Restriction map for
pF1KA0066
(Circular) (Six-base) MAPSORT of: pF1KA0066.seq Check: 8692 from: 1 to: 6056
pF1KA0066 6056 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3010 3010
Size: 6056
AccI GT'mk_AC
Cuts at: 3094 3094
Size: 6056
Acc65I G'GTAC_C
Cuts at: 3072 3072
Size: 6056
AclI AA'CG_TT
Cuts at: 5896 5896
Size: 6056
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 1904 2093 2498 2762 3881 4313 5060 5328
Size: 198 189 405 264 1119 432 747 268
Cuts at: 5328 1706
Size: 2434
Fragments arranged by size:
2434 1119 747 432 405 268 264 198
189
AflIII A'CryG_T
Cuts at: 3408 4512 5489 3408
Size: 1104 977 3975
Fragments arranged by size:
3975 1104 977
AgeI A'CCGG_T
Cuts at: 2755 4444 2755
Size: 1689 4367
AleI CACnn'nnGTG
Cuts at: 1292 2938 1292
Size: 1646 4410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2281 2956 2281
Size: 675 5381
AlwNI CAG_nnn'CTG
Cuts at: 3361 4928 3361
Size: 1567 4489
ApaLI G'TGCA_C
Cuts at: 4826 4826
Size: 6056
ApoI r'AATT_y
Cuts at: 299 611 1096 1106 1555 1858 1944 2920 3060
Size: 312 485 10 449 303 86 976 140
Cuts at: 3060 5409 5665 299
Size: 2349 256 690
Fragments arranged by size:
2349 976 690 485 449 312 303 256
140 86 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 6056
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6056
AvaI C'yCGr_G
Cuts at: 2219 3076 2219
Size: 857 5199
BamHI G'GATC_C
Cuts at: 1014 3081 1014
Size: 2067 3989
BanI G'GyrC_C
Cuts at: 594 916 1776 2441 3022 3072 3486 3734 3769
Size: 322 860 665 581 50 414 248 35
Cuts at: 3769 594
Size: 2881
Fragments arranged by size:
2881 860 665 581 414 322 248 50
35
BanII G_rGCy'C
Cuts at: 128 2112 2646 2863 3070 4100 128
Size: 1984 534 217 207 1030 2084
Fragments arranged by size:
2084 1984 1030 534 217 207
BbeI G_GCGC'C
Cuts at: 3490 3738 3490
Size: 248 5808
BbsI GAAGACnn'nnnn_
Cuts at: 1433 2588 1433
Size: 1155 4901
BbvCI CC'TCA_GC
Cuts at: 842 2975 842
Size: 2133 3923
BciVI GTATCCnnnnn_n'
Cuts at: 3954 4715 6056 3954
Size: 761 1341 3954
Fragments arranged by size:
3954 1341 761
BclI T'GATC_A
Cuts at: 1505 3576 1505
Size: 2071 3985
BglI GCCn_nnn'nGGC
Cuts at: 2815 2815
Size: 6056
BglII A'GATC_T
Cuts at: 1626 2052 2703 3571 5608 1626
Size: 426 651 868 2037 2074
Fragments arranged by size:
2074 2037 868 651 426
BlpI GC'TnA_GC
Cuts at: 2336 3168 2336
Size: 832 5224
Bme1580I G_kGCm'C
Cuts at: 2289 3681 3774 4830 2289
Size: 1392 93 1056 3515
Fragments arranged by size:
3515 1392 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3268 3349 3684 5828 3268
Size: 81 335 2144 3496
Fragments arranged by size:
3496 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2026 4423 2026
Size: 2397 3659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 836 1860 2752 764
Size: 72 1024 892 4068
Fragments arranged by size:
4068 1024 892 72
Bpu10I CC'TnA_GC
Cuts at: 842 2975 842
Size: 2133 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2461 3232 4111 4603 4901 2461
Size: 771 879 492 298 3616
Fragments arranged by size:
3616 879 771 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1760 5760 1760
Size: 4000 2056
BsaAI yAC'GTr
Cuts at: 481 1696 3409 481
Size: 1215 1713 3128
Fragments arranged by size:
3128 1713 1215
BsaBI GATnn'nnATC
Cuts at: 6 1013 3570 5607 6
Size: 1007 2557 2037 455
Fragments arranged by size:
2557 2037 1007 455
BsaHI Gr'CG_yC
Cuts at: 3487 3735 4437 5933 3487
Size: 248 702 1496 3610
Fragments arranged by size:
3610 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2755 3245 3766 4444 4718 4865 5276 2755
Size: 490 521 678 274 147 411 3535
Fragments arranged by size:
3535 678 521 490 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 866 896 866
Size: 30 6026
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 890 1315 2251 2704 2854 890
Size: 425 936 453 150 4092
Fragments arranged by size:
4092 936 453 425 150
BseYI C'CCAG_C
Cuts at: 420 3481 4239 4816 5312 420
Size: 3061 758 577 496 1164
Fragments arranged by size:
3061 1164 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1442 2386 2780 491
Size: 951 944 394 3767
Fragments arranged by size:
3767 951 944 394
BsiEI CG_ry'CG
Cuts at: 104 3598 3644 4852 104
Size: 3494 46 1208 1308
Fragments arranged by size:
3494 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 453 2124 2646 3070 3848 4038 4830 453
Size: 1671 522 424 778 190 792 1679
Fragments arranged by size:
1679 1671 792 778 522 424 190
BsmI GAATG_Cn'
Cuts at: 1873 2811 1873
Size: 938 5118
Bsp1286I G_dGCh'C
Cuts at: 128 453 2112 2124 2289 2646 2863 3070 3681
Size: 325 1659 12 165 357 217 207 611
Cuts at: 3681 3774 3848 4038 4100 4830 128
Size: 93 74 190 62 730 1354
Fragments arranged by size:
1659 1354 730 611 357 325 217 207
190 165 93 74 62 12
BspEI T'CCGG_A
Cuts at: 5276 5276
Size: 6056
BspHI T'CATG_A
Cuts at: 5232 5232
Size: 6056
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2433 3010 3106 3320 3622 4003 2433
Size: 577 96 214 302 381 4486
Fragments arranged by size:
4486 577 381 302 214 96
BsrBI CCG'CTC
Cuts at: 50 4348 4402 5886 6051 50
Size: 4298 54 1484 165 55
Fragments arranged by size:
4298 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 453 772 2265 2633 3968 453
Size: 319 1493 368 1335 2541
Fragments arranged by size:
2541 1493 1335 368 319
BsrFI r'CCGG_y
Cuts at: 1215 2755 4054 4235 4444 1215
Size: 1540 1299 181 209 2827
Fragments arranged by size:
2827 1540 1299 209 181
BsrGI T'GTAC_A
Cuts at: 345 2984 345
Size: 2639 3417
BssSI C'ACGA_G
Cuts at: 4327 4685 4327
Size: 358 5698
BstAPI GCAn_nnn'nTGC
Cuts at: 417 2434 3433 417
Size: 2017 999 3040
Fragments arranged by size:
3040 2017 999
BstBI TT'CG_AA
Cuts at: 677 1050 4417 677
Size: 373 3367 2316
Fragments arranged by size:
3367 2316 373
BstYI r'GATC_y
Cuts at: 1 927 1014 1626 2052 2703 3081 3548 3571
Size: 926 87 612 426 651 378 467 23
Cuts at: 3571 3906 4152 5164 5250 5262 5608 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 926 651 612 467 449 426 378
346 335 246 87 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1931 1931
Size: 6056
BtgI C'CryG_G
Cuts at: 107 1518 107
Size: 1411 4645
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4006 4187 4350 4006
Size: 181 163 5712
Fragments arranged by size:
5712 181 163
BtsI GCAGTG_nn'
Cuts at: 161 1262 1987 3299 161
Size: 1101 725 1312 2918
Fragments arranged by size:
2918 1312 1101 725
DraI TTT'AAA
Cuts at: 1104 3055 1104
Size: 1951 4105
DraIII CAC_nnn'GTG
Cuts at: 514 514
Size: 6056
DrdI GACnn_nn'nnGTC
Cuts at: 3762 4620 3762
Size: 858 5198
EaeI y'GGCC_r
Cuts at: 2913 3641 3815 4206 4233 5578 2913
Size: 728 174 391 27 1345 3391
Fragments arranged by size:
3391 1345 728 391 174 27
EagI C'GGCC_G
Cuts at: 3641 3641
Size: 6056
EarI CTCTTCn'nnn_
Cuts at: 278 2057 2228 2760 2845 4079 4289 278
Size: 1779 171 532 85 1234 210 2045
Fragments arranged by size:
2045 1779 1234 532 210 171 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4264 4574 4720 5866 4264
Size: 310 146 1146 4454
Fragments arranged by size:
4454 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2644 3068 2644
Size: 424 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 836 1706 1860 1904 2093 2498 2752 2762
Size: 72 870 154 44 189 405 254 10
Cuts at: 2762 3881 4313 5060 5328 764
Size: 1119 432 747 268 1492
Fragments arranged by size:
1492 1119 870 747 432 405 268 254
189 154 72 44 10
EcoNI CCTnn'n_nnAGG
Cuts at: 1549 1549
Size: 6056
EcoO109I rG'GnC_Cy
Cuts at: 655 1927 3195 655
Size: 1272 1268 3516
Fragments arranged by size:
3516 1272 1268
EcoRI G'AATT_C
Cuts at: 2920 3060 2920
Size: 140 5916
EcoRV GAT'ATC
Cuts at: 738 738
Size: 6056
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4075 4075
Size: 6056
FspI TGC'GCA
Cuts at: 2932 3837 2932
Size: 905 5151
HaeII r_GCGC'y
Cuts at: 3490 3738 4760 5739 3490
Size: 248 1022 979 3807
Fragments arranged by size:
3807 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 744 776 1204 1236 2037 2069 2124
Size: 32 597 32 428 32 801 32 55
Cuts at: 2124 2143 2156 2175 3898 3928 3930 3960 115
Size: 19 13 19 1723 30 2 30 2211
Fragments arranged by size:
2211 1723 801 597 428 55 32 32
32 32 30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 1127 3095 1127
Size: 1968 4088
KasI G'GCGC_C
Cuts at: 3486 3734 3486
Size: 248 5808
KpnI G_GTAC'C
Cuts at: 3076 3076
Size: 6056
MluI A'CGCG_T
Cuts at: 5489 5489
Size: 6056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 864 4727 4911 864
Size: 3863 184 2009
Fragments arranged by size:
3863 2009 184
MscI TGG'CCA
Cuts at: 3817 3817
Size: 6056
MslI CAynn'nnrTG
Cuts at: 1292 2938 4045 4172 1292
Size: 1646 1107 127 3176
Fragments arranged by size:
3176 1646 1107 127
MspA1I CmG'CkG
Cuts at: 2470 2979 3167 3481 3841 4854 5099 2470
Size: 509 188 314 360 1013 245 3427
Fragments arranged by size:
3427 1013 509 360 314 245 188
NaeI GCC'GGC
Cuts at: 1217 4237 1217
Size: 3020 3036
NarI GG'CG_CC
Cuts at: 3487 3735 3487
Size: 248 5808
NcoI C'CATG_G
Cuts at: 107 1518 107
Size: 1411 4645
NgoMIV G'CCGG_C
Cuts at: 1215 4235 1215
Size: 3020 3036
NspI r_CATG'y
Cuts at: 2130 2776 3109 3609 4516 2130
Size: 646 333 500 907 3670
Fragments arranged by size:
3670 907 646 500 333
PciI A'CATG_T
Cuts at: 4512 4512
Size: 6056
PflMI CCAn_nnn'nTGG
Cuts at: 1381 1381
Size: 6056
PfoI T'CCnGG_A
Cuts at: 1602 1837 1602
Size: 235 5821
PmeI GTTT'AAAC
Cuts at: 3055 3055
Size: 6056
PmlI CAC'GTG
Cuts at: 1696 1696
Size: 6056
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5195 5227 5195
Size: 32 6024
PpuMI rG'GwC_Cy
Cuts at: 655 1927 655
Size: 1272 4784
PstI C_TGCA'G
Cuts at: 2430 2823 3103 3788 2430
Size: 393 280 685 4698
Fragments arranged by size:
4698 685 393 280
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6056
PvuII CAG'CTG
Cuts at: 2470 3481 3841 2470
Size: 1011 360 4685
Fragments arranged by size:
4685 1011 360
RsrII CG'GwC_CG
Cuts at: 4251 4251
Size: 6056
SacI G_AGCT'C
Cuts at: 2646 3070 2646
Size: 424 5632
SalI G'TCGA_C
Cuts at: 3093 3093
Size: 6056
SapI GCTCTTCn'nnn_
Cuts at: 2057 4079 4289 2057
Size: 2022 210 3824
Fragments arranged by size:
3824 2022 210
SbfI CC_TGCA'GG
Cuts at: 3103 3103
Size: 6056
ScaI AGT'ACT
Cuts at: 5573 5573
Size: 6056
SfcI C'TryA_G
Cuts at: 33 1657 2426 2819 3099 3784 4777 4968 33
Size: 1624 769 393 280 685 993 191 1121
Fragments arranged by size:
1624 1121 993 769 685 393 280 191
SfoI GGC'GCC
Cuts at: 3488 3736 3488
Size: 248 5808
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6056
SgrAI Cr'CCGG_yG
Cuts at: 2755 2755
Size: 6056
SmaI CCC'GGG
Cuts at: 2221 3078 2221
Size: 857 5199
SmlI C'TyrA_G
Cuts at: 2476 3211 4126 4618 4880 2476
Size: 735 915 492 262 3652
Fragments arranged by size:
3652 915 735 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6056
SphI G_CATG'C
Cuts at: 2130 3109 3609 2130
Size: 979 500 4577
Fragments arranged by size:
4577 979 500
StyI C'CwwG_G
Cuts at: 107 572 1518 3190 3544 107
Size: 465 946 1672 354 2619
Fragments arranged by size:
2619 1672 946 465 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 988 2636 3639 3772 4231 4440 988
Size: 1648 1003 133 459 209 2604
Fragments arranged by size:
2604 1648 1003 459 209 133
TatI w'GTAC_w
Cuts at: 345 710 810 2472 2984 5571 345
Size: 365 100 1662 512 2587 830
Fragments arranged by size:
2587 1662 830 512 365 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2973 3005 5573 5605 2973
Size: 32 2568 32 3424
Fragments arranged by size:
3424 2568 32 32
Tth111I GACn'n_nGTC
Cuts at: 3853 3853
Size: 6056
XbaI T'CTAG_A
Cuts at: 3087 3087
Size: 6056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 3551 2309
Size: 1242 4814
XmaI C'CCGG_G
Cuts at: 2219 3076 2219
Size: 857 5199
XmnI GAAnn'nnTTC
Cuts at: 683 1389 1559 2603 683
Size: 706 170 1044 4136
Fragments arranged by size:
4136 1044 706 170
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BssHII
BstEII BstXI BstZ17I ClaI CspCI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
ZraI