Restriction map for
pF1KA0090
(Circular) (Six-base) MAPSORT of: pF1KA0090.seq Check: 2253 from: 1 to: 6089
pF1KA0090 6089 bp
With 182 enzymes: SgfI *
September 25, 2009 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2693 2693
Size: 6089
AccI GT'mk_AC
Cuts at: 173 2945 3127 173
Size: 2772 182 3135
Fragments arranged by size:
3135 2772 182
Acc65I G'GTAC_C
Cuts at: 3105 3105
Size: 6089
AclI AA'CG_TT
Cuts at: 5929 5929
Size: 6089
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 672 1169 1981 2553 2992 3914 4346 5093
Size: 107 497 812 572 439 922 432 747
Cuts at: 5093 5361 565
Size: 268 1293
Fragments arranged by size:
1293 922 812 747 572 497 439 432
268 107
AflIII A'CryG_T
Cuts at: 2990 3441 4545 5522 2990
Size: 451 1104 977 3557
Fragments arranged by size:
3557 1104 977 451
AgeI A'CCGG_T
Cuts at: 4477 4477
Size: 6089
AhdI GACnn_n'nnGTC
Cuts at: 1887 1887
Size: 6089
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 719 751 719
Size: 32 6057
AlwNI CAG_nnn'CTG
Cuts at: 774 3394 4961 774
Size: 2620 1567 1902
Fragments arranged by size:
2620 1902 1567
ApaI G_GGCC'C
Cuts at: 672 975 672
Size: 303 5786
ApaLI G'TGCA_C
Cuts at: 1863 4859 1863
Size: 2996 3093
ApoI r'AATT_y
Cuts at: 244 306 907 1519 1690 3093 5442 5698 244
Size: 62 601 612 171 1403 2349 256 635
Fragments arranged by size:
2349 1403 635 612 601 256 171 62
AseI AT'TA_AT
Cuts at: 20 20
Size: 6089
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6089
AvaI C'yCGr_G
Cuts at: 825 929 968 2752 2883 2939 3109 825
Size: 104 39 1784 131 56 170 3805
Fragments arranged by size:
3805 1784 170 131 104 56 39
BamHI G'GATC_C
Cuts at: 256 2739 3114 256
Size: 2483 375 3231
Fragments arranged by size:
3231 2483 375
BanI G'GyrC_C
Cuts at: 332 584 1762 3105 3519 3767 3802 332
Size: 252 1178 1343 414 248 35 2619
Fragments arranged by size:
2619 1343 1178 414 252 248 35
BanII G_rGCy'C
Cuts at: 672 832 975 2534 3103 4133 672
Size: 160 143 1559 569 1030 2628
Fragments arranged by size:
2628 1559 1030 569 160 143
BbeI G_GCGC'C
Cuts at: 336 3523 3771 336
Size: 3187 248 2654
Fragments arranged by size:
3187 2654 248
BbsI GAAGACnn'nnnn_
Cuts at: 185 556 1102 1905 185
Size: 371 546 803 4369
Fragments arranged by size:
4369 803 546 371
BciVI GTATCCnnnnn_n'
Cuts at: 3987 4748 6089 3987
Size: 761 1341 3987
Fragments arranged by size:
3987 1341 761
BclI T'GATC_A
Cuts at: 398 3035 3609 398
Size: 2637 574 2878
Fragments arranged by size:
2878 2637 574
BglI GCCn_nnn'nGGC
Cuts at: 112 1314 2647 112
Size: 1202 1333 3554
Fragments arranged by size:
3554 1333 1202
BglII A'GATC_T
Cuts at: 323 3604 5641 323
Size: 3281 2037 771
Fragments arranged by size:
3281 2037 771
BlpI GC'TnA_GC
Cuts at: 3201 3201
Size: 6089
Bme1580I G_kGCm'C
Cuts at: 352 587 672 975 1500 1867 2545 3714 3807
Size: 235 85 303 525 367 678 1169 93
Cuts at: 3807 4863 352
Size: 1056 1578
Fragments arranged by size:
1578 1169 1056 678 525 367 303 235
93 85
BmrI ACTGGGnnnn_n'
Cuts at: 326 471 949 969 1097 1502 1948 1987 2411
Size: 145 478 20 128 405 446 39 424
Cuts at: 2411 2481 3301 3382 3717 5861 326
Size: 70 820 81 335 2144 554
Fragments arranged by size:
2144 820 554 478 446 424 405 335
145 128 81 70 39 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1319 1773 4456 1319
Size: 454 2683 2952
Fragments arranged by size:
2952 2683 454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 800 877 1684 2548 2590 2726 2786 2911
Size: 239 77 807 864 42 136 60 125
Cuts at: 2911 561
Size: 3739
Fragments arranged by size:
3739 864 807 239 136 125 77 60
42
Bpu10I CC'TnA_GC
Cuts at: 1310 1310
Size: 6089
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 3265 4144 4636 4934 574
Size: 2691 879 492 298 1729
Fragments arranged by size:
2691 1729 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5793 5793
Size: 6089
BsaAI yAC'GTr
Cuts at: 2991 3442 2991
Size: 451 5638
BsaBI GATnn'nnATC
Cuts at: 6 3603 5640 6
Size: 3597 2037 455
Fragments arranged by size:
3597 2037 455
BsaHI Gr'CG_yC
Cuts at: 333 2396 3520 3768 4470 5966 333
Size: 2063 1124 248 702 1496 456
Fragments arranged by size:
2063 1496 1124 702 456 248
BsaWI w'CCGG_w
Cuts at: 3278 3799 4477 4751 4898 5309 3278
Size: 521 678 274 147 411 4058
Fragments arranged by size:
4058 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 313 343 548 578 719 749 824 854 313
Size: 30 205 30 141 30 75 30 5548
Fragments arranged by size:
5548 205 141 75 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1456 1797 2409 2794 1456
Size: 341 612 385 4751
Fragments arranged by size:
4751 612 385 341
BseYI C'CCAG_C
Cuts at: 944 1545 2588 3514 4272 4849 5345 944
Size: 601 1043 926 758 577 496 1688
Fragments arranged by size:
1688 1043 926 758 601 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 970 1393 2446 367
Size: 603 423 1053 4010
Fragments arranged by size:
4010 1053 603 423
BsiEI CG_ry'CG
Cuts at: 104 167 3631 3677 4885 104
Size: 63 3464 46 1208 1308
Fragments arranged by size:
3464 1308 1208 63 46
BsiHKAI G_wGCw'C
Cuts at: 832 1867 2258 2300 2347 2534 3103 3881 4071
Size: 1035 391 42 47 187 569 778 190
Cuts at: 4071 4863 832
Size: 792 2058
Fragments arranged by size:
2058 1035 792 778 569 391 190 187
47 42
BsmI GAATG_Cn'
Cuts at: 2460 2864 2460
Size: 404 5685
BsmBI CGTCTCn'nnnn_
Cuts at: 853 1577 853
Size: 724 5365
Bsp1286I G_dGCh'C
Cuts at: 352 587 672 832 975 1500 1867 2258 2300
Size: 235 85 160 143 525 367 391 42
Cuts at: 2300 2347 2534 2545 3103 3714 3807 3881 4071
Size: 47 187 11 558 611 93 74 190
Cuts at: 4071 4133 4863 352
Size: 62 730 1578
Fragments arranged by size:
1578 730 611 558 525 391 367 235
190 187 160 143 93 85 74 62
47 42 11
BspEI T'CCGG_A
Cuts at: 5309 5309
Size: 6089
BspHI T'CATG_A
Cuts at: 5265 5265
Size: 6089
BspMI ACCTGCnnnn'nnnn_
Cuts at: 732 2330 2693 3139 3353 3655 4036 732
Size: 1598 363 446 214 302 381 2785
Fragments arranged by size:
2785 1598 446 381 363 302 214
BsrBI CCG'CTC
Cuts at: 50 1298 1314 4381 4435 5919 6084 50
Size: 1248 16 3067 54 1484 165 55
Fragments arranged by size:
3067 1484 1248 165 55 54 16
BsrDI GCAATG_nn'
Cuts at: 4001 4001
Size: 6089
BsrFI r'CCGG_y
Cuts at: 4087 4268 4477 4087
Size: 181 209 5699
Fragments arranged by size:
5699 209 181
BsrGI T'GTAC_A
Cuts at: 2808 2808
Size: 6089
BssSI C'ACGA_G
Cuts at: 1240 4360 4718 1240
Size: 3120 358 2611
Fragments arranged by size:
3120 2611 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1983 3466 1983
Size: 1483 4606
BstBI TT'CG_AA
Cuts at: 2163 4450 2163
Size: 2287 3802
BstXI CCAn_nnnn'nTGG
Cuts at: 267 499 957 2325 2904 267
Size: 232 458 1368 579 3452
Fragments arranged by size:
3452 1368 579 458 232
BstYI r'GATC_y
Cuts at: 1 256 323 881 2169 2739 2756 3114 3581
Size: 255 67 558 1288 570 17 358 467
Cuts at: 3581 3604 3939 4185 5197 5283 5295 5641 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1288 1012 570 558 467 449 358 346
335 255 246 86 67 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 1728 2726 1728
Size: 998 5091
BtgI C'CryG_G
Cuts at: 107 167 760 2330 2642 107
Size: 60 593 1570 312 3554
Fragments arranged by size:
3554 1570 593 312 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 519 4039 4220 4383 519
Size: 3520 181 163 2225
Fragments arranged by size:
3520 2225 181 163
BtsI GCAGTG_nn'
Cuts at: 886 2256 2298 3332 886
Size: 1370 42 1034 3643
Fragments arranged by size:
3643 1370 1034 42
DraI TTT'AAA
Cuts at: 1815 3088 1815
Size: 1273 4816
DrdI GACnn_nn'nnGTC
Cuts at: 3795 4653 3795
Size: 858 5231
EaeI y'GGCC_r
Cuts at: 164 1679 2327 3674 3848 4239 4266 5611 164
Size: 1515 648 1347 174 391 27 1345 642
Fragments arranged by size:
1515 1347 1345 648 642 391 174 27
EagI C'GGCC_G
Cuts at: 164 3674 164
Size: 3510 2579
EarI CTCTTCn'nnn_
Cuts at: 4112 4322 4112
Size: 210 5879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4297 4607 4753 5899 4297
Size: 310 146 1146 4487
Fragments arranged by size:
4487 1146 310 146
EcoICRI GAG'CTC
Cuts at: 830 2532 3101 830
Size: 1702 569 3818
Fragments arranged by size:
3818 1702 569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 565 672 800 877 1169 1684 1981 2548
Size: 4 107 128 77 292 515 297 567
Cuts at: 2548 2553 2590 2726 2786 2911 2992 3914 4346
Size: 5 37 136 60 125 81 922 432
Cuts at: 4346 5093 5361 561
Size: 747 268 1289
Fragments arranged by size:
1289 922 747 567 515 432 297 292
268 136 128 125 107 81 77 60
37 5 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2725 2725
Size: 6089
EcoO109I rG'GnC_Cy
Cuts at: 453 669 933 2443 2601 3228 453
Size: 216 264 1510 158 627 3314
Fragments arranged by size:
3314 1510 627 264 216 158
EcoRI G'AATT_C
Cuts at: 1690 3093 1690
Size: 1403 4686
EcoRV GAT'ATC
Cuts at: 2155 2155
Size: 6089
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4108 4108
Size: 6089
FspI TGC'GCA
Cuts at: 3870 3870
Size: 6089
HaeII r_GCGC'y
Cuts at: 336 1968 3523 3771 4793 5772 336
Size: 1632 1555 248 1022 979 653
Fragments arranged by size:
1632 1555 1022 979 653 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 407 439 814 846 1368 1400 1873 1905 2838
Size: 32 375 32 522 32 473 32 933
Cuts at: 2838 2870 3931 3961 3963 3993 407
Size: 32 1061 30 2 30 2503
Fragments arranged by size:
2503 1061 933 522 473 375 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 342 3128 342
Size: 2786 3303
HindIII A'AGCT_T
Cuts at: 1735 1735
Size: 6089
KasI G'GCGC_C
Cuts at: 332 3519 3767 332
Size: 3187 248 2654
Fragments arranged by size:
3187 2654 248
KpnI G_GTAC'C
Cuts at: 3109 3109
Size: 6089
MluI A'CGCG_T
Cuts at: 5522 5522
Size: 6089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 948 1102 4760 4944 948
Size: 154 3658 184 2093
Fragments arranged by size:
3658 2093 184 154
MscI TGG'CCA
Cuts at: 1681 3850 1681
Size: 2169 3920
MslI CAynn'nnrTG
Cuts at: 4078 4205 4078
Size: 127 5962
MspA1I CmG'CkG
Cuts at: 169 2220 2592 3200 3514 3874 4887 5132 169
Size: 2051 372 608 314 360 1013 245 1126
Fragments arranged by size:
2051 1126 1013 608 372 360 314 245
NaeI GCC'GGC
Cuts at: 4270 4270
Size: 6089
NarI GG'CG_CC
Cuts at: 333 3520 3768 333
Size: 3187 248 2654
Fragments arranged by size:
3187 2654 248
NcoI C'CATG_G
Cuts at: 107 2642 107
Size: 2535 3554
NgoMIV G'CCGG_C
Cuts at: 4268 4268
Size: 6089
NotI GC'GGCC_GC
Cuts at: 164 164
Size: 6089
NspI r_CATG'y
Cuts at: 3142 3642 4549 3142
Size: 500 907 4682
Fragments arranged by size:
4682 907 500
PasI CC'CwG_GG
Cuts at: 2268 2268
Size: 6089
PciI A'CATG_T
Cuts at: 4545 4545
Size: 6089
PflMI CCAn_nnn'nTGG
Cuts at: 849 1772 849
Size: 923 5166
PmeI GTTT'AAAC
Cuts at: 3088 3088
Size: 6089
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 719 751 819 851 1824 1856 5228 5260 719
Size: 32 68 32 973 32 3372 32 1548
Fragments arranged by size:
3372 1548 973 68 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 933 2601 933
Size: 1668 4421
PspOMI G'GGCC_C
Cuts at: 668 971 668
Size: 303 5786
PspXI vC'TCGA_Gb
Cuts at: 2939 2939
Size: 6089
PstI C_TGCA'G
Cuts at: 518 770 893 1019 1110 1406 3136 3821 518
Size: 252 123 126 91 296 1730 685 2786
Fragments arranged by size:
2786 1730 685 296 252 126 123 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6089
PvuII CAG'CTG
Cuts at: 2592 3514 3874 2592
Size: 922 360 4807
Fragments arranged by size:
4807 922 360
RsrII CG'GwC_CG
Cuts at: 4284 4284
Size: 6089
SacI G_AGCT'C
Cuts at: 832 2534 3103 832
Size: 1702 569 3818
Fragments arranged by size:
3818 1702 569
SacII CC_GC'GG
Cuts at: 170 170
Size: 6089
SalI G'TCGA_C
Cuts at: 3126 3126
Size: 6089
SapI GCTCTTCn'nnn_
Cuts at: 4112 4322 4112
Size: 210 5879
SbfI CC_TGCA'GG
Cuts at: 518 3136 518
Size: 2618 3471
ScaI AGT'ACT
Cuts at: 2488 5606 2488
Size: 3118 2971
SfcI C'TryA_G
Cuts at: 33 514 766 889 1015 1106 1402 3132 3817
Size: 481 252 123 126 91 296 1730 685
Cuts at: 3817 4810 5001 33
Size: 993 191 1121
Fragments arranged by size:
1730 1121 993 685 481 296 252 191
126 123 91
SfoI GGC'GCC
Cuts at: 334 3521 3769 334
Size: 3187 248 2654
Fragments arranged by size:
3187 2654 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6089
SmaI CCC'GGG
Cuts at: 931 970 3111 931
Size: 39 2141 3909
Fragments arranged by size:
3909 2141 39
SmlI C'TyrA_G
Cuts at: 589 2939 3244 4159 4651 4913 589
Size: 2350 305 915 492 262 1765
Fragments arranged by size:
2350 1765 915 492 305 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6089
SphI G_CATG'C
Cuts at: 3142 3642 3142
Size: 500 5589
StyI C'CwwG_G
Cuts at: 107 239 2015 2498 2642 3223 3577 107
Size: 132 1776 483 144 581 354 2619
Fragments arranged by size:
2619 1776 581 483 354 144 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 585 2463 3672 3805 4264 4473 585
Size: 1878 1209 133 459 209 2201
Fragments arranged by size:
2201 1878 1209 459 209 133
TatI w'GTAC_w
Cuts at: 2214 2486 2808 5604 2214
Size: 272 322 2796 2699
Fragments arranged by size:
2796 2699 322 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 313 345 380 412 548 580 824 856 2043
Size: 32 35 32 136 32 244 32 1187
Cuts at: 2043 2075 2248 2280 5606 5638 313
Size: 32 173 32 3326 32 764
Fragments arranged by size:
3326 1187 764 244 173 136 35 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3886 3886
Size: 6089
XbaI T'CTAG_A
Cuts at: 3120 3120
Size: 6089
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2640 3584 2640
Size: 944 5145
XhoI C'TCGA_G
Cuts at: 2939 2939
Size: 6089
XmaI C'CCGG_G
Cuts at: 929 968 3109 929
Size: 39 2141 3909
Fragments arranged by size:
3909 2141 39
XmnI GAAnn'nnTTC
Cuts at: 1899 1899
Size: 6089
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BssHII BstEII
BstZ17I ClaI CspCI DraIII FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PfoI PmlI PshAI
PsiI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI