1287 2717
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2916 3687 1438
Size: 248 1230 771 1755
Fragments arranged by size:
1755 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 1846 1876 1878 1908 645
Size: 32 1169 30 2 30 2741
Fragments arranged by size:
2741 1169 32 30 30 2
HincII GTy'rAC
Cuts at: 1043 1043
Size: 4004
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 1024 1024
Size: 4004
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 195 484 543 953 2763 2947 195
Size: 289 59 410 1810 184 1252
Fragments arranged by size:
1810 1252 410 289 184 59
MscI TGG'CCA
Cuts at: 1765 1765
Size: 4004
MslI CAynn'nnrTG
Cuts at: 617 1993 2120 617
Size: 1376 127 2501
Fragments arranged by size:
2501 1376 127
MspA1I CmG'CkG
Cuts at: 1115 1429 1789 2573 2818 1115
Size: 314 360 784 245 2301
Fragments arranged by size:
2301 784 360 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NcoI C'CATG_G
Cuts at: 107 404 107
Size: 297 3707
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 972 1057 1557 3160 972
Size: 85 500 1603 1816
Fragments arranged by size:
1816 1603 500 85
PacI TTA_AT'TAA
Cuts at: 230 230
Size: 4004
PasI CC'CwG_GG
Cuts at: 507 507
Size: 4004
PciI A'CATG_T
Cuts at: 968 3156 968
Size: 2188 1816
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2450 2482 2450
Size: 32 3972
PshAI GACnn'nnGTC
Cuts at: 305 305
Size: 4004
PstI C_TGCA'G
Cuts at: 1051 1736 1051
Size: 685 3319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 1429 1789 1429
Size: 360 3644
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 1018 1018
Size: 4004
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 1047 1732 2700 2891 33
Size: 1014 685 968 191 1146
Fragments arranged by size:
1146 1014 968 685 191
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 1026 1026
Size: 4004
SmlI C'TyrA_G
Cuts at: 186 952 1159 2074 2788 3050 186
Size: 766 207 915 714 262 1140
Fragments arranged by size:
1140 915 766 714 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
SspI AAT'ATT
Cuts at: 946 946
Size: 4004
StyI C'CwwG_G
Cuts at: 107 404 711 1138 1492 107
Size: 297 307 427 354 2619
Fragments arranged by size:
2619 427 354 307 297
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1006 1587 1720 2179 2388 1006
Size: 581 133 459 209 2622
Fragments arranged by size:
2622 581 459 209 133
TatI w'GTAC_w
Cuts at: 3519 3519
Size: 4004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 3972
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1499 1499
Size: 4004
XmaI C'CCGG_G
Cuts at: 1024 1024
Size: 4004
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PacI PasI PciI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PflMI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI