Restriction map for
pF1KA0101
(Circular) (Six-base) MAPSORT of: pF1KA0101.seq Check: 8737 from: 1 to: 3446
pF1KA0101 3446 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 484 484
Size: 3446
Acc65I G'GTAC_C
Cuts at: 462 462
Size: 3446
AclI AA'CG_TT
Cuts at: 3286 3286
Size: 3446
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 1271 1703 2056 2718 377
Size: 894 432 353 662 1105
Fragments arranged by size:
1105 894 662 432 353
AflIII A'CryG_T
Cuts at: 798 2598 2879 798
Size: 1800 281 1365
Fragments arranged by size:
1800 1365 281
AlwNI CAG_nnn'CTG
Cuts at: 751 2189 751
Size: 1438 2008
ApaLI G'TGCA_C
Cuts at: 2284 2284
Size: 3446
ApoI r'AATT_y
Cuts at: 307 450 2799 3055 307
Size: 143 2349 256 698
Fragments arranged by size:
2349 698 256 143
AseI AT'TA_AT
Cuts at: 20 20
Size: 3446
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3446
AvaI C'yCGr_G
Cuts at: 164 466 164
Size: 302 3144
BamHI G'GATC_C
Cuts at: 471 471
Size: 3446
BanI G'GyrC_C
Cuts at: 462 876 1124 1159 462
Size: 414 248 35 2749
Fragments arranged by size:
2749 414 248 35
BanII G_rGCy'C
Cuts at: 171 460 1490 171
Size: 289 1030 2127
Fragments arranged by size:
2127 1030 289
BbeI G_GCGC'C
Cuts at: 880 1128 880
Size: 248 3198
BciVI GTATCCnnnnn_n'
Cuts at: 1344 2400 3446 1344
Size: 1056 1046 1344
Fragments arranged by size:
1344 1056 1046
BclI T'GATC_A
Cuts at: 417 966 417
Size: 549 2897
BglII A'GATC_T
Cuts at: 961 2998 961
Size: 2037 1409
BlpI GC'TnA_GC
Cuts at: 558 558
Size: 3446
Bme1580I G_kGCm'C
Cuts at: 1071 1164 2288 1071
Size: 93 1124 2229
Fragments arranged by size:
2229 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 658 739 1074 3218 658
Size: 81 335 2144 886
Fragments arranged by size:
2144 886 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1813 1813
Size: 3446
Bpu10I CC'TnA_GC
Cuts at: 378 378
Size: 3446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1501 2215 2513 622
Size: 879 714 298 1555
Fragments arranged by size:
1555 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3150 3150
Size: 3446
BsaAI yAC'GTr
Cuts at: 799 799
Size: 3446
BsaBI GATnn'nnATC
Cuts at: 6 960 2997 6
Size: 954 2037 455
Fragments arranged by size:
2037 954 455
BsaHI Gr'CG_yC
Cuts at: 877 1125 1827 3323 877
Size: 248 702 1496 1000
Fragments arranged by size:
1496 1000 702 248
BsaWI w'CCGG_w
Cuts at: 635 1156 1834 2245 2392 2666 635
Size: 521 678 411 147 274 1415
Fragments arranged by size:
1415 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 871 1629 2294 2702 871
Size: 758 665 408 1615
Fragments arranged by size:
1615 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 988 1034 2264 104
Size: 884 46 1230 1286
Fragments arranged by size:
1286 1230 884 46
BsiHKAI G_wGCw'C
Cuts at: 460 1238 1428 2288 460
Size: 778 190 860 1618
Fragments arranged by size:
1618 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 171 460 1071 1164 1238 1428 1490 2288 171
Size: 289 611 93 74 190 62 798 1329
Fragments arranged by size:
1329 798 611 289 190 93 74 62
BspHI T'CATG_A
Cuts at: 1878 1878
Size: 3446
BspMI ACCTGCnnnn'nnnn_
Cuts at: 496 710 1012 1393 496
Size: 214 302 381 2549
Fragments arranged by size:
2549 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1738 1792 3276 3441 50
Size: 1688 54 1484 165 55
Fragments arranged by size:
1688 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1358 1358
Size: 3446
BsrFI r'CCGG_y
Cuts at: 1444 1625 1444
Size: 181 3265
BssSI C'ACGA_G
Cuts at: 1717 2425 1717
Size: 708 2738
BstAPI GCAn_nnn'nTGC
Cuts at: 823 823
Size: 3446
BstBI TT'CG_AA
Cuts at: 1807 1807
Size: 3446
BstYI r'GATC_y
Cuts at: 1 471 938 961 1296 1542 1848 1860 1946
Size: 470 467 23 335 246 306 12 86
Cuts at: 1946 2998 1
Size: 1052 449
Fragments arranged by size:
1052 470 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1396 1577 1740 1396
Size: 181 163 3102
Fragments arranged by size:
3102 181 163
BtsI GCAGTG_nn'
Cuts at: 378 689 378
Size: 311 3135
DraI TTT'AAA
Cuts at: 445 445
Size: 3446
DrdI GACnn_nn'nnGTC
Cuts at: 1152 2496 1152
Size: 1344 2102
EaeI y'GGCC_r
Cuts at: 1031 1205 1596 1623 2968 1031
Size: 174 391 27 1345 1509
Fragments arranged by size:
1509 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1031 1031
Size: 3446
EarI CTCTTCn'nnn_
Cuts at: 353 1469 1679 353
Size: 1116 210 2120
Fragments arranged by size:
2120 1116 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1654 2396 2542 3256 1654
Size: 742 146 714 1844
Fragments arranged by size:
1844 742 714 146
EcoICRI GAG'CTC
Cuts at: 458 458
Size: 3446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 1271 1703 2056 2718 377
Size: 894 432 353 662 1105
Fragments arranged by size:
1105 894 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 585 585
Size: 3446
EcoRI G'AATT_C
Cuts at: 307 450 307
Size: 143 3303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1465 1465
Size: 3446
FspI TGC'GCA
Cuts at: 1227 1227
Size: 3446
HaeII r_GCGC'y
Cuts at: 880 1128 2358 3129 880
Size: 248 1230 771 1197
Fragments arranged by size:
1230 1197 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1288 1318 1320 1350 1288
Size: 30 2 30 3384
Fragments arranged by size:
3384 30 30 2
HincII GTy'rAC
Cuts at: 485 485
Size: 3446
KasI G'GCGC_C
Cuts at: 876 1124 876
Size: 248 3198
KpnI G_GTAC'C
Cuts at: 466 466
Size: 3446
MluI A'CGCG_T
Cuts at: 2879 2879
Size: 3446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2205 2389 2205
Size: 184 3262
MscI TGG'CCA
Cuts at: 1207 1207
Size: 3446
MslI CAynn'nnrTG
Cuts at: 425 1435 1562 425
Size: 1010 127 2309
Fragments arranged by size:
2309 1010 127
MspA1I CmG'CkG
Cuts at: 557 871 1231 2015 2260 557
Size: 314 360 784 245 1743
Fragments arranged by size:
1743 784 360 314 245
NaeI GCC'GGC
Cuts at: 1627 1627
Size: 3446
NarI GG'CG_CC
Cuts at: 877 1125 877
Size: 248 3198
NcoI C'CATG_G
Cuts at: 107 107
Size: 3446
NgoMIV G'CCGG_C
Cuts at: 1625 1625
Size: 3446
NspI r_CATG'y
Cuts at: 404 499 999 2602 404
Size: 95 500 1603 1248
Fragments arranged by size:
1603 1248 500 95
PciI A'CATG_T
Cuts at: 2598 2598
Size: 3446
PmeI GTTT'AAAC
Cuts at: 445 445
Size: 3446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1892 1924 1892
Size: 32 3414
PspXI vC'TCGA_Gb
Cuts at: 164 164
Size: 3446
PstI C_TGCA'G
Cuts at: 493 1178 493
Size: 685 2761
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3446
PvuII CAG'CTG
Cuts at: 871 1231 871
Size: 360 3086
RsrII CG'GwC_CG
Cuts at: 1641 1641
Size: 3446
SacI G_AGCT'C
Cuts at: 460 460
Size: 3446
SalI G'TCGA_C
Cuts at: 483 483
Size: 3446
SapI GCTCTTCn'nnn_
Cuts at: 1469 1679 1469
Size: 210 3236
SbfI CC_TGCA'GG
Cuts at: 493 493
Size: 3446
ScaI AGT'ACT
Cuts at: 2963 2963
Size: 3446
SfcI C'TryA_G
Cuts at: 33 489 1174 2142 2333 33
Size: 456 685 968 191 1146
Fragments arranged by size:
1146 968 685 456 191
SfoI GGC'GCC
Cuts at: 878 1126 878
Size: 248 3198
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3446
SmaI CCC'GGG
Cuts at: 468 468
Size: 3446
SmlI C'TyrA_G
Cuts at: 164 601 1516 2230 2492 164
Size: 437 915 714 262 1118
Fragments arranged by size:
1118 915 714 437 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3446
SphI G_CATG'C
Cuts at: 499 999 499
Size: 500 2946
StyI C'CwwG_G
Cuts at: 107 580 934 107
Size: 473 354 2619
Fragments arranged by size:
2619 473 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1029 1162 1621 1830 1029
Size: 133 459 209 2645
Fragments arranged by size:
2645 459 209 133
TatI w'GTAC_w
Cuts at: 2961 2961
Size: 3446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3414
Tth111I GACn'n_nGTC
Cuts at: 1243 1243
Size: 3446
XbaI T'CTAG_A
Cuts at: 477 477
Size: 3446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 284 941 284
Size: 657 2789
XhoI C'TCGA_G
Cuts at: 164 164
Size: 3446
XmaI C'CCGG_G
Cuts at: 466 466
Size: 3446
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmgBI BmtI BpmI BsaXI BseRI BsgI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI