Restriction map for
pF1KA0100
(Circular) (Six-base) MAPSORT of: pF1KA0100.seq Check: 2347 from: 1 to: 9818
pF1KA0100 9818 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 700 1179 1286 3725 4358 700
Size: 479 107 2439 633 6160
Fragments arranged by size:
6160 2439 633 479 107
AatII G_ACGT'C
Cuts at: 1933 1933
Size: 9818
AccI GT'mk_AC
Cuts at: 3237 6856 3237
Size: 3619 6199
Acc65I G'GTAC_C
Cuts at: 3200 6834 3200
Size: 3634 6184
AclI AA'CG_TT
Cuts at: 9658 9658
Size: 9818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 1036 1681 1868 2216 2671 4322 4591 4653
Size: 580 645 187 348 455 1651 269 62
Cuts at: 4653 5321 5665 6060 6676 7643 8075 8822 9090
Size: 668 344 395 616 967 432 747 268
Cuts at: 9090 456
Size: 1184
Fragments arranged by size:
1651 1184 967 747 668 645 616 580
455 432 395 348 344 269 268 187
62
AfeI AGC'GCT
Cuts at: 444 1964 444
Size: 1520 8298
AflII C'TTAA_G
Cuts at: 277 277
Size: 9818
AflIII A'CryG_T
Cuts at: 2132 4709 7170 8274 9251 2132
Size: 2577 2461 1104 977 2699
Fragments arranged by size:
2699 2577 2461 1104 977
AgeI A'CCGG_T
Cuts at: 8206 8206
Size: 9818
AleI CACnn'nnGTG
Cuts at: 967 3391 3782 967
Size: 2424 391 7003
Fragments arranged by size:
7003 2424 391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 776 1126 4231 5027 5566 6311 776
Size: 350 3105 796 539 745 4283
Fragments arranged by size:
4283 3105 796 745 539 350
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5723 5755 5723
Size: 32 9786
AlwNI CAG_nnn'CTG
Cuts at: 930 1032 1638 2838 3753 5118 6673 7123 8690
Size: 102 606 1200 915 1365 1555 450 1567
Cuts at: 8690 930
Size: 2058
Fragments arranged by size:
2058 1567 1555 1365 1200 915 606 450
102
ApaI G_GGCC'C
Cuts at: 841 3403 3801 6325 841
Size: 2562 398 2524 4334
Fragments arranged by size:
4334 2562 2524 398
ApaLI G'TGCA_C
Cuts at: 8588 8588
Size: 9818
ApoI r'AATT_y
Cuts at: 1258 3349 3929 5390 6822 9171 9427 1258
Size: 2091 580 1461 1432 2349 256 1649
Fragments arranged by size:
2349 2091 1649 1461 1432 580 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9818
AvaI C'yCGr_G
Cuts at: 4175 6838 4175
Size: 2663 7155
BamHI G'GATC_C
Cuts at: 260 3411 3573 4428 6843 260
Size: 3151 162 855 2415 3235
Fragments arranged by size:
3235 3151 2415 855 162
BanI G'GyrC_C
Cuts at: 998 1250 1554 3200 3247 3819 4220 5861 6138
Size: 252 304 1646 47 572 401 1641 277
Cuts at: 6138 6834 7248 7496 7531 998
Size: 696 414 248 35 3285
Fragments arranged by size:
3285 1646 1641 696 572 414 401 304
277 252 248 47 35
BanII G_rGCy'C
Cuts at: 841 2122 2909 3403 3801 4412 6040 6325 6832
Size: 1281 787 494 398 611 1628 285 507
Cuts at: 6832 7862 841
Size: 1030 2797
Fragments arranged by size:
2797 1628 1281 1030 787 611 507 494
398 285
BbeI G_GCGC'C
Cuts at: 1558 4224 5865 7252 7500 1558
Size: 2666 1641 1387 248 3876
Fragments arranged by size:
3876 2666 1641 1387 248
BbsI GAAGACnn'nnnn_
Cuts at: 1850 3499 4161 1850
Size: 1649 662 7507
Fragments arranged by size:
7507 1649 662
BbvCI CC'TCA_GC
Cuts at: 2342 3462 4400 4638 5183 2342
Size: 1120 938 238 545 6977
Fragments arranged by size:
6977 1120 938 545 238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 4372 4406 90
Size: 34 4248 34 5502
Fragments arranged by size:
5502 4248 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 546 5352 7716 8477 9818 546
Size: 4806 2364 761 1341 546
Fragments arranged by size:
4806 2364 1341 761 546
BclI T'GATC_A
Cuts at: 3774 4314 7338 3774
Size: 540 3024 6254
Fragments arranged by size:
6254 3024 540
BfrBI ATG'CAT
Cuts at: 1575 1575
Size: 9818
BglII A'GATC_T
Cuts at: 491 3123 3287 5328 5513 7333 9370 491
Size: 2632 164 2041 185 1820 2037 939
Fragments arranged by size:
2632 2041 2037 1820 939 185 164
BlpI GC'TnA_GC
Cuts at: 154 2032 3009 4354 6930 154
Size: 1878 977 1345 2576 3042
Fragments arranged by size:
3042 2576 1878 1345 977
Bme1580I G_kGCm'C
Cuts at: 841 1725 2265 2994 3250 3403 3801 3824 6325
Size: 884 540 729 256 153 398 23 2501
Cuts at: 6325 7443 7536 8592 841
Size: 1118 93 1056 2067
Fragments arranged by size:
2501 2067 1118 1056 884 729 540 398
256 153 93 23
BmrI ACTGGGnnnn_n'
Cuts at: 1650 2020 3612 6328 6528 7030 7111 7446 9590
Size: 370 1592 2716 200 502 81 335 2144
Cuts at: 9590 1650
Size: 1878
Fragments arranged by size:
2716 2144 1878 1592 502 370 335 200
81
BmtI G_CTAG'C
Cuts at: 1285 6064 1285
Size: 4779 5039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2754 4428 8185 2754
Size: 1674 3757 4387
Fragments arranged by size:
4387 3757 1674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 907 2101 2326 5620 5986 6571 703
Size: 204 1194 225 3294 366 585 3950
Fragments arranged by size:
3950 3294 1194 585 366 225 204
Bpu10I CC'TnA_GC
Cuts at: 340 2267 2342 2489 3059 3462 4400 4638 5183
Size: 1927 75 147 570 403 938 238 545
Cuts at: 5183 5462 5849 6649 340
Size: 279 387 800 3509
Fragments arranged by size:
3509 1927 938 800 570 545 403 387
279 238 147 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1159 3136 4184 5329 5530 6994 7873 8365
Size: 630 1977 1048 1145 201 1464 879 492
Cuts at: 8365 8663 529
Size: 298 1684
Fragments arranged by size:
1977 1684 1464 1145 1048 879 630 492
298 201
BsaI GGTCTCn'nnnn_
Cuts at: 2168 9522 2168
Size: 7354 2464
BsaAI yAC'GTr
Cuts at: 7171 7171
Size: 9818
BsaBI GATnn'nnATC
Cuts at: 6 1049 4427 7332 9369 6
Size: 1043 3378 2905 2037 455
Fragments arranged by size:
3378 2905 2037 1043 455
BsaHI Gr'CG_yC
Cuts at: 1496 1555 1930 3282 4221 5862 7249 7497 8199
Size: 59 375 1352 939 1641 1387 248 702
Cuts at: 8199 9695 1496
Size: 1496 1619
Fragments arranged by size:
1641 1619 1496 1387 1352 939 702 375
248 59
BsaWI w'CCGG_w
Cuts at: 188 2513 2543 7007 7528 8206 8480 8627 9038
Size: 2325 30 4464 521 678 274 147 411
Cuts at: 9038 188
Size: 968
Fragments arranged by size:
4464 2325 968 678 521 411 274 147
30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2074 2104 2074
Size: 30 9788
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2551 2917 4417 4420 5095 5545 5986 6739 6745
Size: 366 1500 3 675 450 441 753 6
Cuts at: 6745 6748 2551
Size: 3 5621
Fragments arranged by size:
5621 1500 753 675 450 441 366 6
3 3
BseYI C'CCAG_C
Cuts at: 445 3407 3472 3920 4036 4094 4127 4663 5198
Size: 2962 65 448 116 58 33 536 535
Cuts at: 5198 5403 5886 7243 8001 8578 9074 445
Size: 205 483 1357 758 577 496 1189
Fragments arranged by size:
2962 1357 1189 758 577 536 535 496
483 448 205 116 65 58 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 1258 1943 2117 2497 3538 4173 4438 5131
Size: 51 685 174 380 1041 635 265 693
Cuts at: 5131 1207
Size: 5894
Fragments arranged by size:
5894 1041 693 685 635 380 265 174
51
BsiEI CG_ry'CG
Cuts at: 104 7360 7406 8614 104
Size: 7256 46 1208 1308
Fragments arranged by size:
7256 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1105 2033 2317 2735 2909 4412 5087 6040 6398
Size: 928 284 418 174 1503 675 953 358
Cuts at: 6398 6832 7610 7800 8592 1105
Size: 434 778 190 792 2331
Fragments arranged by size:
2331 1503 953 928 792 778 675 434
418 358 284 190 174
BsmI GAATG_Cn'
Cuts at: 3152 4314 3152
Size: 1162 8656
BsmBI CGTCTCn'nnnn_
Cuts at: 1921 2968 1921
Size: 1047 8771
Bsp1286I G_dGCh'C
Cuts at: 841 1105 1725 2033 2122 2265 2317 2735 2909
Size: 264 620 308 89 143 52 418 174
Cuts at: 2909 2994 3250 3403 3801 3824 4412 5087 6040
Size: 85 256 153 398 23 588 675 953
Cuts at: 6040 6325 6398 6832 7443 7536 7610 7800 7862
Size: 285 73 434 611 93 74 190 62
Cuts at: 7862 8592 841
Size: 730 2067
Fragments arranged by size:
2067 953 730 675 620 611 588 434
418 398 308 285 264 256 190 174
153 143 93 89 85 74 73 62
52 23
BspEI T'CCGG_A
Cuts at: 2543 9038 2543
Size: 6495 3323
BspHI T'CATG_A
Cuts at: 2778 3837 5621 8994 2778
Size: 1059 1784 3373 3602
Fragments arranged by size:
3602 3373 1784 1059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 1179 1286 3560 3725 3752 4092 4358 5450
Size: 479 107 2274 165 27 340 266 1092
Cuts at: 5450 5720 6868 7082 7384 7765 700
Size: 270 1148 214 302 381 2753
Fragments arranged by size:
2753 2274 1148 1092 479 381 340 302
270 266 214 165 107 27
BsrBI CCG'CTC
Cuts at: 50 2075 8110 8164 9648 9813 50
Size: 2025 6035 54 1484 165 55
Fragments arranged by size:
6035 2025 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3165 4499 4793 7730 3165
Size: 1334 294 2937 5253
Fragments arranged by size:
5253 2937 1334 294
BsrFI r'CCGG_y
Cuts at: 173 1247 2387 5087 7816 7997 8206 173
Size: 1074 1140 2700 2729 181 209 1785
Fragments arranged by size:
2729 2700 1785 1140 1074 209 181
BssSI C'ACGA_G
Cuts at: 2672 4418 4649 8089 8447 2672
Size: 1746 231 3440 358 4043
Fragments arranged by size:
4043 3440 1746 358 231
BstAPI GCAn_nnn'nTGC
Cuts at: 1160 7195 1160
Size: 6035 3783
BstBI TT'CG_AA
Cuts at: 1065 8179 1065
Size: 7114 2704
BstEII G'GTnAC_C
Cuts at: 1191 4272 5151 6523 1191
Size: 3081 879 1372 4486
Fragments arranged by size:
4486 3081 1372 879
BstXI CCAn_nnnn'nTGG
Cuts at: 445 3888 4789 5424 445
Size: 3443 901 635 4839
Fragments arranged by size:
4839 3443 901 635
BstYI r'GATC_y
Cuts at: 1 260 491 3123 3212 3287 3411 3573 3891
Size: 259 231 2632 89 75 124 162 318
Cuts at: 3891 4428 5328 5513 6843 7310 7333 7668 7914
Size: 537 900 185 1330 467 23 335 246
Cuts at: 7914 8926 9012 9024 9370 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2632 1330 1012 900 537 467 449 346
335 318 259 246 231 185 162 124
89 86 75 23 12
Bsu36I CC'TnA_GG
Cuts at: 1468 3113 3815 1468
Size: 1645 702 7471
Fragments arranged by size:
7471 1645 702
BtgI C'CryG_G
Cuts at: 3051 3196 3307 3516 3759 6590 3051
Size: 145 111 209 243 2831 6279
Fragments arranged by size:
6279 2831 243 209 145 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7768 7949 8112 7768
Size: 181 163 9474
Fragments arranged by size:
9474 181 163
BtsI GCAGTG_nn'
Cuts at: 758 1539 1926 2992 3329 3754 4933 5144 7061
Size: 781 387 1066 337 425 1179 211 1917
Cuts at: 7061 758
Size: 3515
Fragments arranged by size:
3515 1917 1179 1066 781 425 387 337
211
DraI TTT'AAA
Cuts at: 6817 6817
Size: 9818
DraIII CAC_nnn'GTG
Cuts at: 203 363 4651 6402 6484 203
Size: 160 4288 1751 82 3537
Fragments arranged by size:
4288 3537 1751 160 82
DrdI GACnn_nn'nnGTC
Cuts at: 1837 7524 8382 1837
Size: 5687 858 3273
Fragments arranged by size:
5687 3273 858
EaeI y'GGCC_r
Cuts at: 194 2156 2852 3432 3563 5046 6201 7403 7577
Size: 1962 696 580 131 1483 1155 1202 174
Cuts at: 7577 7968 7995 9340 194
Size: 391 27 1345 672
Fragments arranged by size:
1962 1483 1345 1202 1155 696 672 580
391 174 131 27
EagI C'GGCC_G
Cuts at: 7403 7403
Size: 9818
EarI CTCTTCn'nnn_
Cuts at: 817 1797 2492 4024 4298 4724 4754 6079 6230
Size: 980 695 1532 274 426 30 1325 151
Cuts at: 6230 6722 7841 8051 817
Size: 492 1119 210 2584
Fragments arranged by size:
2584 1532 1325 1119 980 695 492 426
274 210 151 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 241 6058 8026 8336 8482 9628 241
Size: 5817 1968 310 146 1146 431
Fragments arranged by size:
5817 1968 1146 431 310 146
EcoICRI GAG'CTC
Cuts at: 2907 4410 6038 6830 2907
Size: 1503 1628 792 5895
Fragments arranged by size:
5895 1628 1503 792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 703 907 1036 1681 1868 2101 2216 2326
Size: 247 204 129 645 187 233 115 110
Cuts at: 2326 2671 4322 4591 4653 5321 5620 5665 5986
Size: 345 1651 269 62 668 299 45 321
Cuts at: 5986 6060 6571 6676 7643 8075 8822 9090 456
Size: 74 511 105 967 432 747 268 1184
Fragments arranged by size:
1651 1184 967 747 668 645 511 432
345 321 299 269 268 247 233 204
187 129 115 110 105 74 62 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3814 4202 4267 5869 3814
Size: 388 65 1602 7763
Fragments arranged by size:
7763 1602 388 65
EcoO109I rG'GnC_Cy
Cuts at: 807 837 2007 3399 3798 4566 6322 6957 807
Size: 30 1170 1392 399 768 1756 635 3668
Fragments arranged by size:
3668 1756 1392 1170 768 635 399 30
EcoRI G'AATT_C
Cuts at: 1258 3929 6822 1258
Size: 2671 2893 4254
Fragments arranged by size:
4254 2893 2671
EcoRV GAT'ATC
Cuts at: 3669 3669
Size: 9818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1848 3678 6813 7837 1848
Size: 1830 3135 1024 3829
Fragments arranged by size:
3829 3135 1830 1024
FspI TGC'GCA
Cuts at: 7599 7599
Size: 9818
HaeII r_GCGC'y
Cuts at: 161 446 1558 1966 4224 5865 7252 7500 8522
Size: 285 1112 408 2258 1641 1387 248 1022
Cuts at: 8522 9501 161
Size: 979 478
Fragments arranged by size:
2258 1641 1387 1112 1022 979 478 408
285 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 996 1028 2362 2394 3352 3384 4675 4707 6229
Size: 32 1334 32 958 32 1291 32 1522
Cuts at: 6229 6261 7660 7690 7692 7722 996
Size: 32 1399 30 2 30 3092
Fragments arranged by size:
3092 1522 1399 1334 1291 958 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1392 3238 4656 4851 5436 6857 1392
Size: 1846 1418 195 585 1421 4353
Fragments arranged by size:
4353 1846 1421 1418 585 195
HindIII A'AGCT_T
Cuts at: 1068 5677 1068
Size: 4609 5209
HpaI GTT'AAC
Cuts at: 4656 4656
Size: 9818
KasI G'GCGC_C
Cuts at: 1554 4220 5861 7248 7496 1554
Size: 2666 1641 1387 248 3876
Fragments arranged by size:
3876 2666 1641 1387 248
KpnI G_GTAC'C
Cuts at: 3204 6838 3204
Size: 3634 6184
MluI A'CGCG_T
Cuts at: 9251 9251
Size: 9818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5790 8489 8673 5790
Size: 2699 184 6935
Fragments arranged by size:
6935 2699 184
MscI TGG'CCA
Cuts at: 196 2158 3434 3565 7579 196
Size: 1962 1276 131 4014 2435
Fragments arranged by size:
4014 2435 1962 1276 131
MslI CAynn'nnrTG
Cuts at: 521 967 1043 1818 2980 3391 3782 3886 4213
Size: 446 76 775 1162 411 391 104 327
Cuts at: 4213 6573 7807 7934 521
Size: 2360 1234 127 2405
Fragments arranged by size:
2405 2360 1234 1162 775 446 411 391
327 127 104 76
MspA1I CmG'CkG
Cuts at: 213 681 930 1056 1491 1551 1777 2481 3466
Size: 468 249 126 435 60 226 704 985
Cuts at: 3466 3561 4098 4188 4399 4452 4537 4906 5182
Size: 95 537 90 211 53 85 369 276
Cuts at: 5182 5211 5598 5718 5775 5886 6156 6291 6929
Size: 29 387 120 57 111 270 135 638
Cuts at: 6929 7243 7603 8616 8861 213
Size: 314 360 1013 245 1170
Fragments arranged by size:
1170 1013 985 704 638 537 468 435
387 369 360 314 276 270 249 245
226 211 135 126 120 111 95 90
85 60 57 53 29
NaeI GCC'GGC
Cuts at: 2389 7999 2389
Size: 5610 4208
NarI GG'CG_CC
Cuts at: 1555 4221 5862 7249 7497 1555
Size: 2666 1641 1387 248 3876
Fragments arranged by size:
3876 2666 1641 1387 248
NcoI C'CATG_G
Cuts at: 3051 3516 3759 6590 3051
Size: 465 243 2831 6279
Fragments arranged by size:
6279 2831 465 243
NgoMIV G'CCGG_C
Cuts at: 2387 7997 2387
Size: 5610 4208
NheI G'CTAG_C
Cuts at: 1281 6060 1281
Size: 4779 5039
NsiI A_TGCA'T
Cuts at: 1577 1577
Size: 9818
NspI r_CATG'y
Cuts at: 1579 2136 2152 3067 3339 4713 5004 5581 6717
Size: 557 16 915 272 1374 291 577 1136
Cuts at: 6717 6871 7371 8278 1579
Size: 154 500 907 3119
Fragments arranged by size:
3119 1374 1136 915 907 577 557 500
291 272 154 16
PasI CC'CwG_GG
Cuts at: 6325 6325
Size: 9818
PciI A'CATG_T
Cuts at: 2132 4709 8274 2132
Size: 2577 3565 3676
Fragments arranged by size:
3676 3565 2577
PflMI CCAn_nnn'nTGG
Cuts at: 203 1353 1638 1765 3441 5168 5385 203
Size: 1150 285 127 1676 1727 217 4636
Fragments arranged by size:
4636 1727 1676 1150 285 217 127
PfoI T'CCnGG_A
Cuts at: 4477 4978 5423 4477
Size: 501 445 8872
Fragments arranged by size:
8872 501 445
PmeI GTTT'AAAC
Cuts at: 6817 6817
Size: 9818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2367 8957 8989 2335
Size: 32 6590 32 3164
Fragments arranged by size:
6590 3164 32 32
PshAI GACnn'nnGTC
Cuts at: 725 3988 4265 725
Size: 3263 277 6278
Fragments arranged by size:
6278 3263 277
PspOMI G'GGCC_C
Cuts at: 837 3399 3797 6321 837
Size: 2562 398 2524 4334
Fragments arranged by size:
4334 2562 2524 398
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 9786
PstI C_TGCA'G
Cuts at: 224 1028 2370 2408 2537 3749 4009 4103 4928
Size: 804 1342 38 129 1212 260 94 825
Cuts at: 4928 5717 5774 5912 6865 7550 224
Size: 789 57 138 953 685 2492
Fragments arranged by size:
2492 1342 1212 953 825 804 789 685
260 138 129 94 57 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9818
PvuII CAG'CTG
Cuts at: 681 930 1056 1777 2481 3466 3561 4098 4399
Size: 249 126 721 704 985 95 537 301
Cuts at: 4399 4452 4537 4906 5182 5598 5718 5886 6156
Size: 53 85 369 276 416 120 168 270
Cuts at: 6156 6291 7243 7603 681
Size: 135 952 360 2896
Fragments arranged by size:
2896 985 952 721 704 537 416 369
360 301 276 270 249 168 135 126
120 95 85 53
RsrII CG'GwC_CG
Cuts at: 8013 8013
Size: 9818
SacI G_AGCT'C
Cuts at: 2909 4412 6040 6832 2909
Size: 1503 1628 792 5895
Fragments arranged by size:
5895 1628 1503 792
SalI G'TCGA_C
Cuts at: 3236 6855 3236
Size: 3619 6199
SapI GCTCTTCn'nnn_
Cuts at: 4024 7841 8051 4024
Size: 3817 210 5791
Fragments arranged by size:
5791 3817 210
SbfI CC_TGCA'GG
Cuts at: 2408 6865 2408
Size: 4457 5361
ScaI AGT'ACT
Cuts at: 5008 6023 9335 5008
Size: 1015 3312 5491
Fragments arranged by size:
5491 3312 1015
SexAI A'CCwGG_T
Cuts at: 938 3636 6533 938
Size: 2698 2897 4223
Fragments arranged by size:
4223 2897 2698
SfcI C'TryA_G
Cuts at: 33 220 403 1024 1615 2164 2366 2404 2533
Size: 187 183 621 591 549 202 38 129
Cuts at: 2533 2830 3745 4005 4099 4924 5713 5770 5908
Size: 297 915 260 94 825 789 57 138
Cuts at: 5908 5922 6861 7546 8539 8730 33
Size: 14 939 685 993 191 1121
Fragments arranged by size:
1121 993 939 915 825 789 685 621
591 549 297 260 202 191 187 183
138 129 94 57 38 14
SfoI GGC'GCC
Cuts at: 1556 4222 5863 7250 7498 1556
Size: 2666 1641 1387 248 3876
Fragments arranged by size:
3876 2666 1641 1387 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9818
SmaI CCC'GGG
Cuts at: 6840 6840
Size: 9818
SmlI C'TyrA_G
Cuts at: 277 544 1174 3151 4199 5308 5509 6973 7888
Size: 267 630 1977 1048 1109 201 1464 915
Cuts at: 7888 8380 8642 277
Size: 492 262 1453
Fragments arranged by size:
1977 1464 1453 1109 1048 915 630 492
267 262 201
SpeI A'CTAG_T
Cuts at: 67 4630 67
Size: 4563 5255
SphI G_CATG'C
Cuts at: 5004 6871 7371 5004
Size: 1867 500 7451
Fragments arranged by size:
7451 1867 500
SspI AAT'ATT
Cuts at: 3377 3377
Size: 9818
StuI AGG'CCT
Cuts at: 3999 3999
Size: 9818
StyI C'CwwG_G
Cuts at: 1296 1430 1871 3051 3516 3759 3824 4331 6590
Size: 134 441 1180 465 243 65 507 2259
Cuts at: 6590 6952 7306 1296
Size: 362 354 3808
Fragments arranged by size:
3808 2259 1180 507 465 441 362 354
243 134 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1577 1581 1669 2994 4311 7401 7534 7993 8202
Size: 4 88 1325 1317 3090 133 459 209
Cuts at: 8202 1577
Size: 3193
Fragments arranged by size:
3193 3090 1325 1317 459 209 133 88
4
TatI w'GTAC_w
Cuts at: 3383 4539 5006 5240 6021 9333 3383
Size: 1156 467 234 781 3312 3868
Fragments arranged by size:
3868 3312 1156 781 467 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1466 1498 1685 1717 2074 2106 2181 2213 3558
Size: 32 187 32 357 32 75 32 1345
Cuts at: 3558 3590 3733 3765 6257 6289 9335 9367 1466
Size: 32 143 32 2492 32 3046 32 1917
Fragments arranged by size:
3046 2492 1917 1345 357 187 143 75
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 940 5431 7615 940
Size: 4491 2184 3143
Fragments arranged by size:
4491 3143 2184
XbaI T'CTAG_A
Cuts at: 2619 6849 2619
Size: 4230 5588
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3485 5715 7313 3485
Size: 2230 1598 5990
Fragments arranged by size:
5990 2230 1598
XmaI C'CCGG_G
Cuts at: 6838 6838
Size: 9818
XmnI GAAnn'nnTTC
Cuts at: 493 2241 2521 3077 5960 493
Size: 1748 280 556 2883 4351
Fragments arranged by size:
4351 2883 1748 556 280
ZraI GAC'GTC
Cuts at: 1931 1931
Size: 9818
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AscI AvrII BaeI BglI BmgBI BsiWI BsrGI
BssHII BstZ17I ClaI CspCI FseI FspAI MfeI NdeI
NotI NruI PacI PmlI PpuMI PsiI PspXI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI