Restriction map for
pF1KA0072
(Circular) (Six-base) MAPSORT of: pF1KA0072.seq Check: 8440 from: 1 to: 4625
pF1KA0072 4625 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1055 1663 1055
Size: 608 4017
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 2882 3629 3897 2450
Size: 432 747 268 3178
Fragments arranged by size:
3178 747 432 268
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 336 1436 3013 336
Size: 1100 1577 1948
Fragments arranged by size:
1948 1577 1100
AlwNI CAG_nnn'CTG
Cuts at: 744 1930 3497 744
Size: 1186 1567 1872
Fragments arranged by size:
1872 1567 1186
ApaLI G'TGCA_C
Cuts at: 3395 3395
Size: 4625
ApoI r'AATT_y
Cuts at: 444 863 1629 3978 4234 444
Size: 419 766 2349 256 835
Fragments arranged by size:
2349 835 766 419 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 1283 1645 1283
Size: 362 4263
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 699 732 699
Size: 33 4592
BamHI G'GATC_C
Cuts at: 1075 1287 1650 1075
Size: 212 363 4050
Fragments arranged by size:
4050 363 212
BanI G'GyrC_C
Cuts at: 1641 2055 2303 2338 1641
Size: 414 248 35 3928
Fragments arranged by size:
3928 414 248 35
BanII G_rGCy'C
Cuts at: 1301 1389 1639 2669 1301
Size: 88 250 1030 3257
Fragments arranged by size:
3257 1030 250 88
BbeI G_GCGC'C
Cuts at: 2059 2307 2059
Size: 248 4377
BciVI GTATCCnnnnn_n'
Cuts at: 312 2523 3284 4625 312
Size: 2211 761 1341 312
Fragments arranged by size:
2211 1341 761 312
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglII A'GATC_T
Cuts at: 971 2140 4177 971
Size: 1169 2037 1419
Fragments arranged by size:
2037 1419 1169
BlpI GC'TnA_GC
Cuts at: 1737 1737
Size: 4625
Bme1580I G_kGCm'C
Cuts at: 2250 2343 3399 2250
Size: 93 1056 3476
Fragments arranged by size:
3476 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1162 1837 1918 2253 4397 1162
Size: 675 81 335 2144 1390
Fragments arranged by size:
2144 1390 675 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 331 604 1315 184
Size: 147 273 711 3494
Fragments arranged by size:
3494 711 273 147
Bpu10I CC'TnA_GC
Cuts at: 1226 1226
Size: 4625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 1801 2680 3172 3470 827
Size: 974 879 492 298 1982
Fragments arranged by size:
1982 974 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4329 4329
Size: 4625
BsaAI yAC'GTr
Cuts at: 345 1978 345
Size: 1633 2992
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 2056 2304 3006 4502 2056
Size: 248 702 1496 2179
Fragments arranged by size:
2179 1496 702 248
BsaWI w'CCGG_w
Cuts at: 336 1436 1814 2335 3013 3287 3434 3845 336
Size: 1100 378 521 678 274 147 411 1116
Fragments arranged by size:
1116 1100 678 521 411 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1604 1634 1604
Size: 30 4595
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 4625
BseYI C'CCAG_C
Cuts at: 737 2050 2808 3385 3881 737
Size: 1313 758 577 496 1481
Fragments arranged by size:
1481 1313 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2167 2213 3421 104
Size: 2063 46 1208 1308
Fragments arranged by size:
2063 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1301 1639 2417 2607 3399 1301
Size: 338 778 190 792 2527
Fragments arranged by size:
2527 792 778 338 190
BsmI GAATG_Cn'
Cuts at: 1167 1167
Size: 4625
Bsp1286I G_dGCh'C
Cuts at: 1301 1389 1639 2250 2343 2417 2607 2669 3399
Size: 88 250 611 93 74 190 62 730
Cuts at: 3399 1301
Size: 2527
Fragments arranged by size:
2527 730 611 250 190 93 88 74
62
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 1675 1889 2191 2572 377
Size: 1298 214 302 381 2430
Fragments arranged by size:
2430 1298 381 302 214
BsrBI CCG'CTC
Cuts at: 50 207 252 2917 2971 4455 4620 50
Size: 157 45 2665 54 1484 165 55
Fragments arranged by size:
2665 1484 165 157 55 54 45
BsrDI GCAATG_nn'
Cuts at: 1367 2537 1367
Size: 1170 3455
BsrFI r'CCGG_y
Cuts at: 336 1436 2623 2804 3013 336
Size: 1100 1187 181 209 1948
Fragments arranged by size:
1948 1187 1100 209 181
BssHII G'CGCG_C
Cuts at: 176 176
Size: 4625
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 201 2002 201
Size: 1801 2824
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstEII G'GTnAC_C
Cuts at: 732 732
Size: 4625
BstYI r'GATC_y
Cuts at: 1 971 1075 1287 1650 2117 2140 2475 2721
Size: 970 104 212 363 467 23 335 246
Cuts at: 2721 3733 3819 3831 4177 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 970 467 449 363 346 335 246
212 104 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 358 358
Size: 4625
BtgI C'CryG_G
Cuts at: 107 1305 107
Size: 1198 3427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 206 1868 206
Size: 1662 2963
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1023 1058 1023
Size: 35 4590
DraI TTT'AAA
Cuts at: 707 1624 707
Size: 917 3708
DraIII CAC_nnn'GTG
Cuts at: 189 189
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 1364 2210 2384 2775 2802 4147 1364
Size: 846 174 391 27 1345 1842
Fragments arranged by size:
1842 1345 846 391 174 27
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 259 1214 1306 2648 2858 259
Size: 955 92 1342 210 2026
Fragments arranged by size:
2026 1342 955 210 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 247 2833 3143 3289 4435 247
Size: 2586 310 146 1146 437
Fragments arranged by size:
2586 1146 437 310 146
EcoICRI GAG'CTC
Cuts at: 1299 1637 1299
Size: 338 4287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 331 604 1315 2450 2882 3629 3897 184
Size: 147 273 711 1135 432 747 268 912
Fragments arranged by size:
1135 912 747 711 432 273 268 147
EcoO109I rG'GnC_Cy
Cuts at: 1029 1764 1029
Size: 735 3890
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4625
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 130 160 2059 2307 3329 4308 130
Size: 30 1899 248 1022 979 447
Fragments arranged by size:
1899 1022 979 447 248 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 508 540 886 918 1008 1040 2467 2497 2499
Size: 32 346 32 90 32 1427 30 2
Cuts at: 2499 2529 508
Size: 30 2604
Fragments arranged by size:
2604 1427 346 90 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 366 1664 366
Size: 1298 3327
HindIII A'AGCT_T
Cuts at: 1457 1457
Size: 4625
KasI G'GCGC_C
Cuts at: 2055 2303 2055
Size: 248 4377
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MfeI C'AATT_G
Cuts at: 1511 1511
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1044 3296 3480 1044
Size: 2252 184 2189
Fragments arranged by size:
2252 2189 184
MscI TGG'CCA
Cuts at: 2386 2386
Size: 4625
MslI CAynn'nnrTG
Cuts at: 392 2614 2741 392
Size: 2222 127 2276
Fragments arranged by size:
2276 2222 127
MspA1I CmG'CkG
Cuts at: 162 1736 2050 2410 3423 3668 162
Size: 1574 314 360 1013 245 1119
Fragments arranged by size:
1574 1119 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2806 2806
Size: 4625
NarI GG'CG_CC
Cuts at: 2056 2304 2056
Size: 248 4377
NcoI C'CATG_G
Cuts at: 107 107
Size: 4625
NgoMIV G'CCGG_C
Cuts at: 2804 2804
Size: 4625
NspI r_CATG'y
Cuts at: 1678 2178 3085 1678
Size: 500 907 3218
Fragments arranged by size:
3218 907 500
PasI CC'CwG_GG
Cuts at: 1382 1382
Size: 4625
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 123 330 123
Size: 207 4418
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PmlI CAC'GTG
Cuts at: 345 345
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 1029 1029
Size: 4625
PstI C_TGCA'G
Cuts at: 197 374 1672 2357 197
Size: 177 1298 685 2465
Fragments arranged by size:
2465 1298 685 177
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 2050 2410 2050
Size: 360 4265
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1301 1639 1301
Size: 338 4287
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 259 1306 2648 2858 259
Size: 1047 1342 210 2026
Fragments arranged by size:
2026 1342 1047 210
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SfcI C'TryA_G
Cuts at: 33 193 370 527 1052 1668 2353 3346 3537
Size: 160 177 157 525 616 685 993 191
Cuts at: 3537 33
Size: 1121
Fragments arranged by size:
1121 993 685 616 525 191 177 160
157
SfoI GGC'GCC
Cuts at: 2057 2305 2057
Size: 248 4377
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 842 1780 2695 3187 3449 842
Size: 938 915 492 262 2018
Fragments arranged by size:
2018 938 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1482 67
Size: 1415 3210
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StyI C'CwwG_G
Cuts at: 107 1759 2113 107
Size: 1652 354 2619
Fragments arranged by size:
2619 1652 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2208 2341 2800 3009 2208
Size: 133 459 209 3824
Fragments arranged by size:
3824 459 209 133
TatI w'GTAC_w
Cuts at: 1214 4140 1214
Size: 2926 1699
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4593
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 726 2120 726
Size: 1394 3231
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
XmnI GAAnn'nnTTC
Cuts at: 338 338
Size: 4625
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsgI BsiWI BsmBI BsrGI BstXI BstZ17I
ClaI EcoNI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PfoI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI